UA41863C2 - A method for obtaining heterologous polypeptide in eukaryotic microorganisms - Google Patents

A method for obtaining heterologous polypeptide in eukaryotic microorganisms

Info

Publication number
UA41863C2
UA41863C2 UA4203156A UA4203156A UA41863C2 UA 41863 C2 UA41863 C2 UA 41863C2 UA 4203156 A UA4203156 A UA 4203156A UA 4203156 A UA4203156 A UA 4203156A UA 41863 C2 UA41863 C2 UA 41863C2
Authority
UA
Ukraine
Prior art keywords
heterologous polypeptide
signal peptide
eukaryotic microorganisms
bari
lys
Prior art date
Application number
UA4203156A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Вівіан Л. Маккей
Вивиан Л. Маккей
Original Assignee
Займодженетікс Інк.
ЗАЙМОДЖЕНЕТИКС Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Займодженетікс Інк., ЗАЙМОДЖЕНЕТИКС Инк. filed Critical Займодженетікс Інк.
Publication of UA41863C2 publication Critical patent/UA41863C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A method for obtaining heterologous polypeptide in eukaryotic microorganisms providing construction of recombinant plasmid DNA containing promoter, sequence coding signal peptide and structural gene, transformation of obtained recombinant DNA of strains-recipients, cultivation of transformants, isolation and purification of purpose product. Constructed is recombinant plasmid DNA containing promoter of BARI Saccharomyces cerevisiae or promoter of alcohol dehydronase I S.cerevisiae, part of sequence of gene BARI, coding signal peptide, and site of detachment of signal peptide KEX2 from heterologous polypeptide, which represents Arg-Arg or Lys-Lys.
UA4203156A 1985-10-25 1986-10-20 A method for obtaining heterologous polypeptide in eukaryotic microorganisms UA41863C2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79130585A 1985-10-25 1985-10-25
PCT/US1986/002198 WO1987002670A1 (en) 1985-10-25 1986-10-20 Method of using bar1 for secreting foreign proteins

Publications (1)

Publication Number Publication Date
UA41863C2 true UA41863C2 (en) 2001-10-15

Family

ID=25153298

Family Applications (1)

Application Number Title Priority Date Filing Date
UA4203156A UA41863C2 (en) 1985-10-25 1986-10-20 A method for obtaining heterologous polypeptide in eukaryotic microorganisms

Country Status (13)

Country Link
EP (1) EP0243465A1 (en)
JP (1) JP2523562B2 (en)
CN (1) CN1027179C (en)
AU (2) AU6543286A (en)
CA (1) CA1316133C (en)
CZ (1) CZ284251B6 (en)
DK (1) DK320287A (en)
FI (1) FI872801A (en)
HU (1) HU206897B (en)
IE (1) IE63822B1 (en)
SK (1) SK279041B6 (en)
UA (1) UA41863C2 (en)
WO (1) WO1987002670A1 (en)

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DK82893D0 (en) * 1993-07-08 1993-07-08 Novo Nordisk As PEPTIDE
CN102080070B (en) 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 new endoglucanase
DK1975177T3 (en) 1996-03-01 2011-07-25 Novo Nordisk As Appetite suppressant peptide, formulations thereof and use thereof
CA2395713C (en) 2000-01-10 2012-04-10 Torben Lauesgaard Nissen G-csf conjugates
WO2001058935A2 (en) 2000-02-11 2001-08-16 Maxygen Aps FACTOR VII OR VIIa-LIKE MOLECULES
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BR0208203A (en) 2001-03-22 2005-04-19 Novo Nordisk Healthcare Ag Factor vii polypeptide, factor vii derivative, composition, pharmaceutical composition, polynucleotide construction, eukaryotic host cell, transgenic animal, transgenic plant, and methods for producing factor vii polypeptide and a factor vii derivative, use of a derivative of vii, methods for treating bleeding episodes or bleeding disorders in a patient or for enhancing the normal hemostatic system and inhibiting thrombus formation in a patient
US6534059B2 (en) 2001-06-05 2003-03-18 Advanced Biotherapy, Inc. Compositions and methods for treating hyperimmune response in the eye
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JP2008538290A (en) 2005-04-18 2008-10-23 ノボ ノルディスク アクティーゼルスカブ IL-21 mutant
WO2007020256A1 (en) 2005-08-16 2007-02-22 Novo Nordisk A/S Method for making mature insulin polypeptides
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WO2007103447A2 (en) 2006-03-06 2007-09-13 Humagene, Inc. A method for the preparation of recombinant human thrombin
AU2007292903B2 (en) 2006-09-08 2012-03-29 Ambrx, Inc. Modified human plasma polypeptide or Fc scaffolds and their uses
JP5503968B2 (en) 2006-09-27 2014-05-28 ノボ・ノルデイスク・エー／エス Method for producing mature insulin polypeptide
WO2008137471A2 (en) 2007-05-02 2008-11-13 Ambrx, Inc. Modified interferon beta polypeptides and their uses
US8946148B2 (en) 2007-11-20 2015-02-03 Ambrx, Inc. Modified insulin polypeptides and their uses
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US8349325B2 (en) 2008-12-23 2013-01-08 Abbott Laboratories Soluble FMS-like tyrosine kinase-1 (sFLT-1) antibody and related composition, kit, methods of using, and materials and method for making
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EP3815708A1 (en) 2010-03-05 2021-05-05 Omeros Corporation Chimeric inhibitor molecules of complement activation
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US8993248B2 (en) 2011-12-31 2015-03-31 Abbott Laboratories Truncated human vitamin D binding protein and mutation and fusion thereof and related materials and methods of use
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Also Published As

Publication number Publication date
DK320287D0 (en) 1987-06-23
JP2523562B2 (en) 1996-08-14
SK776486A3 (en) 1998-06-03
SK279041B6 (en) 1998-06-03
CA1316133C (en) 1993-04-13
FI872801A0 (en) 1987-06-24
EP0243465A1 (en) 1987-11-04
HUT43624A (en) 1987-11-30
AU676132B2 (en) 1997-03-06
CZ284251B6 (en) 1998-10-14
IE63822B1 (en) 1995-06-14
AU6543286A (en) 1987-05-19
CN86107554A (en) 1987-08-26
JPS63501614A (en) 1988-06-23
IE862804L (en) 1987-04-25
DK320287A (en) 1987-06-23
WO1987002670A1 (en) 1987-05-07
HU206897B (en) 1993-01-28
AU7400391A (en) 1991-07-18
FI872801A (en) 1987-06-24
CN1027179C (en) 1994-12-28
CZ776486A3 (en) 1996-09-11

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