ES2056785T3 - Metodo de utilizacion de bar1 para la secrecion de proteinas extrañas. - Google Patents
Metodo de utilizacion de bar1 para la secrecion de proteinas extrañas.Info
- Publication number
- ES2056785T3 ES2056785T3 ES86114769T ES86114769T ES2056785T3 ES 2056785 T3 ES2056785 T3 ES 2056785T3 ES 86114769 T ES86114769 T ES 86114769T ES 86114769 T ES86114769 T ES 86114769T ES 2056785 T3 ES2056785 T3 ES 2056785T3
- Authority
- ES
- Spain
- Prior art keywords
- foreign
- bar1
- secretion
- signal peptide
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
Abstract
SE DA UN METODO PARA LA PRODUCCION DE UNA PROTEINA EXTRAÑA EN UN ORGANISMO HUESPED, POR MEDIO DEL CUAL ES PROCESADA A TRAVES DEL CONDUCTO SECRETORIO DEL MISMO. LA PRODUCCION Y EL PROCESADO SE REALIZAN POR TRANSFORMACION DEL ORGANISMO HUESPED CON UN VECTOR PORTADOR QUE CONTIENE AL MENOS LA REGION CIFRADA (O EN CLAVE) DEL PEPTIDO SEÑALIZADO DEL FERMENTO S. CEREVISIAE BARI Y UN GEN FUSIONADO A OTRO GEN ESTRUCTURAL EXTRAÑO. LOS DNA CONSTRUCTORES Y TRANSFORMANTES TAMBIEN SON PREPARADAS CONTENIENDO UNA REGION DEL GEN BARI CODIFICANDO EL PEPTIDO SEÑALIZADOR, UN LUGAR KEX2, Y UN GEN ESTRUCTURAL EXTRAÑO. EN PARTICULAR, LA PROINSULINA ES PRODUCIDA Y SEGREGADA DE LA SCHIZOSACCHAROMICES POMPE Y SACCHAROMICES CEREVISIAE.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US79130585A | 1985-10-25 | 1985-10-25 | |
PCT/US1986/002198 WO1987002670A1 (en) | 1985-10-25 | 1986-10-20 | Method of using bar1 for secreting foreign proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2056785T3 true ES2056785T3 (es) | 1994-10-16 |
Family
ID=26774053
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES86114769T Expired - Lifetime ES2056785T3 (es) | 1985-10-25 | 1986-10-24 | Metodo de utilizacion de bar1 para la secrecion de proteinas extrañas. |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0220689B1 (es) |
AT (1) | ATE107357T1 (es) |
DE (1) | DE3689918T2 (es) |
ES (1) | ES2056785T3 (es) |
RU (1) | RU2091490C1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3887425T2 (de) * | 1987-10-02 | 1994-06-23 | Zymogenetics Inc | BAR1-Sekretions-Signal-Sequenz. |
EP0387319B1 (en) * | 1988-07-23 | 1996-03-06 | Delta Biotechnology Limited | Secretory leader sequences |
US6348327B1 (en) | 1991-12-06 | 2002-02-19 | Genentech, Inc. | Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells |
FI92601C (fi) * | 1992-03-11 | 1994-12-12 | Marja Makarow | Menetelmä hyötyproteiinien erittämiseksi hiivoista |
IT1255697B (it) * | 1992-09-29 | 1995-11-10 | Enichem Spa | Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano |
DE4441197C1 (de) * | 1994-11-18 | 1996-03-28 | Deutsches Krebsforsch | Expressionsplasmide für Hefe |
CA2198966C (en) * | 1996-03-04 | 2011-06-21 | Yuji Suzuki | Method for cleaving chimeric protein using processing enzyme |
AU7449400A (en) | 1999-09-30 | 2001-04-30 | Ajinomoto Co., Inc. | Process for producing transglutaminase |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK172738B1 (da) * | 1983-04-07 | 1999-06-21 | Chiron Corp | Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden |
US4613572A (en) * | 1983-08-15 | 1986-09-23 | Kansas State University Research Foundation | Yeast BAR1 gene plasmid |
DE3537176C2 (de) * | 1984-10-18 | 1994-06-09 | Zymogenetics Inc | Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung |
-
1986
- 1986-10-20 RU SU864203156A patent/RU2091490C1/ru not_active IP Right Cessation
- 1986-10-24 DE DE3689918T patent/DE3689918T2/de not_active Expired - Fee Related
- 1986-10-24 AT AT86114769T patent/ATE107357T1/de not_active IP Right Cessation
- 1986-10-24 ES ES86114769T patent/ES2056785T3/es not_active Expired - Lifetime
- 1986-10-24 EP EP86114769A patent/EP0220689B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0220689A2 (en) | 1987-05-06 |
RU2091490C1 (ru) | 1997-09-27 |
EP0220689B1 (en) | 1994-06-15 |
EP0220689A3 (en) | 1988-08-31 |
ATE107357T1 (de) | 1994-07-15 |
DE3689918D1 (de) | 1994-07-21 |
DE3689918T2 (de) | 1994-10-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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