TWI394839B - 快速結果雜交捕捉法及系統 - Google Patents
快速結果雜交捕捉法及系統 Download PDFInfo
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
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| US8357538B2 (en) * | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
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| US9107652B2 (en) * | 2009-11-19 | 2015-08-18 | Qiagen Gaithersburg, Inc. | Sampling devices and methods |
| AU2011210734B2 (en) * | 2010-01-29 | 2017-02-09 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
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| US9376727B2 (en) | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
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| AU2012242847B2 (en) | 2011-04-15 | 2017-01-19 | The Johns Hopkins University | Safe sequencing system |
| US11485968B2 (en) | 2012-02-13 | 2022-11-01 | Neumodx Molecular, Inc. | Microfluidic cartridge for processing and detecting nucleic acids |
| US9637775B2 (en) | 2012-02-13 | 2017-05-02 | Neumodx Molecular, Inc. | System and method for processing biological samples |
| US11931740B2 (en) | 2012-02-13 | 2024-03-19 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| US9604213B2 (en) | 2012-02-13 | 2017-03-28 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| CN104254595A (zh) | 2012-02-13 | 2014-12-31 | 纽莫德克斯莫勒库拉尔公司 | 用于处理和检测核酸的微流体盒 |
| WO2013123440A1 (en) * | 2012-02-17 | 2013-08-22 | Institute For Systems Biology | Rnase h-based rna profiling |
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| EP2912174B1 (en) | 2012-10-25 | 2019-06-19 | Neumodx Molecular, Inc. | Method and materials for isolation of nucleic acid materials |
| EP2912468B1 (en) | 2012-10-29 | 2018-09-12 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| JP6678583B2 (ja) | 2013-12-20 | 2020-04-22 | バイオメッド バレー ディスカバリーズ，インコーポレイティド | ２型ｍｅｋ阻害剤およびｅｒｋ阻害剤の組み合わせを用いるがん処置 |
| EP4062917B1 (en) | 2013-12-20 | 2025-12-03 | Biomed Valley Discoveries, Inc. | Cancer treatments using combinations of pi3k/akt pathway and erk inhibitors |
| BR112016014481B1 (pt) | 2013-12-20 | 2022-11-08 | Biomed Valley Discoveries, Inc | Uso de uma composição farmacêutica, método in vitro para efetuar a morte de células cancerosas, e kit |
| WO2015095842A2 (en) | 2013-12-20 | 2015-06-25 | Biomed Valley Discoveries, Inc. | Methods and compositions for treating non-erk mapk pathway inhibitor-resistant cancers |
| AU2014368927B2 (en) | 2013-12-20 | 2018-10-25 | Biomed Valley Discoveries, Inc. | Cancer treatments using combinations of CDK and ERK inhibitors |
| CN104020291B (zh) * | 2014-06-26 | 2016-03-30 | 南京大学 | 一种基于酶联免疫分析的hpv核酸检测试剂盒及其应用 |
| JP6682734B2 (ja) * | 2015-01-27 | 2020-04-15 | ビージーアイ シェンチェン | 生体試料を保存するための安定化剤 |
| EP3314269A4 (en) | 2015-06-26 | 2019-01-23 | Abbott Laboratories | REACTION TANK EXCHANGER DEVICE FOR DIAGNOSTIC ANALYZER |
| WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| WO2018160907A1 (en) * | 2017-03-03 | 2018-09-07 | Counsyl, Inc. | Extraction of nucleic acids for reduced probe count variability |
| IL319255A (en) | 2017-08-07 | 2025-04-01 | Univ Johns Hopkins | Methods and materials for cancer assessment and treatment |
| JP2022535747A (ja) * | 2019-05-28 | 2022-08-10 | ケース ウエスタン リザーブ ユニバーシティ | Ｄｎａのメチル化を保存するための組成物および方法 |
| IL295297A (en) | 2020-02-14 | 2022-10-01 | Univ Johns Hopkins | Methods and materials for assessing nucleic acids |
| AU2021281223A1 (en) | 2020-05-27 | 2023-01-05 | Case Western Reserve University | Compositions and methods for preserving DNA methylation |
| US11421217B1 (en) * | 2021-04-07 | 2022-08-23 | Convergent Genomics, Inc. | Methods and compositions for collection and preservation of biologicals |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993010263A1 (en) * | 1991-11-14 | 1993-05-27 | Digene Diagnostics, Inc. | Non-radioactive hybridization assay and kit |
Family Cites Families (195)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732847A (en) | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
| FI63596C (fi) | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| US4486536A (en) | 1982-05-28 | 1984-12-04 | Smithkline Diagnostics, Inc. | Specimen slide for occult blood testing |
| US4865980A (en) | 1982-12-29 | 1989-09-12 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
| US5288611A (en) | 1983-01-10 | 1994-02-22 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
| JPS60501339A (ja) | 1983-01-10 | 1985-08-22 | ジエン−プロ−ブ インコ−ポレィテッド | 生物を検出、同定又は定量する方法およびキット |
| US5200313A (en) | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| EP0144914A3 (en) | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridization assay employing labeled pairs of hybrid binding reagents |
| US4743535A (en) | 1984-11-07 | 1988-05-10 | Miles Inc. | Hybridization assay employing labeled probe and anti-hybrid |
| US7138505B1 (en) * | 1984-01-12 | 2006-11-21 | Novartis Vaccines And Diagnostics, Inc. | Factor VIII:C nucleic acid molecules |
| AU3844485A (en) | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
| FI71768C (fi) | 1984-02-17 | 1987-02-09 | Orion Yhtymae Oy | Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning. |
| US4512442A (en) * | 1984-03-30 | 1985-04-23 | Westinghouse Electric Corp. | Method and apparatus for improving the servicing of an elevator system |
| US6221581B1 (en) | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| CA1260372A (en) | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
| CA1253777A (en) | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Nucleic acid hybridization assay employing immobilized rna probes |
| ZA853756B (en) | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
| IL75648A0 (en) | 1984-07-05 | 1985-10-31 | Gen Probe Inc | Accelerated nucleic acid reassociation method |
| US4563417A (en) | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
| US4775619A (en) | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| US4689294A (en) | 1984-11-19 | 1987-08-25 | Miles Laboratories, Inc. | Enhancement of hybridization of nucleic acids by anionic polymers |
| IL79112A (en) | 1985-06-13 | 1992-01-15 | Abbott Lab | Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay |
| US4751177A (en) | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US5641630A (en) | 1985-06-13 | 1997-06-24 | Amgen Inc. | Method and kit for performing nucleic acid hybridization assays |
| US4710472A (en) * | 1985-09-25 | 1987-12-01 | The United States Of America As Represented By The Secretary Of The Navy | Magnetic separation device |
| US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US5750338A (en) | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US5220013A (en) * | 1986-11-17 | 1993-06-15 | Scios Nova Inc. | DNA sequence useful for the detection of Alzheimer's disease |
| AU616646B2 (en) | 1986-11-24 | 1991-11-07 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| AU601021B2 (en) | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
| IL85551A0 (en) | 1987-04-01 | 1988-08-31 | Miles Inc | Rapid hybridization assay and reagent system used therein |
| US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5656731A (en) | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
| EP0389564B1 (en) | 1987-12-01 | 1996-01-24 | Amoco Corporation | Detection of salmonella |
| US5082830A (en) | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| EP0333465B1 (en) | 1988-03-18 | 1994-07-13 | Baylor College Of Medicine | Mutation detection by competitive oligonucleotide priming |
| US6326136B1 (en) | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
| US5374524A (en) | 1988-05-10 | 1994-12-20 | E. I. Du Pont De Nemours And Company | Solution sandwich hybridization, capture and detection of amplified nucleic acids |
| NO165894C (no) | 1988-05-24 | 1991-04-24 | Gunnar Paulsen | Analysemetode for gener. |
| ATE168724T1 (de) | 1988-09-29 | 1998-08-15 | Chiron Corp | Nachweis eines polynukleotids durch ersätzung einer kette an einer fangsonde |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| CA2049043A1 (en) | 1989-03-10 | 1990-09-11 | Mark L. Collins | Immobilized oligo-nucleotide probes and uses therefor |
| US5106727A (en) | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| DE69032847T2 (de) | 1989-06-30 | 1999-05-12 | Chiron Corp., Emeryville, Calif. | Hydrophobe Nukleinsäure-Sonde |
| US5116734A (en) | 1989-09-01 | 1992-05-26 | Digene Diagnostics, Inc. | Highly sensitive method for detecting peroxidase |
| JPH05501650A (ja) | 1989-12-01 | 1993-04-02 | バイシス・インコーポレーテツド | Ｈｐｖ転写物の検出 |
| US5629153A (en) | 1990-01-10 | 1997-05-13 | Chiron Corporation | Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays |
| US5792606A (en) | 1990-02-26 | 1998-08-11 | Boehringer Mannheim Gmbh | Nucleic acid hybridization based assay for determining a substance of interest |
| CA2039517C (en) | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| US5695926A (en) | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US5484699A (en) | 1990-09-28 | 1996-01-16 | Abbott Laboratories | Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods |
| US5210704A (en) * | 1990-10-02 | 1993-05-11 | Technology International Incorporated | System for prognosis and diagnostics of failure and wearout monitoring and for prediction of life expectancy of helicopter gearboxes and other rotating equipment |
| WO1992008808A1 (en) | 1990-11-14 | 1992-05-29 | Siska Diagnostics, Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| AU672359B2 (en) | 1991-03-07 | 1996-10-03 | Virogenetics Corporation | Genetically engineered vaccine strain |
| US5283175A (en) | 1991-04-15 | 1994-02-01 | The Research Foundation Of State University Of New York | Genus-specific oligomers of Borrelia and methods of using same |
| US5556748A (en) | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
| US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
| CA2124928C (en) | 1991-12-23 | 2003-06-17 | Bruce D. Irvine | Hbv amplifier probes for use in solution phase sandwich hybridization assays |
| US5747244A (en) | 1991-12-23 | 1998-05-05 | Chiron Corporation | Nucleic acid probes immobilized on polystyrene surfaces |
| US5424413A (en) | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| FI951192L (fi) | 1992-09-15 | 1995-03-14 | Dow Chemical Co | Kestomuovien iskumodifiointi |
| DE69327454T2 (de) | 1992-10-09 | 2000-05-11 | Vysis, Inc. | Analytisches verfahren |
| US5400267A (en) * | 1992-12-08 | 1995-03-21 | Hemostatix Corporation | Local in-device memory feature for electrically powered medical equipment |
| JP3778925B2 (ja) | 1993-01-15 | 2006-05-24 | ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク | 高感度核酸サンドイッチハイブリダイゼーション検定法及びキット |
| WO1994028156A1 (en) | 1993-05-20 | 1994-12-08 | Dana-Farber Cancer Institute | Compositions and methods for treatment of herpesvirus infections |
| KR100189229B1 (ko) | 1993-07-23 | 1999-06-01 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 핵산 증폭을 강화하는 방법 |
| DE4331012A1 (de) | 1993-09-13 | 1995-03-16 | Bayer Ag | Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| DK145493D0 (da) | 1993-12-23 | 1993-12-23 | Dako As | Antistof |
| AUPM358894A0 (en) | 1994-01-31 | 1994-02-24 | Csl Limited | Modified papilloma virus l2 protein and vlps formed therefrom |
| IL112636A0 (en) | 1994-02-14 | 1995-05-26 | Macfarlane Burnet Ctre Med Res | Non-pathogenic strains of hiv-1 |
| JP3738910B2 (ja) | 1994-04-04 | 2006-01-25 | バイエル コーポレーション | 特定の核酸配列を検出するためのハイブリダイゼーション−ライゲーション分析 |
| CA2126952C (en) | 1994-06-28 | 2010-12-14 | Sithian Pandian | Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| JP3093116B2 (ja) | 1994-09-30 | 2000-10-03 | 株式会社豊田中央研究所 | 核酸検出方法 |
| US6057099A (en) | 1994-12-02 | 2000-05-02 | Intelligene Ltd. | Detection of nucleic acid sequences |
| US5747248A (en) | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US20030104361A1 (en) | 1997-09-29 | 2003-06-05 | Susan Weininger | Method of detection of nucleic acids with a specific sequence composition |
| CA2139070C (en) | 1994-12-23 | 2010-03-30 | Burton W. Blais | Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction |
| US5731153A (en) | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
| US5888724A (en) | 1995-02-17 | 1999-03-30 | The Trustees Of Columbia University In The City Of New York | Detection of high oncogenic-risk papilloma virus in high grade cervical lesions and cancers by a PCR/ELISA assay |
| US6083925A (en) | 1995-06-07 | 2000-07-04 | Connaught Laboratories Limited | Nucleic acid respiratory syncytial virus vaccines |
| EP0832280A2 (en) | 1995-06-07 | 1998-04-01 | Abbott Laboratories | Probe masking method of reducing background in an amplification reaction |
| FR2737502B1 (fr) | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
| AU726047B2 (en) | 1995-11-15 | 2000-10-26 | Gen-Probe Incorporated | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
| EP2574617B1 (en) | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6453190B1 (en) * | 1996-02-15 | 2002-09-17 | Biosense, Inc. | Medical probes with field transducers |
| US20010039007A1 (en) * | 1996-10-15 | 2001-11-08 | Robert Hallowitz | Methods and compositions for determining latent viral load |
| US5853993A (en) | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| US6117631A (en) | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| GB9624165D0 (en) | 1996-11-19 | 1997-01-08 | Amdex A S | Use of nucleic acids bound to carrier macromolecules |
| US6110676A (en) | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
| US6331181B1 (en) * | 1998-12-08 | 2001-12-18 | Intuitive Surgical, Inc. | Surgical robotic tools, data architecture, and use |
| US6132368A (en) * | 1996-12-12 | 2000-10-17 | Intuitive Surgical, Inc. | Multi-component telepresence system and method |
| US20020034737A1 (en) | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| JPH10210982A (ja) * | 1997-01-31 | 1998-08-11 | Fuji Yakuhin Kogyo Kk | 新規なタンパク質 |
| US6151039A (en) * | 1997-06-04 | 2000-11-21 | Hewlett-Packard Company | Ink level estimation using drop count and ink level sense |
| WO1998059044A1 (en) * | 1997-06-25 | 1998-12-30 | Tularik Inc. | High-throughput screening assays for modulators of primase activity |
| US6043038A (en) | 1998-03-31 | 2000-03-28 | Tularik, Inc. | High-throughput screening assays for modulators of primase activity |
| US5843995A (en) | 1997-07-07 | 1998-12-01 | University Of Medicine And Dentistry Of New Jersey | Inhibition of HIV-1 replication using oligocarbamate derivatives |
| IL121348A0 (en) * | 1997-07-21 | 1998-04-05 | Bio Rad Lab Israel Inc | System and method for device monitoring |
| AU1232099A (en) | 1997-11-04 | 1999-05-24 | Roche Diagnostics Gmbh | Specific and sensitive method for detecting nucleic acids |
| BR9814271A (pt) | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
| US6225053B1 (en) | 1997-12-12 | 2001-05-01 | Digene Corporation | Detection of hepatitis B virus |
| US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| EP1600512B1 (en) | 1997-12-22 | 2008-05-28 | Hitachi Chemical Co., Ltd. | mRNA purification on microplates |
| JP2002517981A (ja) | 1998-01-13 | 2002-06-25 | ビオヒプ テヒノロギース ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸配列を検出するための方法 |
| US6280954B1 (en) | 1998-02-02 | 2001-08-28 | Amersham Pharmacia Biotech Ab | Arrayed primer extension technique for nucleic acid analysis |
| US5994079A (en) | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
| US6686151B1 (en) | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
| US7399589B2 (en) * | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
| CA2286107C (en) * | 1998-02-10 | 2007-01-09 | Biosense, Inc. | Improved catheter calibration |
| ES2212251T3 (es) | 1998-03-23 | 2004-07-16 | Spal S.R.L. | Ventilador de flujo axial. |
| US6183956B1 (en) | 1998-03-31 | 2001-02-06 | Tularik, Incorporated | High throughput in vitro screening assays for transcription modulators |
| DE69940850D1 (de) * | 1998-08-04 | 2009-06-18 | Intuitive Surgical Inc | Gelenkvorrichtung zur Positionierung eines Manipulators für Robotik-Chirurgie |
| US20020004198A1 (en) * | 1998-12-16 | 2002-01-10 | Hardwicke Mary Ann | Novel anti-infectives |
| US20010055766A1 (en) | 1999-04-02 | 2001-12-27 | Alexander Aristarkhov | Immunosorbant assay using branched bis-biotin/avidin/multiple label complex as a detection reagent |
| WO2000060116A1 (en) | 1999-04-02 | 2000-10-12 | Tropix, Inc. | High throughput and high sensitivity detection assays |
| US7019822B1 (en) | 1999-04-29 | 2006-03-28 | Mss, Inc. | Multi-grade object sorting system and method |
| US6544732B1 (en) | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
| US6200746B1 (en) | 1999-08-25 | 2001-03-13 | Pharmacia & Upjohn Company | Methods of identifying anti-viral agents |
| US6611793B1 (en) * | 1999-09-07 | 2003-08-26 | Scimed Life Systems, Inc. | Systems and methods to identify and disable re-use single use devices based on detecting environmental changes |
| US20060275784A1 (en) | 1999-10-26 | 2006-12-07 | Ventana Medical Systems, Inc. | Detection of Human Papilloma Virus in Papanicolaou (Pap) Smears |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US6436662B1 (en) | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
| US6521190B1 (en) | 2000-05-19 | 2003-02-18 | Digene Corporation | Cell collection apparatus |
| AU2001274869A1 (en) | 2000-05-20 | 2001-12-03 | The Regents Of The University Of Michigan | Method of producing a dna library using positional amplification |
| EP1313881A2 (en) | 2000-06-06 | 2003-05-28 | TM Bioscience Corporation | Capture moieties for nucleic acids and uses thereof |
| US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| AU1548802A (en) | 2000-06-21 | 2002-01-02 | Digene Corp | Universal collection medium |
| AU2001279795A1 (en) * | 2000-08-01 | 2002-02-13 | Mirko Essling | Measuring device and measuring method for determining distance and/or position |
| AU2002226053A1 (en) | 2000-12-12 | 2002-06-24 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
| WO2002066993A1 (en) | 2001-02-16 | 2002-08-29 | Cortex Biochem, Inc. | Magnetic isolation and purification of nucleic acids |
| US6542856B2 (en) * | 2001-06-15 | 2003-04-01 | General Electric Company | System and method for monitoring gas turbine plants |
| CN103065025A (zh) * | 2001-06-20 | 2013-04-24 | 柯惠Lp公司 | 用于集成医疗跟踪的方法和系统 |
| EP1806410A3 (en) | 2001-08-23 | 2007-10-24 | Merck & Co., Inc. | Fluorescent multiplex HPV PCR assays using multiple fluorophores |
| AU2002336762A1 (en) * | 2001-09-24 | 2003-04-07 | Duke University | Fas ligand |
| LU90840B1 (en) * | 2001-09-25 | 2003-03-26 | Delphi Tech Inc | Method for controlling the operation of a system sub-system or component |
| US20050032105A1 (en) | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| DK1463839T3 (da) | 2002-01-07 | 2007-07-02 | Norchip As | Fremgangsmåde til detektering af human papillomavirus mRNA |
| JP4521152B2 (ja) * | 2002-03-05 | 2010-08-11 | 株式会社東芝 | 半導体製造装置 |
| US20030175828A1 (en) | 2002-03-15 | 2003-09-18 | Lazar James G. | Signal amplification by Hybrid Capture |
| US20030208196A1 (en) * | 2002-05-03 | 2003-11-06 | Arthrocare Corporation | Control system for limited-use device |
| US7648678B2 (en) * | 2002-12-20 | 2010-01-19 | Dako Denmark A/S | Method and system for pretreatment of tissue slides |
| EP1594950A4 (en) * | 2003-01-29 | 2007-07-25 | 454 Corp | PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS |
| ES2384170T3 (es) | 2003-04-04 | 2012-07-02 | F. Hoffmann-La Roche Ag | Sistema mejorado de PCR multicolor a tiempo real |
| US7129260B2 (en) | 2003-06-02 | 2006-10-31 | Abbott Laboratories | Isoindolinone kinase inhibitors |
| CA2531105C (en) | 2003-07-05 | 2015-03-17 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| ATE289685T1 (de) * | 2003-08-25 | 2005-03-15 | Mtm Lab Ag | Verfahren zum nachweis von karzinomen in solubilisierten zervikalen körperproben |
| WO2005033333A2 (en) | 2003-10-07 | 2005-04-14 | Dako Denmark A/S | Methods and compositions for the diagnosis of cancer |
| US8012944B2 (en) * | 2003-10-30 | 2011-09-06 | Pharmascience Inc. | Method for treating cancer using IAP antisense oligomer and chemotherapeutic agent |
| US20070083286A1 (en) * | 2004-03-09 | 2007-04-12 | Olympus Corporation | Medical instrument and equipment for managing of the same |
| CN1690223B (zh) | 2004-04-27 | 2010-05-05 | 亚能生物技术（深圳）有限公司 | 人乳头瘤病毒分型基因芯片检测系统 |
| US7398181B2 (en) * | 2004-06-02 | 2008-07-08 | Hewlett-Packard Development Company, L.P. | Method for retrieving reliability data in a system |
| US20060095096A1 (en) * | 2004-09-09 | 2006-05-04 | Debenedictis Leonard C | Interchangeable tips for medical laser treatments and methods for using same |
| ES2521115T3 (es) | 2004-09-30 | 2014-11-12 | Epigenomics Ag | Método para proveer fragmentos de ADN derivados de una muestra archivada |
| CA2585891A1 (en) | 2004-10-29 | 2006-05-11 | Medimmune, Inc. | Methods of preventing and treating rsv infections and related conditions |
| US20060129269A1 (en) * | 2004-12-15 | 2006-06-15 | Xerox Corporation | Processes for using a memory storage device in conjunction with tooling |
| AU2006205638A1 (en) | 2005-01-14 | 2006-07-20 | The Regents Of The University Of Michigan | Systems, methods, and compositions for detection of human papilloma virus in biological samples |
| US20060240449A1 (en) | 2005-01-19 | 2006-10-26 | Mcglennen Ronald C | Methods and compositions for preparation of biological samples |
| EP1928560A2 (en) | 2005-04-06 | 2008-06-11 | Verenium Corporation | Enzymes and formulations for broad-specificity decontamination of chemical and biological warfare agents |
| US7972776B2 (en) | 2005-11-15 | 2011-07-05 | Oncohealth Corporation | Protein chips for HPV detection |
| WO2007130519A2 (en) | 2006-05-02 | 2007-11-15 | Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services | Viral nucleic acid microarray and method of use |
| EP2024520B1 (en) | 2006-05-11 | 2014-03-05 | Becton, Dickinson and Company | Method of protein extraction from cells |
| ES2376978T3 (es) | 2007-02-27 | 2012-03-21 | Nuclea Biomarkers Llc | Método para predecir la respuesta de pacientes de NSCLC al tratamiento por parte de un inhibidor de EGFR-TK |
| US8357538B2 (en) | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
| US8703492B2 (en) | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
| US7985375B2 (en) | 2007-04-06 | 2011-07-26 | Qiagen Gaithersburg, Inc. | Sample preparation system and method for processing clinical specimens |
| WO2008139938A1 (ja) | 2007-05-02 | 2008-11-20 | National University Corporation Tokyo Medical And Dental University | ヒトパピローマウイルス１６型遺伝子を標的とする二本鎖核酸分子及びそれを含む医薬 |
| WO2008149237A2 (en) | 2007-06-08 | 2008-12-11 | Epigenomics Ag | Method for methylation analysis |
| CN101177701B (zh) | 2007-09-29 | 2010-08-18 | 潮州凯普生物化学有限公司 | 人乳头状瘤病毒基因分型检测试剂盒及其基因芯片制备方法 |
| WO2009049129A1 (en) | 2007-10-11 | 2009-04-16 | The Ohio State University Research Foundation | Methods and compositions for the diagnosis and treatment of esphageal adenocarcinomas |
| JP2009106220A (ja) | 2007-10-31 | 2009-05-21 | Genodive Pharma Kk | 基板上での等温増幅反応による標的塩基配列の捕捉及び検出方法 |
| HUE029729T2 (en) | 2007-11-01 | 2017-03-28 | Self-Screen B V | A new method for detecting cervical HPVs |
| WO2009123996A2 (en) | 2008-03-31 | 2009-10-08 | The Ohio State University Research Foundation | Hybridization quantitation method for modified micro-rna and -dna based oligonucleotides |
| CA2726396C (en) | 2008-04-17 | 2019-03-19 | Qiagen Gaithersburg, Inc. | Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid |
| WO2010004251A1 (en) | 2008-06-16 | 2010-01-14 | Oncomethylome Sciences Sa | Dna methylomes |
| WO2010028382A2 (en) | 2008-09-05 | 2010-03-11 | Mayo Foundation For Medical Education And Research | Collecting and processing complex macromolecular mixtures |
| US9090948B2 (en) | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
| WO2010062546A1 (en) | 2008-10-27 | 2010-06-03 | Qiagen Gaithersburg Inc. | Fast results hybrid capture assay on an automated platform |
| EP2184368A1 (en) | 2008-11-07 | 2010-05-12 | DKFZ Deutsches Krebsforschungszentrum | Diagnostic transcript and splice patterns of HPV16 in different cervical lesions |
| JP2012516155A (ja) * | 2009-01-27 | 2012-07-19 | キアゲン ゲーザーズバーグ | エンドポイント均一蛍光検出を用いた好熱性ヘリカーゼ依存性増幅技術 |
| ES2644516T3 (es) * | 2009-01-28 | 2017-11-29 | Qiagen Gaithersburg, Inc. | Método y ensayo de preparación de muestras de gran volumen específico de secuencia |
| JP5738278B2 (ja) | 2009-05-01 | 2015-06-24 | キアジェン ゲイサーズバーグ インコーポレイテッド | 試料中のｒｎａスプライシング形態を検出するための非標的増幅法 |
| WO2011028974A1 (en) * | 2009-09-03 | 2011-03-10 | Qiagen Gaithersburg, Inc. | Methods and kits for determining viral load in clinical samples |
| CA2773320C (en) * | 2009-09-14 | 2018-02-20 | Qiagen Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| BR112012016451A2 (pt) * | 2010-01-04 | 2020-09-08 | Giagen Gaithersburg, INC. | métodos, composições, e kits para recuperação de ácidos nucleicos ou proteínas de amostras de tecido fixadas |
| CA2786473A1 (en) * | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| AU2011210734B2 (en) * | 2010-01-29 | 2017-02-09 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| WO2011094528A2 (en) * | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an hpv in a sample |
| AU2011255638B2 (en) * | 2010-05-19 | 2016-08-25 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| US9376727B2 (en) * | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
-
2009
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- 2015-02-02 JP JP2015018291A patent/JP2015119719A/ja active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993010263A1 (en) * | 1991-11-14 | 1993-05-27 | Digene Diagnostics, Inc. | Non-radioactive hybridization assay and kit |
Non-Patent Citations (1)
| Title |
|---|
| Bart, "General Principles of Immunoprecipitation", 網路文獻 http://pingu.salk.edu/~sefton/Hyper_protocols/immunoprecip.html , 2004/11/03 * |
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