CA2602185A1 - Enzymes and formulations for broad-specificity decontamination of chemical and biological warfare agents - Google Patents

Enzymes and formulations for broad-specificity decontamination of chemical and biological warfare agents Download PDF

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Publication number
CA2602185A1
CA2602185A1 CA002602185A CA2602185A CA2602185A1 CA 2602185 A1 CA2602185 A1 CA 2602185A1 CA 002602185 A CA002602185 A CA 002602185A CA 2602185 A CA2602185 A CA 2602185A CA 2602185 A1 CA2602185 A1 CA 2602185A1
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Prior art keywords
seq
ala
leu
gly
val
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CA002602185A
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French (fr)
Inventor
Dan E. Robertson
Toby Richardson
Karen Kustedjo
Gabriel Amitai
Keith Lejeune
Jason Berberich
Jennifer Ann Chaplin
Jessica Sinclair
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Individual
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Life Science Research Israel Ltd
Agentase LLC
Verenium Corp
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Application filed by Life Science Research Israel Ltd, Agentase LLC, Verenium Corp filed Critical Life Science Research Israel Ltd
Publication of CA2602185A1 publication Critical patent/CA2602185A1/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02—Local antiseptics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/02—Antidotes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/02—Chemical warfare substances, e.g. cholinesterase inhibitors

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Business, Economics & Management (AREA)
  • Emergency Management (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention provides novel enzymes and methods for enzyme-based active decontamination, e.g., nerve agent detoxification. In one aspect, the invention provides enzymes having non-heme chloroperoxidase (nhCPOs) activity, and methods for bleaching and degradation of lignin. The invention provides polypeptides, nucleic acids, infective vehicles, transduced or infected cells or plants and/or transgenic plants to, and methods of using them, e.g., provide self-protecting, pesticide-resistant and/or herbicide-resistant cells or plants, where in one aspect the polypeptides of the invention act to hydrolyzing P-S or P-F
bonds and detoxify acetylcholinesterases or butyrylcholinesterases. In one aspect, the invention provides an enzyme mixture or composition that provides broad-spectrum, agent-specific oxidation and hydrolysis activities as well as hypohalite generation for oxidation or hydrolysis of V-agents, G-agents and H-agents and which generates oxidants such as chlorine dioxide and reactive radicals for killing of biological agents, including anthrax spores.

Description

ti DEMANDES OU BREVETS VOLUMINEUX
LA PRESENTE PARTIE I)E CETTE DENIANDE OU CE BREVETS
COMPREND PLUS D'UN TOME.

CECI EST LE TOME "L, DE 2 NOTE: Pour les tomes additionels, veillez contacter le Bureau Canadien des Brevets.

JUMBO APPLICATIONS / PATENTS

THIS SECTION OF THE APPLICATION / PATENT CONTAINS MORE
THAN ONE VOLUME.

THIS IS VOLUME o2 , OF ~2 NOTE: For additional volumes please contact the Canadian Patent Office.

Sequence Listing (2) SEQUENCE LISTING

<110> DIVERSA CORPORATION
AGENTASE, LLC
ROBERTSON, Dan E.
RICHARDSON, Toby CHAPLIN, Jennifer Ann KUSTEDJO, Karen AMITAI, Gabriel LEJEUNE, Keith BERBERICH, Jason SINCLAIR, Jessica <120> ENZYMES AND FORMULATIONS FOR BROAD-SPECIFICITY DECONTAMINATION OF
CHEMICAL AND BIOLOGICAL WARFARE AGENTS

<130> 564462014040 <140> Not Yet Assigned <141> Concurrently Herewith <150> 60/687,130 <151> 2005-06-02 <150> 60/669,330 <151> 2005-04-06 <160> 194 <170> PatentIn version 3.3 <210> 1 <211> 1122 <212> DNA
<213> Caldariomyces fumago <400> 1 atgttctcca aggtccttcc cttcgtggga gcggttgccg ccctccctca ctccgtccgt 60 caggagcctg gctccggcat tggctaccca tacgacaaca acaccctgcc atatgtcgcc 120 ccaggtccta ccgactctcg tgctccttgc ccagctctga acgctcttgc caaccacggt 180 tacattcctc acgatggccg tgccatcagc agggagaccc tccagaacgc tttcctcaac 240 cacatgggta ttgccaactc cgtcattgag cttgctctga ccaacgcctt cgtcgtctgc 300 gagtacgtta ctggctccga ctgtggtgac agccttgtca acctgactct gctcgccgag 360 ccccacgctt tcgagcacga ccactccttc tcccgcaagg attacaagca gggtgtcgcc 420 aactccaacg acttcatcga caacaggaac ttcgatgccg agaccttcca gacctctctg 480 gatgtcgttg caggcaagac ccacttcgac tatgccgaca tgaacgagat ccgccttcag 540 Sequence Listing (2) cgcgagtccc tctccaacga gcttgacttc cccggttggt tcaccgagtc caagccaatc 600 cagaacgtcg agtctggctt catcttcgcc cttgtctctg acttcaacct gcccgacaac 660 gatgagaacc ctctggttcg cattgactgg tggaagtact ggttcaccaa cgagtccttc 720 ccataccacc tcggctggca ccccccgtct ccagccaggg agatcgagtt cgtcacctcc 780 gcctcctccg ctgtcctggc tgcctctgtc acctctactc catcttccct tccatccggt 840 gccatcggcc caggtgccga ggctgtccct ctctccttcg cctccaccat gaccccattc 900 ctcctcgcca ccaatgctcc ttactacgcc caggacccaa ctctcggccc caacgacaag 960 cgtgaggctg ccccagctgc caccacctcc atggccgtct tcaagaaccc atacctcgag 1020 gccattggca cccaggacat caagaaccag caggcttacg tcagctccaa ggctgctgcc 1080 atggcctctg ccatggccgc caacaaggcc cgcaaccttt aa 1122 <210> 2 <211> 373 <212> PRT
<213> Caldariomyces fumago <400> 2 Met Phe Ser Lys Val Leu Pro Phe Val Gly Ala Val Ala Ala Leu Pro His Ser Val Arg Gln Glu Pro Gly Ser Gly Ile Gly Tyr Pro Tyr Asp Asn Asn Thr Leu Pro Tyr Val Ala Pro Gly Pro Thr Asp Ser Arg Ala Pro Cys Pro Ala Leu Asn Ala Leu Ala Asn His Gly Tyr Ile Pro His Asp Gly Arg Ala Ile Ser Arg Glu Thr Leu Gln Asn Ala Phe Leu Asn His Met Gly Ile Ala Asn Ser Val Ile Glu Leu Ala Leu Thr Asn Ala Phe Val Val Cys Glu Tyr Val Thr Gly Ser Asp Cys Gly Asp Ser Leu Sequence Listing (2) Val Asn Leu Thr Leu Leu Ala Glu Pro His Ala Phe Glu His Asp His Ser Phe Ser Arg Lys Asp Tyr Lys Gln Gly Val Ala Asn Ser Asn Asp Phe Ile Asp Asn Arg Asn Phe Asp Ala Glu Thr Phe Gln Thr Ser Leu Asp Val Val Ala Gly Lys Thr His Phe Asp Tyr Ala Asp Met Asn Glu Ile Arg Leu Gln Arg Glu Ser Leu Ser Asn Glu Leu Asp Phe Pro Gly Trp Phe Thr Glu Ser Lys Pro Ile Gln Asn Val Glu Ser Gly Phe Ile Phe Ala Leu Val Ser Asp Phe Asn Leu Pro Asp Asn Asp Glu Asn Pro Leu Val Arg Ile Asp Trp Trp Lys Tyr Trp Phe Thr Asn Glu Ser Phe Pro Tyr His Leu Gly Trp His Pro Pro Ser Pro Ala Arg Glu Ile Glu Phe Val Thr Ser Ala Ser Ser Ala Val Leu Ala Ala Ser Val Thr Ser Thr Pro Ser Ser Leu Pro Ser Gly Ala Ile Gly Pro Gly Ala Glu Ala Val Pro Leu Ser Phe Ala Ser Thr Met Thr Pro Phe Leu Leu Ala Thr Asn Ala Pro Tyr Tyr Ala Gln Asp Pro Thr Leu Gly Pro Asn Asp Lys Sequence Listing (2) Arg Glu Ala Ala Pro Ala Ala Thr Thr Ser Met Ala Val Phe Lys Asn Pro Tyr Leu Glu Ala Ile Gly Thr Gln Asp Ile Lys Asn Gln Gln Ala Tyr Val Ser Ser Lys Ala Ala. Ala Met Ala Ser Ala Met Ala Ala Asn Lys Ala Arg Asn Leu <210> 3 <211> 720 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 3 atggcactat catcacatct agccaagaaa cacgaattcc accctgcagg tgctggcgac 60 ttgcgaagtc cgtgccctgt gcttaacgca cttgcgaacc acggtataat atcccgcgat 120 ggccgtaata ttacggccaa tcagctgaag gacgccctca agcatattgg tgtgggtatc 180 gacatcaggc taggccttgt caattctgct tatgctgtcc atgacgagca ttcgcatcgt 240 ggccttcgca acccagaaca ggtagacgac tccggcatac ctgtcctaaa tctggatcaa 300 actggtcggc ctcatgcaat agaacacgac gtttctcttt ctagagagga tcgcgcctta 360 ggtgactgta tcagtcctca tcctggatta gttgaaggct ttctcgggta cccacagcaa 420 gacttcttca ccgtctccca tcttgggagg tttcgcaaga agcggtatat agagcaaaat 480 gcaaaaaata gggttctaga ctttggctac agggctcaca tagtggcctg cgctgaagct 540 gctctattcc agggcgtatt cggtgaaggt gtcttccgtc ggttgccggt taagtacgcg 600 aaagccgttt tccaagagga acgtttacca ttcaatgagg gctggacgcc gcggtggttt 660 gcattgatgg ttcccgagtt ggctatgtta gttctcataa tcttgtattt tgcttcctaa 720 <210> 4 <211> 239 <212> PRT

Sequence Listing (2) <213> Unknown <220>
<223> Obtained from an environmental sample <400> 4 Met Ala Leu Ser Ser His Leu Ala Lys Lys His Glu Phe His Pro Ala Gly Ala Gly Asp Leu Arg Ser Pro Cys Pro Val Leu Asn Ala Leu Ala Asn His Gly Ile Ile Ser Arg Asp Gly Arg Asn Ile Thr Ala Asn Gln Leu Lys Asp Ala Leu Lys His Ile Gly Val Gly Ile Asp Ile Arg Leu Gly Leu Val Asn Ser Ala Tyr Ala Val His Asp Glu His Ser His Arg Gly Leu Arg Asn Pro Glu Gin Val Asp Asp Ser Gly Ile Pro Val Leu Asn Leu Asp Gln Thr Gly Arg Pro His Ala Ile Glu His Asp Val Ser Leu Ser Arg Glu Asp Arg Ala Leu Gly Asp Cys Ile Ser Pro His Pro Gly Leu Val Glu Gly Phe Leu Gly Tyr Pro Gln Gln Asp Phe Phe Thr Val Ser His Leu Gly Arg Phe Arg Lys Lys Arg Tyr Ile Glu Gln Asn Ala Lys Asn Arg Val Leu Asp Phe Gly Tyr Arg Ala His Ile Val Ala Cys Ala Glu Ala Ala Leu Phe Gln Gly Val Phe Gly Glu Gly Val Phe Sequence Listing (2) Arg Arg Leu Pro Val Lys Tyr Ala Lys Ala Val Phe Gln Glu Glu Arg Leu Pro Phe Asn Glu Gly Trp Thr Pro Arg Trp Phe Ala Leu Met Val Pro Glu Leu Ala Met Leu Val. Leu Ile Ile Leu Tyr Phe Ala Ser <210> 5 <211> 729 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 5 atgtctagcc cttcgcagga actggagaaa ggcgaatatc aggctgcatc ctcaggggat 60 ttacgatctc cctgccctgc cctcaactct cttgcaaacc acggattgat tccccgcgat 120 ggtcgcaata tcaccgcaga tcagcttaaa gatgctctcc aacaaatcgg cgttggtttg 180 gatatccgac atatcctagt gaagagagca ttcactgttc atgatgagac ctctcatatc 240 ggcttccgga atccgggaca ggtaaatgag tctggaatac ctgtgttaga tctggatcag 300 actggtcgcc ctcacgctgt cgaac:acgac gtatctcttt cccgagaaga ccgcgctctg 360 ggtgactgca ttaaaccaga tccagagttg gtaagcaact tggttcagta ccccggtagc 420 aaagaatcgt tcaccagttc cgacctcggc cgattccgtc agcgccgaca tgaagagcaa 480 aaagcaaaga actctgggct ggattttgac tcatcaaaac acaaagttgc ctgtggtgaa 540 gctgctcttc ttcagtgtat ctttggcaag ggtgtcttct gcaacctccc gaccgcgtat 600 gtcaaagcaa tatttcagga ggagcgttta ccatataagg agggttggaa cccgcgatgg 660 atgccagtat tgtctccaga gctgttgatc ttacagaagg tgatatcata ttatgcttca 720 ccagcttga 729 <210> 6 <211> 242 <212> PRT
<213> Unknown Sequence Listing (2) <220>
<223> Obtained from an environmental sample.
<400> 6 Met Ser Ser Pro Ser Gln Glu Leu Glu Lys Gly Glu Tyr Gln Ala Ala Ser Ser Gly Asp Leu Arg Ser Pro Cys Pro Ala Leu Asn Ser Leu Ala Asn His Gly Leu Ile Pro Arg Asp Gly Arg Asn Ile Thr Ala Asp Gln Leu Lys Asp Ala Leu Gln Glri Ile Gly Val Gly Leu Asp Ile Arg His Ile Leu Val Lys Arg Ala Phe Thr Val His Asp Glu Thr Ser His Ile Gly Phe Arg Asn Pro Gly Glri Val Asn Glu Ser Gly Ile Pro Val Leu Asp Leu Asp Gln Thr Gly Arg Pro His Ala Val Glu His Asp Val Ser Leu Ser Arg Glu Asp Arg Ala Leu Gly Asp Cys Ile Lys Pro Asp Pro Glu Leu Val Ser Asn Leu Val. Gln Tyr Pro Gly Ser Lys Glu Ser Phe Thr Ser Ser Asp Leu Gly Arg Phe Arg Gln Arg Arg His Glu Glu Gln Lys Ala Lys Asn Ser Gly Leu Asp Phe Asp Ser Ser Lys His Lys Val Ala Cys Gly Glu Ala Ala Leu Leu Gln Cys Ile Phe Gly Lys Gly Val Phe Cys Asn Leu Pro Thr Ala Tyr Val Lys Ala Ile Phe Gln Glu Glu Sequence Listing (2) Arg Leu Pro Tyr Lys Glu Gly Trp Asn Pro Arg Trp Met Pro Val Leu Ser Pro Glu Leu Leu Ile Leu Gln Lys Val Ile Ser Tyr Tyr Ala Ser Pro Ala <210> 7 <211> 1252 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 7 cgcccttccc aagccaaagt tacactgcat ctgttatttg attgaatgca ccaataatgt 60 gtaaggtatt tgttgcccgt ggtatccagc ctcccggtgg ctcgtatata aaccctctcg 120 agatcaacat ccatctatct ggcaaaaggc ataagtcaga catctggaac taaagcagca 180 caatgaaagc cagtctactc tccgttctct ctttcttttc cctggctttg gccacagatt 240 cagccaagtt gcttccgtgg tcgcctccag ggcatggcga tggtatgtct gacttgttca 300 gttagtgatg aaaactaaga agcaacagtg cgaggcccct gcccaatgct gaataccctt 360 gcgaaccacg gacttctacc acacaatgga aaagacattt ctgaagaaga tattgtcgat 420 gtactgggca acacgctgaa tgttgcagag agccttgctg ttgctctgta taaagaggcc 480 atgaccacag ttgaagaccc caacgcgact accttctcct tgagtgacct tgatcgccct 540 ggtatcctag agcatgatgc cagtttgagg tatctgcaac cccagataca tcaggaaatc 600 aagctgactc gcccagccgc caggaccctt acttcgggaa tgatcatgag ttcaaccaga 660 aaatattcga ccagacgaag tcgtactgga caagtcctct gattgatatt gaagaagcag 720 cagaagcaca tgaagcccgg tggaacactt ccaaggccac taatcctgac tttaatgtct 780 ctgacaccgg gacagcattc agttt.cggag agtctgccgc ctacatgatc gcatttgaag 840 aagacggtct tggatatgcc aacagatctt gggtagtata cttttttggt gggtgggctt 900 Sequence Listing (2) gatgaattat gagtcaatag cgccgctaac atgttaaatg cgtagaaaac gaacgcctgc 960 cccaagagct tggatggact aagcgtccat ttgtcactac aagcgaaact ctgttcaaca 1020 tgacccagcg gattgtgaat tccactgttg gtctcacacc agaagagttt gcccagctgc 1080 aagactttgc taaagcgaac cccagcttcg ctgggtcttc gtaaaatcct ggtattgaag 1140 ggttttcctg tcatctagcg ctgggggatt atcatgcccg agaaaaagaa agttgcatag 1200 tggaacaaga tttgtgtttt gatggatacg gcaggtaatg cgaagggcga at 1252 <210> 8 <211> 263 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 8 Met Lys Ala Ser Leu Leu Ser Val Leu Ser Phe Phe Ser Leu Ala Leu Ala Thr Asp Ser Ala Lys Leu Leu Pro Trp Ser Pro Pro Gly His Gly Asp Val Arg Gly Pro Cys Pro Met Leu Asn Thr Leu Ala Asn His Gly Leu Leu Pro His Asn Gly Lys Asp Ile Ser Glu Glu Asp Ile Val Asp Val Leu Gly Asn Thr Leu Asn Val Ala Glu Ser Leu Ala Val Ala Leu Tyr Lys Glu Ala Met Thr Thr Val Glu Asp Pro Asn Ala Thr Thr Phe Ser Leu Ser Asp Leu Asp Arg Pro Gly Ile Leu Glu His Asp Ala Ser Leu Ser Arg Gln Asp Pro Tyr Phe Gly Asn Asp His Glu Phe Asn Gln Sequence Listing (2) Lys Ile Phe Asp Gln Thr Lys Ser Tyr Trp Thr Ser Pro Leu Ile Asp Ile Glu Glu Ala Ala Glu Ala His Glu Ala Arg Trp Asn Thr Ser Lys Ala Thr Asn Pro Asp Phe Asn Val Ser Asp Thr Gly Thr Ala Phe Ser Phe Gly Glu Ser Ala Ala Tyr Met Ile Ala Phe Glu Glu Asp Gly Leu Gly Tyr Ala Asn Arg Ser Trp Val Val Tyr Phe Phe Glu Asn Glu Arg Leu Pro Gln Glu Leu Gly Trp Thr Lys Arg Pro Phe Val Thr Thr Ser Glu Thr Leu Phe Asn Met Thr Gln Arg Ile Val Asn Ser Thr Val Gly Leu Thr Pro Glu Glu Phe Ala Gln Leu Gln Asp Phe Ala Lys Ala Asn Pro Ser Phe Ala Gly Ser Ser <210> 9 <211> 1172 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 9 cccttcgtaa ttccttccag atgacgctgg caagtaatat tccggcattt gtaatgtaga 60 tggctgagct ctaggcctac atccgaatat aaaatctatc gacccctcat cctgaataca 120 gtcccagaat aacaaattta gctctctatt acaactaccg ccatcatgaa gacagcaacc 180 ctactatttc tcgcggcagg tcttacccaa acgcaagcat tcccatcaca aggagcagca 240 Sequence Listing (2) ccgcatccac taccatggtc ccctcctggc cctaacgatg gtaagcaccc tatttagcct 300 acccacataa tcaaacaact aacagaaccg aaagtacgcg ccccctgccc aatgctcaac 360 accctcgcca accacggcta cctcccgcac aacggcaaaa acatcaccga gcaacacaca 420 atcaacgccc tctacaacgc cctcggcatc gacgcagaac tctccgcatt cctccaccaa 480 gaagcagtaa ccaccaaccc aacccccaac gctactacct tctccttgaa tgatctaagc 540 cgtcatgata tcctcgagca tgacgccagc ttgaggtatg tccctcccca atcccaccac 600 tccatccatc taacccagta tgtattatgg tatagccgcc aagacgccta cttcggcgac 660 aaccacgact tcaatcaaac catcttcgac gaaacccgct catactggac ctcccctatc 720 atcgacgtga agcaggctgc tctgtctcgc caagcacgtg tgaatacctc tatggctacg 780 aatccgaatt ataccatgtc cgagctgggt gcgtcgttca gttatgggga gacggcggcg 840 tatattattg ttcttgggga taaggagaat gggttggtga atcggtcgag ggtggagtat 900 ctttttggtg agttagaccc ttcgttgggt tgaagtagtg gtggagagtt catggttggc 960 taatgtattg cagagaatga acgtcttccg ctggatttag ggtggactcg tgcgaaggag 1020 aatatcacct ttgatgattt gagaacgatg ttgaatagga ttgttaatgc caccggtggg 1080 gagagtgagt ttgataggga gttggcgaag aggggtgggg tgcatgttgg gaggtggagg 1140 gggtactagg tggactttgt tctgggtaag gg 1172 <210> 10 <211> 267 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 10 Met Lys Thr Ala Thr Leu Leu Phe Leu Ala Ala Gly Leu Thr Gln Thr Gln Ala Phe Pro Ser Gln Gly Ala Ala Pro His Pro Leu Pro Trp Ser Pro Pro Gly Pro Asn Asp Val Arg Ala Pro Cys Pro Met Leu Asn Thr Sequence Listing (2) Leu Ala Asn His Gly Tyr Leu Pro His Asn Gly Lys Asn Ile Thr Glu Gln His Thr Ile Asn Ala Leu Tyr Asn Ala Leu Gly Ile Asp Ala Glu Leu Ser Ala Phe Leu His Gln Glu Ala Val Thr Thr Asn Pro Thr Pro Asn Ala Thr Thr Leu Ser Leu Asn Asp Leu Ser Arg His Asp Ile Leu Glu His Asp Ala Ser Leu Ser Arg Gln Asp Ala Tyr Phe Gly Asp Asn His Asp Phe Asn Gln Thr Ile Phe Asp Glu Thr Arg Ser Tyr Trp Thr Ser Pro Ile Ile Asp Val Lys Gln Ala Ala Leu Ser Arg Gln Ala Arg Val Asn Thr Ser Met Ala Thr Asn Pro Asn Tyr Thr Met Ser Glu Leu Gly Ala Ser Phe Ser Tyr Gly Glu Thr Ala Ala Tyr Ile Ile Val Leu Gly Asp Lys Glu Asn Gly Leu Val Asn Arg Ser Arg Val Glu Tyr Leu Phe Glu Asn Glu Arg Leu Pro Leu Asp Leu Gly Trp Thr Arg Ala Lys Glu Asn Ile Thr Phe Asp Asp Leu Arg Thr Met Leu Asn Arg Ile Val Asn Ala Thr Gly Gly Glu Ser Glu Phe Asp Arg Glu Leu Ala Lys Arg Gly Gly Val His Val Gly Arg Trp Arg Gly Tyr Sequence Listing (2) <210> 11 <211> 1169 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 11 ttcgtaattc cttccagatg acgctggcaa gtaatattcc ggcatttgta atgtagatgg 60 ctgagctcta ggcctacatc cgaatataaa atctatcgac ccctcatcct gaatacagtc 120 ccagaataac aaatttagct ctctattaca actaccgcca tcatgaagac agcaacccta 180 ctatttctcg cggcaggtct tacccaaacg caagcattcc catcacaagg agcagcaccg 240 catccactac catggtcccc tcctggccct aacgatggta agcaccctat ttagcctacc 300 cacataatca aacaactaac agaaccgaaa gtacgcgccc cctgcccaat gctcaacacc 360 ctcgccaacc acggctacct cccgcacaac ggcaaaaaca tcaccgagca acacacaatc 420 aacgccctct acaacgccct cggcatcgac gcagaactct ccgcattcct ccaccaagaa 480 gcagtaacca ccaacccaac ccccaacgct actaccttct ccttgaatga tctaagccgt 540 catgatatcc tcgagcatga cgccagcttg aggtatgtcc ctccccaatc ccaccactcc 600 atccatctaa cccagtatgt attatggtat agccgccaag acgcctactt cggcgacaac 660 cacgacttca atcaaaccat cttcgacgaa acccgctcat actggacctc ccctatcatc 720 gacgtgaagc aggctgctct gtctcgccaa gcacgtgtga atacctctat ggctacgaat 780 ccgaattata ccatgtccga gctgggtgcg tcgttcagtt atggggagac ggcggcgtat 840 attattgttc ttggggataa ggagaatggg ttggtgaatc ggtcgagggt ggagtatctt 900 tttggtgagt tagacccttc gttgggttga agtagtggtg gagagttcat ggttggctaa 960 tgtattgcag agaatgaacg tcttccgctg gatttagggt ggactcgtgc gaaggagaat 1020 atcacctttg atgatttgag aacgatgttg aataggattg ttaatgccac cggtggggag 1080 agtgagtttg atagggagtt ggcgaagagg ggtggggtgc atgttgggag gtggaggggg 1140 tactaggtgg actttgttct gggtaaggg 1169 <210> 12 Sequence Listing (2) <211> 267 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 12 Met Lys Thr Ala Thr Leu Leu Phe Leu Ala Ala Gly Leu Thr Gln Thr Gln Ala Phe Pro Ser Gln Gly Ala Ala Pro His Pro Leu Pro Trp Ser Pro Pro Gly Pro Asn Asp Val Arg Ala Pro Cys Pro Met Leu Asn Thr Leu Ala Asn His Gly Tyr Leu Pro His Asn Gly Lys Asn Ile Thr Glu Gln His Thr Ile Asn Ala Leu Tyr Asn Ala Leu Gly Ile Asp Ala Glu Leu Ser Ala Phe Leu His Gln Glu Ala Val Thr Thr Asn Pro Thr Pro Asn Ala Thr Thr Phe Ser Leu. Asn Asp Leu Ser Arg His Asp Ile Leu Glu His Asp Ala Ser Leu Ser Arg Gln Asp Ala Tyr Phe Gly Asp Asn His Asp Phe Asn Gln Thr Ile Phe Asp Glu Thr Arg Ser Tyr Trp Thr Ser Pro Ile Ile Asp Val Lys Gln Ala Ala Leu Ser Arg Gln Ala Arg Val Asn Thr Ser Met Ala Thr Asn Pro Asn Tyr Thr Met Ser Glu Leu Gly Ala Ser Phe Ser Tyr Gly Glu Thr Ala Ala Tyr Ile Ile Val Leu Sequence Listing (2) Gly Asp Lys Glu Asn Gly Leu Val Asn Arg Ser Arg Val Glu Tyr Leu Phe Glu Asn Glu Arg Leu Pro Leu Asp Leu Gly Trp Thr Arg Ala Lys Glu Asn Ile Thr Phe Asp Asp Leu Arg Thr Met Leu Asn Arg Ile Val Asn Ala Thr Gly Gly Glu Ser Glu Phe Asp Arg Glu Leu Ala Lys Arg Gly Gly Val His Val Gly Arg Trp Arg Gly Tyr <210> 13 <211> 928 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 13 atgatgaagc tcactgctgt tcttgccgcc tgcacttccg cgctcgcttc tgcgaggccc 60 cagtatggtg ccgcaactcc agatccacac gcttggatgc ctgcctccac aaacgactgt 120 aagtgtacac accaaaaaga ccatgctatg gttcagagct gatacgcgca gtccgcggcc 180 catgcccgat gctcaacact ctagcaaatc acggtttcct tcctcgcgat ggccgcaact 240 tcacccagga gaacgtcgtt aagggcctga aggacggctt gaactttaat ggttcacttg 300 gcgcactcat gtggcagcaa gccatcatcg caaaccctga accaaacgct acgttcttca 360 ctctagagca gctcaacgtc cacaatgttc ttgagcacga tgccagtctt acccgtactg 420 atgctgcctt cggtaacaac catgtcttca atgagactgt atacaacaca tcaagaaagt 480 ggtggacaga tgagaccgta acacctgaaa tgctggccaa ctccaagata tttcgccagt 540 tggagagccg tgcaaccaac ccaaactaca cctttactgc ttccaccgag gagtttagtt 600 tgggtgaagt ctcggcacct atcattgcct ttggcagatt agaagacggt accgtcaacc 660 Sequence Listing (2) gcacgttgat ggagtacttc ttcggtaagt tctttggcaa cgcgcaatcg tgtgcgagga 720 gcggtggtgc agtaactgac aactgggcag agaacgagcg tcttcctttt gagttgggct 780 ggactcagaa gacggacgag atcaccctca tgaagatcat ggctactaca gacatcattc 840 gcaacgcgac gagtctactt actggtggcg aaaccgctga ggattctcct catggcaaac 900 gcgatctcca tggtggaatg ttctagag 928 <210> 14 <211> 290 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 14 Met Met Lys Leu Thr Ala Val Leu Ala Ala Cys Thr Ser Ala Leu Ala Ser Ala Arg Pro Gln Tyr Gly Ala Ala Thr Pro Asp Pro His Ala Trp Met Pro Ala Ser Thr Asn Asp Phe Arg Gly Pro Cys Pro Met Leu Asn Thr Leu Ala Asn His Gly Phe Leu Pro Arg Asp Gly Arg Asn Phe Thr Gln Glu Asn Val Val Lys Gly Leu Lys Asp Gly Leu Asn Phe Asn Gly Ser Leu Gly Ala Leu Met Trp Gln Gln Ala Ile Ile Ala Asn Pro Glu Pro Asn Ala Thr Phe Phe Thr Leu Glu Gln Leu Asn Val His Asn Val Leu Glu His Asp Ala Ser Leu Thr Arg Thr Asp Ala Ala Phe Gly Asn Asn His Val Phe Asn Glu Thr Val Tyr Asn Thr Ser Arg Lys Trp Trp Sequence Listing (2) Thr Asp Glu Thr Val Thr Pro Glu Met Leu Ala Asn Ser Lys Ile Phe Arg Gln Leu Glu Ser Arg Ala Thr Asn Pro Asn Tyr Thr Phe Thr Ala Ser Thr Glu Glu Phe Ser Leu Gly Glu Val Ser Ala Pro Ile Ile Ala Phe Gly Arg Leu Glu Asp Gly Thr Val Asn Arg Thr Leu Met Glu Tyr Phe Phe Gly Lys Phe Phe Gly Asn Ala Gln Ser Cys Ala Arg Ser Gly Gly Ala Val Thr Asp Asn Trp Ala Glu Asn Glu Arg Leu Pro Phe Glu Leu Gly Trp Thr Gln Lys Thr Asp Glu Ile Thr Leu Met Lys Ile Met Ala Thr Thr Asp Ile Ile Arg Asn Ala Thr Ser Leu Leu Thr Gly Gly Glu Thr Ala Glu Asp Ser Pro His Gly Lys Arg Asp Leu His Gly Gly Met Phe <210> 15 <211> 756 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 15 atgcatctgc cattcctcaa gacaattctc ttcaccctcg taaccagctg caatgcttgg 60 Sequence Listing (2) tcccccgaag gccacgactg gcatccaccc tactcaaccg acagccgctc gccatgccca 120 ggtctcaacg cactagccaa ccacggctac ctcccacgat ccgggaaaga aatcgaccta 180 gcaactctac gcaacgcagt ctccaaagcc tacaactacc tcccaacggc gttcgacgaa 240 gccttcgcgc aagcagtctc cttcaacctc acaacaacag gcaacgaatc aaccttcaac 300 ctggtcgacc tagcccgcca cgacgatatc gaattcgacg gctccctctc ccgaaacgac 360 atctacttcg gcgacaatct gcacttcgac cccaccatct ggaaatccgt cgctgaccac 420 ctcgatctct atgacaccct gggcgcagag agcaagcagt atgtcactgt tgaaatggcg 480 gcccgcgcgc gcgctgcccg tgtgctcgat gctaagagca ggaatcccga gttcaatgct 540 tccgatgcgg aggctattgg gagtcccggg accacgggcc tttatttgac gaccctgtgg 600 gatgatcggg ttggggctgc gcctaaggct tggattcggg ctttcttcga ggaagaacgt 660 attcccttcc tagagggctt tagaggtccc aaagtcgcac ggactagtga tgatttcgct 720 gagatgacta aacgcgtgct tgctgtcgat gtttag 756 <210> 16 <211> 251 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 16 Met His Leu Pro Phe Leu Lys Thr Ile Leu Phe Thr Leu Val Thr Ser Cys Asn Ala Trp Ser Pro Glu Gly His Asp Trp His Pro Pro Tyr Ser Thr Asp Ser Arg Ser Pro Cys Pro Gly Leu Asn Ala Leu Ala Asn His Gly Tyr Leu Pro Arg Ser Gly Lys Glu Ile Asp Leu Ala Thr Leu Arg Asn Ala Val Ser Lys Ala Tyr Asn Tyr Leu Pro Thr Ala Phe Asp Glu Sequence Listing (2) Ala Phe Ala Gln Ala Val Ser Phe Asn Leu Thr Thr Thr Gly Asn Glu Ser Thr Phe Asn Leu Val Asp Leu Ala Arg His Asp Asp Ile Glu Phe Asp Gly Ser Leu Ser Arg Asn Asp Ile Tyr Phe Gly Asp Asn Leu His Phe Asp Pro Thr Ile Trp Lys Ser Val Ala Asp His Leu Asp Leu Tyr Asp Thr Leu Gly Ala Glu Ser Lys Gin Tyr Val Thr Val Glu Met Ala Ala Arg Ala Arg Ala Ala Arg Val Leu Asp Ala Lys Ser Arg Asn Pro Glu Phe Asn Ala Ser Asp Ala Glu Ala Ile Gly Ser Pro Gly Thr Thr Gly Leu Tyr Leu Thr Thr Leu Trp Asp Asp Arg Val Gly Ala Ala Pro Lys Ala Trp Ile Arg Ala Phe Phe Glu Glu Glu Arg Ile Pro Phe Leu Glu Gly Phe Arg Gly Pro Lys Val Ala Arg Thr Ser Asp Asp Phe Ala Glu Met Thr Lys Arg Val Leu Ala Val Asp Val <210> 17 <211> 1107 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 17 Sequence Listing (2) tcgccttgtt tgagctaacc caatatggaa catttgaaga agcaaagtat ttgatatcgg 60 cttcgagatg atgtataagt acgccacgag ttacggttgg gaagaaaaga ataagacaga 120 gtagctctac cattagtctc aattacactg ggaaactagc ataatacttc aatcaaccaa 180 catgcgtggc ttgtttttca tctcgactct ggggagcttg gccgccggct ttgctctgcc 240 caactttcct gtaaagggcc atgaatggat tccaggagga ccagaagata gtgagtttac 300 agaaacgact cctccataca caggaaactg acagtgcatt ttcaggtcga tctccttgcc 360 ctggcatcaa cgtccttgct aaccacggct accttccacg caatggcagg aacatcgatc 420 tgccaactct tcaaaacgcc gtcgctgctg catacaacta tgctcccaac acttttgacg 480 atgtctttgc gcaggttcaa agcatgcagc tctcaaccac gggaaacatt tccaccttca 540 atctccatga cgtgaccaag catggttaca tggagtttga cggatcgttg tcgcgcaatg 600 acgtttactt cggaaatgcc gagcgctttg atcccaagat ttggaaaacc atgtctgaca 660 gaatggcttt gaataaggtc acgagggatc ccatgtccaa gtatgtgact gtggaaacgg 720 cggccaaggc gagggctgcg cgcgttgcag acgcgatgaa ggccaacccg accttcaatg 780 cttcggctct tgagatgcag ggcagccccg gcacgacggc tctgtatttg accacgctgt 840 gggatgattc tgttggagct gctcctaagg aatggatcaa gatgttcttt ggtaagtcga 900 cctgctggct gattgctata tactatctgc taggactacg ctgatacttt agcagagttg 960 gaacgcctgc cttttactaa gccaaagacc cagaagacta atatggatat cggaaacatg 1020 tttgcaaagg tctcggccgt gcaagtctaa gtttaaacaa tcagtacgca ttggattgct 1080 cataatggca ttcttgaaag ccggagt 1107 <210> 18 <211> 250 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 18 Met Arg Gly Leu Phe Phe Ile Ser Thr Leu Gly Ser Leu Ala Ala Gly Phe Ala Leu Pro Asn Phe Pro Val Lys Gly His Glu Trp Ile Pro Gly Sequence Listing (2) Gly Pro Glu Asp Ser Arg Ser Pro Cys Pro Gly Ile Asn Val Leu Ala Asn His Gly Tyr Leu Pro Arg Asn Gly Arg Asn Ile Asp Leu Pro Thr Leu Gln Asn Ala Val Ala Ala Ala Tyr Asn Tyr Ala Pro Asn Thr Phe Asp Asp Val Phe Ala Gin Val Gln Ser Met Gln Leu Ser Thr Thr Gly Asn Ile Ser Thr Phe Asn Leu His Asp Val Thr Lys His Gly Tyr Met Glu Phe Asp Gly Ser Leu Ser Arg Asn Asp Val Tyr Phe Gly Asn Ala Glu Arg Phe Asp Pro Lys Ile Trp Lys Thr Met Ser Asp Arg Met Ala Leu Asn Lys Val Thr Arg Asp Pro Met Ser Lys Tyr Val Thr Val Glu Thr Ala Ala Lys Ala Arg Ala Ala Arg Val Ala Asp Ala Met Lys Ala Asn Pro Thr Phe Asn Ala Ser Ala Leu Glu Met Gln Gly Ser Pro Gly Thr Thr Ala Leu Tyr Leu Thr Thr Leu Trp Asp Asp Ser Val Gly Ala Ala Pro Lys Glu Trp Ile Lys Met Phe Phe Ala Glu Leu Glu Arg Leu Pro Phe Thr Lys Pro Lys Thr Gln Lys Thr Asn Met Asp Ile Gly Asn Sequence Listing (2) Met Phe Ala Lys Val Ser Ala Val Gin Val <210> 19 <211> 1253 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 19 cgcccttgtg ggctgtggac ttgcatctcg tttccgctca agacattgtt gttatctggg 60 tgaaaagaaa atatacgctc gttcttcaaa atgcgttcta cattcgccgt ctcactgctt 120 gctggagttg ttgtatcagc agaaggcctc ctagatcttc cgctcgacat tgccggttta 180 ctctcttcgc tcagcccagc acctccaacg gaccctcggt ggacagactg gcatccagca 240 ggaagcagcg atggtacatg cacccctgct atccacattc tccacccaag tcactaatca 300 ttctgacccc caacagtacg ctccccatgc ccagccctaa acagcctcgc aaaccacggc 360 ttcatccacc gcgacggccg caacatgacc atcccgcacc tcatctccgg cctcgctgca 420 ggtctcaaca taggcgccga cttcactact gccatcagcg gcgccggcct gctctcctct 480 cccaatccgc tcgggggctc cttcgacctc gatgacttga gccagcataa cttccccatt 540 gagcacgacg ccagtatgtc gcgtcaagat gccgcattgg gtaacccaca gccattttac 600 aatccaaact ggcagcagta catttctttt ttcgctggca aggaccagac ggatgtgcct 660 acagcagcca aggcaaagta cgccaggacg caagatagtc agaagcggaa tccagaattc 720 gtgtatgggt tgcgagaagc ggtgtttagt tatggcgaga atgcgcttta tttgcagact 780 atggggtctg atccgttgtc gggaaaagcc aaggtagagt atgtgaggag cttgtttgag 840 caggaaaagt tgccgtttga gcttgggtgg aggccgagtg tgctgcctat cacgctgcct 900 agtttgggag ccatggttgt ggagctgcag gcgagtagcc cagagcctct gcccgagggc 960 gtgaagattg tggcggactc gtacaaggat gtttttattg ctgtggctgg tgggtcgaat 1020 atcctgggga atcttactgc ggggattagt gaggcgttgg gtttgtgaga gcatacgggg 1080 caagacatag gcttgtgttg atggtatcag ggggggacgt gttgagcttc catcgttatt 1140 attaccctgt ttctagtcta atttggttat cgccatgttc gaacttcgtc tccatttctc 1200 Sequence Listing (2) aagatcatca acccttattc ctcgactcta gacagtcgca catgcgaagg gcg 1253 <210> 20 <211> 304 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<220>
<221> MISCFEATURE
<222> (98)..(98) <223> Amino acid in position 98 can be "A" or "T"
<400> 20 Met Arg Ser Thr Phe Ala Val Ser Leu Leu Ala Gly Val Val Val Ser Ala Glu Gly Leu Leu Asp Leu Pro Leu Asp Ile Ala Gly Leu Leu Ser Ser Leu Ser Pro Ala Pro Pro Thr Asp Pro Arg Trp Thr Asp Trp His Pro Ala Gly Ser Ser Asp Val. Arg Ser Pro Cys Pro Ala Leu Asn Ser Leu Ala Asn His Gly Phe Ile His Arg Asp Gly Arg Asn Met Thr Ile Pro His Leu Ile Ser Gly Leu Ala Ala Gly Leu Asn Ile Gly Ala Asp Phe Ala Thr Ala Ile Ser Gly Ala Gly Leu Leu Ser Ser Pro Asn Pro Leu Gly Gly Ser Phe Asp Leu Asp Asp Leu Ser Gln His Asn Phe Pro Ile Glu His Asp Ala Ser Met: Ser Arg Gln Asp Ala Ala Leu Gly Asn Sequence Listing (2) Pro Gln Pro Phe Tyr Asn Pro Asn Trp Gln Gln Tyr Ile Ser Phe Phe Ala Gly Lys Asp Gln Thr Asp Val Pro Thr Ala Ala Lys Ala Lys Tyr Ala Arg Thr Gln Asp Ser Gln Lys Arg Asn Pro Glu Phe Val Tyr Gly Leu Arg Glu Ala Val Phe Ser Tyr Gly Glu Asn Ala Leu Tyr Leu Gln Thr Met Gly Ser Asp Pro Leu. Ser Gly Lys Ala Lys Val Glu Tyr Val Arg Ser Leu Phe Glu Gln Glu Lys Leu Pro Phe Glu Leu Gly Trp Arg Pro Ser Val Leu Pro Ile Thr Leu Pro Ser Leu Gly Ala Met Val Val Glu Leu Gln Ala Ser Ser Prc> Glu Pro Leu Pro Glu Gly Val Lys Ile Val Ala Asp Ser Tyr Lys Asp Val Phe Ile Ala Val Ala Gly Gly Ser Asn Ile Leu Gly Asn Leu Thr Ala Gly Ile Ser Glu Ala Leu Gly Leu <210> 21 <211> 906 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 21 atgaagacct cattcatcag cctggcattc gttgccaccg tcgttagtgg ccaaggactg 60 gacctgggat cgctgctcac tggtggtgct gcacccgact cagacccaag atggctcaac 120 Sequence Listing (2) tggaagccag ctggatctgg tgactcgagg tctccttgcc caggacttaa ttctcttgcc 180 aaccacggct ttttgaacca caatggcaag gacattacaa tcccacagct tgttcagggt 240 ggacgtgaag ggctcaacat gggagccgac ttcatggcaa tcattggagc gattggtctt 300 cagtcttcgc caaacccgct tgatggaaag tttgatcttt ccgatcttga ccagcacaac 360 ttccctattg agcacgatgg ctcgctgtct cgtgaagacg acttcttcgg cgacaacacc 420 aacttcaacc agactgtgtt cgatcaattc ctctccaact tccacggagc tgccaagacc 480 aacaccgaca ccgcgtcgaa ggcccgatac tctcgaatcc aagacagctt ggaccgcaac 540 ccaaccgtca cctatggcgc tctccagttc cttctcagca gtggagagac tgggctttat 600 gttcagacca tgagcagccc taccgagaac gcagcacgcc tggactatgt gaagagcttg 660 tttgagcagg agagacttcc tttcaacatt ggatggagag ctagcaagtc tcagatcact 720 ctcacaaccc tcggaaccta cattcttcag ctcttcgctg cgaacccaga caacgccgct 780 gagggcctca cccttacggt cggaactttg ggaagcactc tcgcgactct tgcgggtgga 840 gcgaatgtgc tgtcgaacat cactgagggt attaccgacg ccttggactt gcctttcctc 900 cagtaa 906 <210> 22 <211> 301 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 22 Met Lys Thr Ser Phe Ile Ser Leu Ala Phe Val Ala Thr Val Val Ser Gly Gln Gly Leu Asp Leu Gly Ser Leu Leu Thr Gly Gly Ala Ala Pro Asp Ser Asp Pro Arg Trp Leu Asn Trp Lys Pro Ala Gly Ser Gly Asp Ser Arg Ser Pro Cys Pro Gly Leu Asn Ser Leu Ala Asn His Gly Phe Sequence Listing (2) Leu Asn His Asn Gly Lys Asp Ile Thr Ile Pro Gln Leu Val Gln Gly Gly Arg Glu Gly Leu Asn Met Gly Ala Asp Phe Met Ala Ile Ile Gly Ala Ile Gly Leu Gln Ser Ser Pro Asn Pro Leu Asp Gly Lys Phe Asp Leu Ser Asp Leu Asp Gln His Asn Phe Pro Ile Glu His Asp Gly Ser Leu Ser Arg Glu Asp Asp Phe Phe Gly Asp Asn Thr Asn Phe Asn Gln Thr Val Phe Asp Gln Phe Leu Ser Asn Phe His Gly Ala Ala Lys Thr Asn Thr Asp Thr Ala Ser Lys Ala Arg Tyr Ser Arg Ile Gin Asp Ser Leu Asp Arg Asn Pro Thr Val. Thr Tyr Gly Ala Leu Gln Phe Leu Leu Ser Ser Gly Glu Thr Gly Leu Tyr Val Gln Thr Met Ser Ser Pro Thr Glu Asn Ala Ala Arg Leu Asp Tyr Val Lys Ser Leu Phe Glu Gln Glu Arg Leu Pro Phe Asn Ile Gly Trp Arg Ala Ser Lys Ser Gln Ile Thr Leu Thr Thr Leu Gly Thr Tyr Ile Leu Gln Leu Phe Ala Ala Asn Pro Asp Asn Ala Ala Glu Gly Leu Thr Leu Thr Val Gly Thr Leu Gly Ser Thr Leu Ala Thr Leu Ala Gly Gly Ala Asn Val Leu Ser Asn Ile Thr Sequence Listing (2) Glu Gly Ile Thr Asp Ala Leu Asp Leu Pro Phe Leu Gln <210> 23 <211> 831 <212> DNA
<213> Botrytis cineria <400> 23 atgtttgcaa atattatttt gctagccgct gttttgcccg tttttttagc tcacgctcaa 60 ggttctttta ctacctggtc tcccccagga cctggcgatg tacgttctcc ttgtccggca 120 atgaacagtc tagctaatca tggttttctt ccgcatgacg gtaaaggcat cactgtacct 180 gatgtaataa ctgcgttgga cgccgcttta aacgttgggg ctgatttcgc gacagccatt 240 ggatcagcag gtctccttag cgttcaaggg aatcttcttg ctacttcatt caacttggat 300 gatttgaatg aacataattt tcctattgaa cacgatggat ctctttctcg cgctgattat 360 tatagctcac caaatcatga cgattattcg ttcaaccaga caattttcga tcaatttcta 420 agttttttta catcccctga gacctccata cctgttgctg cagctgctaa atatgcacgt 480 gtaaagtacg aagaggaaca tgatccacat ttttcctatt cagcccaaca ttttgtctta 540 tcttacggtg agactgcact gtatttaagt accttaggag accctgtcga aggagtggcc 600 gacgtggaat gggtccgagt cttttttgag gaagaacgtt tgccatacag ggaaggttgg 660 agaagaggtt cggttcagac aaacttaccc tctctcgctg gtatgatcgc tcggcttatt 720 cttgcgaatg aagatgtttt acctgaaggc ttagaggtta cggcacaaac tgtgggcata 780 gcattcggtg gatataatcc agctacggga gttttgaatc acgttatcgg t 831 <210> 24 <211> 277 <212> PRT
<213> Botrytis cineria <400> 24 Met Phe Ala Asn Ile Ile Leu. Leu Ala Ala Val Leu Pro Val Phe Leu Ala His Ala Gln Gly Ser Phe Thr Thr Trp Ser Pro Pro Gly Pro Gly Sequence Listing (2) Asp Val Arg Ser Pro Cys Pro Ala Met Asn Ser Leu Ala Asn His Gly Phe Leu Pro His Asp Gly Lys Gly Ile Thr Val Pro Asp Val Ile Thr Ala Leu Asp Ala Ala Leu Asn Val Gly Ala Asp Phe Ala Thr Ala Ile Gly Ser Ala Gly Leu Leu Ser Val Gln Gly Asn Leu Leu Ala Thr Ser Phe Asn Leu Asp Asp Leu Asn Glu His Asn Phe Pro Ile Glu His Asp Gly Ser Leu Ser Arg Ala Asp Tyr Tyr Ser Ser Pro Asn His Asp Asp Tyr Ser Phe Asn Gln Thr Ile Phe Asp Gln Phe Leu Ser Phe Phe Thr Ser Pro Glu Thr Ser Ile Pro Val Ala Ala Ala Ala Lys Tyr Ala Arg Val Lys Tyr Glu Glu Glu His Asp Pro His Phe Ser Tyr Ser Ala Gln His Phe Val Leu Ser Tyr Gly Glu Thr Ala Leu Tyr Leu Ser Thr Leu Gly Asp Pro Val Glu Gly Val Ala Asp Val Glu Trp Val Arg Val Phe Phe Glu Glu Glu Arg Leu Pro Tyr Arg Glu Gly Trp Arg Arg Gly Ser Val Gln Thr Asn Leu Pro Ser Leu Ala Gly Met Ile Ala Arg Leu Ile Sequence Listing (2) Leu Ala Asn Glu Asp Val Leu Pro Glu Gly Leu Glu Val Thr Ala Gln Thr Val Gly Ile Ala Phe Gly Gly Tyr Asn Pro Ala Thr Gly Val Leu Asn His Val Ile Gly <210> 25 <211> 708 <212> DNA
<213> Fusarium verticillicides <400> 25 gatccaaaag gtcatgaatt tagagccgcg ggtccctatg attcccgttc accttgtcct 60 ggacttaatg ctcttgcaaa tcatggatat ttgccccgtg atggctctaa cattaactat 120 gatatgataa accatgccgc tcaagctgcc tacaattttg aagatggatt ttacatcgat 180 gctgtgaata tggttttcca gttga.atatt agtacatctg acagaccttc ggagaccttt 240 catttgagag atcttgctca acatgatcag atcgaggttg acggctctct cactaggaat 300 gacattttct ttggtgatga tttacacttt gacgcaactg tttttgatcc ggttgcacgc 360 gacctaggtc taaataaaat aagtc:gaaag gataaatttg ttacgattga aaccgctgct 420 aaagccacta agactcgttt ggacttagcg aagcgagtaa acccagaatt caatgcatca 480 gtccaccaac atgaaacaga atatgggact accgcattat atttacttac tgtatgggat 540 gagcaacaaa aagcagctcc taaacagtgg gtcaaggctt tacttggaga agatcgcatt 600 gcttaccgtg agggatataa caaaggcaag acagttaaat ccaataaaca agtgggtgct 660 atgacacaag ccgttcgggc tgtatctttg aatggtagcg caaatggt 708 <210> 26 <211> 236 <212> PRT
<213> Fusarium verticillioides <400> 26 Asp Pro Lys Gly His Glu Phe Arg Ala Ala Gly Pro Tyr Asp Ser Arg Sequence Listing (2) Ser Pro Cys Pro Gly Leu Asn Ala Leu Ala Asn His Gly Tyr Leu Pro Arg Asp Gly Ser Asn Ile Asn Tyr Asp Met Ile Asn His Ala Ala Gln Ala Ala Tyr Asn Phe Glu Asp Gly Phe Tyr Ile Asp Ala Val Asn Met Val Phe Gln Leu Asn Ile Ser Thr Ser Asp Arg Pro Ser Glu Thr Phe His Leu Arg Asp Leu Ala Gln His Asp Gln Ile Glu Val Asp Gly Ser Leu Thr Arg Asn Asp Ile Phe Phe Gly Asp Asp Leu His Phe Asp Ala Thr Val Phe Asp Pro Val Ala Arg Asp Leu Gly Leu Asn Lys Ile Ser Arg Lys Asp Lys Phe Val Thr Ile Glu Thr Ala Ala Lys Ala Thr Lys Thr Arg Leu Asp Leu Ala Lys Arg Val Asn Pro Glu Phe Asn Ala Ser Val His Gln His Glu Thr Glu Tyr Gly Thr Thr Ala Leu Tyr Leu Leu Thr Val Trp Asp Glu Gln Gln Lys Ala Ala Pro Lys Gln Trp Val Lys Ala Leu Leu Gly Glu Asp Arg Ile Ala Tyr Arg Glu Gly Tyr Asn Lys Gly Lys Thr Val Lys Ser Asn Lys Gln Val Gly Ala Met Thr Gln Ala Val Arg Ala Val Ser Leu Asn Gly Ser Ala Asn Gly Sequence Listing (2) <210> 27 <211> 768 <212> DNA
<213> Phytophora infestans <400> 27 atgctttctg tcgttgctgt ctccgcgttg gtcgtctcct ctgtcgccgc tggatcggtc 60 gacataggga agaacggtca agtctccatt gattcgcttt tggtcggcga attctaccga 120 ccttcgagcg acatcgtcac cgggtatgcc gactcgacct cgcccgtccc gttccgtcga 180 tctccgtgtc ctggtctcaa cgcgctcgcc aaccatggtc acatcccgcg cagtggcaag 240 aacatcacac acgaggacct gcgcgcggct cttatgtcgg tgttcaactt tgacagtggc 300 ttgacccagg ttctactgaa ccaattgcct gccaccttct ccctcgacat tatttcgcgc 360 cacaacgtgc ttgagcacga cgcgtccctt gtgcacaacg acgaatactt cggtggtgac 420 ccgatcaata tcaacgagac gatggtggag aatctgctgg gtcgctcgct ggacggcaaa 480 tcgctcggta taactgaggt cggagagacc cgtagggacc gtctggcgga gtgtcgtgcc 540 aacaatccgg agtgcgtctt cggctctaac cagaccacgt tctcgtatct ggaggctgct 600 gtgttcatcg tcggctgcgg tggcaacgtc aacgagtcgg tcactgttga agctgcacac 660 tcgttcgtgt gggatgaacg cattccggac aactatgttg cctcggcggt tcctgtttcg 720 ttcagctaca tgcaatctgt gacggcgaag ctgctaaagt tcgcttaa 768 <210> 28 <211> 255 <212> PRT
<213> Phytophora infestans <400> 28 Met Leu Ser Val Val Ala Val Ser Ala Leu Val Val Ser Ser Val Ala Ala Gly Ser Val Asp Ile Gly Lys Asn Gly Gln Val Ser Ile Asp Ser Leu Leu Val Gly Glu Phe Tyr Arg Pro Ser Ser Asp Ile Val Thr Gly Sequence Listing (2) Tyr Ala Asp Ser Thr Ser Pro Val Pro Phe Arg Arg Ser Pro Cys Pro Gly Leu Asn Ala Leu Ala Asn His Gly His Ile Pro Arg Ser Gly Lys Asn Ile Thr His Glu Asp Leu. Arg Ala Ala Leu Met Ser Val Phe Asn Phe Asp Ser Gly Leu Thr Gln Val Leu Leu Asn Gln Leu Pro Ala Thr Phe Ser Leu Asp Ile Ile Ser Arg His Asn Val Leu Glu His Asp Ala Ser Leu Val His Asn Asp Glu Tyr Phe Gly Gly Asp Pro Ile Asn Ile Asn Glu Thr Met Val Glu Asn Leu Leu Gly Arg Ser Leu Asp Gly Lys Ser Leu Gly Ile Thr Glu Val. Gly Glu Thr Arg Arg Asp Arg Leu Ala Glu Cys Arg Ala Asn Asn Pro Glu Cys Val Phe Gly Ser Asn Gln Thr Thr Phe Ser Tyr Leu Glu Ala Ala Val Phe Ile Val Gly Cys Gly Gly Asn Val Asn Glu Ser Val Thr Val Glu Ala Ala His Ser Phe Val Trp Asp Glu Arg Ile Pro Asp Asri Tyr Val Ala Ser Ala Val Pro Val Ser Phe Ser Tyr Met Gln Ser Val Thr Ala Lys Leu Leu Lys Phe Ala <210> 29 Sequence Listing (2) <211> 765 <212> DNA
<213> Phytophora infestans <400> 29 atggtttcat gcgcctctat cgtcgccgtc gccgtcgctg tcgcctcgtt tgtgagctcg 60 agcagcagcc ggatcctcga ccccaacaag ctcgctgtcg gagagttctt cagaccttcg 120 ggacacgtgg tctcgggctt ctttaacacc acggcatctt tccgccgctc accctgtccc 180 gcacttaacg tgctcgccaa ccacggtcac ctaccgcgta acggccaagg catcaccaag 240 gaccagattg gatctgccct caagtcggtc ttgaaccagg gagacgacgc cgtggttgcg 300 ctactggcga gcttgcccga taccatcgcg cttagcgacc tgtcccagca taacgtctgg 360 gatcacgatg cgtcactcgt tcacactgac gcgcatttcg gccatgaccc gtccgaggtc 420 aacatctcgc tggccaacga cctcttcaac cgtgggaaaa ttagcaataa gctgggcgtc 480 aatgaggtgg gaaatgccta caaggaccgc cgccaagcag gtaaatctta cgagccaaaa 540 tattccctct cggacgcgca aaagacccag tcgtacagtc agggggcagg tctcttgctg 600 gtcttcggcg caaaaaacgg agagagcgtt tcgttggacg tcgctcgctc ctttctcatc 660 gacgagagga tcccgcaggg atggaagccc gctgctactc ctatcactgt tgctgactct 720 cgtgccacct ctgccaagat ccgtgaggtc gccgaggcga tgtag 765 <210> 30 <211> 254 <212> PRT
<213> Phytophora infestans <400> 30 Met Val Ser Cys Ala Ser Ile Val Ala Val Ala Val Ala Val Ala Ser Phe Val Ser Ser Ser Ser Ser Arg Ile Leu Asp Pro Asn Lys Leu Ala Val Gly Glu Phe Phe Arg Pro Ser Gly His Val Val Ser Gly Phe Phe Asn Thr Thr Ala Ser Phe Arg Arg Ser Pro Cys Pro Ala Leu Asn Val Sequence Listing (2) Leu Ala Asn His Gly His Leu Pro Arg Asn Gly Gln Gly Ile Thr Lys Asp Gln Ile Gly Ser Ala Leu Lys Ser Val Leu Asn Gln Gly Asp Asp Ala Val Val Ala Leu Leu Ala Ser Leu Pro Asp Thr Ile Ala Leu Ser Asp Leu Ser Gln His Asn Val Trp Asp His Asp Ala Ser Leu Val His Thr Asp Ala His Phe Gly His Asp Pro Ser Glu Val Asn Ile Ser Leu Ala Asn Asp Leu Phe Asn Arg Gly Lys Ile Ser Asn Lys Leu Gly Val Asn Glu Val Gly Asn Ala Tyr Lys Asp Arg Arg Gln Ala Gly Lys Ser Tyr Glu Pro Lys Tyr Ser Leu Ser Asp Ala Gln Lys Thr Gln Ser Tyr Ser Gln Gly Ala Gly Leu Leu. Leu Val Phe Gly Ala Lys Asn Gly Glu Ser Val Ser Leu Asp Val Ala Arg Ser Phe Leu Ile Asp Glu Arg Ile Pro Gln Gly Trp Lys Pro Ala Ala Thr Pro Ile Thr Val Ala Asp Ser Arg Ala Thr Ser Ala Lys Ile Arg Glu Val Ala Glu Ala Met <210> 31 <211> 855 <212> DNA
<213> Agaricus bisphorous Sequence Listing (2) <400> 31 atgttcatcc ttttcaagat ttttcacgac atcgccttct tttcatggga cctcggtctg 60 acttttgtaa acctcgtcct tccgaacagg ccagtgggga aggttacgcc agagggtcat 120 cccgggtatg gtggaaaatg gcctgaatat attcctccaa aggagggtga tagcagatgc 180 tcatgtccgg cattgaacgc tatggcgaat catggtatac tccctcgtga cggcaaaaac 240 atccagttca aagagatggg cagacttatc aggaccacct acaacttttc ttcgagtttc 300 tgctacttcg taccccacta cgccgccgaa aaagtagtga agaaggatta taacaacgac 360 acgtttaatc ttgacgaact tgatcatcac aatggtatag agcatgacgc ttctctctgt 420 cgcgaagata gcgccctctg tcccgatcaa agcaagcctc atgtcgaata catcacgcaa 480 cttcttgcac acgccacagg caaggacaaa gatggcaatc ctctcctcac catgaaggat 540 cttgcaaagt actcatccaa acgtcgcgct gatgcccgaa gctataataa gaaattcact 600 ctcggcttta ttcatcgcat gtttggaagt tccaactcat ccactatgct gaccattttc 660 ggtggacgtg tcaaagatct tgaaactgtc ttacttgaag aacgacttcc cgagaattgg 720 gaatcgaggg ttcggggaaa gatgggcttg acattcgcgg cattcaatct cacagtgaca 780 ccggtcgagc ttatgatcag tgaaaagaaa tatcttaaag aaaaggctgc caaggaagcc 840 aacaaagacg attaa 855 <210> 32 <211> 284 <212> PRT
<213> Agaricus bisphorous <400> 32 Met Phe Ile Leu Phe Lys Ile. Phe His Asp Ile Ala Phe Phe Ser Trp Asp Leu Gly Leu Thr Phe Val Asn Leu Val Leu Pro Asn Arg Pro Val Gly Lys Val Thr Pro Glu Gly His Pro Gly Tyr Gly Gly Lys Trp Pro Glu Tyr Ile Pro Pro Lys Glu Gly Asp Ser Arg Cys Ser Cys Pro Ala Sequence Listing (2) Leu Asn Ala Met Ala Asn His Gly Ile Leu Pro Arg Asp Gly Lys Asn Ile Gln Phe Lys Glu Met Gly Arg Leu Ile Arg Thr Thr Tyr Asn Phe Ser Ser Ser Phe Cys Tyr Phe Val Pro His Tyr Ala Ala Glu Lys Val Val Lys Lys Asp Tyr Asn Asn Asp Thr Phe Asn Leu Asp Glu Leu Asp His His Asn Gly Ile Glu His Asp Ala Ser Leu Cys Arg Glu Asp Ser Ala Leu Cys Pro Asp Gln Ser Lys Pro His Val Glu Tyr Ile Thr Gln Leu Leu Ala His Ala Thr Gly Lys Asp Lys Asp Gly Asn Pro Leu Leu Thr Met Lys Asp Leu Ala Lys Tyr Ser Ser Lys Arg Arg Ala Asp Ala Arg Ser Tyr Asn Lys Lys Phe Thr Leu Gly Phe Ile His Arg Met Phe Gly Ser Ser Asn Ser Ser Thr Met Leu Thr Ile Phe Gly Gly Arg Val Lys Asp Leu Glu Thr Val Leu Leu Glu Glu Arg Leu Pro Glu Asn Trp Glu Ser Arg Val Arg Gly Lys Met Gly Leu Thr Phe Ala Ala Phe Asn Leu Thr Val Thr Pro Val Glu Leu Met Ile Ser Glu Lys Lys Tyr Leu Lys Glu Lys Ala Ala Lys Glu Ala Asn Lys Asp Asp Sequence Listing (2) <210> 33 <211> 795 <212> DNA
<213> Aspergillus nidulans <400> 33 atgaagacat cagttctatc agcaggcctg agcctcatta cctcggtagt agcgtttccg 60 cactacttgg acattgagcg acgctgggag cccagagaat ggattgcccc aggaccagac 120 gactcccgcg gtccgtgtcc gggtctaaac accctcgcca atcatggcta cctcccccgc 180 aatggtaaag ggataacgct ggatatcctc aaagagggca tgcttgaggg gttcaatatc 240 gagcatggcg atgcggtgct tttgttcttc caggctatca ggaccagtcc aattcctttc 300 tcggacaggt tcgacctcgc cgacctcggc cggcacggca tactggaaca cgatattagt 360 ttgagtcgct ctgatgcata ctttgccgac cccaaaccgt ttaacgagac cgtttgggcc 420 gagacgatgt cgtactttac cacctcaatg atcactgtcg agcagcttgc aaaggctcgg 480 atgggacgcc tggctacctc caagaagacg aaccctgagt tcgagttgtc cttgcttgcc 540 gacgggttca gctggggcga gtgcgcctcg ttcttcgaga tcatggcgga tgggacgact 600 gggacggtgg acaagaaata cattgactac tggttccgaa acgaacgcat gccgaccgag 660 ctcggctggc aacgacgcaa tgtgacaatg aagggctctg agcggatcaa gtacagcctg 720 ttgctcatgg aagctgccgg cgtgaagaag cgagacatga catcggatgc gtatggtctg 780 cctatcgtcc actga 795 <210> 34 <211> 264 <212> PRT
<213> Aspergillus nidulans <400> 34 Met Lys Thr Ser Val Leu Ser Ala Gly Leu Ser Leu Ile Thr Ser Val Val Ala Phe Pro His Tyr Leu Asp Ile Glu Arg Arg Trp Glu Pro Arg Glu Trp Ile Ala Pro Gly Pro Asp Asp Ser Arg Gly Pro Cys Pro Gly Sequence Listing (2) Leu Asn Thr Leu Ala Asn His Gly Tyr Leu Pro Arg Asn Gly Lys Gly Ile Thr Leu Asp Ile Leu Lys Glu Gly Met Leu Glu Gly Phe Asn Ile Glu His Gly Asp Ala Val Leu Leu Phe Phe Gln Ala Ile Arg Thr Ser Pro Ile Pro Phe Ser Asp Arg Phe Asp Leu Ala Asp Leu Gly Arg His Gly Ile Leu Glu His Asp Ile Ser Leu Ser Arg Ser Asp Ala Tyr Phe Ala Asp Pro Lys Pro Phe Asn Glu Thr Val Trp Ala Glu Thr Met Ser Tyr Phe Thr Thr Ser Met Ile Thr Val Glu Gln Leu Ala Lys Ala Arg Met Gly Arg Leu Ala Thr Ser Lys Lys Thr Asn Pro Glu Phe Glu Leu Ser Leu Leu Ala Asp Gly Phe Ser Trp Gly Glu Cys Ala Ser Phe Phe Glu Ile Met Ala Asp Gly Thr Thr Gly Thr Val Asp Lys Lys Tyr Ile Asp Tyr Trp Phe Arg Asn Glu Arg Met Pro Thr Glu Leu Gly Trp Gln Arg Arg Asn Val Thr Met Lys Gly Ser Glu Arg Ile Lys Tyr Ser Leu Leu Leu Met Glu Ala Ala Gly Val Lys Lys Arg Asp Met Thr Ser Asp Sequence Listing (2) Ala Tyr Gly Leu Pro Ile Val His <210> 35 <211> 918 <212> DNA
<213> Aspergillus nidulans <400> 35 atgttgctca agagcatcca gaacattgtc tgtggcctcg tgccaacgtt cttcctgttt 60 ggctctgcag ctgccgagct ggactttgaa cagtggcatc cagcgggact gggagacttg 120 cgctgcggct gcccggccat gaacagtctt gccaaccacg ggttcatcaa ccataacggc 180 agcaacatca cggtcaacga ggtcatcccg ttgatgcaag aggtctttca tctcagtgaa 240 gagctcgcca cgattgtcac gggcctcgcc gtcctctcgg cggacgaccc tgcctcgggt 300 attttcaacc ttgatatgct gaaccgccac aacattttcg agcacgacgc ctcactgacc 360 cgcaaggatt tctacctggg cggcgacgga catactattg accagcccac gctggacgag 420 ttcctcagct acttcgacgg caaagaatgg atcgacctca acgacgccgc agcagcccgc 480 tatgcgcgcg tcctcgactc ccgcgagaag aacccgagct tcctctacca ggaccagcag 540 ctcattactt cgtacggcga gacgatcaag tacttccgaa ccatggtcga cccgcgctca 600 aacaagacat ccgccgagtt cgtcaggatc ttgttcaccg aggagcggct gcccgtcagg 660 gaagggtggc agcgcccgcg agaagagatc agtgggttct cgctggccag cgatgtcgtt 720 cagctggcgc tgcgcacccc agagaagttc attggcatgc cgttcgacca gcgtccgttt 780 gcagagcagg cctttgaccc gctgccatgg cagcggcctc ccatctggac tcccccgaac 840 tacccgggtt tcagtaagag gcatttctct gagcttgtcg ggaggtttgc gaagaaggcc 900 cttccgtttc gtgcttga 918 <210> 36 <211> 305 <212> PRT
<213> Aspergillus nidulans <400> 36 Met Leu Leu Lys Ser Ile Gln Asn Ile Val Cys Gly Leu Val Pro Thr Sequence Listing (2) Phe Phe Leu Phe Gly Ser Ala Ala Ala Glu Leu Asp Phe Glu Gln Trp His Pro Ala Gly Leu Gly Asp Leu Arg Cys Gly Cys Pro Ala Met Asn Ser Leu Ala Asn His Gly Phe Ile Asn His Asn Gly Ser Asn Ile Thr Val Asn Glu Val Ile Pro Leu Met Gln Glu Val Phe His Leu Ser Glu Glu Leu Ala Thr Ile Val Thr Gly Leu Ala Val Leu Ser Ala Asp Asp Pro Ala Ser Gly Ile Phe Asn Leu Asp Met Leu Asn Arg His Asn Ile Phe Glu His Asp Ala Ser Leu Thr Arg Lys Asp Phe Tyr Leu Gly Gly Asp Gly His Thr Ile Asp Gln Pro Thr Leu Asp Glu Phe Leu Ser Tyr Phe Asp Gly Lys Glu Trp Ile Asp Leu Asn Asp Ala Ala Ala Ala Arg Tyr Ala Arg Val Leu Asp Ser Arg Glu Lys Asn Pro Ser Phe Leu Tyr Gln Asp Gln Gln Leu Ile Thr Ser Tyr Gly Glu Thr Ile Lys Tyr Phe Arg Thr Met Val Asp Pro Arg Ser Asn Lys Thr Ser Ala Glu Phe Val Arg Ile Leu Phe Thr Glu Glu Arg Leu Pro Val Arg Glu Gly Trp Gln Arg Pro Arg Glu Glu Ile Ser Gly Phe Ser Leu Ala Ser Asp Val Val Sequence Listing (2) Gln Leu Ala Leu Arg Thr Pro Glu Lys Phe Ile Gly Met Pro Phe Asp Gln Arg Pro Phe Ala Glu Gln Ala Phe Asp Pro Leu Pro Trp Gln Arg Pro Pro Ile Trp Thr Pro Pro Asn Tyr Pro Gly Phe Ser Lys Arg His Phe Ser Glu Leu Val Gly Arg Phe Ala Lys Lys Ala Leu Pro Phe Arg Ala <210> 37 <211> 816 <212> DNA
<213> Aspergillus nidulans <400> 37 atgaagctca ctgcaccgtc gctccttgcc ctagccggcg tcgtctccgc caccctccat 60 ctagacctga acctgggcgg atcgcaccca cacgttcatg cttccaactc caaactcggt 120 gccgagaacc cgcatgcttg gattgctgct ggggatggag acgggtatct tcgtgcagcc 180 cgcagtccct gcccgatgat gaacaccctt gccaaccacg gctatatcca ccgagacggg 240 cgtaacatca cacgcgaggg cctggccaat ggactggttg actcaatcaa cttctcccaa 300 gaattggccg agagcatgtt tgaca.atgtc ctggccatgg acccaaccgc cacaaacttt 360 gatctagact ccctgaatgc gcacaacgtc ggcgagcatg atgcctctct cagccgcgcg 420 gatgcctact tcggcaacaa ccacgtcttt aatcccaacg tattcgccca aacacgcgcc 480 tactggacgc accctacagt gaccgccgcc atgcttgcaa atagcaagat cgcacggcag 540 ctgcacagca aagcattcaa ccctgcctac acgtacacag agaaaaccga gcagacgagc 600 tacacggaga ccggtttccc gatcctggcc tttggcgata tcgaggccgg cacggtgaac 660 cgtgacttcg tggagtactg gtttgaaaat gagcggttgc catcagaggt cggatggact 720 gtgcgtgagg agccggtgac gccgqagcag ctggcagccg tgacgcagat gatcgcggag 780 Sequence Listing (2) gccgtcaagt tggtgacggg ggagaagaag cagtaa 816 <210> 38 <211> 271 <212> PRT
<213> Aspergillus nidulans <400> 38 Met Lys Leu Thr Ala Pro Ser Leu Leu Ala Leu Ala Gly Val Val Ser Ala Thr Leu His Leu Asp Leu Asn Leu Gly Gly Ser His Pro His Val His Ala Ser Asn Ser Lys Leu Gly Ala Glu Asn Pro His Ala Trp Ile Ala Ala Gly Asp Gly Asp Gly Tyr Leu Arg Ala Ala Arg Ser Pro Cys Pro Met Met Asn Thr Leu Ala Asn His Gly Tyr Ile His Arg Asp Gly Arg Asn Ile Thr Arg Glu Gly Leu Ala Asn Gly Leu Val Asp Ser Ile Asn Phe Ser Gln Glu Leu Ala Glu Ser Met Phe Asp Asn Val Leu Ala Met Asp Pro Thr Ala Thr Asn Phe Asp Leu Asp Ser Leu Asn Ala His Asn Val Gly Glu His Asp Ala Ser Leu Ser Arg Ala Asp Ala Tyr Phe Gly Asn Asn His Val Phe Asn. Pro Asn Val Phe Ala Gln Thr Arg Ala Tyr Trp Thr His Pro Thr Val Thr Ala Ala Met Leu Ala Asn Ser Lys Sequence Listing (2) Ile Ala Arg Gln Leu His Ser Lys Ala Phe Asn Pro Ala Tyr Thr Tyr Thr Glu Lys Thr Glu Gln Thr Ser Tyr Thr Glu Thr Gly Phe Pro Ile Leu Ala Phe Gly Asp Ile Glu Ala Gly Thr Val Asn Arg Asp Phe Val Glu Tyr Trp Phe Glu Asn Glu Arg Leu Pro Ser Glu Val Gly Trp Thr Val Arg Glu Glu Pro Val Thr Pro Glu Gln Leu Ala Ala Val Thr Gln Met Ile Ala Glu Ala Val Lys Leu Val Thr Gly Glu Lys Lys Gln <210> 39 <211> 807 <212> DNA
<213> Aspergillus nidulans <400> 39 atgaaggttg caactccatt gatggcgatc ctcggcctat ctgcggccaa ccctcactac 60 ggggcgggtg atatctccca atggaccggc cccggtgcag gcgattaccg aggcccctgc 120 cccatgatga acacacttgc caaccataat tttctgccgc atgacggacg caatatcacg 180 cgggataaac ttatccgtgc cctaggggaa gccctgaact ttcatccaag cctggcctcg 240 ctcatgttcg acatggcaat tgttgtgaac cctgaaccta atgcgacctg gtttaccctc 300 gaccagctaa accagcacaa cattcttgag cacgacgcta gcctcagccg cacagacgcc 360 ttcttcggta acaaccacat cttcaacgcc agcatatttg cggaaacgac gcactactgg 420 acgaacacca ccctgactcc gctcatgctc gctaacagca agctcgcacg gcagatcacc 480 tcgcgcgcat tcaacccgac gtacaccttc acgaacagca cagagcaatt cagcctaggc 540 gaagttgcgg cgccagtcgt ggcgt.ttggg aacctgaatg acggaacggt ggaaaaagag 600 ctggtggagt acttcttccg aaacgagcgg tttccgacgc acttgggctg gaagacacgc 660 gatcaggtag tgcagcttgc ggatatcaca aagctgtcca ggattattgg aggcatgacg 720 Sequence Listing (2) gacttgctca ccagtaccgg tgatgctgca actgccagcc ccgctagagt gaagagagct 780 gatctgcacg ctggcttcag aggctga 807 <210> 40 <211> 268 <212> PRT
<213> Aspergillus nidulans <400> 40 Met Lys Val Ala Thr Pro Leu Met Ala Ile Leu Gly Leu Ser Ala Ala Asn Pro His Tyr Gly Ala Gly Asp Ile Ser Gln Trp Thr Gly Pro Gly Ala Gly Asp Tyr Arg Gly Pro Cys Pro Met Met Asn Thr Leu Ala Asn His Asn Phe Leu Pro His Asp Gly Arg Asn Ile Thr Arg Asp Lys Leu Ile Arg Ala Leu Gly Glu Ala Leu Asn Phe His Pro Ser Leu Ala Ser Leu Met Phe Asp Met Ala Ile Val Val Asn Pro Glu Pro Asn Ala Thr Trp Phe Thr Leu Asp Gln Leu. Asn Gln His Asn Ile Leu Glu His Asp Ala Ser Leu Ser Arg Thr Asp Ala Phe Phe Gly Asn Asn His Ile Phe Asn Ala Ser Ile Phe Ala Glu Thr Thr His Tyr Trp Thr Asn Thr Thr Leu Thr Pro Leu Met Leu Ala Asn Ser Lys Leu Ala Arg Gln Ile Thr Ser Arg Ala Phe Asn Pro Thr Tyr Thr Phe Thr Asn Ser Thr Glu Gln Sequence Listing (2) Phe Ser Leu Gly Glu Val Ala Ala Pro Val Val Ala Phe Gly Asn Leu Asn Asp Gly Thr Val Glu Lys Glu Leu Val Glu Tyr Phe Phe Arg Asn Glu Arg Phe Pro Thr His Leu Gly Trp Lys Thr Arg Asp Gln Val Val Gln Leu Ala Asp Ile Thr Lys Leu Ser Arg Ile Ile Gly Gly Met Thr Asp Leu Leu Thr Ser Thr Gly Asp Ala Ala Thr Ala Ser Pro Ala Arg Val Lys Arg Ala Asp Leu His Ala Gly Phe Arg Gly <210> 41 <211> 801 <212> DNA
<213> Gibberella zeae <400> 41 atgaagttct ctaccttgat tgctgcctct ggtaccgttg cgaccacctt cgctctccct 60 cagtttctac ccacggctgg tgccgagtgg actcctcaag aatgggttgc tcctggcgca 120 aatgactctc gtggtccttg ccctggtctt aacacattgg ccaaccatgg ctaccttccc 180 catgacggaa agaacatcga tctgaagaaa cttgccgatg gaatgcttgc tggtttcaac 240 atcgagaagg gtgatgctct ccttctcttc acccaggcca tcaggacaag ccctgactat 300 ccttcaaccc gtactttcga tctagcggat cttggtcgtc acaatatcct cgagcacgat 360 atttccatca gtcgctcgga cgctttcttt gctgacccaa accccttcaa caaaactgtt 420 tgggccgaga ccctaacata cttccccgac gacatgatca ccgtcgagca agttgcgaaa 480 gcacgaatgg gtcgtcttgc aacctcgaag aagaccaacc cccagcactc gctgtctaag 540 ttggctgacg gcttcagctg gggcgagatg gcttctttct ttgagatcat ggctgatggt 600 actaccggca cggtagagaa gaagtacatc gagtactggt ttaagaatga gcgtctgcct 660 Sequence Listing (2) acagaaattg gatggcagcg acgaaccaca accatgcgcg gtattgagcg catcgagttc 720 actagaaggc tcatggaggc tgctggcgtt tcccgccgtg atatcaccag tgatgcttat 780 ggtcgcccgc tggaacagta a 801 <210> 42 <211> 266 <212> PRT
<213> Gibberella zeae <400> 42 Met Lys Phe Ser Thr Leu Ile Ala Ala Ser Gly Thr Val Ala Thr Thr Phe Ala Leu Pro Gln Phe Leu Pro Thr Ala Gly Ala Glu Trp Thr Pro Gln Glu Trp Val Ala Pro Gly Ala Asn Asp Ser Arg Gly Pro Cys Pro Gly Leu Asn Thr Leu Ala Asn His Gly Tyr Leu Pro His Asp Gly Lys Asn Ile Asp Leu Lys Lys Leu Ala Asp Gly Met Leu Ala Gly Phe Asn Ile Glu Lys Gly Asp Ala Leu Leu Leu Phe Thr Gln Ala Ile Arg Thr Ser Pro Asp Tyr Pro Ser Thr Arg Thr Phe Asp Leu Ala Asp Leu Gly Arg His Asn Ile Leu Glu His Asp Ile Ser Ile Ser Arg Ser Asp Ala Phe Phe Ala Asp Pro Asn Pro Phe Asn Lys Thr Val Trp Ala Glu Thr Leu Thr Tyr Phe Pro Asp Asp Met Ile Thr Val Glu Gln Val Ala Lys Sequence Listing (2) Ala Arg Met Gly Arg Leu Ala Thr Ser Lys Lys Thr Asn Pro Gln His Ser Leu Ser Lys Leu Ala Asp Gly Phe Ser Trp Gly Glu Met Ala Ser Phe Phe Glu Ile Met Ala Asp Gly Thr Thr Gly Thr Val Glu Lys Lys Tyr Ile Glu Tyr Trp Phe Lys Asn Glu Arg Leu Pro Thr Glu Ile Gly Trp Gln Arg Arg Thr Thr Thr Met Arg Gly Ile Glu Arg Ile Glu Phe Thr Arg Arg Leu Met Glu Ala Ala Gly Val Ser Arg Arg Asp Ile Thr Ser Asp Ala Tyr Gly Arg Prc Leu Glu Gln <210> 43 <211> 774 <212> DNA
<213> Magnaporthe grisea <400> 43 atgtacgcca agcagctcat cgtggccgcg ctgacggcaa cagccacggc cttccccatg 60 atggccagct caggcgcggg cgaatccatc gacgccacga tcgtggcccg gcaggccccc 120 gcagaccacc agttccaggc ccccggcgcc aacgacgtgc gctcgccctg ccccatgctc 180 aacaccctgg ccaaccacgg ctacctgccc cgcaacgggc tcaacatcga catgcccacg 240 ctcaagcagg cctttaccga cggcgtgaac ctggcgcccg acgcgaccga gttcgccggc 300 aagaaggcgc tcgtggccgg cgacggcacg cgcttcaacc tcgaccagct caagaagcac 360 cacatcctgg agcacgacgg ctccctgtcc aggcaggaca tcgccctggg caacaacctg 420 gtcctggacc agaccatctg gtccaagacc ctgcaggact tccccaacga ccagatcagc 480 ctcaccgact cggcccgcgc caggaagaac aggcacgccg ccgcaaaggc cgccaacaag 540 gactacgacc tgccccttga ggagcagatg ttcaccttta tcgagtcggc cctgtaccag 600 Sequence Listing (2) agcgtgtttg gcaagggcac cgacggcggt gcgcgcaagg actgggtgag gacgttgttt 660 gagcaggaac gtcttccttt cgagcagggc tggtcgaggg ccacttctgc catcagcact 720 ccgacggtcc ttattctcac tacaaagatc gctgccctga ccgtggtcgt ataa 774 <210> 44 <211> 257 <212> PRT
<213> Magnaporthe grisea <400> 44 Met Tyr Ala Lys Gln Leu Ile Val Ala Ala Leu Thr Ala Thr Ala Thr Ala Phe Pro Met Met Ala Ser Ser Gly Ala Gly Glu Ser Ile Asp Ala Thr Ile Val Ala Arg Gln Ala Pro Ala Asp His Gln Phe Gln Ala Pro Gly Ala Asn Asp Val Arg Ser Pro Cys Pro Met Leu Asn Thr Leu Ala Asn His Gly Tyr Leu Pro Arg Asn Gly Leu Asn Ile Asp Met Pro Thr Leu Lys Gln Ala Phe Thr Asp Gly Val Asn Leu Ala Pro Asp Ala Thr Glu Phe Ala Gly Lys Lys Ala. Leu Val Ala Gly Asp Gly Thr Arg Phe Asn Leu Asp Gln Leu Lys Lys His His Ile Leu Glu His Asp Gly Ser Leu Ser Arg Gln Asp Ile Ala Leu Gly Asn Asn Leu Val Leu Asp Gln Thr Ile Trp Ser Lys Thr Leu Gln Asp Phe Pro Asn Asp Gln Ile Ser Sequence Listing (2) Leu Thr Asp Ser Ala Arg Ala Arg Lys Asn Arg His Ala Ala Ala Lys Ala Ala Asn Lys Asp Tyr Asp Leu Pro Leu Glu Glu Gln Met Phe Thr Phe Ile Glu Ser Ala Leu Tyr Gln Ser Val Phe Gly Lys Gly Thr Asp Gly Gly Ala Arg Lys Asp Trp Val Arg Thr Leu Phe Glu Gln Glu Arg Leu Pro Phe Glu Gln Gly Trp Ser Arg Ala Thr Ser Ala Ile Ser Thr Pro Thr Val Leu Ile Leu Thr Thr Lys Ile Ala Ala Leu Thr Val Val Val <210> 45 <211> 729 <212> DNA
<213> Magnaporthe grisea <400> 45 atggtctcca cacgcacctt gacggcactc ctatgcaccg tcgtcccgct cacgtgggcc 60 tacccgagca tgatcgaagc acgagatgat catcccttca tggcccccag ccccaacgac 120 cgcagaagcc cctgccccat gatgaacacg ctcgccaacc acggcttcat cccgcggacc 180 aacatctcga tggaccaact ggtgtcggca ctggagctga gcatcggcct cgagggcgcc 240 gcgaccagga tcgtgggcgg caaggccctt gccacctcga cgacgggcaa cccaaacacc 300 ttcaacctcg acgacctcgt caagcacggc atcctcgagc acgacggctc gctgtcacgc 360 tccgacctgg ccgatggcga caaccactcc ttcaacaaga ccctctggga cacgacgctc 420 acctttttca aggacgacaa gatctccatc gcccagggcg ccgccgccag gaacgccgcc 480 atcgctgccg ccaaggccag gaacccaaag ttcgccatgg ccgctggcga cgcgcagaac 540 agcgccgtcg agtcggcgct ctacctgcgc gtatttggtg gtgtcaatgg cgatgcccgg 600 Sequence Listing (2) aaggactggg tcaagattct ttttgagcaa gagcgcatgc cctacgccga aggtttccca 660 cagaagcccg caggcaagat cacagcccag gaaatacttg gtattgtgtc aaagctcact 720 gccgcttag 729 <210> 46 <211> 242 <212> PRT
<213> Magnaporthe grisea <400> 46 Met Val Ser Thr Arg Thr Leu Thr Ala Leu Leu Cys Thr Val Val Pro Leu Thr Trp Ala Tyr Pro Ser Met Ile Glu Ala Arg Asp Asp His Pro Phe Met Ala Pro Ser Pro Asn Asp Arg Arg Ser Pro Cys Pro Met Met Asn Thr Leu Ala Asn His Gly Phe Ile Pro Arg Thr Asn Ile Ser Met Asp Gln Leu Val Ser Ala Leu. Glu Leu Ser Ile Gly Leu Glu Gly Ala Ala Thr Arg Ile Val Gly Gly Lys Ala Leu Ala Thr Ser Thr Thr Gly Asn Pro Asn Thr Phe Asn Leu Asp Asp Leu Val Lys His Gly Ile Leu Glu His Asp Gly Ser Leu Ser Arg Ser Asp Leu Ala Asp Gly Asp Asn His Ser Phe Asn Lys Thr Leu Trp Asp Thr Thr Leu Thr Phe Phe Lys Asp Asp Lys Ile Ser Ile Ala Gln Gly Ala Ala Ala Arg Asn Ala Ala Sequence Listing (2) Ile Ala Ala Ala Lys Ala Arg Asn Pro Lys Phe Ala Met Ala Ala Gly Asp Ala Gln Asn Ser Ala Val Glu Ser Ala Leu Tyr Leu Arg Val Phe Gly Gly Val Asn Gly Asp Ala Arg Lys Asp Trp Val Lys Ile Leu Phe Glu Gln Glu Arg Met Pro Tyr Ala Glu Gly Phe Pro Gln Lys Pro Ala Gly Lys Ile Thr Ala Gln Glu Ile Leu Gly Ile Val Ser Lys Leu Thr Ala Ala <210> 47 <211> 726 <212> DNA
<213> Magnaporthe grisea <400> 47 atgctcgtcc gaacctttgt cgctgttctg ctgcctgtgc aagcacttgg attggtagtc 60 cctagagacg accaccagtg gcaagcaccc gggccaaacg atcgccgcag cccctgcccc 120 tttctcaaca cggcatccaa ccacggcttc ctcccccgcg acggcttcaa cgtttcgcac 180 cagcaaatct ttgacggctt caaggaagcc ctcaactttg actcgtcggc gctgctcgcg 240 gccgtcaccc tggggcagac gacgtcgacg acggggcacc ccagcaccat gcacatggac 300 gacctcaacg tgcacaacgt ctttgagcac gacgccagcc tgagccgcaa cgatacatac 360 tttggccccg ccatcaagtt cgaaaagtcc atctgggccc agaccctcac gcacttcccg 420 gacccctaca tctcgctgtc gcaggccaag gccgccctgg aggcccgcat cgccgagtcc 480 aaggccacca acccgcagca cgtctttggc gtgctggcgc agtccatcgt ggacatcgag 540 acggcgacct atacgtttgg caccggcggg ggtgtcaatg gcggcgcgaa ccgggagtgg 600 ttgaaggtta tttttcagga ggagcggttc ccatacgagc ttggtttcca caagtttgag 660 caggtggcca cgggagaaat ggtggaccag tttatcgcaa acctcaaggc tgcgccgccg 720 Sequence Listing (2) gtgtaa 726 <210> 48 <211> 241 <212> PRT
<213> Magnaporthe grisea <400> 48 Met Leu Val Arg Thr Phe Val Ala Val Leu Leu Pro Val Gln Ala Leu Gly Leu Val Val Pro Arg Asp Asp His Gln Trp Gln Ala Pro Gly Pro Asn Asp Arg Arg Ser Pro Cys Pro Phe Leu Asn Thr Ala Ser Asn His Gly Phe Leu Pro Arg Asp Gly Phe Asn Val Ser His Gln Gln Ile Phe Asp Gly Phe Lys Glu Ala Leu Asn Phe Asp Ser Ser Ala Leu Leu Ala Ala Val Thr Leu Gly Gln Thr Thr Ser Thr Thr Gly His Pro Ser Thr Met His Met Asp Asp Leu Asn Val His Asn Val Phe Glu His Asp Ala Ser Leu Ser Arg Asn Asp Thr Tyr Phe Gly Pro Ala Ile Lys Phe Glu Lys Ser Ile Trp Ala Gln Thr Leu Thr His Phe Pro Asp Pro Tyr Ile Ser Leu Ser Gln Ala Lys Ala Ala Leu Glu Ala Arg Ile Ala Glu Ser Lys Ala Thr Asn Pro Gln His Val Phe Gly Val Leu Ala Gln Ser Ile Sequence Listing (2) Val Asp Ile Glu Thr Ala Thr Tyr Thr Phe Gly Thr Gly Gly Gly Val Asn Gly Gly Ala Asn Arg Glu Trp Leu Lys Val Ile Phe Gln Glu Glu Arg Phe Pro Tyr Glu Leu Gly Phe His Lys Phe Glu Gln Val Ala Thr Gly Glu Met Val Asp Gln Phe Ile Ala Asn Leu Lys Ala Ala Pro Pro Val <210> 49 <211> 819 <212> DNA
<213> Neurosporra crassa <400> 49 atgtcctcct ctaccaatat aacctccaca gccctcggca tcaaagtcat gtccttcctc 60 acctccatct tggcagtcgg catcgaagcc tacgccaaac ttacggggaa aaacgaagat 120 acagaggacc attcatggca gaagcctgga cctgacgacc gccgctcccc ctgccccatg 180 ctcaacgccc tcgccaacca cggctacctc ccgcactcgg gccgcgacat ctccctcgcc 240 caactcatgc acggtctaca gaggggaatc aacctctccc ccaacgctac tttaatcgtc 300 ggcatcaaag ccctccaagc cagcaccacg gggcactgga ccaccttcca cctatccgac 360 ctcaacacgc acggcgtcat cgaac:acgac gggtccttgt cccgcgcgga cttgtcggcc 420 ccgccgaatt acgataacca tacgt.ttgtc ccggagattt gggcggctac gttgcaggtt 480 ttgggtgaga atgaagaaag aattagtatc gagaaggcgg cgaggatgcg cttggccaga 540 attgatgaag aagagggacc gaagagaggg aatccgaatt tcagcatgag tagtgaagac 600 aagaggttta gtgcgattga gacggcgttg tatttgaggg tgtttggaag ggggaccgag 660 ggcggggcgg agacgaggtg ggtgagggtt ttgtttgagg aagaacgact cccgattgaa 720 gagggattca agagatccga caaaccccta acgattgccg aggttttgga gttgcagaga 780 aaggtcgagg ctgtcggtgc tactcctgat ccgaagtaa 819 Sequence Listing (2) <210> 50 <211> 272 <212> PRT
<213> Neurosporra crassa <400> 50 Met Ser Ser Ser Thr Asn Ile Thr Ser Thr Ala Leu Gly Ile Lys Val Met Ser Phe Leu Thr Ser Ile Leu Ala Val Gly Ile Glu Ala Tyr Ala Lys Leu Thr Gly Lys Asn Glu Asp Thr Glu Asp His Ser Trp Gln Lys Pro Gly Pro Asp Asp Arg Arg Ser Pro Cys Pro Met Leu Asn Ala Leu Ala Asn His Gly Tyr Leu Pro His Ser Gly Arg Asp Ile Ser Leu Ala Gln Leu Met His Gly Leu Gln Arg Gly Ile Asn Leu Ser Pro Asn Ala Thr Leu Ile Val Gly Ile Lys Ala Leu Gln Ala Ser Thr Thr Gly His Trp Thr Thr Phe His Leu Ser Asp Leu Asn Thr His Gly Val Ile Glu His Asp Gly Ser Leu Ser Arg Ala Asp Leu Ser Ala Pro Pro Asn Tyr Asp Asn His Thr Phe Val Pro Glu Ile Trp Ala Ala Thr Leu Gln Val Leu Gly Glu Asn Glu Glu Arg Ile Ser Ile Glu Lys Ala Ala Arg Met Arg Leu Ala Arg Ile Asp Glu Glu Glu Gly Pro Lys Arg Gly Asn Pro Sequence Listing (2) Asn Phe Ser Met Ser Ser Glu Asp Lys Arg Phe Ser Ala Ile Glu Thr Ala Leu Tyr Leu Arg Val Phe Gly Arg Gly Thr Glu Gly Gly Ala Glu Thr Arg Trp Val Arg Val Leu. Phe Glu Glu Glu Arg Leu Pro Ile Glu Glu Gly Phe Lys Arg Ser Asp Lys Pro Leu Thr Ile Ala Glu Val Leu Glu Leu Gln Arg Lys Val Glu Ala Val Gly Ala Thr Pro Asp Pro Lys <210> 51 <211> 936 <212> DNA
<213> Emericella <400> 51 atgttgctca agagcatcca gaacattgtc tgtggcctcg tgccaacgtt cttcctgttt 60 ggctctgcag ctgccgagct ggactttgaa cagtggcatc cagcgggact gggagacttg 120 cgctgcggct gcccggccat gaacagtctt gccaaccacg ggttcatcaa ccataacggc 180 agcaacatca cggtcaacga ggtcatcccg ttgatgcaag aggtctttca tctcagtgaa 240 gagctcgcca cgattgtcac gggcctcgcc gtcctctcgg cggacgaccc tgcctcgggt 300 attttcaacc ttgatatgct gaaccgccac aacattttcg agcacgacgc ctcactgacc 360 cgaaaggatt tctacctggg cggcgacgga catactattg accagcccac gctggacgag 420 ttcctcagct acttcgacgg caaagaatgg atcgacctca acgacgccgc agcagcccgc 480 tatgcgagag tcctcgactc ccgcgagaag aacccgagct tcctctacca ggaccagcag 540 ctcattactt cgtacggcga gacgatcaag tacttccgaa ccatggtcga cccgcgctca 600 aacaagacat ccgccgagtt cgtcaggatc ttgttcaccg aggagcggct gcccgtcagg 660 aagggtggca gcgcccgcga gaagagatca gtgggttctc gctggccagc gatgtcgttc 720 agctggcgct gcgcacccca gagaagttca ttggcatgcc gttcgaccag cgtccgtttg 780 Sequence Listing (2) cagagcaggc ctttgacccg ctgccatggc agcggcctcc catctggact cccccgaact 840 acccgggttt cagtaagagg catttctctg agcttgtcgg gaggtttgcg aagaaggccc 900 ttccgtttcg tgcttgatag acatctatct ttctga 936 <210> 52 <211> 311 <212> PRT
<213> Emericella <400> 52 Met Leu Leu Lys Ser Ile Gln Asn Ile Val Cys Gly Leu Val Pro Thr Phe Phe Leu Phe Gly Ser Ala Ala Ala Glu Leu Asp Phe Glu Gln Trp His Pro Ala Gly Leu Gly Asp Leu Arg Cys Gly Cys Pro Ala Met Asn Ser Leu Ala Asn His Gly Phe Ile Asn His Asn Gly Ser Asn Ile Thr Val Asn Glu Val Ile Pro Leu Met Gln Glu Val Phe His Leu Ser Glu Glu Leu Ala Thr Ile Val Thr Gly Leu Ala Val Leu Ser Ala Asp Asp Pro Ala Ser Gly Ile Phe Asn Leu Asp Met Leu Asn Arg His Asn Ile Phe Glu His Asp Ala Ser Leu Thr Arg Lys Asp Phe Tyr Leu Gly Gly Asp Gly His Thr Ile Asp Gln Pro Thr Leu Asp Glu Phe Leu Ser Tyr Phe Asp Gly Lys Glu Trp Ile Asp Leu Asn Asp Ala Ala Ala Ala Arg Sequence Listing (2) Tyr Ala Arg Val Leu Asp Ser Arg Glu Lys Asn Pro Ser Phe Leu Tyr Gln Asp Gln Gln Leu Ile Thr Ser Tyr Gly Glu Thr Ile Lys Tyr Phe Arg Thr Met Val Asp Pro Arg Ser Asn Lys Thr Ser Ala Glu Phe Val Arg Ile Leu Phe Thr Glu Glu Arg Leu Pro Val Arg Lys Gly Gly Ser Ala Arg Glu Lys Arg Ser Val Gly Ser Arg Trp Pro Ala Met Ser Phe Ser Trp Arg Cys Ala Pro Gln Arg Ser Ser Leu Ala Cys Arg Ser Thr Ser Val Arg Leu Gln Ser Arg Pro Leu Thr Arg Cys His Gly Ser Gly Leu Pro Ser Gly Leu Pro Arg Thr Thr Arg Val Ser Val Arg Gly Ile Ser Leu Ser Leu Ser Gly Gly Leu Arg Arg Arg Pro Phe Arg Phe Val Leu Asp Arg His Leu Ser Phe <210> 53 <211> 837 <212> DNA
<213> Bacteria <400> 53 atgccgtaca tcaccgtggg ccaggagaac accaacccca tcgagctcta cttcgaggac 60 caaggagacg ggcagcccgt cgtcctcatc cacggcttcc cgctcgacgg ccactcctgg 120 gagcgccaga gcgccgcgct gctcgacgcc ggccaccggg tgatcacgta cgaccgccgc 180 ggcttcgggc agtcctccca gccgaccacc ggctacgact acgacacctt cgccgccgac 240 Sequence Listing (2) ctgaacaccg tgatggagac cctcgatctg aaggacgccg tcctcgtcgg cttctccatg 300 ggcaccggcg aagtcgcccg ttacgtcgcc acgtacggct ccggccgcgt cgccaaggtc 360 gccttcctcg cctccctgga gccctgcctg ctcaagagcg acgacaaccc ggacggcgtc 420 gccccgaagg agttcttcga cggcgtcgtc gccgcagtga aggccgaccg gtacgcctac 480 tacacggcct tcttcaacga cttctacaac ctcgacgaga acctcggcag ccgcatcagc 540 gaggaggccg tccgcaacag ctggaacacc gcggcccgcg gcggctcctt cgccgcctcc 600 gccgcgccgg cgacctggta caccgatttc cgtgccgaca tccccgccgt cgacgtgccg 660 gcgctgatcc tgcacggcac cgccgaccgc atcctgcccg ccgagggcac cgcgcgcccc 720 ttccacaagg cgctcccgtc ggccgactac gtcgagatcg agggcgcccc gcacggtctg 780 ctgtggaccc acgccgagga ggtcaacacc gccctcctcg ccttcctggc gaagtga 837 <210> 54 <211> 278 <212> PRT
<213> Bacteria <400> 54 Met Pro Tyr Ile Thr Val Gly Gln Glu Asn Thr Asn Pro Ile Glu Leu Tyr Phe Glu Asp Gln Gly Asp Gly Gln Pro Val Val Leu Ile His Gly Phe Pro Leu Asp Gly His Ser Trp Glu Arg Gln Ser Ala Ala Leu Leu Asp Ala Gly His Arg Val Ile Thr Tyr Asp Arg Arg Gly Phe Gly Gln Ser Ser Gln Pro Thr Thr Gly Tyr Asp Tyr Asp Thr Phe Ala Ala Asp Leu Asn Thr Val Met Glu Thr Leu Asp Leu Lys Asp Ala Val Leu Val Gly Phe Ser Met Gly Thr Gly Glu Val Ala Arg Tyr Val Ala Thr Tyr Sequence Listing (2) Gly Ser Gly Arg Val Ala Lys Val Ala Phe Leu Ala Ser Leu Glu Pro Cys Leu Leu Lys Ser Asp Asp Asn Pro Asp Gly Val Ala Pro Lys Glu Phe Phe Asp Gly Val Val Ala Ala Val Lys Ala Asp Arg Tyr Ala Tyr Tyr Thr Ala Phe Phe Asn Asp Phe Tyr Asn Leu Asp Glu Asn Leu Gly Ser Arg Ile Ser Glu Glu Ala Val Arg Asn Ser Trp Asn Thr Ala Ala Arg Gly Gly Ser Phe Ala Ala Ser Ala Ala Pro Ala Thr Trp Tyr Thr Asp Phe Arg Ala Asp Ile Pro Ala Val Asp Val Pro Ala Leu Ile Leu His Gly Thr Ala Asp Arg Ile Leu Pro Ala Glu Gly Thr Ala Arg Pro Phe His Lys Ala Leu Pro Ser Ala Asp Tyr Val Glu Ile Glu Gly Ala Pro His Gly Leu Leu Trp Thr His Ala Glu Glu Val Asn Thr Ala Leu Leu Ala Phe Leu Ala Lys <210> 55 <211> 843 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.

Sequence Listing (2) <400> 55 atgggttaca tcagcgtcgg tcgcgagaac accaccgaca tcgagatcta ctacgaggac 60 cacggcagcg ggcagccggt cgtcctgatc cacggctacc cgctggacgg caacagctgg 120 gagctgcagg cgcgagagct gctcgactcc ggctaccgcg tcatcaccta cgaccgccgc 180 ggcttcggca ggtccagcaa ggtcggcacc ggctacgact acgacacgtt cgccgcggac 240 ctgaacgccg tcctcgagac gctcgacctg cgcgacgtca tcctcgtcgg cttctccatg 300 ggtaccggcg agctcgcccg ttacgtgaag aaccacggga gcgagcgtgt cgccaagctc 360 gcgttcctgg cgtcgctcga gccgttcctc gtggccaccg acgacaaccc cgacggcgtg 420 ccgcagtccg tcttcgacgg catcgtcgag gcggcccgcg acgaccggta cgcctggtac 480 acccagttct tcgaggactt ctacaacctc gacgagacgc tcggctcgcg catcagccag 540 gaggtcgtca cggcgagctg gaacaccgcg accgccagcg cgcccgtcgc ggcctacgcc 600 gtcgtcccga cctggatcga ggacttccgc gccgacgtgg aagcggtccg ggcggcgggc 660 aagcccgcgc tcatcctgca cgggaccgcg gacaacatcc tgcccatcga cgccaccgga 720 cgccgcttcc acgagtcctt cccggaggcc gactacgtcg agatcgacgg cgccccgcac 780 gggctcctgt ggacccacgc cgcggaggtc aacgaggcgc tgctccgctt cgtccgggcc 840 tga 843 <210> 56 <211> 280 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 56 Met Gly Tyr Ile Ser Val Gly Arg Glu Asn Thr Thr Asp Ile Glu Ile Tyr Tyr Glu Asp His Gly Ser Gly Gln Pro Val Val Leu Ile His Gly Tyr Pro Leu Asp Gly Asn Ser Trp Glu Leu Gln Ala Arg Glu Leu Leu Sequence Listing (2) Asp Ser Gly Tyr Arg Val Ile Thr Tyr Asp Arg Arg Gly Phe Gly Arg Ser Ser Lys Val Gly Thr Gly Tyr Asp Tyr Asp Thr Phe Ala Ala Asp Leu Asn Ala Val Leu Glu Thr Leu Asp Leu Arg Asp Val Ile Leu Val Gly Phe Ser Met Gly Thr Gly Glu Leu Ala Arg Tyr Val Lys Asn His Gly Ser Glu Arg Val Ala Lys Leu Ala Phe Leu Ala Ser Leu Glu Pro Phe Leu Val Ala Thr Asp Asp Asn Pro Asp Gly Val Pro Gln Ser Val Phe Asp Gly Ile Val Glu Ala Ala Arg Asp Asp Arg Tyr Ala Trp Tyr Thr Gin Phe Phe Glu Asp Phe Tyr Asn Leu Asp Glu Thr Leu Gly Ser Arg Ile Ser Gln Glu Val Val Thr Ala Ser Trp Asn Thr Ala Thr Ala Ser Ala Pro Val Ala Ala Tyr Ala Vai Val Pro Thr Trp Ile Glu Asp Phe Arg Ala Asp Val Glu Ala Val Arg Ala Ala Gly Lys Pro Ala Leu 210 21'> 220 Ile Leu His Gly Thr Ala Asp Asn Ile Leu Pro Ile Asp Ala Thr Gly Arg Arg Phe His Glu Ser Phe Pro Glu Ala Asp Tyr Val Glu Ile Asp Gly Ala Pro His Gly Leu Leu Trp Thr His Ala Ala Glu Val Asn Glu Sequence Listing (2) Ala Leu Leu Arg Phe Val Arg Ala <210> 57 <211> 846 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 57 atgaccactg tcactactaa ggatggagcc gagatctact acaaggattg gggcgctggg 60 caacctgttg tctttagcca cggctggccc ttgtcgtctg aagcgtgggg gagccaaatg 120 ctgttcctcg cacagcgcgg ctatcgagtc atcgcccacg accggcgagg ccacgggcgc 180 tcaagccaga cctcatccgg caacgatatg aatacctacg cggatgatct tgccgctctg 240 atcgagcacc tcgacctgaa gaacgccgta ctcgtcggcc actctaccgg aggaggcgag 300 gtgactcgct atgtcggccg ctacggaacc aagcgcgtgg acaaagtggt tctgatcgcg 360 gcagtcccgc cactcctgct aaagaccgac gcgaaccccg agggaattcc gatagaggtg 420 ttcgatggct tgcgcgccag tctcgtcaag gatccttcgc aattctggaa agacttcgcc 480 atgatgttct acggcgcaaa tcgttcggga gccaaggtct cgcagggtat cctcgaccag 540 ttctggctgt ggagcattca gggcggtttg aagaatgtct atgaatgcat caaagcattc 600 tccgagaccg acttcaccga ggatctaaag aagttcgatg tgccgacctt gttgctccac 660 ggcgaggatg atcaggtcgt tccagtgatg gagtcgtcca agaaatccgc taaactcatc 720 cggggcgcga aggacatcta ctatccgggt gctccccacg gcattaccgc cactcacccg 780 gatcaaatta acgccgagct gttggagttc ttgaacaaag gcaagagaga ttctcgagcc 840 gcctga 846 <210> 58 <211> 281 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.

Sequence Listing (2) <400> 58 Met Thr Thr Val Thr Thr Lys Asp Gly Ala Glu Ile Tyr Tyr Lys Asp Trp Gly Ala Gly Gln Pro Val Val Phe Ser His Gly Trp Pro Leu Ser Ser Glu Ala Trp Gly Ser Gln Met Leu Phe Leu Ala Gln Arg Gly Tyr Arg Val Ile Ala His Asp Arg Arg Gly His Gly Arg Ser Ser Gln Thr Ser Ser Gly Asn Asp Met Asn Thr Tyr Ala Asp Asp Leu Ala Ala Leu Ile Glu His Leu Asp Leu Lys Asn Ala Val Leu Val Gly His Ser Thr Gly Gly Gly Glu Val Thr Arg Tyr Val Gly Arg Tyr Gly Thr Lys Arg Val Asp Lys Val Val Leu Ile Ala Ala Val Pro Pro Leu Leu Leu Lys Thr Asp Ala Asn Pro Glu Gly Ile Pro Ile Glu Val Phe Asp Gly Leu Arg Ala Ser Leu Val Lys Asp Pro Ser Gln Phe Trp Lys Asp Phe Ala Met Met Phe Tyr Gly Ala Asri Arg Ser Gly Ala Lys Val Ser Gln Gly Ile Leu Asp Gln Phe Trp Leu Trp Ser Ile Gln Gly Gly Leu Lys Asn Val Tyr Glu Cys Ile Lys Ala Phe Ser Glu Thr Asp Phe Thr Glu Asp Sequence Listing (2) Leu Lys Lys Phe Asp Val Pro Thr Leu Leu Leu His Gly Glu Asp Asp Gln Val Val Pro Val Met Glu Ser Ser Lys Lys Ser Ala Lys Leu Ile Arg Gly Ala Lys Asp Ile Tyr Tyr Pro Gly Ala Pro His Gly Ile Thr Ala Thr His Pro Asp Gln Ile Asn Ala Glu Leu Leu Glu Phe Leu Asn Lys Gly Lys Arg Asp Ser Arg Ala Ala <210> 59 <211> 831 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 59 atgagcggtt ttgacaaact tactcttaat gacggcagct atctgtattt caaagactgg 60 gggagcgggc agccggttgt ttttagccac ggctggccgc taacggctga cgcttttgaa 120 gaccagatgc tgtttctggc tgataacggg tttcgggcga tcgcccacga tcgccgcggc 180 catggtcgat cggcccagcc ctgggacggc cacaacatgg atacctatgc tgacgatctg 240 gctcagctga cggcgcatct caacctgcgc gatgcggtgc acgtgggcca ctccaccggc 300 ggtggcgagg tggcgcgcta tatcgggcgc cacgggactg aaagggtggc caaagcggtg 360 cttatcagcg ccgtgacgcc gataatgatc cgcacggatt tcaacccgga cggcgtgccg 420 aaggcggtat ttgacagcat cagggagggc gttatcgagg accgcggcgc gttcttctat 480 gagctgaccg ccgcgttcta tggctacaac cgcgacgggg ccaaagagtc aaaagcggta 540 cgcgaagggt tcgtcgagca ggggctgcag gggtcgatta aagcgcttta tgactgtatc 600 aaagccttct cggaaaccga tctgcgcgag gacctgcgca agatgaccat tccaaccctg 660 gttatccacg gcgatgacga ccagatcgtg cctttcgaaa cctgcggtaa agcggcggcg 720 Sequence Listing (2) caaatattgc ctgacgccag attaaccgtc tacaaaggcg ggtcgcacgg gatttgtacg 780 acccacaagc atcagattaa tcaggattta ctggcgttta ttcgcgaatg a 831 <210> 60 <211> 276 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 60 Met Ser Gly Phe Asp Lys Leu Thr Leu Asn Asp Gly Ser Tyr Leu Tyr Phe Lys Asp Trp Gly Ser Gly Gln Pro Val Val Phe Ser His Gly Trp Pro Leu Thr Ala Asp Ala Phe Glu Asp Gln Met Leu Phe Leu Ala Asp Asn Gly Phe Arg Ala Ile Ala His Asp Arg Arg Gly His Gly Arg Ser Ala Gln Pro Trp Asp Gly His Asn Met Asp Thr Tyr Ala Asp Asp Leu Ala Gln Leu Thr Ala His Leu. Asn Leu Arg Asp Ala Val His Val Gly His Ser Thr Gly Gly Gly Glu Val Ala Arg Tyr Ile Gly Arg His Gly Thr Glu Arg Val Ala Lys Ala. Val Leu Ile Ser Ala Val Thr Pro Ile Met Ile Arg Thr Asp Phe Asn Pro Asp Gly Val Pro Lys Ala Val Phe Asp Ser Ile Arg Glu Gly Val Ile Glu Asp Arg Gly Ala Phe Phe Tyr Sequence Listing (2) Glu Leu Thr Ala Ala Phe Tyr Gly Tyr Asn Arg Asp Gly Ala Lys Glu Ser Lys Ala Val Arg Glu Gly Phe Val Glu Gln Gly Leu Gln Gly Ser Ile Lys Ala Leu Tyr Asp Cys Ile Lys Ala Phe Ser Glu Thr Asp Leu Arg Glu Asp Leu Arg Lys Met Thr Ile Pro Thr Leu Val Ile His Gly Asp Asp Asp Gln Ile Val Pro Phe Glu Thr Cys Gly Lys Ala Ala Ala Gln Ile Leu Pro Asp Ala Arg Leu Thr Val Tyr Lys Gly Gly Ser His Gly Ile Cys Thr Thr His Lys His Gln Ile Asn Gln Asp Leu Leu Ala Phe Ile Arg Glu <210> 61 <211> 837 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 61 atggcattcg taacaactaa agatggcgtt aatatttatt ttaaagactg ggggccgaaa 60 gaggctcagc cgattgtgtt ccaccatggc tggccgttaa gcgctgatga ttgggataac 120 cagatgctgt ttttcctcgc tcaaggattt cgcgtcatcg ctatcgatcg ccgcgggcat 180 gggcgttccg accaggtaag tgaaggtcat gatatggacc actatgcggc tgatgcttcc 240 gctgtagtgg aaagtctgga tttgcataac gctgttcatg ttggtcattc aaccggcggc 300 ggtcaggtcg cgcgctatgt ggctaaatat ggtcaaccgc agggtcgggt agccaaagca 360 Sequence Listing (2) gtattaatta gttccgtacc gccgt.taatg gtgaaaaccg acaataaccc cggcggtacg 420 cccatcgaag tgtttgacgg tttccgcaat gcgcttgcgg ctaatcgctc gcagttctat 480 ctcgatgttg ccagtggccc gttctatggt tttaaccgag atggcgcaga ggtttcgcaa 540 ggtaccattc aaaactggtg gcgtcagggg atgaccggta gcgcgaaagc ccattatgaa 600 gggattaagg ccttctctga aaccgaccag acagaagatc tgaaagcgat tacggtgccg 660 gtgttggtaa tgcagggtga cgacgaccag gtcgttcctt ataaaaatgc cgccattctg 720 caggataaac tgttagcgaa tagcgagctg aaaatttatc cgggcttacc gcatggtatg 780 cacacgtcac atgcggatgt gattaacgcc gatctgctgg ctttcattcg ttcataa 837 <210> 62 <211> 278 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 62 Met Ala Phe Val Thr Thr Lys Asp Gly Val Asn Ile Tyr Phe Lys Asp Trp Gly Pro Lys Glu Ala Gln Pro Ile Val Phe His His Gly Trp Pro Leu Ser Ala Asp Asp Trp Asp Asn Gln Met Leu Phe Phe Leu Ala Gln Gly Phe Arg Val Ile Ala Ile Asp Arg Arg Gly His Gly Arg Ser Asp Gln Val Ser Glu Gly His Asp Met Asp His Tyr Ala Ala Asp Ala Ser Ala Val Val Glu Ser Leu Asp Leu His Asn Ala Val His Val Gly His Ser Thr Gly Gly Gly Gln Val Ala Arg Tyr Val Ala Lys Tyr Gly Gln Sequence Listing (2) Pro Gln Gly Arg Val Ala Lys Ala Val Leu Ile Ser Ser Val Pro Pro Leu Met Val Lys Thr Asp Asn Asn Pro Gly Gly Thr Pro Ile Glu Val Phe Asp Gly Phe Arg Asn Ala Leu Ala Ala Asn Arg Ser Gln Phe Tyr Leu Asp Val Ala Ser Gly Pro Phe Tyr Gly Phe Asn Arg Asp Gly Ala Glu Val Ser Gln Gly Thr Ile Gln Asn Trp Trp Arg Gln Gly Met Thr Gly Ser Ala Lys Ala His Tyr Glu Gly Ile Lys Ala Phe Ser Glu Thr Asp Gln Thr Glu Asp Leu Lys Ala Ile Thr Val Pro Val Leu Val Met Gln Gly Asp Asp Asp Gln Val Val Pro Tyr Lys Asn Ala Ala Ile Leu Gln Asp Lys Leu Leu Ala Asn Ser Glu Leu Lys Ile Tyr Pro Gly Leu Pro His Gly Met His Thr Ser His Ala Asp Val Ile Asn Ala Asp Leu Leu Ala Phe Ile Arg Ser <210> 63 <211> 822 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 63 Sequence Listing (2) atgagctttt tcaccaccac cgacgggacg cgcctctttt acaaggactg gggaacgggc 60 caaccgatcc tgttttcgca tggctggccg ctgtcctccg acgcctggga tcagcagatg 120 ctgttcttcg gccagcaggg ttttcgcgtc atcgcccatg accgccgcag ccatggccgc 180 tccgaccaga cctgggatgg caacaacatg gaccgctatg ctgacgatct ggcggagctg 240 atcgaggcgc tggacctgaa ggatatcgtc atgatcggcc attcgaccgg aggcggcgag 300 gtggcccact atctcggacg tcacggcacg gcgcgcgtcg ccaaggtcgt gctggtcggc 360 gcggttccgc cgctgatgct caagaccgaa gccaatccgc acggcacgcc gatcgaggcc 420 ttcgacggca ttcgtgacgc gacggcgaag aaccgctcgc agttctacta tgacctctcc 480 atgcccttct acggcttcaa ccgcgatggt gtcgccgcca atgacggcct gcgggaaagc 540 ttccggcaga tcggcctgca gggcggcatc aaggggcagt atgactgcat ccgcgagttt 600 tccgaggtcg actacactga ggacctgcgc cgcatcgatc gcccgacgct gatcatccat 660 ggcgacgacg accagatcgt gccgatcaag gcctcggctc atcgcgccgc cgagatcgtg 720 ccgaatgcga ccctcacgat ctaccaaggc ggctcgcatg gcctcgccca gaccgaggcg 780 gaccgcttca acgccgacgt cctggccttc atcagggtct ga 822 <210> 64 <211> 273 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 64 Met Ser Phe Phe Thr Thr Thr Asp Gly Thr Arg Leu Phe Tyr Lys Asp Trp Gly Thr Gly Gln Pro Ile Leu Phe Ser His Gly Trp Pro Leu Ser Ser Asp Ala Trp Asp Gln Gln. Met Leu Phe Phe Gly Gln Gln Gly Phe Arg Val Ile Ala His Asp Arg Arg Ser His Gly Arg Ser Asp Gln Thr Sequence Listing (2) Trp Asp Gly Asn Asn Met Asp Arg Tyr Ala Asp Asp Leu Ala Glu Leu Ile Glu Ala Leu Asp Leu Lys Asp Ile Val Met Ile Gly His Ser Thr Gly Gly Gly Glu Val Ala His Tyr Leu Gly Arg His Gly Thr Ala Arg Val Ala Lys Val Val Leu Val Gly Ala Val Pro Pro Leu Met Leu Lys Thr Glu Ala Asn Pro His Gly Thr Pro Ile Glu Ala Phe Asp Gly Ile Arg Asp Ala Thr Ala Lys Asn Arg Ser Gln Phe Tyr Tyr Asp Leu Ser Met Pro Phe Tyr Gly Phe Asn Arg Asp Gly Val Ala Ala Asn Asp Gly Leu Arg Glu Ser Phe Arg Gln Ile Gly Leu Gln Gly Gly Ile Lys Gly Gln Tyr Asp Cys Ile Arg Glu Phe Ser Glu Val Asp Tyr Thr Glu Asp Leu Arg Arg Ile Asp Arg Pro Thr Leu Ile Ile His Gly Asp Asp Asp Gln Ile Val Pro Ile Lys Ala Ser Ala His Arg Ala Ala Glu Ile Val Pro Asn Ala Thr Leu Thr Ile Tyr Gln Gly Gly Ser His Gly Leu Ala Gln Thr Glu Ala Asp Arg Phe Asn Ala Asp Val Leu Ala Phe Ile Arg Val Sequence Listing (2) <210> 65 <211> 831 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 65 atgggttacg taacgaccag cgatggcgtc gagattttct acaaggattg gggccccaaa 60 aacggcaagg ttatctactt ccaccacggc tggccgctgt ccagcgatga ctgggatgcc 120 cagatgctgt tcttcgtgaa caaagggttc cgcgtggtgg cgcacgatcg ccgcggccac 180 ggccgctcca gccaggtgtc ggaagggcac gacatggacc actacgccga tgacgtcgcc 240 gcggtggtaa aacacctcgg cgtgcagggc gcgatgcacg tgggccactc gacgggcggc 300 ggcgaagtgg tgcgctatat tgtgcgccac ggcgaggaca aggtgtccaa agcggtgctg 360 atcagcgcgg tgccgccgct gatggtaaaa accgacgcca atccgcaggg tacccccaaa 420 gcggtgttcg atgatttcca ggcgcagctg gccgccaacc gggcgcagtt ctattacgac 480 gtgccggccg gcccgttcta cggctataac cgcccgggcg ccaaaccgtc tgaggcgatc 540 atctggaact ggtggcgcca gggcatgatg ggcggcgcca aggcgcacta cgacggcatc 600 gtcgccttct cgcagaccga tttcaccgaa gatctcaaga agatcgccat tccggtgctg 660 gtgatccacg gcgacgacga tcaggtggtg ccttatcagg attccggcgt gctgtcggcc 720 aagctggtgc agaacggcac gctccatacc tataagggcg cgccgcacgg catcccgacc 780 acccacgccg atcaggtcaa cgccgatctg ctggcgttcg tgaacagcta a 831 <210> 66 <211> 276 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 66 Met Gly Tyr Val Thr Thr Ser Asp Gly Val Glu Ile Phe Tyr Lys Asp Sequence Listing (2) Trp Gly Pro Lys Asn Gly Lys Val Ile Tyr Phe His His Gly Trp Pro Leu Ser Ser Asp Asp Trp Asp Ala Gln Met Leu Phe Phe Val Asn Lys Gly Phe Arg Val Val Ala His Asp Arg Arg Gly His Gly Arg Ser Ser Gln Val Ser Glu Gly His Asp Met Asp His Tyr Ala Asp Asp Val Ala Ala Val Val Lys His Leu Gly Val Gln Gly Ala Met His Val Gly His Ser Thr Gly Gly Gly Glu Val Val Arg Tyr Ile Val Arg His Gly Glu Asp Lys Val Ser Lys Ala Val Leu Ile Ser Ala Val Pro Pro Leu Met Val Lys Thr Asp Ala Asn Pro Gln Gly Thr Pro Lys Ala Val Phe Asp Asp Phe Gln Ala Gln Leu Ala Ala Asn Arg Ala Gln Phe Tyr Tyr Asp Val Pro Ala Gly Pro Phe Tyr Gly Tyr Asn Arg Pro Gly Ala Lys Pro Ser Glu Ala Ile Ile Trp Asn Trp Trp Arg Gln Gly Met Met Gly Gly Ala Lys Ala His Tyr Asp Gly Ile Val Ala Phe Ser Gln Thr Asp Phe Thr Glu Asp Leu Lys Lys Ile Ala Ile Pro Val Leu Val Ile His Gly Asp Asp Asp Gln Val Val Pro Tyr Gin Asp Ser Gly Val Leu Ser Ala Sequence Listing (2) Lys Leu Val Gln Asn Gly Thr Leu His Thr Tyr Lys Gly Ala Pro His Gly Ile Pro Thr Thr His Ala. Asp Gln Val Asn Ala Asp Leu Leu Ala Phe Val Asn Ser <210> 67 <211> 681 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 67 atggcattcg taacaacgaa agacggcgtc aatatttact acaaagactg gggcccaaaa 60 gaggctcaac ccattgtttt ccaccacggc tggccgctaa gtgccgatga ctgggataac 120 cagatgctct tcttcctcgc agaaggctat cgcgttatcg ctatcgatcg tcgtggtcac 180 gggcgttcgg accaggtgag tgaaggccac gatatggatc actatgcggc cgacgcgtcg 240 gccgtggttg aaagcctgga cctgcgcaat gccgtgcacg tcggtcactc caccggcggc 300 ggacaggtcg cccgctatgt tgcgcagtac gggcaaccgc aggggcgcgt cgccaaagcg 360 gtgctcgtca gcgccgtccc gccgctgatg gtcaaaacgg acagtaaccc cggcggaacg 420 cccatcgagg ttttcgacgg cttccgcaaa gcgctggcgg ctaatcgcgc ccagttttac 480 ctcgacgtcg ccagcggccc tttctacggc tttaatcggg atggcgcgga cgtttcacag 540 ggcacgattc aaaactggtg gcgtcaggga atgatcggca gcgccaaggc ccactacgag 600 gggattaagg cattttcaga gaccgaccag acggaggatc taaaaaccat cacagttccc 660 gttctggtgt tgcagggctg a 681 <210> 68 <211> 226 <212> PRT
<213> Unknown Sequence Listing (2) <220>
<223> Obtained from an environmental sample.
<400> 68 Met Ala Phe Val Thr Thr Lys Asp Gly Val Asn Ile Tyr Tyr Lys Asp Trp Gly Pro Lys Glu Ala Gln Pro Ile Val Phe His His Gly Trp Pro Leu Ser Ala Asp Asp Trp Asp Asn Gln Met Leu Phe Phe Leu Ala Glu Gly Tyr Arg Val Ile Ala Ile Asp Arg Arg Gly His Gly Arg Ser Asp Gln Val Ser Glu Gly His Asp Met Asp His Tyr Ala Ala Asp Ala Ser Ala Val Val Glu Ser Leu Asp Leu Arg Asn Ala Val His Val Gly His Ser Thr Gly Gly Gly Gln Val Ala Arg Tyr Val Ala Gln Tyr Gly Gln Pro Gln Gly Arg Val Ala Lys Ala Val Leu Val Ser Ala Val Pro Pro Leu Met Val Lys Thr Asp Ser Asn Pro Gly Gly Thr Pro Ile Glu Val Phe Asp Gly Phe Arg Lys Ala Leu Ala Ala Asn Arg Ala Gln Phe Tyr Leu Asp Val Ala Ser Gly Pro Phe Tyr Gly Phe Asn Arg Asp Gly Ala Asp Val Ser Gln Gly Thr Ile Gln Asn Trp Trp Arg Gln Gly Met Ile Gly Ser Ala Lys Ala His Tyr Glu Gly Ile Lys Ala Phe Ser Glu Thr Sequence Listing (2) Asp Gln Thr Glu Asp Leu Lys Thr Ile Thr Val Pro Val Leu Val Leu Gln Gly <210> 69 <211> 921 <212> DNA
<213> Artificial <220>
<223> Artificially modified (mutated) dehalogenase <400> 69 atgtcagaaa tcggtacagg cttccccttc gacccccatt atgtggaagt cctgggcgag 60 cgtatgcact acgtcgatgt tggaccgcgg gatggcacgc ctgtgctgtt cctgcacggt 120 aacccgacct cgtcctacct gtggcgcaac atcatcccgc atgtagcacc gagtcatcgg 180 tgcattgctc cagacctgat cgggatggga aaatcggaca aaccagacct cggttatttc 240 ttcgacgacc acgtccgcta cctcgatgcc ttcatcgaag ccttgggttt ggaagaggtc 300 gtcttggtca tccacgactg gggctcagct ctcggattcc actgggccaa gcgcaatccg 360 gaacgggtca aaggtattgc atgtatggaa ttcatccggt ctatcccgac gtgggacgaa 420 tggccggaat tcgcccgtga gacct.tccag gccttccgga ccgccgacgt cggccgaga.g 480 ttgatcatcg atcagaacgc tttcatcgag catgtgctcc cgaaatacgt cgtccgtccg 540 cttacggagg tcgagatgga ccactatcgc gagcccttcc tcaagcctgc tgaccgagag 600 ccactgtggc gattccccaa cgagctcccc atcgccggtg agcccgcgaa catcgtcgcg 660 ctcgtcgagg catacatgaa ctggctgcac cagtcacctg tcccgaagtt gttgttctgg 720 ggcacacccg gcctactgat ccccccggcc gaagcctcga gacttgccga aagcctcccc 780 aactgcaaga cagtggacat cggcccggga ctgcacttcc tccaggaaga caacccggac 840 cttatcggca gtgagatcgc gcgct.ggctc gccggactcg cgagcggcct cggcgactac 900 catcatcatc atcatcatta a 921 <210> 70 Sequence Listing (2) <211> 306 <212> PRT
<213> Artificial <220>
<223> Artificially modified (mutated) dehalogenase <400> 70 Met Ser Glu Ile Gly Thr Gly Phe Pro Phe Asp Pro His Tyr Val Glu Val Leu Gly Glu Arg Met His Tyr Val Asp Val Gly Pro Arg Asp Gly Thr Pro Val Leu Phe Leu His Gly Asn Pro Thr Ser Ser Tyr Leu Trp Arg Asn Ile Ile Pro His Val Ala Pro Ser His Arg Cys Ile Ala Pro Asp Leu Ile Gly Met Gly Lys Ser Asp Lys Pro Asp Leu Gly Tyr Phe Phe Asp Asp His Val Arg Tyr Leu Asp Ala Phe Ile Glu Ala Leu Gly Leu Glu Glu Val Val Leu Val. Ile His Asp Trp Gly Ser Ala Leu Gly Phe His Trp Ala Lys Arg Asn Pro Glu Arg Val Lys Gly Ile Ala Cys Met Glu Phe Ile Arg Ser Ile Pro Thr Trp Asp Glu Trp Pro Glu Phe Ala Arg Glu Thr Phe Gln Ala Phe Arg Thr Ala Asp Val Gly Arg Glu Leu Ile Ile Asp Gln Asn Ala Phe Ile Glu His Val Leu Pro Lys Tyr Val Val Arg Pro Leu Thr Glu Val Glu Met Asp His Tyr Arg Glu Pro Sequence Listing (2) Phe Leu Lys Pro Ala Asp Arg Glu Pro Leu Trp Arg Phe Pro Asn Glu Leu Pro Ile Ala Gly Glu Pro Ala Asn Ile Val Ala Leu Val Glu Ala Tyr Met Asn Trp Leu His Gln. Ser Pro Val Pro Lys Leu Leu Phe Trp Gly Thr Pro Gly Leu Leu Ile Pro Pro Ala Glu Ala Ser Arg Leu Ala Glu Ser Leu Pro Asn Cys Lys Thr Val Asp Ile Gly Pro Gly Leu His Phe Leu Gln Glu Asp Asn Pro Asp Leu Ile Gly Ser Glu Ile Ala Arg Trp Leu Ala Gly Leu Ala Ser Gly Leu Gly Asp Tyr His His His His His His <210> 71 <211> 942 <212> DNA
<213> Loligo vulgaris <400> 71 atggaaattc cagttatcga acctcttttc acaaaagtga ccgaagatat accaggtgca 60 gagggtcccg tttttgacaa aaatggcgat ttttatatcg tggcccccga agttgaagtt 120 aacggaaaac cggcgggaga aattctacga atcgatttga aaacaggaaa gaaaactgtg 180 atctgcaaac cagaagttaa tggttatgga ggaattcctg ctggctgcca atgtgatcga 240 gatgccaacc agctgtttgt ggccgacatg agactcggct tgttggtcgt gcaaactgat 300 gggacctttg aagagattgc caaaaaagac tctgaaggta gaagaatgca gggatgcaat 360 gattgcgcat ttgattatga aggtaacttg tggatcactg caccagctgg ggaagtcgca 420 Sequence Listing (2) cctgcagact acacccgttc aatgcaggaa aaatttggca gtatttactg cttcacaaca 480 gatggtcaaa tgattcaagt ggatactgct ttccagtttc caaatggtat tgctgttcgt 540 cacatgaacg atggccgtcc ttaccaacta attgtggctg aaactccaac caagaaactc 600 tggagttatg atatcaaagg tccagcaaag attgaaaaca agaaagtgtg gggtcacatc 660 ccaggtactc atgaaggtgg tgctgatgga atggattttg atgaagacaa taaccttttg 720 gtagccaact gggggagctc acacatcgaa gtgttcggcc cagatggggg acagcctaaa 780 atgagaatcc gttgcccatt tgaaaaaccc agcaacttgc atttcaagcc ccagaccaaa 840 accatttttg tcacggaaca cgagaacaat gctgtctgga agtttgaatg gcaaagaaat 900 ggcaaaaaac agtattgtga gacgttaaaa tttggaatat tt 942 <210> 72 <211> 314 <212> PRT
<213> Loligo vulgaris <400> 72 Met Glu Ile Pro Val Ile Glu Pro Leu Phe Thr Lys Val Thr Glu Asp Ile Pro Gly Ala Glu Gly Pro Val Phe Asp Lys Asn Gly Asp Phe Tyr Ile Val Ala Pro Glu Val Glu. Val Asn Gly Lys Pro Ala Gly Glu Ile Leu Arg Ile Asp Leu Lys Thr Gly Lys Lys Thr Val Ile Cys Lys Pro Glu Val Asn Gly Tyr Gly Gly Ile Pro Ala Gly Cys Gln Cys Asp Arg Asp Ala Asn Gln Leu Phe Val Ala Asp Met Arg Leu Gly Leu Leu Val Val Gln Thr Asp Gly Thr Phe Glu Glu Ile Ala Lys Lys Asp Ser Glu Sequence Listing (2) Gly Arg Arg Met Gln Gly Cys Asn Asp Cys Ala Phe Asp Tyr Glu Gly Asn Leu Trp Ile Thr Ala Pro Ala Gly Glu Val Ala Pro Ala Asp Tyr Thr Arg Ser Met Gln Glu Lys Phe Gly Ser Ile Tyr Cys Phe Thr Thr Asp Gly Gln Met Ile Gln Val. Asp Thr Ala Phe Gln Phe Pro Asn Gly Ile Ala Val Arg His Met Asn Asp Gly Arg Pro Tyr Gln Leu Ile Val Ala Glu Thr Pro Thr Lys Lys Leu Trp Ser Tyr Asp Ile Lys Gly Pro Ala Lys Ile Glu Asn Lys Lys Val Trp Gly His Ile Pro Gly Thr His Glu Gly Gly Ala Asp Gly Met. Asp Phe Asp Glu Asp Asn Asn Leu Leu Val Ala Asn Trp Gly Ser Ser His Ile Glu Val Phe Gly Pro Asp Gly Gly Gln Pro Lys Met Arg Ile Arg Cys Pro Phe Glu Lys Pro Ser Asn Leu His Phe Lys Pro Gln Thr Lys Thr Ile Phe Val Thr Glu His Glu Asn Asn Ala Val Trp Lys Phe Glu Trp Gln Arg Asn Gly Lys Lys Gln Tyr Cys Glu Thr Leu Lys Phe Gly Ile Phe <210> 73 Sequence Listing (2) <211> 876 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 73 atgaatgcat tctggaaccg attggcaggc gcaacgctga tgctcgcact gatgtccacg 60 gcctgggcag gtgtggaatg gccgcgagtg gccgaatcct ttgatggtgt gccgatatct 120 tatgaaatta ctggggaggg cgagcccgct cttgtattca tccatggctg gagttgcgat 180 gcccgctact ggcgagccca ggtgccgcac ttctcaagcg atcaccaggt gatcagtatc 240 gatctggccg gccatggaca ctccgggctg gaacgcgaga actacaccat ggccgccttc 300 ggcgcggacg tcaaggccgt gctcgatgat gccgacatcc aggaagcgat tctgattgga 360 cactccatgg gcgggccggt cagcgtggct gcggccaaat tgatgcccga acgggtgatc 420 ggcattgtcg gggtcgatac ttttcagaat gtcggccaga gcatgagcga agaagaagtc 480 gaggcctgga tggggccatt gcgtgcggat ttccgccatg gtgccagtca gttcgtgcca 540 ggcatgttca tcgaggacac tgatgccgaa ttgcgtgact gggtcgtggc agacatgtcg 600 gccgcgccgc ctgaagtggc gctcagtgcc atgcagaaca tgttggccga tatcacaagt 660 ggtcaggcac tctcggcgtt ctctggtctg gatgttccag tggtggccat caacgccgat 720 atctggccaa gtgatcccga ggccaatcgg aggcaccagc cccggtttga tgccgtgatc 780 atggaaaata ccgaccattt cctgcacatg gccgagcccg aggccttcaa tcgtgaattg 840 cgagcggtta tcgccaggtt gaccgagtca cgctga 876 <210> 74 <211> 291 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 74 Met Asn Ala Phe Trp Asn Arg Leu Ala Gly Ala Thr Leu Met Leu Ala Leu Met Ser Thr Ala Trp Ala Gly Val Glu Trp Pro Arg Val Ala Glu Sequence Listing (2) Ser Phe Asp Gly Val Pro Ile Ser Tyr Glu Ile Thr Gly Glu Gly Glu Pro Ala Leu Val Phe Ile His Gly Trp Ser Cys Asp Ala Arg Tyr Trp Arg Ala Gln Val Pro His Phe Ser Ser Asp His Gln Val Ile Ser Ile Asp Leu Ala Gly His Gly His Ser Gly Leu Glu Arg Glu Asn Tyr Thr Met Ala Ala Phe Gly Ala Asp Val Lys Ala Val Leu Asp Asp Ala Asp Ile Gln Glu Ala Ile Leu Ile Gly His Ser Met Gly Gly Pro Val Ser Val Ala Ala Ala Lys Leu Met Pro Glu Arg Val Ile Gly Ile Val Gly Val Asp Thr Phe Gln Asn Val Gly Gln Ser Met Ser Glu Glu Glu Val Glu Ala Trp Met Gly Pro Leu Arg Ala Asp Phe Arg His Gly Ala Ser Gln Phe Val Pro Gly Met Phe Ile Glu Asp Thr Asp Ala Glu Leu Arg Asp Trp Val Val Ala Asp Met Ser Ala Ala Pro Pro Glu Val Ala Leu Ser Ala Met Gin Asn Met Leu Ala Asp Ile Thr Ser Gly Gin Ala Leu Ser Ala Phe Ser Gly Leu Asp Val Pro Val Val Ala Ile Asn Ala Asp Sequence Listing (2) Ile Trp Pro Ser Asp Pro Glu Ala Asn Arg Arg His Gln Pro Arg Phe Asp Ala Val Ile Met Glu Asn Thr Asp His Phe Leu His Met Ala Glu Pro Glu Ala Phe Asn Arg Glu Leu Arg Ala Val Ile Ala Arg Leu Thr Glu Ser Arg <210> 75 <211> 876 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 75 atgagttcag cgttgttccg attgatgctt gtggccgtgg tgctggttgg gtccggcctt 60 gtccgggcag agattcaatg gccgcgcatg gccgaatcat tcgatggcct acccatttcc 120 tatgaggtaa cggggcaggg tgatcccaca ctggtgttta tccacggctg gagctgcgat 180 gcccgttact ggcgtgccca gatctcgcac tttgcagccg atcaccaggt gatcagtatc 240 gaccttgcag gccacggcca ttccgggctg gggcgcgaga gctataccat ggccgcgttc 300 ggccgcgacg tgaaagcagt cgtcgaagat gcgggtgcgg atcaggtcat cctgatcggg 360 cattccatgg gcggtccggt cagcgttgca gctgccgggc tgatgccggg tcgggtaatc 420 ggcgttgtcg gtgtcgacac gttccagaac gtcggtcaga ccatgagcca ggaagaggcc 480 gaagcctgga ttgcgccttt gcgagaggat ttccgccagg gtgcagggca gttcgtgccg 540 cagatgttca tcgaggctac cgatgccgaa ctcagagact gggtgattgc cgacatgtcg 600 gccgcaccgc cggaagtcgc tatcagcgcc atgagcaata tgctcaccga catgatcagt 660 ggtcaggcgc tttctgcgtt ctccgctctc gaagtaccgg tagtggccat caatgcagat 720 atctggccga ccgatgtcga ggccaaccag aagcatcagc cccgcttcga ggcggtgatc 780 atggatgaca ccgatcactt tctgcaaatg gccgaacctg aagccttcaa tcgcgagctg 840 Sequence Listing (2) gagcaggtga tcgcgaggct tttggagtca cactga 876 <210> 76 <211> 291 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 76 Met Ser Ser Ala Leu Phe Arg Leu Met Leu Val Ala Val Val Leu Val Gly Ser Gly Leu Val Arg Ala Glu Ile Gln Trp Pro Arg Met Ala Glu Ser Phe Asp Gly Leu Pro Ile Ser Tyr Glu Val Thr Gly Gln Gly Asp Pro Thr Leu Val Phe Ile His Gly Trp Ser Cys Asp Ala Arg Tyr Trp Arg Ala Gln Ile Ser His Phe Ala Ala Asp His Gln Val Ile Ser Ile Asp Leu Ala Gly His Gly His Ser Gly Leu Gly Arg Glu Ser Tyr Thr Met Ala Ala Phe Gly Arg Asp Val Lys Ala Val Val Glu Asp Ala Gly Ala Asp Gln Val Ile Leu Ile Gly His Ser Met Gly Gly Pro Val Ser Val Ala Ala Ala Gly Leu Met Pro Gly Arg Val Ile Gly Val Val Gly Val Asp Thr Phe Gln Asn Val. Gly Gln Thr Met Ser Gln Glu Glu Ala Glu Ala Trp Ile Ala Pro Leu Arg Glu Asp Phe Arg Gln Gly Ala Gly Sequence Listing (2) Gln Phe Val Pro Gln Met Phe Ile Glu Ala Thr Asp Ala Glu Leu Arg Asp Trp Val Ile Ala Asp Met Ser Ala Ala Pro Pro Glu Val Ala Ile Ser Ala Met Ser Asn Met Leu Thr Asp Met Ile Ser Gly Gln Ala Leu Ser Ala Phe Ser Ala Leu Glu Val Pro Val Val Ala Ile Asn Ala Asp Ile Trp Pro Thr Asp Val Glu Ala Asn Gln Lys His Gln Pro Arg Phe Glu Ala Val Ile Met Asp Asp Thr Asp His Phe Leu Gln Met Ala Glu Pro Glu Ala Phe Asn Arg Glu Leu Glu Gln Val Ile Ala Arg Leu Leu Glu Ser His <210> 77 <211> 876 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 77 atgaatgcat tctggaaccg attggcaggc gcaacgctga tgctcgcact gatgtccacg 60 gcctgggcag gtgtggaatg gccgcgagtg gccgaatcct ttgatggtgt gccgatatct 120 tatgaaatta ctggggaggg cgagcccgct cttgtattca tccatggctg gagttgcgat 180 gcccgctact ggcgagccca ggtgccgcac ttctcaagcg atcaccaggt gatcagtatc 240 gatctggccg gccatggaca ctccgggctg gaacgcgaga actacaccat ggccgccttc 300 Sequence Listing (2) ggcgcggacg tcaaggccgt gctcgatgat gccgacatcc aggaagcgat tctgattgga 360 cactccatgg gcgggccggt cagcgtggct gcggccaaat tgatgcccga acgggtgatc 420 ggcattgtcg gggtcgatac ttttcagaat gtcggccaga gcatgagcga agaagaagtc 480 gaggcctgga tggggccatt gcgtgcggat ttccgccatg gtgccagtca gttcgtgcca 540 ggcatgttca tcgaggacac tgatgccgaa ttgcgtgact gggtcgtggc agacatgtcg 600 gccgcgccgc ctgaagtggc gctcagtgcc atgcagaaca tgttggccga tatcacaagt 660 ggtcaggcac tctcggcgtt ctctggtctg gatgttccag tggtggccat caacgccgat 720 atctggccaa gtgatcccga ggccaatcgg aggcaccagc cccggtttga tgccgtgatc 780 atggaaaata ccgaccattt cctgcacatg gccgagcccg aggccttcaa tcgtgaattg 840 cgagcggtta tcgccaggtt gaccgagtca cgctga 876 <210> 78 <211> 291 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 78 Met Asn Ala Phe Trp Asn Arg Leu Ala Gly Ala Thr Leu Met Leu Ala Leu Met Ser Thr Ala Trp Ala Gly Val Glu Trp Pro Arg Val Ala Glu Ser Phe Asp Gly Val Pro Ile Ser Tyr Glu Ile Thr Gly Glu Gly Glu Pro Ala Leu Val Phe Ile His Gly Trp Ser Cys Asp Ala Arg Tyr Trp Arg Ala Gln Val Pro His Phe Ser Ser Asp His Gln Val Ile Ser Ile Asp Leu Ala Gly His Gly His Ser Gly Leu Glu Arg Glu Asn Tyr Thr Sequence Listing (2) Met Ala Ala Phe Gly Ala Asp Val Lys Ala Val Leu Asp Asp Ala Asp Ile Gln Glu Ala Ile Leu Ile Gly His Ser Met Gly Gly Pro Val Ser Val Ala Ala Ala Lys Leu Met Pro Glu Arg Val Ile Gly Ile Val Gly Val Asp Thr Phe Gln Asn Val Gly Gln Ser Met Ser Glu Glu Glu Val Glu Ala Trp Met Gly Pro Leu Arg Ala Asp Phe Arg His Gly Ala Ser Gln Phe Val Pro Gly Met Phe Ile Glu Asp Thr Asp Ala Glu Leu Arg Asp Trp Val Val Ala Asp Met Ser Ala Ala Pro Pro Glu Val Ala Leu Ser Ala Met Gln Asn Met Leu. Ala Asp Ile Thr Ser Gly Gln Ala Leu Ser Ala Phe Ser Gly Leu Asp Val Pro Val Val Ala Ile Asn Ala Asp Ile Trp Pro Ser Asp Pro Glu Ala Asn Arg Arg His Gln Pro Arg Phe Asp Ala Val Ile Met Glu Asn Thr Asp His Phe Leu His Met Ala Glu Pro Glu Ala Phe Asn Arg Glu Leu Arg Ala Val Ile Ala Arg Leu Thr Glu Ser Arg <210> 79 Sequence Listing (2) <211> 948 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 79 ttgctccggc caggcgggta tgcgatcatg aaaggctttc gtggaaactc gatcgcaact 60 gggagtggga atatgagttc agcgttgttc cgattgatgc ttgtggccgt ggtgctggtt 120 gggtccggcc ttgtccgggc agagattcaa tggccgcgca tggccgaatc attcgatggc 180 ctacccattt cctatgaggt aacggggcag ggtgatccca cactggtgtt tatccacggc 240 tggagctgcg atgcccgtta ctggcgtgcc cagatctcgc actttgcagc cgatcaccag 300 gtgatcagta tcgaccttgc aggccacggc cattccgggc tggggcgcga gagctatacc 360 atggccgcgt tcggccgcga cgtgaaagca gtcgtcgaag atgcgggtgc ggatcaggtc 420 atcctgatcg ggcattccat gggcggtccg gtcagcgttg cagctgccgg gctgatgccg 480 ggtcgggtaa tcggcgttgt cggtgtcgac acgttccaga acgtcggtca gaccatgagc 540 caggaagagg ccgaagcctg gattgcgcct ttgcgagagg atttccgcca gggtgcaggg 600 cagttcgtgc cgcagatgtt catcgaggct accgatgccg aactcagaga ctgggtgatt 660 gccgacatgt cggccgcacc gccggaagtc gctatcagcg ccatgagcaa tatgctcacc 720 gacatgatca gtggtcaggc gctttctgcg ttctccgctc tcgaagtacc ggtagtggcc 780 atcaatgcag atatctggcc gaccgatgtc gaggccaacc agaagcatca gccccgcttc 840 gaggcggtga tcatggatga caccgatcac tttctgcaaa tggccgaacc tgaagccttc 900 aatcgcgagc tggagcaggt gatcgcgagg cttttggagt cacactga 948 <210> 80 <211> 315 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 80 Met Leu Arg Pro Gly Gly Tyr Ala Ile Met Lys Gly Phe Arg Gly Asn Sequence Listing (2) Ser Ile Ala Thr Gly Ser Gly Asn Met Ser Ser Ala Leu Phe Arg Leu Met Leu Val Ala Val Val Leu Val Gly Ser Gly Leu Val Arg Ala Glu Ile Gln Trp Pro Arg Met Ala Glu Ser Phe Asp Gly Leu Pro Ile Ser Tyr Glu Val Thr Gly Gln Gly Asp Pro Thr Leu Val Phe Ile His Gly Trp Ser Cys Asp Ala Arg Tyr Trp Arg Ala Gln Ile Ser His Phe Ala Ala Asp His Gln Val Ile Ser Ile Asp Leu Ala Gly His Gly His Ser Gly Leu Gly Arg Glu Ser Tyr Thr Met Ala Ala Phe Gly Arg Asp Val Lys Ala Val Val Glu Asp Ala. Gly Ala Asp Gln Val Ile Leu Ile Gly His Ser Met Gly Gly Pro Val. Ser Val Ala Ala Ala Gly Leu Met Pro Gly Arg Val Ile Gly Val Val Gly Val Asp Thr Phe Gln Asn Val Gly Gln Thr Met Ser Gln Glu Glu Ala Glu Ala Trp Ile Ala Pro Leu Arg Glu Asp Phe Arg Gln Gly Ala Gly Gln Phe Val Pro Gln Met Phe Ile Glu Ala Thr Asp Ala Glu Leu Arg Asp Trp Val Ile Ala Asp Met Ser Ala Ala Pro Pro Glu Val Ala Ile Ser Ala Met Ser Asn Met Leu Thr Sequence Listing (2) Asp Met Ile Ser Gly Gln Ala Leu Ser Ala Phe Ser Ala Leu Glu Val Pro Val Val Ala Ile Asn Ala. Asp Ile Trp Pro Thr Asp Val Glu Ala Asn Gln Lys His Gln Pro Arg Phe Glu Ala Val Ile Met Asp Asp Thr Asp His Phe Leu Gln Met Ala Glu Pro Glu Ala Phe Asn Arg Glu Leu Glu Gln Val Ile Ala Arg Leu Leu Glu Ser His <210> 81 <211> 876 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 81 atgaatgcat tctggaaccg attggcaggc gcaacgctga tgctcgcact gatgtccacg 60 gcctgggcag gtgtggaatg gccgcgagtg gccgaatcct ttgatggtgt gccgatatct 120 tatgaaatta ctggggaggg cgagcccgct cttgtattca tccatggctg gagttgcgat 180 gcccgctact ggcgagccca ggtgccgcac ttctcaagcg atcaccaggt gatcagtatc 240 gatctggccg gccatggaca ctccgggctg gaacgcgaga actacaccat ggccgccttc 300 ggcgcggacg tcaaggccgt gctcqatgat gccgacatcc aggaagcgat tctgattgga 360 cactccatgg gcgggccggt cagcgtggct gcggccaaat tgatgcccga acgggtgatc 420 ggcattgtcg gggtcgatac ttttcagaat gtcggccaga gcatgagcga agaagaagtc 480 gaggcctgga tggggccatt gcgtgcggat ttccgccatg gtgccagtca gttcgtgcca 540 ggcatgttca tcgaggacac tgatgccgaa ttgcgtgact gggtcgtggc agacatgtcg 600 gccgcgccgc ctgaagtggc gctcagtgcc atgcagaaca tgttggccga tatcacaagt 660 Sequence Listing (2) ggtcaggcac tctcggcgtt ctctggtctg gatgttccag tggtggccat caacgccgat 720 atctggccaa gtgatcccga ggccaatcgg aggcaccagc cccggtttga tgccgtgatc 780 atggaaaata ccgaccattt cctgcacatg gccgagcccg aggccttcaa tcgtgaattg 840 cgagcggtta tcgccaggtt gaccgagtca cgctga 876 <210> 82 <211> 291 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 82 Met Asn Ala Phe Trp Asn Arg Leu Ala Gly Ala Thr Leu Met Leu Ala Leu Met Ser Thr Ala Trp Ala Gly Val Glu Trp Pro Arg Val Ala Glu Ser Phe Asp Gly Val Pro Ile Ser Tyr Glu Ile Thr Gly Glu Gly Glu Pro Ala Leu Val Phe Ile His Gly Trp Ser Cys Asp Ala Arg Tyr Trp Arg Ala Gln Val Pro His Phe Ser Ser Asp His Gln Val Ile Ser Ile Asp Leu Ala Gly His Gly His Ser Gly Leu Glu Arg Glu Asn Tyr Thr Met Ala Ala Phe Gly Ala Asp Val Lys Ala Val Leu Asp Asp Ala Asp Ile Gln Glu Ala Ile Leu Ile Gly His Ser Met Gly Gly Pro Val Ser Val Ala Ala Ala Lys Leu Met Pro Glu Arg Val Ile Gly Ile Val Gly Sequence Listing (2) Val Asp Thr Phe Gln Asn Val. Gly Gln Ser Met Ser Glu Glu Glu Val Glu Ala Trp Met Gly Pro Leu Arg Ala Asp Phe Arg His Gly Ala Ser Gln Phe Val Pro Gly Met Phe Ile Glu Asp Thr Asp Ala Glu Leu Arg Asp Trp Val Val Ala Asp Met. Ser Ala Ala Pro Pro Glu Val Ala Leu Ser Ala Met Gin Asn Met Leu Ala Asp Ile Thr Ser Gly Gln Ala Leu Ser Ala Phe Ser Gly Leu Asp Val Pro Val Val Ala Ile Asn Ala Asp Ile Trp Pro Ser Asp Pro Glu Ala Asn Arg Arg His Gln Pro Arg Phe Asp Ala Val Ile Met Glu Asri Thr Asp His Phe Leu His Met Ala Glu Pro Glu Ala Phe Asn Arg Glu Leu Arg Ala Val Ile Ala Arg Leu Thr Glu Ser Arg <210> 83 <211> 876 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 83 atgaatgcat tctggaaccg attggcaggc gcaacgctga tgctcgcact gatgtccacg 60 gcctgggcag gtgtggaatg gccgcgagtg gccgaatcct ttgatggtgt gccgatatct 120 Sequence Listing (2) tatgaaatta ctggggaggg cgagcccgct cttgtattca tccatggctg gagttgcgat 180 gcccgctact ggcgagccca ggtgccgcac ttctcaagcg atcaccaggt gatcagtatc 240 gatctggccg gccatggaca ctccgggctg gaacgcgaga actacaccat ggccgccttc 300 ggcgcggacg tcaaggccgt gctcgatgat gccgacatcc aggaagcgat tctgattgga 360 cactccatgg gcgggccggt cagcgtggct gcggccaaat tgatgcccga acgggtgatc 420 ggcattgtcg gggtcgatac ttttcagaat gtcggccaga gcatgagcga agaagaagtc 480 gaggcctgga tggggccatt gcgtgcggat ttccgccatg gtgccagtca gttcgtgcca 540 ggcatgttca tcgaggacac tgatgccgaa ttgcgtgact gggtcgtggc agacatgtcg 600 gccgcgccgc ctgaagtggc gctcagtgcc atgcagaaca tgttggccga tatcacaagt 660 ggtcaggcac tctcggcgtt ctctggtctg gatgttccag tggtggccat caacgccgat 720 atctggccaa gtgatcccga ggccaatcgg aggcaccagc cccggtttga tgccgtgatc 780 atggaaaata ccgaccattt cctgcacatg gccgagcccg aggccttcaa tcgtgaattg 840 cgagcggtta tcgccaggtt gaccgagtca cgctga 876 <210> 84 <211> 291 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 84 Met Asn Ala Phe Trp Asn Arg Leu Ala Gly Ala Thr Leu Met Leu Ala Leu Met Ser Thr Ala Trp Ala Gly Val Glu Trp Pro Arg Val Ala Glu Ser Phe Asp Gly Val Pro Ile Ser Tyr Glu Ile Thr Gly Glu Gly Glu Pro Ala Leu Val Phe Ile His Gly Trp Ser Cys Asp Ala Arg Tyr Trp Arg Ala Gin Val Pro His Phe Ser Ser Asp His Gin Val Ile Ser Ile Sequence Listing (2) Asp Leu Ala Gly His Gly His Ser Gly Leu Glu Arg Glu Asn Tyr Thr Met Ala Ala Phe Gly Ala Asp Val Lys Ala Val Leu Asp Asp Ala Asp Ile Gln Glu Ala Ile Leu Ile Gly His Ser Met Gly Gly Pro Val Ser Val Ala Ala Ala Lys Leu Met Pro Glu Arg Val Ile Gly Ile Val Gly Val Asp Thr Phe Gln Asn Val Gly Gln Ser Met Ser Glu Glu Glu Val Glu Ala Trp Met Gly Pro Leu Arg Ala Asp Phe Arg His Gly Ala Ser Gln Phe Val Pro Gly Met Phe Ile Glu Asp Thr Asp Ala Glu Leu Arg Asp Trp Val Val Ala Asp Met. Ser Ala Ala Pro Pro Glu Val Ala Leu Ser Ala Met Gln Asn Met Leu Ala Asp Ile Thr Ser Gly Gln Ala Leu Ser Ala Phe Ser Gly Leu Asp Val Pro Val Val Ala Ile Asn Ala Asp Ile Trp Pro Ser Asp Pro Glu Ala Asn Arg Arg His Gln Pro Arg Phe Asp Ala Val Ile Met Glu Asn Thr Asp His Phe Leu His Met Ala Glu Pro Glu Ala Phe Asn Arg Glu Leu Arg Ala Val Ile Ala Arg Leu Thr Sequence Listing (2) Glu Ser Arg <210> 85 <211> 1056 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 85 gtggtgattt accgccaact gctggccggc atcctgtcgc tcagcctctc gttggccgca 60 ctggccgatg aaaacggcct gcgctttgcc gagctgggag actgtgcgct ggagagcggc 120 gagacgctcc gtgactgcaa agtgggctat cgcctgctgg gccggctgaa cgaagacagg 180 tccaatgtca tcgtctttcc gacctggtac accggaagca gcgagcacat cgtcaatttc 240 ggctatgtcg ggcccggcca catggcggat accgaccgct atttcttcgt gatcatggac 300 gccttcgcca atggcgtttc gtcgtcgccg tccaacagcc cgcaacaggc gggggacaaa 360 tttcccacgg tcacgctggg ggacatggtc cgggctcagc accgggtgct gaccgaggag 420 ctgggtttcg aacacgtgtt cgccgtcatg ggcgtgtcca tgggtggcat gcaggccttc 480 gagtgggccg tgcgctttcc gggcttcatg gacaatatcg tgtccgtgga gggcacgccc 540 tggagcacgg actatgaccg actgttgtgg ggcgcctggg cggaagcggg cgcggtccat 600 gacggcagcg aggaatccct ggcccgcgcc tcgcggctgc tggcccagct ggacgccctg 660 accctgtgga cgcccgggtt cttcaacagc atgctcaggg accaggacct ggatgagttc 720 atggaggctt tcacgcccat gctcagcgtc gggggactgg ccgaccgcaa tgcccagacc 780 gaagccgccc tggcgcatga catccggcgt ggcatcgaag gattcgaagc gcgggccgcc 840 gaaatcatcc aggcccgcgt gctgtccgtt acctatgaca ccgaccacat ggtcaacccg 900 gcgccctcgg cgcagttgat gcgctggatt ggcggggatt cggtggtgat cgccaccggc 960 tgcggacaca tgggcaccac cacggaatgc cagcaggctg agaccgctgc cgaagtgaac 1020 gccttcctgg ccggcagccc gtcggagccc aggtaa 1056 <210> 86 <211> 351 <212> PRT

Sequence Listing (2) <213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 86 Met Val Ile Tyr Arg Gln Leu Leu Ala Gly Ile Leu Ser Leu Ser Leu Ser Leu Ala Ala Leu Ala Asp Glu Asn Gly Leu Arg Phe Ala Glu Leu Gly Asp Cys Ala Leu Glu Ser Gly Glu Thr Leu Arg Asp Cys Lys Val Gly Tyr Arg Leu Leu Gly Arg Leu Asn Glu Asp Arg Ser Asn Val Ile Val Phe Pro Thr Trp Tyr Thr Gly Ser Ser Glu His Ile Val Asn Phe Gly Tyr Val Gly Pro Gly His Met Ala Asp Thr Asp Arg Tyr Phe Phe Val Ile Met Asp Ala Phe Ala Asn Gly Val Ser Ser Ser Pro Ser Asn Ser Pro Gln Gln Ala Gly Asp Lys Phe Pro Thr Val Thr Leu Gly Asp Met Val Arg Ala Gin His Arg Val Leu Thr Glu Glu Leu Gly Phe Glu His Val Phe Ala Val Met Gly Val Ser Met Gly Gly Met Gln Ala Phe Glu Trp Ala Val Arg Phe Pro Gly Phe Met Asp Asn Ile Val Ser Val Glu Gly Thr Pro Trp Ser Thr Asp Tyr Asp Arg Leu Leu Trp Gly Ala Sequence Listing (2) Trp Ala Glu Ala Gly Ala Val His Asp Gly Ser Glu Glu Ser Leu Ala Arg Ala Ser Arg Leu Leu Ala Gin Leu Asp Ala Leu Thr Leu Trp Thr Pro Gly Phe Phe Asn Ser Met Leu Arg Asp Gln Asp Leu Asp Glu Phe Met Glu Ala Phe Thr Pro Met Leu Ser Val Gly Gly Leu Ala Asp Arg Asn Ala Gln Thr Glu Ala Ala Leu Ala His Asp Ile Arg Arg Gly Ile Glu Gly Phe Glu Ala Arg Ala Ala Glu Ile Ile Gln Ala Arg Val Leu Ser Val Thr Tyr Asp Thr Asp His Met Val Asn Pro Ala Pro Ser Ala Gln Leu Met Arg Trp Ile Gly Gly Asp Ser Val Val Ile Ala Thr Gly Cys Gly His Met Gly Thr Thr Thr Glu Cys Gln Gln Ala Glu Thr Ala Ala Glu Val Asn Ala Phe Leu Ala Gly Ser Pro Ser Glu Pro Arg <210> 87 <211> 1056 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 87 gtggtgattt accgccaact gctggccggc atcctgtcgc tcagcctctc gttggccgca 60 ctggccgatg aaaacggcct gcgctttgcc gagctgggag actgtgcgct ggagagcggc 120 Sequence Listing (2) gagacgctcc gtgactgcaa agtgggctat cgcctgctgg gccggctgaa cgaagacagg 180 tccaatgtca tcgtctttcc gacctggtac accggaagca gcgagcacat cgtcaatttc 240 ggctatgtcg ggcccggcca catggcggat accgaccgct atttcttcgt gatcatggac 300 gccttcgcca atggcgtttc gtcgtcgccg tccaacagcc cgcaacaggc gggggacaaa 360 tttcccacgg tcacgctggg ggacatggtc cgggctcagc accgggtgct gaccgaggag 420 ctgggtttcg aacacgtgtt cgccgtcatg ggcgtgtcca tgggtggcat gcaggccttc 480 gagtgggccg tgcgctttcc gggcttcatg gacaatatcg tgtccgtgga gggcacgccc 540 tggagcacgg actatgaccg actgttgtgg ggcgcctggg cggaagcggg cgcggtccat 600 gacggcagcg aggaatccct ggcccgcgcc tcgcggctgc tggcccagct ggacgccctg 660 accctgtgga cgcccgggtt cttcaacagc atgctcaggg accaggacct ggatgagttc 720 atggaggctt tcacgcccat gctcagcgtc gggggactgg ccgaccgcaa tgcccagacc 780 gaagccgccc tggcgcatga catccggcgt ggcatcgaag gattcgaagc gcgggccgcc 840 gaaatcatcc aggcccgcgt gctgtccgtt acctatgaca ccgaccacat ggtcaacccg 900 gcgccctcgg cgcagttgat gcgctggatt ggcggggatt cggtggtgat cgccaccggc 960 tgcggacaca tgggcaccac cacggaatgc cagcaggctg agaccgctgc cgaagtgaac 1020 gccttcctgg ccggcagccc gtcggagccc aggtaa 1056 <210> 88 <211> 351 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 88 Met Val Ile Tyr Arg Gln Leu Leu Ala Gly Ile Leu Ser Leu Ser Leu Ser Leu Ala Ala Leu Ala Asp Glu Asn Gly Leu Arg Phe Ala Glu Leu Gly Asp Cys Ala Leu Glu Ser Gly Glu Thr Leu Arg Asp Cys Lys Val Sequence Listing (2) Gly Tyr Arg Leu Leu Gly Arg Leu Asn Glu Asp Arg Ser Asn Val Ile Val Phe Pro Thr Trp Tyr Thr Gly Ser Ser Glu His Ile Val Asn Phe Gly Tyr Val Gly Pro Gly His Met Ala Asp Thr Asp Arg Tyr Phe Phe Val Ile Met Asp Ala Phe Ala Asn Gly Val Ser Ser Ser Pro Ser Asn Ser Pro Gln Gln Ala Gly Asp Lys Phe Pro Thr Val Thr Leu Gly Asp Met Val Arg Ala Gln His Arg Val Leu Thr Glu Glu Leu Gly Phe Glu His Val Phe Ala Val Met Gly Val Ser Met Gly Gly Met Gln Ala Phe Glu Trp Ala Val Arg Phe Pro Gly Phe Met Asp Asn Ile Val Ser Val Glu Gly Thr Pro Trp Ser Thr Asp Tyr Asp Arg Leu Leu Trp Gly Ala Trp Ala Glu Ala Gly Ala Val. His Asp Gly Ser Glu Glu Ser Leu Ala Arg Ala Ser Arg Leu Leu Ala Gln Leu Asp Ala Leu Thr Leu Trp Thr Pro Gly Phe Phe Asn Ser Met Leu Arg Asp Gln Asp Leu Asp Glu Phe Met Glu Ala Phe Thr Pro Met. Leu Ser Val Gly Gly Leu Ala Asp Arg Asn Ala Gln Thr Glu Ala Ala Leu Ala His Asp Ile Arg Arg Gly Ile Sequence Listing (2) Glu Gly Phe Glu Ala Arg Ala Ala Glu Ile Ile Gln Ala Arg Val Leu Ser Val Thr Tyr Asp Thr Asp His Met Val Asn Pro Ala Pro Ser Ala Gln Leu Met Arg Trp Ile Gly Gly Asp Ser Val Val Ile Ala Thr Gly Cys Gly His Met Gly Thr Thr Thr Glu Cys Gln Gln Ala Glu Thr Ala Ala Glu Val Asn Ala Phe Leu Ala Gly Ser Pro Ser Glu Pro Arg <210> 89 <211> 1056 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 89 gtggtgattt accgccaact gctggccggc atcctgtcgc tcagcctctc gttggccgca 60 ctggccgatg aaaacggcct gcgctttgcc gagctgggag actgtgcgct ggagagcggc 120 gagacgctcc gtgactgcaa agtgggctat cgcctgctgg gccggctgaa cgaagacagg 180 tccaatgtca tcgtctttcc gacctggtac accggaagca gcgagcacat cgtcaatttc 240 ggctatgtcg ggcccggcca catggcggat accgaccgct atttcttcgt gatcatggac 300 gccttcgcca atggcgtttc gtcgtcgccg tccaacagcc cgcaacaggc gggggacaaa 360 tttcccacgg tcacgctggg ggacatggtc cgggctcagc accgggtgct gaccgaggag 420 ctgggtttcg aacacgtgtt cgccgtcatg ggcgtgtcca tgggtggcat gcaggccttc 480 gagtgggccg tgcgctttcc gggcttcatg gacaatatcg tgtccgtgga gggcacgccc 540 tggagcacgg actatgaccg actgttgtgg ggcgcctggg cggaagcggg cgcggtccat 600 gacggcagcg aggaatccct ggcccgcgcc tcgcggctgc tggcccagct ggacgccctg 660 Sequence Listing (2) accctgtgga cgcccgggtt cttcaacagc atgctcaggg accaggacct ggatgagttc 720 atggaggctt tcacgcccat gctcagcgtc gggggactgg ccgaccgcaa tgcccagacc 780 gaagccgccc tggcgcatga catccggcgt ggcatcgaag gattcgaagc gcgggccgcc 840 gaaatcatcc aggcccgcgt gctgtccgtt acctatgaca ccgaccacat ggtcaacccg 900 gcgccctcgg cgcagttgat gcgctggatt ggcggggatt cggtggtgat cgccaccggc 960 tgcggacaca tgggcaccac cacggaatgc cagcaggctg agaccgctgc cgaagtgaac 1020 gccttcctgg ccggcagccc gtcggagccc aggtaa 1056 <210> 90 <211> 351 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 90 Met Val Ile Tyr Arg Gln Leu Leu Ala Gly Ile Leu Ser Leu Ser Leu Ser Leu Ala Ala Leu Ala Asp Glu Asn Gly Leu Arg Phe Ala Glu Leu Gly Asp Cys Ala Leu Glu Ser Gly Glu Thr Leu Arg Asp Cys Lys Val Gly Tyr Arg Leu Leu Gly Arg Leu Asn Glu Asp Arg Ser Asn Val Ile Val Phe Pro Thr Trp Tyr Thr Gly Ser Ser Glu His Ile Val Asn Phe Gly Tyr Val Gly Pro Gly His Met Ala Asp Thr Asp Arg Tyr Phe Phe Val Ile Met Asp Ala Phe Ala Asn Gly Val Ser Ser Ser Pro Ser Asn Ser Pro Gln Gln Ala Gly Asp Lys Phe Pro Thr Val Thr Leu Gly Asp Sequence Listing (2) Met Val Arg Ala Gln His Arg Val Leu Thr Glu Glu Leu Gly Phe Glu His Val Phe Ala Val Met Gly Val Ser Met Gly Gly Met Gln Ala Phe Glu Trp Ala Val Arg Phe Pro Gly Phe Met Asp Asn Ile Val Ser Val Glu Gly Thr Pro Trp Ser Thr Asp Tyr Asp Arg Leu Leu Trp Gly Ala Trp Ala Glu Ala Gly Ala Val His Asp Gly Ser Glu Glu Ser Leu Ala Arg Ala Ser Arg Leu Leu Ala Gln Leu Asp Ala Leu Thr Leu Trp Thr Pro Gly Phe Phe Asn Ser Met Leu Arg Asp Gln Asp Leu Asp Glu Phe Met Glu Ala Phe Thr Pro Met Leu Ser Val Gly Gly Leu Ala Asp Arg Asn Ala Gln Thr Glu Ala Ala Leu Ala His Asp Ile Arg Arg Gly Ile Glu Gly Phe Glu Ala Arg Ala Ala Glu Ile Ile Gln Ala Arg Val Leu Ser Val Thr Tyr Asp Thr Asp His Met Val Asn Pro Ala Pro Ser Ala Gln Leu Met Arg Trp Ile Gly Gly Asp Ser Val Val Ile Ala Thr Gly Cys Gly His Met Gly Thr Thr. Thr Glu Cys Gln Gln Ala Glu Thr Ala Sequence Listing (2) Ala Glu Val Asn Ala Phe Leu Ala Gly Ser Pro Ser Glu Pro Arg <210> 91 <211> 1032 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 91 atggctacta ctggagaagc gatatcttct gcatttccgt acgagaagca gcgccggcgg 60 gttctgggga gagagatggc ctatgtggaa gtgggggccg gcgacccgat cgtgctgctg 120 cacggcaatc cgacctcatc ctacctctgg cgcaatgtcc tgccgcatct ccaactacga 180 ggccgatgca tcgcgcccga cctgattggc atgggcgact ccgataagct acctgacagc 240 ggcccgagct cgtatcgctt cgtagatcag cgccgctacc tcgatgcgct gctggaggca 300 ttggacgtac gtgagcgtgt gacgctcgtc attcatgact ggggctcggg acttggcttt 360 gactgggcca accgacaccg cgacgccgta aagggcatcg catacatgga ggcgatcgtg 420 cgcccgcagg gatgggacca ctgggacgta atgaatatgc gtccattcct agaggcgctg 480 cgttccgagg ccggcgagaa gatggtcctt gaagacaact ttttcatcga gaagatttta 540 ccaggcgctg ttctccgcaa gctcaccgcg gatgaaatgg cggagtatcg tcggccgttc 600 gctgaacccg gcgaggcgcg acgaccgact ctgacttggc cacgggagat tcctatcgat 660 ggcaaacccg ccgacgtgaa tacgattgtg gcggcctatt cggagtggct tgcgacgagc 720 gatgtgccca agctattcat aaaagccgag cccggcgcac tccttggcag cgggattaac 780 cttgaaaccg ctcgctcctg gcctgcgcag acggaagtaa ccgtggccgg agttcatttt 840 gtgcaagagg attcgccaga tgagattggg cgctcggatt ctggcgaccc ttggcccgct 900 ggcggacgaa atcgccgtct actcgccccg tctggcgcag catctcgatc actacagtcc 960 gttcgcgctc agcttcgcac tgccctgcaa tacccccggc ctgcggttcc tgtgccgcga 1020 cagcttcgat ga 1032 <210> 92 <211> 343 <212> PRT

Sequence Listing (2) <213> Unknown <220>
<223> Obtained from an environmental sample.
<400> 92 Met Ala Thr Thr Gly Glu Ala Ile Ser Ser Ala Phe Pro Tyr Glu Lys Gln Arg Arg Arg Val Leu Gly Arg Glu Met Ala Tyr Val Glu Val Gly Ala Gly Asp Pro Ile Val Leu Leu His Gly Asn Pro Thr Ser Ser Tyr Leu Trp Arg Asn Val Leu Pro His Leu Gln Leu Arg Gly Arg Cys Ile Ala Pro Asp Leu Ile Gly Met Gly Asp Ser Asp Lys Leu Pro Asp Ser Gly Pro Ser Ser Tyr Arg Phe Val Asp Gln Arg Arg Tyr Leu Asp Ala Leu Leu Glu Ala Leu Asp Val. Arg Glu Arg Val Thr Leu Val Ile His Asp Trp Gly Ser Gly Leu Gly Phe Asp Trp Ala Asn Arg His Arg Asp Ala Val Lys Gly Ile Ala Tyr Met Glu Ala Ile Val Arg Pro Gln Gly Trp Asp His Trp Asp Val Met Asn Met Arg Pro Phe Leu Glu Ala Leu Arg Ser Glu Ala Gly Glu Lys Met Val Leu Glu Asp Asn Phe Phe Ile Glu Lys Ile Leu Pro Gly Ala Val Leu Arg Lys Leu Thr Ala Asp Glu Sequence Listing (2) Met Ala Glu Tyr Arg Arg Pro Phe Ala Glu Pro Gly Glu Ala Arg Arg Pro Thr Leu Thr Trp Pro Arg Glu Ile Pro Ile Asp Gly Lys Pro Ala Asp Val Asn Thr Ile Val Ala Ala Tyr Ser Glu Trp Leu Ala Thr Ser Asp Val Pro Lys Leu Phe Ile Lys Ala Glu Pro Gly Ala Leu Leu Gly Ser Gly Ile Asn Leu Glu Thr Ala Arg Ser Trp Pro Ala Gln Thr Glu Val Thr Val Ala Gly Val His Phe Val Gln Glu Asp Ser Pro Asp Glu Ile Gly Arg Ser Asp Ser Gly Asp Pro Trp Pro Ala Gly Gly Arg Asn Arg Arg Leu Leu Ala Pro Ser Gly Ala Ala Ser Arg Ser Leu Gln Ser Val Arg Ala Gln Leu Arg Thr Ala Leu Gln Tyr Pro Arg Pro Ala Val Pro Val Pro Arg Gln Leu Arg <210> 93 <211> 1209 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 93 atgatgaaac ctctgattgc tgttctggcg ctgctgtggg catgtcagga tccgggcttc 60 actcaggctt ccacggcaac gccgccccaa gaaccagcca aggccactgc tgcggcgaag 120 Sequence Listing (2) aacgactaca gcagcggcga cgcatggctc tgccgccccg gccgccatga cacctgtgct 180 gttgatctga ccacgaccgt catctcagcc agcggcaaga tgacgagtga atcctggagc 240 gccgatcccg ccgcgccgat cgattgcttt tacgtctatc ccacggtatc gaccgatcgc 300 acgggcaaca gcgacatggt tccggggccg gaagagaagg ccgtcgtacg gacgcagttc 360 gcacgttttg cagccaaatg ccgcgcctat gcgccgctgt accgccagat cactctcgcc 420 gccttgcggg cagcgattgc cggcacaccc atcccggtag accgagccct ggcttacaat 480 gatgtgctcg acgcctggaa ctattacctg gagcatgaca acaagggtcg aggcgtggtg 540 ctgatcgggc actcgcaggg ctccgcagtg ctgacgcaac tcatcaggaa tgaaattgac 600 ggaaagccgg tgcagtcgcg catcgtctcg gccttgctgc tgggaacgaa cttgcctgta 660 ccgaagggca aggatgtagg cggcgcattt cagcacatgc ccctctgccg cgcacccgac 720 cagaccggtt gcgtgattac ctatgcctca tttcgcgcaa ccgctcctcc tccggcgaac 780 agccgcttcg gccgggtcat gggtgagaat atggtttccg ggtgcacgaa cccggcagcg 840 cttgcaggcg gaagcggcga gctgcatgct tacctgcgcg cgcaaagcag caggattgtc 900 gatgcagagg ctgagcctgg accttgggtc aagtccggaa cggcggttac caccccgttt 960 gtaagcgtgc cgggactgtt gaccgccgag tgcgtggcga acgagagcgg ctcatacctg 1020 gccgtcagct tgcacggcga tcccgcaggt ccccgcgcga atgacatcgc gggtgacgtc 1080 gtggtcaacg ggcaggttct acgtgattgg gggctgcatc tgatcgacgt gaatctggcg 1140 atgggaaatc tgctggacat cgtaggaagg cagtccaagg cgtacctgtc cgccatcgcg 1200 acgaaataa 1209 <210> 94 <211> 402 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 94 Met Met Lys Pro Leu Ile Ala Val Leu Ala Leu Leu Trp Ala Cys Gln Asp Pro Gly Phe Thr Gln Ala Ser Thr Ala Thr Pro Pro Gln Glu Pro Sequence Listing (2) Ala Lys Ala Thr Ala Ala Ala Lys Asn Asp Tyr Ser Ser Gly Asp Ala Trp Leu Cys Arg Pro Gly Arg His Asp Thr Cys Ala Val Asp Leu Thr Thr Thr Val Ile Ser Ala Ser Gly Lys Met Thr Ser Glu Ser Trp Ser Ala Asp Pro Ala Ala Pro Ile Asp Cys Phe Tyr Val Tyr Pro Thr Val Ser Thr Asp Arg Thr Gly Asn Ser Asp Met Val Pro Gly Pro Glu Glu Lys Ala Val Val Arg Thr Gln Phe Ala Arg Phe Ala Ala Lys Cys Arg Ala Tyr Ala Pro Leu Tyr Arg Gln Ile Thr Leu Ala Ala Leu Arg Ala Ala Ile Ala Gly Thr Pro Ile Pro Val Asp Arg Ala Leu Ala Tyr Asn Asp Val Leu Asp Ala Trp Asn. Tyr Tyr Leu Glu His Asp Asn Lys Gly Arg Gly Val Val Leu Ile Gly His Ser Gln Gly Ser Ala Val Leu Thr Gln Leu Ile Arg Asn Glu Ile Asp Gly Lys Pro Val Gln Ser Arg Ile Val Ser Ala Leu Leu Leu Gly Thr Asn Leu Pro Val Pro Lys Gly Lys Asp Val Gly Gly Ala Phe Gln His Met Pro Leu Cys Arg Ala Pro Asp Sequence Listing (2) Gln Thr Gly Cys Val Ile Thr Tyr Ala Ser Phe Arg Ala Thr Ala Pro Pro Pro Ala Asn Ser Arg Phe Gly Arg Val Met Gly Glu Asn Met Val Ser Gly Cys Thr Asn Pro Ala Ala Leu Ala Gly Gly Ser Gly Glu Leu His Ala Tyr Leu Arg Ala Gln Ser Ser Arg Ile Val Asp Ala Glu Ala Glu Pro Gly Pro Trp Val Lys Ser Gly Thr Ala Val Thr Thr Pro Phe Val Ser Val Pro Gly Leu Leu Thr Ala Glu Cys Val Ala Asn Glu Ser Gly Ser Tyr Leu Ala Val Ser Leu His Gly Asp Pro Ala Gly Pro Arg Ala Asn Asp Ile Ala Gly Asp Val Val Val Asn Gly Gln Val Leu Arg Asp Trp Gly Leu His Leu Ile Asp Val Asn Leu Ala Met Gly Asn Leu Leu Asp Ile Val Gly Arg Gln Ser Lys Ala Tyr Leu Ser Ala Ile Ala Thr Lys <210> 95 <211> 912 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 95 Sequence Listing (2) atgaatacgt ctctttgcgc cgccgcgctg gcggcatggg tgctgatgcc gactgcgatg 60 gccagggccg actacgaggt agtcacgcgc aacgacattg cctttgccga gcacgacggt 120 gtgaagctgc tgggggattt ctacgcgccc aaaggcctcg acaaggcgcc ggtgctggtc 180 gcagtgcacg gcggtggatg gcaggtcggc aaccgcaaat tctattcgaa ctgggccccc 240 tatctcgcca ggaacggcta cgccgtgttt gccattgaat accggctgat gaagcctggt 300 gtgaaaacct atccgggcgc gatctatgac accaaggcgg cggtgcaata tgtgcgcgcc 360 aatgcgcgcg agctcggcat cgaccccgag cgcgtcggga tggtcggtga cagcgccggc 420 gctcatcttt cggcgctggt tgctctcgcc ggcgaagatc ctgcgttctc cggcgaatat 480 cgttccgatc cgaatgcgtc ggtatcggca aagatcaagg tggtggtcgg attctacggc 540 gtgtacgaca tgctggcgca atgggagcac gacctgctgg cgcggccgcg tgacaatatc 600 tcggaaaaat ttcttggcgc ggcgccgcac acgaaccgca aagtcttttt cgaggcttcg 660 ccgatcagct acgcgacggt ggacaagaac ccaacacggt tcttgctcat ccacggcgac 720 cacgacgaca tcgtcgatcc ggcgacacag tctacggcgt ttctcgtcgc gctcaaacag 780 gcggggttct tcgtccgcac cgtggtgatc ccgggcgcgg ggcatttctt tgtcactgat 840 ccggttgacg ataccagctt cggcggtttc gccgggccga aggtcgttcg tttccttaac 900 gacacgctat aa 912 <210> 96 <211> 303 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 96 Met Asn Thr Ser Leu Cys Ala Ala Ala Leu Ala Ala Trp Val Leu Met Pro Thr Ala Met Ala Arg Ala Asp Tyr Glu Val Val Thr Arg Asn Asp Ile Ala Phe Ala Glu His Asp Gly Val Lys Leu Leu Gly Asp Phe Tyr Sequence Listing (2) Ala Pro Lys Gly Leu Asp Lys Ala Pro Val Leu Val Ala Val His Gly Gly Gly Trp Gln Val Gly Asn Arg Lys Phe Tyr Ser Asn Trp Ala Pro Tyr Leu Ala Arg Asn Gly Tyr Ala Val Phe Ala Ile Glu Tyr Arg Leu Met Lys Pro Gly Val Lys Thr Tyr Pro Gly Ala Ile Tyr Asp Thr Lys Ala Ala Val Gln Tyr Val Arg Ala Asn Ala Arg Glu Leu Gly Ile Asp Pro Glu Arg Val Gly Met Val. Gly Asp Ser Ala Gly Ala His Leu Ser Ala Leu Val Ala Leu Ala Gly Glu Asp Pro Ala Phe Ser Gly Glu Tyr Arg Ser Asp Pro Asn Ala Ser Val Ser Ala Lys Ile Lys Val Val Val Gly Phe Tyr Gly Val Tyr Asp Met Leu Ala Gln Trp Glu His Asp Leu Leu Ala Arg Pro Arg Asp Asn Ile Ser Glu Lys Phe Leu Gly Ala Ala Pro His Thr Asn Arg Lys Val. Phe Phe Glu Ala Ser Pro Ile Ser Tyr Ala Thr Val Asp Lys Asn Prc> Thr Arg Phe Leu Leu Ile His Gly Asp His Asp Asp Ile Val Asp Pro Ala Thr Gln Ser Thr Ala Phe Leu Val Ala Leu Lys Gln Ala Gly Phe Phe Val Arg Thr Val Val Ile Pro Gly Sequence Listing (2) Ala Gly His Phe Phe Val Thr Asp Pro Val Asp Asp Thr Ser Phe Gly Gly Phe Ala Gly Pro Lys Val Val Arg Phe Leu Asn Asp Thr Leu <210> 97 <211> 849 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 97 atgcgccaaa aaacaatcct gcgattggtc ggagcgatgt ttccgtttct ggccggctgc 60 tgcggaatca cgcagacagt gaatatttgg ccgggagttg cacccggttc ggaacattgg 120 aagcagacgg agcggagtgt cgaggcgtcg tacgggacgg tgatcatcga cgtggtgacg 180 ccgacgctta cggcgtatct gccggagaaa tcgagagcga ctggaaccgg tgtgattgtt 240 gcgccgggcg ggtcttgcgt tgcgctcgcg atccagcatg aaggggtaga cgtagcgaaa 300 tggctgcagg aacgtgggat cgcagccttt gtgctgaaat ataggatccc ggagaaaaaa 360 ggcgaaggca tcccggcgga tatcaatttc gatgaggcct gcagatatgg catcgctgac 420 ggaattcagg cgatgaaagt ggtgcggcag catgcggcgg agtggggcgt tgtgccggag 480 cgagtagggt tcatgggatt ttccgcgggg gcgatggtga cgagcggggt gttgctgcag 540 tcggatgcgg cgtcgcgtcc agcgtttgtg gccccgatct atggtggacc gtttggagtg 600 atgcccaaga ttcctgccaa tcttccgccg attttcatgg catgggccca ggatgatgac 660 cttgtgcgcg acccggtgac caggttctat gaggcattaa cagcagcggg caatcggccc 720 gagtttcaca tttattcggc gggcaagcac ggatttggca tgaggaagca gggaaccacg 780 agcgatcatt ggatcgatga gttttactgg tggcttgagg cgcaaggact gacaaaaccg 840 gcgaggtga 849 <210> 98 <211> 282 <212> PRT

Sequence Listing (2) <213> Unknown <220>
<223> Obtained from an environmental sample <400> 98 Met Arg Gln Lys Thr Ile Leu Arg Leu Val Gly Ala Met Phe Pro Phe Leu Ala Gly Cys Cys Gly Ile Thr Gln Thr Val Asn Ile Trp Pro Gly Val Ala Pro Gly Ser Glu His Trp Lys Gln Thr Glu Arg Ser Val Glu Ala Ser Tyr Gly Thr Val Ile Ile Asp Val Val Thr Pro Thr Leu Thr Ala Tyr Leu Pro Glu Lys Ser Arg Ala Thr Gly Thr Gly Val Ile Val Ala Pro Gly Gly Ser Cys Val Ala Leu Ala Ile Gln His Glu Gly Val Asp Val Ala Lys Trp Leu Gln Glu Arg Gly Ile Ala Ala Phe Val Leu Lys Tyr Arg Ile Pro Glu Lys Lys Gly Glu Gly Ile Pro Ala Asp Ile Asn Phe Asp Glu Ala Cys Arg Tyr Gly Ile Ala Asp Gly Ile Gln Ala Met Lys Val Val Arg Gln His Ala Ala Glu Trp Gly Val Val Pro Glu Arg Val Gly Phe Met Gly Phe Ser Ala Gly Ala Met Val Thr Ser Gly Val Leu Leu Gln Ser Asp Ala Ala Ser Arg Pro Ala Phe Val Ala Pro Sequence Listing (2) Ile Tyr Gly Gly Pro Phe Gly Val Met Pro Lys Ile Pro Ala Asn Leu Pro Pro Ile Phe Met Ala Trp Ala Gln Asp Asp Asp Leu Val Arg Asp Pro Val Thr Arg Phe Tyr Glu Ala Leu Thr Ala Ala Gly Asn Arg Pro Glu Phe His Ile Tyr Ser Ala Gly Lys His Gly Phe Gly Met Arg Lys Gln Gly Thr Thr Ser Asp His Trp Ile Asp Glu Phe Tyr Trp Trp Leu Glu Ala Gln Gly Leu Thr Lys Pro Ala Arg <210> 99 <211> 978 <212> DNA
<213> Thermotoga maritima MSB8 <400> 99 atggccttct tcgatttacc actcgaagaa ctgaagaaat atcgtccaga gcggtacgaa 60 gagaaagact tcgatgagtt ctgggaagag acactcgcag agagcgaaaa gttcccctta 120 gaccccgtct tcgagaggat ggagtctcac ctcaaaacag tcgaagcgta cgatgtcacc 180 ttctccggat acaggggaca gaggatcaaa gggtggctcc ttgttccaaa actggaagaa 240 gaaaaacttc cctgcgttgt gcagtacata ggatacaacg gtggaagagg attccctcac 300 gactggctgt tctggccttc tatgggttac atatgtttcg tcatggatac tcgaggtcag 360 ggaagcggct ggctgaaagg agacacaccg gattaccctg agggtcccgt tgaccctcag 420 tatccaggat tcatgacaag aggaatactg gatcccagaa cttactacta cagacgagtc 480 ttcacggacg ctgtcagagc cgttgaagct gctgcttctt ttcctcaggt agatcaagaa 540 agaatcgtga tagctggagg cagtcagggt ggcggaatag cccttgcggt gagcgctctc 600 tcaaagaaag caaaggctct tctgtgcgat gtgccgtttc tgtgtcactt cagaagagca 660 gtacagcttg tggatacgca tccatacgcg gagatcacga actttctaaa gacccacaaa 720 Sequence Listing (2) gacaaggaag aaatcgtgtt caggactctt tcctatttcg atggagtgaa cttcgcagcc 780 agagcgaaga tccctgcgct gttttctgtg ggtctcatgg acaacatttg tcctccttca 840 acggttttcg ctgcctacaa ttactacgct ggaccgaagg aaatcagaat ctatccgtac 900 aacaaccacg agggaggagg ctctttccaa gcggttgaac aggtgaaatt cttgaaaaaa 960 ctatttgaga aaggctaa 978 <210> 100 <211> 325 <212> PRT
<213> Thermotoga maritima MSB8 <400> 100 Met Ala Phe Phe Asp Leu Pro Leu Glu Glu Leu Lys Lys Tyr Arg Pro Glu Arg Tyr Glu Glu Lys Asp Phe Asp Glu Phe Trp Glu Glu Thr Leu Ala Glu Ser Glu Lys Phe Pro Leu Asp Pro Val Phe Glu Arg Met Glu Ser His Leu Lys Thr Val Glu Ala Tyr Asp Val Thr Phe Ser Gly Tyr Arg Gly Gln Arg Ile Lys Gly Trp Leu Leu Val Pro Lys Leu Glu Glu Glu Lys Leu Pro Cys Val Val. Gln Tyr Ile Gly Tyr Asn Gly Gly Arg Gly Phe Pro His Asp Trp Leu Phe Trp Pro Ser Met Gly Tyr Ile Cys Phe Val Met Asp Thr Arg Gly Gln Gly Ser Gly Trp Leu Lys Gly Asp Thr Pro Asp Tyr Pro Glu Gly Pro Val Asp Pro Gln Tyr Pro Gly Phe Sequence Listing (2) Met Thr Arg Gly Ile Leu Asp Pro Arg Thr Tyr Tyr Tyr Arg Arg Val Phe Thr Asp Ala Val Arg Ala Val Glu Ala Ala Ala Ser Phe Pro Gln Val Asp Gln Glu Arg Ile Val Ile Ala Gly Gly Ser Gln Gly Gly Gly Ile Ala Leu Ala Val Ser Ala Leu Ser Lys Lys Ala Lys Ala Leu Leu Cys Asp Val Pro Phe Leu Cys His Phe Arg Arg Ala Val Gln Leu Val Asp Thr His Pro Tyr Ala Glu Ile Thr Asn Phe Leu Lys Thr His Arg Asp Lys Glu Glu Ile Val Phe Arg Thr Leu Ser Tyr Phe Asp Gly Val Asn Phe Ala Ala Arg Ala Lys Ile Pro Ala Leu Phe Ser Val Gly Leu Met Asp Asn Ile Cys Pro Pro Ser Thr Val Phe Ala Ala Tyr Asn Tyr Tyr Ala Gly Pro Lys Glu Ile Arg Ile Tyr Pro Tyr Asn Asn His Glu Gly Gly Gly Ser Phe Gln Ala Val Glu Gln Val Lys Phe Leu Lys Lys Leu Phe Glu Lys Gly <210> 101 <211> 717 <212> DNA
<213> Unknown Sequence Listing (2) <220>
<223> Obtained from an environmental sample <400> 101 atgtactgcg tcttcgcttc ttcgaaagtc tcgaagaaca aggtggctcc gctaattgtt 60 tcgctgcatg gcctgggtgc cggtcctggc gtcatgatga ccaaaaacgc catcgacctg 120 gcggaagccg gcggctacgt agtcgtcgcg ccgatgggct acaacacggg cggctggtat 180 ggatctccgg tattgcgtgg ccgcggcgga gatggaaagg gtaaggcgtc cgagagtggt 240 gctccaaacg gtacggctcc gaaagccacg gccccggcgc aaccgccaaa cctgggtgag 300 ctgagcgaga aggacgtgat gaacgtcatc gccatgatcc gtaaggagta caaaatcgat 360 gccaagcgga tgtatctaat gggtcactca atgggtgggg caggcacgtt gttcctggga 420 tcgaagcacg cgtcgatgtg ggccgcgatt gctgccgagg ctcccgccgc attttccatg 480 cagaacaacc gcgccgagta cttgcaaaag ctgaaagacg cgaaggttcc ggtgatgatc 540 gtgcacggcg atatggacga agcagttccg gtggcgaaca gccgggcgtg ggctgacacc 600 atgaaggaga tgaaaatgac gtacgaatac gtcgagcaac cgggaatcag tcacggcccg 660 gtgatcgaat ccggcatggc gccaatctac ggcttcttcg agaagcataa gaggtag 717 <210> 102 <211> 238 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 102 Met Tyr Cys Val Phe Ala Ser Ser Lys Val Ser Lys Asn Lys Val Ala Pro Leu Ile Val Ser Leu His Gly Leu Gly Ala Gly Pro Gly Val Met Met Thr Lys Asn Ala Ile Asp Leu Ala Glu Ala Gly Gly Tyr Val Val Val Ala Pro Met Gly Tyr Asn Thr Gly Gly Trp Tyr Gly Ser Pro Val Sequence Listing (2) Leu Arg Gly Arg Gly Gly Asp Gly Lys Gly Lys Ala Ser Glu Ser Gly Ala Pro Asn Gly Thr Ala Pro Lys Ala Thr Ala Pro Ala Gln Pro Pro Asn Leu Gly Glu Leu Ser Glu Lys Asp Val Met Asn Val Ile Ala Met Ile Arg Lys Glu Tyr Lys Ile Asp Ala Lys Arg Met Tyr Leu Met Gly His Ser Met Gly Gly Ala Gly Thr Leu Phe Leu Gly Ser Lys His Ala Ser Met Trp Ala Ala Ile Ala Ala Glu Ala Pro Ala Ala Phe Ser Met Gln Asn Asn Arg Ala Glu Tyr Leu Gln Lys Leu Lys Asp Ala Lys Val Pro Val Met Ile Val His Gly Asp Met Asp Glu Ala Val Pro Val Ala Asn Ser Arg Ala Trp Ala Asp Thr Met Lys Glu Met Lys Met Thr Tyr Glu Tyr Val Glu Gln Pro Gly Ile Ser His Gly Pro Val Ile Glu Ser Gly Met Ala Pro Ile Tyr Gly Phe Phe Glu Lys His Lys Arg <210> 103 <211> 870 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 103 Sequence Listing (2) atgaaaaaat tatttgcagg ctttgtctta ttcatctctt tttattcgca cgcgcaaacg 60 gtgatcaatc tttacaatgg cgctgcacct ggttccgaaa actggaactg gagcgaaacg 120 caaattaagg ttgacatagg caccatcata atggatgttg ctcatccgtc attaacagca 180 tatgtaccgg caaacccgaa tggcacttca gtgatcattg cacccggtgg tgcatttcat 240 gcactggctt ttgaccttga aggcaccgaa gtggcaaaac gcttaaacgc aaaaggcatc 300 actgcttttg tattgaaata tcgcctggtg cacaatgatc cggcacatcc tgagaacagt 360 atcggaacat tgatggcaac aaagaatttt aaaaaactgg attcgctcaa cgcgcctgtt 420 gtaccgcttg ccatgcagga tggattaacg gctgtaaaat atgtacgtca acatgcggca 480 gaatataaaa ttgatgcaga caaaattggt tttatgggtt tctctgcggg tggaacagta 540 accatgtcag ttgtatacaa tgctacagac gaaaaccgtc caaattttgt ggcgccaatt 600 tatgcatatg aaggcgcgat aattggttca acggttcctg ctgccaaaac acccatattt 660 attgcagcag caagtgacga cgatttaggc ctggcaacac acagcgtgca tatttatctg 720 aaatggcttg aagcaaagca gcctgctgaa ttacacatgt atgaacaagg caaacatggt 780 tttggtacaa aaaagcaagg tcttcctgtt gatgcatgga tggatcggtt tactgattgg 840 cttgccatgc atgggttgat aaaacagtaa 870 <210> 104 <211> 289 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 104 Met Lys Lys Leu Phe Ala Gly Phe Val Leu Phe Ile Ser Phe Tyr Ser His Ala Gln Thr Val Ile Asn Leu Tyr Asn Gly Ala Ala Pro Gly Ser Glu Asn Trp Asn Trp Ser Glu Thr Gln Ile Lys Val Asp Ile Gly Thr Ile Ile Met Asp Val Ala His Pro Ser Leu Thr Ala Tyr Val Pro Ala Sequence Listing (2) Asn Pro Asn Gly Thr Ser Val Ile Ile Ala Pro Gly Gly Ala Phe His Ala Leu Ala Phe Asp Leu Glu Gly Thr Glu Val Ala Lys Arg Leu Asn Ala Lys Gly Ile Thr Ala Phe Val Leu Lys Tyr Arg Leu Val His Asn Asp Pro Ala His Pro Glu Asn Ser Ile Gly Thr Leu Met Ala Thr Lys Asn Phe Lys Lys Leu Asp Ser Leu Asn Ala Pro Val Val Pro Leu Ala Met Gln Asp Gly Leu Thr Ala Val Lys Tyr Val Arg Gln His Ala Ala Glu Tyr Lys Ile Asp Ala Asp Lys Ile Gly Phe Met Gly Phe Ser Ala Gly Gly Thr Val Thr Met Ser Val Val Tyr Asn Ala Thr Asp Glu Asn Arg Pro Asn Phe Val Ala Pro Ile Tyr Ala Tyr Glu Gly Ala Ile Ile Gly Ser Thr Val Pro Ala Ala Lys Thr Pro Ile Phe Ile Ala Ala Ala Ser Asp Asp Asp Leu Gly Leu Ala Thr His Ser Val His Ile Tyr Leu Lys Trp Leu Glu Ala Lys Gln Pro Ala Glu Leu His Met Tyr Glu Gln Gly Lys His Gly Phe Gly Thr Lys Lys Gln Gly Leu Pro Val Asp Ala Sequence Listing (2) Trp Met Asp Arg Phe Thr Asp Trp Leu Ala Met His Gly Leu Ile Lys Gln <210> 105 <211> 969 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 105 atgagctgga tgcaatcggc cgctagcatc cttgccctgg cgctggccct actggccggc 60 ggggtgtggc tcgtggactg gcgcgccgac cggcgcgagg cggaagcgct cgcgcagtgg 120 ccgccggagg gcatttttgt ccaggttgag gaccgccgag tccacgccgt cacgcggggg 180 caaggccccg acctcgtcct gatccacggc gcctcgggca atgcgcggga tatgaccttc 240 cgctttatgg gcctgctaac ggaccgctac cgtgtcaccg cgttcgaccg tccgggcctg 300 ggctacaccg accccgcgcc ggccgattgg ggcgagagcc cgaaggagca ggccgccttc 360 ctacaggccg cagcgcggga gctgcatatc gagagcccca tcgtcctggg ccactccttc 420 ggcggggctg tggcgctggc ctggggattg agcgacccgg aggggaccgc cgccgtcgtg 480 gacgtttccg gtgtggcgat gccctggccc ggccgcctgg gctggctcta ccgcatcaat 540 ggcccgtggc tcggtacggc ccttgtgccg cctctcgttg ctgcctttgc gccccaaccg 600 ctcctagacc gggcgctcgc ggcgacgttc gcgcccgatc ccgtccccga aggctatgcc 660 gagcacttcg gagccagcct ctcgatccga cgcgcctcgt ttcggaccaa cgcccgtcag 720 gtgaacgcgc tgcgggcctc ggtcatggag atgtcccggg actattcaac cttctccgtg 780 ccccttgaaa tcgtccacgg cgaagccgac accattgtcc ccctcgaggt ccattcctgc 840 cctctgtcgc gaatcattcc tggcgcgaac cttacggtgc tgcccggcgt cggccatatg 900 ccgcaccaga cgcaccccga ggcggtggtc gccgctgtcg atcgggcgcg agcccgcgct 960 gggctctga 969 <210> 106 Sequence Listing (2) <211> 322 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 106 Met Ser Trp Met Gln Ser Ala Ala Ser Ile Leu Ala Leu Ala Leu Ala Leu Leu Ala Gly Gly Val Trp Leu Val Asp Trp Arg Ala Asp Arg Arg Glu Ala Glu Ala Leu Ala Gln Trp Pro Pro Glu Gly Ile Phe Val Gln Val Glu Asp Arg Arg Val His Ala Val Thr Arg Gly Gln Gly Pro Asp Leu Val Leu Ile His Gly Ala. Ser Gly Asn Ala Arg Asp Met Thr Phe Arg Phe Met Gly Leu Leu Thr Asp Arg Tyr Arg Val Thr Ala Phe Asp Arg Pro Gly Leu Gly Tyr Thr Asp Pro Ala Pro Ala Asp Trp Gly Glu Ser Pro Lys Glu Gln Ala Ala. Phe Leu Gln Ala Ala Ala Arg Glu Leu His Ile Glu Ser Pro Ile Val Leu Gly His Ser Phe Gly Gly Ala Val Ala Leu Ala Trp Gly Leu Ser Asp Pro Glu Gly Thr Ala Ala Val Val Asp Val Ser Gly Val Ala Met Pro Trp Pro Gly Arg Leu Gly Trp Leu Tyr Arg Ile Asn Gly Pro Trp Leu Gly Thr Ala Leu Val Pro Pro Leu Sequence Listing (2) Val Ala Ala Phe Ala Pro Gln Pro Leu Leu Asp Arg Ala Leu Ala Ala Thr Phe Ala Pro Asp Pro Val Pro Glu Gly Tyr Ala Glu His Phe Gly Ala Ser Leu Ser Ile Arg Arg Ala Ser Phe Arg Thr Asn Ala Arg Gln Val Asn Ala Leu Arg Ala Ser Val Met Glu Met Ser Arg Asp Tyr Ser Thr Phe Ser Val Pro Leu Glu Ile Val His Gly Glu Ala Asp Thr Ile Val Pro Leu Glu Val His Ser Cys Pro Leu Ser Arg Ile Ile Pro Gly Ala Asn Leu Thr Val Leu Pro Gly Val Gly His Met Pro His Gln Thr His Pro Glu Ala Val Val Ala Ala Val Asp Arg Ala Arg Ala Arg Ala Gly Leu <210> 107 <211> 1005 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 107 atgcgaaaga agctggttcg tctgttggcg tggatcgccg gcctttgcct gcttgggctg 60 gtgctgctgg ttgccgcgtt ctgggcgccg tcgcggtcgg tggacgacct caaggcgcgc 120 tgggcaacgc cgccttccac cttcctggcc atcgacggca tgcaggtgca cctgcgcgac 180 Sequence Listing (2) gaagggccgc gcgacgaccc ggcacccttg ctgctcctgc acggcacgtc cgacagcctg 240 catacctggg agggctgggt gccgccgctg gtgcaggcgg ggcaccgggt catccgcgtg 300 gacctgccgg gttttggcct gaccgggccg ttccccgacg gtgaccaccg cattgccgcg 360 tacccgatat tcctgtcaca cctgctcgac gcgctggacg tgcgccgggt ggtggtggcg 420 ggcaattcct tcggtggcca ggttgcgctg gagctggcgc tggccgagcc cgatcgcgtc 480 gagcggctgg tgctggtgga cgcgctggcc tatccacgca atgccaccaa ggtgcccgtc 540 gcgttcgtgg tggcgtcggt gccgggcttg aaccggatca tggactacgt gctgccgcgt 600 tcgctggtcg aaggcagcgt gcgcagcgta tatggcgatc cgtcgcgggt gacgccggcg 660 ctggtggacc gctactacga actggccctg cgcgagggca accgaacggc gctgcgcaac 720 cgcttcgccg ggcttcccga cgaggcctac gcgcagcgcg tgaagcggct ccgtttgccg 780 gtgctgctgc tgtggggcgg gcgcgaccag ctcattccta ttgcggcggc cgaccgcttc 840 ctggccgaca tcccgggcag ccggctggtg gcgttcccgg acctgggcca cgtgccgcag 900 gaggaggacc cgttggcttc ggtggcgccg gtgctggcgt tccttcgtga agccgacccg 960 gccgaacccg gcgccagctc agcgcaggaa gtgcagggcc agtga 1005 <210> 108 <211> 334 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 108 Met Arg Lys Lys Leu Val Arg Leu Leu Ala Trp Ile Ala Gly Leu Cys Leu Leu Gly Leu Val Leu Leu Val Ala Ala Phe Trp Ala Pro Ser Arg Ser Val Asp Asp Leu Lys Ala Arg Trp Ala Thr Pro Pro Ser Thr Phe Leu Ala Ile Asp Gly Met Gln Val His Leu Arg Asp Glu Gly Pro Arg Sequence Listing (2) Asp Asp Pro Ala Pro Leu Leu Leu Leu His Gly Thr Ser Asp Ser Leu His Thr Trp Glu Gly Trp Val Pro Pro Leu Val Gln Ala Gly His Arg Val Ile Arg Val Asp Leu Pro Gly Phe Gly Leu Thr Gly Pro Phe Pro Asp Gly Asp His Arg Ile Ala Ala Tyr Pro Ile Phe Leu Ser His Leu Leu Asp Ala Leu Asp Val Arg Arg Val Val Val Ala Gly Asn Ser Phe Gly Gly Gln Val Ala Leu Glu Leu Ala Leu Ala Glu Pro Asp Arg Val Glu Arg Leu Val Leu Val Asp Ala Leu Ala Tyr Pro Arg Asn Ala Thr Lys Val Pro Val Ala Phe Val Val Ala Ser Val Pro Gly Leu Asn Arg Ile Met Asp Tyr Val Leu Pro Arg Ser Leu Val Glu Gly Ser Val Arg Ser Val Tyr Gly Asp Pro Ser Arg Val Thr Pro Ala Leu Val Asp Arg Tyr Tyr Glu Leu Ala Leu Arg Glu Gly Asn Arg Thr Ala Leu Arg Asn Arg Phe Ala Gly Leu Pro Asp Glu Ala Tyr Ala Gln Arg Val Lys Arg Leu Arg Leu Pro Val Leu Leu Leu Trp Gly Gly Arg Asp Gln Leu Ile Pro Ile Ala Ala Ala Asp Arg Phe Leu Ala Asp Ile Pro Gly Ser Arg Sequence Listing (2) Leu Val Ala Phe Pro Asp Leu Gly His Val Pro Gln Glu Glu Asp Pro Leu Ala Ser Val Ala Pro Val Leu Ala Phe Leu Arg Glu Ala Asp Pro Ala Glu Pro Gly Ala Ser Ser Ala Gln Glu Val Gln Gly Gln <210> 109 <211> 825 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 109 atgaaaaagg aaacgatcat ccattccttt gatggaactg aactgtatat gcaaaaaaat 60 acgcctcaaa atccgcatgc ggtaatggtt attgtccatg gacttggcga gcactcgggc 120 cggtatgagt atgtaacaga aaaattgaac ggatatggct atgccgtcta ccgttttgac 180 aaccgcggcc atgggaaatc cggcggtgcg cgaggctatg tggaggatgc gtctaaattc 240 gttcatgatg cacatgaagt cgtaaaggcg gctcgtcatg actatccgaa actgccgatt 300 ttcatgctag gccacagtat gggcggattt attgcggcgt cttatggtgt tcaatacaaa 360 aatacattgg ccggccaaat tttctccggg cctgtcattc tggaactgcc ggcattcgat 420 gagttaaaaa agctggatat ggaaaaagag gcgctgacgc ggattccgaa cggactggct 480 cacctgatct gccgcagccc ggctgtcgtc caggattacg aaaatgaccc atacaattta 540 aaagaattca ccgcaaaact gctgacgact gtttttcacg agggtgtaca acatttgctt 600 caaaagctcc ctcagtacac atatccttgt tatattttac acggcgagga cgatcaaatc 660 gttcctgccg attcaggaag atacttatac gatcacattg cctctgaaga taaatctatt 720 tgtgtaatcc cacacctcta tcatgaaata ctgaatgagg aaaaagaaaa agacgagctt 780 ttacaagaaa tattcgaatg gatagaagaa cgaatacaaa aataa 825 <210> 110 Sequence Listing (2) <211> 274 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 110 Met Lys Lys Glu Thr Ile Ile His Ser Phe Asp Gly Thr Glu Leu Tyr Met Gln Lys Asn Thr Pro Gln Asn Pro His Ala Val Met Val Ile Val His Gly Leu Gly Glu His Ser Gly Arg Tyr Glu Tyr Val Thr Glu Lys Leu Asn Gly Tyr Gly Tyr Ala. Val Tyr Arg Phe Asp Asn Arg Gly His Gly Lys Ser Gly Gly Ala Arg Gly Tyr Val Glu Asp Ala Ser Lys Phe Val His Asp Ala His Glu Val. Val Lys Ala Ala Arg His Asp Tyr Pro Lys Leu Pro Ile Phe Met Leu Gly His Ser Met Gly Gly Phe Ile Ala Ala Ser Tyr Gly Val Gln Tyr Lys Asn Thr Leu Ala Gly Gln Ile Phe Ser Gly Pro Val Ile Leu Glu Leu Pro Ala Phe Asp Glu Leu Lys Lys Leu Asp Met Glu Lys Glu Ala Leu Thr Arg Ile Pro Asn Gly Leu Ala His Leu Ile Cys Arg Ser Pro Ala Val Val Gln Asp Tyr Glu Asn Asp Pro Tyr Asn Leu Lys Glu Phe Thr Ala Lys Leu Leu Thr Thr Val Phe Sequence Listing (2) His Glu Gly Val Gln His Leu Leu Gln Lys Leu Pro Gln Tyr Thr Tyr Pro Cys Tyr Ile Leu His Gly Glu Asp Asp Gln Ile Val Pro Ala Asp Ser Gly Arg Tyr Leu Tyr Asp His Ile Ala Ser Glu Asp Lys Ser Ile Cys Val Ile Pro His Leu Tyr His Glu Ile Leu Asn Glu Glu Lys Glu Lys Asp Glu Leu Leu Gln Glu Ile Phe Glu Trp Ile Glu Glu Arg Ile Gln Lys <210> 111 <211> 750 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 111 atgtcaggca agtacactgt tttggaaggc gctgaaccgt tttattttga aggaagcaaa 60 gttgggattc ttgtgtcgca tgggtttacc ggcagcaccc aaagcatgag gccgcttggg 120 gaagcctatg caaaggcagg ttatacggtt tgcggcccgc gtctgaaggg gcatggcac:c 180 cattacgagg agatggaaca gactacatat aacgattgga ttgattcagt cgaggaaggc 240 taccagtggc tgaaggaacg ctgcgataca atatttgtaa ctggtttgtc catgggcgga 300 acccttaccc tgtatatggc tgaaaatcat cctgaaatta aggcaatcat accaattaat 360 gcagctattc aaatttccga catggccgct gcagccaacc ttgatggagt gcgattcctt 420 gatgcgattg gttccgacat taagaagcag ggtgaaaaag agctcgctta cgagaaaact 480 cctgtaaagt cccttggtga gatcgtagcg ttaatggaaa aggttaaagc tgacctttcc 540 Sequence Listing (2) gatatcactt gtccagcact tatttttgtt tcaaaggaag atcacgttgt gccgccagat 600 aactcaagga ttatcaagga acaaatccgt tctgccgata agagcctgat tgagctgtca 660 gaaagctatc atgttgccac actggacaat gaccaggaga tcattatcaa ggaaacatta 720 aaattcattg aggaacagct tgggaaataa 750 <210> 112 <211> 249 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 112 Met Ser Gly Lys Tyr Thr Val. Leu Glu Gly Ala Glu Pro Phe Tyr Phe Glu Gly Ser Arg Val Gly Ile Leu Val Ser His Gly Phe Thr Gly Ser Thr Gln Ser Met Arg Pro Leu Gly Glu Ala Tyr Ala Lys Ala Gly Tyr Thr Val Cys Gly Pro Arg Leu Lys Gly His Gly Thr His Tyr Glu Glu Met Glu Gln Thr Thr Tyr Asri Asp Trp Ile Asp Ser Val Glu Glu Gly Tyr Gln Trp Leu Lys Glu Arg Cys Asp Thr Ile Phe Val Thr Gly Leu Ser Met Gly Gly Thr Leu Thr. Leu Tyr Met Ala Glu Asn His Pro Glu Ile Lys Ala Ile Ile Pro Ile Asn Ala Ala Ile Gln Ile Ser Asp Met Ala Ala Ala Ala Asn Leu Asp Gly Val Arg Phe Leu Asp Ala Ile Gly Sequence Listing (2) Ser Asp Ile Lys Lys Gln Gly Glu Lys Glu Leu Ala Tyr Glu Lys Thr Pro Val Lys Ser Leu Gly Glu Ile Val Ala Leu Met Glu Lys Val Lys Ala Asp Leu Ser Asp Ile Thr Cys Pro Ala Leu Ile Phe Val Ser Lys Glu Asp His Val Val Pro Pro Asp Asn Ser Arg Ile Ile Lys Glu Gln Ile Arg Ser Ala Asp Lys Ser Leu Ile Glu Leu Ser Glu Ser Tyr His Val Ala Thr Leu Asp Asn Asp Gln Glu Ile Ile Ile Lys Glu Thr Leu Lys Phe Ile Glu Glu Gln Leu Gly Lys <210> 113 <211> 1233 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 113 atgaaccggc tcagcctcgt cctcctctcc ctgctgctcc cggccgccgc ggccgcgcag 60 agcttcgccg tcgtcaacgc ccgcatcttc gacggcgagc gcacgatccc gcgcggcacg 120 gtcgtggtga gggacggcaa gttcgaatcg gtgggagtgg acgtgaaggt ccccgagggg 180 atggaacgga tcgacgcggg cggcggcacg ctgcttcccg ggctcatcga cgcgcacacc 240 catgccttcc tcggcggcct ggaccgggcg ctcgtcttcg gcgtcaccac cgagctcgac 300 atgggcaacg ctccggcggt ggcggcccac ctgcgcgagg agcaggcccg ccccggcggc 360 gtgcccggcc gcgccgacct gttcagcgcc ggcaccctcg cgaccgcgcc gaacgggcac 420 ggcacccagt acgggctgcc gatcccgacg ctctcccgtc ccgaagaggc tcaggactgg 480 Sequence Listing (2) gtggacgccc gcatcgccga gggctcggac tacatcaaga tcgtgatcga ggacctctcc 540 gcctacggca agtcccgccc gaccctggac cgggagaccg tcatcgcgct cgtcaaggcg 600 gcccacgccc gcggcaagct ggccgtcgtc cacgtcagca ccgcggccac cgcccggcag 660 gcgatcgaag ccggagcgga cggcctcgtg catctcttca ccgaccgcgc cgccgatccc 720 gatttcgcca agctcgtggc cgagcgcaag gccttcgtca ttccgacgct cacggtcctg 780 gagagcgcca acggcatcgc ggccggccgc accctggtcg aggacgtccg cctccgcggg 840 ctgctgctgc cgagcgaggt cgagcacctg aagacgttct acccggtgaa gaccgcgggc 900 ggcatgccgg tcgcgctcga caccgtgcgc cagctcgccg ccgccggggt gccggtcctc 960 gccggcagcg acacgccgaa cccgggcacg atgcacggcg cgagcgtcca tcgcgagatg 1020 gagctcctgg tgcaggcggg tctcacgccc gagcaggccc tggccgccgc cacctcggcg 1080 cccgcccgcg ccttccacct cgccgaccgc ggccggatcg caccgggtct ccgggccgat 1140 ctggtcctgg tggccggaga ccctctcaag gacatcaccg agactcggaa cctggtgcgg 1200 gtgtggaagg gcggagtggc ggtcaagcgg tag 1233 <210> 114 <211> 410 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 114 Met Asn Arg Leu Ser Leu Val. Leu Leu Ser Leu Leu Leu Pro Ala Ala Ala Ala Ala Gln Ser Phe Ala Val Val Asn Ala Arg Ile Phe Asp Gly Glu Arg Thr Ile Pro Arg Gly Thr Val Val Val Arg Asp Gly Lys Phe Glu Ser Val Gly Val Asp Val. Lys Val Pro Glu Gly Met Glu Arg Ile Asp Ala Gly Gly Gly Thr Leu Leu Pro Gly Leu Ile Asp Ala His Thr Sequence Listing (2) His Ala Phe Leu Gly Gly Leu Asp Arg Ala Leu Val Phe Gly Val Thr Thr Glu Leu Asp Met Gly Asn Ala Pro Ala Val Ala Ala His Leu Arg Glu Glu Gln Ala Arg Pro Gly Gly Val Pro Gly Arg Ala Asp Leu Phe Ser Ala Gly Thr Leu Ala Thr Ala Pro Asn Gly His Gly Thr Gln Tyr Gly Leu Pro Ile Pro Thr Leu Ser Arg Pro Glu Glu Ala Gln Asp Trp Val Asp Ala Arg Ile Ala Glu Gly Ser Asp Tyr Ile Lys Ile Val Ile Glu Asp Leu Ser Ala Tyr Gly Lys Ser Arg Pro Thr Leu Asp Arg Glu Thr Val Ile Ala Leu Val Lys Ala Ala His Ala Arg Gly Lys Leu Ala Val Val His Val Ser Thr Ala Ala Thr Ala Arg Gln Ala Ile Glu Ala Gly Ala Asp Gly Leu Val His Leu Phe Thr Asp Arg Ala Ala Asp Pro Asp Phe Ala Lys Leu Val Ala Glu Arg Lys Ala Phe Val Ile Pro Thr Leu Thr Val Leu Glu Ser Ala Asn Gly Ile Ala Ala Gly Arg Thr Leu Val Glu Asp Val Arg Leu Arg Gly Leu Leu Leu Pro Ser Glu Val Glu Sequence Listing (2) His Leu Lys Thr Phe Tyr Pro Val Lys Thr Ala Gly Gly Met Pro Val Ala Leu Asp Thr Val Arg Gln Leu Ala Ala Ala Gly Val Pro Val Leu Ala Gly Ser Asp Thr Pro Asn Pro Gly Thr Met His Gly Ala Ser Val His Arg Glu Met Glu Leu Leu Val Gln Ala Gly Leu Thr Pro Glu Gln Ala Leu Ala Ala Ala Thr Ser Ala Pro Ala Arg Ala Phe His Leu Ala Asp Arg Gly Arg Ile Ala Pro Gly Leu Arg Ala Asp Leu Val Leu Val Ala Gly Asp Pro Leu Lys Asp Ile Thr Glu Thr Arg Asn Leu Val Arg Val Trp Lys Gly Gly Val Ala Val Lys Arg <210> 115 <211> 1074 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 115 atgagagtag aagagatggt actgaatggg gaaagaaatg taacattaac agcttatatc 60 caggaagttg ccggagagtt tcgcaatatt gaaaagcggc cggcaatatt gatccttccg 120 gggggagggt accagttttg ctcggacaga gaggctgatc cggtggcaat gcccttttta 180 aaagccggat ttcaggtttt tatcttaagg tattcagtga aagaagatgc cgtctggcc:c 240 aatccgctgg atgattatga acaggctatg gaattgatac gctccaaaga ggaggagtgg 300 aaattgtatg ccgataaggt ggcggttatt ggattttccg caggcgggca tttggcgggc 360 Sequence Listing (2) tgtgcggctg cgatgtcaaa gaatcgcccc aatgctgcag ttttgggata tgctgtgctg 420 ggagaggatg tcaaaggctg caatgtgagt gctccggatg tgattgcgtc agtggatgcc 480 gatacctgcc cctgcttcgt atttgcgacg cgtacggata atgtagttcc gattgaaaac 540 tccaccagat ttctgacggc tttggccgat gcaggcatcg catttgaaag tcatatttat 600 agttatgggc ctcatggatt ttcaacatgc gattcatctg tacaggcgct ggatggaatt 660 acaaaaagag cgcccgggtg ggttaatgat agtattggct ggctaaaaga aatccttgga 720 gattttggtg agaacggcct tgaagcgccg aagtgcccag cgagagtgaa cgatgataac 780 atgccttatt tatcagtgga ctgcagcttt aagcgtttgc agcaggttcc cgccgcaaag 840 cagatattgg atgggatttt ccaaaacatg cggagcagtg cggctgaaca gtatgcagat 900 aatcctatga tggatcttat gagcgagaat acagaaggcg gcggtctgtt ttcaatagta 960 agaaatatga ctttaagaga tatattaagc tttggacggg tgacagatga cattgtggcc 1020 gcaattaata gtcaacttga gaagatacct aatgtaagta aagcatcaaa ttga 1074 <210> 116 <211> 357 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 116 Met Arg Val Glu Glu Met Val Leu Asn Gly Glu Arg Asn Val Thr Leu Thr Ala Tyr Ile Gln Glu Val Ala Gly Glu Phe Arg Asn Ile Glu Lys Arg Pro Ala Ile Leu Ile Leu Pro Gly Gly Gly Tyr Gln Phe Cys Ser Asp Arg Glu Ala Asp Pro Val. Ala Met Pro Phe Leu Lys Ala Gly Phe Gln Val Phe Ile Leu Arg Tyr Ser Val Lys Glu Asp Ala Val Trp Pro Sequence Listing (2) Asn Pro Leu Asp Asp Tyr Glu Gln Ala Met Glu Leu Ile Arg Ser Lys Glu Glu Glu Trp Lys Leu Tyr Ala Asp Lys Val Ala Val Ile Gly Phe Ser Ala Gly Gly His Leu Ala Gly Cys Ala Ala Ala Met Ser Lys Asn Arg Pro Asn Ala Ala Val Leu Gly Tyr Ala Val Leu Gly Glu Asp Val Lys Gly Cys Asn Val Ser Ala Pro Asp Val Ile Ala Ser Val Asp Ala Asp Thr Cys Pro Cys Phe Val Phe Ala Thr Arg Thr Asp Asn Val Val Pro Ile Glu Asn Ser Thr Arg Phe Leu Thr Ala Leu Ala Asp Ala Gly Ile Ala Phe Glu Ser His Ile Tyr Ser Tyr Gly Pro His Gly Phe Ser Thr Cys Asp Ser Ser Val Gln Ala Leu Asp Gly Ile Thr Lys Arg Ala Pro Gly Trp Val Asn Asp Ser Ile Gly Trp Leu Lys Glu Ile Leu Gly Asp Phe Gly Glu Asn Gly Leu Glu Ala Pro Lys Cys Pro Ala Arg Val Asn Asp Asp Asn Met Pro Tyr Leu Ser Val Asp Cys Ser Phe Lys Arg Leu Gln Gln Val Pro Ala Ala Lys Gln Ile Leu Asp Gly Ile Phe Gln Asn Met Arg Ser Ser Ala Ala Glu Gln Tyr Ala Asp Asn Pro Met Met Sequence Listing (2) Asp Leu Met Ser Glu Asn Thr Glu Gly Gly Gly Leu Phe Ser Ile Val Arg Asn Met Thr Leu Arg Asp Ile Leu Ser Phe Gly Arg Val Thr Asp Asp Ile Val Ala Ala Ile Asn Ser Gln Leu Glu Lys Ile Pro Asn Val Ser Lys Ala Ser Asn <210> 117 <211> 900 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 117 atgataccgc aacaaacccc gccgccggaa acccccgttg acaaatcggc ccgggaacgc 60 atcggcgaaa ccaggaaagc gttcctgaat atgacggcgg agttcgcgac ttcgctggcg 120 gacattccga cgacggagta tgaccaggag ggtatccggg gaagctggat catgcccaat 180 gatgcggatt ccgaacgcgt gcttttgtat cttcacggcg gcggctacat agtcggaagc 240 gatgaaacgc cccgcgcgat aaccgcattt ctggcgcgcg aggcaaaagt gcggtgtttt 300 tccctggatt atccgcttgc ccccgagcat ccatttccgg ctgcgctcga tagcgcggtg 360 cgatcgtatg aaatgctgtt ggaaaaaggg ttcagccctg gaaacatcgt gctgggcggc 420 gattcggccg gcggcggcct ggcgcttgcc ctgttgctcg ccatccggga acacgggctg 480 ccgatgccgg cgggagcgta tctcctttcc ccgtggactg atttgacgca gagttttccc 540 acgcattcac gcaaggccga tgtcgaaatc agcattaccc ccgagctgct tgaggaagcg 600 gcagccagct atgcgggtga ttccgacaga accaatccgc ttatctcccc cgcattcggc 660 gagttccggg gattcccgcc gctcctgata caagttggtt cccatgaacg tctgttggac 720 gactccctga cggtggcgcg caatgccgcc ctcgccgatg tgccgacgac attgaaggta 780 Sequence Listing (2) tggcccggct atccccatgt gtttcaagtc tatcaccaga atctcgacgg cgccaaaaag 840 gcgttgaggg aagccgcgga attcatcacc ggagcgatgg ataaagagtt gcttcaataa 900 <210> 118 <211> 299 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 118 Met Ile Pro Gln Gln Thr Pro Pro Pro Glu Thr Pro Val Asp Lys Ser Ala Arg Glu Arg Ile Gly Glu Thr Arg Lys Ala Phe Leu Asn Met Thr Ala Glu Phe Ala Thr Ser Leu Ala Asp Ile Pro Thr Thr Glu Tyr Asp Gln Glu Gly Ile Arg Gly Ser Trp Ile Met Pro Asn Asp Ala Asp Ser Glu Arg Val Leu Leu Tyr Leu His Gly Gly Gly Tyr Ile Val Gly Ser Asp Glu Thr Pro Arg Ala Ile Thr Ala Phe Leu Ala Arg Glu Ala Lys Val Arg Cys Phe Ser Leu Asp Tyr Pro Leu Ala Pro Glu His Pro Phe Pro Ala Ala Leu Asp Ser Ala Val Arg Ser Tyr Glu Met Leu Leu Glu Lys Gly Phe Ser Pro Gly Asn Ile Val Leu Gly Gly Asp Ser Ala Gly Gly Gly Leu Ala Leu Ala Leu Leu Leu Ala Ile Arg Glu His Gly Leu Sequence Listing (2) Pro Met Pro Ala Gly Ala Tyr Leu Leu Ser Pro Trp Thr Asp Leu Thr Gln Ser Phe Pro Thr His Ser Arg Lys Ala Asp Val Glu Ile Ser Ile Thr Pro Glu Leu Leu Glu Glu Ala Ala Ala Ser Tyr Ala Gly Asp Ser Asp Arg Thr Asn Pro Leu Ile Ser Pro Ala Phe Gly Glu Phe Arg Gly Phe Pro Pro Leu Leu Ile Gln Val Gly Ser His Glu Arg Leu Leu Asp Asp Ser Leu Thr Val Ala Arg Asn Ala Ala Leu Ala Asp Val Pro Thr Thr Leu Lys Val Trp Pro Gly Tyr Pro His Val Phe Gln Val Tyr His Gln Asn Leu Asp Gly Ala Lys Lys Ala Leu Arg Glu Ala Ala Glu Phe Ile Thr Gly Ala Met Asp Lys Glu Leu Leu Gln <210> 119 <211> 810 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 119 atgacggcgg agcttgcgac ttcgctggcg gacgttccga cggcggagta tgaccaggag 60 ggtatccggg gaagctggat catgcccgat aaagcggatt ccgaacgcgt gcttttgtat 120 cttcacggcg gcggttacat agttggaagc gatgaaacgc ctcgcgcgat aaccgcattt 180 ctggcgcgcg aggcaaaagt gcggtgtttt tccctggatt atccgcttgc tcccgagcat 240 Sequence Listing (2) ccatttccgg ctgcgctcga tagcgcggtg cgatcgtatg aaatgctgtt ggaaaagggg 300 ttcagccccg gaaacatcgt gctgggcggc gactcggccg gcggcggcct ggcgcttgcc 360 ctgttgctcg ccatccggga acacgggctg ccgatgccgg cgggagcgta tctcctttcc 420 ccgtggactg atttgacgca gagttttccc acgcatttac gcaaggccga tgtcgaaatc 480 agtattgccc ccgagctgct cgaggaagcg gcagccagct atgcgggcga ttccgataga 540 accaatccgc ttatctcccc cgcattcggc gagttccggg gattcccgcc gctcctgata 600 caagttggtt cccatgaacg tctgttggac gattccctga cggtggcgcg caatgccgcc 660 ctcgccgatg tgccgacgac attgaaggta tggcccggct atccccatgt gtttcaactc 720 tatcatcaga atctcgacgg cgccaaaaag gcgttgaggg aagccgcgga attcatcacc 780 ggagcgatgg ataaagagtt gcttcaataa 810 <210> 120 <211> 269 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 120 Met Thr Ala Glu Leu Ala Thr Ser Leu Ala Asp Val Pro Thr Ala Glu Tyr Asp Gln Glu Gly Ile Arg Gly Ser Trp Ile Met Pro Asp Lys Ala Asp Ser Glu Arg Val Leu Leu Tyr Leu His Gly Gly Gly Tyr Ile Val Gly Ser Asp Glu Thr Pro Arg Ala Ile Thr Ala Phe Leu Ala Arg Glu Ala Lys Val Arg Cys Phe Ser Leu Asp Tyr Pro Leu Ala Pro Glu His Pro Phe Pro Ala Ala Leu Asp Ser Ala Val Arg Ser Tyr Glu Met Leu Sequence Listing (2) Leu Glu Lys Gly Phe Ser Pro Gly Asn Ile Val Leu Gly Gly Asp Ser Ala Gly Gly Gly Leu Ala Leu Ala Leu Leu Leu Ala Ile Arg Glu His Gly Leu Pro Met Pro Ala Gly Ala Tyr Leu Leu Ser Pro Trp Thr Asp Leu Thr Gln Ser Phe Pro Thr His Leu Arg Lys Ala Asp Val Glu Ile Ser Ile Ala Pro Glu Leu Leu Glu Glu Ala Ala Ala Ser Tyr Ala Gly Asp Ser Asp Arg Thr Asn Pro Leu Ile Ser Pro Ala Phe Gly Glu Phe Arg Gly Phe Pro Pro Leu Leu Ile Gln Val Gly Ser His Glu Arg Leu Leu Asp Asp Ser Leu Thr Val Ala Arg Asn Ala Ala Leu Ala Asp Val Pro Thr Thr Leu Lys Val Trp Pro Gly Tyr Pro His Val Phe Gln Leu Tyr His Gln Asn Leu Asp Gly Ala Lys Lys Ala Leu Arg Glu Ala Ala Glu Phe Ile Thr Gly Ala Met. Asp Lys Glu Leu Leu Gln <210> 121 <211> 918 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 121 Sequence Listing (2) atgcagcgta ttgctgatca gatgaaacgt gcaggtcata aacccatgta tgagctttca 60 ccgcagcagg cgcgggaata ctatgagcgc aatgccagta ttttggattt gccagcagca 120 tcgttgccgc gtgtagagaa tttttatatt ccatcaacag atggcgagtt attgccggcc 180 agattgtatg caccgtcaga agagaagggc ttgcctgttc tcttgttttt tcatgggggt 240 gggtttaccg ttggcagtat tgacacgcac gatagtctgt gccgccagct tgcgttgctc 300 agtggtgttg ccgtggtgtc gctggattac cggctggcac cggaatataa attcccggtt 360 gcagtggatg acgcatgggc ggcctgccag tggctgaacc agaatgggca gactctgggg 420 ctggatggtt tccggctggc tgtcgggggc gatagtgccg ggggaacctt ggccgccgtg 480 tgtgccatta tggcacgcga tgcgcagttg cctgtggcat tgcaggtgct gttttacccg 540 ggcactacag cacaccagga taccgagtcg cacacggtgt atgccgataa cgcgattatt 600 cctgaagtgc tgattacctg gttcttccac cagtatattg aagatgggca gcgtgaagac 660 tggcgctttg caccgctgtt gtgtgacgac ttgcaaggtg ttgcacccgc ctggatggga 720 ctggcggaat gtgacccgtt ggtggatgaa ggtgtcgcat atgctgaccg gctgcgtttt 780 gccggagtgc cggtggaact ggagctctat aaaggtgtga cgcacgagtt tgccaaattc 840 gggcaggcga taccggaagc cctggcagcc cagaaagctg cggcacaggc attggcacag 900 gtatttgatg tggtttaa 918 <210> 122 <211> 305 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 122 Met Gln Arg Ile Ala Asp Gln Met Lys Arg Ala Gly His Lys Pro Met Tyr Glu Leu Ser Pro Gln Gln Ala Arg Glu Tyr Tyr Glu Arg Asn Ala Ser Ile Leu Asp Leu Pro Ala Ala Ser Leu Pro Arg Val Glu Asn Phe Sequence Listing (2) Tyr Ile Pro Ser Thr Asp Gly Glu Leu Leu Pro Ala Arg Leu Tyr Ala Pro Ser Glu Glu Lys Gly Leu Pro Val Leu Leu Phe Phe His Gly Gly Gly Phe Thr Val Gly Ser Ile Asp Thr His Asp Ser Leu Cys Arg Gln Leu Ala Leu Leu Ser Gly Val Ala Val Val Ser Leu Asp Tyr Arg Leu Ala Pro Glu Tyr Lys Phe Pro Val Ala Val Asp Asp Ala Trp Ala Ala Cys Gln Trp Leu Asn Gln Asn Gly Gln Thr Leu Gly Leu Asp Gly Phe Arg Leu Ala Val Gly Gly Asp Ser Ala Gly Gly Thr Leu Ala Ala Val Cys Ala Ile Met Ala Arg Asp Ala Gln Leu Pro Val Ala Leu Gln Val Leu Phe Tyr Pro Gly Thr Thr Ala His Gln Asp Thr Glu Ser His Thr Val Tyr Ala Asp Asn Ala Ile Ile Pro Glu Val Leu Ile Thr Trp Phe Phe His Gin Tyr Ile Glu Asp Gly Gln Arg Glu Asp Trp Arg Phe Ala Pro Leu Leu Cys Asp Asp Leu Gln Gly Val Ala Pro Ala Trp Met Gly Leu Ala Glu Cys Asp Pro Leu Val Asp Glu Gly Val Ala Tyr Ala Asp Arg Leu Arg Phe Ala Gly Val Pro Val Glu Leu Glu Leu Tyr Lys Gly Sequence Listing (2) Val Thr His Glu Phe Ala Lys Phe Gly Gln Ala Ile Pro Glu Ala Leu Ala Ala Gln Lys Ala Ala Ala Gln Ala Leu Ala Gln Val Phe Asp Val Val <210> 123 <211> 1242 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 123 atgaatgttg ccaccaaaat ggctcgtgcc gaagatgtgg gtctgaattc cgaccgcttq 60 gcgcaaattc ccaaattcat gcagtcctat ctggatagcg gcaaactggc aggcgtgtcc 120 acgctggtct cgcgtcgcgg tgaagtggcg cattttgaat gtatcggcgt gaaggacaag 180 gacagcaaag aaccgctgac ccatgacacg atcttccgca tctactcgat gaccaagccg 240 atcacgagcc ttgcgctgat gatgctttat gaagaaagca agttccagct gaaccatccg 300 gtggccaaat atctgcccga attcgaaaag ctgaaagtct gggcgggcgg caccaaggaa 360 aagttcgaca caaagccttg cgaacgcgcg atgacgatcc gcgatttgct gacgcataca 420 tccggcctga cctatggctt catgatggcg catcctgtgg acgccatgta tcgcgcggca 480 ggcattgacg gggcaaatac tgttggtatg acgctcaagg aatttgtgga gaagctgtcg 540 gaaattccgc tgcttttctc gcccggtaca cagtggagct attcggttgc gaccgatgtt 600 tgcgggcgtc tggtagaagt gctgtcgggc atgtcgctcg acaagttctt tgagagccgc 660 atcttcaagc cgcttggcat ggttgatacg ggtttccatg tgcctgccaa taagctggat 720 cgttttgcca gcaactattt caaaaatcct gtcacccata aagaagggct gatggacaag 780 ggcgacgcga caagcactta cgccaaggcc ccctcatttc tgtcgggcgg tggcggactt 840 gtctcgacaa tgagcgacta ttggcgcttc tgccaaatgc tgctgaatgg cggcgagttc 900 Sequence Listing (2) aacgggcatc gctttgtcag ccgcaagact gtggatttca tgcgcatgaa ccacttgccc 960 ggcgacaaaa cgatggcaga gatgtcatcc tcggcattct cggaactggc gtccgaaggt 1020 acgggcttcg gcctcggctt tgctgtgacg cttgatcctg cagaactgca acagatcggc 1080 agccatggaa gcttctcgtg gggcggtgcg gcatcgacat atttctgggt tgatccggaa 1140 gaggacatga tcgctatcct gatgacgcag ctttatccat cttcgactta tccgctgcgg 1200 ccacaactgg cgcagttggt ttatgcggcg atcgaggatt ga 1242 <210> 124 <211> 413 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 124 Met Asn Val Ala Thr Lys Met Ala Arg Ala Glu Asp Val Gly Leu Asn Ser Asp Arg Leu Ala Gln Ile Pro Lys Phe Met Gln Ser Tyr Leu Asp Ser Gly Lys Leu Ala Gly Val Ser Thr Leu Val Ser Arg Arg Gly Glu Val Ala His Phe Glu Cys Ile Gly Val Lys Asp Lys Asp Ser Lys Glu Pro Leu Thr His Asp Thr Ile Phe Arg Ile Tyr Ser Met Thr Lys Pro Ile Thr Ser Leu Ala Leu Met Met Leu Tyr Glu Glu Ser Lys Phe Gln Leu Asn His Pro Val Ala Lys Tyr Leu Pro Glu Phe Glu Lys Leu Lys Val Trp Ala Gly Gly Thr Lys Glu Lys Phe Asp Thr Lys Pro Cys Glu Sequence Listing (2) Arg Ala Met Thr Ile Arg Asp Leu Leu Thr His Thr Ser Gly Leu Thr Tyr Gly Phe Met Met Ala His Pro Val Asp Ala Met Tyr Arg Ala Ala Gly Ile Asp Gly Ala Asn Thr Val Gly Met Thr Leu Lys Glu Phe Val Glu Lys Leu Ser Glu Ile Pro Leu Leu Phe Ser Pro Gly Thr Gln Trp Ser Tyr Ser Val Ala Thr Asp Val Cys Gly Arg Leu Val Glu Val Leu Ser Gly Met Ser Leu Asp Lys Phe Phe Glu Ser Arg Ile Phe Lys Pro Leu Gly Met Val Asp Thr Gly Phe His Val Pro Ala Asn Lys Leu Asp Arg Phe Ala Ser Asn Tyr Phe Lys Asn Pro Val Thr His Lys Glu Gly Leu Met Asp Lys Gly Asp Ala Thr Ser Thr Tyr Ala Lys Ala Pro Ser Phe Leu Ser Gly Gly Gly Gly Leu Val Ser Thr Met Ser Asp Tyr Trp Arg Phe Cys Gln Met Leu Leu Asn Gly Gly Glu Phe Asn Gly His Arg Phe Val Ser Arg Lys Thr Val Asp Phe Met Arg Met Asn His Leu Pro Gly Asp Lys Thr Met Ala Glu Met Ser Ser Ser Ala Phe Ser Glu Leu Ala Ser Glu Gly Thr Gly Phe Gly Leu Gly Phe Ala Val Thr Leu Asp Sequence Listing (2) Pro Ala Glu Leu Gln Gln Ile Gly Ser His Gly Ser Phe Ser Trp Gly Gly Ala Ala Ser Thr Tyr Phe Trp Val Asp Pro Glu Glu Asp Met Ile Ala Ile Leu Met Thr Gln Leu Tyr Pro Ser Ser Thr Tyr Pro Leu Arg Pro Gln Leu Ala Gln Leu Val Tyr Ala Ala Ile Glu Asp <210> 125 <211> 876 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 125 atgttccgcg agacccggcc ggcgtcgacg ccgacgccgc cggcgatcgg cgccgtccgc 60 gacgagatcg ccgagacggc ctccggttcg atcccgctgc gcgtgtaccg gccggccagc 120 gtggcgtcgg acgcgaccct cccggggctg gtctacttcc acggcggggg ctgggtgatc 180 ggggacctcg acacgcacga cgtgctctgc cgtcagctca ccgccggggc ggggctcacc 240 gtcgtctcgg tggactaccg gctcgctccc gagcacccgt ttccggctgc ggtcgacgac 300 gcctgggcgg ccacgcgctg ggtcgtcgag cgcgcggacc acctggggat cgacgcgcgc 360 cggcttgccg tgggcgggga cagcgccggc ggcaacctcg ccgccgtgac gacgctgctc 420 gcgcgggacg cgggggcgcc gtcgatcgcc taccaggtgc tgctctaccc cgtgaccgac 480 gtcgccgccg aggcgccgtc gtacgacgcc ttcgccgacg gctacctgct gacccgcgcc 540 ggcatgcgct ggttcatcga gcactacctg ccgtcccggc gcgacgccgc cgactggcgc 600 gtctcgccgc tgcgcgcccc ctcgctcgcg gggctgccgc cggccctgat cgtgaccgcc 660 gggttcgatc ccctctgcga cgagggcgag gcctacgcgc gcgcgctccg cggtgccggc 720 gtccgcgtgg actacgtccg ctatggcggc atgatccacg gcttcgtgcc catgggccgc 780 Sequence Listing (2) ctcatcgaga cgggcaaccg tgctgtcgcg cacatcgccg cctcgctgcg cgacgccctg 840 accgcgacgg gccaggcggc ggcgccggtg agataa 876 <210> 126 <211> 291 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 126 Met Phe Arg Glu Thr Arg Pro Ala Ser Thr Pro Thr Pro Pro Ala Ile Gly Ala Val Arg Asp Glu Ile Ala Glu Thr Ala Ser Gly Ser Ile Pro Leu Arg Val Tyr Arg Pro Ala Ser Val Ala Ser Asp Ala Thr Leu Pro Gly Leu Val Tyr Phe His Gly Gly Gly Trp Val Ile Gly Asp Leu Asp Thr His Asp Val Leu Cys Arg Gln Leu Thr Ala Gly Ala Gly Leu Thr Val Val Ser Val Asp Tyr Arg Leu Ala Pro Glu His Pro Phe Pro Ala Ala Val Asp Asp Ala Trp Ala Ala Thr Arg Trp Val Val Glu Arg Ala Asp His Leu Gly Ile Asp Ala Arg Arg Leu Ala Val Gly Gly Asp Ser Ala Gly Gly Asn Leu Ala Ala Val Thr Thr Leu Leu Ala Arg Asp Ala Gly Ala Pro Ser Ile Ala Tyr Gln Val Leu Leu Tyr Pro Val Thr Asp Sequence Listing (2) Val Ala Ala Glu Ala Pro Ser Tyr Asp Ala Phe Ala Asp Gly Tyr Leu Leu Thr Arg Ala Gly Met Arg Trp Phe Ile Glu His Tyr Leu Pro Ser Arg Arg Asp Ala Ala Asp Trp Arg Val Ser Pro Leu Arg Ala Pro Ser Leu Ala Gly Leu Pro Pro Ala Leu Ile Val Thr Ala Gly Phe Asp Pro Leu Cys Asp Glu Gly Glu Ala Tyr Ala Arg Ala Leu Arg Gly Ala Gly Val Arg Val Asp Tyr Val Arg Tyr Gly Gly Met Ile His Gly Phe Val Pro Met Gly Arg Leu Ile Glu Thr Gly Asn Arg Ala Val Ala His Ile Ala Ala Ser Leu Arg Asp Ala Leu Thr Ala Thr Gly Gln Ala Ala Ala Pro Val Arg <210> 127 <211> 990 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 127 atgggcgcgt tcgaggccac cagcgcgcag ggtgacgtcg ccagccgcga cgaactgct.g 60 gccgaggcgg ccagcgacga agcggtggcc cagcgggaga tgctcaccgc cttcctcggc 120 atgtgcgaca ccgaggagat cgcgccgtcg gcggggctca cggtctccga gcaccgcgtc 180 gcctctcagc ccgacggcaa caagatcaac atccggttca tccggcccga gggcgacgac 240 Sequence Listing (2) gtgctgccgt gcgtgtacta catccacggc ggtggcatgg cgtcgatgtc gtgctatgac 300 ggcaactacc gggcgtgggg ccggatcatc gccgcccagg gcgtggcggt tgccatggtc 360 gacttccgca acgcgctcac accgtcgtcg gccgaggagg tggccccgtt ccccgctggg 420 ctcaacgact gcgtgtcggg gctcaagtgg gtcgcggcca acgccgccga tctgcgcatc 480 gacccggccc gcatcgtcgt ggccggcgag agcggcggcg ggaacctcac cctcgccacc 540 ggcctgaagc tcaagcgcga cggcgacctc gggctgatca agggtctcta tgccctctgc 600 ccctacattg ccggcgagtg gccgctgccg cagaacccgt cgtcgaccga gaacgagggc 660 atcctcctgc acctgcacaa caaccgcggc cgcatgacgt acggcatcga ggagttcgag 720 gcgcggaacc cgctggcgtg gcccggcttc gccactgaag acgacgtcgc cggcctcgtg 780 cccaccatca tcagcgtgaa cgagtgcgac ccgctgcgcg acgagggcgt cggcttctac 840 cgcctcctcc tccgcgcggg tgtgcccacc cggtgccgcc aggtgatggg caccatccac 900 ggcaccgaga tcttcccgat gctgtgcccc gacgtgagcc gggacaccgc ggccagcatc 960 gccgacttcg cccgcaacgc cgggtcctga 990 <210> 128 <211> 329 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 128 Met Gly Ala Phe Glu Ala Thr Ser Ala Gln Gly Asp Val Ala Ser Arg Asp Glu Leu Leu Ala Glu Ala Ala Ser Asp Glu Ala Val Ala Gln Arg Glu Met Leu Thr Ala Phe Leu Gly Met Cys Asp Thr Glu Glu Ile Ala Pro Ser Ala Gly Leu Thr Val Ser Glu His Arg Val Ala Ser Gln Pro Asp Gly Asn Lys Ile Asn Ile Arg Phe Ile Arg Pro Glu Gly Asp Asp Sequence Listing (2) Val Leu Pro Cys Val Tyr Tyr Ile His Gly Gly Gly Met Ala Ser Met Ser Cys Tyr Asp Gly Asn Tyr Arg Ala Trp Gly Arg Ile Ile Ala Ala Gln Gly Val Ala Val Ala Met Val Asp Phe Arg Asn Ala Leu Thr Pro Ser Ser Ala Glu Glu Val Ala Pro Phe Pro Ala Gly Leu Asn Asp Cys Val Ser Gly Leu Lys Trp Val Ala Ala Asn Ala Ala Asp Leu Arg Ile Asp Pro Ala Arg Ile Val Val Ala Gly Glu Ser Gly Gly Gly Asn Leu Thr Leu Ala Thr Gly Leu Lys Leu Lys Arg Asp Gly Asp Leu Gly Leu Ile Lys Gly Leu Tyr Ala Leu Cys Pro Tyr Ile Ala Gly Glu Trp Pro Leu Pro Gln Asn Pro Ser Ser Thr Glu Asn Glu Gly Ile Leu Leu His Leu His Asn Asn Arg Gly Arg Met Thr Tyr Gly Ile Glu Glu Phe Glu Ala Arg Asn Pro Leu Ala Trp Pro Gly Phe Ala Thr Glu Asp Asp Val Ala Gly Leu Val Pro Thr Ile Ile Ser Val Asn Glu Cys Asp Pro Leu Arg Asp Glu Gly Val Gly Phe Tyr Arg Leu Leu Leu Arg Ala Gly Val Sequence Listing (2) Pro Thr Arg Cys Arg Gln Val Met Gly Thr Ile His Gly Thr Glu Ile Phe Pro Met Leu Cys Pro Asp Val Ser Arg Asp Thr Ala Ala Ser Ile Ala Asp Phe Ala Arg Asn Ala Gly Ser <210> 129 <211> 756 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 129 gtgcttgtcg tgcatggttt tgtggaaaac ttcctctatt ttaccgaata ctatgccgac 60 ccagaccttg agctgattct gctgaccagc gcggattacc atgtgcctgt cacccaggcc 120 acccatcagc aagctgcctg ggccaaggca cccaaagagc gcctgggaac tattgcctat 180 gatgctgagg ttttaattct ggggttggaa cacctggtca atggccagga agtgcgtgtg 240 cacggccatt cccgcggtgg tgctgtggta ctggaagccg cccgtcgtcg cccggatcta 300 tttgagcagg ttgaagtgat tcttgaggca cccgtgttgc ctcagggcaa accttatgcc 360 gaactgcccc ccagcgtgcg ctggttcctg ccctttgtac tgcccttctg gcaacagcaa 420 cccatctcgc cccgcaacaa ggccatctgg gggccgctgg atgatcggcg caaacgctct 480 ctgatcatgg ggctgccgtt caaccccaaa cattgcagca tcctcatggc caacattaag 540 gatatggcgg aatggatgcc gggcaatggc agtgagctgt atcagaacgt taaacgtggc 600 gccattctag tgcctggcaa tgacaaggtg ctggagcccc gcgccatgct cgccagtgcc 660 cgacaggcag aaaatctgca agtgattgaa atcgaaaacg gcagtcattt tgttatccc:t 720 gaccaccctg attcgattcc gcccttgccg gcatga 756 <210> 130 <211> 251 <212> PRT
<213> Unknown Sequence Listing (2) <220>
<223> Obtained from an environmental sample <400> 130 Met Leu Val Val His Gly Phe Val Glu Asn Phe Leu Tyr Phe Thr Glu Tyr Tyr Ala Asp Pro Asp Leu Glu Leu Ile Leu Leu Thr Ser Ala Asp Tyr His Val Pro Val Thr Gln Ala Thr His Gln Gln Ala Ala Trp Ala Lys Ala Pro Lys Glu Arg Leu Gly Thr Ile Ala Tyr Asp Ala Glu Val Leu Ile Leu Gly Leu Glu His Leu Val Asn Gly Gln Glu Val Arg Val His Gly His Ser Arg Gly Gly Ala Val Val Leu Glu Ala Ala Arg Arg Arg Pro Asp Leu Phe Glu Gln Val Glu Val Ile Leu Glu Ala Pro Val Leu Pro Gln Gly Lys Pro Tyr Ala Glu Leu Pro Pro Ser Val Arg Trp Phe Leu Pro Phe Val Leu Pro Phe Trp Gln Gln Gln Pro Ile Ser Pro Arg Asn Lys Ala Ile Trp Gly Pro Leu Asp Asp Arg Arg Lys Arg Ser Leu Ile Met Gly Leu Pro Phe Asn Pro Lys His Cys Ser Ile Leu Met Ala Asn Ile Lys Asp Met Ala Glu Trp Met Pro Gly Asn Gly Ser Glu Leu Tyr Gln Asn Val Lys Arg Gly Ala Ile Leu Val Pro Gly Asn Asp Sequence Listing (2) Lys Val Leu Glu Pro Arg Ala Met Leu Ala Ser Ala Arg Gln Ala Glu Asn Leu Gin Val Ile Glu Ile Glu Asn Gly Ser His Phe Val Ile Pro Asp His Pro Asp Ser Ile Pro Pro Leu Pro Ala <210> 131 <211> 813 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 131 atggctaccc aacctcgcga agagatgatc tcggtcggcg ggatcaaggt gcacacactg 60 atcgggggac acggcgaccc ggtactcgtt ctgcacgggg ccggcggatc gctcggctgg 120 cggcgctggc tcgaccggct cgccgaaaac ttcaccgtgt acgcgccgtc gcaccccggc 180 tacgaccggt cggacagtgc cgactggatg gagagcgtgc gggatatcgc ccgcttttac 240 ctctggttcc tcgatgtcgt gggacaggac cgggtccacg tgatcggttc ctccatgggc 300 ggctggacgg ccgctgaatt ggccgtaatg aatcctcacg tcgtgaaccg gctggtgttg 360 gtcgatccgg tcggcctgaa gccggagcag ggagagatcc tcgacatctt tttcctttcc 420 ccggaggaag tgttcgcgaa ggcctactac aacgcggagc aggccccgga gtacgcggaa 480 ctctatggtc agccgccgac cgccgagcag cgggacattc agatgcgcaa ccgggagat.g 540 gcggcccgga tcacctggaa gccgtacatg cacaatcccc gcctgccgca cttcctgccg 600 cgggtgacgg tgccgacgct gatcgtctgg ggccgggaag acgagatcgt cccactggag 660 tgtggggaac agtaccagcg gctcctgccg aacgcgacgc tccgcgtgat cgaccagtgc 720 gggcactcgc cccagatcga gaagccggat gagttcctga gcctcgtcac cgagtttct:g 780 ggcgtggcca gtccggccgg cgcaaagcag tga 813 <210> 132 Sequence Listing (2) <211> 270 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 132 Met Ala Thr Gln Pro Arg Glu Glu Met Ile Ser Val Gly Gly Ile Lys Val His Thr Leu Ile Gly Gly His Gly Asp Pro Val Leu Val Leu His Gly Ala Gly Gly Ser Leu Gly Trp Arg Arg Trp Leu Asp Arg Leu Ala Glu Asn Phe Thr Val Tyr Ala Pro Ser His Pro Gly Tyr Asp Arg Ser Asp Ser Ala Asp Trp Met Glu Ser Val Arg Asp Ile Ala Arg Phe Tyr Leu Trp Phe Leu Asp Val Val Gly Gln Asp Arg Val His Val Ile Gly Ser Ser Met Gly Gly Trp Thr Ala Ala Glu Leu Ala Val Met Asn Pro His Val Val Asn Arg Leu Val Leu Val Asp Pro Val Gly Leu Lys Pro Glu Gln Gly Glu Ile Leu Asp Ile Phe Phe Leu Ser Pro Glu Glu Val Phe Ala Lys Ala Tyr Tyr Asn Ala Glu Gln Ala Pro Glu Tyr Ala Glu Leu Tyr Gly Gln Pro Pro Thr Ala Glu Gln Arg Asp Ile Gln Met Arg Asn Arg Glu Met Ala Ala Arg Ile Thr Trp Lys Pro Tyr Met His Asn Sequence Listing (2) Pro Arg Leu Pro His Phe Leu Pro Arg Val Thr Val Pro Thr Leu Ile Val Trp Gly Arg Glu Asp Glu Ile Val Pro Leu Glu Cys Gly Glu Gln Tyr Gln Arg Leu Leu Pro Asn Ala Thr Leu Arg Val Ile Asp Gln Cys Gly His Ser Pro Gln Ile Glu Lys Pro Asp Glu Phe Leu Ser Leu Val Thr Glu Phe Leu Gly Val Ala Ser Pro Ala Gly Ala Lys Gln <210> 133 <211> 990 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 133 atgaaacgca taacggggat cttgaagggt gtcggggtaa ttttgctggt ctttttggc:a 60 gggctactgg tgattccgtt gatct.tgccg gtacctcctt tacaggatat ccgggatgtc 120 agtgaattaa aggacccgga cagccagttc attgaaatcc aggggattca ggttcattat 180 aaaaaatatg gctcgggtga acctacgatg attttgcttc atgggtttgg tgccagtac:g 240 ttttcctggc gtgaggtcat ggcaccgctt tcgaatcatg gtacggtcat tgcttacgat 300 cgccctgcgt ttggattgac cagccgtcct ttgcctgggg aatggcggga taagaacccg 360 tacagcgtgg aaggtaacat tgcccttttg gttgagttga tggatcgtct tggggtgaa.a 420 caggcgatcc tgattggaaa ttcagcgggt ggaaggctgg caatgcagat ggctctggct 480 catccggagc gtgtttccgg attaattctg gtggatgccg ctatctatca ggggggcggg 540 gtgcagtctt caatcctccg tgccctcatg aatactcctc aattgaaccg cattggacct 600 tattttttga gattcgcttt tgcaggggaa cagggtcaat cgctgatttc gatggcttgg 660 Sequence Listing (2) catgatcctt cgaaattgac acccgagatt atcgaggggt atcgcaaacc tctccatatg 720 gaaaactggg atcgtgccct atgggaattc acgaaatcag gtagcggtag cgaagatttg 780 tccctccgtt ttaatgagtt gagcatgccc gtgctggttg tgaccggaga tgatgatcgc 840 attgtaccta catcgctcag tttacagttg gctgaagata ttcctcaggc tcaattggtg 900 gtattttcga gttgtggtca tgttccacag gaagaatgcc ctgatcagtt cttgagagcg 960 gtagaagatt ttctcggtaa gaatagataa 990 <210> 134 <211> 329 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 134 Met Lys Arg Ile Thr Gly Ile Leu Lys Gly Val Gly Val Ile Leu Leu Val Phe Leu Ala Gly Leu Leu Val Ile Pro Leu Ile Leu Pro Val Pro Pro Leu Gln Asp Ile Arg Asp Val Ser Glu Leu Lys Asp Pro Asp Ser Gln Phe Ile Glu Ile Gln Gly Ile Gln Val His Tyr Lys Lys Tyr Gly Ser Gly Glu Pro Thr Met Ile Leu Leu His Gly Phe Gly Ala Ser Thr Phe Ser Trp Arg Glu Val Met. Ala Pro Leu Ser Asn His Gly Thr Val Ile Ala Tyr Asp Arg Pro Ala Phe Gly Leu Thr Ser Arg Pro Leu Pro Gly Glu Trp Arg Asp Lys Asn Pro Tyr Ser Val Glu Gly Asn Ile Ala Sequence Listing (2) Leu Leu Val Glu Leu Met Asp Arg Leu Gly Val Lys Gln Ala Ile Leu Ile Gly Asn Ser Ala Gly Gly Arg Leu Ala Met Gln Met Ala Leu Ala His Pro Glu Arg Val Ser Gly Leu Ile Leu Val Asp Ala Ala Ile Tyr Gln Gly Gly Gly Val Gln Ser Ser Ile Leu Arg Ala Leu Met Asn Thr Pro Gln Leu Asn Arg Ile Gly Pro Tyr Phe Leu Arg Phe Ala Phe Ala Gly Glu Gln Gly Gln Ser Leu Ile Ser Met Ala Trp His Asp Pro Ser Lys Leu Thr Pro Glu Ile Ile Glu Gly Tyr Arg Lys Pro Leu His Met Glu Asn Trp Asp Arg Ala Leu Trp Glu Phe Thr Lys Ser Gly Ser Gly Ser Glu Asp Leu Ser Leu Arg Phe Asn Glu Leu Ser Met Pro Val Leu Val Val Thr Gly Asp Asp Asp Arg Ile Val Pro Thr Ser Leu Ser Leu Gln Leu Ala Glu Asp Ile Pro Gln Ala Gln Leu Val Val Phe Ser Ser Cys Gly His Val Pro Gln Glu Glu Cys Pro Asp Gln Phe Leu Arg Ala Val Glu Asp Phe Leu Gly Lys Asn Arg <210> 135 Sequence Listing (2) <211> 849 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 135 gtgcaagacc tccgtagaat tatgaaggag tacagattcc cggaaacccc agaagaggtc 60 gaatcaacta aggattatta cttggaacag aacggcgaaa gaatcaggat gaggctgtac 120 accccaatag gaggctccga ctcccttata gtgtattacc atggcggggg ctttgtattc 180 ggggacataa aactctatga caatgtatgc aggaagatcg cgacaagatc atcctccaag 240 gttctctctg taaagtacag gctggccccg gagcataaat ttccggcagc agtagatgat 300 gcctattctg cgtataaatg ggcgaggaag aacgcacgcg agatcggcat aaaggaaggc 360 ctcatagcag tgggagggga tagcgctggc ggaaaccttg cggctgttgt tacgttaaag 420 gcgaaggatg agaaatacga tcagccaatg ctccaggtcc ttttctatcc agcccttggt 480 ccagacttct tcagcgaatc cctgagggaa tatggcgaag ggtatttcct tacaaagaag 540 cagatggaca acttcggggc aatgtatttc aggaacccgg ctgatgcctt cagtccctac 600 ttcgcgccta tcctttacgg aaacttttcc ggcgttgcgg aggccataat tgtaacggca 660 gagcacgatc cccttaggga tcagggggag atgtacataa aagagctcta tgactccggc 720 gtccaggtca caggaataag ggcaaagggc atgatacatg gtttcctgag cttttcggc:c 780 attgtcccgg ccgcggacaa cctattcaca atggtatggg cgatggtagg ttcaaaactc 840 aggaaataa 849 <210> 136 <211> 282 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 136 Met Gln Asp Leu Arg Arg Ile Met Lys Glu Tyr Arg Phe Pro Glu Thr Pro Glu Glu Val Glu Ser Thr Lys Asp Tyr Tyr Leu Glu Gln Asn Gly Sequence Listing (2) Glu Arg Ile Arg Met Arg Leu Tyr Thr Pro Ile Gly Gly Ser Asp Ser Leu Ile Val Tyr Tyr His Gly Gly Gly Phe Val Phe Gly Asp Ile Lys Leu Tyr Asp Asn Val Cys Arg Lys Ile Ala Thr Arg Ser Ser Ser Lys Val Leu Ser Val Lys Tyr Arg Leu Ala Pro Glu His Lys Phe Pro Ala Ala Val Asp Asp Ala Tyr Ser Ala Tyr Lys Trp Ala Arg Lys Asn Ala Arg Glu Ile Gly Ile Lys Glu Gly Leu Ile Ala Val Gly Gly Asp Ser Ala Gly Gly Asn Leu Ala Ala Val Val Thr Leu Lys Ala Lys Asp Glu Lys Tyr Asp Gln Pro Met Leu Gln Val Leu Phe Tyr Pro Ala Leu Gly Pro Asp Phe Phe Ser Glu Ser Leu Arg Glu Tyr Gly Glu Gly Tyr Phe Leu Thr Lys Lys Gln Met Asp Asn Phe Gly Ala Met Tyr Phe Arg Asn Pro Ala Asp Ala Phe Ser Pro Tyr Phe Ala Pro Ile Leu Tyr Gly Asn Phe Ser Gly Val Ala Glu Ala Ile Ile Val Thr Ala Glu His Asp Pro Leu Arg Asp Gln Gly Glu Met Tyr Ile Lys Glu Leu Tyr Asp Ser Gly Sequence Listing (2) Val Gln Val Thr Gly Ile Arg Ala Lys Gly Met Ile His Gly Phe Leu Ser Phe Ser Ala Ile Val Pro Ala Ala Asp Asn Leu Phe Thr Met Val Trp Ala Met Val Gly Ser Lys Leu Arg Lys <210> 137 <211> 924 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 137 atggcttcca ttcaatctcg tattttcact gggttattgc gtattgcgtc aggctaccgc 60 tctcgaagcg atcgcacctc cgcgcaaaca gccgtgaaca agtcccgtgc ctttacgcgg 120 ctgatgacct tgccccagct tccgggaacg cgatccatcc acgccgacat tccgaacctt 180 cgcgcaacgt ggcatgtggg gaagcacgta cgggacgatg tgcgcatcgt ctatttccac 240 ggcggagcct ataccgcagg gtctgtcgcg acgcatcgac acttcacctc gatgctcgc.a 300 aaactcagcc atatccccgt gctttctgtc gactatcgcc tcgcaccaga acatcctttt 360 cctgcgggct ttgatgatgc aattgccgcc tatcaatggg ccaaagagca cgggccagta 420 ggaaagagcg aagcgcaaca tatcgtaatt gcaggtgatt ctgcgggcgg aggcctcagt 480 gcggcgatcg cgatgcaact gcgcgatgca ggacagctcc tagacggcgg cctcatgctg 540 ctttcgccgt ggatggatct gacctgtagc tccggcgcag aacatcgcat cggacatctt 600 gatgccatgc tcaatgcgga ccacgcgcgt atcatggcga gtctttacgc aggaacccat 660 gacttgaccg acccacgact ttcgccgctt tatggagact ttgcgggtct tccgcccatg 720 ttcgtcgcca ttggcggacg agaaatcgtg ctcgatgagg tggttcatgc ccttgaaaaa 780 gcgcgcgcag cgggcgtgga agttaccctc gaacgtaatg aagaaatgtt ccacgtttgg 840 ccagtgatgt tccatctcgt ccccgaagga cgcgcaagct tagagcgcat ggtcgattgg 900 ctgaattgga agtttcctgc atag 924 Sequence Listing (2) <210> 138 <211> 307 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 138 Met Ala Ser Ile Gln Ser Arg Ile Phe Thr Gly Leu Leu Arg Ile Ala Ser Gly Tyr Arg Ser Arg Ser Asp Arg Thr Ser Ala Gln Thr Ala Val Asn Lys Ser Arg Ala Phe Thr Arg Leu Met Thr Leu Pro Gln Leu Pro Gly Thr Arg Ser Ile His Ala Asp Ile Pro Asn Leu Arg Ala Thr Trp His Val Gly Lys His Val Arg Asp Asp Val Arg Ile Val Tyr Phe His Gly Gly Ala Tyr Thr Ala Gly Ser Val Ala Thr His Arg His Phe Thr Ser Met Leu Ala Lys Leu Ser His Ile Pro Val Leu Ser Val Asp Tyr Arg Leu Ala Pro Glu His Pro Phe Pro Ala Gly Phe Asp Asp Ala Ile Ala Ala Tyr Gln Trp Ala Lys Glu His Gly Pro Val Gly Lys Ser Glu Ala Gln His Ile Val Ile Ala Gly Asp Ser Ala Gly Gly Gly Leu Ser Ala Ala Ile Ala Met Gln Leu Arg Asp Ala Gly Gln Leu Leu Asp Gly Sequence Listing (2) Gly Leu Met Leu Leu Ser Pro Trp Met Asp Leu Thr Cys Ser Ser Gly Ala Glu His Arg Ile Gly His Leu Asp Ala Met Leu Asn Ala Asp His Ala Arg Ile Met Ala Ser Leu Tyr Ala Gly Thr His Asp Leu Thr Asp Pro Arg Leu Ser Pro Leu Tyr Gly Asp Phe Ala Gly Leu Pro Pro Met Phe Val Ala Ile Gly Gly Arg Glu Ile Val Leu Asp Glu Val Val His Ala Leu Glu Lys Ala Arg Ala Ala Gly Val Glu Val Thr Leu Glu Arg Asn Glu Glu Met Phe His Val Trp Pro Val Met Phe His Leu Val Pro Glu Gly Arg Ala Ser Leu Glu Arg Met Val Asp Trp Leu Asn Trp Lys Phe Pro Ala <210> 139 <211> 747 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 139 atgaaaatta gacaaccaca accatttaca tttgaagcag gaaaaagggc cgttttatta 60 ttgcatggat ttacaggtca ttcggcagat gtacgcatgc ttggtcgcta tttagaggaa 120 aaaggttata cctctcatgc gccaatttat cgtggccatg gtcttccacc tgagcagctc 180 atggaaacaa atccaaacat gtggtgggag gatgttgtac aagcatatga acatttacgt 240 Sequence Listing (2) caactcggtt atgaagaaat agcagtggcc ggcctatcgt taggtggaat gatggcatta 300 aagttagcga cagagacaaa aataaaagct gttatatcga tgtgtgttcc tatgatattt 360 gatgataaaa ataatttaac agagtctttt gagcgttttg tacgacaata taaacagttt 420 gaaaaaaagg ataaagcgac gattgatcat gaaacagaac aaataatgga acaatccgtt 480 gatttgtttg aggaaattaa agcatttaat ttatcggtta aagaagtcat tgatttgatt 540 tatgcaccaa cacttgttat acaagcacgt aacgatgagg ttattaatcc tgaaagtgca 600 caatatatat atgattcagt tgaaacagat gaaaaagaaa taaagtggta tgaagaatca 660 ggccatgtta tcacattagg tgaagaacgc gaccaattac atgaagatat ttatatgttt 720 ctcgaacagt tagaatggga acaataa 747 <210> 140 <211> 248 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 140 Met Lys Ile Arg Gln Pro Gln Pro Phe Thr Phe Glu Ala Gly Lys Arg Ala Val Leu Leu Leu His Gly Phe Thr Gly His Ser Ala Asp Val Arg Met Leu Gly Arg Tyr Leu Glu Glu Lys Gly Tyr Thr Ser His Ala Pro Ile Tyr Arg Gly His Gly Leu Pro Pro Glu Gln Leu Met Glu Thr Asn Pro Asn Met Trp Trp Glu Asp Val Val Gln Ala Tyr Glu His Leu Arg Gln Leu Gly Tyr Glu Glu Ile Ala Val Ala Gly Leu Ser Leu Gly Gly Met Met Ala Leu Lys Leu Ala Thr Glu Thr Lys Ile Lys Ala Val Ile Sequence Listing (2) Ser Met Cys Val Pro Met Ile Phe Asp Asp Lys Asn Asn Leu Thr Glu Ser Phe Glu Arg Phe Val Arg Gln Tyr Lys Gln Phe Glu Lys Lys Asp Lys Ala Thr Ile Asp His Glu Thr Glu Gln Ile Met Glu Gln Ser Val Asp Leu Phe Glu Glu Ile Lys Ala Phe Asn Leu Ser Val Lys Glu Val Ile Asp Leu Ile Tyr Ala Pro Thr Leu Val Ile Gln Ala Arg Asn Asp Glu Val Ile Asn Pro Glu Ser Ala Gln Tyr Ile Tyr Asp Ser Val Glu Thr Asp Glu Lys Glu Ile Lys Trp Tyr Glu Glu Ser Gly His Val Ile Thr Leu Gly Glu Glu Arg Asp Gln Leu His Glu Asp Ile Tyr Met Phe Leu Glu Gln Leu Glu Trp Glu Gln <210> 141 <211> 699 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 141 atgcgtttgt ttttcctttt cggactgttt gccgtgctgt tgttggattc tatcaacgc:c 60 actgcatctc aagatcacca acagtctqag ctaacacccg aacatcaggt tcggatttta 120 gttcttggcg atagtatcag cgccgcctat ggccttgata ccgaacaggg ctgggtgac:g 180 Sequence Listing (2) ctgcttaagc agcgtgcaca acaacaatgc ggtcaaaaca gtgtaaatca agtgcttgtg 240 aataatgcgt cggtgagtgg ggagaccacg gccggtgggt tggcacggtt gcccatgctg 300 ttagatggaa gctctcatcc tgttcccaat attgtggtgc tggaactggg tggcaatgat 360 ggtcttcgtg gtttgtctcc cattgccatg aaacagaatt tattgagtat ggttgaacaa 420 agtcaggcgg tgaatgctga agtggttttg ttgggtatgt taatccctcc aaattatgga 480 gaagcgtatg tacagctttt ttccaatacc tttcaacagg tggccaatga aactggggta 540 gccttcaagc ctttcttctt ggaaggggtg gggggagtcc ctgaattaat gcaagcggat 600 ggtattcacc ccaccgcaga agctcagcct attttattgg acaatgcctg gccattaatt 660 gggccaatag ttttgaggaa atgtcgtgga atcacctga 699 <210> 142 <211> 232 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 142 Met Arg Leu Phe Phe Leu Phe Gly Leu Phe Ala Val Leu Leu Leu Asp Ser Ile Asn Ala Thr Ala Ser Gln Asp His Gln Gin Ser Glu Leu Thr Pro Glu His Gln Val Arg Ile Leu Val Leu Gly Asp Ser Ile Ser Ala Ala Tyr Gly Leu Asp Thr Glu Gln Gly Trp Val Thr Leu Leu Lys Gln Arg Ala Gln Gln Gln Cys Gly Gln Asn Ser Val Asn Gln Val Leu Val Asn Asn Ala Ser Val Ser Gly Glu Thr Thr Ala Gly Gly Leu Ala Arg Leu Pro Met Leu Leu Asp Gly Ser Ser His Pro Val Pro Asn Ile Val Sequence Listing (2) Val Leu Glu Leu Gly Gly Asn Asp Gly Leu Arg Gly Leu Ser Pro Ile Ala Met Lys Gln Asn Leu Leu Ser Met Val Glu Gln Ser Gln Ala Val Asn Ala Glu Val Val Leu Leu Gly Met Leu Ile Pro Pro Asn Tyr Gly Glu Ala Tyr Val Gln Leu Phe Ser Asn Thr Phe Gln Gln Val Ala Asn Glu Thr Gly Val Ala Phe Lys Pro Phe Phe Leu Glu Gly Val Gly Gly Val Pro Glu Leu Met Gln Ala Asp Gly Ile His Pro Thr Ala Glu Ala Gln Pro Ile Leu Leu Asp Asn Ala Trp Pro Leu Ile Gly Pro Ile Val Leu Arg Lys Cys Arg Gly Ile Thr <210> 143 <211> 660 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 143 atggcctggg agcaattcga acacggctgg cggcgcgcgc cgtcggccac gcccgcgac.a 60 gcgctggtgg tgctgttgca cggcgtgggc agcaatgcgc gcgatctgat gccgcttgc:g 120 gacatctgga gcgagagcct gccggacgtg gcgttcacct cgctcgacgg caccgaggc:c 180 tttgacggcg gcttcggcgg acgccaatgg ttcagcctgc gcgatgtcaa cgaaggcaat 240 cgcgaaaccc gtgtcgctgc cgcct.atccg gccttgcggc gcgtactcga ggcggaact.g 300 Sequence Listing (2) gcgtactggc agctttcttt cgatcgcctt gcgctggtcg gtttctcgca gggttcgatc 360 atggcgatgc atcacgtcgc gacgagcgcg gaaggcgctg ccgccgcggt cgcgtactcg 420 gggcggctcg cctcggtgat cgtcgcgcag aaccgcacgc cgctcacgct cgtgcacggc 480 gaggaagacg aagtgatccc ggtcgaggag ctggaatacg ccgcggacgc attcaacagc 540 gccggttacg cggtcgacgc ctacgcgttg cccggcattg gtcacacgat aaacgcggac 600 ggtgtcgagc tcggcaagca ggcgctggaa catgccttgg gtgctttgcc gcgcggttga 660 <210> 144 <211> 219 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 144 Met Ala Trp Glu Gln Phe Glu His Gly Trp Arg Arg Ala Pro Ser Ala Thr Pro Ala Arg Ala Leu Val Val Leu Leu His Gly Val Gly Ser Asn Ala Arg Asp Leu Met Pro Leu Ala Asp Ile Trp Ser Glu Ser Leu Pro Asp Val Ala Phe Thr Ser Leu Asp Gly Thr Glu Ala Phe Asp Gly Gly Phe Gly Gly Arg Gln Trp Phe Ser Leu Arg Asp Val Asn Glu Gly Asn Arg Glu Thr Arg Val Ala Ala Ala Tyr Pro Ala Leu Arg Arg Val Leu Glu Ala Glu Leu Ala Tyr Trp Gln Leu Ser Phe Asp Arg Leu Ala Leu Val Gly Phe Ser Gln Gly Ser Ile Met Ala Met His His Val Ala Thr Sequence Listing (2) Ser Ala Glu Gly Ala Ala Ala Ala Val Ala Tyr Ser Gly Arg Leu Ala Ser Val Ile Val Ala Gln Asn Arg Thr Pro Leu Thr Leu Val His Gly Glu Glu Asp Glu Val Ile Pro Val Glu Glu Leu Glu Tyr Ala Ala Asp Ala Phe Asn Ser Ala Gly Tyr Ala Val Asp Ala Tyr Ala Leu Pro Gly Ile Gly His Thr Ile Asn Ala Asp Gly Val Glu Leu Gly Lys Gln Ala Leu Glu His Ala Leu Gly Ala Leu Pro Arg Gly <210> 145 <211> 744 <212> DNA
<213> Bacillus thermoleovorans <400> 145 atgatgaaaa ttgttccgcc gaagccgttt ttctttgaag ccggggagcg ggcggtgctg 60 cttttgcatg ggtttaccgg caattccgcc gacgttcgga tgcttgggcg attcttggaa 120 tcgaaagggt atacgtgcca cgctccgatt tacaaagggc atggcgtgcc gccggaagag 180 ctcgtccaca ccggaccgga tgattggtgg caagacgtca tgaacggcta tcagtttttg 240 aaaaacaaag gctacgaaaa aattgccgtg gctggattgt cgcttggagg cgtattttct 300 ctcaaattag gctacactgt acctatacaa ggcattgtga cgatgtgcgc gccgatgtac 360 atcaaaagcg aagaaacgat gtacgaaggt gtgctcgagt atgcgcgcga gtataaaaag 420 cgggaaggga aatcagagga acaaatcgaa caggaaatgg aacggttcaa acaaacgcc.g 480 atgaagacgt tgaaagcctt gcaagaactc attgccgatg tgcgcgccca ccttgatttg 540 gtttatgcac cgacgttcgt cgtccaagcg cgccatgatg agatgatcaa tccagacagc 600 gcgaacatca tttataacga aattgaatcg ccggtcaaac aaatcaaatg gtatgagcaa 660 tcaggccatg tgattacgct tgatcaagaa aaagatcagc tgcatgaaga tatttatgca 720 Sequence Listing (2) tttcttgaat cgttagattg gtaa 744 <210> 146 <211> 247 <212> PRT
<213> Bacillus thermoleovorans <400> 146 Met Met Lys Ile Val Pro Pro Lys Pro Phe Phe Phe Glu Ala Gly Glu Arg Ala Val Leu Leu Leu His Gly Phe Thr Gly Asn Ser Ala Asp Val Arg Met Leu Gly Arg Phe Leu Glu Ser Lys Gly Tyr Thr Cys His Ala Pro Ile Tyr Lys Gly His Gly Val Pro Pro Glu Glu Leu Val His Thr Gly Pro Asp Asp Trp Trp Gln Asp Val Met Asn Gly Tyr Gln Phe Leu Lys Asn Lys Gly Tyr Glu Lys Ile Ala Val Ala Gly Leu Ser Leu Gly Gly Val Phe Ser Leu Lys Leu Gly Tyr Thr Val Pro Ile Gln Gly Ile Val Thr Met Cys Ala Pro Met Tyr Ile Lys Ser Glu Glu Thr Met Tyr Glu Gly Val Leu Glu Tyr Ala Arg Glu Tyr Lys Lys Arg Glu Gly Lys Ser Glu Glu Gln Ile Glu Gln Glu Met Glu Arg Phe Lys Gln Thr Pro Met Lys Thr Leu Lys Ala Leu Gln Glu Leu Ile Ala Asp Val Arg Ala Sequence Listing (2) His Leu Asp Leu Val Tyr Ala Pro Thr Phe Val Val Gln Ala Arg His Asp Glu Met Ile Asn Pro Asp Ser Ala Asn Ile Ile Tyr Asn Glu Ile Glu Ser Pro Val Lys Gln Ile Lys Trp Tyr Glu Gln Ser Gly His Val Ile Thr Leu Asp Gln Glu Lys Asp Gln Leu His Glu Asp Ile Tyr Ala Phe Leu Glu Ser Leu Asp Trp <210> 147 <211> 660 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 147 gtgccagacc taagtaacac ctccctgctg caattcaacg gctggacgct gcgggttcgg 60 caagcggctg ccagacctgc ccgtctgctg ctcatgctgc acggttacac cggcgacgaa 120 aactccatgt gggtgtttgc gaaggacatg ccgcctcatt actggattgc cgccccgcgc 180 gcgcctttcc ccgccgagcc gagaggctat tcctggcgtc cgcttcaccc ggcgaccttt 240 ggccgcccca gtctcgaaga acttgtgcct caggctgagg cgttgattcg ctttgtggac 300 gagtaccagg cttcggcggg gatcgaagcg tccgatttcg atgtgatggg attcagccag 360 ggcggagcga tgtccaacgt gctggcgttc ctgtatccgc agcgcatccg caaattgggg 420 atgctggcgg ggtttgtccc cggcgggttg gatgtgtatg ccaaaaatca tccgcttcaa 480 gggaagcggg tgttcgtcac gcatggcacg cgcgatgaca tggtgccgat tgaccgcgc:c 540 cgcgcctcgg ttgccgtgct ggagcaggca ggcgcgcaga ttgtttattg tgaggatgag 600 gttggtcaca aagtcagcct caattgcctg cgcgcgctgc gctcgtatct ggaagattag 660 <210> 148 Sequence Listing (2) <211> 219 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 148 Met Pro Asp Leu Ser Asn Thr Ser Leu Leu Gln Phe Asn Gly Trp Thr Leu Arg Val Arg Gln Ala Ala Ala Arg Pro Ala Arg Leu Leu Leu Met Leu His Gly Tyr Thr Gly Asp Glu Asn Ser Met Trp Val Phe Ala Lys Asp Met Pro Pro His Tyr Trp Ile Ala Ala Pro Arg Ala Pro Phe Pro Ala Glu Pro Arg Gly Tyr Ser Trp Arg Pro Leu His Pro Ala Thr Phe Gly Arg Pro Ser Leu Glu Glu Leu Val Pro Gln Ala Glu Ala Leu Ile Arg Phe Val Asp Glu Tyr Gln Ala Ser Ala Gly Ile Glu Ala Ser Asp Phe Asp Val Met Gly Phe Ser Gln Gly Gly Ala Met Ser Asn Val Leu Ala Phe Leu Tyr Pro Gln Arg Ile Arg Lys Leu Gly Met Leu Ala Gly Phe Val Pro Gly Gly Leu Asp Val Tyr Ala Lys Asn His Pro Leu Gln Gly Lys Arg Val Phe Val Thr His Gly Thr Arg Asp Asp Met Val Pro Ile Asp Arg Ala Arg Ala Ser Val Ala Val Leu Glu Gln Ala Gly Ala Sequence Listing (2) Gln Ile Val Tyr Cys Glu Asp Glu Val Gly His Lys Val Ser Leu Asn Cys Leu Arg Ala Leu Arg Ser Tyr Leu Glu Asp <210> 149 <211> 642 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 149 atgttacgca cactttacct gatcttggta ttgatggggc ttttcccatt gtcctctacg 60 gttatggccg atacgcccaa aacttccact attttagtgt ggggcgacag tttaagtgcg 120 gcttatggca tcccggtcga aaaaggctgg gtcaatttaa tgcgaaccaa gttaggcact 180 gagtttaatg tcgtcaatgg cagtattagt ggagaaacca ctgctggagg cttaagccgc 240 ttacctaaag cattaacaga ttatcaacct gattatgtat tgatagaatt aggggcgaat 300 gatggtttgc atggtattgc taccgacatc atgaaagcta accttagcaa aatgattgaa 360 ctcagtcaga ccaatcatgc caaggtggta ttaattggga ttcagctccc ccccaatttc 420 ggtaatgctt ttacggataa gtttgatgcc acttatacgg atttagctaa acaatataac 480 ttgcctttag tgccatcatt aatgaccaat gtggcagaaa attgggattt aattcaagct 540 gatggtttgc acccaaccgc agaagcacag ccattattgc ttgaaaatgt atggaaggtg 600 gtagaaaccg ttttaaaacc aactactccc gccaaatcat aa 642 <210> 150 <211> 213 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 150 Met Leu Arg Thr Leu Tyr Leu Ile Leu Val Leu Met Gly Leu Phe Pro Sequence Listing (2) Leu Ser Ser Thr Val Met Ala Asp Thr Pro Lys Thr Ser Thr Ile Leu Val Trp Gly Asp Ser Leu Ser Ala Ala Tyr Gly Ile Pro Val Glu Lys Gly Trp Val Asn Leu Met Arg Thr Lys Leu Gly Thr Glu Phe Asn Val Val Asn Gly Ser Ile Ser Gly Glu Thr Thr Ala Gly Gly Leu Ser Arg Leu Pro Lys Ala Leu Thr Asp Tyr Gln Pro Asp Tyr Val Leu Ile Glu Leu Gly Ala Asn Asp Gly Leu His Gly Ile Ala Thr Asp Ile Met Lys Ala Asn Leu Ser Lys Met Ile Glu Leu Ser Gln Thr Asn His Ala Lys Val Val Leu Ile Gly Ile Gln Leu Pro Pro Asn Phe Gly Asn Ala Phe Thr Asp Lys Phe Asp Ala Thr Tyr Thr Asp Leu Ala Lys Gln Tyr Asn Leu Pro Leu Val Pro Ser Leu Met Thr Asn Val Ala Glu Asn Trp Asp Leu Ile Gln Ala Asp Gly Leu His Pro Thr Ala Glu Ala Gln Pro Leu Leu Leu Glu Asn Val Trp Lys Val Val Glu Thr Val Leu Lys Pro Thr Thr Pro Ala Lys Ser Sequence Listing (2) <210> 151 <211> 1092 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 151 atgaccacat ctacacaaca catcagcgag ctacctctct taccaggccg tctcggcgac 60 cccgatcgcg tcttgaagac tgatccccgt gccgatcctc ggttggtcgc cgcctgcgcc 120 ccctttgctt tagacgttgc gcccccaccc gcaccggtga acgcgcactc gcccttgcag 180 gacaagctcg actacagtgc ggccaacgag gcgggcatgg aaaccgtgtt tgccgccctg 240 ttcgccgacc tcccgccgat gacgaacgtg gagcggcgga ccgaggtcat caagggcgtg 300 gacggtaacg acatcaagct gtatatccat acgccccagc acgtttctgg cccgctgccc 360 tgcgtgtatc atatacacgg cggcggcatg gtcattctga cggccgctgg ccctacctat 420 gtacgctggc gggacgagct tgccgccctg ggcatggtcg tggtgggtgt ggaattccgt 480 aatggcgccg gcaagcttgg caaccatccc tttccagccg ggctcaacga ctgcatgagt 540 ggcttacagt ggacgtttga ccacaaaacc accctgggga tctcgaagat aatcgtgtcc 600 ggtgagtctg gtggaggcaa tctctccctg gccgtgtgcc tcaaggccaa gaaggacaaa 660 cgccttgagc agattcatgg tgtctacgcc ttgtgtccgt acatttatgg cgcatgggcg 720 cagaagagca aagatctccc ttccctatac gaaaacgacg gctacttgat caactgtagc 780 ctgatggagg tgctggcaag cgtctatgac cctgaaggca aaaacgccac caatccgctg 840 tgctggccat actgggccac acgtgaggac ctgcaaggtc tgcctccgca tgtgatctcg 900 gtcaacgagt tagaccccct gcgagacgag gggctgaaat actatcagag gctcctggcg 960 gctggggtgc gtgtatacag ccggaccgtc aacggcacgt gccatgcggg cgacgtcctg 1020 ttccgcaaag cgctcccgga cgtgtacgca gccaccctcc gcgatatcaa ggggttcgca 1080 gactcgctgt ag 1092 <210> 152 <211> 363 <212> PRT
<213> Unknown Sequence Listing (2) <220>
<223> Obtained from an environmental sample <400> 152 Met Thr Thr Ser Thr Gln His Ile Ser Glu Leu Pro Leu Leu Pro Gly Arg Leu Gly Asp Pro Asp Arg Val Leu Lys Thr Asp Pro Arg Ala Asp Pro Arg Leu Val Ala Ala Cys Ala Pro Phe Ala Leu Asp Val Ala Pro Pro Pro Ala Pro Val Asn Ala His Ser Pro Leu Gln Asp Lys Leu Asp Tyr Ser Ala Ala Asn Glu Ala Gly Met Glu Thr Val Phe Ala Ala Leu Phe Ala Asp Leu Pro Pro Met Thr Asn Val Glu Arg Arg Thr Glu Val Ile Lys Gly Val Asp Gly Asn Asp Ile Lys Leu Tyr Ile His Thr Pro Gln His Val Ser Gly Pro Leu Pro Cys Val Tyr His Ile His Gly Gly Gly Met Val Ile Leu Thr Ala Ala Gly Pro Thr Tyr Val Arg Trp Arg Asp Glu Leu Ala Ala Leu Gly Met Val Val Val Gly Val Glu Phe Arg Asn Gly Ala Gly Lys Leu Gly Asn His Pro Phe Pro Ala Gly Leu Asn Asp Cys Met Ser Gly Leu Gln Trp Thr Phe Asp His Lys Thr Thr Leu Gly Ile Ser Lys Ile Ile Val Ser Gly Glu Ser Gly Gly Gly Asn Leu Sequence Listing (2) Ser Leu Ala Val Cys Leu Lys Ala Lys Lys Asp Lys Arg Leu Glu Gln Ile His Gly Val Tyr Ala Leu Cys Pro Tyr Ile Tyr Gly Ala Trp Ala Gln Lys Ser Lys Asp Leu Pro Ser Leu Tyr Glu Asn Asp Gly Tyr Leu Ile Asn Cys Ser Leu Met Glu Val Leu Ala Ser Val Tyr Asp Pro Glu Gly Lys Asn Ala Thr Asn Pro Leu Cys Trp Pro Tyr Trp Ala Thr Arg Glu Asp Leu Gln Gly Leu Pro Pro His Val Ile Ser Val Asn Glu Leu Asp Pro Leu Arg Asp Glu Gly Leu Lys Tyr Tyr Gln Arg Leu Leu Ala Ala Gly Val Arg Val Tyr Ser Arg Thr Val Asn Gly Thr Cys His Ala Gly Asp Val Leu Phe Arg Lys Ala Leu Pro Asp Val Tyr Ala Ala Thr Leu Arg Asp Ile Lys Gly Phe Ala Asp Ser Leu <210> 153 <211> 1194 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 153 atgaacaaac gaagtttgcg caagt.gtttg agcgctgtcg gtgtcgttgc gatcctgttc 60 Sequence Listing (2) tcggtgcaac aggtgctagc ctatgacgag atcgtgcaga agcaggcctt tgagcttgcc 120 tcctacacaa ccaccggcgg ggagacgatc aaggacgtcc gcatcggctg ggaggcctac 180 gggacgttga acgaggccaa ggacaacgtc atcctgatta cccacttcta ttccggcaac 240 agccatgccg ccggccgcta caccgccgag ggtcctgccg gctattggaa cgccatcatc 300 ggttcaggca agccgatcga taccgacaaa tactacgtcg tgtcgtccga caccctggtg 360 aatctcggcg ccaatgatcc caatgtcatc accaccggtc cggcctccat caatcccgac 420 accggcaagc cctacggcat gagctttccg atcgtgacga tgcgcgattt cgtcgaggtc 480 cagagggaat tgcttgatag cctcggcatc gagaggctgc atgcggtgat cggtgcctca 540 atgggcgccc tgcaggccta tgagtgggcc gcggcctatc ccgagcgggt cgagcgcctc 600 attccggtga tcggcagcgg ctgggccgac ggcgacctga tcggctggct caacatctgg 660 tcggcgccga tcaagctcga ccccaactgg aatggcggcg actactatga cggcgagccg 720 ccgctgcagg ggcttgccga gtcgctgaag atcgtgacgt tgcaggccaa ccactggaaa 780 tggtccaaca gcgtgttcgg ccgcgcctgg gccaaggacg gtgccgatcc ggcggcaagc 840 ttcgacaatc tgttcaggat cgaggcggtg cttgacgggg ccggccaggc ccgcgccaag 900 accagcgatg ccaatcattt cctctacctc gccaaggcca atcagctgtt ctatgccggc 960 cacggcgaaa ccctctatga aggcctgcgg gcgatcacgg cgccgacgct gatcatccat 1020 agcgatgacg acctgctgtt cccgcccgcc gcgatccgcg aaaccgccgc catgatcaga 1080 tcgggcggcg cgccggtcga ggtggtcggg ctgcacggcg ggcgcggtca tctggatgca 1140 gtcctgtcaa tcagccaggc cggcgcgcag atcggggcat tcctcgcgag gtga 1194 <210> 154 <211> 397 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 154 Met Asn Lys Arg Ser Leu Arg Lys Cys Leu Ser Ala Val Gly Val Val Ala Ile Leu Phe Ser Val Gln Gln Val Leu Ala Tyr Asp Glu Ile Val Sequence Listing (2) Gln Lys Gln Ala Phe Glu Leu Ala Ser Tyr Thr Thr Thr Gly Gly Glu Thr Ile Lys Asp Val Arg Ile Gly Trp Glu Ala Tyr Gly Thr Leu Asn Glu Ala Lys Asp Asn Val Ile Leu Ile Thr His Phe Tyr Ser Gly Asn Ser His Ala Ala Gly Arg Tyr Thr Ala Glu Gly Pro Ala Gly Tyr Trp Asn Ala Ile Ile Gly Ser Gly Lys Pro Ile Asp Thr Asp Lys Tyr Tyr Val Val Ser Ser Asp Thr Leu Val Asn Leu Gly Ala Asn Asp Pro Asn Val Ile Thr Thr Gly Pro Ala Ser Ile Asn Pro Asp Thr Gly Lys Pro Tyr Gly Met Ser Phe Pro Ile Val Thr Met Arg Asp Phe Val Glu Val Gln Arg Glu Leu Leu Asp Ser Leu Gly Ile Glu Arg Leu His Ala Val Ile Gly Ala Ser Met Gly Ala Leu Gln Ala Tyr Glu Trp Ala Ala Ala Tyr Pro Glu Arg Val Glu Arg Leu Ile Pro Val Ile Gly Ser Gly Trp Ala Asp Gly Asp Leu Ile Gly Trp Leu Asn Ile Trp Ser Ala Pro Ile Lys Leu Asp Pro Asn Trp Asn Gly Gly Asp Tyr Tyr Asp Gly Glu Pro Sequence Listing (2) Pro Leu Gln Gly Leu Ala Glu Ser Leu Lys Ile Val Thr Leu Gln Ala Asn His Trp Lys Trp Ser Asn Ser Val Phe Gly Arg Ala Trp Ala Lys Asp Gly Ala Asp Pro Ala Ala Ser Phe Asp Asn Leu Phe Arg Ile Glu Ala Val Leu Asp Gly Ala Gly Gln Ala Arg Ala Lys Thr Ser Asp Ala Asn His Phe Leu Tyr Leu Ala Lys Ala Asn Gln Leu Phe Tyr Ala Gly His Gly Glu Thr Leu Tyr Glu Gly Leu Arg Ala Ile Thr Ala Pro Thr Leu Ile Ile His Ser Asp Asp Asp Leu Leu Phe Pro Pro Ala Ala Ile Arg Glu Thr Ala Ala Met Ile Arg Ser Gly Gly Ala Pro Val Glu Val Val Gly Leu His Gly Gly Arg Gly His Leu Asp Gly Val Leu Ser Ile Ser Gln Ala Gly Ala Gln Ile Gly Ala Phe Leu Ala Arg <210> 155 <211> 747 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 155 atgaaaatta agttgccaga accattcaca ttcgaagcag gaaatcgagc agttttatt.a 60 ttacatggat ttactggcca ttcagcagat gtaagaatgc ttggtcgatt tctagaaaag 120 Sequence Listing (2) aaaggttata caactcatgc accaatttat agagggcatg gtcaagaacc agaagcatta 180 ttaaaatctt cacctgatga atggtgggag gatattttat cagcttataa tcatttgaaa 240 aatctaggat ataatgaaat tgctgttgct ggtctttcaa tgggtggtgc tttagcaatt 300 aaactagcta ctaagcatga aataaaaggt gtcattccaa tgtgtacacc aatgtacttt 360 gataatcaaa aacaattaac tcaagcattt ttaaattttg cacgacaatt taaaaaattt 420 gagaaaaaag atgaagaaac aattaaaaaa gaaatagatt tactacaaca aaattcagca 480 gaattattta atgagatcgg cacttttgta gaacaagtca atggtataat agatagagtt 540 gacgtaccta cattcgtagt tcaagcaagt aaagatgaaa taatcaatcc tgaaagtgcg 600 acatttattt atgaaaacat taaaaatgaa aaaaaagatt taaaatggta taaaaattca 660 actcacttga taacatttgg cgatgaaaaa gatgtcttac atgaagatat atatcatttt 720 ttagaaacat tagattggaa caactaa 747 <210> 156 <211> 248 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 156 Met Lys Ile Lys Leu Pro Glu Pro Phe Thr Phe Glu Ala Gly Asn Arg Ala Val Leu Leu Leu His Gly Phe Thr Gly His Ser Ala Asp Val Arg Met Leu Gly Arg Phe Leu Glu Lys Lys Gly Tyr Thr Thr His Ala Pro Ile Tyr Arg Gly His Gly Gln Glu Pro Glu Ala Leu Leu Lys Ser Ser Pro Asp Glu Trp Trp Glu Asp Ile Leu Ser Ala Tyr Asn His Leu Lys Asn Leu Gly Tyr Asn Glu Ile Ala Val Ala Gly Leu Ser Met Gly Gly Sequence Listing (2) Ala Leu Ala Ile Lys Leu Ala Thr Lys His Glu Ile Lys Gly Val Ile Pro Met Cys Thr Pro Met Tyr Phe Asp Asn Gln Lys Gln Leu Thr Gln Ala Phe Leu Asn Phe Ala Arg Gln Phe Lys Lys Phe Glu Lys Lys Asp Glu Glu Thr Ile Lys Lys Glu Ile Asp Leu Leu Gln Gln Asn Ser Ala Glu Leu Phe Asn Glu Ile Gly Thr Phe Val Glu Gln Val Asn Gly Ile Ile Asp Arg Val Asp Val Pro Thr Phe Val Val Gln Ala Ser Lys Asp Glu Ile Ile Asn Pro Glu Ser Ala Thr Phe Ile Tyr Glu Asn Ile Lys Asn Glu Lys Lys Asp Leu Lys Trp Tyr Lys Asn Ser Thr His Leu Ile Thr Phe Gly Asp Glu Lys Asp Val Leu His Glu Asp Ile Tyr His Phe Leu Glu Thr Leu Asp Trp Asn Asn <210> 157 <211> 1350 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 157 atggcaacaa tgatgcgcgg agcaagcaag ctcctggctg gcatggccct ggcggtatcg 60 Sequence Listing (2) gctttgactg caaccggcga agcgttcgcc gcgaacaatc tgccgattgt attggtacac 120 ggctttatcg gcttcggccc gaacgagctg caaggcaccg gtttcaaata ctggggcggt 180 ttcaacgatg tcgaggctta cctcagaacc aataacggca gccatcaggt tcataccgcc 240 gcagtgggcc cggtcagctc gaactgggat cgtgcggtcg agctgttcta tcagatcaag 300 ggcggctgtg ccgattacgg cgctgcacgc accgccagat ttgctgccta cggcaaaatc 360 cagaagccgg ccggcaaatg ctgggcgacc gacccgaaca acaacccgaa caactacccg 420 ttggcactgt acccgcaatg ggatgccgcc cacccgatcc atctggtgtc gcacagccag 480 ggcggtcaga ccatccgcac gatgatccag ctgctggaaa acggctcgcc ttacggcaat 540 gaaggcaacg gcgcactgta taccggcggc aagatcggct gggtgaaaag cgccaccac:c 600 atttccaccc cgcataacgg caccacgctg cgcgacgtga tcatcgacta cgtaccgaaa 660 atttccgaac tggccggcaa agtcgtcgaa gtggccggac tcggcggcag cggcggccgg 720 atctacaact tcaagctgga gcaatacggc ctggcacaag gcccgtcgga aaacttcca.g 780 gatttcatca accgcaccaa gggcgcaccg tactggaaac tggacaacaa agactcggca 840 cagtgggatc tgggcccgga tggcgcgaaa gagctgaaca gctgggtcaa gacctcgcc:g 900 aacgtgtatt actactcgat cgccaaccat gcaaccgaac agggcagctt ctgctgcaac 960 gaaaccgacc gcctgatcgc cccgatccag agcagcaatt accagtatgc ccgcaacgac 1020 atgatcttct tcctgaagaa caccgccggc gaatgggtgg tcccgtccat cctcaaccgc 1080 ggcatgggtt catacaccca gaccgatccg agccgggtac tgatcgacag caactggttc 1140 ctcaacgacg gcgtggtcaa ctacatcagc atgaagtcgc cgtcgggcca gccggtacc[c 1200 aactacaacg gcacctcggt caagggttac tggaatcacc tcggctacta caacaactac 1260 gatcacttcg acgtcatcgg ctggctgaac tccagctcga ccgtttaccc gatctacaac 1320 tacctgaccg gtatcctgta cggtctgtga 1350 <210> 158 <211> 449 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 158 Sequence Listing (2) Met Ala Thr Met Met Arg Gly Ala Ser Lys Leu Leu Ala Gly Met Ala Leu Ala Val Ser Ala Leu Thr Ala Thr Gly Glu Ala Phe Ala Ala Asn Asn Leu Pro Ile Val Leu Val His Gly Phe Ile Gly Phe Gly Pro Asn Glu Leu Gln Gly Thr Gly Phe Lys Tyr Trp Gly Gly Phe Asn Asp Val Glu Ala Tyr Leu Arg Thr Asn Asn Gly Ser His Gln Val His Thr Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu Leu Phe Tyr Gln Ile Lys Gly Gly Cys Ala Asp Tyr Gly Ala Ala Arg Thr Ala Arg Phe Ala Ala Tyr Gly Lys Ile Gln Lys Pro Ala Gly Lys Cys Trp Ala Thr Asp Pro Asn Asn Asn Pro Asn Asn Tyr Pro Leu Ala Leu Tyr Pro Gln Trp Asp Ala Ala His Pro Ile His Leu Val Ser His Ser Gln Gly Gly Gln Thr Ile Arg Thr Met Ile Gln Leu Leu Glu Asn Gly Ser Pro Tyr Gly Asn Glu Gly Asn Gly Ala Leu Tyr Thr Gly Gly Lys Ile Gly Trp Val Lys Ser Ala Thr Thr Ile Ser Thr Pro His Asn Gly Thr Thr Leu Arg Asp Val Ile Ile Asp Tyr Val Pro Lys Ile Ser Glu Leu Sequence Listing (2) Ala Gly Lys Val Val Glu Val Ala Gly Leu Gly Gly Ser Gly Gly Arg Ile Tyr Asn Phe Lys Leu Glu Gln Tyr Gly Leu Ala Gln Gly Pro Ser Glu Asn Phe Gln Asp Phe Ile Asn Arg Thr Lys Gly Ala Pro Tyr Trp Lys Leu Asp Asn Lys Asp Ser Ala Gln Trp Asp Leu Gly Pro Asp Gly Ala Lys Glu Leu Asn Ser Trp Val Lys Thr Ser Pro Asn Val Tyr Tyr Tyr Ser Ile Ala Asn His Ala Thr Glu Gln Gly Ser Phe Cys Cys Asn Glu Thr Asp Arg Leu Ile Ala Pro Ile Gln Ser Ser Asn Tyr Gln Tyr Ala Arg Asn Asp Met Ile Phe Phe Leu Lys Asn Thr Ala Gly Glu Trp Val Val Pro Ser Ile Leu Asn Arg Gly Met Gly Ser Tyr Thr Gln Thr Asp Pro Ser Arg Val Leu Ile Asp Ser Asn Trp Phe Leu Asn Asp Gly Val Val Asn Tyr Ile Ser Met Lys Ser Pro Ser Gly Gln Pro Val Arg Asn Tyr Asn Gly Thr Ser Val Lys Gly Tyr Trp Asn His Leu Gly Tyr Tyr Asn Asn Tyr Asp His Phe Asp Val Ile Gly Trp Leu Asn Ser Ser Sequence Listing (2) Ser Thr Val Tyr Pro Ile Tyr Asn Tyr Leu Thr Gly Ile Leu Tyr Gly Leu <210> 159 <211> 906 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 159 atgagcacgg tcggggccga ggaggcacgc atccggttcc gctccttcac ggtcgacctc 60 cgcgatccag cgtcgctgcc ggcggtcggg gaggtcaccg agctgagcgt cgacggcgcc 120 gagggccccc gccccgcccg gctctaccgg ccgaccgacg ctggtgccga cacgcggttg 180 ccactggtgg tgttcttcca cgggggcggc ttcgtgatcg gcgacctcga cacccacgag 240 ggccaggcac gccgggtgtg ccgcgagctc ggcgcgctcg tgctcagcgt cgactaccgc 300 ctcgcacccg agcatcccta cccggccgcc cgcgaggact gcctcgccgc gacccgctgg 360 gccgccgacc acgccgacga cctcggcgcc gaccccgacc ggctggtggt cgcgggagac 420 agcgccggcg gcaacctcgc cgccgtggtc gcccaggact gccgcgacgg gcagggcccg 480 gcactggccg cccagctcct gatctatccg gccaccgaca tgaccgatcc gggcggggag 540 gtctatccct cccgccagga gaacgccgag ggctacttcc tcaccggcga cgacatgcgc 600 tggttcgaga gcagctatct cggcggtgcg acggccgggg agcccggcaa ctccccgatc 660 cacggggacc tgaccggtct tcccccggcc gtggtcgtca ccgcggagtt cgatccgctg 720 cgcgacgagg gcgaggccta cgcgcgggcg ctcgaggggg ccggcgtgcc ggtcgtgtcg 780 atccgcttcg acgggctcat ccacggcttc ttcgacatgg gaccgatctc gcccgcgtgt 840 gaccgggcgg ccgacgagac ctgccgggcc ctgtcggacc tgctgcagcc cgcggcgtcg 900 ggatga 906 <210> 160 <211> 301 <212> PRT

Sequence Listing (2) <213> Unknown <220>
<223> Obtained from an environmental sample <400> 160 Met Ser Thr Val Gly Ala Glu Glu Ala Arg Ile Arg Phe Arg Ser Phe Thr Val Asp Leu Arg Asp Pro Ala Ser Leu Pro Ala Val Gly Glu Val Thr Glu Leu Ser Val Asp Gly Ala Glu Gly Pro Arg Pro Ala Arg Leu Tyr Arg Pro Thr Asp Ala Gly Ala Asp Thr Arg Leu Pro Leu Val Val Phe Phe His Gly Gly Gly Phe Val Ile Gly Asp Leu Asp Thr His Glu Gly Gln Ala Arg Arg Val Cys Arg Glu Leu Gly Ala Leu Val Leu Ser Val Asp Tyr Arg Leu Ala Pro Glu His Pro Tyr Pro Ala Ala Arg Glu Asp Cys Leu Ala Ala Thr Arg Trp Ala Ala Asp His Ala Asp Asp Leu Gly Ala Asp Pro Asp Arg Leu Val Val Ala Gly Asp Ser Ala Gly Gly Asn Leu Ala Ala Val Val Ala Gln Asp Cys Arg Asp Gly Gln Gly Pro Ala Leu Ala Ala Gln Leu Leu Ile Tyr Pro Ala Thr Asp Met Thr Asp Pro Gly Gly Glu Val Tyr Pro Ser Arg Gln Glu Asn Ala Glu Gly Tyr Sequence Listing (2) Phe Leu Thr Gly Asp Asp Met Arg Trp Phe Glu Ser Ser Tyr Leu Gly Gly Ala Thr Ala Gly Glu Pro Gly Asn Ser Pro Ile His Gly Asp Leu Thr Gly Leu Pro Pro Ala Val Val Val Thr Ala Glu Phe Asp Pro Leu Arg Asp Glu Gly Glu Ala Tyr Ala Arg Ala Leu Glu Gly Ala Gly Val Pro Val Val Ser Ile Arg Phe Asp Gly Leu Ile His Gly Phe Phe Asp Met Gly Pro Ile Ser Pro Ala Cys Asp Arg Ala Ala Asp Glu Thr Cys Arg Ala Leu Ser Asp Leu Leu Gln Pro Ala Ala Ser Gly <210> 161 <211> 990 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 161 atgcaattct ggcaacgact gcaagccctt ttcaccccgc ggggtcgctc cgcccacccg 60 gaacaggaca ccgcatcgac gtcggcgaac acacacacgt cggcaaaagg ggcctccgtt 120 cccgaacccc aaacgctgcc ccatccacca cgaatcgatt gggtgaactg catcaatacc 180 gctggaaccc accgcatgat ctactacgaa tggggcgcgc ccgacaatcc tgaagtcgtc 240 ttttgcgttc acggcttgac gcgaaccggg cgtgatttcg acgcgcttgc ggctcatttg 300 agccgccgct atcgggtgat ctgtcccgac gtcgtcgggc gcggactttc ggattggctt 360 cccaacaaga gcgaatacgc gatcccacgc tacgcgagcg atctcacgac gctcattcag 420 aaaatccgac cccagaaact ctactgggtg ggtacctcca tgggcgggtt gatcgcaatg 480 Sequence Listing (2) gcaatcgctg cgcaacccac ttcaccgatc gcaaaactgg tgttgaacga tgttggcgcc 540 gtgctcagtc aggcgtcgtt acaacggatc ggcgcgtacg tcggtgccga gccgcaattt 600 gccacgttcg acgatgccgt tcagtacgta cgtactgtta gcgccccgtt cggcccactc 660 agcgacgcac aatggcgtca tctgacgtgg cacgccgtcc gacaagaact ggacggacgt 720 tgggtgttgc gctacgaccc aggcatcgct gttccgtttc gcatcgcgtt tcctgcccaa 780 gatgtggcct tgtgggagct ctacgacgca atacgctgcc cgacgctcct tttgcgtggc 840 gccgaatcgg acctcttgac gcgggaggtg gccgaagcga tgacgcgccg cggcccgcgg 900 gcgcgactcg tcgaaattcc cggtgtgggt cacgcaccgg cgttgctcga tcgcgcgcag 960 atcgacgaaa tcagcgcatt tctcgattag 990 <210> 162 <211> 329 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 162 Met Gln Phe Trp Gln Arg Leu Gln Ala Leu Phe Thr Pro Arg Gly Arg Ser Ala His Pro Glu Gln Asp Thr Ala Ser Thr Ser Ala Asn Thr His Thr Ser Ala Lys Gly Ala Ser Val Pro Glu Pro Gln Thr Leu Pro His Pro Pro Arg Ile Asp Trp Val Asn Cys Ile Asn Thr Ala Gly Thr His Arg Met Ile Tyr Tyr Glu Trp Gly Ala Pro Asp Asn Pro Glu Val Val Phe Cys Val His Gly Leu Thr Arg Thr Gly Arg Asp Phe Asp Ala Leu Ala Ala His Leu Ser Arg Arg Tyr Arg Val Ile Cys Pro Asp Val Val Sequence Listing (2) Gly Arg Gly Leu Ser Asp Trp Leu Pro Asn Lys Ser Glu Tyr Ala Ile Pro Arg Tyr Ala Ser Asp Leu Thr Thr Leu Ile Gln Lys Ile Arg Pro Gln Lys Leu Tyr Trp Val Gly Thr Ser Met Gly Gly Leu Ile Ala Met Ala Ile Ala Ala Gln Pro Thr Ser Pro Ile Ala Lys Leu Val Leu Asn Asp Val Gly Ala Val Leu Ser Gln Ala Ser Leu Gln Arg Ile Gly Ala Tyr Val Gly Ala Glu Pro Gln Phe Ala Thr Phe Asp Asp Ala Val Gln Tyr Val Arg Thr Val Ser Ala Pro Phe Gly Pro Leu Ser Asp Ala Gln Trp Arg His Leu Thr Trp His Ala Val Arg Gln Glu Leu Asp Gly Arg Trp Val Leu Arg Tyr Asp Pro Gly Ile Ala Val Pro Phe Arg Ile Ala Phe Pro Ala Gin Asp Val Ala Leu Trp Glu Leu Tyr Asp Ala Ile Arg Cys Pro Thr Leu Leu Leu Arg Gly Ala Glu Ser Asp Leu Leu Thr Arg Glu Val Ala Glu Ala Met Thr Arg Arg Gly Pro Arg Ala Arg Leu Val Glu Ile Pro Gly Val Gly His Ala Pro Ala Leu Leu Asp Arg Ala Gln Sequence Listing (2) Ile Asp Glu Ile Ser Ala Phe Leu Asp <210> 163 <211> 747 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 163 atgaaaatta agttgccaga accattcaca ttcgaagcag gaaatcgagc agttttatta 60 ttacatggat ttactggcca ttcagcagat gtaagaatgc ttggtcgatt tctagaaaag 120 aaaggttata caactcatgc accaatttat agagggcatg gtcaagagcc agaagcatta 180 ttaaaatctt cacctgatga atggtgggag gatattttat cagcttataa tcatttgaga 240 aatctaggat ataatgaaat tgctgttgct ggtctttcaa tgggtggtgc tttagcaatt 300 aaactagcta ccaagcatga aataaaaggt gtcattccaa tgtgtacacc aatgtacttt 360 gataatcaaa aacaattaac taaagcattt ttaaactttg cacgacaatt taaaaaattt 420 gagaaaaaag atgaacaaac aattaaaaaa gaaatagatt tactacaaca aaattcagca 480 gaattattta atgaaatcgg cacttttgta gaacaagtca atggtataat agatagagtt 540 gacgtaccta cattcgtagt tcaagcaagt aaagatgaaa taataaatcc tgaaagtgcg 600 acatttattt atgaaaacat taaaaatgaa aaaaaagatt taaaatggta taaaaactca 660 actcacttga taacatttgg cgatgaaaaa gatgttttac atgaagatat atatcatttt 720 ttagaaacat tagattggaa caactaa 747 <210> 164 <211> 248 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 164 Met Lys Ile Lys Leu Pro Glu Pro Phe Thr Phe Glu Ala Gly Asn Arg Sequence Listing (2) Ala Val Leu Leu Leu His Gly Phe Thr Gly His Ser Ala Asp Val Arg Met Leu Gly Arg Phe Leu Glu Lys Lys Gly Tyr Thr Thr His Ala Pro Ile Tyr Arg Gly His Gly Gln Glu Pro Glu Ala Leu Leu Lys Ser Ser Pro Asp Glu Trp Trp Glu Asp Ile Leu Ser Ala Tyr Asn His Leu Arg Asn Leu Gly Tyr Asn Glu Ile Ala Val Ala Gly Leu Ser Met Gly Gly Ala Leu Ala Ile Lys Leu Ala Thr Lys His Glu Ile Lys Gly Val Ile Pro Met Cys Thr Pro Met Tyr Phe Asp Asn Gln Lys Gln Leu Thr Lys Ala Phe Leu Asn Phe Ala Arg Gln Phe Lys Lys Phe Glu Lys Lys Asp Glu Gln Thr Ile Lys Lys Glu Ile Asp Leu Leu Gln Gln Asn Ser Ala Glu Leu Phe Asn Glu Ile Gly Thr Phe Val Glu Gln Val Asn Gly Ile Ile Asp Arg Val Asp Val Pro Thr Phe Val Val Gln Ala Ser Lys Asp Glu Ile Ile Asn Pro Glu Ser Ala Thr Phe Ile Tyr Glu Asn Ile Lys Asn Glu Lys Lys Asp Leu Lys Trp Tyr Lys Asn Ser Thr His Leu Ile Thr Phe Gly Asp Glu Lys Asp Val Leu His Glu Asp Ile Tyr His Phe Sequence Listing (2) Leu Glu Thr Leu Asp Trp Asn Asn <210> 165 <211> 918 <212> DNA
<213> Sulfolobus solfataricus Pl <400> 165 atgcccctag atcctagaat taaaaagtta ctagaatcag ctcttactat accaattggt 60 aaagccccag tagaagaggt aagaaagata tttaggcaat tagcgtcggc agctcccaaa 120 gtcgaagttg gaaaagtaga agatataaaa ataccaggca gtgaaaccgt tataaacgct 180 agagtgtatt ttccgaagag tagcggtcct tatggtgttc tagtgtatct tcatggaggc 240 ggttttgtaa taggcgatgt ggaatcttat gacccattat gtagagcaat tacaaatgcg 300 tgcaattgcg ttgtagtatc agtggactat aggttagctc cagaatacaa gtttccttct 360 gcagttatcg attcatttga cgctactaat tgggtttata acaatttaga taaatttgat 420 ggaaagatgg gagttgctat tgcgggagat agtgctggag gaaatttggc agcggttgta 480 gctcttcttt caaagggtaa aattaatttg aagtatcaaa tactggttta cccagcggta 540 agtttagata acgtttcaag atccatgata gagtactctg atgggttctt ccttaccaga 600 gagcatatag agtggttcgg ttctcaatac ttacgaagcc ctgcagattt gctagacttt 660 aggttctctc caattctggc gcaagatttc aacggattac ctccagcctt gataataaca 720 gcagaatacg atccactaag ggatcaagga gaagcgtatg caaataaact actacaagct 780 ggagtctcag ttactagtgt gagatttaac aacgttatac acggattcct ctcattcttt 840 ccgttgatgg agcaaggaag agatgctata ggtctgatag ggtctgtgtt aagacgagta 900 ttttatgata aaatttaa 918 <210> 166 <211> 305 <212> PRT
<213> Sulfolobus solfataricus P1 <400> 166 Met Pro Leu Asp Pro Arg Ile Lys Lys Leu Leu Glu Ser Ala Leu Thr Sequence Listing (2) Ile Pro Ile Gly Lys Ala Pro Val Glu Glu Val Arg Lys Ile Phe Arg Gln Leu Ala Ser Ala Ala Pro Lys Val Glu Val Gly Lys Val Glu Asp Ile Lys Ile Pro Gly Ser Glu Thr Val Ile Asn Ala Arg Val Tyr Phe Pro Lys Ser Ser Gly Pro Tyr Gly Val Leu Val Tyr Leu His Gly Gly Gly Phe Val Ile Gly Asp Val Glu Ser Tyr Asp Pro Leu Cys Arg Ala Ile Thr Asn Ala Cys Asn Cys Val Val Val Ser Val Asp Tyr Arg Leu Ala Pro Glu Tyr Lys Phe Pro Ser Ala Val Ile Asp Ser Phe Asp Ala Thr Asn Trp Val Tyr Asn Asn Leu Asp Lys Phe Asp Gly Lys Met Gly Val Ala Ile Ala Gly Asp Ser Ala Gly Gly Asn Leu Ala Ala Val Val Ala Leu Leu Ser Lys Gly Lys Ile Asn Leu Lys Tyr Gln Ile Leu Val Tyr Pro Ala Val Ser Leu Asp Asn Val Ser Arg Ser Met Ile Glu Tyr Ser Asp Gly Phe Phe Leu Thr Arg Glu His Ile Glu Trp Phe Gly Ser Gln Tyr Leu Arg Ser Pro Ala Asp Leu Leu Asp Phe Arg Phe Ser Pro Sequence Listing (2) Ile Leu Ala Gln Asp Phe Asn Gly Leu Pro Pro Ala Leu Ile Ile Thr Ala Glu Tyr Asp Pro Leu Arg Asp Gln Gly Glu Ala Tyr Ala Asn Lys Leu Leu Gln Ala Gly Val Ser Val Thr Ser Val Arg Phe Asn Asn Val Ile His Gly Phe Leu Ser Phe Phe Pro Leu Met Glu Gln Gly Arg Asp Ala Ile Gly Leu Ile Gly Ser Val Leu Arg Arg Val Phe Tyr Asp Lys Ile <210> 167 <211> 927 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 167 atgtcacttg atccacaaac gaaatttgta ttagcccaat tggctgctgc cgatgctcct 60 ccaatggaga ctttgtcacc ggaaatggct agacaggcat tcatattgcc gcaaggtgc:a 120 gtggaggaag tagggaaggt agaaaatcga acgattcctg gtcctgcaac ggacatccc:t 180 gtccgtgttt attaccctaa agaactccag cctgaaaatc ccgcgctagt tttctatca.t 240 ggcggcggct gggtaattgg caatttggac tctcatgacg atatttgccg tgctttaacc 300 aacctcgcca actgcgtgac cattt.ccgtc gactaccgcc tggctccaga gaataaatt.t 360 cctgctgcgg ttgaggatgc ttatgctgct gcacaatatg tgtatgacca tgcagaagE,~.t 420 ttcaaagtcg acaagacccg tattgccgtt ggcggtgaca gtgccggtgg aaatcttgcc 480 gctgtcgtga cgaatctggc aaaagacaaa aactcacctt ctatttgttt ccaacttctg 540 atttatccaa gcaccaatgc aggtggtgag ccaacagcat caatggttga gaatgcccat 600 Sequence Listing (2) ggctatttct tagaaaaagg cacgatggat tggttccgtg actgctacct gaacagtgaa 660 gaagacaaac agaatccgct agtctcaccg atgctttatg atgatttcca aggactgcct 720 ccagcaattg tgattactgc cgagtacgat ccattgcgag atgaaggcga agcatatgcc 780 aaaaagctgg gcgaagcagg ggttgctgtc caaaccatcc gctttgacgg tacgattcat 840 ggctttgtca gcatgtcggc tgtcattagc caagggaagg cggcattgga aaaagcagca 900 gaggctttaa ctaaagcctt tcaataa 927 <210> 168 <211> 308 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 168 Met Ser Leu Asp Pro Gln Thr Lys Phe Val Leu Ala Gln Leu Ala Ala Ala Asp Ala Pro Pro Met Glu Thr Leu Ser Pro Glu Met Ala Arg Gln Ala Phe Ile Leu Pro Gln Gly Ala Val Glu Glu Val Gly Lys Val Glu Asn Arg Thr Ile Pro Gly Pro Ala Thr Asp Ile Pro Val Arg Val Tyr Tyr Pro Lys Glu Leu Gln Pro Glu Asn Pro Ala Leu Val Phe Tyr His Gly Gly Gly Trp Val Ile Gly Asn Leu Asp Ser His Asp Asp Ile Cys Arg Ala Leu Thr Asn Leu Ala Asn Cys Val Thr Ile Ser Val Asp Tyr Arg Leu Ala Pro Glu Asn Lys Phe Pro Ala Ala Val Glu Asp Ala Tyr Sequence Listing (2) Ala Ala Ala Gln Tyr Val Tyr Asp His Ala Glu Asp Phe Lys Val Asp Lys Thr Arg Ile Ala Val Gly Gly Asp Ser Ala Gly Gly Asn Leu Ala Ala Val Val Thr Asn Leu Ala Lys Asp Lys Asn Ser Pro Ser Ile Cys Phe Gln Leu Leu Ile Tyr Pro Ser Thr Asn Ala Gly Gly Glu Pro Thr Ala Ser Met Val Glu Asn Ala His Gly Tyr Phe Leu Glu Lys Gly Thr Met Asp Trp Phe Arg Asp Cys Tyr Leu Asn Ser Glu Glu Asp Lys Gln Asn Pro Leu Val Ser Pro Met Leu Tyr Asp Asp Phe Gln Gly Leu Pro Pro Ala Ile Val Ile Thr Ala Glu Tyr Asp Pro Leu Arg Asp Glu Gly Glu Ala Tyr Ala Lys Lys Leu Gly Glu Ala Gly Val Ala Val Gln Thr Ile Arg Phe Asp Gly Thr Ile His Gly Phe Val Ser Met Ser Ala Val Ile Ser Gln Gly Lys Ala Ala Leu Glu Lys Ala Gly Glu Ala Leu Thr Lys Ala Phe Gln <210> 169 <211> 789 <212> DNA
<213> Thermococcus CL-2 Sequence Listing (2) <400> 169 atggaggttt acaaggccaa attcggcgaa gcaaagctcg gctgggtcgt tctggttcat 60 ggcctcggcg agcacagcgg aaggtatgga agactgatta aggaactcaa ctatgccggc 120 tttggagttt acaccttcga ctggcccggc cacgggaaga gcccgggcaa gagagggcac 180 acgagcgtcg aggaggcgat ggaaatcatc gactcgataa tcgaggagat cagggagaag 240 cccttcctct tcggccacag cctcggtggt ctaactgtca tcaggtacgc tgagacgcgg 300 cccgataaaa tacggggatt aatagcttcc tcgcctgccc tcgccaagag cccggaaacg 360 ccgggcttca tggtggccct cgcgaagttc cttggaaaga tcgccccggg agttgttctc 420 tccaacggca taaagccgga actcctctcg aggaacaggg acgccgtgag gaggtacgtt 480 gaagacccac tcgtccacga caggatttcg gccaagctgg gaaggagcat cttcgtgaac 540 atggagctgg cccacaggga ggcggacaag ataaaagtcc cgatcctcct tctgatcggc 600 actggcgatg taataacccc gcctgaaggc tcacgcagac tcttcgagga gctggccgtc 660 gagaacaaaa ccctgaggga gttcgagggg gcgtaccacg agatatttga agaccccgag 720 tgggccgagg agttccacga aacaattgtt aagtggctgg ttgaaaaatc gtactcttc:g 780 gctcaataa 789 <210> 170 <211> 262 <212> PRT
<213> Thermococcus CL-2 <400> 170 Met Glu Val Tyr Lys Ala Lys Phe Gly Glu Ala Lys Leu Gly Trp Val Val Leu Val His Gly Leu Gly Glu His Ser Gly Arg Tyr Gly Arg Leu Ile Lys Glu Leu Asn Tyr Ala Gly Phe Gly Val Tyr Thr Phe Asp Trp Pro Gly His Gly Lys Ser Pro Gly Lys Arg Gly His Thr Ser Val Glu Glu Ala Met Glu Ile Ile Asp Ser Ile Ile Glu Glu Ile Arg Glu Lys Sequence Listing (2) Pro Phe Leu Phe Gly His Ser Leu Gly Gly Leu Thr Val Ile Arg Tyr Ala Glu Thr Arg Pro Asp Lys Ile Arg Gly Leu Ile Ala Ser Ser Pro Ala Leu Ala Lys Ser Pro Glu Thr Pro Gly Phe Met Val Ala Leu Ala Lys Phe Leu Gly Lys Ile Ala Pro Gly Val Val Leu Ser Asn Gly Ile Lys Pro Glu Leu Leu Ser Arg Asn Arg Asp Ala Val Arg Arg Tyr Val Glu Asp Pro Leu Val His Asp Arg Ile Ser Ala Lys Leu Gly Arg Ser Ile Phe Val Asn Met Glu Leu Ala His Arg Glu Ala Asp Lys Ile Lys Val Pro Ile Leu Leu Leu Ile Gly Thr Gly Asp Val Ile Thr Pro Pro Glu Gly Ser Arg Arg Leu Phe Glu Glu Leu Ala Val Glu Asn Lys Thr Leu Arg Glu Phe Glu Gly Ala Tyr His Glu Ile Phe Glu Asp Pro Glu Trp Ala Glu Glu Phe His Glu Thr Ile Val Lys Trp Leu Val Glu Lys Ser Tyr Ser Ser Ala Gln <210> 171 <211> 891 <212> DNA

Sequence Listing (2) <213> Unknown <220>
<223> Obtained from an environmental sample <400> 171 atggcaagtg aagcactgac gatgatcgtg aacttgttgc gcagccagcg cccgttgcag 60 gaaccgacgg tggaacaaat gcgtgcgggg ttggaggcga tggcgcaaat gtcgccgcta 120 cctgcggacg tggaattgac caccgtggac gcaggaggtg tgcctggtgc ctgggtgcgc 180 gtgcccgaga gcgaccccga ccgcgtcgtg ctgtaccttc atggaggagg gtacgtcatt 240 gggtcgattc ggacacatcg cgaccttgct cagcgaattg cccgcgctgc ccgttgccgt 300 gtgttgttga tcgactaccg gctcgcaccc gaacacccgc atccggccgc ggtggaagac 360 tcgacgcgag cgtaccgatg gctcctcgag accggaagcg cccccaaacg catggccatt 420 gccggcgatt cggccggcgg cgggctgacg gtggcgacac tggtcgcact gcgagacgcg 480 ggagtgcccc tgcccgctgc cgccgtatgc ttatcgcctt gggtcgactt ggagggaatc 540 ggcgagtcga tgaccacgaa ggccgcagtc gatccgatgg tccaacgaga gccgttgctc 600 cgcatggcgt cgatgtacct ggcgggtcag gacccgcgca cgcctctggc ggctccgttg 660 tacgccgact tgcgagagct gccgcccttg ttgattcagg tgggtactgc ggaaaccctt 720 ctcgacgact cgatgcgcct ggctgagcgc gcacgcgctg cgggcgtaca ggtgacgctc 780 gaaccttggg aagacatgat tcacgtatgg caggcgttcg cggcaatgct tcccgaggUc 840 cagcaggcga tcgagcggat tggcgagttt ttacgccagc attggcagta a 891 <210> 172 <211> 296 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 172 Met Ala Ser Glu Ala Leu Thr Met Ile Val Asn Leu Leu Arg Ser Gin Arg Pro Leu Gln Glu Pro Thr Val Glu Gln Met Arg Ala Gly Leu Glu Sequence Listing (2) Ala Met Ala Gln Met Ser Pro Leu Pro Ala Asp Val Glu Leu Thr Thr Val Asp Ala Gly Gly Val Pro Gly Ala Trp Val Arg Val Pro Glu Ser Asp Pro Asp Arg Val Val Leu Tyr Leu His Gly Gly Gly Tyr Val Ile Gly Ser Ile Arg Thr His Arg Asp Leu Ala Gln Arg Ile Ala Arg Ala Ala Arg Cys Arg Val Leu Leu Ile Asp Tyr Arg Leu Ala Pro Glu His Pro His Pro Ala Ala Val Glu Asp Ser Thr Arg Ala Tyr Arg Trp Leu Leu Glu Thr Gly Ser Ala Pro Lys Arg Met Ala Ile Ala Gly Asp Ser Ala Gly Gly Gly Leu Thr Val Ala Thr Leu Val Ala Leu Arg Asp Ala Gly Val Pro Leu Pro Ala Ala Ala Val Cys Leu Ser Pro Trp Val Asp Leu Glu Gly Ile Gly Glu Ser Met Thr Thr Lys Ala Ala Val Asp Pro Met Val Gln Arg Glu Pro Leu Leu Arg Met Ala Ser Met Tyr Leu Ala Gly Gln Asp Pro Arg Thr Pro Leu Ala Ala Pro Leu Tyr Ala Asp Leu Arg Glu Leu Pro Pro Leu Leu Ile Gln Val Gly Thr Ala Glu Thr Leu Leu Asp Asp Ser Met Arg Leu Ala Glu Arg Ala Arg Ala Ala Gly Val Sequence Listing (2) Gln Val Thr Leu Glu Pro Trp Glu Asp Met Ile His Val Trp Gln Ala Phe Ala Ala Met Leu Pro Glu Gly Gln Gln Ala Ile Glu Arg Ile Gly Glu Phe Leu Arg Gln His Trp Gln <210> 173 <211> 924 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 173 gtggatccga aggcccagat cgtcggcgag ttcgtcaagt ccatccgcca gccgggttac 60 tttcccccgc tacccgagct gcgccagcag ctccggacga tggtggcgct gatggacgag 120 ccggcacccg cgctggcgcg catcgaggac cgccgcatcc cgggcccggc cggcgacat:a 180 ccggtgcgcg tgtacgcctc ggacgtcggc gcctcgcgtc ccgtcgtggc gtactttcac 240 ggcggcggct gggtgcaggg cgacctcgag actcaccacg gcctctgcgc gaggctggcg 300 aagcacgccg gggccctcgt cgtggccgtc gactaccggc tggcgcccga gcacaagttc 360 ccggccgccg tcgaggactg cctggcggcc tatcgctggc tccgagccca cggaggcgac 420 ctcggcgccg acccggggcg cgtggcggtg gccggggact cggcgggcgg caatctctcg 480 gccgtcgtct cgcaggtcgc agccgcggag ggcgctccgg tgcccacgtg ccaggtcct:g 540 atctatccgg cggtggactt ctcgctcgac agcgactccc accgagagct ggcggaggqc 600 cacatcatcc cgcgcgaccg tgtcctctgg tactcggagc agtacctgag gggcgaggcc 660 gacaagcggg atctgcgcgc ctcgcccctg cgggcgccga agctcgccgg ccagccgccg 720 acgctcgtcg tcaccgccgg gttcgatcct ctccgcgacg agggccgggc gtatgccgat 780 cggctccgcg aggccggggt ggacgtcgtc taccgcgagt acccgggcca gatccatgcc 840 ttcgtgtcgc tgacgaaggc gattccggag ggcatggcct gcacgctgga gattgccgag 900 Sequence Listing (2) tacctgcgcc agcgcctcgg ctga 924 <210> 174 <211> 307 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 174 Met Asp Pro Lys Ala Gln Ile Val Gly Glu Phe Val Lys Ser Ile Arg Gln Pro Gly Tyr Phe Pro Pro Leu Pro Glu Leu Arg Gln Gln Leu Arg Thr Met Val Ala Leu Met Asp Glu Pro Ala Pro Ala Leu Ala Arg Ile Glu Asp Arg Arg Ile Pro Gly Pro Ala Gly Asp Ile Pro Val Arg Val Tyr Ala Ser Asp Val Gly Ala Ser Arg Pro Val Val Ala Tyr Phe His Gly Gly Gly Trp Val Gln Gly Asp Leu Glu Thr His His Gly Leu Cys Ala Arg Leu Ala Lys His Ala Gly Ala Leu Val Val Ala Val Asp Tyr Arg Leu Ala Pro Glu His Lys Phe Pro Ala Ala Val Glu Asp Cys Leu Ala Ala Tyr Arg Trp Leu Arg Ala His Gly Gly Asp Leu Gly Ala Asp Pro Gly Arg Val Ala Val Ala Gly Asp Ser Ala Gly Gly Asn Leu Ser Ala Val Val Ser Gln Val Ala Ala Ala Glu Gly Ala Pro Val Pro Thr Sequence Listing (2) Cys Gln Val Leu Ile Tyr Pro Ala Val Asp Phe Ser Leu Asp Ser Asp Ser His Arg Glu Leu Ala Glu Gly His Ile Ile Pro Arg Asp Arg Val Leu Trp Tyr Ser Glu Gln Tyr Leu Arg Gly Glu Ala Asp Lys Arg Asp Leu Arg Ala Ser Pro Leu Arg Ala Pro Lys Leu Ala Gly Gln Pro Pro Thr Leu Val Val Thr Ala Gly Phe Asp Pro Leu Arg Asp Glu Gly Arg Ala Tyr Ala Asp Arg Leu Arg Glu Ala Gly Val Asp Val Val Tyr Arg Glu Tyr Pro Gly Gln Ile His Ala Phe Val Ser Leu Thr Lys Ala Ile Pro Glu Gly Met Ala Cys Thr Leu Glu Ile Ala Glu Tyr Leu Arg Gln Arg Leu Gly <210> 175 <211> 909 <212> DNA
<213> Pyrolobus fumarius <400> 175 atggctgttc agattaaaca aggacgcatt ggctttattg gggatgatcc aatcgaatca 60 cgccgacaat tcgatgcgca aatgttggcg attttaaata aatcaacatc gattgattc:a 120 gttgaagata ttcgcttacc actacaaagt ggaactgttt tcgcaagaca ctatcatcct 180 gcaccaaata aaaaacttcc aatgattgta ttttatcatg gaggaggttt tgttgtgggt 240 ggtttagata ctcatgatga agcctgtcga ctcattgcca aatatgcaaa agtacaagt.t 300 Sequence Listing (2) ttaagtattg actatccact tgcgccagaa gtgtcgccgc aactcctaat taaatcatgt 360 gaagatgcat tagcatgggt ttatcaaaac cgccgtcagt tgaaaattta taaaaaccgt 420 attgcggttg ctggcgatag cgcaggtgga aatattagta ctgttgttgc acagcatgcc 480 gcaggtaagt cttacgcacc gcaagcacaa ttacttattt atccagttgt cgatttcaaa 540 agtcgacatc cttcatttta tgcatatggc gaagggctgg tgttaacaag taaagatgta 600 gactacgtta cggattacta tgcaacgcag cacaatgttg ctttggatga cgcacttatt 660 tctccaactt atggcagttt taaaaagctt gcgccagcat acgtgattac tgctggacat 720 gacttactgc atgatgaagg tgagatatat agccataagc tcagacaaag cggtgttaaa 780 gtgcaataca tcgattaccc tgaacaaact cacggtttta ttaatctgac tccaatctcg 840 agcaaggcta aacggaatac gatcgaaatt gccaaaagct tcagaaagtt ttgggataag 900 catcactga 909 <210> 176 <211> 302 <212> PRT
<213> Pyrolobus fumarius <400> 176 Met Ala Val Gln Ile Lys Gln Gly Arg Ile Gly Phe Ile Gly Asp Asp Pro Ile Glu Ser Arg Arg Gln Phe Asp Ala Gln Met Leu Ala Ile Leu Asn Lys Ser Thr Ser Ile Asp Ser Val Glu Asp Ile Arg Leu Pro Leu Gln Ser Gly Thr Val Phe Ala Arg His Tyr His Pro Ala Pro Asn Lys Lys Leu Pro Met Ile Val Phe Tyr His Gly Gly Gly Phe Val Val Gly Gly Leu Asp Thr His Asp Glu Ala Cys Arg Leu Ile Ala Lys Tyr Ala Sequence Listing (2) Lys Val Gln Val Leu Ser Ile Asp Tyr Pro Leu Ala Pro Glu Val Ser Pro Gln Leu Leu Ile Lys Ser Cys Glu Asp Ala Leu Ala Trp Val Tyr Gln Asn Arg Arg Gln Leu Lys Ile Tyr Lys Asn Arg Ile Ala Val Ala Gly Asp Ser Ala Gly Gly Asn Ile Ser Thr Val Val Ala Gln His Ala Ala Gly Lys Ser Tyr Ala Pro Gln Ala Gln Leu Leu Ile Tyr Pro Val Val Asp Phe Lys Ser Arg His Pro Ser Phe Tyr Ala Tyr Gly Glu Gly Leu Val Leu Thr Ser Lys Asp Val Asp Tyr Val Thr Asp Tyr Tyr Ala Thr Gln His Asn Val Ala Leu Asp Asp Ala Leu Ile Ser Pro Thr Tyr Gly Ser Phe Lys Lys Leu Ala Pro Ala Tyr Val Ile Thr Ala Gly His Asp Leu Leu His Asp Glu Gly Glu Ile Tyr Ser His Lys Leu Arg Gln Ser Gly Val Lys Val Gln Tyr Ile Asp Tyr Pro Glu Gln Thr His Gly Phe Ile Asn Leu Thr Pro Ile Ser Ser Lys Ala Lys Arg Asn Thr Ile Glu Ile Ala Lys Ser Phe Arg Lys Phe Trp Asp Lys His His <210> 177 Sequence Listing (2) <211> 780 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 177 atgataaaaa acttcgacag agaaaattct agcttagtac tgtccggtgg tggtgctctg 60 ggtattgctc acttgggtgt actgcatgac cttgaaaaac aaaatattgt accaaatgaa 120 attgttggta caagtatggg tggtatcatt ggtgcatcta tggctatcgg gatgaaagag 180 aaagaaatac tcgaagaaat caaaaacttt tccaatgtct tcaactggat aaaattctct 240 ttttccggta attctgttgt cgataacgag aagatcgcta agatatttga tactcttttt 300 aaagacagaa agatgacaga tacggtgatc cctcttaaac tcatcgctac aaacttacat 360 aatggacata aaaaagtatt tactgcttcg gatgatgtac tgatcaaaga tgcaatactc 420 tcaacaatgg caatacccgg tgtatttgaa gaacatatta ttgatggtga aacctatggc 480 gacggttttc tttgtgaaaa ccttggtgtg aatgaggcaa cattcaatga tgttttagct 540 gtagatgtca tgggtgagaa ctcttttgaa aaagcaatgc cggacaactt ctttaaaaca 600 tcaaatgttt tagaaatgtt tgaaaaatca atgcgacttt ttatttacaa ccagacacag 660 acacatatta aaaatgcaaa taaaaatatt tatcttattg aacccgttac caaagagtat 720 aaaacatttc aatttcataa acataaagag atacgtgctt taggcttggg tttactgtaa 780 <210> 178 <211> 259 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 178 Met Ile Lys Asn Phe Asp Arg Glu Asn Ser Ser Leu Val Leu Ser Gly Gly Gly Ala Leu Gly Ile Ala His Leu Gly Val Leu His Asp Leu Glu Lys Gln Asn Ile Val Pro Asn Glu Ile Val Gly Thr Ser Met Gly Gly Sequence Listing (2) Ile Ile Gly Ala Ser Met Ala Ile Gly Met Lys Glu Lys Glu Ile Leu Glu Glu Ile Lys Asn Phe Ser Asn Val Phe Asn Trp Ile Lys Phe Ser Phe Ser Gly Asn Ser Val Val Asp Asn Glu Lys Ile Ala Lys Ile Phe Asp Thr Leu Phe Lys Asp Arg Lys Met Thr Asp Thr Val Ile Pro Leu Lys Leu Ile Ala Thr Asn Leu His Asn Gly His Lys Lys Val Phe Thr Ala Ser Asp Asp Val Leu Ile Lys Asp Ala Ile Leu Ser Thr Met Ala Ile Pro Gly Val Phe Glu Glu His Ile Ile Asp Gly Glu Thr Tyr Gly Asp Gly Phe Leu Cys Glu Asn Leu Gly Val Asn Glu Ala Thr Phe Asn Asp Val Leu Ala Val Asp Val Met Gly Glu Asn Ser Phe Glu Lys Ala Met Pro Asp Asn Phe Phe Lys Thr Ser Asn Val Leu Glu Met Phe Glu Lys Ser Met Arg Leu Phe Ile Tyr Asn Gln Thr Gln Thr His Ile Lys Asn Ala Asn Lys Asn Ile Tyr Leu Ile Glu Pro Val Thr Lys Glu Tyr Lys Thr Phe Gin Phe His Lys His Lys Glu Ile Arg Ala Leu Gly Leu Sequence Listing (2) Gly Leu Leu <210> 179 <211> 1413 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 179 atgggtatct ttgattataa aaacctcggg accgagggct caaaagcatt gttcgccgat 60 gccatggcga tcacgttgta ttcctatcac aacctggata acggctttgc cgtggggtac 120 cagcacaacg gcctggggct gggcttgcca gctacgttgg tcggggcgct gttgggcagc 180 acggactccc agggcgtaat ccccggcatc ccctggaacc cggactccga aaaagccgcg 240 ctggacgcgg tacacaaggc cggttggacg cccatcagcg ccagcaccct gggttacagt 300 ggcaacgtcg atgcgcgagg caccttcttt ggcgagaaga cgggctacac cacggcccag 360 gtcgaagtgc tgggcaagta cgatgacgcc ggaaaactgc tggaaatcgg cattggtttt 420 cgcggcactt cggggcctcg ggaaaccttg atcagcgatt ccatcggtga cctggtcagc 480 gacctgctgg ccgccctggg ccccaaggat tacgcgaaga attacgccgg ggaagccttc 540 ggcgggttgc tcaagaatgt ggccgactac gccagcgccc atggcttgag cggtcacga.t 600 gtggtggtca gcggccacag cctgggcggg ctggcagtca acagcatggc cgacctgagc 660 aacagcaagt ggtcggggtt ctacaaggac gcgaactacg tggcctacgc ctcgccgac:c 720 cagagtgcca gcgacaaggt gctcaacatc ggctacgaga atgacccggt gttccgtgc:g 780 ctggatggct catccttcac cctgt.cttcc ctgggtgtgc acgacacccc ccatgagtc:c 840 accaccgaca atattgtcag tttcaacgac cactacgcct cgacgctgtg gaatgtgctg 900 ccgttttcca ttgtcaatct gccgacttgg gtttcgcacc tgcctaccgg gtacggcgat 960 ggcctgagtc gcgtgctcga ctccgggttc tatgagcaaa tgacccgcga ctccgccgtg 1020 atcgtcgcca acctgtccga cccggcacgg gccaccacct gggtgcagga cctcaaccgc 1080 aatgccgagc cgcataaggg caataccttc atcatcggca gcgacggcca cgacctgatc 1140 cagggcggca agggtgcgga ctttatcgaa ggcggcaaag gcaatgacac gattcgtgac 1200 Sequence Listing (2) aacagcgggc acaacacctt tttgttcagc ggtcagtttg gccaggaccg gatcatcggc 1260 tatcagccaa gcgatcaact ggtattcaag gaggtagagg ggagtgctca ataccgtgag 1320 cacggagggg acacagtgat cagcttcggc gcagattcgg tgacattggt gggggtcaac 1380 gggtggtcgg gggagggcgt tgccatcggt tga 1413 <210> 180 <211> 470 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 180 Met Gly Ile Phe Asp Tyr Lys Asn Leu Gly Thr Glu Gly Ser Lys Ala Leu Phe Ala Asp Ala Met Ala Ile Thr Leu Tyr Ser Tyr His Asn Leu Asp Asn Gly Phe Ala Val Gly Tyr Gln His Asn Gly Leu Gly Leu Gly Leu Pro Ala Thr Leu Val Gly Ala Leu Leu Gly Ser Thr Asp Ser Gin Gly Val Ile Pro Gly Ile Pro Trp Asn Pro Asp Ser Glu Lys Ala Ala Leu Asp Ala Val His Lys Ala Gly Trp Thr Pro Ile Ser Ala Ser Thr Leu Gly Tyr Ser Gly Asn Val Asp Ala Arg Gly Thr Phe Phe Gly Glu Lys Thr Gly Tyr Thr Thr Ala Gln Val Glu Val Leu Gly Lys Tyr Asp Asp Ala Gly Lys Leu Leu Glu Ile Gly Ile Gly Phe Arg Gly Thr Ser Sequence Listing (2) Gly Pro Arg Glu Thr Leu Ile Ser Asp Ser Ile Gly Asp Leu Val Ser Asp Leu Leu Ala Ala Leu Gly Pro Lys Asp Tyr Ala Lys Asn Tyr Ala Gly Glu Ala Phe Gly Gly Leu Leu Lys Asn Val Ala Asp Tyr Ala Ser Ala His Gly Leu Ser Gly His Asp Val Val Val Ser Gly His Ser Leu Gly Gly Leu Ala Val Asn Ser Met Ala Asp Leu Ser Asn Ser Lys Trp Ser Gly Phe Tyr Lys Asp Ala Asn Tyr Val Ala Tyr Ala Ser Pro Thr Gln Ser Ala Ser Asp Lys Val Leu Asn Ile Gly Tyr Glu Asn Asp Pro Val Phe Arg Ala Leu Asp Gly Ser Ser Phe Thr Leu Ser Ser Leu Gly Val His Asp Thr Pro His Glu Ser Thr Thr Asp Asn Ile Val Ser Phe Asn Asp His Tyr Ala Ser Thr Leu Trp Asn Val Leu Pro Phe Ser Ile Val Asn Leu Pro Thr Trp Val. Ser His Leu Pro Thr Gly Tyr Gly Asp Gly Leu Ser Arg Val Leu Asp Ser Gly Phe Tyr Glu Gln Met Thr Arg Asp Ser Ala Val Ile Val Ala Asn Leu Ser Asp Pro Ala Arg Ala Thr Thr Trp Val Gln Asp Leu Asri Arg Asn Ala Glu Pro His Lys Gly Asn Sequence Listing (2) Thr Phe Ile Ile Gly Ser Asp Gly His Asp Leu Ile Gln Gly Gly Lys Gly Ala Asp Phe Ile Glu Gly Gly Lys Gly Asn Asp Thr Ile Arg Asp Asn Ser Gly His Asn Thr Phe Leu Phe Ser Gly Gln Phe Gly Gln Asp Arg Ile Ile Gly Tyr Gln Pro Ser Asp Gln Leu Val Phe Lys Glu Val Glu Gly Ser Ala Gln Tyr Arg Glu His Gly Gly Asp Thr Val Ile Ser Phe Gly Ala Asp Ser Val Thr Leu Val Gly Val Asn Gly Trp Ser Gly Glu Gly Val Ala Ile Gly <210> 181 <211> 939 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 181 atgccactcg acccaaaagc caaagcgttt ttagaccagg ccgctgcggc tggcgcgcca 60 gcactggacg cgcttcccgt tcgagaagct cgcgaggcga ttcgcggcct gtttgcggcc 120 ataggagaaa aagaacccgt caaacaaata gaagatcgca tgatccctgg cccgggcgga 180 cagatgcctg tgcgtatgta tacgccggca ggtaccacgc cttttcccgt cctcgtttat 240 ttccatggcg gaggatgggt cctgggtgat ctagaaactc acgatgcgac ctgccgggcg 300 ctggcaaatg ctgctggctg catggtgatc tcggttgact atcgtttggc gccggagcac 360 aaattcccta ccgctcccga ggattgctat gcggccacca aatgggcagc gctgaatgcg 420 Sequence Listing (2) gcggctcttg gcggagatcc aaccaagatt gccgttggtg gcgatagtgc cggagggaat 480 cttgctgccg cagtggcaca aatggccagt gaccgtgggg ctccttcgct cgggtttcag 540 ttgctcatct atccagtgac caattacgcg ttcgataccg cctcataccg ggcaaatgcg 600 gagggctacc tgctcacaaa aagcgcgatg gggtggttct ggaaccatta tctgcagaac 660 gaaaccgatg gccagaatcc ctatgcttcg cctttacgtg gacaatatct gcgcaatcta 720 cccccagcac ttgtcatcac cgcggagtat gacccgctcc gcgacgaggg agaagcgtat 780 gcagcgcggc tgcgcgaagc aggtgttgct gtcactcaca ctgactacaa aggcatgatt 840 catggcctct tcagcatgac caacgtagtc gatcaggcga agcaagccat caatgaagct 900 ggtgccgcac tgaaggcagc gtttgcggcg aaacgataa 939 <210> 182 <211> 312 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 182 Met Pro Leu Asp Pro Lys Ala Lys Ala Phe Leu Asp Gln Ala Ala Ala Ala Gly Ala Pro Ala Leu Asp Ala Leu Pro Val Arg Glu Ala Arg Glu Ala Ile Arg Gly Leu Phe Ala Ala Ile Gly Glu Lys Glu Pro Val Lys Gln Ile Glu Asp Arg Met Ile Pro Gly Pro Gly Gly Gln Met Pro Val Arg Met Tyr Thr Pro Ala Gly Thr Thr Pro Phe Pro Val Leu Val Tyr Phe His Gly Gly Gly Trp Val Leu Gly Asp Leu Glu Thr His Asp Ala Thr Cys Arg Ala Leu Ala Asn Ala Ala Gly Cys Met Val Ile Ser Val Sequence Listing (2) Asp Tyr Arg Leu Ala Pro Glu His Lys Phe Pro Thr Ala Pro Glu Asp Cys Tyr Ala Ala Thr Lys Trp Ala Ala Leu Asn Ala Ala Ala Leu Gly Gly Asp Pro Thr Lys Ile Ala Val Gly Gly Asp Ser Ala Gly Gly Asn Leu Ala Ala Ala Val Ala Gln. Met Ala Ser Asp Arg Gly Ala Pro Ser Leu Gly Phe Gln Leu Leu Ile Tyr Pro Val Thr Asn Tyr Ala Phe Asp Thr Ala Ser Tyr Arg Ala Asn Ala Glu Gly Tyr Leu Leu Thr Lys Ser Ala Met Gly Trp Phe Trp Asn His Tyr Leu Gln Asn Glu Thr Asp Gly Gln Asn Pro Tyr Ala Ser Pro Leu Arg Gly Gln Tyr Leu Arg Asn Leu Pro Pro Ala Leu Val Ile Thr Ala Glu Tyr Asp Pro Leu Arg Asp Glu Gly Glu Ala Tyr Ala Ala Arg Leu Arg Glu Ala Gly Val Ala Val Thr His Thr Asp Tyr Lys Gly Met Ile His Gly Leu Phe Ser Met Thr Asn Val Val Asp Gln Ala Lys Gln Ala Ile Asn Glu Ala Gly Ala Ala Leu Lys Ala Ala Phe Ala Ala Lys Arg Sequence Listing (2) <210> 183 <211> 1431 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 183 atgggtatct ttgactataa aaacctcggc accgagggtt ccaaagcgct gtttgccgac 60 gccatggcga tcacgttgta ctcctaccac aacctggata acggctttgc cgtgggttac 120 cagcacaatg gcttggggct tggtttgccg gccacgctgg tgggtgcact gctcggcagc 180 acgaattcac agggtgtcat tccgggcatt ccctggaacc cggattcgga aaaagccgcg 240 ctggaggcgg tgcaaaaagc cggttggacg ccgatcagtg caagtaccct cggctacggc 300 ggcaaagtcg atgcgcgcgg cacgtttttt ggcgaaaaag ccggctacac cacggcgcag 360 gtcgaggtgc tgggcaagta cgatgacgcc ggcaaactgc tggaaatcgg catcggcttt 420 cgcggcacgt cggggccgcg ggaaaccttg atcagcgact ccatcggcga cctggtcagc 480 gatctgcttg cggcgctggg gcccaaggat tacgcaaaga actacgccgg cgaagcgttc 540 ggcgggctgc tcaaaaacgt cgccgattac gccgctgccc atggccttac cggcaaggac 600 gtggtggtca gcggccacag cctgggcggc ctggcggtca acagcatggc ggatttaagc 660 accaacaaat ggtcgggctt ctacacggac gccaactacg tggcctatgc atcgccgacc 720 caaagcgccg gcgacaaggt gctcaacatc ggctatgaaa acgacccggt gtttcgcgcg 780 ctggacggct catccttcaa tctgtcgtcc ctgggcgtgc acgacaagcc ccacgagtcg 840 accaccgata acatcgtcag cttcaacgac cactacgcct cgacgctgtg gaatgtgctg 900 ccgttctcca tcgtcaacct gccgacctgg gtctcgcacc tgccgaccgc atacggcgac 960 ggcatgacgc gcatcctcga ctccgggttc tatgaccaga tgacccgtga ctccacggta 1020 atcgtcgcca acctgtccga cccggcgcgg gcgactacgt gggtgcagga cctcaaccgc 1080 aatgccgaac cgcacaaggg caataccttc attattggca gcgacggcaa cgacctgatc 1140 cagggtggca agggcgcgga ctttatcgaa ggcggcaaag gcaatgacac gatccgcgac 1200 aacagtgggc ataacacctt cctgttcagt ggccagttcg gcaacgatcg cgtgattggc 1260 taccagacca ccgacaagct ggtgttccag gacgtgcagg ggagcaccga cctgcgtgac 1320 Sequence Listing (2) catgccaaag tggtcggcgc ggacacggtg ctcacgtttg gcgccgactc ggtgaccctg 1380 gttggcgtcg gccatggcgg cctgtgggcg gacggcgtca gcatcggctg a 1431 <210> 184 <211> 476 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 184 Met Gly Ile Phe Asp Tyr Lys Asn Leu Gly Thr Glu Gly Ser Lys Ala Leu Phe Ala Asp Ala Met Ala Ile Thr Leu Tyr Ser Tyr His Asn Leu Asp Asn Gly Phe Ala Val Gly Tyr Gln His Asn Gly Leu Gly Leu Gly Leu Pro Ala Thr Leu Val Gly Ala Leu Leu Gly Ser Thr Asn Ser Gln Gly Val Ile Pro Gly Ile Pro Trp Asn Pro Asp Ser Glu Lys Ala Ala Leu Glu Ala Val Gln Lys Ala Gly Trp Thr Pro Ile Ser Ala Ser Thr Leu Gly Tyr Gly Gly Lys Val Asp Ala Arg Gly Thr Phe Phe Gly Glu Lys Ala Gly Tyr Thr Thr Ala Gln Val Glu Val Leu Gly Lys Tyr Asp Asp Ala Gly Lys Leu Leu Glu Ile Gly Ile Gly Phe Arg Gly Thr Ser Gly Pro Arg Glu Thr Leu Ile Ser Asp Ser Ile Gly Asp Leu Val Ser Sequence Listing (2) Asp Leu Leu Ala Ala Leu Gly Pro Lys Asp Tyr Ala Lys Asn Tyr Ala Gly Glu Ala Phe Gly Gly Leu Leu Lys Asn Val Ala Asp Tyr Ala Ala Ala His Gly Leu Thr Gly Lys Asp Val Val Val Ser Gly His Ser Leu Gly Gly Leu Ala Val Asn Ser Met Ala Asp Leu Ser Thr Asn Lys Trp Ser Gly Phe Tyr Thr Asp Ala Asn Tyr Val Ala Tyr Ala Ser Pro Thr Gln Ser Ala Gly Asp Lys Val Leu Asn Ile Gly Tyr Glu Asn Asp Pro Val Phe Arg Ala Leu Asp Gly Ser Ser Phe Asn Leu Ser Ser Leu Gly Val His Asp Lys Pro His Glu Ser Thr Thr Asp Asn Ile Val Ser Phe Asn Asp His Tyr Ala Ser Thr Leu Trp Asn Val Leu Pro Phe Ser Ile Val Asn Leu Pro Thr Trp Val. Ser His Leu Pro Thr Ala Tyr Gly Asp Gly Met Thr Arg Ile Leu Asp Ser Gly Phe Tyr Asp Gln Met Thr Arg Asp Ser Thr Val Ile Val Ala Asn Leu Ser Asp Pro Ala Arg Ala Thr Thr Trp Val Gln Asp Leu Asn Arg Asn Ala Glu Pro His Lys Gly Asn Thr Phe Ile Ile Gly Ser Asp Gly Asn Asp Leu Ile Gln Gly Gly Lys Sequence Listing (2) Gly Ala Asp Phe Ile Glu Gly Gly Lys Gly Asn Asp Thr Ile Arg Asp Asn Ser Gly His Asn Thr Phe Leu Phe Ser Gly Gln Phe Gly Asn Asp Arg Val Ile Gly Tyr Gln Thr Thr Asp Lys Leu Val Phe Gln Asp Val Gln Gly Ser Thr Asp Leu Arg Asp His Ala Lys Val Val Gly Ala Asp Thr Val Leu Thr Phe Gly Ala Asp Ser Val Thr Leu Val Gly Val Gly His Gly Gly Leu Trp Ala Asp Gly Val Ser Ile Gly <210> 185 <211> 1038 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 185 atgaaacgaa tcgcaaccgc cgtgtttttg ctgcatgcca tgacgtcggt ggccgtgtgc 60 gccgacgtcg cgggcgatac ccagaaacag atatggggac gctacctgga aaggagcgaa 120 gaagccgcgc tcacggcaat gataccgcaa caaaccccgc cgccggaaac ccccgttgac 180 aaatcggccc gggaacgcat cggcgaaacc aggaaagcgt tcctgaatat gacggcgga.g 240 ttcgcgactt cgctggcgga cattccgacg acggagtatg accaggaggg tatccgggga 300 agctggatca tgcccaatga tgcggattcc gaacgcgtgc ttttgtatct tcacggcggc 360 ggctacatag tcggaagcga tgaaacgccc cgcgcgataa ccgcatttct ggcgcgcgag 420 gcaaaagtgc ggtgtttttc cctggattat ccgcttgccc ccgagcatcc atttccggc.t 480 gcgctcgata gcgcggtgcg atcgtatgaa atgctgttgg aaaaagggtt cagccctgga 540 Sequence Listing (2) aacatcgtgc tgggcggcga ttcggccggc ggcggcctgg cgcttgccct gttgctcgcc 600 atccgggaac acgggctgcc gatgccggcg ggagcgtatc tcctttcccc gtggactgat 660 ttgacgcaga gttttcccac gcattcacgc aaggccgatg tcgaaatcag cattaccccc 720 gagctgcttg aggaagcggc agccagctat gcgggtgatt ccgacagaac caatccgctt 780 atctcccccg cattcggcga gttccgggga ttcccgccgc tcctgataca agttggttcc 840 catgaacgtc tgttggacga ctccctgacg gtggcgcgca atgccgccct cgccgatgtg 900 ccgacgacat tgaaggtatg gcccggctat ccccatgtgt ttcaagtcta tcaccagaat 960 ctcgacggcg ccaaaaaggc gttgagggaa gccgcggaat tcatcaccgg agcgatggat 1020 aaagagttgc ttcaataa 1038 <210> 186 <211> 345 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 186 Met Lys Arg Ile Ala Thr Ala Val Phe Leu Leu His Ala Met Thr Ser Val Ala Val Cys Ala Asp Val. Ala Gly Asp Thr Gln Lys Gln Ile Trp Gly Arg Tyr Leu Glu Arg Ser Glu Glu Ala Ala Leu Thr Ala Met Ile Pro Gln Gln Thr Pro Pro Pro Glu Thr Pro Val Asp Lys Ser Ala Arg Glu Arg Ile Gly Glu Thr Arg Lys Ala Phe Leu Asn Met Thr Ala Glu Phe Ala Thr Ser Leu Ala Asp Ile Pro Thr Thr Glu Tyr Asp Gln Glu Gly Ile Arg Gly Ser Trp Ile Met Pro Asn Asp Ala Asp Ser Glu Arg Sequence Listing (2) Val Leu Leu Tyr Leu His Gly Gly Gly Tyr Ile Val Gly Ser Asp Glu Thr Pro Arg Ala Ile Thr Ala Phe Leu Ala Arg Glu Ala Lys Val Arg Cys Phe Ser Leu Asp Tyr Pro Leu Ala Pro Glu His Pro Phe Pro Ala Ala Leu Asp Ser Ala Val Arg Ser Tyr Glu Met Leu Leu Glu Lys Gly Phe Ser Pro Gly Asn Ile Val. Leu Gly Gly Asp Ser Ala Gly Gly Gly Leu Ala Leu Ala Leu Leu Leu Ala Ile Arg Glu His Gly Leu Pro Met Pro Ala Gly Ala Tyr Leu Leu Ser Pro Trp Thr Asp Leu Thr Gln Ser Phe Pro Thr His Ser Arg Lys Ala Asp Val Glu Ile Ser Ile Thr Pro Glu Leu Leu Glu Glu Ala Ala Ala Ser Tyr Ala Gly Asp Ser Asp Arg Thr Asn Pro Leu Ile Ser Pro Ala Phe Gly Glu Phe Arg Gly Phe Pro Pro Leu Leu Ile Gln Val Gly Ser His Glu Arg Leu Leu Asp Asp Ser Leu Thr Val Ala Arg Asn Ala Ala Leu Ala Asp Val Pro Thr Thr Leu Lys Val Trp Pro Gly Tyr Pro His Val Phe Gln Val Tyr His Gln Asn Sequence Listing (2) Leu Asp Gly Ala Lys Lys Ala Leu Arg Glu Ala Ala Glu Phe Ile Thr Gly Ala Met Asp Lys Glu Leu Leu Gln <210> 187 <211> 1026 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 187 gtgaaacatt atgtaattgc actgacaacc gccttcctgc tctatacagc cttgccggct 60 acggctgcgg atacgagccg gagctatccc gttccgaaca ccgtcagccc ggaactgcaa 120 acgctcatcg gttccgctcc gtttccatac tggaacaggc acccggccaa tgcggaagaa 180 tggaaggcct ttgtcaagga actctcggat gccgccgcgc cgggaatacc cgtgttgcgg 240 gaaaaaatgg gcgtcacctc cacacccggc gttatggccg gggtaccggt cttcaccata 300 accccgcgga atctgcccgc cgccaatgcg gatcgcgtgc tgctccattt tcatggcggg 360 ggatatgtgc tggggcctgg agaaaccgga accgacgaag ccattctgat ggcgggagtc 420 tccgggtacc gggtcatttc cgtggattac agaatggcgc cggatcatcc ttacccggcg 480 gccatagacg atgccgtcgc ggtctacaag gaacttctca aaaccgtgcc tgccggaaag 540 atcggcgtct tcggcacctc cacaggcgga ggcatgacgc tcatcctggc cctgcgcgcg 600 aaagccgaag ggttacctat gcccggcgct ctcggcgcgg gaacgccgtg gactgatat.g 660 accaaaaccg gtgataccta cttcaccaac gagtatctcg ataacgtttt ggtcagttac 720 gacggatggg tcggtgatgc ggccaaaata tacgccgccg ggcatgacct gaaagacccc 780 atgctttctc cggtgtatgg cgatgtttcc ggctttccac ccacactgct gacatcgggc 840 acccgcgacc tttttctgag caacacagtg cgcatgcacc agaagctacg gcaagcggga 900 gctacggcgg acttgatcgt ttttgaaggc atgtcccact gccattacta ctttcttcc:t 960 gatgcgcctg aaacccgctt ccacttcacg gagctcggta aattcttcgg acgtcattta 1020 cagtaa 1026 Sequence Listing (2) <210> 188 <211> 341 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 188 Met Lys His Tyr Val Ile Ala Leu Thr Thr Ala Phe Leu Leu Tyr Thr Ala Leu Pro Ala Thr Ala Ala Asp Thr Ser Arg Ser Tyr Pro Val Pro Asn Thr Val Ser Pro Glu Leu Gln Thr Leu Ile Gly Ser Ala Pro Phe Pro Tyr Trp Asn Arg His Pro Ala Asn Ala Glu Glu Trp Lys Ala Phe Val Lys Glu Leu Ser Asp Ala Ala Ala Pro Gly Ile Pro Val Leu Arg Glu Lys Met Gly Val Thr Ser Thr Pro Gly Val Met Ala Gly Val Pro Val Phe Thr Ile Thr Pro Arg Asn Leu Pro Ala Ala Asn Ala Asp Arg Val Leu Leu His Phe His Gly Gly Gly Tyr Val Leu Gly Pro Gly Glu Thr Gly Thr Asp Glu Ala Ile Leu Met Ala Gly Val Ser Gly Tyr Arg Val Ile Ser Val Asp Tyr Arg Met Ala Pro Asp His Pro Tyr Pro Ala Ala Ile Asp Asp Ala Val Ala. Val Tyr Lys Glu Leu Leu Lys Thr Val Sequence Listing (2) Pro Ala Gly Lys Ile Gly Val Phe Gly Thr Ser Thr Gly Gly Gly Met Thr Leu Ile Leu Ala Leu Arg Ala Lys Ala Glu Gly Leu Pro Met Pro Gly Ala Leu Gly Ala Gly Thr Pro Trp Thr Asp Met Thr Lys Thr Gly Asp Thr Tyr Phe Thr Asn Glu Tyr Leu Asp Asn Val Leu Val Ser Tyr Asp Gly Trp Val Gly Asp Ala Ala Lys Ile Tyr Ala Ala Gly His Asp Leu Lys Asp Pro Met Leu Ser Pro Val Tyr Gly Asp Val Ser Gly Phe Pro Pro Thr Leu Leu Thr Ser Gly Thr Arg Asp Leu Phe Leu Ser Asn Thr Val Arg Met His Gln Lys Leu Arg Gln Ala Gly Ala Thr Ala Asp Leu Ile Val Phe Glu Gly Met Ser His Cys His Tyr Tyr Phe Leu Pro Asp Ala Pro Glu Thr Arg Phe His Phe Thr Glu Leu Gly Lys Phe Phe Gly Arg His Leu Gln <210> 189 <211> 1347 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 189 Sequence Listing (2) atggaaaaat tagccgtttt atacgccgaa catattgaga cactgcaaca acgtactcgt 60 acaatttgtg aacgtgaagg gttagaggga ttagttattc actcgggcca agctaagcgt 120 cagtttttag atgatatgta ttatccattt aaagttaatc cacactttaa agcttggtta 180 ccagtactag acaatccaca ttgctggatt gtggtaaatg gtagcgacaa gcctaaactt 240 attttttatc gtccggttga tttttggcat aaagtgccag atgagccgcg cgatttttgg 300 gctgagtatt ttgacattga attgttatta cagcccgatc aggttgaaaa gctattgccc 360 tacgacaaag ctaactacgc ttatgtgggc gaatacttag aagtagcaca agcactaggc 420 tttagtgtta tgaatcctga gccggtacta aattactttc attaccaccg cgcttataaa 480 acccaatatg agcttgaatg tttacgcaac gccaaccgta ttgcagtaga tggccacaaa 540 gctgcccgcg ataccttttt taatggtggc agtgagtttg atattcagca agcttactta 600 atggcaactc gccaaagtga aaacgaaatg ccttatggca acattgtggc gctaaacgaa 660 aactgcgcca ttttgcacta cacgcacttt gaggcaaaag caccgcagca acacaactca 720 tttttaatag atgcaggcgc taattttaat ggctatgcgg cagatattac ccgtacgtat 780 gactttaaaa aacaaggcga atttgcagag ctagtgcaag caatgactgc agcgcaaata 840 gagttaggta cggggttaaa acccggcatg ctttatggtg atttacatgt tgagtgtcac 900 aatagagttg cacaaatact tagcgacttt gatattgtta agctaccggc tgaggaaata 960 gttgaacgta aaattacctc taccttcttc ccacatggtt tagggcacca cctaggttta 1020 caagtgcacg acatgggtgg ttttatggct gatgaaatgg gcgcacaaca agcagctcca 1080 gagggacatc catttttgcg ttgtactcgc ataattgaaa aaaaccaagt gtttactatt 1140 gagcctgggc tatactttat cgactctttg ctaggcgatt tagcacaaac cgataataaa 1200 cagtttatta attggcaaaa ggtagagtcg tttaagcctt atggtggtat tcgtattgaa 1260 gataacatca tagtacacga agatagcctt gagaatatga cacgaaattt agcgctagat 1320 agatctcatc accatcacca tcactaa 1347 <210> 190 <211> 448 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample Sequence Listing (2) <400> 190 Met Glu Lys Leu Ala Val Leu Tyr Ala Glu His Ile Glu Thr Leu Gln Gln Arg Thr Arg Thr Ile Cys Glu Arg Glu Gly Leu Glu Gly Leu Val Ile His Ser Gly Gln Ala Lys Arg Gln Phe Leu Asp Asp Met Tyr Tyr Pro Phe Lys Val Asn Pro His Phe Lys Ala Trp Leu Pro Val Leu Asp Asn Pro His Cys Trp Ile Val Val Asn Gly Ser Asp Lys Pro Lys Leu Ile Phe Tyr Arg Pro Val Asp Phe Trp His Lys Val Pro Asp Glu Pro Arg Asp Phe Trp Ala Glu Tyr Phe Asp Ile Glu Leu Leu Leu Gln Pro Asp Gln Val Glu Lys Leu Leu Pro Tyr Asp Lys Ala Asn Tyr Ala Tyr Val Gly Glu Tyr Leu Glu Val Ala Gln Ala Leu Gly Phe Ser Val Met Asn Pro Glu Pro Val Leu Asn Tyr Phe His Tyr His Arg Ala Tyr Lys Thr Gln Tyr Glu Leu Glu Cys Leu Arg Asn Ala Asn Arg Ile Ala Val Asp Gly His Lys Ala Ala Arg Asp Thr Phe Phe Asn Gly Gly Ser Glu Phe Asp Ile Gln Gln Ala Tyr Leu Met Ala Thr Arg Gln Ser Glu Asn Sequence Listing (2) Glu Met Pro Tyr Gly Asn Ile Val Ala Leu Asn Glu Asn Cys Ala Ile Leu His Tyr Thr His Phe Glu Ala Lys Ala Pro Gin Gln His Asn Ser Phe Leu Ile Asp Ala Gly Ala Asn Phe Asn Gly Tyr Ala Ala Asp Ile Thr Arg Thr Tyr Asp Phe Lys Lys Gln Gly Glu Phe Ala Glu Leu Val Gln Ala Met Thr Ala Ala Gln Ile Glu Leu Gly Thr Gly Leu Lys Pro Gly Met Leu Tyr Gly Asp Leu His Val Glu Cys His Asn Arg Val Ala Gln Ile Leu Ser Asp Phe Asp Ile Val Lys Leu Pro Ala Glu Glu Ile Val Glu Arg Lys Ile Thr Ser Thr Phe Phe Pro His Gly Leu Gly His His Leu Gly Leu Gln Val His Asp Met Gly Gly Phe Met Ala Asp Glu Met Gly Ala Gln Gln Ala Ala Pro Glu Gly His Pro Phe Leu Arg Cys Thr Arg Ile Ile Glu Lys Asri Gln Val Phe Thr Ile Glu Pro Gly Leu Tyr Phe Ile Asp Ser Leu Leu Gly Asp Leu Ala Gln Thr Asp Asn Lys Gln Phe Ile Asn Trp Gln Lys Val Glu Ser Phe Lys Pro Tyr Gly Gly Ile Arg Ile Glu Asp Asn Ile Ile Val His Glu Asp Ser Leu Glu Asn Sequence Listing (2) Met Thr Arg Asn Leu Ala Leu Asp Arg Ser His His His His His His <210> 191 <211> 1134 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 191 atgaccttga ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc 60 tcgcgcgcct gcaggtcgac actagtggat ccgaggatcg acccgcgcat caaggcggtg 120 atgggcgcgt tcgaggccac cagcgcgcag ggtgacgtcg ccagccgcga cgaactgctg 180 gccgaggcgg ccagcgacga agcggtggcc cagcgggaga tgctcaccgc cttcctcggc 240 atgtgcgaca ccgaggagat cgcgccgtcg gcggggctca cggtctccga gcaccgcgtc 300 gcctctcagc ccgacggcaa caagatcaac atccggttca tccggcccga gggcgacgac 360 gtgctgccgt gcgtgtacta catccacggc ggtggcatgg cgtcgatgtc gtgctatgac 420 ggcaactacc gggcgtgggg ccggatcatc gccgcccagg gcgtggcggt tgccatggtc 480 gacttccgca acgcgctcac accgtcgtcg gccgaggagg tggccccgtt ccccgctggg 540 ctcaacgact gcgtgtcggg gctcaagtgg gtcgcggcca acgccgccga tctgcgcatc 600 gacccggccc gcatcgtcgt ggccggcgag agcggcggcg ggaacctcac cctcgccacc 660 ggcctgaagc tcaagcgcga cggcgacctc gggctgatca agggtctcta tgccctctgc 720 ccctacattg ccggcgagtg gccgctgccg cagaacccgt cgtcgaccga gaacgagggc 780 atcctcctgc acctgcacaa caaccgcggc cgcatgacgt acggcatcga ggagttcgag 840 gcgcggaacc cgctggcgtg gcccggcttc gccactgaag acgacgtcgc cggcctcgtg 900 cccaccatca tcagcgtgaa cgagtgcgac ccgctgcgcg acgagggcgt cggcttctac 960 cgcctcctcc tccgcgcggg tgtgcccacc cggtgccgcc aggtgatggg caccatccac 1020 ggcaccgaga tcttcccgat gctgtgcccc gacgtgagcc gggacaccgc ggccagcat.c 1080 gccgacttcg cccgcaacgc cgggtccaga tctcatcacc atcaccatca ctaa 1134 Sequence Listing (2) <210> 192 <211> 377 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 192 Met Thr Leu Ile Thr Pro Ser Ser Lys Leu Thr Leu Thr Lys Gly Asn Lys Ser Trp Ser Ser Arg Ala Cys Arg Ser Thr Leu Val Asp Pro Arg Ile Asp Pro Arg Ile Lys Ala Val Met Gly Ala Phe Glu Ala Thr Ser Ala Gln Gly Asp Val Ala Ser Arg Asp Glu Leu Leu Ala Glu Ala Ala Ser Asp Glu Ala Val Ala Gln Arg Glu Met Leu Thr Ala Phe Leu Gly Met Cys Asp Thr Glu Glu Ile Ala Pro Ser Ala Gly Leu Thr Val Ser Glu His Arg Val Ala Ser Gln Pro Asp Gly Asn Lys Ile Asn Ile Arg Phe Ile Arg Pro Glu Gly Asp Asp Val Leu Pro Cys Val Tyr Tyr Ile His Gly Gly Gly Met Ala Ser Met Ser Cys Tyr Asp Gly Asn Tyr Arg Ala Trp Gly Arg Ile Ile Ala Ala Gln Gly Val Ala Val Ala Met Val Asp Phe Arg Asn Ala Leu Thr Pro Ser Ser Ala Glu Glu Val Ala Pro Sequence Listing (2) Phe Pro Ala Gly Leu Asn Asp Cys Val Ser Gly Leu Lys Trp Val Ala Ala Asn Ala Ala Asp Leu Arg Ile Asp Pro Ala Arg Ile Val Val Ala Gly Glu Ser Gly Gly Gly Asn Leu Thr Leu Ala Thr Gly Leu Lys Leu Lys Arg Asp Gly Asp Leu Gly Leu Ile Lys Gly Leu Tyr Ala Leu Cys Pro Tyr Ile Ala Gly Glu Trp Pro Leu Pro Gln Asn Pro Ser Ser Thr Glu Asn Glu Gly Ile Leu Leu His Leu His Asn Asn Arg Gly Arg Met Thr Tyr Gly Ile Glu Glu Phe Glu Ala Arg Asn Pro Leu Ala Trp Pro Gly Phe Ala Thr Glu Asp Asp Val Ala Gly Leu Val Pro Thr Ile Ile Ser Val Asn Glu Cys Asp Pro Leu Arg Asp Glu Gly Val Gly Phe Tyr Arg Leu Leu Leu Arg Ala Gly Val Pro Thr Arg Cys Arg Gin Val Met Gly Thr Ile His Gly Thr Glu Ile Phe Pro Met Leu Cys Pro Asp Val Ser Arg Asp Thr Ala Ala Ser Ile Ala Asp Phe Ala Arg Asn Ala Gly Ser Arg Ser His His His His His His <210> 193 Sequence Listing (2) <211> 1320 <212> DNA
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 193 atggaaaaat tagccgtttt atacgccgaa catattgaga cactgcaaca acgtactcgt 60 acaatttgtg aacgtgaagg gttagaggga ttagttattc actcgggcca agctaagcgt 120 cagtttttag atgatatgta ttatccattt aaagttaatc cacactttaa agcttggtta 180 ccagtactag acaatccaca ttgctggatt gtggtaaatg gtagcgacaa gcctaaactt 240 attttttatc gtccggttga tttttggcat aaagtgccag atgagccgcg cgatttttgg 300 gctgagtatt ttgacattga attgttatta cagcccgatc aggttgaaaa gctattgccc 360 tacgacaaag ctaactacgc ttatgtgggc gaatacttag aagtagcaca agcactaggc 420 tttagtgtta tgaatcctga gccggtacta aattactttc attaccaccg cgcttataaa 480 acccaatatg agcttgaatg tttacgcaac gccaaccgta ttgcagtaga tggccacaaa 540 gctgcccgcg ataccttttt taatggtggc agtgagtttg atattcagca agcttactta 600 atggcaactc gccaaagtga aaacgaaatg ccttatggca acattgtggc gctaaacgaa 660 aactgcgcca ttttgcacta cacgcacttt gaggcaaaag caccgcagca acacaactca 720 tttttaatag atgcaggcgc taatt.ttaat ggctatgcgg cagatattac ccgtacgtat 780 gactttaaaa aacaaggcga atttgcagag ctagtgcaag caatgactgc agcgcaaata 840 gagttaggta cggggttaaa acccggcatg ctttatggtg atttacatgt tgagtgtca.c 900 aatagagttg cacaaatact tagcgacttt gatattgtta agctaccggc tgaggaaata 960 gttgaacgta aaattacctc taccttcttc ccacatggtt tagggcacca cctaggttta 1020 caagtgcacg acatgggtgg ttttatggct gatgaaatgg gcgcacaaca agcagctcca 1080 gagggacatc catttttgcg ttgtactcgc ataattgaaa aaaaccaagt gtttactatt 1140 gagcctgggc tatactttat cgactctttg ctaggcgaag cacaaaccga taataaacag 1200 tttattaatt ggcaaaaggt agagtcgttt aagccttatg gtggtattcg tattgaagat 1260 aacatcatag tacacgaaga tagccttgag aatatgacac gaaatttagc gctagattaa 1320 <210> 194 Sequence Listing (2) <211> 439 <212> PRT
<213> Unknown <220>
<223> Obtained from an environmental sample <400> 194 Met Glu Lys Leu Ala Val Leu Tyr Ala Glu His Ile Glu Thr Leu Gln Gln Arg Thr Arg Thr Ile Cys Glu Arg Glu Gly Leu Glu Gly Leu Val Ile His Ser Gly Gln Ala Lys Arg Gln Phe Leu Asp Asp Met Tyr Tyr Pro Phe Lys Val Asn Pro His Phe Lys Ala Trp Leu Pro Val Leu Asp Asn Pro His Cys Trp Ile Val Val Asn Gly Ser Asp Lys Pro Lys Leu Ile Phe Tyr Arg Pro Val Asp Phe Trp His Lys Val Pro Asp Glu Pro Arg Asp Phe Trp Ala Glu Tyr Phe Asp Ile Glu Leu Leu Leu Gln Pro Asp Gln Val Glu Lys Leu Leu Pro Tyr Asp Lys Ala Asn Tyr Ala Tyr Val Gly Glu Tyr Leu Glu Val Ala Gln Ala Leu Gly Phe Ser Val Met Asn Pro Glu Pro Val Leu Asn Tyr Phe His Tyr His Arg Ala Tyr Lys Thr Gln Tyr Glu Leu Glu Cys Leu Arg Asn Ala Asn Arg Ile Ala Val Asp Gly His Lys Ala Ala Arg Asp Thr Phe Phe Asn Gly Gly Ser Glu Sequence Listing (2) Phe Asp Ile Gln Gln Ala Tyr Leu Met Ala Thr Arg Gln Ser Glu Asn Glu Met Pro Tyr Gly Asn Ile Val Ala Leu Asn Glu Asn Cys Ala Ile Leu His Tyr Thr His Phe Glu Ala Lys Ala Pro Gln Gln His Asn Ser Phe Leu Ile Asp Ala Gly Ala Asn Phe Asn Gly Tyr Ala Ala Asp Ile Thr Arg Thr Tyr Asp Phe Lys Lys Gln Gly Glu Phe Ala Glu Leu Val Gln Ala Met Thr Ala Ala Gln Ile Glu Leu Gly Thr Gly Leu Lys Pro Gly Met Leu Tyr Gly Asp Leu His Val Glu Cys His Asn Arg Val Ala Gln Ile Leu Ser Asp Phe Asp Ile Val Lys Leu Pro Ala Glu Glu Ile Val Glu Arg Lys Ile Thr Ser Thr Phe Phe Pro His Gly Leu Gly His His Leu Gly Leu Gln Val His Asp Met Gly Gly Phe Met Ala Asp Glu Met Gly Ala Gln Gln Ala Ala Pro Glu Gly His Pro Phe Leu Arg Cys Thr Arg Ile Ile Glu Lys Asn Gln Val Phe Thr Ile Glu Pro Gly Leu Tyr Phe Ile Asp Ser Leu Leu Gly Glu Ala Gln Thr Asp Asn Lys Gln Sequence Listing (2) Phe Ile Asn Trp Gln Lys Val Glu Ser Phe Lys Pro Tyr Gly Gly Ile Arg Ile Glu Asp Asn Ile Ile Val His Glu Asp Ser Leu Glu Asn Met Thr Arg Asn Leu Ala Leu Asp ti DEMANDES OU BREVETS VOLUMINEUX
LA PRESENTE PARTIE I)E CETTE DEMANDE OU CE BREVETS
COMPREND PLUS D'UN TOME.

CECI EST LE TOME a DE 2 NOTE: Pour les tomes additionels, veillez contacter le Bureau Canadien des Brevets.

JUMBO APPLICATIONS / PATENTS

THIS SECTION OF THE APPLICATION / PATENT CONTAINS MORE
THAN ONE VOLUME.

THIS IS VOLUME oZ _ OF ~2 NOTE: For additional volumes please contact the Canadian Patent Office.

Claims (235)

WHAT IS CLAIMED IS:
1. An isolated, synthetic or recombinant nucleic acid comprising (a) a nucleic acid sequence having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%, or complete sequence identity to SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ
ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID
NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID
NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID
NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:79, SEQ ID NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID
NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:101, SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ
ID NO:109, SEQ ID NO:111, SEQ ID NO:113, SEQ ID NO:115, SEQ ID NO:117, SEQ ID
NO:119, SEQ ID NO:121, SEQ ID NO:123, SEQ ID NO:125, SEQ ID NO:127, SEQ ID
NO:129, SEQ ID NO:131, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID
NO:139, SEQ ID NO:141, SEQ ID NO:143, SEQ ID NO:145, SEQ ID NO:147, SEQ ID
NO:149, SEQ ID NO:151, SEQ ID NO:153, SEQ ID NO:155, SEQ ID NO:157, SEQ ID
NO:159, SEQ ID NO:161, SEQ ID NO:163, SEQ ID NO:165, SEQ ID NO:167, SEQ ID
NO:169, SEQ ID NO:171, SEQ ID NO:173, SEQ ID NO:175, SEQ ID NO:177, SEQ ID
NO:179, SEQ ID NO:181, SEQ ID NO:183, SEQ ID NO:185, SEQ ID NO:187, SEQ ID
NO:189, SEQ ID NO:191 or SEQ ID NO:193, over a region of at least about 25, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, 1050, 1100, 1150 or more residues, wherein the nucleic acid encodes at least one polypeptide having an esterase activity, an organophosphoesterase activity, an organophosphohydrolase activity, a carboxylesterase activity, a haloperoxidase activity, a chloroperoxidase (CPO) activity, a heme-based (hCPO) or a non-heme chloroperoxidase (nhCPO) activity, a diisopropylfluorophosphatase (DFPase) activity, a dehalogenase activity, an oxidoreductase activity, a prolidase activity, an imidodipeptidase activity and/or an organophosphoric acid anhydrolase (OPAA) activity, or encodes a polypeptide or peptide capable of generating an antibody that binds specifically to a polypeptide having a sequence comprising any of the even numbered SEQ ID
NO:s in the sequence listing, including from SEQ ID NO:2 through SEQ ID NO:194, and optionally the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection;

(b) a nucleic acid sequence that hybridizes under stringent conditions to a nucleic acid comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ
ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID
NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID
NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID
NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:79, SEQ ID NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID
NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:101, SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ
ID NO:109, SEQ ID NO:111, SEQ ID NO:113, SEQ ID NO:115, SEQ ID NO:117, SEQ ID
NO:119, SEQ ID NO:121, SEQ ID NO:123, SEQ ID NO:125, SEQ ID NO:127, SEQ ID
NO:129, SEQ ID NO:131, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID
NO:139, SEQ ID NO:141, SEQ ID NO:143, SEQ ID NO:145, SEQ ID NO:147, SEQ ID
NO:149, SEQ ID NO:151, SEQ ID NO:153, SEQ ID NO:155, SEQ ID NO:157, SEQ ID
NO:159, SEQ ID NO:161, SEQ ID NO:163, SEQ ID NO:165, SEQ ID NO:167, SEQ ID
NO:169, SEQ ID NO:171, SEQ ID NO:173, SEQ ID NO:175, SEQ ID NO:177, SEQ ID
NO:179, SEQ ID NO:181, SEQ ID NO:183, SEQ ID NO:185, SEQ ID NO:187, SEQ ID
NO:189, SEQ ID NO:191 or SEQ ID NO:193, wherein the nucleic acid encodes at least one polypeptide having an esterase activity, an organophosphoesterase activity, an organophosphohydrolase activity, a carboxylesterase activity, a haloperoxidase activity, a chloroperoxidase (CPO) activity, a heme-based (hCPO) or a non-heme chloroperoxidase (nhCPO) activity, a diisopropylfluorophosphatase (DFPase) activity, a dehalogenase activity, an oxidoreductase activity, a prolidase activity, an imidodipeptidase activity and/or an organophosphoric acid anhydrolase (OPAA) activity, or encodes a polypeptide or peptide capable of generating an antibody that binds specifically to a polypeptide having a sequence comprising any of the even numbered SEQ ID
NO:s in the sequence listing, including from SEQ ID NO:2 through SEQ ID NO: 194;
and the stringent conditions include a wash step comprising a wash in 0.2X SSC
at a temperature of about 65°C for about 15 minutes, and optionally the nucleic acid is at least about 20, 30, 40, 50, 60, 75, 100, 150, 200, 225, 250, 275, 300, 350, 400, 500, 600, 700, 800, 900, 1000 or more residues in length or the full length of the gene or transcript;
(c) a nucleic acid sequence encoding a polypeptide or peptide having a sequence as set forth in 20, 30, 40, 50, 60, 75, 100, 150, 200, 225, 250, 275, 300, 350, 400 or more residues of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID
NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID
NO:44, SEQ ID NO:46, SEQ ID NO:48, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:54, SEQ ID NO:56, SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, SEQ ID
NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, SEQ ID NO:76, SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID
NO:88, SEQ ID NO:90, SEQ ID NO:92, SEQ ID NO:94, SEQ ID NO:96, SEQ ID NO:98, SEQ ID NO:100, SEQ ID NO:102, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:110, SEQ ID NO:112, SEQ ID NO:114, SEQ ID NO:116, SEQ ID NO:118, SEQ ID NO:120, SEQ ID NO:122, SEQ ID NO:124, SEQ ID NO:126, SEQ ID NO:128, SEQ ID NO:130, SEQ ID NO:132, SEQ ID NO:134, SEQ ID NO:136, SEQ ID NO:138, SEQ ID NO:140, SEQ ID NO:142, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:148, SEQ ID NO:150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NO:156, SEQ ID NO:158, SEQ ID NO:160, SEQ ID NO:162, SEQ ID NO:164, SEQ ID NO:166, SEQ ID NO:168, SEQ ID NO:170, SEQ ID NO:172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:180, SEQ ID NO:182, SEQ ID NO:184, SEQ ID NO:186, SEQ ID NO:188, SEQ ID NO:190, SEQ ID NO:192 or SEQ ID NO:194, wherein the nucleic acid encodes at least one polypeptide having an esterase activity, an organophosphoesterase activity, an organophosphohydrolase activity, a carboxylesterase activity, a haloperoxidase activity, a chloroperoxidase (CPO) activity, a heme-based (hCPO) or a non-heme chloroperoxidase (nhCPO) activity, a diisopropylfluorophosphatase (DFPase) activity, a dehalogenase activity, an oxidoreductase activity, a prolidase activity, an imidodipeptidase activity and/or an organophosphoric acid anhydrolase (OPAA) activity, or encodes a polypeptide or peptide capable of generating an antibody that binds specifically to a polypeptide having a sequence comprising any of the even numbered SEQ ID
NO:s in the sequence listing, including from SEQ ID NO:2 through SEQ ID NO:194; or (d) a nucleic acid sequence complementary to (a), (b) or (c).
2. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the nucleic acid sequence comprises a sequence as set forth in SEQ ID NO:1, SEQ ID
NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID
NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID
NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID
NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:79, SEQ ID
NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:101, SEQ ID
NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ ID NO:109, SEQ ID NO:111, SEQ ID
NO:113, SEQ ID NO:115, SEQ ID NO:117, SEQ ID NO:119, SEQ ID NO:121, SEQ ID
NO:123, SEQ ID NO:125, SEQ ID NO:127, SEQ ID NO:129, SEQ ID NO:131, SEQ ID
NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:139 SEQ ID NO:141, SEQ ID
NO:143, SEQ ID NO:145, SEQ ID NO:147, SEQ ID NO:149, SEQ ID NO:151, SEQ ID
NO:153, SEQ ID NO:155, SEQ ID NO:157, SEQ ID NO:159, SEQ ID NO:161, SEQ ID
NO:163, SEQ ID NO:165, SEQ ID NO:167, SEQ ID NO:169, SEQ ID NO:171, SEQ ID
NO:173, SEQ ID NO:175, SEQ ID NO:177, SEQ ID NO:179, SEQ ID NO:181, SEQ ID
NO:183, SEQ ID NO:185, SEQ ID NO:187, SEQ ID NO:189, SEQ ID NO:191 or SEQ ID
NO:193.
3. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the sequence comparison algorithm comprises a BLAST version 2.2.2 algorithm where a filtering setting is set to blastall -p blastp -d "nr pataa" -F F, and all other options are set to default.
4. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a detoxifying or decontaminating activity.
5. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a V agent.
6. The isolated, synthetic or recombinant nucleic acid of claim 5, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a V agent, or the enzymatic activity comprises a haloperoxidase activity, or an activity comprising catalyzing the hydrolysis of a methylphosphonofluoridate or a thiophosphoric ester, or a combination thereof.
7. The isolated, synthetic or recombinant nucleic acid of claim 6, wherein the haloperoxidase activity comprises a chloroperoxidase activity.
8. The isolated, synthetic or recombinant nucleic acid of claim 5, wherein the V
agent comprises VX (O-Ethyl-S-[2(diisopropylamino)ethyl]
methylphosphonothioate, or methylphosphonothioic acid), VE (O-Ethyl-S-[2-(diethylamino)ethyl]
ethylphosphonothioate), VG (O,O-Diethyl-S-[2-(diethylamino)ethyl]
phosphorothioate), VM
(O-Ethyl-S-[2-(diethylamino)ethyl] methylphosphonothioate), VR
(Phosphonothioic acid) Soviet V-gas (Russian VX), Tetriso (O,O-diisopropyl S-(2-diisopropylaminoethyl) phosphorothiolate) or a phosphorylthiocholine-comprising compound.
9. The isolated, synthetic or recombinant nucleic acid of claim 5, wherein the enzyme hydrolyzing or decontaminating a V agent comprises a polypeptide encoded by a nucleic having a sequence as set forth in SEQ ID NO:1, SEQ ID NO:75; SEQ ID
NO:77;
SEQ ID NO: 89; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:127; SEQ ID NO:151;
SEQ ID NO:167; SEQ ID NO:171; SEQ ID NO:187 or SEQ ID NO:191, or having an amino acid sequence as set forth in SEQ ID NO:2, SEQ ID NO:76; SEQ ID NO:78; SEQ ID
NO:90;

SEQ ID NO:118; SEQ ID NO:120; SEQ ID NO:128; SEQ ID NO:152; SEQ ID NO:168;
SEQ ID NO:172; SEQ ID NO:189 or SEQ ID NO:192.
10. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a G agent.
11. The isolated, synthetic or recombinant nucleic acid of claim 10, wherein the enzymatic activity comprising hydrolysis of, or decontamination of, a G agent comprises an organophosphoric acid anhydrolase (OPAA) activity.
12. The isolated, synthetic or recombinant nucleic acid of claim 10, wherein the G
agent comprises tabun (GA), sarin (methylphosphonofluoridic acid) (GB), soman (GD), cyclosarin (GF) or a combination thereof.
13. The isolated, synthetic or recombinant nucleic acid of claim 5, wherein the enzyme hydrolyzing or decontaminating a G agent comprises a polypeptide encoded by a nucleic having a sequence as set forth in SEQ ID NO:73; SEQ ID NO:93; SEQ ID
NO:95;
SEQ ID NO:97; SEQ ID NO:99; SEQ ID NO:101; SEQ ID NO:103; SEQ ID NO:105; SEQ
ID NO:107; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:113; SEQ ID NO:115; SEQ ID
NO:117; SEQ ID NO:119; SEQ ID NO:121; SEQ ID NO:123; SEQ ID NO:125; SEQ ID
NO:127; SEQ ID NO:129; SEQ ID NO:131; SEQ ID NO:133; SEQ ID NO:135; SEQ ID
NO:137; SEQ ID NO:139; SEQ ID NO:141; SEQ ID NO:143; SEQ ID NO:145; SEQ ID
NO:147; SEQ ID NO:149; SEQ ID NO:151; SEQ ID NO:153; SEQ ID NO:155; SEQ ID
NO:157; SEQ ID NO:159; SEQ ID NO:161; SEQ ID NO:163; SEQ ID NO:165; SEQ ID
NO:167; SEQ ID NO:169; SEQ ID NO:171; SEQ ID NO:173; SEQ ID NO:175; SEQ ID
NO:177; SEQ ID NO:179; SEQ ID NO:181; SEQ ID NO:183; SEQ ID NO:185; SEQ ID
NO:187 or SEQ ID NO:191; or, a polypeptide having a prolidase activity or an organophosphoric acid anhydrolase (OPAA) activity and encoded by a nucleic having a sequence as set forth in SEQ ID NO:193, or having a sequence as set forth in SEQ ID
NO: 194.
14. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, an H agent.
15. The isolated, synthetic or recombinant nucleic acid of claim 14, wherein the H
agent comprises a chloroperoxidase activity, a dehalogenase activity or a combination thereof.
16. The isolated, synthetic or recombinant nucleic acid of claim 14, wherein the H
agent comprises mustard gas; 1 1' thiobis [2 chloroethane] bis-(2-chloroethyl) sulphide; beta, beta' dichloroethyl sulphide; 2, 2' dichloroethyl sulphide; bis (beta-chloroethyl) sulphide; 1-chloro-2 (beta-chlorodiethylthio) ethane; or, a combination thereof.
17. The isolated, synthetic or recombinant nucleic acid of claim 14, wherein the enzyme hydrolyzing or decontaminating an H agent comprises a polypeptide encoded by a nucleic acid encoding a chloroperoxidase and having a sequence as set forth in SEQ ID
NO:1; SEQ ID NO:53; SEQ ID NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61;
SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or the enzyme has an amino acid sequence as set forth in SEQ ID NO:2; SEQ ID NO:54; SEQ ID NO:56; SEQ ID
NO:58;
SEQ ID NO:60; SEQ ID NO:62; SEQ ID NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively, or a nucleic acid encoding a dehalogenase and having a sequence as set forth in SEQ
ID NO:69 or SEQ ID NO:91 or the enzyme has an amino acid sequence as set forth in SEQ
ID NO:70 or SEQ ID NO:92..
18. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a biological agen.t.
19. The isolated, synthetic or recombinant nucleic acid of claim 18, wherein the enzymatic activity comprises a chloroperoxidase activity.
20. The isolated, synthetic or recombinant nucleic acid of claim 18, wherein the biological agent is a gram negative spore.
21. The isolated, synthetic or recombinant nucleic acid of claim 20, wherein the gram negative spore comprises an anthrax, or Bacillus anthracis, spore.
22. The isolated, synthetic or recombinant nucleic acid of claim 18 wherein the enzyme hydrolyzing or decontaminating a biological agent comprises a polypeptide encoded by a nucleic having a sequence as set forth in SEQ ID NO:1, SEQ ID NO:53; SEQ
ID
NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID
NO:2;
SEQ ID NO:54; SEQ ID NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID NO:62; SEQ ID
NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively.
23. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a P-F bond.
24. The isolated, synthetic or recombinant nucleic acid of claim 23, wherein the polypeptide having P-F bond hydrolyzing or decontamination activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:71; SEQ ID NO:73; SEQ ID
NO:93;
SEQ ID NO:95; SEQ ID NO:97; SEQ ID NO:99; SEQ ID NO: 101; SEQ ID NO:103; SEQ
ID NO:105; SEQ ID NO:107; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:113; SEQ ID
NO:115; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:121; SEQ ID NO:123; SEQ ID
NO:125; SEQ ID NO:127; SEQ ID NO:129; SEQ ID NO:131; SEQ ID NO:133; SEQ ID
NO:135; SEQ ID NO:137; SEQ ID NO:139; SEQ ID NO:141; SEQ ID NO:143; SEQ ID
NO:145; SEQ ID NO:147; SEQ ID NO:149; SEQ ID NO:151; SEQ ID NO:153; SEQ ID
NO:155; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:161; SEQ ID NO:163; SEQ ID
NO:165; SEQ ID NO:167; SEQ ID NO:169; SEQ ID NO:171; SEQ ID NO:173; SEQ ID
NO:175; SEQ ID NO:177; SEQ ID NO:179; SEQ ID NO:181; SEQ ID NO:183; SEQ ID
NO:185; SEQ ID NO:187, SEQ ID NO:191 or SEQ ID NO:193, or has an amino acid sequence as set forth in SEQ ID NO:72; SEQ ID NO:74; SEQ ID NO:94; SEQ ID
NO:96;
SEQ ID NO:98; SEQ ID NO:100; SEQ ID NO:102; SEQ ID NO:104; SEQ ID NO:106; SEQ
ID NO:108; SEQ ID NO:110; SEQ ID NO:112; SEQ ID NO:114; SEQ ID NO:116; SEQ ID
NO:118; SEQ ID NO:120; SEQ ID NO:122; SEQ ID NO:124; SEQ ID NO:126; SEQ ID

NO:128; SEQ ID NO:130; SEQ ID NO:132; SEQ ID NO:134; SEQ ID NO:136; SEQ ID
NO:138; SEQ ID NO:140; SEQ ID NO:142; SEQ ID NO:144; SEQ ID NO:146; SEQ ID
NO:148; SEQ ID NO:150; SEQ ID NO:152; SEQ ID NO:154; SEQ ID NO:156; SEQ ID
NO:158; SEQ ID NO:160; SEQ ID NO:162; SEQ ID NO:164; SEQ ID NO:166; SEQ ID
NO:168; SEQ ID NO:170; SEQ ID NO:172; SEQ ID NO:174; SEQ ID NO:176; SEQ ID
NO:178; SEQ ID NO:180; SEQ ID NO:182; SEQ ID NO:184; SEQ ID NO:186; SEQ ID
NO:188, SEQ ID NO:192 or SEQ ID NO:194.
25. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a P-S bond.
26. The isolated, synthetic or recombinant nucleic acid of claim 25, wherein the polypeptide having P-S bond hydrolyzing or decontamination activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID NO: 75; SEQ ID
NO: 77;
SEQ ID NO: 89; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:127; SEQ ID NO:151;
SEQ ID NO:167; SEQ ID NO:171; SEQ ID NO:187 or SEQ ID NO:191, or has an amino acid sequence as set forth in SEQ ID NO:2; SEQ ID NO:76; SEQ ID NO: 78; SEQ ID

NO:90; SEQ ID NO:118; SEQ ID NO:120; SEQ ID NO:128; SEQ ID NO:152; SEQ ID
NO:168; SEQ ID NO:172; SEQ ID NO:188 or SEQ ID NO:192.
27. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises an organophosphoric acid anhydrolase (OPAA) activity.
28. The isolated, synthetic or recombinant nucleic acid of claim 27, wherein the enzyme having organophosphoric acid anhydrolase (OPAA) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:193, or has an amino acid sequence as set forth in SEQ ID NO:194.
29. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises an aminopeptidase activity.
30. The isolated, synthetic or recombinant nucleic acid of claim 27, wherein the enzyme having aminopeptidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:189, or has an amino acid sequence as set forth in SEQ ID
NO:190.
31. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a carboxylesterase activity.
32. The isolated, synthetic or recombinant nucleic acid of claim 27, wherein the enzyme having carboxylesterase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:73; SEQ ID NO:75; SEQ ID NO:77; SEQ ID NO:79; SEQ ID NO:81;

SEQ ID NO:83; SEQ ID NO:85; SEQ ID NO:87 or SEQ ID NO:89.
33. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a heme-based peroxidase activity.
34. The isolated, synthetic or recombinant nucleic acid of claim 33, wherein the enzyme having heme-based peroxidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:3; SEQ ID NO:5; SEQ ID NO:7; SEQ ID NO:9; SEQ ID NO:11;

SEQ ID NO:13; SEQ ID NO:15; SEQ ID NO:17; SEQ ID NO:19; SEQ ID NO:21; SEQ ID
NO:23; SEQ ID NO:25; SEQ ID NO:27; SEQ ID NO:29; SEQ ID NO:31; SEQ ID NO:33;
SEQ ID NO:35; SEQ ID NO:37; SEQ ID NO:39; SEQ ID NO:41; SEQ ID NO:43; SEQ ID
NO:45; SEQ ID NO:47; SEQ ID NO:49 or SEQ ID NO:51, or, the enzyme has a sequence as set forth in SEQ ID NO:4; SEQ ID NO:6; SEQ ID NO:8; SEQ ID NO:10; SEQ ID
NO:12;
SEQ ID NO:14; SEQ ID NO:16; SEQ ID NO:18; SEQ ID NO:20; SEQ ID NO:22; SEQ ID
NO:24; SEQ ID NO:26; SEQ ID NO:28; SEQ ID NO:30; SEQ ID NO:32; SEQ ID NO:34;
SEQ ID NO:36; SEQ ID NO:38; SEQ ID NO:40; SEQ ID NO:42; SEQ ID NO:44; SEQ ID
NO:46; SEQ ID NO:48; SEQ ID NO:50 or SEQ ID NO:52, respectively.
35. The isolated, synthetic or recombinant nucleic acid of claim 34, wherein the enzyme has a heme-based chloroperoxidase activity and is encoded by a nucleic having a sequence as set forth in SEQ ID NO:1, or has an amino acid sequence as set forth in SEQ ID
NO:2.
36. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a non-heme-based peroxidase activity.
37. The isolated, synthetic or recombinant nucleic acid of claim 37, wherein the enzyme having non-heme-based chloroperoxidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID NO:53; SEQ ID NO:55; SEQ ID
NO:57;
SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:2; SEQ ID NO:54;
SEQ ID
NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID NO:62; SEQ ID NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively.
38. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a dehalogenase activity.
39. The isolated, synthetic or recombinant nucleic acid of claim 38, wherein the enzyme having dehalogenase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:69 or SEQ ID NO:91, or has an amino acid sequence as set forth in SEQ ID
NO:70 or SEQ ID NO:92, respectively.
40. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a diisopropylfluorophosphatase (DFPase) activity.
41. The isolated, synthetic or recombinant nucleic acid of claim 38, wherein the enzyme having diisopropylfluorophosphatase (DFPase) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:71, or has an amino acid sequence as set forth in SEQ ID NO:72.
42. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises an organophosphoesterase and can hydrolyze a P-S
or a P-F
bond and detoxify an acetylcholinesterase- or butyrylcholinesterase-inhibitor.
43. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises a halogenation reaction to form a hypohalite from hydrogen peroxide and chloride, bromide or iodide.
44. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises catalysis of the transfer of oxygen from hydrogen peroxide to an organic substrate.
45. The isolated, synthetic or recombinant nucleic acid of claim 44, wherein the enzymatic activity comprises chemical bleaching of lignin.
46. The isolated, synthetic or recombinant nucleic acid of claim 45, wherein the enzymatic activity comprises chemical bleaching of lignin in a pulp or paper manufacturing process.
47. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the enzymatic activity comprises detoxifying a pesticide, herbicide and/or insecticide.
48. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the pesticide is Demeton-S, Demeton-S-methyl, Demeton-S-methylsulphon, Demeton-methyl, Parathion, Phosmet, Carbophenothion, Benoxafos, Azinphos-methyl, Azinphos-ethyl, Amiton, Amidithion, Cyanthoate, Dialiphos, Dimethoate, Dioxathion, Disulfoton, Endothion, Etion, Ethoate-methyl, Formothion, Malathion, Mercarbam, Omethoate, Oxydeprofos, Oxydisulfoton, Phenkapton, Phorate, Phosalone, Prothidathion, Prothoate, Sophamide, Thiometon, Vamidothion, Methamidophos or a combination thereof.
49. The isolated, synthetic or recombinant nucleic acid of any one of claims 1 to 48, wherein the nucleic acid encodes a thermostable or thermotolerant enzyme or protein.
50. The isolated, synthetic or recombinant nucleic acid of claim 49, wherein the enzyme or protein retains activity under conditions comprising a temperature range of between about 37°C to about 95°C, or between about 55°C
to about 85°C, or between about 70°C to about 75°C, or between about 70°C to about 95°C, or between about 90°C to about 95°C, or, retains enzyme activity under conditions comprising a temperature range of from greater than 37°C to about 95°C, from greater than 55°C
to about 85°C, or between about 70°C to about 75°C, or from greater than 90°C to about 95°C, or in the range between about 1°C to about 5°C, between about 5°C to about 15°C, between about 15°C to about 25°C, between about 25°C to about 37°C, between about 37°C to about 95°C.
51. The isolated, synthetic or recombinant nucleic acid of claim 49, wherein the enzyme or protein retains an activity after exposure to a temperature in the range from greater than 37°C to about 95°C, from greater than 55°C
to about 85°C, from greater than 90°C to about 95°C, or after exposure to a temperature in the range between about 1°C to about 5°C, between about 5°C to about 15°C, between about 15°C to about 25°C, between about 25°C to about 37°C, between about 37°C to about 95°C, between about 55°C to about 85°C, between about 70°C to about 75°C, or between about 90°C to about 95°C.
52. A nucleic acid probe for identifying a nucleic acid encoding a polypeptide with a hydrolase activity, an esterase activity, an organophosphohydrolase activity, an organophosphoesterase activity, a carboxylesterase activity, a haloperoxidase activity, a heme-based (hCPO) or a non-heme chloroperoxidase (nhCPO) activity, a diisopropylfluorophosphatase (DFPase) activity, a dehalogenase activity, an oxidoreductase activity, a prolidase activity, an imidodipeptidase activity or an organophosphoric acid anhydrolase (OPAA) activity, wherein the probe comprises at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 150, 200, 250 or more consecutive bases of:

(a) a sequence as set forth in claim 1, or (b) a nucleic acid comprising at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 150, 200, 250 or more consecutive residues of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID
NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID
NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID
NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID
NO:79, SEQ ID NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID
NO:101, SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ ID NO:109, SEQ ID
NO:111, SEQ ID NO:113, SEQ ID NO:115, SEQ ID NO:117, SEQ ID NO:119, SEQ ID
NO:121, SEQ ID NO:123, SEQ ID NO:125, SEQ ID NO:127, SEQ ID NO:129, SEQ ID
NO:131, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:139, SEQ ID
NO:141, SEQ ID NO:143, SEQ ID NO:145, SEQ ID NO:147, SEQ ID NO:149, SEQ ID
NO:151, SEQ ID NO:153, SEQ ID NO:155, SEQ ID NO:157, SEQ ID NO:159, SEQ ID
NO:161, SEQ ID NO:163, SEQ ID NO:165, SEQ ID NO:167, SEQ ID NO:169, SEQ ID
NO:171, SEQ ID NO:173, SEQ ID NO:175, SEQ ID NO:177, SEQ ID NO:179, SEQ ID
NO:181, SEQ ID NO:183, SEQ ID NO:185, SEQ ID NO:187, SEQ ID NO:189, SEQ ID
NO:191 or SEQ ID NO:193;
wherein the probe identifies the nucleic acid by binding or hybridization.
53. The nucleic acid probe of claim 52, wherein the probe comprises an oligonucleotide comprising at least about 10 to 50, about 20 to 60, about 30 to 70, about 40 to 80, about 60 to 100, or about 50 to 150 consecutive bases.
54. An amplification primer pair for amplifying a nucleic acid encoding a polypeptide having an enzymatic activity or encoding a protein, wherein the primer pair is capable of amplifying a nucleic acid comprising (a) a sequence as set forth in claim 1, or a subsequence thereof; or (b) a nucleic acid comprising at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 150, 200, 250 or more consecutive residues of SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID
NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID
NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID
NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID

NO:79, SEQ ID NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID
NO:101, SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ ID NO:109, SEQ ID
NO:111, SEQ ID NO:113, SEQ ID NO:115, SEQ ID NO:117, SEQ ID NO:119, SEQ ID
NO:121, SEQ ID NO:123, SEQ ID NO:125, SEQ ID NO:127, SEQ ID NO:129, SEQ ID
NO:131, SEQ ID NO:133, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:139, SEQ ID
NO:141, SEQ ID NO:143, SEQ ID NO:145, SEQ ID NO:147, SEQ ID NO:149, SEQ ID
NO:151, SEQ ID NO:153, SEQ ID NO:155, SEQ ID NO:157, SEQ ID NO:159, SEQ ID
NO:161, SEQ ID NO:163, SEQ ID NO:165, SEQ ID NO:167, SEQ ID NO:169, SEQ ID
NO:171, SEQ ID NO:173, SEQ ID NO:175, SEQ ID NO:177, SEQ ID NO:179, SEQ ID
NO:181, SEQ ID NO:183, SEQ ID NO:185, SEQ ID NO:187, SEQ ID NO:189, SEQ ID
NO:191 or SEQ ID NO:193, wherein a member of the amplification primer pair comprises an oligonucleotide comprising at least about 10 to 50 consecutive bases of the sequence, or, about 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40 or more consecutive bases of the sequence.
55. The amplification primer pair of claim 54, wherein the amplification primer pair amplifies a nucleic acid encoding a polypeptide with a hydrolase activity, an esterase activity, an organophosphohydrolase activity, an organophosphoesterase activity, a carboxylesterase activity, a haloperoxidase activity, a heme-based (hCPO) or a non-heme chloroperoxidase (nhCPO) activity, a diisopropylfluorophosphatase (DFPase) activity, a dehalogenase activity, an oxidoreductase activity, a prolidase activity, an imidodipeptidase activity or an organophosphoric acid anhydrolase (OPAA) activity.
56. The amplification primer pair of claim 54, comprising a first member having a sequence as set forth by about the first (the 5') 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 42, 33, 34, 35 or more residues of the nucleic acid of (a) or (b), and a second member having a sequence as set forth by about the first (the 5') 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 42, 33, 34, 35 or more residues of the complementary strand of the first member.
57. An enzyme-encoding or protein-encoding nucleic acid generated by amplification of a polynucleotide using an amplification primer pair as set forth in any of claims 54 to 56.
58. The enzyme-encoding or protein-encoding nucleic acid of claim 57, wherein the amplification is by a polymerase chain reaction (PCR).
59. The enzyme-encoding or protein-encoding nucleic acid of claim 57, wherein the nucleic acid is generated by amplification of a gene library, and optionally the gene library is an environmental library.
60. An isolated, synthetic or recombinant polypeptide having an enzymatic activity or encoding a protein encoded by a nucleic acid having a sequence as set forth in claim 57.
61. A method of amplifying a nucleic acid encoding a polypeptide having an enzymatic activity or encoding a protein comprising amplification of a template nucleic acid with an amplification primer pair as set forth in any of claims 54 to 56, wherein the amplification primer pair is capable of amplifying a nucleic acid sequence as set forth in claim 1, or a subsequence thereof.
62. An expression cassette comprising a nucleic acid comprising a sequence as set forth in claim 1 or claim 57.
63. A vector comprising a nucleic acid comprising a sequence as set forth in claim 1 or claim 57.
64. A cloning vehicle comprising a nucleic acid comprising a sequence as set forth in claim 1 or claim 57, wherein the cloning vehicle comprises a viral vector, a plasmid, a phage, a phagemid, a cosmid, a fosmid, a bacteriophage or an artificial chromosome.
65. The cloning vehicle of claim 64, wherein the viral vector comprises an adenovirus vector, a retroviral vector or an adeno-associated viral vector.
66. A bacterial artificial chromosome (BAC), a bacteriophage P 1-derived vector (PAC), a yeast artificial chromosome (YAC) or a mammalian artificial chromosome (MAC) comprising a sequence as set forth in claim 1 or claim 57.
67. A transformed cell comprising a nucleic acid comprising: a sequence as set forth in claim 1 or claim 57; or, an expression cassette as set forth in claim 62, a cloning vehicle as set forth in claim 64, or a bacterial artificial chromosome (BAC), a bacteriophage P1-derived vector (PAC), a yeast artificial chromosome (YAC) or a mammalian artificial chromosome (MAC) as set forth in claim 66.
68. The transformed cell of claim 67, wherein the cell is a bacterial cell, a mammalian cell, a fungal cell, a yeast cell, an insect cell or a plant cell.
69. A transgenic non-human animal comprising a sequence as set forth in claim 1 or claim 57, and optionally the animal is a mouse, a goat, a rabbit, a sheep, a pig, a cow or a rat.
70. A transgenic plant comprising a sequence as set forth in claim 1 or claim 57, and optionally the plant is a corn plant, a sorghum plant, a potato plant, a tomato plant, a wheat plant, an oilseed plant, a rapeseed plant, a soybean plant, a rice plant, a barley plant, a grass, or a tobacco plant.
71. A transgenic seed comprising a sequence as set forth in claim 1 or claim 57, and optionally the seed is a corn seed, a wheat kernel, an oilseed, a rapeseed, a soybean seed, a palm kernel, a sunflower seed, a sesame seed, a rice, a barley, a peanut or a tobacco plant seed.
72. An antisense oligonucleotide comprising a nucleic acid sequence complementary to or capable of hybridizing under stringent conditions to a sequence as set forth in claim 1 or claim 57, or a subsequence thereof, and optionally the antisense oligonucleotide has a length of between about 10 to 50, about 20 to 60, about 30 to 70, about 40 to 80, or about 60 to 100 bases in length.
73. A method of inhibiting the translation of an enzyme-encoding or protein-encoding message in a cell comprising administering to the cell or expressing in the cell an antisense oligonucleotide comprising a nucleic acid sequence complementary to or capable of hybridizing under stringent conditions to a sequence as set forth in claim 1 or claim 57.
74. A double-stranded inhibitory RNA (RNAi) molecule comprising a subsequence of a sequence as set forth in claim 1 or claim 57, wherein optionally the RNAi is an siRNA or an miRNA molecule, or optionally the RNAi is about 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or more duplex nucleotides in length, wherein optionally the RNAi is an siRNA or an miRNA molecule.
75. A method of inhibiting the expression of an enzyme or a protein in a cell comprising administering to the cell or expressing in the cell a double-stranded inhibitory RNA (iRNA) as set forth in claim 74.
76. An isolated, synthetic or recombinant polypeptide or peptide:
(a) having at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90'%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more or complete sequence identity to SEQ
ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID
NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:34, SEQ ID NO:36, SEQ ID NO:38, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, SEQ ID
NO:46, SEQ ID NO:48, SEQ ID NO:50, SEQ ID NO:52, SEQ ID NO:54, SEQ ID NO:56, SEQ ID NO:58, SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:64, SEQ ID NO:66, SEQ ID
NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, SEQ ID NO:76, SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID NO:88, SEQ ID
NO:90, SEQ ID NO:92, SEQ ID NO:94, SEQ ID NO:96, SEQ ID NO:98, SEQ ID NO:100, SEQ ID NO:102, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:110, SEQ ID NO:112, SEQ ID NO:114, SEQ ID NO:116, SEQ ID NO:118, SEQ ID NO:120, SEQ ID NO:122, SEQ ID NO:124, SEQ ID NO:126, SEQ ID NO:128, SEQ ID NO:130, SEQ ID NO:132, SEQ ID NO:134, SEQ ID NO:136, SEQ ID NO:138, SEQ ID NO:140, SEQ ID NO:142, SEQ ID NO:143, SEQ ID NO:146, SEQ ID NO:148, SEQ ID NO:150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NO:156, SEQ ID NO:158, SEQ ID NO:160, SEQ ID NO:162, SEQ ID NO:164, SEQ ID NO:166, SEQ ID NO:168, SEQ ID NO:170, SEQ ID NO:172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:180, SEQ ID NO:182, SEQ ID NO:184, SEQ ID NO:186, SEQ ID NO:188, SEQ ID NO:190, SEQ ID NO:192 or SEQ ID NO:194, over a region of at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 75, 80, 90, 100, 125, 150, 175, 200, 250, 300, 350, 400, 450, 500, 550, 00, 650, 700 or more residues, wherein optionally the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection, or, (ii) encoded by a nucleic acid as set forth in claim 1;
wherein the polypeptide or peptide of (i) or (ii) has an esterase activity, an organophosphoesterase activity, an organophosphohydrolase activity, a carboxylesterase activity, a haloperoxidase activity, a chloroperoxidase (CPO) activity, a heme-based (hCPO) or a non-heme chloroperoxidase (nhCPO) activity, a diisopropylfluorophosphatase (DFPase) activity, a dehalogenase activity, an oxidoreductase activity, a prolidase activity, an imidodipeptidase activity and/or an organophosphoric acid anhydrolase (OPAA) activity, or encodes a polypeptide or peptide capable of generating an antibody that binds specifically to a polypeptide having a sequence comprising any of the even numbered SEQ ID
NO:s in the sequence listing, including from SEQ ID NO:2 through SEQ ID NO: 194.
77. The isolated, synthetic or recombinant nucleic acid of claim 1, wherein the sequence comparison algorithm comprises a BLAST version 2.2.2 algorithm where a filtering setting is set to blastall -p blastp -d "nr pataa" -F F, and all other options are set to default.
78. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a detoxifying or decontaminating activity.
79. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a V agent.
80. The isolated, synthetic or recombinant polypeptide or peptide of claim 79, wherein the enzymatic activity comprising hydrolysis of, or decontamination of, a V agent comprises a haloperoxidase activity, an activity comprising catalyzing the hydrolysis of a methylphosphonofluoridate or a thiophosphoric ester, or a combination thereof.
81. The isolated, synthetic or recombinant polypeptide or peptide of claim 80, wherein the haloperoxidase activity comprises a chloroperoxidase activity.
82. The isolated, synthetic or recombinant polypeptide or peptide of claim 79, wherein the V agent comprises VX(O-Ethyl-S-[2(diisopropylamino)ethyl]
methylphosphonothioate, or methylphosphonothioic acid), VE(O-Ethyl-S-[2-(diethylamino)ethyl] ethylphosphonothioate), VG(O,O-Diethyl-S-[2-(diethylamino)ethyl]
phosphorothioate), VM(O-Ethyl-S-[2-(diethylamino)ethyl]methylphosphonothioate), VR
(Phosphonothioic acid) Soviet V-gas (Russian VX), Tetriso (O,O-diisopropyl S-(2-diisopropylaminoethyl) phosphorothiolate) or a phosphorylthiocholine-comprising compound.
83. The isolated, synthetic or recombinant polypeptide or peptide of claim 79, wherein the enzyme hydrolyzing or decontaminating a V agent comprises a polypeptide encoded by a nucleic having a sequence as set forth in SEQ ID NO: 75; SEQ ID
NO: 77;
SEQ ID NO: 89; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:127; SEQ ID NO:151;
SEQ ID NO:167; SEQ ID NO:171; SEQ ID NO:187 or SEQ ID NO:191.
84. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a G agent.
85. The isolated, synthetic or recombinant polypeptide or peptide of claim 84, wherein the enzymatic activity comprising hydrolysis of, or decontamination of, a G agent comprises an organophosphoric acid anhydrolase (OPAA) activity, or, diisopropylfluorophosphatase (DFPase) activity.
86. The isolated, synthetic or recombinant polypeptide or peptide of claim 84, wherein the G agent comprises tabun (GA), sarin (methylphosphonofluoridic acid) (GB), soman (GD), cyclosarin (GF) or a combination thereof.
87. The isolated, synthetic or recombinant polypeptide or peptide of claim 84, wherein the enzyme hydrolyzing or decontaminating a G agent comprises a polypeptide encoded by a nucleic having a sequence as set forth in SEQ ID NO:71; SEQ ID
NO:7:3; SEQ
ID NO:93; SEQ ID NO:95; SEQ ID NO:97; SEQ ID NO:99; SEQ ID NO:101; SEQ ID
NO:103; SEQ ID NO:105; SEQ ID NO:107; SEQ ID NO:109; SEQ ID NO:111; SEQ ID
NO:113; SEQ ID NO:115; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:121; SEQ ID
NO:123; SEQ ID NO:125; SEQ ID NO:127; SEQ ID NO:129; SEQ ID NO:131; SEQ ID
NO:133; SEQ ID NO:135; SEQ ID NO:137; SEQ ID NO:139; SEQ ID NO:141; SEQ ID
NO:143; SEQ ID NO:145; SEQ ID NO:147; SEQ ID NO:149; SEQ ID NO:151; SEQ ID
NO:153; SEQ ID NO:155; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:161; SEQ ID
NO:163; SEQ ID NO:165; SEQ ID NO:167; SEQ ID NO:169; SEQ ID NO:171; SEQ ID
NO:173; SEQ ID NO:175; SEQ ID NO:177; SEQ ID NO:179; SEQ ID NO:181; SEQ ID
NO:183; SEQ ID NO:185; SEQ ID NO:187, or SEQ ID NO:191; or has an amino acid sequence as set forth in SEQ ID NO:72; SEQ ID NO:74; SEQ ID NO:94; SEQ ID
NO:96;
SEQ ID NO:98; SEQ ID NO:100; SEQ ID NO:102; SEQ ID NO:104; SEQ ID NO:106; SEQ
ID NO:108; SEQ ID NO:110; SEQ ID NO:112; SEQ ID NO:114; SEQ ID NO:116; SEQ ID
NO:118; SEQ ID NO:120; SEQ ID NO:122; SEQ ID NO:124; SEQ ID NO:126; SEQ ID
NO:128; SEQ ID NO:130; SEQ ID NO:132; SEQ ID NO:134; SEQ ID NO:136; SEQ ID
NO:138; SEQ ID NO:140; SEQ ID NO:142; SEQ ID NO:144; SEQ ID NO:146; SEQ ID
NO:148; SEQ ID NO:150; SEQ ID NO:152; SEQ ID NO:154; SEQ ID NO:156; SEQ ID
NO:158; SEQ ID NO:160; SEQ ID NO:162; SEQ ID NO:164; SEQ ID NO:166; SEQ ID
NO:168; SEQ ID NO:170; SEQ ID NO:172; SEQ ID NO:174; SEQ ID NO:176; SEQ ID
NO:178; SEQ ID NO:180; SEQ ID NO:182; SEQ ID NO:184; SEQ ID NO:186; SEQ ID
NO:188, or SEQ ID NO:192, or, a polypeptide having a prolidase activity or an organophosphoric acid anhydrolase (OPAA) activity and encoded by a nucleic having a sequence as set forth in SEQ
ID NO: 193, or has an amino acid sequence as set forth in SEQ ID NO: 194; or, a polypeptide having diisopropyl-fluorophosphatase (DFPase) activity encoded by a nucleic having a sequence as set forth in SEQ ID NO:71, or has an amino acid sequence as set forth in SEQ
ID NO:72.
88. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, an H agent.
89. The isolated, synthetic or recombinant polypeptide or peptide of claim 88, wherein the H agent comprises a chloroperoxidase activity, a dehalogenase activity or a combination thereof.
90. The isolated, synthetic or recombinant polypeptide or peptide of claim 88, wherein the H agent comprises mustard gas; 1 1' thiobis [2 chloroethane] bis-(2-chloroethyl) sulphide; beta, beta' dichloroethyl sulphide; 2, 2' dichloroethyl sulphide;
bis (beta-chloroethyl) sulphide; 1-chloro-2 (beta-chlorodiethylthio) ethane; or, a combination thereof.
91. The isolated, synthetic or recombinant polypeptide or peptide of claim 88, wherein the enzyme hydrolyzing or decontaminating an H agent comprises a polypeptide encoded by a nucleic acid encoding a chloroperoxidase and having a sequence as set forth in SEQ ID NO:1; SEQ ID NO:53; SEQ ID NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID
NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or the enzyme has an amino acid sequence as set forth in SEQ ID NO:2; SEQ ID NO:54; SEQ ID NO:56; SEQ ID
NO:58;
SEQ ID NO:60; SEQ ID NO:62; SEQ ID NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively, or a nucleic acid encoding a dehalogenase and having a sequence as set forth in SEQ ID NO:69 or SEQ ID NO:91, or has an amino acid sequence as set forth in SEQ ID
NO:70 or SEQ ID NO:92, respectively.
92. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a biological agent.
93. The isolated, synthetic or recombinant polypeptide or peptide of claim 92, wherein the enzymatic activity comprises a haloperoxidase activity.
94. The isolated, synthetic or recombinant polypeptide or peptide of claim 93, wherein the haloperoxidase activity comprises a chloroperoxidase activity.
95. The isolated, synthetic or recombinant polypeptide or peptide of claim 92, wherein the biological agent is a gram negative spore.
96. The isolated, synthetic or recombinant polypeptide or peptide of claim 94, wherein the gram negative spore comprises an anthrax, or Bacillus anthracis, spore.
97. The isolated, synthetic or recombinant polypeptide or peptide of claim 92, wherein the enzyme hydrolyzing or decontaminating a biological agent comprises a polypeptide encoded by a nucleic having a sequence as set forth in SEQ ID
NO:1; SEQ ID
NO:53; SEQ ID NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63;
SEQ ID NO:65 or SEQ ID NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:2; SEQ ID NO:54; SEQ ID NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID
NO:62; SEQ ID NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively.
98. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a P-F bond.
99. The isolated, synthetic or recombinant polypeptide or peptide of claim 98, wherein the polypeptide having P-F bond hydrolyzing or decontamination activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:73; SEQ ID NO:93; SEQ
ID
NO:95; SEQ ID NO:97; SEQ ID NO:99; SEQ ID NO:101; SEQ ID NO:103; SEQ ID
NO:105; SEQ ID NO:107; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:113; SEQ ID
NO:115; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:121; SEQ ID NO:123; SEQ ID
NO:125; SEQ ID NO:127; SEQ ID NO:129; SEQ ID NO:131; SEQ ID NO:133; SEQ ID
NO:135; SEQ ID NO:137; SEQ ID NO:139; SEQ ID NO:141; SEQ ID NO:143; SEQ ID
NO:145; SEQ ID NO:147; SEQ ID NO:149; SEQ ID NO:151; SEQ ID NO:153; SEQ ID
NO:155; SEQ ID NO:157; SEQ ID NO:159; SEQ ID NO:161; SEQ ID NO:163; SEQ ID
NO:165; SEQ ID NO:167; SEQ ID NO:169; SEQ ID NO:171; SEQ ID NO:173; SEQ ID

NO:175; SEQ ID NO:177; SEQ ID NO:179; SEQ ID NO:181; SEQ ID NO:183; SEQ ID
NO:185; SEQ ID NO:187 or SEQ ID NO:191.
100. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises hydrolysis of, or decontamination of, a P-S bond.
101. The isolated, synthetic or recombinant polypeptide or peptide of claim 100, wherein the polypeptide having P-S bond hydrolyzing or decontamination activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID NO: 75; SEQ
ID NO:
77; SEQ ID NO: 89; SEQ ID NO:117; SEQ ID NO:119; SEQ ID NO:127; SEQ ID NO:151;

SEQ ID NO:167; SEQ ID NO:171; SEQ ID NO:187 or SEQ ID NO:191.
102. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises an organophosphoric acid anhydrolase (OPAA) activity.
103. The isolated, synthetic or recombinant polypeptide or peptide of claim 102, wherein the enzyme having organophosphoric acid anhydrolase (OPAA) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:193, or having an amino acid sequence as set forth in SEQ ID NO:194.
104. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises an aminopeptidase activity.
105. The isolated, synthetic or recombinant polypeptide or peptide of claim 104, wherein the enzyme having aminopeptidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO: 189, or having an amino acid sequence as set forth in SEQ ID NO:190.
106. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a carboxylesterase activity.
107. The isolated, synthetic or recombinant polypeptide or peptide of claim 106, wherein the enzyme having carboxylesterase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:73; SEQ ID NO:75; SEQ ID NO:77; SEQ ID
NO:79;
SEQ ID NO:81; SEQ ID NO:83; SEQ ID NO:85; SEQ ID NO:87 or SEQ ID NO:89.
108. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a heme-based peroxidase activity.
109. The isolated, synthetic or recombinant polypeptide or peptide of claim 108, wherein the enzyme having heme-based peroxidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:3; SEQ ID NO:5; SEQ ID NO:7; SEQ ID NO:9;
SEQ
ID NO:11; SEQ ID NO:13; SEQ ID NO:15; SEQ ID NO:17; SEQ ID NO:19; SEQ ID
NO:21; SEQ ID NO:23; SEQ ID NO:25; SEQ ID NO:27; SEQ ID NO:29; SEQ ID NO:31;
SEQ ID NO:33; SEQ ID NO:35; SEQ ID NO:37; SEQ ID NO:39; SEQ ID NO:41; SEQ ID
NO:43; SEQ ID NO:45; SEQ ID NO:47; SEQ ID NO:49 or SEQ ID NO:51, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:4; SEQ ID NO:6; SEQ ID
NO:8; SEQ
ID NO:10; SEQ ID NO:12; SEQ ID NO:14; SEQ ID NO:16; SEQ ID NO:18; SEQ ID
NO:20; SEQ ID NO:22; SEQ ID NO:24; SEQ ID NO:26; SEQ ID NO:28; SEQ ID NO:30;
SEQ ID NO:32; SEQ ID NO:34; SEQ ID NO:36; SEQ ID NO:38; SEQ ID NO:40; SEQ ID
NO:42; SEQ ID NO:44; SEQ ID NO:46; SEQ ID NO:48; SEQ ID NO:50 or SEQ ID NO:52, respectively.
110. The isolated, synthetic or recombinant polypeptide or peptide of claim 109, wherein the enzyme has a heme-based chloroperoxidase activity and is encoded by a nucleic having a sequence as set forth in SEQ ID NO: 1, or has an amino acid sequence as set forth in SEQ ID NO:2.
111. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a non-heme-based peroxidase activity.
112. The isolated, synthetic or recombinant polypeptide or peptide of claim 111, wherein the enzyme having non-heme-based chloroperoxidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID NO:53; SEQ ID
NO:55;
SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID

NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:2;
SEQ ID
NO:54; SEQ ID NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID NO:62; SEQ ID NO:64;
SEQ ID NO:66 or SEQ ID NO:68, respectively.
113. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a dehalogenase activity.
114. The isolated, synthetic or recombinant polypeptide or peptide of claim 113, wherein the enzyme having dehalogenase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:69 or SEQ ID NO:91, or has an amino acid sequence as set forth in SEQ ID NO:70 or SEQ ID NO:92, respectively.
115. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a diisopropylfluorophosphatase (DFPase) activity.
116. The isolated, synthetic or recombinant polypeptide or peptide of claim 115, wherein the enzyme having diisopropylfluorophosphatase (DFPase) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:71, or has an amino acid sequence as set forth in SEQ ID NO:72.
117. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises an organophosphoesterase and can hydrolyze a P-S
or a P-F bond and detoxify an acetylcholinesterase- or butyrylcholinesterase-inhibitor.
118. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises a halogenation reaction to form a hypohalite from hydrogen peroxide and chloride, bromide or iodide.
119. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises catalysis of the transfer of oxygen from hydrogen peroxide to an organic substrate.
120. The isolated, synthetic or recombinant polypeptide or peptide of claim 119, wherein the enzymatic activity comprises chemical bleaching of lignin.
121. The isolated, synthetic or recombinant polypeptide or peptide of claim 120, wherein the enzymatic activity comprises chemical bleaching of lignin in a pulp or paper manufacturing process.
122. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the enzymatic activity comprises detoxifying a pesticide, herbicide and/or insecticide.
123. The isolated, synthetic or recombinant polypeptide or peptide of claim 122, wherein the pesticide is Demeton-S, Demeton-S-methyl, Demeton-S-methylsulphon, Demeton-methyl, Parathion, Phosmet, Carbophenothion, Benoxafos, Azinphos-methyl, Azinphos-ethyl, Amiton, Amidithion, Cyanthoate, Dialiphos, Dimethoate, Dioxathion, Disulfoton, Endothion, Etion, Ethoate-methyl, Formothion, Malathion, Mercarbam, Omethoate, Oxydeprofos, Oxydisulfoton, Phenkapton, Phorate, Phosalone, Prothidathion, Prothoate, Sophamide, Thiometon, Vamidothion, Methamidophos or a combination thereof.
124. The isolated, synthetic or recombinant polypeptide or peptide of any one of claims 76 to 123, wherein the nucleic acid encodes a thermostable or thermotolerant enzyme or protein.
125. The isolated, synthetic or recombinant polypeptide or peptide of claim 124, wherein the polypeptide retains enzyme activity under conditions comprising a temperature range of between about 37°C to about 95°C, or between about 55°C to about 85°C, or between about 70°C to about 75°C, or between about 70°C
to about 95°C, or between about 90°C to about 95°C, or, retains enzyme activity under conditions comprising a temperature range of from greater than 37°C to about 95°C, from greater than 55°C to about 85°C, or between about 70°C to about 75°C, or from greater than 90°C to about 95°C, or in the range between about 1°C to about 5°C, between about 5°C to about 15°C, between about 15°C to about 25°C, between about 25°C to about 37°C, between about 37°C to about 95°C.
126. The isolated, synthetic or recombinant polypeptide or peptide of claim 124, wherein the polypeptide retains an activity after exposure to a temperature in the range from greater than 37°C to about 95°C, from greater than 55°C
to about 85°C, from greater than 90°C to about 95°C, or after exposure to a temperature in the range between about 1°C to about 5°C, between about 5°C to about 15°C, between about 15°C to about 25°C, between about 25°C to about 37°C, between about 37°C to about 95°C, between about 55°C to about 85°C, between about 70°C to about 75°C, or between about 90°C to about 95°C.
127. An isolated, synthetic or recombinant polypeptide or peptide comprising a sequence as set forth in claim 76 and lacking a signal sequence.
128. An isolated, synthetic or recombinant polypeptide or peptide comprising a sequence as set forth in claim 76 and having a heterologous signal sequence.
129. The isolated, synthetic or recombinant polypeptide or peptide of claim 76 having a specific activity at about 37°C in the range from about 100 to about 1000 units per milligram of protein, from about 500 to about 750 units per milligram of protein, from about 500 to about 1200 units per milligram of protein, or from about 750 to about 1000 units per milligram of protein.
130. The isolated, synthetic or recombinant polypeptide or peptide of claim 124, wherein the thermotolerance comprises retention of at least half of the specific activity of the hydrolase at 37°C after being heated to an elevated temperature.
131. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the polypeptide comprises at least one glycosylation site, and optionally the glycosylation is an N-linked glycosylation, and optionally the polypeptide is glycosylated after being expressed in a P. pastoris or a S. pombe.
132. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the polypeptide or peptide retains enzyme or binding activity under conditions comprising about pH 6.5, pH 6.0, pH 5.5, 5.0, pH 4.5 or 4Ø
133. The isolated, synthetic or recombinant polypeptide or peptide of claim 76, wherein the polypeptide or peptide retains enzyme or binding activity under conditions comprising about pH 8.0, pH 8.5, pH 9, pH 9.5, pH 10 or pH 10.5.
134. A protein preparation comprising a polypeptide or peptide of claim 76, wherein the protein preparation comprises a liquid, a solid or a gel.
135. A heterodimer comprising a polypeptide or peptide of claim 76 and a second domain, and optionally the second domain is an epitope or a tag.
136. The heterodimer of claim 135, wherein the second domain is a polypeptide and the heterodimer is a fusion protein.
137. A homodimer comprising a polypeptide or peptide of claim 76.
138. An immobilized polypeptide or peptide, wherein the polypeptide or peptide comprises a sequence as set forth in claim 76, or a subsequence thereof.
139. The immobilized polypeptide or peptide of claim 138, wherein the polypeptide or peptide is immobilized on a cell, a metal, a resin, a polymer, a ceramic, a glass, a microelectrode, a graphitic particle, a bead, a gel, a plate, an array or a capillary tube.
140. An array comprising an immobilized polypeptide or peptide as set forth in claim 138 or claim 139.
141. An array comprising an immobilized nucleic acid as set forth in claim 1 or claim 57.
142. An isolated, synthetic or recombinant antibody that specifically binds to a polypeptide or peptide as set forth in claim 76, and optionally the antibody is a monoclonal or a polyclonal antibody.
143. A hybridoma comprising an antibody that specifically binds to a polypeptide as set forth in claim 76.
144. A method of isolating or identifying a polypeptide with an enzymatic activity or encoding a protein comprising the steps of:
(a) providing an antibody as set forth in claim 142;
(b) providing a sample comprising polypeptides; and (c) contacting the sample of step (b) with the antibody of step (a) under conditions wherein the antibody can specifically bind to the polypeptide, thereby isolating or identifying a polypeptide having enzymatic activity or encoding a protein.
145. A method of making an anti-enzyme or anti-protein antibody comprising administering to a non-human animal a nucleic acid as set forth in claim 1 or claim 57 or a subsequence thereof in an amount sufficient to generate a humoral immune response, thereby making an anti-enzyme or anti-protein antibody.
146. A method of making an anti-enzyme or anti-protein antibody comprising administering to a non-human animal a polypeptide or peptide as set forth in claim 76, or a subsequence thereof, in an amount sufficient to generate a humoral immune response, thereby making an anti-enzyme or anti-protein antibody.
147. A method of producing a recombinant polypeptide comprising the steps of:
(a) providing a nucleic acid operably linked to a promoter, wherein the nucleic acid comprises a sequence as set forth in claim 1 or claim 57; and (b) expressing the nucleic acid of step (a) under conditions that allow expression of the polypeptide, thereby producing a recombinant polypeptide.
148. The method of claim 147, further comprising transforming a host cell with the nucleic acid of step (a) followed by expressing the nucleic acid of step (a), thereby producing a recombinant polypeptide.
149. A method for identifying a polypeptide having an enzyme activity or encoding a protein comprising the following steps:

(a) providing a polypeptide as set forth in claim 76;
(b) providing an enzyme substrate; and (c) contacting the polypeptide with the substrate of step (b) and detecting a decrease in the amount of substrate or an increase in the amount of a reaction product, wherein a decrease in the amount of the substrate or an increase in the amount of the reaction product detects a polypeptide having an enzyme activity or encoding a protein.
150. A method for identifying an enzyme or protein substrate comprising the following steps:

(a) providing a polypeptide as set forth in claim 76;
(b) providing a test substrate; and (c) contacting the polypeptide of step (a) with the test substrate of step (b) and detecting a decrease in the amount of substrate or an increase in the amount of reaction product, wherein a decrease in the amount of the substrate or an increase in the amount of a reaction product identifies the test substrate as an enzyme or protein substrate.
151. A method of determining whether a test compound specifically binds to a polypeptide comprising the following steps:

(a) expressing a nucleic acid or a vector comprising the nucleic acid under conditions permissive for translation of the nucleic acid to a polypeptide, wherein the nucleic acid has a sequence as set forth in claim 1 or claim 57;
(b) providing a test compound;

(c) contacting the polypeptide with the test compound; and (d) determining whether the test compound of step (b) specifically binds to the polypeptide.
152. A method of determining whether a test compound specifically binds to a polypeptide comprising the following steps:
(a) providing a polypeptide as set forth in claim 76;
(b) providing a test compound;
(c) contacting the polypeptide with the test compound; and (d) determining whether the test compound of step (b) specifically binds to the polypeptide.
153. A method for identifying a modulator of an enzyme activity comprising the following steps:
(a) providing a polypeptide as set forth in claim 76;
(b) providing a test compound;
(c) contacting the polypeptide of step (a) with the test compound of step (b) and measuring an activity of the enzyme, wherein a change in the enzyme activity measured in the presence of the test compound compared to the activity in the absence of the test compound provides a determination that the test compound modulates the enzyme activity.
154. The method of claim 153, wherein the enzyme activity is measured by providing an enzyme substrate and detecting a decrease in the amount of the substrate or an increase in the amount of a reaction product, or, an increase in the amount of the substrate or a decrease in the amount of a reaction product, and optionally a decrease in the amount of the substrate or an increase in the amount of the reaction product with the test compound as compared to the amount of substrate or reaction product without the test compound identifies the test compound as an activator of an enzyme activity, and optionally an increase in the amount of the substrate or a decrease in the amount of the reaction product with the test compound as compared to the amount of substrate or reaction product without the test compound identifies the test compound as an inhibitor of an enzyme activity.
155. A computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a polypeptide sequence or a nucleic acid sequence, wherein the polypeptide sequence comprises sequence as set forth in claim 76, a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57.
156. The computer system of claim 155, further comprising a sequence comparison algorithm and a data storage device having at least one reference sequence stored thereon, and optionally the sequence comparison algorithm comprises a computer program that indicates polymorphisms, and optionally the computer system further comprises an identifier that identifies one or more features in said sequence.
157. A computer readable medium having stored thereon a polypeptide sequence or a nucleic acid sequence, wherein the polypeptide sequence comprises a polypeptide as set forth in claim 76, a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57.
158. A method for identifying a feature in a sequence comprising the steps of:
(a) reading the sequence using a computer program which identifies one or more features in a sequence, wherein the sequence comprises a polypeptide sequence or a nucleic acid sequence, wherein the polypeptide sequence comprises a polypeptide as set forth in claim 76, a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57;
and (b) identifying one or more features in the sequence with the computer program.
159. A method for comparing a first sequence to a second sequence comprising the steps of: (a) reading the first sequence and the second sequence through use of a computer program which compares sequences, wherein the first sequence comprises a polypeptide sequence or a nucleic acid sequence, wherein the polypeptide sequence comprises a polypeptide as set forth in claim 76, a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57; and (b) determining differences between the first sequence and the second sequence with the computer program, and optionally the step of determining differences between the first sequence and the second sequence further comprises the step of identifying polymorphisms, and optionally the method further comprises use of an identifier that identifies one or more features in a sequence and optionally the method further comprises reading the first sequence using a computer program and identifying one or more features in the sequence.
160. A method for isolating or recovering a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein from an environmental sample comprising the steps of:
(a) providing an amplification primer pair as set forth in any of claims 54 to 56;
(b) isolating a nucleic acid from the environmental sample or treating the environmental sample such that nucleic acid in the sample is accessible for hybridization to the amplification primer pair; and, (c) combining the nucleic acid of step (b) with the amplification primer pair of step (a) and amplifying nucleic acid from the environmental sample, thereby isolating or recovering a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein from an environmental sample;
and optionally each member of the amplification primer sequence pair comprises an oligonucleotide comprising at least about 10 to about 50 consecutive bases of a sequence as set forth in SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ
ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID
NO:21, SEQ ID NO:23, SEQ ID NO:25, and all nucleic acids disclosed in the SEQ
ID
listing, which include all odd numbered SEQ ID NO:s from SEQ ID NO:1 through SEQ ID
NO:193, or a subsequence thereof.
161. A method for isolating or recovering a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein from an environmental sample comprising the steps of:

(a) providing a polynucleotide probe comprising a sequence as set forth in claim 1 or claim 57, or a subsequence thereof;
(b) isolating a nucleic acid from the environmental sample or treating the environmental sample such that nucleic acid in the sample is accessible for hybridization to a polynucleotide probe of step (a);
(c) combining the isolated nucleic acid or the treated environmental sample of step (b) with the polynucleotide probe of step (a); and (d) isolating a nucleic acid that specifically hybridizes with the polynucleotide probe of step (a), thereby isolating or recovering a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein from an environmental sample.
162. The method of claim 160 or claim 161, wherein the environmental sample comprises a water sample, a liquid sample, a soil sample, an air sample or a biological sample, and optionally the biological sample is derived from a bacterial cell, a protozoan cell, an insect cell, a yeast cell, a plant cell, a fungal cell or a mammalian cell.
163. A method of generating a variant of a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein comprising the steps of:
(a) providing a template nucleic acid comprising a sequence as set forth in claim 1 or claim 57; and (b) modifying, deleting or adding one or more nucleotides in the template sequence, or a combination thereof, to generate a variant of the template nucleic acid, and optionally the method further comprises expressing the variant nucleic acid to generate a variant enzyme or protein polypeptide.
164. The method of claim 163, wherein the modifications, additions or deletions are introduced by a method comprising error-prone PCR, shuffling, oligonucleotide-.directed mutagenesis, assembly PCR, sexual PCR mutagenesis, in vivo mutagenesis, cassette mutagenesis, recursive ensemble mutagenesis, exponential ensemble mutagenesis, site-specific mutagenesis, gene reassembly, Gene Site Saturation Mutagenesis (GSSM), synthetic ligation reassembly (SLR) and a combination thereof, or, the modifications, additions or deletions are introduced by a method comprising recombination, recursive sequence recombination, phosphothioate-modified DNA mutagenesis, uracil-containing template mutagenesis, gapped duplex mutagenesis, point mismatch repair mutagenesis, repair-deficient host strain mutagenesis, chemical mutagenesis, radiogenic mutagenesis, deletion mutagenesis, restriction-selection mutagenesis, restriction-purification mutagenesis, artificial gene synthesis, ensemble mutagenesis, chimeric nucleic acid multimer creation and a combination thereof.
165. The method of claim 163, wherein the method is iteratively repeated until an enzyme or protein having an altered or different activity or an altered or different stability from that of a polypeptide encoded by the template nucleic acid is produced, and optionally the variant enzyme or protein polypeptide is thermotolerant, and retains some activity after being exposed to an elevated temperature, or the variant enzyme or protein polypeptide has increased glycosylation as compared to the enzyme or protein encoded by a template nucleic acid, or the variant enzyme or protein polypeptide has an enzyme activity under a high temperature, wherein the enzyme encoded by the template nucleic acid is not active under the high temperature and optionally the method is iteratively repeated until an enzyme or protein coding sequence having an altered codon usage from that of the template nucleic acid is produced, or the method is iteratively repeated until an enzyme or protein gene having higher or lower level of message expression or stability from that of the template nucleic acid is produced.
166. A method for modifying codons in a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein to increase its expression in a host cell, the method comprising the following steps:

(a) providing a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein comprising a sequence as set forth in claim 1 or claim 57;
and, (b) identifying a non-preferred or a less preferred codon in the nucleic acid of step (a) and replacing it with a preferred or neutrally used codon encoding the same amino acid as the replaced codon, wherein a preferred codon is a codon over-represented in coding sequences in genes in the host cell and a non-preferred or less preferred codon is a codon under-represented in coding sequences in genes in the host cell, thereby modifying the nucleic acid to increase its expression in a host cell.
167. A method for modifying codons in a nucleic acid encoding a polypeptide having an enzyme activity or encoding a protein, the method comprising the following steps:
(a) providing a nucleic acid encoding a polypeptide with an enzyme activity or encoding a protein comprising a sequence as set forth in claim 1 or claim 57;
and, (b) identifying a codon in the nucleic acid of step (a) and replacing it with a different codon encoding the same amino acid as the replaced codon, thereby modifying codons in a nucleic acid encoding an enzyme or a protein.
168. A method for modifying codons in a nucleic acid encoding a polypeptide having an enzyme activity or encoding a protein, to increase its expression in a host cell, the method comprising the following steps:
(a) providing a nucleic acid encoding a polypeptide having an enzyme activity or encoding a protein, comprising a sequence as set forth in claim 1 or claim 57;
and, (b) identifying a non-preferred or a less preferred codon in the nucleic acid of step (a) and replacing it with a preferred or neutrally used codon encoding the same amino acid as the replaced codon, wherein a preferred codon is a codon over-represented in coding sequences in genes in the host cell and a non-preferred or less preferred codon is a codon under-represented in coding sequences in genes in the host cell, thereby modifying the nucleic acid to increase its expression in a host cell.
169. A method for modifying a codon in a nucleic acid encoding a polypeptide having an enzyme activity or encoding a protein to decrease its expression in a host cell, the method comprising the following steps:
(a) providing a nucleic acid encoding a polypeptide having an enzyme activity or encoding a protein comprising a sequence as set forth in claim 1 or claim 57;
and (b) identifying at least one preferred codon in the nucleic acid of step (a) and replacing it with a non-preferred or less preferred codon encoding the same amino acid as the replaced codon, wherein a preferred codon is a codon over-represented in coding sequences in genes in a host cell and a non-preferred or less preferred codon is a codon under-represented in coding sequences in genes in the host cell, thereby modifying the nucleic acid to decrease its expression in a host cell, and optionally the host cell is a bacterial cell, a fungal cell, an insect cell, a yeast cell, a plant cell or a mammalian cell.
170. A method for producing a library of nucleic acids encoding a plurality of modified enzyme or protein active sites or substrate binding sites, wherein the modified active sites or substrate binding sites are derived from a first nucleic acid comprising a sequence encoding a first active site or a first substrate binding site the method comprising the following steps:
(a) providing a first nucleic acid encoding a first active site or first substrate binding site, wherein the first nucleic acid sequence comprises a sequence that hybridizes under stringent conditions to a sequence as set forth in SEQ ID NO:1, SEQ ID NO:3, SEQ ID
NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ
ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, and all nucleic acids disclosed in the SEQ ID listing, which include all odd numbered SEQ ID
NO:s from SEQ ID NO:1 through SEQ ID NO:193, or a subsequence thereof, and the nucleic acid encodes an enzyme or protein active site or an enzyme or protein substrate binding site;
(b) providing a set of mutagenic oligonucleotides that encode naturally-occurring amino acid variants at a plurality of targeted codons in the first nucleic acid; and, (c) using the set of mutagenic oligonucleotides to generate a set of active site-encoding or substrate binding site-encoding variant nucleic acids encoding a range of amino acid variations at each amino acid codon that was mutagenized, thereby producing a library of nucleic acids encoding a plurality of modified enzyme or protein active sites or substrate binding sites.
171. The method of claim 170, comprising mutagenizing the first nucleic acid of step (a) or variants by a method comprising an optimized directed evolution system, Gene Site Saturation Mutagenesis (GSSM), or a synthetic ligation reassembly (SLR), error-prone PCR, shuffling, oligonucleotide-directed mutagenesis, assembly PCR, sexual PCR

mutagenesis, in vivo mutagenesis, cassette mutagenesis, recursive ensemble mutagenesis, exponential ensemble mutagenesis, site-specific mutagenesis, gene reassembly, and a combination thereof; or, mutagenizing the first nucleic acid of step (a) or variants by a method comprising recombination, recursive sequence recombination, phosphothioate-modified DNA mutagenesis, uracil-containing template mutagenesis, gapped duplex mutagenesis, point mismatch repair mutagenesis, repair-deficient host strain mutagenesis, chemical mutagenesis, radiogenic mutagenesis, deletion mutagenesis, restriction-selection mutagenesis, restriction-purification mutagenesis, artificial gene synthesis, ensemble mutagenesis, chimeric nucleic acid multimer creation and a combination thereof.
172. A method for making a small molecule comprising the following steps:
(a) providing a plurality of biosynthetic enzymes capable of synthesizing or modifying a small molecule, wherein one of the enzymes comprises an enzyme encoded by a nucleic acid comprising a sequence as set forth in claim 1 or claim 57;
(b) providing a substrate for at least one of the enzymes of step (a); and (c) reacting the substrate of step (b) with the enzymes under conditions that facilitate a plurality of biocatalytic reactions to generate a small molecule by a series of biocatalytic reactions.
173. A method for modifying a small molecule comprising the following steps:
(a) providing an enzyme, wherein the enzyme comprises a polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid comprising a nucleic acid sequence as set forth in claim 1 or claim 57;
(b) providing a small molecule; and (c) reacting the enzyme of step (a) with the small molecule of step (b) under conditions that facilitate an enzymatic reaction catalyzed by the enzyme, thereby modifying a small molecule by an enzymatic reaction, and optionally the method further comprises use of a plurality of small molecule substrates for the enzyme of step (a), thereby generating a library of modified small molecules produced by at least one enzymatic reaction catalyzed by the enzyme, and optionally the method further comprises use of a plurality of additional enzymes under conditions that facilitate a plurality of biocatalytic reactions by the enzymes to form a library of modified small molecules produced by the plurality of enzymatic reactions, and optionally the method further comprises the step of testing the library to determine if a particular modified small molecule which exhibits a desired activity is present within the library, and optionally the step of testing the library further comprises the steps of systematically eliminating all but one of the biocatalytic reactions used to produce a portion of the plurality of the modified small molecules within the library by testing the portion of the modified small molecule for the presence or absence of the particular modified small molecule with a desired activity, and identifying at least one specific biocatalytic reaction that produces the particular modified small molecule of desired activity.
174. A method for determining a functional fragment of an enzyme comprising the steps of (a) providing an enzyme, wherein the enzyme comprises a polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid comprising a nucleic acid sequence as set forth in claim 1 or claim 57; and (b) deleting a plurality of amino acid residues from the sequence of step (a) and testing the remaining subsequence for an enzyme activity, thereby determining a functional fragment of an enzyme, and optionally the enzyme activity is measured by providing an enzyme substrate and detecting a decrease in the amount of the substrate or an increase in the amount of a reaction product.
175. A method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis, the method comprising the following steps:

(a) making a modified cell by modifying the genetic composition of a cell, wherein the genetic composition is modified by addition to the cell of a nucleic acid comprising a sequence as set forth in claim 1 or claim 57;
(b) culturing the modified cell to generate a plurality of modified cells;
(c) measuring at least one metabolic parameter of the cell by monitoring the cell culture of step (b) in real time; and, (d) analyzing the data of step (c) to determine if the measured parameter differs from a comparable measurement in an unmodified cell under similar conditions, thereby identifying an engineered phenotype in the cell using real-time metabolic flux analysis, and optionally the genetic composition of the cell is modified by a method comprising deletion of a sequence or modification of a sequence in the cell, or, knocking out the expression of a gene, and optionally the method further comprises selecting a cell comprising a newly engineered phenotype, and optionally the method further comprises culturing the selected cell, thereby generating a new cell strain comprising a newly engineered phenotype.
176. An isolated or recombinant signal sequence (signal peptide) consisting of, or consisting essentially of, a sequence as set forth in residues 1 to 14, 1 to 15, 1 to 16, 1 to 17, 1 to 18, 1 to 19, 1 to 20, 1 to 21, 1 to 22, 1 to 23, 1 to 24, 1 to 25, 1 to 26, 1 to 27, 1 to 28, 1 to 28, 1 to 30, 1 to 31, 1 to 32, 1 to 33, 1 to 34, 1 to 35, 1 to 36, 1 to 37, 1 to 38, 1 to 40, 1 to 41, 1 to 42, 1 to 43, 1 to 44, 1 to 45, 1 to 46, or 1 to 47, of (a) SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID
NO:22, SEQ ID NO:24, and all polypeptides disclosed in the SEQ ID listing, which include all even numbered SEQ ID NO:s from SEQ ID NO:2 through SEQ ID NO:194, or (b) a sequence as set forth in claim 1 or claim 57.
177. A chimeric polypeptide comprising at least a first domain comprising signal peptide (SP) having a sequence as set forth in claim 176, and at least a second domain comprising a heterologous polypeptide or peptide, wherein the heterologous polypeptide or peptide is not naturally associated with the signal peptide (SP), and optionally the heterologous polypeptide or peptide is not an enzyme, and optionally the heterologous polypeptide or peptide is amino terminal to, carboxy terminal to or on both ends of the signal peptide (SP) or an enzyme catalytic domain (CD).
178. A method of increasing thermotolerance or thermostability of a enzyme polypeptide, the method comprising glycosylating an enzyme, wherein the polypeptide comprises at least thirty contiguous amino acids of a polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, thereby increasing thermotolerance or thermostability of the enzyme.
179. A method for overexpressing a recombinant enzyme in a cell comprising expressing a vector comprising a nucleic acid sequence as set forth in claim 1 or claim 57, wherein overexpression is effected by use of a high activity promoter, a dicistronic vector or by gene amplification of the vector.
180. A method of making a transgenic plant comprising the following steps:
(a) introducing a heterologous nucleic acid sequence into the cell, wherein the heterologous nucleic sequence comprises a sequence as set forth in claim 1 or claim 57, thereby producing a transformed plant cell;
(b) producing a transgenic plant from the transformed cell, and optionally step (a) further comprises introducing the heterologous nucleic acid sequence by electroporation or microinjection of plant cell protoplasts, and optionally step (a) comprises introducing the heterologous nucleic acid sequence directly to plant tissue by DNA particle bombardment or by using an Agrobacterium tumefaciens host.
181. A method of expressing a heterologous nucleic acid sequence in a plant cell comprising the following steps:
(a) transforming the plant cell with a heterologous nucleic acid sequence operably linked to a promoter, wherein the heterologous nucleic sequence comprises a sequence as set forth in claim 1 or claim 57;
(b) growing the plant under conditions wherein the heterologous nucleic acids sequence is expressed in the plant cell.
182. A method for decreasing the amount of a compound in a composition comprising the following steps:
(a) providing a polypeptide having an enzyme activity or encoding a protein as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57;
(b) providing a composition comprising the compound; and (c) contacting the polypeptide of step (a) with the composition of step (b) under conditions wherein the enzyme hydrolyzes, dehalogenates, oxidizes, breaks up or otherwise processes the compound in the composition.
183. A cellulose-comprising compound comprising at least one polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, and optionally the cellulose-comprising compound comprises a pulp, a paper, a paper product, a wood, a wood product, or a paper or wood waste product.
184. A method for chemical bleaching of lignin or a cellulose in a pulp or paper manufacturing process comprising the steps of (a) providing a polypeptide having chloroperoxidase activity as set forth in claim 76, or a polypeptide having chloroperoxidase activity encoded by a nucleic acid as set forth in claim 1 or claim 57;
(b) providing a compound comprising a lignin or a cellulose; and (c) contacting the compound with the polypeptide under conditions wherein the polypeptide is enzymatically active and the lignin or a cellulose is bleached, wherein optionally the enzymatic activity comprises a heme-based peroxidase activity, and optionally the enzyme having heme-based peroxidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:3; SEQ ID NO:5; SEQ ID
NO:7; SEQ
ID NO:9; SEQ ID NO:11; SEQ ID NO:13; SEQ ID NO:15; SEQ ID NO:17; SEQ ID NO:19;

SEQ ID NO:21; SEQ ID NO:23; SEQ ID NO:25; SEQ ID NO:27; SEQ ID NO:29; SEQ ID
NO:31; SEQ ID NO:33; SEQ ID NO:35; SEQ ID NO:37; SEQ ID NO:39; SEQ ID NO:41;
SEQ ID NO:43; SEQ ID NO:45; SEQ ID NO:47; SEQ ID NO:49 or SEQ ID NO:51, or the enzyme has an amino acid sequence as set forth in SEQ ID NO:4; SEQ ID NO:6;
SEQ ID
NO:8; SEQ ID NO:10; SEQ ID NO:12; SEQ ID NO:14; SEQ ID NO:16; SEQ ID NO:18;
SEQ ID NO:20; SEQ ID NO:22; SEQ ID NO:24; SEQ ID NO:26; SEQ ID NO:28; SEQ ID
NO:30; SEQ ID NO:32; SEQ ID NO:34; SEQ ID NO:36; SEQ ID NO:38; SEQ ID NO:40;
SEQ ID NO:42; SEQ ID NO:44; SEQ ID NO:46; SEQ ID NO:48; SEQ ID NO:50 or SEQ ID

NO:52, respectively, and optionally the enzyme has a heme-based chloroperoxidase activity and is encoded by a nucleic having a sequence as set forth in SEQ ID NO: 1, or the enzyme has an amino acid sequence as set forth in SEQ ID NO:2, wherein optionally the enzymatic activity comprises a non-heme-based peroxidase activity, and optionally the enzyme having non-heme-based chloroperoxidase activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID
NO:53; SEQ
ID NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ
ID NO:2;
SEQ ID NO:54; SEQ ID NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID NO:62; SEQ ID
NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively, and optionally the cellulose or lipophilic compound comprises a pulp, a paper, a paper product, a wood, a wood product, or a paper or wood waste product.
185. A composition comprising at least one polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally the composition is formulated as an edible delivery agent, an injectable liquid, a tablet, a gel, a liposome, a pill, a capsule, a geltab, a lotion, a topical applied liquid, a suppository, a powder, a lyophilized compound, a foam, an emulsion or a combination thereof, and optionally the composition comprises at least two or three polypeptides as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally an enzyme in the composition is present at a concentration of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%, or is present at a w/v of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%.
186. A pharmaceutical composition comprising a polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally the pharmaceutical composition is formulated as an edible delivery agent, an injectable liquid, a spray, a tablet, a gel, a liposome, a capsule, a geltab, a hydrogel, a lotion, a topical applied liquid, a suppository, an aerosol, a powder, a lyophilized compound, a propellant, a foam, an emulsion, a nanostructure, an implant or a combination thereof wherein optionally an enzyme in the composition is present at a concentration of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%, or is present at a w/v of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%%.
187. A nanostructure comprising at least one polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally the nanostructure comprises a nanotubule or a nanofiber, and optionally the composition comprises at least two or three polypeptides as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57.
188. A lyophilized polypeptide having a sequence as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57.
189. A decontaminating, neutralizing or detoxifying composition comprising at least one polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally the decontaminating or detoxifying composition is formulated as an edible delivery agent, an injectable liquid, a tablet, a gel, a liposome, a capsule, a geltab, a lotion, a topical applied liquid, a suppository, a powder, a lyophilized compound, a foam, an emulsion or a combination thereof, wherein optionally the composition comprises at least two or three polypeptides as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally an enzyme in the composition is present at a concentration of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%, or is present at a w/v of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%.
190. The decontaminating, neutralizing or detoxifying composition of claim 189, further comprising a surfactant, an emulsifier, a foaming agent or a combination thereof.
191. The decontaminating, neutralizing or detoxifying composition of claim 189, wherein the composition is formulated as a pesticide, herbicide and/or insecticide detoxifying agent.
192. The decontaminating, neutralizing or detoxifying composition of claim 189, wherein the composition is formulated as a nerve gas detoxifying agent.
193. The decontaminating, neutralizing or detoxifying composition of claim 189, wherein when the composition comprises a haloperoxidase enzyme, the composition also comprises halite component, and optionally the halite component comprises a chlorite component, and optionally the chlorite component comprises a sodium chlorite or a sodium iodite.
194. A product of manufacture comprising at least one polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57.
195. The product of manufacture of claim 194, wherein when the product of manufacture comprises a haloperoxidase enzyme, the composition also comprises halite component, and optionally the halite component comprises a chlorite component, and optionally the chlorite component comprises a sodium chlorite or a sodium iodite.
196. A cloth, textile or fiber comprising at least one polypeptide as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57, wherein optionally the at least one polypeptide is immobilized onto the surface of the cloth, textile or fiber, or the at least one polypeptide is a component of a coating or covering on the surface of the cloth, textile or fiber, or a formulation embedded or washed into or onto the cloth, textile or fiber, wherein optionally the composition comprises at least two or three polypeptides as set forth in claim 76, or a polypeptide encoded by a nucleic acid as set forth in claim 1 or claim 57,
197. The decontaminating, neutralizing or detoxifying composition of claim 189, comprising a mixture of at least two, three, four, five or six different classes of enzymes, wherein optionally each class of enzyme detoxifies a different toxic agent or biological agent.
198. The decontaminating, neutralizing or detoxifying composition of claim 197, comprising a mixture of at least three different classes of enzymes comprising at least one dehalogenase, at least one haloperoxidase, and at least one organophosphoric acid anhydrolase (OPAA), and optionally the haloperoxidase is a chloroperoxidase.
199. The decontaminating, neutralizing or detoxifying composition of claim 198, wherein (a) the dehalogenase is a polypeptide having a sequence as set forth in SEQ ID
NO:69 or SEQ ID NO:91, or has an amino acid sequence as set forth in SEQ ID
NO:70 or SEQ ID NO:92, respectively, or (b) the chloroperoxidase is a heme-based chloroperoxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO: 1, or having an amino acid sequence as set forth in SEQ ID NO:2; or (c) the haloperoxidase is a heme-based peroxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO:3;
SEQ ID
NO:5; SEQ ID NO:7; SEQ ID NO:9; SEQ ID NO:11; SEQ ID NO:13; SEQ ID NO:15; SEQ
ID NO:17; SEQ ID NO:19; SEQ ID NO:21; SEQ ID NO:23; SEQ ID NO:25; SEQ ID
NO:27; SEQ ID NO:29; SEQ ID NO:31; SEQ ID NO:33; SEQ ID NO:35; SEQ ID NO:37;
SEQ ID NO:39; SEQ ID NO:41; SEQ ID NO:43; SEQ ID NO:45; SEQ ID NO:47; SEQ ID
NO:49 or SEQ ID NO:51, or, the enzyme has an amino acid sequence as set forth in SEQ ID
NO:4; SEQ ID NO:6; SEQ ID NO:8; SEQ ID NO:10; SEQ ID NO:12; SEQ ID NO:14; SEQ
ID NO:16; SEQ ID NO:18; SEQ ID NO:20; SEQ ID NO:22; SEQ ID NO:24; SEQ ID
NO:26; SEQ ID NO:28; SEQ ID NO:30; SEQ ID NO:32; SEQ ID NO:34; SEQ ID NO:36;
SEQ ID NO:38; SEQ ID NO:40; SEQ ID NO:42; SEQ ID NO:44; SEQ ID NO:46; SEQ ID
NO:48; SEQ ID NO:50 or SEQ ID NO:52, respectively; or (d) the haloperoxidase is a non-heme-based chloroperoxidase encoded by a nucleic having a sequence as set forth in SEQ ID
NO:1; SEQ ID NO:53; SEQ ID NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61;
SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:2; SEQ ID NO:54; SEQ ID NO:56; SEQ ID
NO:58;
SEQ ID NO:60; SEQ ID NO:62; SEQ ID NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively; or (e) the organophosphoric acid anhydrolase (OPAA) is encoded by a nucleic having a sequence as set forth in SEQ ID NO: 193, or having a sequence as set forth in SEQ
ID NO:194.
200. The decontaminating, neutralizing or detoxifying composition of claim 197, comprising a mixture of at least two different classes of enzymes comprising at least one diisopropylfluorophosphatase (DFPase) or organophosphoric acid anhydrolase (OPAA), and at least one haloperoxidase, and optionally the haloperoxidase is a chloroperoxidase.
201. The decontaminating, neutralizing or detoxifying composition of claim 200, comprising a diisopropylfluorophosphatase (DFPase), a organophosphoric acid anhydrolase (OPAA) and a haloperoxidase.
202. The decontaminating, neutralizing or detoxifying composition of claim 200, wherein the DFPase and/or OPAA are used to decontaminate, neutralize or detoxify G
agents, and the haloperoxidase or chloroperoxidase (CPO) are used to decontaminate, neutralize or detoxify V agents, H agents and/or biological agents, wherein optionally the biological agent is a bacterial spore.
203. The decontaminating, neutralizing or detoxifying composition of claim 200, further comprising a dehalogenase.
204. The decontaminating, neutralizing or detoxifying composition of claim 200, further comprising a cholinesterase.
205. The decontaminating, neutralizing or detoxifying composition of claim 200, wherein the diisopropyl-fluorophosphatase (DFPase) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:71, or has an amino acid sequence as set forth in SEQ
ID NO:72.
206. The decontaminating, neutralizing or detoxifying composition of claim 200, wherein the haloperoxidase is a chloroperoxidase.
207. The decontaminating, neutralizing or detoxifying composition of claim 206, wherein the chloroperoxidase is a heme-based chloroperoxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO: 1, or having an amino acid sequence as set forth in SEQ ID NO:2; or the haloperoxidase is a heme-based peroxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO:3; SEQ ID NO:5; SEQ ID NO:7; SEQ
ID
NO:9; SEQ ID NO:11; SEQ ID NO:13; SEQ ID NO:15; SEQ ID NO:17; SEQ ID NO:19;
SEQ ID NO:21; SEQ ID NO:23; SEQ ID NO:25; SEQ ID NO:27; SEQ ID NO:29; SEQ ID
NO:31; SEQ ID NO:33; SEQ ID NO:35; SEQ ID NO:37; SEQ ID NO:39; SEQ ID NO:41;
SEQ ID NO:43; SEQ ID NO:45; SEQ ID NO:47; SEQ ID NO:49 or SEQ ID NO:51, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:4; SEQ ID NO:6;
SEQ ID
NO:8; SEQ ID NO:10; SEQ ID NO:12; SEQ ID NO:14; SEQ ID NO:16; SEQ ID NO:18;
SEQ ID NO:20; SEQ ID NO:22; SEQ ID NO:24; SEQ ID NO:26; SEQ ID NO:28; SEQ ID
NO:30; SEQ ID NO:32; SEQ ID NO:34; SEQ ID NO:36; SEQ ID NO:38; SEQ ID NO:40;
SEQ ID NO:42; SEQ ID NO:44; SEQ ID NO:46; SEQ ID NO:48; SEQ ID NO:50 or SEQ ID

NO:52, respectively; or the haloperoxidase is a non-heme-based chloroperoxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID NO:53; SEQ
ID
NO:55; SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID
NO:2;
SEQ ID NO:54; SEQ ID NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID NO:62; SEQ ID
NO:64; SEQ ID NO:66 or SEQ ID NO:68, respectively.
208. The decontaminating, neutralizing or detoxifying composition of claim 200, wherein the organophosphoric acid anhydrolase (OPAA) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:193, or having an amino acid sequence as set forth in SEQ ID NO:194.
209. The decontaminating, neutralizing or detoxifying composition of claim 197, comprising a mixture of at least two different classes of enzymes comprising at least one dehalogenase (DH) and at least one haloperoxidase, wherein optionally the haloperoxidase is a chloroperoxidase (CPO).
210. The decontaminating, neutralizing or detoxifying composition of claim 209, wherein the dehalogenases and haloperoxidases are used to decontaminate, neutralize or detoxify H agents.
211. The decontaminating, neutralizing or detoxifying composition of claim 209, wherein the haloperoxidase is a heme-based chloroperoxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO: 1, or having an amino acid sequence as set forth in SEQ
ID NO:2; or the haloperoxidase is a heme-based peroxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO:3; SEQ ID NO:5; SEQ ID NO:7; SEQ ID NO:9;
SEQ
ID NO:11; SEQ ID NO:13; SEQ ID NO:15; SEQ ID NO:17; SEQ ID NO:19; SEQ ID
NO:21; SEQ ID NO:23; SEQ ID NO:25; SEQ ID NO:27; SEQ ID NO:29; SEQ ID NO:31;
SEQ ID NO:33; SEQ ID NO:35; SEQ ID NO:37; SEQ ID NO:39; SEQ ID NO:41; SEQ ID
NO:43; SEQ ID NO:45; SEQ ID NO:47; SEQ ID NO:49 or SEQ ID NO:51, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:4; SEQ ID NO:6; SEQ ID
NO:8; SEQ
ID NO:10; SEQ ID NO:12; SEQ ID NO:14; SEQ ID NO:16; SEQ ID NO:18; SEQ ID
NO:20; SEQ ID NO:22; SEQ ID NO:24; SEQ ID NO:26; SEQ ID NO:28; SEQ ID NO:30;
SEQ ID NO:32; SEQ ID NO:34; SEQ ID NO:36; SEQ ID NO:38; SEQ ID NO:40; SEQ ID
NO:42; SEQ ID NO:44; SEQ ID NO:46; SEQ ID NO:48; SEQ ID NO:50 or SEQ ID NO:52, respectively; or the haloperoxidase is a non-heme-based chloroperoxidase encoded by a nucleic having a sequence as set forth in SEQ ID NO:1; SEQ ID NO:53; SEQ ID
NO:55;
SEQ ID NO:57; SEQ ID NO:59; SEQ ID NO:61; SEQ ID NO:63; SEQ ID NO:65 or SEQ ID

NO:67, or, the enzyme has an amino acid sequence as set forth in SEQ ID NO:2;
SEQ ID
NO:54; SEQ ID NO:56; SEQ ID NO:58; SEQ ID NO:60; SEQ ID NO:62; SEQ ID NO:64;
SEQ ID NO:66 or SEQ ID NO:68, respectively.
212. The decontaminating, neutralizing or detoxifying composition of claim 209, wherein the dehalogenase has a sequence as set forth in SEQ ID NO:69 or SEQ ID
NO:91, or has an amino acid sequence as set forth in SEQ ID NO:70 or SEQ ID NO:92, respectively.
213. The decontaminating, neutralizing or detoxifying composition of claim 209, further comprising adding at least one diisopropylfluorophosphatase (DFPase), organophosphoric acid anhydrolase (OPAA) and/or cholinesterase to decontaminate, neutralize or detoxify G agents.
214. The decontaminating, neutralizing or detoxifying composition of claim 213, wherein the diisopropyl-fluorophosphatase (DFPase) is encoded by a nucleic acid having a sequence as set forth in SEQ ID NO:71, or has an amino acid sequence as set forth in SEQ
ID NO:72.
215. The decontaminating, neutralizing or detoxifying composition of claim 213, wherein the organophosphoric acid anhydrolase (OPAA) activity is encoded by a nucleic having a sequence as set forth in SEQ ID NO:193, or having an amino acid sequence as set forth in SEQ ID NO:194.
216. The decontaminating, neutralizing or detoxifying composition of claim 197, comprising a mixture of at least two different classes of enzymes comprising at least one dehalogenase (DH) enzyme and at least one organophosphoric acid anhydrolase (OPAA).
217. The decontaminating, neutralizing or detoxifying composition of claim 216, wherein the dehalogenases is used to decontaminate, neutralize or detoxify an H agent, and the organophosphoric acid anhydrolase is used to decontaminate, neutralize or detoxify G
agents.
218. The decontaminating, neutralizing or detoxifying composition of claim 216, further comprising at least one haloperoxidase to augment the decontamination, neutralization, detoxification of H agents.
219. The decontaminating, neutralizing or detoxifying composition of claim 216, further comprising a haloperoxidase, and optionally the composition can also comprise a halite component, wherein optionally the halite component comprises an iodite or a chlorite component; and optionally the iodite or a chlorite component comprises a sodium chlorite or a sodium iodite or equivalent components.
220. The decontaminating, neutralizing or detoxifying composition of claim 216, further comprising at least one diisopropylfluorophosphatase (DFPase) and/or cholinesterase enzyme to supplement the OPAA decontamination, neutralization or detoxification of a G
agent.
221. A foaming agent comprising a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, wherein optionally an enzyme in the foaming agent is present at a concentration of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%, or is present at a w/v of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%.
222. An emulsifying agent or a surfactant comprising a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, wherein optionally an enzyme in the foaming agent or surfactant is present at.
a concentration of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%, or is present at a w/v of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%.
223. A paint or coating comprising a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, wherein optionally an enzyme in the paint or coating is present at a concentration of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%, or is present at a w/v of about 0.25%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20% or 25%.
224. A method for decontaminating, neutralizing or detoxifying a toxic agent comprising application or administration of a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186.
225. The method of claim 224, wherein the composition is formulated as an edible delivery agent, an injectable liquid, a tablet, a gel, a liposome, a capsule, a geltab, a lotion, a topical applied liquid, a suppository, a powder, a lyophilized compound, a foam, an emulsion or a combination thereof.
226. A method for preventing the toxic effects of a V agent, an H agent, a G
agent or a biological agent comprising application or administration of a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186.
227. A gas mask comprising a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186.
228. An air or water filter comprising a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186.
229. A pesticide, herbicide and/or insecticide decontaminating, neutralizing or detoxifying agent comprising a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186, wherein optionally an enzyme in the composition is present at a concentration of 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, or is present at a w/v of 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%.
230. The pesticide, herbicide and/or insecticide decontaminating, neutralizing or detoxifying agent of claim 229, wherein the composition comprises an organophosphoesterase that hydrolyzes P-S or P-F bonds.
231. The pesticide, herbicide and/or insecticide decontaminating, neutralizing or detoxifying agent of claim 230, wherein the organophosphoesterase acts as an inhibitor of an acetyl-cholinesterases or a butyrylcholinesterase.
232. The pesticide, herbicide and/or insecticide decontaminating, neutralizing or detoxifying agent of claim 229, wherein the pesticide is Demeton-S, Demeton-S-methyl, Demeton-S-methylsulphon, Demeton-methyl, Parathion, Phosmet, Carbophenothion, Benoxafos, Azinphos-methyl, Azinphos-ethyl, Amiton, Amidithion, Cyanthoate, Dialiphos, Dimethoate, Dioxathion, Disulfoton, Endothion, Etion, Ethoate-methyl, Formothion, Malathion, Mercarbam, Omethoate, Oxydeprofos, Oxydisulfoton, Phenkapton, Phorate, Phosalone, Prothidathion, Prothoate, Sophamide, Thiometon, Vamidothion, Methamidophos or a combination thereof.
233. The pesticide, herbicide and/or insecticide decontaminating, neutralizing or detoxifying agent of claim 229, wherein the composition is formulated as or with a coating, a paint, a foam, a liquid, a gel, a lotion, a surfactant, a powder or an emulsifier.
234. A method for decontaminating, neutralizing or detoxifying a pesticide, herbicide and/or insecticide comprising application or administration of a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186, or the pesticide decontaminating, neutralizing or detoxifying agent of any of claims 228 to 232.
235. A method for preventing the toxic effects of a pesticide, herbicide and/or insecticide comprising application or administration of a decontaminating, neutralizing or detoxifying composition of any one of claims 189 to 193 or any one of claims 197 to 221, or a pharmaceutical composition as set forth in claim 186, or the pesticide decontaminating, neutralizing or detoxifying agent of any of claims 228 to 232.
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