ES2622062T3 - Ensayo y sistema de captura de híbrido de resultados rápidos - Google Patents
Ensayo y sistema de captura de híbrido de resultados rápidos Download PDFInfo
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- ES2622062T3 ES2622062T3 ES09752940.8T ES09752940T ES2622062T3 ES 2622062 T3 ES2622062 T3 ES 2622062T3 ES 09752940 T ES09752940 T ES 09752940T ES 2622062 T3 ES2622062 T3 ES 2622062T3
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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| EP2697397B1 (en) | 2011-04-15 | 2017-04-05 | The Johns Hopkins University | Safe sequencing system |
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| JP6061313B2 (ja) | 2012-02-13 | 2017-01-18 | ニューモデックス モレキュラー インコーポレイテッドＮｅｕｍｏｄｘ Ｍｏｌｅｃｕｌｅｒ，Ｉｎｃ． | 核酸を処理及び検出するためのマイクロ流体カートリッジ |
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| WO2013123440A1 (en) * | 2012-02-17 | 2013-08-22 | Institute For Systems Biology | Rnase h-based rna profiling |
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| JP6678585B2 (ja) | 2013-12-20 | 2020-04-22 | バイオメッド バレー ディスカバリーズ，インコーポレイティド | Ｅｒｋ阻害剤およびｒａｆ阻害剤の組み合わせを使用するがん処置 |
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| US11007183B2 (en) | 2013-12-20 | 2021-05-18 | Biomed Valley Discoveries, Inc. | Cancer treatments using combinations of PI3K/Akt pathway and ERK inhibitors |
| WO2015095825A1 (en) | 2013-12-20 | 2015-06-25 | Biomed Valley Discoveries, Inc. | Cancer treatments using combinations of type 2 mek and erk inhibitors |
| WO2015095842A2 (en) | 2013-12-20 | 2015-06-25 | Biomed Valley Discoveries, Inc. | Methods and compositions for treating non-erk mapk pathway inhibitor-resistant cancers |
| CN104020291B (zh) * | 2014-06-26 | 2016-03-30 | 南京大学 | 一种基于酶联免疫分析的hpv核酸检测试剂盒及其应用 |
| WO2016011798A1 (zh) * | 2015-01-27 | 2016-01-28 | 深圳华大基因研究院 | 用于保存生物样品的稳定剂 |
| CN108027379B (zh) | 2015-06-26 | 2021-07-23 | 雅培实验室 | 用于诊断分析设备的反应容器交换装置 |
| WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| WO2018160907A1 (en) * | 2017-03-03 | 2018-09-07 | Counsyl, Inc. | Extraction of nucleic acids for reduced probe count variability |
| CN120400338A (zh) | 2017-08-07 | 2025-08-01 | 约翰斯霍普金斯大学 | 用于评估和治疗癌症的方法和材料 |
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| IL295297A (en) | 2020-02-14 | 2022-10-01 | Univ Johns Hopkins | Methods and materials for assessing nucleic acids |
| CA3180452A1 (en) | 2020-05-27 | 2021-12-02 | Sanford D. Markowitz | Compositions and methods for preserving dna methylation |
| US11421217B1 (en) | 2021-04-07 | 2022-08-23 | Convergent Genomics, Inc. | Methods and compositions for collection and preservation of biologicals |
Family Cites Families (196)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732847A (en) | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
| FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| US4486536A (en) | 1982-05-28 | 1984-12-04 | Smithkline Diagnostics, Inc. | Specimen slide for occult blood testing |
| US4865980A (en) | 1982-12-29 | 1989-09-12 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
| ATE98300T1 (de) | 1983-01-10 | 1993-12-15 | Gen Probe Inc | Verfahren zum aufspueren, identifizieren und quantifizieren von organismen und viren. |
| US5288611A (en) | 1983-01-10 | 1994-02-22 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4743535A (en) | 1984-11-07 | 1988-05-10 | Miles Inc. | Hybridization assay employing labeled probe and anti-hybrid |
| EP0144914A3 (en) | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridization assay employing labeled pairs of hybrid binding reagents |
| US7138505B1 (en) | 1984-01-12 | 2006-11-21 | Novartis Vaccines And Diagnostics, Inc. | Factor VIII:C nucleic acid molecules |
| AU3844485A (en) | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
| FI71768C (fi) | 1984-02-17 | 1987-02-09 | Orion Yhtymae Oy | Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning. |
| US4512442A (en) * | 1984-03-30 | 1985-04-23 | Westinghouse Electric Corp. | Method and apparatus for improving the servicing of an elevator system |
| CA1260372A (en) | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
| US6221581B1 (en) | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| ZA853756B (en) | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
| CA1253777A (en) | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Nucleic acid hybridization assay employing immobilized rna probes |
| IL75648A0 (en) | 1984-07-05 | 1985-10-31 | Gen Probe Inc | Accelerated nucleic acid reassociation method |
| US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
| US4775619A (en) | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| US4689294A (en) * | 1984-11-19 | 1987-08-25 | Miles Laboratories, Inc. | Enhancement of hybridization of nucleic acids by anionic polymers |
| ES8707343A1 (es) | 1985-06-13 | 1987-07-16 | Amgen | Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada |
| US5641630A (en) | 1985-06-13 | 1997-06-24 | Amgen Inc. | Method and kit for performing nucleic acid hybridization assays |
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US4710472A (en) * | 1985-09-25 | 1987-12-01 | The United States Of America As Represented By The Secretary Of The Navy | Magnetic separation device |
| US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US5220013A (en) * | 1986-11-17 | 1993-06-15 | Scios Nova Inc. | DNA sequence useful for the detection of Alzheimer's disease |
| EP0272009B2 (en) | 1986-11-24 | 2007-10-03 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| AU601021B2 (en) | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
| IL85551A0 (en) | 1987-04-01 | 1988-08-31 | Miles Inc | Rapid hybridization assay and reagent system used therein |
| US5656731A (en) * | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
| US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| AU2932889A (en) | 1987-12-01 | 1989-07-05 | Amoco Corporation | Detection of salmonella |
| US5082830A (en) * | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| DE68916671T2 (de) | 1988-03-18 | 1995-03-02 | Baylor College Medicine | Mutationsnachweis durch kompetitiven Oligonukleotid-Priming. |
| US6326136B1 (en) * | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
| US5374524A (en) | 1988-05-10 | 1994-12-20 | E. I. Du Pont De Nemours And Company | Solution sandwich hybridization, capture and detection of amplified nucleic acids |
| NO165894C (no) | 1988-05-24 | 1991-04-24 | Gunnar Paulsen | Analysemetode for gener. |
| DE3856224T2 (de) | 1988-09-29 | 1998-12-03 | Chiron Corp., Emeryville, Calif. | Nachweis eines Polynukleotids durch Ersätzung einer Kette an einer Fangsonde |
| GB8823869D0 (en) * | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| EP0463036B1 (en) * | 1989-03-10 | 1995-02-22 | Amoco Corporation | Immobilized oligonucleotide probes and uses therefor |
| US5106727A (en) * | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| ATE174926T1 (de) | 1989-06-30 | 1999-01-15 | Chiron Corp | Hydrophobe nukleinsäure-sonde |
| US5116734A (en) * | 1989-09-01 | 1992-05-26 | Digene Diagnostics, Inc. | Highly sensitive method for detecting peroxidase |
| AU6966991A (en) | 1989-12-01 | 1991-06-26 | Gene-Trak Systems | Detection of hpv transcripts |
| US5629153A (en) | 1990-01-10 | 1997-05-13 | Chiron Corporation | Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays |
| US5792606A (en) | 1990-02-26 | 1998-08-11 | Boehringer Mannheim Gmbh | Nucleic acid hybridization based assay for determining a substance of interest |
| CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| US5695926A (en) | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US5484699A (en) * | 1990-09-28 | 1996-01-16 | Abbott Laboratories | Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods |
| US5210704A (en) * | 1990-10-02 | 1993-05-11 | Technology International Incorporated | System for prognosis and diagnostics of failure and wearout monitoring and for prediction of life expectancy of helicopter gearboxes and other rotating equipment |
| CA2095611A1 (en) | 1990-11-14 | 1992-05-15 | Jennifer Kyoko Ishii | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| KR100242671B1 (ko) | 1991-03-07 | 2000-03-02 | 고돈 에릭 | 유전학적으로 처리한 백신 균주 |
| US5283175A (en) | 1991-04-15 | 1994-02-01 | The Research Foundation Of State University Of New York | Genus-specific oligomers of Borrelia and methods of using same |
| US5556748A (en) | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
| JP3091492B2 (ja) | 1991-11-14 | 2000-09-25 | ダイジーン ダイアグノスティクス，インコーポレイテッド | 非放射性ハイブリダイゼーションアッセイおよびキット |
| US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
| US5747244A (en) | 1991-12-23 | 1998-05-05 | Chiron Corporation | Nucleic acid probes immobilized on polystyrene surfaces |
| ATE199158T1 (de) | 1991-12-23 | 2001-02-15 | Bayer Ag | Hbv amplifizierungssonden zur verwendung in hybridisierungssandwichassay in der lösungsphase |
| US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| WO1994006859A1 (en) * | 1992-09-15 | 1994-03-31 | The Dow Chemical Company | Impact modification of thermoplastics |
| JPH08503606A (ja) | 1992-10-09 | 1996-04-23 | アモコ・コーポレーション | 検定法 |
| US5400267A (en) * | 1992-12-08 | 1995-03-21 | Hemostatix Corporation | Local in-device memory feature for electrically powered medical equipment |
| EP0688366B1 (en) * | 1993-01-15 | 2002-05-22 | The Public Health Research Institute Of The City Of New York, Inc. | Sensitive nucleic acid sandwich hybridization assays and kits |
| WO1994028156A1 (en) | 1993-05-20 | 1994-12-08 | Dana-Farber Cancer Institute | Compositions and methods for treatment of herpesvirus infections |
| AU689789B2 (en) * | 1993-07-23 | 1998-04-09 | Gen-Probe Incorporated | Methods for enhancing nucleic acid amplification |
| DE4331012A1 (de) * | 1993-09-13 | 1995-03-16 | Bayer Ag | Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| DK145493D0 (da) | 1993-12-23 | 1993-12-23 | Dako As | Antistof |
| AUPM358894A0 (en) | 1994-01-31 | 1994-02-24 | Csl Limited | Modified papilloma virus l2 protein and vlps formed therefrom |
| ATE362977T1 (de) | 1994-02-14 | 2007-06-15 | Macfarlane Burnet Ct For Medic | Nicht pathogene stämme des hiv-1 |
| BR9507343A (pt) | 1994-04-04 | 1997-09-16 | Ciba Corning Diagnostics Corp | Testes de hibridização - ligação para a detecção de sequências de ácido nucléico específicas |
| CA2126952C (en) | 1994-06-28 | 2010-12-14 | Sithian Pandian | Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| JP3093116B2 (ja) * | 1994-09-30 | 2000-10-03 | 株式会社豊田中央研究所 | 核酸検出方法 |
| US6057099A (en) | 1994-12-02 | 2000-05-02 | Intelligene Ltd. | Detection of nucleic acid sequences |
| US5747248A (en) | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US20030104361A1 (en) | 1997-09-29 | 2003-06-05 | Susan Weininger | Method of detection of nucleic acids with a specific sequence composition |
| CA2139070C (en) | 1994-12-23 | 2010-03-30 | Burton W. Blais | Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction |
| US5731153A (en) * | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
| US5888724A (en) | 1995-02-17 | 1999-03-30 | The Trustees Of Columbia University In The City Of New York | Detection of high oncogenic-risk papilloma virus in high grade cervical lesions and cancers by a PCR/ELISA assay |
| WO1996040992A2 (en) | 1995-06-07 | 1996-12-19 | Abbott Laboratories | Probe masking method of reducing background in an amplification reaction |
| US6083925A (en) | 1995-06-07 | 2000-07-04 | Connaught Laboratories Limited | Nucleic acid respiratory syncytial virus vaccines |
| FR2737502B1 (fr) | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
| WO1997018334A2 (en) | 1995-11-15 | 1997-05-22 | Gen-Probe Incorporated | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
| CA2244891C (en) | 1996-02-09 | 2008-12-30 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| EP0910299B1 (en) * | 1996-02-15 | 2003-02-12 | Biosense, Inc. | Method for configuring and operating a probe |
| US20010039007A1 (en) * | 1996-10-15 | 2001-11-08 | Robert Hallowitz | Methods and compositions for determining latent viral load |
| US5853993A (en) | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| US6117631A (en) | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| GB9624165D0 (en) | 1996-11-19 | 1997-01-08 | Amdex A S | Use of nucleic acids bound to carrier macromolecules |
| US6110676A (en) | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
| US6132368A (en) * | 1996-12-12 | 2000-10-17 | Intuitive Surgical, Inc. | Multi-component telepresence system and method |
| US6331181B1 (en) * | 1998-12-08 | 2001-12-18 | Intuitive Surgical, Inc. | Surgical robotic tools, data architecture, and use |
| US20020034737A1 (en) | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| JPH10210982A (ja) * | 1997-01-31 | 1998-08-11 | Fuji Yakuhin Kogyo Kk | 新規なタンパク質 |
| US6151039A (en) * | 1997-06-04 | 2000-11-21 | Hewlett-Packard Company | Ink level estimation using drop count and ink level sense |
| US6043038A (en) | 1998-03-31 | 2000-03-28 | Tularik, Inc. | High-throughput screening assays for modulators of primase activity |
| AU7986398A (en) * | 1997-06-25 | 1999-01-04 | Tularik Inc. | High-throughput screening assays for modulators of primase activity |
| US5843995A (en) | 1997-07-07 | 1998-12-01 | University Of Medicine And Dentistry Of New Jersey | Inhibition of HIV-1 replication using oligocarbamate derivatives |
| IL121348A0 (en) * | 1997-07-21 | 1998-04-05 | Bio Rad Lab Israel Inc | System and method for device monitoring |
| JP2002505071A (ja) | 1997-11-04 | 2002-02-19 | ロシュ ダイアグノスティックス ゲーエムベーハー | 特異的かつ高感度な核酸検出方法 |
| US6225053B1 (en) | 1997-12-12 | 2001-05-01 | Digene Corporation | Detection of hepatitis B virus |
| BR9814271A (pt) | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
| US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| EP1865074B1 (en) | 1997-12-22 | 2011-04-20 | Hitachi Chemical Co., Ltd. | Direct RT-PCR on oligonucleotide-immobilized PCR microplates |
| EP1047794A2 (en) | 1998-01-13 | 2000-11-02 | BioChip Technologies GmbH | Method for the detection or nucleic acid of nucleic acid sequences |
| AU3027699A (en) | 1998-02-02 | 1999-08-16 | Amersham Pharmacia Biotech Ab | Nucleic acid analysis method |
| US5994079A (en) | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
| US6686151B1 (en) | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
| US7399589B2 (en) | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
| ES2279565T3 (es) * | 1998-02-10 | 2007-08-16 | Biosense Webster, Inc. | Conjunto de sonda para calibracion mejorada de cateter. |
| EP0945627B1 (en) | 1998-03-23 | 2004-01-02 | SPAL S.r.l. | Axial flow fan |
| US6183956B1 (en) | 1998-03-31 | 2001-02-06 | Tularik, Incorporated | High throughput in vitro screening assays for transcription modulators |
| DE69940850D1 (de) * | 1998-08-04 | 2009-06-18 | Intuitive Surgical Inc | Gelenkvorrichtung zur Positionierung eines Manipulators für Robotik-Chirurgie |
| US20020004198A1 (en) * | 1998-12-16 | 2002-01-10 | Hardwicke Mary Ann | Novel anti-infectives |
| AU4051300A (en) | 1999-04-02 | 2000-10-23 | Tropix, Inc. | High throughput and high sensitivity detection assays |
| US20010055766A1 (en) * | 1999-04-02 | 2001-12-27 | Alexander Aristarkhov | Immunosorbant assay using branched bis-biotin/avidin/multiple label complex as a detection reagent |
| US7019822B1 (en) | 1999-04-29 | 2006-03-28 | Mss, Inc. | Multi-grade object sorting system and method |
| US6544732B1 (en) | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
| US6200746B1 (en) | 1999-08-25 | 2001-03-13 | Pharmacia & Upjohn Company | Methods of identifying anti-viral agents |
| US6611793B1 (en) * | 1999-09-07 | 2003-08-26 | Scimed Life Systems, Inc. | Systems and methods to identify and disable re-use single use devices based on detecting environmental changes |
| US20060275784A1 (en) | 1999-10-26 | 2006-12-07 | Ventana Medical Systems, Inc. | Detection of Human Papilloma Virus in Papanicolaou (Pap) Smears |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US6436662B1 (en) | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
| US6521190B1 (en) * | 2000-05-19 | 2003-02-18 | Digene Corporation | Cell collection apparatus |
| AU2001274869A1 (en) | 2000-05-20 | 2001-12-03 | The Regents Of The University Of Michigan | Method of producing a dna library using positional amplification |
| US7230092B2 (en) | 2000-06-06 | 2007-06-12 | Luminex Molecular Diagnostics, Inc. | Capture moieties for nucleic acids and uses thereof |
| US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| AU2002215488B8 (en) | 2000-06-21 | 2007-11-15 | Qiagen Gaithersburg, Inc. | Universal collection medium |
| JP2004505263A (ja) * | 2000-08-01 | 2004-02-19 | エスリング・ミルコ | 距離及び／又は位置を測定する測定装置及び測定方法 |
| EP1351970A2 (en) | 2000-12-12 | 2003-10-15 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
| EP2363476B1 (en) | 2001-02-16 | 2013-05-29 | Promega Corporation | Magnetic isolation and purification of nucleic acids |
| US6542856B2 (en) * | 2001-06-15 | 2003-04-01 | General Electric Company | System and method for monitoring gas turbine plants |
| CA2450199C (en) * | 2001-06-20 | 2009-10-13 | Power Medical Interventions, Inc. | A method and system for integrated medical tracking |
| SI1421200T1 (sl) | 2001-08-23 | 2007-04-30 | Merck & Co Inc | Poskusi PCR fluorescentnih multipleks HPV-jev z uporabo vec fluoroforov |
| US20030078228A1 (en) * | 2001-09-24 | 2003-04-24 | Taylor Doris A. | Fas Ligand |
| LU90840B1 (en) * | 2001-09-25 | 2003-03-26 | Delphi Tech Inc | Method for controlling the operation of a system sub-system or component |
| US20050032105A1 (en) | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| WO2003057914A2 (en) | 2002-01-07 | 2003-07-17 | Norchip A/S | METHOD FOR DETECTING HUMAN PAPILLOMAVIRUS mRNA |
| JP4521152B2 (ja) * | 2002-03-05 | 2010-08-11 | 株式会社東芝 | 半導体製造装置 |
| US20030175828A1 (en) | 2002-03-15 | 2003-09-18 | Lazar James G. | Signal amplification by Hybrid Capture |
| US20030208196A1 (en) * | 2002-05-03 | 2003-11-06 | Arthrocare Corporation | Control system for limited-use device |
| US7648678B2 (en) * | 2002-12-20 | 2010-01-19 | Dako Denmark A/S | Method and system for pretreatment of tissue slides |
| US20040185484A1 (en) * | 2003-01-29 | 2004-09-23 | Costa Gina L. | Method for preparing single-stranded DNA libraries |
| EP1613771B1 (en) | 2003-04-04 | 2012-03-21 | Roche Diagnostics GmbH | Improved system for multi color real time pcr |
| US7129260B2 (en) | 2003-06-02 | 2006-10-31 | Abbott Laboratories | Isoindolinone kinase inhibitors |
| JP5183063B2 (ja) | 2003-07-05 | 2013-04-17 | ザ ジョンズ ホプキンス ユニバーシティ | 遺伝的変異の検出および列挙のための方法ならびに組成物 |
| DE60300339T2 (de) * | 2003-08-25 | 2006-04-13 | Mtm Laboratories Ag | Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben |
| WO2005033333A2 (en) | 2003-10-07 | 2005-04-14 | Dako Denmark A/S | Methods and compositions for the diagnosis of cancer |
| US8012944B2 (en) | 2003-10-30 | 2011-09-06 | Pharmascience Inc. | Method for treating cancer using IAP antisense oligomer and chemotherapeutic agent |
| US20070083286A1 (en) * | 2004-03-09 | 2007-04-12 | Olympus Corporation | Medical instrument and equipment for managing of the same |
| CN1690223B (zh) | 2004-04-27 | 2010-05-05 | 亚能生物技术（深圳）有限公司 | 人乳头瘤病毒分型基因芯片检测系统 |
| US7398181B2 (en) * | 2004-06-02 | 2008-07-08 | Hewlett-Packard Development Company, L.P. | Method for retrieving reliability data in a system |
| US20060095096A1 (en) * | 2004-09-09 | 2006-05-04 | Debenedictis Leonard C | Interchangeable tips for medical laser treatments and methods for using same |
| EP1794319B1 (en) | 2004-09-30 | 2014-08-13 | Epigenomics AG | Method for providing dna fragments derived from an archived sample |
| WO2006050166A2 (en) * | 2004-10-29 | 2006-05-11 | Medimmune, Inc. | Methods of preventing and treating rsv infections and related conditions |
| US20060129269A1 (en) * | 2004-12-15 | 2006-06-15 | Xerox Corporation | Processes for using a memory storage device in conjunction with tooling |
| AU2006205638A1 (en) | 2005-01-14 | 2006-07-20 | The Regents Of The University Of Michigan | Systems, methods, and compositions for detection of human papilloma virus in biological samples |
| US20060240449A1 (en) | 2005-01-19 | 2006-10-26 | Mcglennen Ronald C | Methods and compositions for preparation of biological samples |
| CA2602185A1 (en) | 2005-04-06 | 2006-10-06 | Verenium Corporation | Enzymes and formulations for broad-specificity decontamination of chemical and biological warfare agents |
| US7972776B2 (en) * | 2005-11-15 | 2011-07-05 | Oncohealth Corporation | Protein chips for HPV detection |
| US20080003565A1 (en) | 2006-05-02 | 2008-01-03 | Government Of The Us, As Represented By The Secretary, Department Of Health And Human Services | Viral nucleic acid microarray and method of use |
| WO2007134252A1 (en) | 2006-05-11 | 2007-11-22 | Becton, Dickinson And Company | Method of protein extraction from cells |
| EP2413142B1 (en) | 2007-02-27 | 2013-06-05 | Nuclea Biomarkers LLC | Method for predicting the response of NSCLC-patients to treatment by an EGFR-TK inhibitor |
| US7985375B2 (en) | 2007-04-06 | 2011-07-26 | Qiagen Gaithersburg, Inc. | Sample preparation system and method for processing clinical specimens |
| US8357538B2 (en) | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
| US8703492B2 (en) | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
| WO2008139938A1 (ja) | 2007-05-02 | 2008-11-20 | National University Corporation Tokyo Medical And Dental University | ヒトパピローマウイルス１６型遺伝子を標的とする二本鎖核酸分子及びそれを含む医薬 |
| LT3101141T (lt) | 2007-06-08 | 2020-03-10 | Epigenomics Ag | Metilinimo analizės būdas |
| CN101177701B (zh) | 2007-09-29 | 2010-08-18 | 潮州凯普生物化学有限公司 | 人乳头状瘤病毒基因分型检测试剂盒及其基因芯片制备方法 |
| AU2008310704B2 (en) | 2007-10-11 | 2014-03-20 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods and compositions for the diagnosis and treatment of esphageal adenocarcinomas |
| JP2009106220A (ja) | 2007-10-31 | 2009-05-21 | Genodive Pharma Kk | 基板上での等温増幅反応による標的塩基配列の捕捉及び検出方法 |
| PT2209920T (pt) | 2007-11-01 | 2016-08-02 | Self-Screen B V | Método inovador de deteção para hpvs cervicais |
| US20110027798A1 (en) | 2008-03-31 | 2011-02-03 | The Ohio State University Research Foundation | Hybridization Quantitation Method for Modified Micro-RNA and -DNA Based Oligonucleotides |
| JP2011518333A (ja) | 2008-04-17 | 2011-06-23 | キアジェン ゲイサーズバーグ インコーポレイテッド | 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット |
| WO2010004251A1 (en) | 2008-06-16 | 2010-01-14 | Oncomethylome Sciences Sa | Dna methylomes |
| US20100121046A1 (en) | 2008-09-05 | 2010-05-13 | Mayo Foundation For Medical Education And Research | Collecting and processing complex macromolecular mixtures |
| US9090948B2 (en) | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
| AU2009320213B2 (en) | 2008-10-27 | 2016-06-16 | Qiagen Gaithersburg Inc. | Fast results hybrid capture assay and system |
| EP2184368A1 (en) | 2008-11-07 | 2010-05-12 | DKFZ Deutsches Krebsforschungszentrum | Diagnostic transcript and splice patterns of HPV16 in different cervical lesions |
| EP2391733A1 (en) * | 2009-01-27 | 2011-12-07 | QIAGEN Gaithersburg, Inc. | Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection |
| EP2385979B1 (en) * | 2009-01-28 | 2017-08-16 | QIAGEN Gaithersburg, Inc. | Sequence-specific large volume sample preparation method and assay |
| EP2425019B1 (en) | 2009-05-01 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
| US20110053143A1 (en) * | 2009-09-03 | 2011-03-03 | Qiagen Gaithersburg Inc. | Methods and kits for determining viral load in clinical samples |
| EP2478087B1 (en) * | 2009-09-14 | 2017-01-18 | QIAGEN Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| JP5876834B2 (ja) * | 2010-01-04 | 2016-03-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 固定された組織サンプルからの核酸またはタンパク質の回収のための方法、組成物、およびキット |
| CA2786473A1 (en) * | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| BR112012018545A2 (pt) * | 2010-01-29 | 2016-05-03 | Qiagen Gaithersburg Inc | método de determinação e confirmação da presença de um hpv em uma amostra |
| WO2011094514A1 (en) * | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| AU2011255638B2 (en) * | 2010-05-19 | 2016-08-25 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| AU2011258501B2 (en) * | 2010-05-25 | 2016-07-07 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically-truncated probes |
-
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- 2009-10-26 AU AU2009320213A patent/AU2009320213B2/en not_active Ceased
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| US8735564B2 (en) | 2014-05-27 |
| AU2009320213A1 (en) | 2010-06-03 |
| TWI419974B (zh) | 2013-12-21 |
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