SI2281895T1 - Postopki za izboljšanje tolerance na stres v rastlinah in sestavki za njih - Google Patents

Postopki za izboljšanje tolerance na stres v rastlinah in sestavki za njih Download PDF

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SI2281895T1
SI2281895T1 SI200432434T SI200432434T SI2281895T1 SI 2281895 T1 SI2281895 T1 SI 2281895T1 SI 200432434 T SI200432434 T SI 200432434T SI 200432434 T SI200432434 T SI 200432434T SI 2281895 T1 SI2281895 T1 SI 2281895T1
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sequence
transgenic
plant
cold
seq
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SI200432434T
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Mary Fernandes
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Monsanto Technology, Llc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • Plant Pathology (AREA)
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  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (11)

  1. Postopki za izboljšanje tolerance na stres v rastlinah in sestavki za njih Patentni zahtevki
    1. Transgeno seme, ki v svojem genomu vsebuje molekulo rekombinantne DNA, ki kodira protein hladnega šoka, ki ima vsaj 90% enakosti z zaporedjem SEQ ID NO: 63 ali 65, je kjer omenjeno zaporedje domene hladnega šoka, ki vsebuje protein hladnega šoka [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] zaporedja SEQ ID NO:3 in ima vezavno funkcijo eno vijačne nukleinske kisline, kjer molekula rekombinantne DNA izraža protein hladnega šoka v transgeni rastlini, gojeni iz semena, kjer ima transgena rastlina višjo stopnjo rasti pri pogojih, kjer je voda za rast omejena za ne-transformirano rastlino iste vrste ali, kjer je transgena rastlina odporna na sušo, v primeijavi z ne- transformirano rastlino iste vrste.
  2. 2. Seme po zahtevku 1, kjer je omenjeno seme iz transgene rastline soje, koruze, oljne repice, riža, bombaža, ječmena, ovsa, tratne trave, bombaža ali pšenice.
  3. 3. Seme po zahtevku 1 ali 2, kjer ima omenjeni protein hladnega šoka zaporedje amino kislin, kije enako zaporedju SEQ ID NO: 63 ali 65.
  4. 4. Postopek izdelave transgenih rastlin odpornih na sušo, ki obsega naslednje korake: (a) vstavljanje v genom rastlinske celic rekombinantne DNA, ki vsebuje, v smeri 5' do 3': prvi polinukleotid DNA, ki vsebuje promotor, ki deluje v rastlinah in, kije operativno povezan z drugim polinukleotidom DNA, ki protein hladnega šoka, ki ima vsaj 90% enakosti z zaporedjem SEQ ID NO: 63 ali 65, kjer je omenjeno zaporedje domene hladnega šoka, ki vsebuje protein hladnega šoka [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] zaporedja SEQ ID NO:3 in ima vezavno funkcijo eno vijačne nukleinske kisline, operativno povezano s polinukleotidom DNA 3' transkripcijskega zaključka, ki deluje kot poliadenilacijsko zaporedje; (b) pridobivanje transformirane rastlinske celice, ki vsebuje omenjeno rekombinantno DNA; (c) regeneriranje rastlin iz omenjenih rastlinskih celic; in (d) izbiro rastline za povišano odpornost na sušo.
  5. 5. Postopek po zahtevku 4, kjer je omenjena rastlina transgena rastlina soje, koruze, oljne repice, riža, bombaža, ječmena, ovsa, tratne trave, bombaža ali pšenice.
  6. 6. Postopek po zahtevku 4 ali 5, kjer ima omenjeni protein hladnega šoka zaporedje amino kislin, kije enako zaporedju SEQ ID NO: 63 ali 65.
  7. 7. Transgena propagula, ki v svojem genomu vsebuje molekulo rekombinantne DNA, ki kodira protein hladnega šoka, ki ima vsaj 90% enakosti z zaporedjem SEQ ID NO: 63 ali 65, kjer je omenjeno zaporedje domene hladnega šoka, ki vsebuje protein hladnega šoka [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] zaporedja SEQ ID NO:3 in ima vezavno funkcijo eno vijačne nukleinske kisline, kjer molekula rekombinantne DNA izraža protein hladnega šoka v rastlinah, gojenih iz propagule, kjer omenjene rastline kažejo odpornost na sušo v primerjavi z ne- transformirano rastlino iste vrste.
  8. 8. Transgena propagula po zahtevku 7, kjer je omenjena transgena propagula iz rastline transgene soje, koruze, oljne repice, riža, bombaža, ječmena, ovsa, tratne trave, bombaža ali pšenice.
  9. 9. Transgena propagula po zahtevku 7 ali 8, kjer ima omenjeni protein hladnega šoka zaporedje amino kislin, kije enako zaporedju SEQ ID NO: 63 ali 65.
  10. 10. Uporaba semen po zahtevku 1, za pridobivanja polja transgenih rastlin, ki so odporne na sušo.
  11. 11. Uporaba propagul po zahtevku 7, za pridobivanja polja transgenih rastlin, ki so odporne na sušo.
SI200432434T 2003-09-29 2004-09-29 Postopki za izboljšanje tolerance na stres v rastlinah in sestavki za njih SI2281895T1 (sl)

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US50671703P 2003-09-29 2003-09-29
US53045303P 2003-12-17 2003-12-17
EP10185254.9A EP2281895B1 (en) 2003-09-29 2004-09-29 Methods for enhancing stress tolerance in plants and compositions thereof

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EP (3) EP1668141B1 (sl)
JP (2) JP4864710B2 (sl)
KR (2) KR101077334B1 (sl)
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AU (1) AU2004278752B2 (sl)
BR (2) BRPI0414880B1 (sl)
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