SI20644B - Ekspresijski sistem za faktor viii - Google Patents
Ekspresijski sistem za faktor viii Download PDFInfo
- Publication number
- SI20644B SI20644B SI9920097A SI9920097A SI20644B SI 20644 B SI20644 B SI 20644B SI 9920097 A SI9920097 A SI 9920097A SI 9920097 A SI9920097 A SI 9920097A SI 20644 B SI20644 B SI 20644B
- Authority
- SI
- Slovenia
- Prior art keywords
- protein
- factor viii
- cells
- crl
- expressed
- Prior art date
Links
- 229960000301 factor viii Drugs 0.000 title claims 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract 13
- 102000004169 proteins and genes Human genes 0.000 claims abstract 13
- 238000000034 method Methods 0.000 claims abstract 7
- 210000004027 cell Anatomy 0.000 claims abstract 6
- 108010054218 Factor VIII Proteins 0.000 claims abstract 4
- 102000001690 Factor VIII Human genes 0.000 claims abstract 4
- 210000005260 human cell Anatomy 0.000 claims abstract 4
- 238000004519 manufacturing process Methods 0.000 claims abstract 2
- 108091026890 Coding region Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 108010089996 B-domain-deleted factor VIII Proteins 0.000 abstract 1
- 230000006978 adaptation Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000009795 derivation Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/028—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a herpesvirus
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- Biomedical Technology (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Ta izum opisuje proizvodni postopek brez proteinov za proteine, ki imajo prokoagulatno aktivnost faktorja VIII. Splošno postopek vključuje derivacijo stabilnih humanih celičnih klonov z visoko produktivnostjo za faktor VIII z deletirano B-domeno in (2) prilagoditev celic na rast v mediju brez plazemsko izvedenih proteinov. Bolj natančno postopek vključuje transfekcijo humanih celic z vektorjem, kot je na sliki, ki obsega selektibilni marker in sekvenco, ki kodira protein, ki ima prokoagulantno aktivnost faktorja VIII, selekcioniranje celic s selekcijskim sredstvom in izoliranje klonov, ki izražajo visoke nivoje proteina, ki ima prokoagulantno aktivnost faktorja VIII.
Claims (8)
1 Patentni zahtevki 1. Postopek proizvodnje in izoliranja proteina, ki ima aktivnost faktoija VIII, ki obsega gojenje celic, imenovanih pri American Type Culture Collection kot CRL-12568, ki vključujejo sekvenco, ki kodira za protein, operabilno vezano na promotor, pri čemer gojenje poteka pod pogoji, ki so dovoljšnji, da se izrazi protein, in izoliranje proteina.
2. Postopek po zahtevku 1, označen s tem, da ima protein aminokislinsko sekvenco imenovano kot SEKV ID ŠT.: 1.
3. Postopek po zahtevku 2, označen s tem, da se protein izrazi na nivoju vsaj 2 pU/c/d, kadar celice gojimo v iz plazme izvirajočem mediju brez proteinov.
4. Postopek po zahtevku 3, kjer se protein izrazi na nivoju vsaj 4 pU/c/d.
5. Postopek po zahtevku 4, kjer se protein izrazi na nivoju vsaj 5 pU/c/d.
6. Humana celična linija, izvedena iz celic, imenovanih pri American Type Culture Collection kot CRL-12568, z vključitvijo sekvence, ki kodira za protein, ki ima aktivnost faktoija VIII, operabilno vezane na promotor.
7. Humana celična linija po zahtevku 6, ki izraža faktor VIII z deletirano B-domeno.
8. Celična linija, imenovana pri American Type Culture Collection kot CRL-12582.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/209,916 US6358703B1 (en) | 1998-12-10 | 1998-12-10 | Expression system for factor VIII |
| PCT/US1999/029169 WO2000034505A1 (en) | 1998-12-10 | 1999-12-08 | Expression system for factor viii |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SI20644A SI20644A (sl) | 2002-02-28 |
| SI20644B true SI20644B (sl) | 2009-04-30 |
Family
ID=22780858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI9920097A SI20644B (sl) | 1998-12-10 | 1999-12-08 | Ekspresijski sistem za faktor viii |
Country Status (26)
| Country | Link |
|---|---|
| US (10) | US6358703B1 (sl) |
| EP (1) | EP1137797B1 (sl) |
| JP (1) | JP4240818B2 (sl) |
| KR (1) | KR100616028B1 (sl) |
| AT (1) | ATE412765T1 (sl) |
| AU (1) | AU761801B2 (sl) |
| BG (1) | BG65431B1 (sl) |
| BR (1) | BRPI9916069B8 (sl) |
| CA (1) | CA2354845C (sl) |
| CZ (1) | CZ302330B6 (sl) |
| DE (1) | DE69939839D1 (sl) |
| DK (1) | DK1137797T3 (sl) |
| ES (1) | ES2315026T3 (sl) |
| HU (1) | HU228489B1 (sl) |
| IL (2) | IL143353A0 (sl) |
| NO (1) | NO329544B1 (sl) |
| NZ (1) | NZ512234A (sl) |
| PL (1) | PL200676B1 (sl) |
| PT (1) | PT1137797E (sl) |
| RO (1) | RO121604B1 (sl) |
| RU (1) | RU2249041C2 (sl) |
| SI (1) | SI20644B (sl) |
| SK (1) | SK286945B6 (sl) |
| TR (1) | TR200101592T2 (sl) |
| UA (1) | UA77383C2 (sl) |
| WO (1) | WO2000034505A1 (sl) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
| US6180108B1 (en) * | 1998-12-10 | 2001-01-30 | Bayer Corporation | Vectors having terminal repeat sequence of Epstein-Barr virus |
| US6358703B1 (en) * | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
| DE60115613T2 (de) * | 2000-03-22 | 2006-08-24 | Octagene Gmbh | Herstellung von rekombinanten muteine des blutgerinnungsfaktors viii in humanen zellinien |
| EP2110385A1 (en) | 2001-06-14 | 2009-10-21 | The Scripps Research Institute | Stabilized factor VIII with engineered disulfide bonds |
| WO2004075923A2 (en) * | 2003-02-26 | 2004-09-10 | Nektar Therapeutics Al, Corporation | Polymer-factor viii moiety conjugates |
| US7485291B2 (en) * | 2003-06-03 | 2009-02-03 | Cell Genesys, Inc. | Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof |
| WO2005017149A1 (en) * | 2003-06-03 | 2005-02-24 | Cell Genesys, Inc. | Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site |
| EP1812557A4 (en) * | 2004-10-29 | 2009-11-04 | Centocor Ortho Biotech Inc | COMPOSITIONS OF CHEMICALLY DEFINED MEDIA |
| ES2633916T3 (es) | 2004-11-12 | 2017-09-26 | Bayer Healthcare Llc | Modificación de FVIII dirigida al sitio |
| JP5096369B2 (ja) | 2005-12-20 | 2012-12-12 | ブリストル−マイヤーズ スクイブ カンパニー | 組成物および組成物の製造方法 |
| AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
| PT2574677T (pt) * | 2007-12-27 | 2017-10-19 | Baxalta Inc | Processos para cultura de células |
| KR101005967B1 (ko) * | 2008-04-12 | 2011-01-05 | (주)셀트리온 | 우수한 재조합 단백질을 생산하기 위한 인간 숙주 세포 |
| CN102137935A (zh) * | 2008-06-25 | 2011-07-27 | 拜耳医药保健有限公司 | 具有降低的免疫原性的因子viii突变蛋白 |
| EP2331569A1 (en) | 2008-08-21 | 2011-06-15 | Octapharma AG | Recombinantly produced human factor viii and ix |
| AU2009289212B2 (en) * | 2008-09-03 | 2015-02-12 | Octapharma Ag | New protecting compositions for recombinantly produced factor VIII |
| EA020843B1 (ru) | 2009-02-03 | 2015-02-27 | Амуникс Оперейтинг Инк. | Удлиненные рекомбинантные полипептиды и содержащие их композиции |
| JP5739865B2 (ja) * | 2009-03-24 | 2015-06-24 | バイエル・ヘルスケア・エルエルシー | 第ｖｉｉｉ因子変異体および使用の方法 |
| US20120263701A1 (en) | 2009-08-24 | 2012-10-18 | Volker Schellenberger | Coagulation factor vii compositions and methods of making and using same |
| US20110150843A1 (en) * | 2009-10-30 | 2011-06-23 | National Institute Of Immunology | Method for the therapeutic correction of hemophilia a by transplanting bone marrow cells |
| US20130017997A1 (en) * | 2010-08-19 | 2013-01-17 | Amunix Operating Inc. | Factor VIII Compositions and Methods of Making and Using Same |
| EP2707387B1 (en) * | 2011-05-13 | 2019-12-25 | Octapharma AG | A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii |
| CN104271150A (zh) | 2012-01-12 | 2015-01-07 | 比奥根艾迪克Ma公司 | 嵌合因子viii多肽及其用途 |
| MX369862B (es) | 2012-02-15 | 2019-11-25 | Bioverativ Therapeutics Inc | Composiciones del factor viii y metodos para hacerlas y usarlas. |
| NZ628014A (en) | 2012-02-15 | 2016-09-30 | Biogen Ma Inc | Recombinant factor viii proteins |
| WO2017180807A1 (en) | 2016-04-15 | 2017-10-19 | Baxalta Incorporated | Method and apparatus for providing a pharmacokinetic drug dosing regiment |
| EP3033097B1 (en) | 2013-08-14 | 2021-03-10 | Bioverativ Therapeutics Inc. | Factor viii-xten fusions and uses thereof |
| PT4176894T (pt) | 2014-01-10 | 2024-05-21 | Bioverativ Therapeutics Inc | Proteínas quiméricas de fator viii e utilizações destas |
| CA2994547A1 (en) | 2015-08-03 | 2017-02-09 | Bioverativ Therapeutics Inc. | Factor ix fusion proteins and methods of making and using same |
| KR102580477B1 (ko) | 2016-07-22 | 2023-09-19 | 넥타르 테라퓨틱스 | 옥심-함유 결합을 갖는 인자 viii 모이어티의 컨쥬게이트 |
| WO2018102743A1 (en) | 2016-12-02 | 2018-06-07 | Bioverativ Therapeutics Inc. | Methods of treating hemophilic arthropathy using chimeric clotting factors |
| US10896749B2 (en) | 2017-01-27 | 2021-01-19 | Shire Human Genetic Therapies, Inc. | Drug monitoring tool |
| WO2019055717A1 (en) * | 2017-09-13 | 2019-03-21 | Evolve Biosystems, Inc. | OLIGOSACCHARIDE COMPOSITIONS AND THEIR USE DURING THE TRANSIENT PHASES OF INTESTINAL MICROBIOMA OF A MAMMALIAN |
| KR20190086269A (ko) * | 2018-01-12 | 2019-07-22 | 재단법인 목암생명과학연구소 | 체내 지속형 재조합 당단백질 및 이의 제조방법 |
| SG11202010767SA (en) | 2018-05-18 | 2020-11-27 | Bioverativ Therapeutics Inc | Methods of treating hemophilia a |
| CA3115535A1 (en) * | 2018-10-23 | 2020-04-30 | The Children's Hospital Of Philadelphia | Compositions and methods for modulating factor viii function |
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| US4970300A (en) * | 1985-02-01 | 1990-11-13 | New York University | Modified factor VIII |
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| US5661008A (en) * | 1991-03-15 | 1997-08-26 | Kabi Pharmacia Ab | Recombinant human factor VIII derivatives |
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| ATE272122T1 (de) * | 1993-06-10 | 2004-08-15 | Bayer Ag | Vektoren und zelllinien von säugetieren mit erhöhter produktivität |
| US5952198A (en) * | 1995-05-04 | 1999-09-14 | Bayer Corporation | Production of recombinant Factor VIII in the presence of liposome-like substances of mixed composition |
| DE19517194A1 (de) * | 1995-05-11 | 1996-11-14 | Giesecke & Devrient Gmbh | Vorrichtung und Verfahren zur Prüfung von Blattgut, wie z.B. Banknoten oder Wertpapiere |
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| US5923413A (en) * | 1996-11-15 | 1999-07-13 | Interbold | Universal bank note denominator and validator |
| US5804420A (en) * | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| AU735763B2 (en) * | 1997-12-05 | 2001-07-12 | Immune Response Corporation, The | Novel vectors and genes exhibiting increased expression |
| US6040584A (en) * | 1998-05-22 | 2000-03-21 | Mti Corporation | Method and for system for detecting damaged bills |
| US6528286B1 (en) * | 1998-05-29 | 2003-03-04 | Genentech, Inc. | Mammalian cell culture process for producing glycoproteins |
| US6358703B1 (en) * | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
| US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
| US6180108B1 (en) * | 1998-12-10 | 2001-01-30 | Bayer Corporation | Vectors having terminal repeat sequence of Epstein-Barr virus |
-
1998
- 1998-12-10 US US09/209,916 patent/US6358703B1/en not_active Expired - Lifetime
-
1999
- 1999-08-12 UA UA2001074777A patent/UA77383C2/uk unknown
- 1999-12-08 DE DE69939839T patent/DE69939839D1/de not_active Expired - Lifetime
- 1999-12-08 PT PT99966068T patent/PT1137797E/pt unknown
- 1999-12-08 SI SI9920097A patent/SI20644B/sl active Search and Examination
- 1999-12-08 RU RU2001119156/13A patent/RU2249041C2/ru active
- 1999-12-08 WO PCT/US1999/029169 patent/WO2000034505A1/en not_active Ceased
- 1999-12-08 CZ CZ20012024A patent/CZ302330B6/cs not_active IP Right Cessation
- 1999-12-08 TR TR2001/01592T patent/TR200101592T2/xx unknown
- 1999-12-08 JP JP2000586938A patent/JP4240818B2/ja not_active Expired - Lifetime
- 1999-12-08 SK SK792-2001A patent/SK286945B6/sk not_active IP Right Cessation
- 1999-12-08 HU HU0200558A patent/HU228489B1/hu unknown
- 1999-12-08 DK DK99966068T patent/DK1137797T3/da active
- 1999-12-08 NZ NZ512234A patent/NZ512234A/xx not_active IP Right Cessation
- 1999-12-08 BR BRPI9916069A patent/BRPI9916069B8/pt not_active IP Right Cessation
- 1999-12-08 AT AT99966068T patent/ATE412765T1/de active
- 1999-12-08 CA CA002354845A patent/CA2354845C/en not_active Expired - Lifetime
- 1999-12-08 IL IL14335399A patent/IL143353A0/xx unknown
- 1999-12-08 RO ROA200100649A patent/RO121604B1/ro unknown
- 1999-12-08 KR KR1020017007149A patent/KR100616028B1/ko not_active Expired - Lifetime
- 1999-12-08 EP EP99966068A patent/EP1137797B1/en not_active Expired - Lifetime
- 1999-12-08 AU AU21701/00A patent/AU761801B2/en not_active Expired
- 1999-12-08 PL PL349284A patent/PL200676B1/pl unknown
- 1999-12-08 ES ES99966068T patent/ES2315026T3/es not_active Expired - Lifetime
-
2001
- 2001-05-24 IL IL143353A patent/IL143353A/en not_active IP Right Cessation
- 2001-06-01 NO NO20012718A patent/NO329544B1/no not_active IP Right Cessation
- 2001-06-06 BG BG105567A patent/BG65431B1/bg unknown
- 2001-12-06 US US10/006,091 patent/US20020102730A1/en not_active Abandoned
-
2002
- 2002-01-15 US US10/047,257 patent/US20020115152A1/en not_active Abandoned
- 2002-08-22 US US10/225,900 patent/US7459525B2/en not_active Expired - Fee Related
-
2007
- 2007-04-25 US US11/789,580 patent/US20090036358A1/en not_active Abandoned
-
2010
- 2010-11-08 US US12/941,685 patent/US8207117B2/en not_active Expired - Fee Related
-
2012
- 2012-05-29 US US13/482,326 patent/US8945869B2/en not_active Expired - Fee Related
-
2013
- 2013-06-14 US US13/918,053 patent/US9249209B2/en not_active Expired - Fee Related
-
2015
- 2015-12-30 US US14/985,134 patent/US9650431B2/en not_active Expired - Fee Related
-
2017
- 2017-04-05 US US15/480,162 patent/US20170267745A1/en not_active Abandoned
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