SG192545A1 - Isoprene synthase variants for improved microbial production of isoprene - Google Patents

Isoprene synthase variants for improved microbial production of isoprene Download PDF

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Publication number
SG192545A1
SG192545A1 SG2013055827A SG2013055827A SG192545A1 SG 192545 A1 SG192545 A1 SG 192545A1 SG 2013055827 A SG2013055827 A SG 2013055827A SG 2013055827 A SG2013055827 A SG 2013055827A SG 192545 A1 SG192545 A1 SG 192545A1
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SG
Singapore
Prior art keywords
isoprene
isoprene synthase
variant
polypeptide
seq
Prior art date
Application number
SG2013055827A
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English (en)
Inventor
Marguerite A Cervin
Gregory M Whited
Andrei Miasnikov
Caroline M Peres
Walter Weyler
Derek H Wells
Richard R Bott
Christopher Lee Rife
Joseph C Mcauliffe
James Kellis
Original Assignee
Danisco Us Inc
Goodyear Tire & Rubber
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc, Goodyear Tire & Rubber filed Critical Danisco Us Inc
Publication of SG192545A1 publication Critical patent/SG192545A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG2013055827A 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene SG192545A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12533608P 2008-04-23 2008-04-23

Publications (1)

Publication Number Publication Date
SG192545A1 true SG192545A1 (en) 2013-08-30

Family

ID=40810540

Family Applications (2)

Application Number Title Priority Date Filing Date
SG2013055827A SG192545A1 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene
SG201007802A SG165884A1 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG201007802A SG165884A1 (en) 2008-04-23 2009-04-23 Isoprene synthase variants for improved microbial production of isoprene

Country Status (14)

Country Link
US (3) US8173410B2 (https=)
EP (1) EP2279251A2 (https=)
JP (2) JP2011518564A (https=)
KR (1) KR20110020234A (https=)
CN (1) CN102171335A (https=)
AU (1) AU2009240505B2 (https=)
BR (1) BRPI0911667A2 (https=)
CA (1) CA2722440A1 (https=)
MX (1) MX2010011706A (https=)
MY (1) MY153871A (https=)
RU (2) RU2516343C2 (https=)
SG (2) SG192545A1 (https=)
WO (1) WO2009132220A2 (https=)
ZA (2) ZA201007564B (https=)

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