EP1311709A4 - Methods and compositions for directed molecular evolution using dna-end modification - Google Patents

Methods and compositions for directed molecular evolution using dna-end modification

Info

Publication number
EP1311709A4
EP1311709A4 EP01965982A EP01965982A EP1311709A4 EP 1311709 A4 EP1311709 A4 EP 1311709A4 EP 01965982 A EP01965982 A EP 01965982A EP 01965982 A EP01965982 A EP 01965982A EP 1311709 A4 EP1311709 A4 EP 1311709A4
Authority
EP
European Patent Office
Prior art keywords
dna
compositions
methods
molecular evolution
end modification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01965982A
Other languages
German (de)
French (fr)
Other versions
EP1311709A1 (en
Inventor
Vaughn Smider
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integrigen Inc
Original Assignee
Integrigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrigen Inc filed Critical Integrigen Inc
Publication of EP1311709A1 publication Critical patent/EP1311709A1/en
Publication of EP1311709A4 publication Critical patent/EP1311709A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
EP01965982A 2000-08-18 2001-08-17 Methods and compositions for directed molecular evolution using dna-end modification Withdrawn EP1311709A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22647700P 2000-08-18 2000-08-18
US226477P 2000-08-18
PCT/US2001/025788 WO2002016642A1 (en) 2000-08-18 2001-08-17 Methods and compositions for directed molecular evolution using dna-end modification

Publications (2)

Publication Number Publication Date
EP1311709A1 EP1311709A1 (en) 2003-05-21
EP1311709A4 true EP1311709A4 (en) 2006-08-02

Family

ID=22849054

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01965982A Withdrawn EP1311709A4 (en) 2000-08-18 2001-08-17 Methods and compositions for directed molecular evolution using dna-end modification

Country Status (5)

Country Link
EP (1) EP1311709A4 (en)
JP (1) JP2004507247A (en)
AU (1) AU2001286528A1 (en)
CA (1) CA2419961A1 (en)
WO (1) WO2002016642A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG192545A1 (en) * 2008-04-23 2013-08-30 Danisco Us Inc Isoprene synthase variants for improved microbial production of isoprene
US9108194B2 (en) * 2010-09-24 2015-08-18 Covaris, Inc. Method and apparatus for fragmenting nucleic acids

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIONDI RICHARD M ET AL: "Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 21, 1 November 1998 (1998-11-01), pages 4946 - 4952, XP002187520, ISSN: 0305-1048 *
CRAMERI A ET AL: "DNA SHUFFLING OF A FAMILY OF GENES FROM DIVERSE SPECIES ACCELERATESDIRECTED EVOLUTION", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 391, January 1998 (1998-01-01), pages 288 - 291, XP000919283, ISSN: 0028-0836 *
KIM ET AL: "Directed Evolution and Identification of Control Regions of ColE1 Plasmid Replication Origins Using Only Nucleotide Deletions", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 351, no. 4, 26 August 2005 (2005-08-26), pages 763 - 775, XP005004656, ISSN: 0022-2836 *
OSTERMEIER M ET AL: "A combinatorial approach to hybrid enzymes independent of DNA homology.", NATURE BIOTECHNOLOGY. DEC 1999, vol. 17, no. 12, December 1999 (1999-12-01), pages 1205 - 1209, XP002179317, ISSN: 1087-0156 *
See also references of WO0216642A1 *

Also Published As

Publication number Publication date
AU2001286528A1 (en) 2002-03-04
CA2419961A1 (en) 2002-02-28
JP2004507247A (en) 2004-03-11
EP1311709A1 (en) 2003-05-21
WO2002016642A9 (en) 2003-03-27
WO2002016642A1 (en) 2002-02-28

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20030318

AK Designated contracting states

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

A4 Supplementary search report drawn up and despatched

Effective date: 20060630

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20060929