SG10202104816QA - Methods and compositions for analyzing cellular components - Google Patents

Methods and compositions for analyzing cellular components

Info

Publication number
SG10202104816QA
SG10202104816QA SG10202104816QA SG10202104816QA SG10202104816QA SG 10202104816Q A SG10202104816Q A SG 10202104816QA SG 10202104816Q A SG10202104816Q A SG 10202104816QA SG 10202104816Q A SG10202104816Q A SG 10202104816QA SG 10202104816Q A SG10202104816Q A SG 10202104816QA
Authority
SG
Singapore
Prior art keywords
compositions
methods
cellular components
analyzing cellular
analyzing
Prior art date
Application number
SG10202104816QA
Other languages
English (en)
Inventor
Kevin L Gunderson
Frank J Steemers
Jeffrey S Fisher
Roberto Rigatti
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG10202104816QA publication Critical patent/SG10202104816QA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10202104816QA 2015-02-10 2016-02-10 Methods and compositions for analyzing cellular components SG10202104816QA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562114505P 2015-02-10 2015-02-10

Publications (1)

Publication Number Publication Date
SG10202104816QA true SG10202104816QA (en) 2021-06-29

Family

ID=55587329

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201706504RA SG11201706504RA (en) 2015-02-10 2016-02-10 Methods and compositions for analyzing cellular components
SG10202104816QA SG10202104816QA (en) 2015-02-10 2016-02-10 Methods and compositions for analyzing cellular components

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201706504RA SG11201706504RA (en) 2015-02-10 2016-02-10 Methods and compositions for analyzing cellular components

Country Status (14)

Country Link
US (2) US11634707B2 (fr)
EP (2) EP3725893A1 (fr)
JP (1) JP7001475B2 (fr)
KR (3) KR20170135834A (fr)
CN (3) CN117106871A (fr)
AU (2) AU2016219328B2 (fr)
BR (1) BR112017017025B1 (fr)
CA (2) CA2975739C (fr)
DK (1) DK3256604T3 (fr)
ES (1) ES2786652T3 (fr)
PT (1) PT3256604T (fr)
RU (2) RU2761432C2 (fr)
SG (2) SG11201706504RA (fr)
WO (1) WO2016130704A2 (fr)

Families Citing this family (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2697397T3 (pl) 2011-04-15 2017-08-31 The Johns Hopkins University System bezpiecznego sekwencjonowania
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11525163B2 (en) 2012-10-29 2022-12-13 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
WO2014093676A1 (fr) 2012-12-14 2014-06-19 10X Technologies, Inc. Procédés et systèmes pour le traitement de polynucléotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102190198B1 (ko) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
DK3102722T3 (da) 2014-02-04 2020-11-16 Jumpcode Genomics Inc Genom fraktionering
JP6838969B2 (ja) 2014-06-26 2021-03-03 10エックス ジェノミクス, インコーポレイテッド 個々の細胞または細胞集団由来の核酸の分析方法
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
DK3227684T3 (da) 2014-12-03 2019-11-11 Isoplexis Corp Analyse af og screening for cellesekretionsprofiler
WO2016114970A1 (fr) 2015-01-12 2016-07-21 10X Genomics, Inc. Procédés et systèmes de préparation de librairies de séquençage d'acide nucléique et librairies préparées au moyen de ceux-ci
EP4286516A3 (fr) 2015-02-24 2024-03-06 10X Genomics, Inc. Procédés et systèmes de traitement de cloisonnement
US10968536B2 (en) * 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
WO2017027653A1 (fr) 2015-08-11 2017-02-16 The Johns Hopkins University Analyse du fluide d'un kyste ovarien
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
KR102379048B1 (ko) 2016-05-02 2022-03-28 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
KR102475710B1 (ko) 2016-07-22 2022-12-08 오레곤 헬스 앤드 사이언스 유니버시티 단일 세포 전체 게놈 라이브러리 및 이의 제조를 위한 조합 인덱싱 방법
WO2018057779A1 (fr) * 2016-09-23 2018-03-29 Jianbiao Zheng Compositions de transposons synthétiques et leurs procédés d'utilisation
WO2018064486A1 (fr) 2016-09-29 2018-04-05 Counsyl, Inc. Dépistage prénatal non invasif utilisant une optimisation de profondeur itérative dynamique
JP7348066B2 (ja) * 2016-11-11 2023-09-20 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
WO2018098372A1 (fr) 2016-11-22 2018-05-31 IsoPlexis Corporation Systèmes, dispositifs et procédés de capture de cellules et procédés de fabrication correspondants
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN110431237A (zh) * 2016-12-29 2019-11-08 伊鲁米纳公司 用于在细胞区室中正交接近和标签化生物分子的分析系统
HUE059100T2 (hu) * 2017-01-27 2022-10-28 Integrated Dna Tech Inc Következõ generációs szekvenálási (NGS) könyvtárak létrehozása kompetitív szálhelyettesítéssel
US10894979B2 (en) 2017-01-27 2021-01-19 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
WO2018140966A1 (fr) 2017-01-30 2018-08-02 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
WO2018144217A1 (fr) 2017-01-31 2018-08-09 Counsyl, Inc. Méthodes et compositions pour enrichissement de polynucléotides cibles
US10968447B2 (en) 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
ES2933806T3 (es) * 2017-02-21 2023-02-14 Illumina Inc Tagmentación que usa transposomas inmovilizados con enlazadores
CA3057589A1 (fr) 2017-03-24 2018-09-27 Counsyl, Inc. Appelant de variante de nombre de copies
EP3872187B1 (fr) 2017-04-23 2022-09-21 Illumina Cambridge Limited Compositions et procédés d'amélioration de l'identification d'échantillons dans des bibliothèques indexées d'acides nucléiques
SG11201909916YA (en) 2017-04-23 2019-11-28 Illumina Cambridge Ltd Compositions and methods for improving sample identification in indexed nucleic acid libraries
CA3059839C (fr) 2017-04-23 2023-01-03 Illumina Cambridge Limited Compositions et procedes permettant d'ameliorer l'identification d'echantillons dans des bibliotheques d'acides nucleiques indexes
SG11201909394PA (en) 2017-04-23 2019-11-28 Illumina Inc Compositions and methods for improving sample identification in indexed nucleic acid libraries
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
WO2018203141A1 (fr) 2017-05-05 2018-11-08 Scipio Bioscience Procédés pour piéger et coder par code à barres des unités biologiques discrètes dans un hydrogel
AU2018266377A1 (en) 2017-05-08 2019-11-14 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
CA3064709A1 (fr) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Amplification de marquage d'extremite multiplex d'acides nucleiques
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
AU2018280131A1 (en) 2017-06-07 2020-01-02 Illumina, Inc. Single cell whole genome libraries for methylation sequencing
EP3662482A1 (fr) 2017-07-31 2020-06-10 Illumina Inc. Système de séquençage comportant une agrégation d'échantillons biologiques multiplexés
EP3662083A4 (fr) 2017-08-01 2021-03-03 Illumina, Inc. Indexation spatiale de matériel génétique et préparation de pharmacothèque à l'aide de billes d'hydrogel et de cellules d'écoulement
JP7232476B2 (ja) 2017-08-07 2023-03-08 ザ ジョンズ ホプキンス ユニバーシティ がんを評価及び治療するための方法及び物質
ES2924185T3 (es) 2017-09-25 2022-10-05 Fred Hutchinson Cancer Center Perfiles in situ de alta eficiencia dirigidos a todo el genoma
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
KR102556494B1 (ko) 2017-10-31 2023-07-18 엔코디아, 인코포레이티드 핵산 암호화 및/또는 표지를 이용한 분석용 키트
WO2019099751A1 (fr) 2017-11-15 2019-05-23 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
KR102239487B1 (ko) * 2018-01-08 2021-04-14 일루미나, 인코포레이티드 반도체-기반 검출을 사용한 고-처리율 서열분석
CN112005115A (zh) 2018-02-12 2020-11-27 10X基因组学有限公司 表征来自单个细胞或细胞群体的多种分析物的方法
US11180752B2 (en) 2018-02-13 2021-11-23 Illumina, Inc. DNA sequencing using hydrogel beads
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
AU2019248635B2 (en) 2018-04-02 2022-01-27 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN111051525A (zh) * 2018-04-20 2020-04-21 伊鲁米纳公司 包封单细胞的方法、包封的单细胞及其用途
SG11202011073RA (en) * 2018-05-07 2020-12-30 Bitbiome Inc Method for performing single-cell analysis and device therefor
KR102447811B1 (ko) * 2018-05-17 2022-09-27 일루미나, 인코포레이티드 감소된 증폭 편향을 갖는 고속대량 단일 세포 서열분석
EP4269618A3 (fr) * 2018-06-04 2024-01-10 Illumina, Inc. Procédés de production des bibliothèques de transcriptomes à une seule cellule à haut débit et leurs
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
MX2021003772A (es) 2018-10-26 2021-05-27 Illumina Inc Modulacion de globulos de polimero para procesamiento de adn.
CN109540855B (zh) * 2018-11-07 2021-03-23 沈阳化工大学 一种荧光金壳磁性椭球的制备方法
US20220010373A1 (en) * 2018-11-07 2022-01-13 The University Of Tokyo Method for detecting genome-related information of cell coexisting with at least one type of test substance
EP3887540B1 (fr) * 2018-11-30 2023-08-23 Illumina, Inc. Analyse d'analytes multiples à l'aide d'un seul dosage
AU2019391274A1 (en) 2018-12-05 2021-01-07 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020126602A1 (fr) 2018-12-18 2020-06-25 Illumina Cambridge Limited Procédés et compositions pour séquençage d'extrémités appariées à l'aide d'une amorce de surface unique
SG11202012558VA (en) 2018-12-19 2021-01-28 Illumina Inc Methods for improving polynucleotide cluster clonality priority
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
TW202100247A (zh) 2019-01-29 2021-01-01 美商伊路米納有限公司 流通槽
TW202045709A (zh) 2019-01-29 2020-12-16 美商伊路米納有限公司 流體槽
TW202043486A (zh) 2019-01-29 2020-12-01 美商伊路米納有限公司 定序套組
CN112739459A (zh) 2019-02-04 2021-04-30 伊鲁米纳公司 微流体液滴发生器
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
EP3837365A1 (fr) 2019-03-01 2021-06-23 Illumina, Inc. Banques de noyaux uniques et à cellule unique à haut rendement et leurs procédés de production et d'utilisation
US20220042078A1 (en) 2019-04-29 2022-02-10 Illumina, Inc. Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
AU2020267119A1 (en) 2019-04-30 2021-11-18 Encodia, Inc. Methods for preparing analytes and related kits
CN112689682A (zh) * 2019-05-31 2021-04-20 伊鲁米纳公司 从流动池中的生物样品获取信息
CN111088331B (zh) * 2019-12-16 2023-04-14 中国科学院苏州生物医学工程技术研究所 一种基于压电声波传感器的单分子测序方法
SG11202109486QA (en) 2019-12-19 2021-09-29 Illumina Inc High-throughput single-cell libraries and methods of making and of using
CA3177286A1 (fr) 2020-05-12 2021-11-18 Illumina Inc. Generation d'acides nucleiques avec des bases modifiees au moyen de desoxynucleotidyl transferase terminale recombinante
WO2021252617A1 (fr) 2020-06-09 2021-12-16 Illumina, Inc. Procédés pour augmenter le rendement de bibliothèques de séquençage
KR20230037503A (ko) 2020-06-30 2023-03-16 일루미나, 인코포레이티드 무흔적 dna를 생성하기 위한 촉매적으로 제어된 합성에 의한 서열분석
KR20230038145A (ko) 2020-07-08 2023-03-17 일루미나, 인코포레이티드 트랜스포좀 담체로서의 비드
EP4196600A1 (fr) * 2020-08-14 2023-06-21 Factorial Diagnostics, Inc. Préparation de banques in situ pour le séquençage
KR20230051508A (ko) * 2020-08-18 2023-04-18 일루미나, 인코포레이티드 핵산의 서열-특이적 표적화된 전위 및 선택과 분류
EP4229216A1 (fr) * 2020-10-15 2023-08-23 The Board of Trustees of the Leland Stanford Junior University Détection et analyse de variations structurales dans des génomes
KR102576409B1 (ko) * 2021-01-18 2023-09-08 충남대학교병원 알지네이트 비드 매개의 세포절편 제작방법
WO2022182682A1 (fr) 2021-02-23 2022-09-01 10X Genomics, Inc. Analyse à base de sonde d'acides nucléiques et de protéines
KR102526386B1 (ko) * 2021-03-19 2023-05-02 충남대학교병원 알지네이트와 pva 복합비드를 매개로 하는 세포절편 제작방법
US20220411864A1 (en) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
WO2023086847A1 (fr) 2021-11-10 2023-05-19 Encodia, Inc. Procédés de codage de macromolécules dans des cellules individuelles
CN117813397A (zh) 2021-12-21 2024-04-02 因美纳有限公司 蜡-微球基质组合物及其制备和使用方法
US20230203562A1 (en) 2021-12-28 2023-06-29 Encodia, Inc. High-throughput serotyping and antibody profiling assays
US20230212667A1 (en) 2021-12-29 2023-07-06 Illumina Cambridge Limited Methods of nucleic acid sequencing using surface-bound primers
WO2023196572A1 (fr) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Cytidine désaminases modifiées et méthodes d'utilisation
WO2023209606A1 (fr) 2022-04-29 2023-11-02 Illumina Cambridge Limited Procédés et systèmes d'encapsulation de microsphères lyophilisées
EP4272764A1 (fr) 2022-05-03 2023-11-08 Scipio Bioscience Procédé de complexion d'unités biologiques avec des particules
GB202208728D0 (en) * 2022-06-14 2022-07-27 Cambridge Entpr Ltd Methods for spatial genomic, epigenomic and multi-omic profiling using transposases and light-activated spatial barcoding
US20240003886A1 (en) * 2022-06-15 2024-01-04 Quantum-Si Incorporated Directed protein evolution
WO2024073043A1 (fr) 2022-09-30 2024-04-04 Illumina, Inc. Procédés d'utilisation de protéines de liaison cpg dans la cartographie de nucléotides cytosine modifiés
WO2024069581A1 (fr) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Complexes hélicase-cytidine désaminase et procédés d'utilisation
WO2024073047A1 (fr) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine désaminases et procédés d'utilisation dans la cartographie de nucléotides cytosine modifiés

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
CA2044616A1 (fr) 1989-10-26 1991-04-27 Roger Y. Tsien Sequencage de l'adn
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
CA2256128A1 (fr) 1998-12-29 2000-06-29 Stephen William Davies Traitement d'adn code
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
CN100462433C (zh) 2000-07-07 2009-02-18 维西根生物技术公司 实时序列测定
WO2002007503A1 (fr) 2000-07-25 2002-01-31 The Regents Of The University Of California Micropompage par electromouillage
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
WO2002044425A2 (fr) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Synthese d'acides nucleiques d'enzymes, et compositions et methodes modifiant la fidelite d'incorporation de monomeres
IL161357A0 (en) * 2001-10-26 2004-09-27 Immunivest Corp Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample
CA2472029C (fr) 2001-11-26 2014-04-15 Keck Graduate Institute Procede, appareil et article de regulation microfluidique par electromouillage destines a des analyses chimiques, biochimiques, biologiques et analogues
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
WO2004018497A2 (fr) 2002-08-23 2004-03-04 Solexa Limited Nucleotides modifies
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
AU2004243690A1 (en) * 2003-03-03 2004-12-09 Burstein Technologies, Inc. Methods and apparatus for use in detection and quantitation of various cell types and use of optical bio-disc for performing same
TW200510723A (en) 2003-07-15 2005-03-16 Bioarray Solutions Ltd Concurrent optimization in selection of primer and capture probe sets for nucleic acid analysis
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
CA2543350C (fr) 2003-10-24 2011-04-26 Adhesives Research, Inc. Films disintegrables destines a des dispositifs de diagnostic
DE602004021624D1 (de) 2003-11-17 2009-07-30 Koninkl Philips Electronics Nv System zur handhabung einer fluidmenge
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
CN101914620B (zh) 2004-09-17 2014-02-12 加利福尼亚太平洋生命科学公司 核酸测序的方法
WO2006064199A1 (fr) 2004-12-13 2006-06-22 Solexa Limited Procede ameliore de detection de nucleotides
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
JP5897780B2 (ja) 2005-01-28 2016-03-30 デューク ユニバーシティ プリント回路基板上の液滴操作装置及び方法
EP1888743B1 (fr) 2005-05-10 2011-08-03 Illumina Cambridge Limited Polymerases ameliorees
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
US7464936B2 (en) 2005-07-19 2008-12-16 New Vision Gaming & Development, Inc. Method of playing a video poker game
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP2018622B1 (fr) 2006-03-31 2018-04-25 Illumina, Inc. Systèmes pour analyse de séquençage par synthèse
CA2680061C (fr) 2006-04-18 2015-10-13 Duke University Biochimie fondee sur les gouttelettes
JP4881950B2 (ja) 2006-07-10 2012-02-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス
US7414163B1 (en) 2006-07-28 2008-08-19 Uop Llc Iridium and germanium-containing catalysts and alkylaromatic transalkylation processes using such catalysts
WO2008051530A2 (fr) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Enzymes polymèrases et réactifs pour le séquençage amélioré d'acides nucléiques
US9266076B2 (en) 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
US8872527B2 (en) 2007-02-15 2014-10-28 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
WO2009003184A1 (fr) 2007-06-27 2008-12-31 Digital Biosystems Appareil microfluidique numerique destine a des processus chimiques d'echange de chaleur
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2359289A4 (fr) 2008-11-26 2015-11-11 Illumina Inc Procédés et systèmes d'analyse de données de séquençage
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
KR101423936B1 (ko) 2009-03-11 2014-07-29 (주)바이오니아 실시간 핵산 분석 통합 장치 및 이를 이용한 타겟 핵산의 검출방법
WO2011002957A2 (fr) 2009-07-01 2011-01-06 Advanced Liquid Logic, Inc. Dispositifs actionneurs de gouttelettes et procédés
WO2011038403A1 (fr) 2009-09-28 2011-03-31 Yuling Luo Procédés de détection de séquences d'acide nucléique à spécificité élevée
EP2539450B1 (fr) 2010-02-25 2016-02-17 Advanced Liquid Logic, Inc. Procédé de fabrication de banques d'acide nucléique
US9902950B2 (en) * 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
EP2635679B1 (fr) * 2010-11-05 2017-04-19 Illumina, Inc. Liaison entre des lectures de séquences à l'aide de codes marqueurs appariés
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
ES2687761T3 (es) * 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Métodos de identificación de múltiples epítopos en células
JP6017458B2 (ja) * 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
WO2012108864A1 (fr) 2011-02-08 2012-08-16 Illumina, Inc. Enrichissement sélectif d'acides nucléiques
EP3736281A1 (fr) * 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions et méthodes de marquage moléculaire
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
EP2718465B1 (fr) 2011-06-09 2022-04-13 Illumina, Inc. Procede de fabrication d'un reseau d'analytes
EP3305400A3 (fr) 2011-10-28 2018-06-06 Illumina, Inc. Système et procédé de fabrication de microréseau
WO2013078470A2 (fr) * 2011-11-22 2013-05-30 MOTIF, Active Isolation multiplex d'acides nucléiques associés à des protéines
CN102703426A (zh) * 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
WO2014028378A2 (fr) 2012-08-13 2014-02-20 The Regents Of The University Of California Procédés et systèmes de détection de composants biologiques
KR102190198B1 (ko) * 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
CN104032377A (zh) * 2014-06-30 2014-09-10 北京诺禾致源生物信息科技有限公司 单细胞转录组测序文库的构建方法及其应用

Also Published As

Publication number Publication date
RU2761432C2 (ru) 2021-12-08
RU2717491C2 (ru) 2020-03-23
CN116083546A (zh) 2023-05-09
EP3256604A2 (fr) 2017-12-20
WO2016130704A3 (fr) 2016-10-06
EP3256604B1 (fr) 2020-03-25
ES2786652T3 (es) 2020-10-13
AU2016219328A1 (en) 2017-08-17
US20180273933A1 (en) 2018-09-27
RU2017127628A3 (fr) 2019-03-12
JP2018509140A (ja) 2018-04-05
CN117106871A (zh) 2023-11-24
JP7001475B2 (ja) 2022-01-19
RU2020110764A3 (fr) 2021-04-21
CN107406890B (zh) 2023-07-18
EP3725893A1 (fr) 2020-10-21
BR112017017025A2 (pt) 2018-04-10
RU2017127628A (ru) 2019-03-12
BR112017017025B1 (pt) 2024-01-02
KR20170135834A (ko) 2017-12-08
DK3256604T3 (da) 2020-05-25
CA2975739C (fr) 2022-12-06
PT3256604T (pt) 2020-05-18
AU2016219328B2 (en) 2022-04-21
KR20200020997A (ko) 2020-02-26
SG11201706504RA (en) 2017-09-28
WO2016130704A2 (fr) 2016-08-18
CN107406890A (zh) 2017-11-28
CA2975739A1 (fr) 2016-08-18
RU2020110764A (ru) 2020-05-29
US11634707B2 (en) 2023-04-25
US20230374493A1 (en) 2023-11-23
AU2022202345A1 (en) 2022-04-28
CA3174951A1 (fr) 2016-08-18
KR20210135626A (ko) 2021-11-15

Similar Documents

Publication Publication Date Title
SG11201706504RA (en) Methods and compositions for analyzing cellular components
HK1257295A1 (zh) 用於免疫腫瘤學的組合物和方法
GB2546350B (en) Compositions and methods
IL248235B (en) Method and compositions for cellular immunotherapy
HK1253306A1 (zh) 抗ntb-a抗體及相關組合物和方法
IL253341B (en) Methods and preparations for combined immunotherapy
HK1248590A1 (zh) 用於治療貧血的組合物和方法
GB201718876D0 (en) Antithrombin-heparin compositions and methods
IL268684A (en) Preparations and methods for immuno-oncology
IL258292A (en) Methods and compositions for reducing metastases
HK1245158A1 (zh) 阿吡莫德組合物及其使用方法
IL246879A0 (en) Preparations of Epilimod and methods of using them
HK1254984A1 (zh) 用於細胞重編程的方法和組合物
GB201518379D0 (en) Analytical method and composition
IL274524A (en) Preparations and methods for aquaculture
GB201520255D0 (en) Composition and method
GB201522144D0 (en) Compositions and uses and methods relating thereto
GB201512996D0 (en) Compositions and methods
GB201509420D0 (en) Composition and method
GB201514413D0 (en) Compositions and methods
GB201513492D0 (en) Method and composition
IL256261B (en) Hide1 compositions and methods
GB201522554D0 (en) Methods and compositions
GB201518167D0 (en) Methods and compositions
GB201514415D0 (en) Compositions and methods