SG11201706504RA - Methods and compositions for analyzing cellular components - Google Patents
Methods and compositions for analyzing cellular componentsInfo
- Publication number
- SG11201706504RA SG11201706504RA SG11201706504RA SG11201706504RA SG11201706504RA SG 11201706504R A SG11201706504R A SG 11201706504RA SG 11201706504R A SG11201706504R A SG 11201706504RA SG 11201706504R A SG11201706504R A SG 11201706504RA SG 11201706504R A SG11201706504R A SG 11201706504RA
- Authority
- SG
- Singapore
- Prior art keywords
- compositions
- methods
- cellular components
- analyzing cellular
- analyzing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562114505P | 2015-02-10 | 2015-02-10 | |
PCT/US2016/017391 WO2016130704A2 (en) | 2015-02-10 | 2016-02-10 | Methods and compositions for analyzing cellular components |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201706504RA true SG11201706504RA (en) | 2017-09-28 |
Family
ID=55587329
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201706504RA SG11201706504RA (en) | 2015-02-10 | 2016-02-10 | Methods and compositions for analyzing cellular components |
SG10202104816QA SG10202104816QA (en) | 2015-02-10 | 2016-02-10 | Methods and compositions for analyzing cellular components |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10202104816QA SG10202104816QA (en) | 2015-02-10 | 2016-02-10 | Methods and compositions for analyzing cellular components |
Country Status (14)
Country | Link |
---|---|
US (2) | US11634707B2 (en) |
EP (2) | EP3725893A1 (en) |
JP (1) | JP7001475B2 (en) |
KR (4) | KR20200020997A (en) |
CN (3) | CN116083546A (en) |
AU (2) | AU2016219328B2 (en) |
BR (1) | BR112017017025B1 (en) |
CA (2) | CA2975739C (en) |
DK (1) | DK3256604T3 (en) |
ES (1) | ES2786652T3 (en) |
PT (1) | PT3256604T (en) |
RU (2) | RU2761432C2 (en) |
SG (2) | SG11201706504RA (en) |
WO (1) | WO2016130704A2 (en) |
Families Citing this family (120)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9476095B2 (en) | 2011-04-15 | 2016-10-25 | The Johns Hopkins University | Safe sequencing system |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3447495B2 (en) | 2012-10-29 | 2024-03-13 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
WO2014124338A1 (en) | 2013-02-08 | 2014-08-14 | 10X Technologies, Inc. | Polynucleotide barcode generation |
EP3102722B1 (en) | 2014-02-04 | 2020-08-26 | Jumpcode Genomics, Inc. | Genome fractioning |
WO2015157567A1 (en) | 2014-04-10 | 2015-10-15 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
MX2016016902A (en) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Methods of analyzing nucleic acids from individual cells or cell populations. |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
CN107407638B (en) | 2014-12-03 | 2021-01-12 | 伊索普莱西斯公司 | Analysis and screening of cellular secretion characteristics |
CN107427808B (en) | 2015-01-12 | 2020-10-23 | 10X基因组学有限公司 | Method and system for preparing nucleic acid sequencing library and library prepared by using same |
US10697000B2 (en) | 2015-02-24 | 2020-06-30 | 10X Genomics, Inc. | Partition processing methods and systems |
US10968536B2 (en) * | 2015-02-25 | 2021-04-06 | Jumpcode Genomics, Inc. | Methods and compositions for sequencing |
US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
US11339427B2 (en) | 2016-02-12 | 2022-05-24 | Jumpcode Genomics, Inc. | Method for target specific RNA transcription of DNA sequences |
KR102379048B1 (en) | 2016-05-02 | 2022-03-28 | 엔코디아, 인코포레이티드 | Macromolecular Analysis Using Encoding Nucleic Acids |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US10894990B2 (en) | 2016-05-17 | 2021-01-19 | Shoreline Biome, Llc | High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures |
WO2017214557A1 (en) | 2016-06-10 | 2017-12-14 | Counsyl, Inc. | Nucleic acid sequencing adapters and uses thereof |
US11535883B2 (en) | 2016-07-22 | 2022-12-27 | Illumina, Inc. | Single cell whole genome libraries and combinatorial indexing methods of making thereof |
WO2018057779A1 (en) * | 2016-09-23 | 2018-03-29 | Jianbiao Zheng | Compositions of synthetic transposons and methods of use thereof |
US11854666B2 (en) | 2016-09-29 | 2023-12-26 | Myriad Women's Health, Inc. | Noninvasive prenatal screening using dynamic iterative depth optimization |
DK3538891T3 (en) | 2016-11-11 | 2022-03-28 | Isoplexis Corp | COMPOSITIONS AND PROCEDURES FOR CONTEMPORARY GENOMIC, TRANSCRIPTOMIC AND PROTEOMIC ANALYSIS OF SINGLE CELLS |
CN110226084A (en) | 2016-11-22 | 2019-09-10 | 伊索普莱克西斯公司 | For the systems, devices and methods of cell capture and its manufacturing method |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
KR102551666B1 (en) * | 2016-12-29 | 2023-07-04 | 일루미나, 인코포레이티드 | An assay system for orthogonal access to and tagging of biomolecules within cell compartments |
WO2018140695A1 (en) * | 2017-01-27 | 2018-08-02 | Integrated Dna Technologies, Inc. | Construction of next generation sequencing (ngs) libraries using competitive strand displacement |
US10894979B2 (en) | 2017-01-27 | 2021-01-19 | Integrated Dna Technologies, Inc. | Construction of next generation sequencing (NGS) libraries using competitive strand displacement |
CN117512066A (en) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | Method and system for droplet-based single cell bar coding |
US10752946B2 (en) | 2017-01-31 | 2020-08-25 | Myriad Women's Health, Inc. | Methods and compositions for enrichment of target polynucleotides |
WO2018144216A1 (en) * | 2017-01-31 | 2018-08-09 | Counsyl, Inc. | Methods and compositions for enrichment of target polynucleotides |
US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
IL263118B2 (en) * | 2017-02-21 | 2023-12-01 | Illumina Inc | Tagmentation using immobilized transposomes with linkers |
WO2018175907A1 (en) | 2017-03-24 | 2018-09-27 | Counsyl, Inc. | Copy number variant caller |
FI3842545T3 (en) | 2017-04-23 | 2023-01-31 | Compositions and methods for improving sample identification in indexed nucleic acid libraries | |
CA3059839C (en) | 2017-04-23 | 2023-01-03 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
EP3872187B1 (en) | 2017-04-23 | 2022-09-21 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
US10975430B2 (en) | 2017-04-23 | 2021-04-13 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
CN110799653A (en) | 2017-05-01 | 2020-02-14 | 伊鲁米那股份有限公司 | Optimal index sequences for multiple massively parallel sequencing |
AU2018262331A1 (en) * | 2017-05-05 | 2019-11-21 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
US11028436B2 (en) | 2017-05-08 | 2021-06-08 | Illumina, Inc. | Universal short adapters for indexing of polynucleotide samples |
JP2020522243A (en) | 2017-05-23 | 2020-07-30 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Multiplexed end-tagging amplification of nucleic acids |
CN109526228B (en) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | Single cell analysis of transposase accessible chromatin |
US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
ES2959047T3 (en) | 2017-06-07 | 2024-02-19 | Univ Oregon Health & Science | Single Cell Whole Genome Libraries for Methylation Sequencing |
CN110785813A (en) | 2017-07-31 | 2020-02-11 | 伊鲁米那股份有限公司 | Sequencing system with multi-path biological sample aggregation |
EP4289967A3 (en) | 2017-08-01 | 2024-03-20 | Illumina, Inc. | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells |
EP3665308A1 (en) | 2017-08-07 | 2020-06-17 | The Johns Hopkins University | Methods and materials for assessing and treating cancer |
JP7241069B2 (en) | 2017-09-25 | 2023-03-16 | フレッド ハッチンソン キャンサー センター | Highly efficient targeted in situ genome-wide profiling |
US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
MX2020004559A (en) | 2017-10-31 | 2020-10-05 | Encodia Inc | Kits for analysis using nucleic acid encoding and/or label. |
WO2019099751A1 (en) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
RU2755738C2 (en) * | 2018-01-08 | 2021-09-20 | Иллюмина, Инк. | Systems and apparatuses for sequencing with high throughput capacity with detection based on semiconductors |
EP3752832A1 (en) | 2018-02-12 | 2020-12-23 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
CA3067140C (en) | 2018-02-13 | 2023-03-21 | Illumina, Inc. | Dna sequencing using hydrogel beads |
US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
CA3064622A1 (en) | 2018-04-02 | 2019-10-10 | Illumina, Inc. | Compositions and methods for making controls for sequence-based genetic testing |
EP3775271A1 (en) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
KR20240052875A (en) * | 2018-04-20 | 2024-04-23 | 일루미나, 인코포레이티드 | Methods of encapsulating single cells, the encapsulated cells and uses thereof |
US11142787B2 (en) * | 2018-05-07 | 2021-10-12 | Bitbiome, Inc. | Method for performing single-cell analysis and device therefor |
CA3067435C (en) * | 2018-05-17 | 2023-09-12 | Illumina, Inc. | High-throughput single-cell sequencing with reduced amplification bias |
SG11202000905PA (en) * | 2018-06-04 | 2020-02-27 | Illumina Inc | High-throughput single-cell transcriptome libraries and methods of making and of using |
US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
AU2019364545A1 (en) * | 2018-10-26 | 2021-04-29 | Illumina, Inc. | Modulating polymer beads for DNA processing |
CN109540855B (en) * | 2018-11-07 | 2021-03-23 | 沈阳化工大学 | Preparation method of fluorescent gold-shell magnetic ellipsoid |
EP3878936A4 (en) * | 2018-11-07 | 2022-08-10 | The University of Tokyo | Method for detecting genome-related information of cell coexisting with one or more substances to be detected |
EP4293126A3 (en) * | 2018-11-30 | 2024-01-17 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
WO2020114918A1 (en) | 2018-12-05 | 2020-06-11 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
AU2019411272A1 (en) | 2018-12-18 | 2021-01-07 | Illumina Cambridge Limited | Methods and compositions for paired end sequencing using a single surface primer |
FI3899037T3 (en) | 2018-12-19 | 2023-11-21 | Illumina Inc | Methods for improving polynucleotide cluster clonality priority |
US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
WO2020146603A1 (en) * | 2019-01-09 | 2020-07-16 | Qiagen Sciences, Llc | Methods of detecting analytes and compositions thereof |
TW202045709A (en) | 2019-01-29 | 2020-12-16 | 美商伊路米納有限公司 | Flow cells |
TW202100247A (en) | 2019-01-29 | 2021-01-01 | 美商伊路米納有限公司 | Flow cells |
TW202043486A (en) | 2019-01-29 | 2020-12-01 | 美商伊路米納有限公司 | Sequencing kits |
WO2020163174A1 (en) | 2019-02-04 | 2020-08-13 | Illumina, Inc. | Microfluidic droplet generators |
US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
KR20210134598A (en) | 2019-03-01 | 2021-11-10 | 일루미나, 인코포레이티드 | High-throughput single-nuclear and single-cell libraries and methods of making and using same |
EP3963087A1 (en) | 2019-04-29 | 2022-03-09 | Illumina Inc | Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment |
CA3138367A1 (en) | 2019-04-30 | 2020-11-05 | Encodia, Inc. | Methods for preparing analytes and related kits |
EP3976827A4 (en) | 2019-05-31 | 2023-10-18 | Illumina, Inc. | Obtaining information from a biological sample in a flow cell |
CN111088331B (en) * | 2019-12-16 | 2023-04-14 | 中国科学院苏州生物医学工程技术研究所 | Unimolecular sequencing method based on piezoelectric acoustic wave sensor |
CA3134746A1 (en) | 2019-12-19 | 2021-06-24 | Illumina, Inc. | High-throughput single-cell libraries and methods of making and of using |
CA3177286A1 (en) | 2020-05-12 | 2021-11-18 | Illumina Inc. | Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase |
WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
WO2022006081A1 (en) | 2020-06-30 | 2022-01-06 | Illumina, Inc. | Catalytically controlled sequencing by synthesis to produce scarless dna |
CN116018412A (en) | 2020-07-08 | 2023-04-25 | Illumina公司 | Beads as transposome vectors |
EP4196600A1 (en) * | 2020-08-14 | 2023-06-21 | Factorial Diagnostics, Inc. | In situ library preparation for sequencing |
KR20230051508A (en) * | 2020-08-18 | 2023-04-18 | 일루미나, 인코포레이티드 | Sequence-specific targeted translocation and selection and sorting of nucleic acids |
CA3194081A1 (en) * | 2020-10-15 | 2022-04-21 | Massa SHOURA | Detection and analysis of structural variations in genomes |
KR102576409B1 (en) * | 2021-01-18 | 2023-09-08 | 충남대학교병원 | Method for preparing cell-section using alginate bead |
EP4298244A1 (en) | 2021-02-23 | 2024-01-03 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
KR102526386B1 (en) * | 2021-03-19 | 2023-05-02 | 충남대학교병원 | Method for cell block production using alginate-PVA composite beads |
WO2022272260A1 (en) | 2021-06-23 | 2022-12-29 | Illumina, Inc. | Compositions, methods, kits, cartridges, and systems for sequencing reagents |
US11753677B2 (en) | 2021-11-10 | 2023-09-12 | Encodia, Inc. | Methods for barcoding macromolecules in individual cells |
US20230193147A1 (en) | 2021-12-21 | 2023-06-22 | Illumina Cambridge Limited | Wax-microsphere matrix compositions and methods of making and using the same |
EP4206674A1 (en) | 2021-12-28 | 2023-07-05 | Encodia, Inc. | High-throughput serotyping and antibody profiling assays |
CA3222937A1 (en) | 2021-12-29 | 2023-07-06 | Jonathan Mark Boutell | Methods of nucleic acid sequencing using surface-bound primers |
CA3223722A1 (en) | 2022-04-07 | 2023-10-12 | Illumina, Inc. | Altered cytidine deaminases and methods of use |
WO2023209606A1 (en) | 2022-04-29 | 2023-11-02 | Illumina Cambridge Limited | Methods and systems for encapsulating lyophilised microspheres |
EP4272764A1 (en) | 2022-05-03 | 2023-11-08 | Scipio Bioscience | Method of complexing biological units with particles |
GB202208728D0 (en) * | 2022-06-14 | 2022-07-27 | Cambridge Entpr Ltd | Methods for spatial genomic, epigenomic and multi-omic profiling using transposases and light-activated spatial barcoding |
WO2023245129A2 (en) * | 2022-06-15 | 2023-12-21 | Quantum-Si Incorporated | Directed protein evolution |
WO2024073043A1 (en) | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Methods of using cpg binding proteins in mapping modified cytosine nucleotides |
WO2024069581A1 (en) | 2022-09-30 | 2024-04-04 | Illumina Singapore Pte. Ltd. | Helicase-cytidine deaminase complexes and methods of use |
WO2024073047A1 (en) | 2022-09-30 | 2024-04-04 | Illumina, Inc. | Cytidine deaminases and methods of use in mapping modified cytosine nucleotides |
WO2024118903A1 (en) | 2022-11-30 | 2024-06-06 | Illumina, Inc. | Chemoenzymatic correction of false positive uracil transformations |
Family Cites Families (92)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
US5637684A (en) | 1994-02-23 | 1997-06-10 | Isis Pharmaceuticals, Inc. | Phosphoramidate and phosphorothioamidate oligomeric compounds |
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
ES2563643T3 (en) | 1997-04-01 | 2016-03-15 | Illumina Cambridge Limited | Nucleic acid sequencing method |
US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
CA2256128A1 (en) | 1998-12-29 | 2000-06-29 | Stephen William Davies | Coded dna processing |
US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
CA2415897A1 (en) | 2000-07-07 | 2002-01-17 | Susan H. Hardin | Real-time sequence determination |
WO2002007503A1 (en) | 2000-07-25 | 2002-01-31 | The Regents Of The University Of California | Electrowetting-driven micropumping |
US6773566B2 (en) | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
EP1354064A2 (en) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
WO2003035894A2 (en) * | 2001-10-26 | 2003-05-01 | Immunivest Corporation | Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample |
WO2003045556A2 (en) | 2001-11-26 | 2003-06-05 | Keck Graduate Institute | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
FR2841063B1 (en) | 2002-06-18 | 2004-09-17 | Commissariat Energie Atomique | DEVICE FOR DISPLACING SMALL VOLUMES OF LIQUID ALONG A MICRO-CATENARY BY ELECTROSTATIC FORCES |
DK3363809T3 (en) | 2002-08-23 | 2020-05-04 | Illumina Cambridge Ltd | MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE |
US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
US7547380B2 (en) | 2003-01-13 | 2009-06-16 | North Carolina State University | Droplet transportation devices and methods having a fluid surface |
CN101044404A (en) | 2003-03-03 | 2007-09-26 | 长冈实业株式会社 | Methods and apparatus for use in detection and quantitation of various cell types and use of optical bio-disc for performing same |
WO2005010200A2 (en) | 2003-07-15 | 2005-02-03 | Bioarray Solutions, Ltd. | Concurrent optimization in selection of primer and capture probe sets for nucleic acid analysis |
GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
DK1695094T3 (en) | 2003-10-24 | 2013-09-16 | Adhesives Res Inc | Disintegrating films for diagnostic devices |
JP4773360B2 (en) | 2003-11-17 | 2011-09-14 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | System for manipulating fluids |
EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
FR2866493B1 (en) | 2004-02-16 | 2010-08-20 | Commissariat Energie Atomique | DEVICE FOR CONTROLLING THE DISPLACEMENT OF A DROP BETWEEN TWO OR MORE SOLID SUBSTRATES |
FR2872715B1 (en) | 2004-07-08 | 2006-11-17 | Commissariat Energie Atomique | MICROREACTOR DROP |
FR2872809B1 (en) | 2004-07-09 | 2006-09-15 | Commissariat Energie Atomique | METHOD OF ADDRESSING ELECTRODES |
JP2006058031A (en) | 2004-08-17 | 2006-03-02 | Hitachi High-Technologies Corp | Chemical analyzer |
CN101914620B (en) | 2004-09-17 | 2014-02-12 | 加利福尼亚太平洋生命科学公司 | Method for analysis of molecules |
EP1828412B2 (en) | 2004-12-13 | 2019-01-09 | Illumina Cambridge Limited | Improved method of nucleotide detection |
US7458661B2 (en) | 2005-01-25 | 2008-12-02 | The Regents Of The University Of California | Method and apparatus for promoting the complete transfer of liquid drops from a nozzle |
PL1859330T3 (en) | 2005-01-28 | 2013-01-31 | Univ Duke | Apparatuses and methods for manipulating droplets on a printed circuit board |
JP4990886B2 (en) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | Improved polymerase |
US20090081688A1 (en) | 2005-06-20 | 2009-03-26 | Advanced Cell Diagnostics | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
US7464936B2 (en) | 2005-07-19 | 2008-12-16 | New Vision Gaming & Development, Inc. | Method of playing a video poker game |
GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
US20070023292A1 (en) | 2005-07-26 | 2007-02-01 | The Regents Of The University Of California | Small object moving on printed circuit board |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
US8241573B2 (en) | 2006-03-31 | 2012-08-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
EP2016091B1 (en) | 2006-04-18 | 2010-12-08 | Advanced Liquid Logic, Inc. | Droplet-based biochemistry |
EP2040082A4 (en) | 2006-07-10 | 2014-04-23 | Hitachi High Tech Corp | Liquid transfer device |
US7414163B1 (en) | 2006-07-28 | 2008-08-19 | Uop Llc | Iridium and germanium-containing catalysts and alkylaromatic transalkylation processes using such catalysts |
WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
WO2008055256A2 (en) | 2006-11-02 | 2008-05-08 | The Regents Of The University Of California | Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip |
US8872527B2 (en) | 2007-02-15 | 2014-10-28 | Advanced Liquid Logic, Inc. | Capacitance detection in a droplet actuator |
US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
WO2008134153A1 (en) | 2007-04-23 | 2008-11-06 | Advanced Liquid Logic, Inc. | Bead-based multiplexed analytical methods and instrumentation |
WO2009003184A1 (en) | 2007-06-27 | 2008-12-31 | Digital Biosystems | Digital microfluidics based apparatus for heat-exchanging chemical processes |
US8093064B2 (en) | 2008-05-15 | 2012-01-10 | The Regents Of The University Of California | Method for using magnetic particles in droplet microfluidics |
US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
EP2359289A4 (en) | 2008-11-26 | 2015-11-11 | Illumina Inc | Methods and systems for analysis of sequencing data |
US20100323348A1 (en) | 2009-01-31 | 2010-12-23 | The Regents Of The University Of Colorado, A Body Corporate | Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process |
KR101423936B1 (en) | 2009-03-11 | 2014-07-29 | (주)바이오니아 | Universal automatic apparatus for real time monitoring of products of nucleic acid amplification reaction and method thereof |
WO2011002957A2 (en) | 2009-07-01 | 2011-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
US20130023433A1 (en) | 2009-09-28 | 2013-01-24 | Yuling Luo | Methods of detecting nucleic acid sequences with high specificity |
ES2565563T3 (en) | 2010-02-25 | 2016-04-05 | Advanced Liquid Logic, Inc. | Method for preparing nucleic acid libraries |
GB2497912B (en) * | 2010-10-08 | 2014-06-04 | Harvard College | High-throughput single cell barcoding |
WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
US9074251B2 (en) * | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
US10144950B2 (en) * | 2011-01-31 | 2018-12-04 | Roche Sequencing Solutions, Inc. | Methods of identifying multiple epitopes in cells |
CA2826131C (en) * | 2011-02-02 | 2019-11-05 | Jay Ashok Shendure | Massively parallel continguity mapping |
WO2012108864A1 (en) | 2011-02-08 | 2012-08-16 | Illumina, Inc. | Selective enrichment of nucleic acids |
US9150852B2 (en) * | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
CN103890245B (en) * | 2011-05-20 | 2020-11-17 | 富鲁达公司 | Nucleic acid encoding reactions |
GB201108678D0 (en) | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
WO2012170936A2 (en) | 2011-06-09 | 2012-12-13 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
CA3003082C (en) | 2011-10-28 | 2020-12-15 | Illumina, Inc. | Microarray fabrication system and method |
WO2013078470A2 (en) * | 2011-11-22 | 2013-05-30 | MOTIF, Active | Multiplex isolation of protein-associated nucleic acids |
CN102703426A (en) * | 2012-05-21 | 2012-10-03 | 吴江汇杰生物科技有限公司 | Method for constructing a nucleic acid library, reagent and kit |
US9977861B2 (en) | 2012-07-18 | 2018-05-22 | Illumina Cambridge Limited | Methods and systems for determining haplotypes and phasing of haplotypes |
GB2519906B (en) * | 2012-08-13 | 2017-02-08 | Univ California | Methods for detecting target nucleic acids in sample lysate droplets |
WO2014124338A1 (en) * | 2013-02-08 | 2014-08-14 | 10X Technologies, Inc. | Polynucleotide barcode generation |
ES2724824T3 (en) | 2013-03-13 | 2019-09-16 | Illumina Inc | Methods for nucleic acid sequencing |
CN104032377A (en) * | 2014-06-30 | 2014-09-10 | 北京诺禾致源生物信息科技有限公司 | Construction method of single cell transcriptome sequencing library and application of construction method |
-
2016
- 2016-02-10 EP EP20165203.9A patent/EP3725893A1/en active Pending
- 2016-02-10 KR KR1020207004910A patent/KR20200020997A/en not_active IP Right Cessation
- 2016-02-10 CN CN202310017511.3A patent/CN116083546A/en active Pending
- 2016-02-10 KR KR1020217035607A patent/KR20210135626A/en not_active Application Discontinuation
- 2016-02-10 CA CA2975739A patent/CA2975739C/en active Active
- 2016-02-10 WO PCT/US2016/017391 patent/WO2016130704A2/en active Application Filing
- 2016-02-10 EP EP16711039.4A patent/EP3256604B1/en active Active
- 2016-02-10 RU RU2020110764A patent/RU2761432C2/en active
- 2016-02-10 DK DK16711039.4T patent/DK3256604T3/en active
- 2016-02-10 CN CN201680017121.4A patent/CN107406890B/en active Active
- 2016-02-10 CA CA3174951A patent/CA3174951A1/en active Pending
- 2016-02-10 SG SG11201706504RA patent/SG11201706504RA/en unknown
- 2016-02-10 RU RU2017127628A patent/RU2717491C2/en active
- 2016-02-10 PT PT167110394T patent/PT3256604T/en unknown
- 2016-02-10 SG SG10202104816QA patent/SG10202104816QA/en unknown
- 2016-02-10 JP JP2017541101A patent/JP7001475B2/en active Active
- 2016-02-10 AU AU2016219328A patent/AU2016219328B2/en active Active
- 2016-02-10 US US15/549,334 patent/US11634707B2/en active Active
- 2016-02-10 CN CN202310862581.9A patent/CN117106871A/en active Pending
- 2016-02-10 KR KR1020247017690A patent/KR20240091073A/en unknown
- 2016-02-10 KR KR1020177025457A patent/KR20170135834A/en active Application Filing
- 2016-02-10 ES ES16711039T patent/ES2786652T3/en active Active
- 2016-02-10 BR BR112017017025-6A patent/BR112017017025B1/en active IP Right Grant
-
2022
- 2022-04-07 AU AU2022202345A patent/AU2022202345A1/en active Pending
-
2023
- 2023-02-27 US US18/114,509 patent/US20230374493A1/en active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
SG10202104816QA (en) | Methods and compositions for analyzing cellular components | |
HK1257295A1 (en) | Compositions and methods for immunooncology | |
HK1253306A1 (en) | Anti-ntb-a antibodies and related compositions and methods | |
GB2546350B (en) | Compositions and methods | |
IL248235A0 (en) | Method and compositions for cellular immunotherapy | |
IL253341A0 (en) | Methods and compositions for combination immunotherapy | |
HK1248590A1 (en) | Compositions and methods for treating anemia | |
GB201718876D0 (en) | Antithrombin-heparin compositions and methods | |
IL268684A (en) | Compositions and methods for immunooncology | |
IL258292A (en) | Methods and compositions for reducing metastases | |
HK1245158A1 (en) | Apilimod compositions and methods for using same | |
IL246879B (en) | Apilimod compositions and methods for using same | |
HK1254984A1 (en) | Methods and compositions for cellular reprogramming | |
GB201518379D0 (en) | Analytical method and composition | |
IL274524B2 (en) | Compositions and methods for aquaculturing | |
GB201520255D0 (en) | Composition and method | |
GB201512996D0 (en) | Compositions and methods | |
GB201509420D0 (en) | Composition and method | |
GB201514413D0 (en) | Compositions and methods | |
GB201513492D0 (en) | Method and composition | |
IL256261B (en) | Hide1 compositions and methods | |
GB201522554D0 (en) | Methods and compositions | |
GB201518167D0 (en) | Methods and compositions | |
GB201514415D0 (en) | Compositions and methods |