SE451461B - Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli - Google Patents
Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coliInfo
- Publication number
- SE451461B SE451461B SE7806086A SE7806086A SE451461B SE 451461 B SE451461 B SE 451461B SE 7806086 A SE7806086 A SE 7806086A SE 7806086 A SE7806086 A SE 7806086A SE 451461 B SE451461 B SE 451461B
- Authority
- SE
- Sweden
- Prior art keywords
- plasmid
- procedure
- dna
- coli
- insulin
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 4
- 239000002773 nucleotide Substances 0.000 title abstract 3
- 102000004877 Insulin Human genes 0.000 title abstract 2
- 108090001061 Insulin Proteins 0.000 title abstract 2
- 229940125396 insulin Drugs 0.000 title abstract 2
- 239000013612 plasmid Substances 0.000 title 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 3
- 238000010804 cDNA synthesis Methods 0.000 abstract 3
- 239000002299 complementary DNA Substances 0.000 abstract 3
- 244000005700 microbiome Species 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Diabetes (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80134377A | 1977-05-27 | 1977-05-27 | |
| US80502377A | 1977-06-09 | 1977-06-09 | |
| US89770978A | 1978-04-19 | 1978-04-19 | |
| US05897710 US4363877B1 (en) | 1977-09-23 | 1978-04-19 | Recombinant dna transfer vectors |
| US05/898,887 US4264731A (en) | 1977-05-27 | 1978-04-21 | DNA Joining method |
| US89900278A | 1978-04-26 | 1978-04-26 | |
| US89900378A | 1978-04-26 | 1978-04-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE7806086L SE7806086L (sv) | 1978-11-28 |
| SE451461B true SE451461B (sv) | 1987-10-12 |
Family
ID=27569921
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806086A SE451461B (sv) | 1977-05-27 | 1978-05-26 | Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli |
| SE8305327A SE8305327L (sv) | 1977-05-27 | 1983-09-29 | Isolering av differentierade celler |
| SE8305328A SE8305328D0 (sv) | 1977-05-27 | 1983-09-29 | Isolering av icke nedbruten rna |
| SE8305329A SE455794B (sv) | 1977-05-27 | 1983-09-29 | Plasmid med nukleotidsekvens som kodar for aminosyrasekvensen i humant tillvexthormon och forfarande for framstellning derav |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8305327A SE8305327L (sv) | 1977-05-27 | 1983-09-29 | Isolering av differentierade celler |
| SE8305328A SE8305328D0 (sv) | 1977-05-27 | 1983-09-29 | Isolering av icke nedbruten rna |
| SE8305329A SE455794B (sv) | 1977-05-27 | 1983-09-29 | Plasmid med nukleotidsekvens som kodar for aminosyrasekvensen i humant tillvexthormon och forfarande for framstellning derav |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPH0659218B2 (de) |
| AR (1) | AR225404A1 (de) |
| AU (1) | AU521903B2 (de) |
| BE (1) | BE867424A (de) |
| BG (1) | BG40319A3 (de) |
| CH (1) | CH642680A5 (de) |
| DD (1) | DD145927A5 (de) |
| DE (2) | DE2858357C2 (de) |
| DK (2) | DK233278A (de) |
| FI (1) | FI64640C (de) |
| FR (1) | FR2392033B1 (de) |
| GB (1) | GB1565190A (de) |
| GR (1) | GR73552B (de) |
| IL (1) | IL54790A (de) |
| IT (1) | IT1094934B (de) |
| LU (1) | LU79714A1 (de) |
| NL (1) | NL7805591A (de) |
| NZ (1) | NZ187300A (de) |
| PL (1) | PL140200B1 (de) |
| PT (1) | PT68082B (de) |
| SE (4) | SE451461B (de) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989010937A1 (en) * | 1988-05-11 | 1989-11-16 | Novo Nordisk A/S | Insulin analogues |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
| FR2422717B1 (fr) * | 1977-11-08 | 1985-12-06 | Genentech Inc | Procede permettant l'expression microbienne de polypeptides et moyens appropries |
| FI792481A (fi) * | 1978-08-11 | 1980-02-12 | Univ California | Syntes av enkaroytiskt protein genom anvaendning av mikro-organismer |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| JPS55150883A (en) * | 1979-05-11 | 1980-11-25 | Nakano Vinegar Co Ltd | Method of storing "mozuku" |
| GR68404B (de) * | 1979-06-01 | 1981-12-29 | Univ California | |
| FR2458584A1 (fr) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | Vecteurs pour le transfert et l'expression de materiel genetique dans une cellule eucaryote et procede pour la production d'une proteine determinee dans des cellules eucaryotes |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| GR70279B (de) * | 1979-09-12 | 1982-09-03 | Univ California | |
| IL58765A (en) * | 1979-11-21 | 1986-09-30 | Yeda Res & Dev | Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta |
| DE3001928A1 (de) * | 1980-01-19 | 1981-08-06 | Hoechst Ag, 6000 Frankfurt | Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| US4350764A (en) * | 1980-03-10 | 1982-09-21 | The Regents Of The University Of California | Microbiological synthesis of beta endorphin |
| CA1202581A (en) * | 1980-03-27 | 1986-04-01 | Saran A. Narang | Adaptor molecules for dna and their application to synthesis of gene-derived products |
| US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| IE52417B1 (en) * | 1980-12-12 | 1987-10-28 | Unilever Plc | Dna sequences encoding various allelic forms of mature thaumatin,recombinant plasmids comprising said dnas and a process for their preparation,bacterial cultures comprising said recombinant plasmids,and method for producing mature thaumatin |
| BR8108073A (pt) * | 1980-12-12 | 1982-09-21 | Unilever Nv | Sequencias de dna;plasmidios recombinantes;cultura bacteriana;e processo para a producao de preprotaumatina,pretaumatina ou protaumatina |
| US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
| US4748233A (en) * | 1982-03-23 | 1988-05-31 | Bristol-Myers Company | Alpha-interferon Gx-1 |
| CA1224432A (en) * | 1982-08-17 | 1987-07-21 | Gary N. Buell | Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield |
| US5411951A (en) * | 1984-10-04 | 1995-05-02 | Monsanto Company | Prolonged release of biologically active somatotropin |
| US5474980A (en) * | 1984-10-04 | 1995-12-12 | Monsanto Company | Prolonged release of biologically active somatotropins |
| DE4034702A1 (de) * | 1990-10-31 | 1992-05-07 | Siemens Ag | Verfahren und vorrichtung zur pruefung der aktiven werkzeuge von gesenkbiegemaschinen, insbesondere von biegestempeln |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
-
1978
- 1978-05-17 NZ NZ187300A patent/NZ187300A/xx unknown
- 1978-05-23 AR AR272286A patent/AR225404A1/es active
- 1978-05-23 GR GR56309A patent/GR73552B/el unknown
- 1978-05-23 NL NL7805591A patent/NL7805591A/ not_active Application Discontinuation
- 1978-05-24 BE BE187995A patent/BE867424A/xx not_active IP Right Cessation
- 1978-05-24 DE DE2858357A patent/DE2858357C2/de not_active Expired - Lifetime
- 1978-05-24 DE DE19782822568 patent/DE2822568A1/de active Granted
- 1978-05-24 PT PT68082A patent/PT68082B/pt unknown
- 1978-05-25 AU AU36488/78A patent/AU521903B2/en not_active Expired
- 1978-05-25 DD DD78205588A patent/DD145927A5/de unknown
- 1978-05-25 FR FR7815596A patent/FR2392033B1/fr not_active Expired
- 1978-05-25 GB GB22417/78A patent/GB1565190A/en not_active Expired
- 1978-05-26 FI FI781675A patent/FI64640C/fi not_active IP Right Cessation
- 1978-05-26 CH CH581078A patent/CH642680A5/de not_active IP Right Cessation
- 1978-05-26 DK DK782332A patent/DK233278A/da not_active Application Discontinuation
- 1978-05-26 SE SE7806086A patent/SE451461B/xx unknown
- 1978-05-26 IL IL54790A patent/IL54790A/xx unknown
- 1978-05-26 LU LU79714A patent/LU79714A1/xx unknown
- 1978-05-27 PL PL1978207176A patent/PL140200B1/pl unknown
- 1978-05-27 BG BG7839878A patent/BG40319A3/xx unknown
- 1978-05-27 JP JP53063756A patent/JPH0659218B2/ja not_active Expired - Lifetime
- 1978-05-29 IT IT23930/78A patent/IT1094934B/it active
-
1983
- 1983-09-29 SE SE8305327A patent/SE8305327L/sv not_active Application Discontinuation
- 1983-09-29 SE SE8305328A patent/SE8305328D0/xx not_active Application Discontinuation
- 1983-09-29 SE SE8305329A patent/SE455794B/sv not_active Application Discontinuation
-
1991
- 1991-01-04 DK DK000791A patent/DK791A/da not_active Application Discontinuation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989010937A1 (en) * | 1988-05-11 | 1989-11-16 | Novo Nordisk A/S | Insulin analogues |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SE451461B (sv) | Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli | |
| KR880014110A (ko) | 인체 프로아포지방단백질 a-i의 발현 | |
| Rossi et al. | An alternate method for synthesis of double-stranded DNA segments. | |
| WO2003008615A3 (en) | Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced succ or sucd gene | |
| DE3686217D1 (de) | Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen. | |
| EP0055945A3 (de) | Menschliches Proinsulin und dessen Analoge und Verfahren zur Herstellung durch mikrobielle polypeptide Expression und Umwandlung derselben in menschliches Insulin | |
| Silen et al. | Molecular analysis of the gene encoding α-lytic protease: evidence for a preproenzyme | |
| ES8205265A1 (es) | Un metodo para producir la hormona de crecimiento humano | |
| ES531855A0 (es) | Un metodo para producir eficazmente un factor de crecimiento similar a insulina humana | |
| NO173741C (no) | DNA-sekvens for modifisert protein C, vektor inneholdende denne, patte-dyrceller transfektert med vektorer, og fremgangsmaate for fremstilling av nevnte protein | |
| Beaudry et al. | An efficient strategy for the synthesis of circular RNA molecules. | |
| HUP9800329A2 (hu) | Nukleinsavkonstrukciók strukturális gének sejtciklus-függő módon szabályozott expresszálására | |
| Dube et al. | A structural requirement for codon-anticodon interaction on the ribosome | |
| CN110331147A (zh) | 一种mRNA的制备方法及其在肿瘤治疗中的应用 | |
| CN109456976B (zh) | 舞毒蛾JHAMT基因、其编码蛋白及其dsRNA在害虫防治中的应用 | |
| CN106047895B (zh) | 一种青蒿bZIP类转录因子编码序列及克隆方法与应用 | |
| CN104745584B (zh) | 一种用于干扰罗氏沼虾雄性生殖的核酸分子及其制备方法 | |
| JPS5452789A (en) | Preparation of sarcosine oxidase | |
| JPS5756495A (en) | Novel dna-introduction vector and recombinant dna | |
| CN114908083B (zh) | 一种双链环状dna体外快速合成的方法 | |
| Rauè et al. | Occurrence in Bacillus licheniformis of Two Species of 5‐S RNA with Multiple Differences in Primary Structure | |
| Inoue et al. | 5′‐Terminal Nucleotide Sequence of the Messenger RNA Coding for Bovine Corticotropin/β‐Lipotropin Precursor | |
| FI65447C (fi) | Foerfarande foer framstaellning av en bakterie som innehaolleren foer tillvaexthormon kodande nukleotidsekvens | |
| CN118028340A (zh) | 一种用于环状rna表达的核酸构建体及其在蛋白表达中的应用 | |
| CN115925862A (zh) | 一种鱼类神经肽nkb和nkbrp及其编码基因和应用 |