RU2741103C2 - Рекомбинантное получение стевиол-гликозидов - Google Patents

Рекомбинантное получение стевиол-гликозидов Download PDF

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RU2741103C2
RU2741103C2 RU2019135831A RU2019135831A RU2741103C2 RU 2741103 C2 RU2741103 C2 RU 2741103C2 RU 2019135831 A RU2019135831 A RU 2019135831A RU 2019135831 A RU2019135831 A RU 2019135831A RU 2741103 C2 RU2741103 C2 RU 2741103C2
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RU2019135831A (ru
RU2019135831A3 (fr
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Гохун МАО
Сяодань ЮЙ
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Конаджен Инк.
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/44Preparation of O-glycosides, e.g. glucosides
    • C12P19/56Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D13/00Finished or partly finished bakery products
    • A21D13/06Products with modified nutritive value, e.g. with modified starch content
    • A21D13/062Products with modified nutritive value, e.g. with modified starch content with modified sugar content; Sugar-free products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A23G3/34Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
    • A23G3/36Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
    • A23G3/38Sucrose-free products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23GCOCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G4/00Chewing gum
    • A23G4/06Chewing gum characterised by the composition containing organic or inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • A23L27/36Terpene glycosides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20Carbocyclic rings
    • C07H15/24Condensed ring systems having three or more rings
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • C12N9/1062Sucrose synthase (2.4.1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • CCHEMISTRY; METALLURGY
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    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)

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  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
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  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Inorganic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Seasonings (AREA)
RU2019135831A 2013-11-01 2014-10-03 Рекомбинантное получение стевиол-гликозидов RU2741103C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361898571P 2013-11-01 2013-11-01
US61/898,571 2013-11-01

Related Parent Applications (1)

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RU2016120635A Division RU2706789C2 (ru) 2013-11-01 2014-10-03 Рекомбинантное получение стевиол-гликозидов

Publications (3)

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RU2019135831A RU2019135831A (ru) 2020-01-20
RU2019135831A3 RU2019135831A3 (fr) 2020-06-19
RU2741103C2 true RU2741103C2 (ru) 2021-01-22

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RU2019135831A RU2741103C2 (ru) 2013-11-01 2014-10-03 Рекомбинантное получение стевиол-гликозидов
RU2016120635A RU2706789C2 (ru) 2013-11-01 2014-10-03 Рекомбинантное получение стевиол-гликозидов

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US (3) US20160251635A1 (fr)
EP (1) EP3063286B1 (fr)
JP (1) JP6530760B2 (fr)
CN (1) CN106103729B (fr)
AU (2) AU2014342939B2 (fr)
CA (1) CA2928940C (fr)
DK (1) DK3063286T3 (fr)
ES (1) ES2709439T3 (fr)
MX (1) MX367771B (fr)
RU (2) RU2741103C2 (fr)
WO (1) WO2015065650A2 (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2741103C2 (ru) 2013-11-01 2021-01-22 Конаджен Инк. Рекомбинантное получение стевиол-гликозидов
US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
ES2741741T3 (es) 2014-10-03 2020-02-12 Conagen Inc Edulcorantes no calóricos y métodos para sintetizar
CN107249356B (zh) 2014-12-17 2021-08-06 嘉吉公司 用于口服摄入或使用的甜菊醇糖苷化合物、组合物以及用于增强甜菊醇糖苷溶解度的方法
WO2016146711A1 (fr) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransférases
CN113683712B (zh) 2015-04-03 2022-10-21 帝斯曼知识产权资产管理有限公司 甜菊醇糖苷
WO2016168413A1 (fr) * 2015-04-14 2016-10-20 Conagen Inc. Production d'édulcorants non caloriques ayant recours à des catalyseurs de cellules entières modifiées
JP2018521636A (ja) * 2015-05-29 2018-08-09 カーギル・インコーポレイテッド 多段階供給を用いる、ステビオール配糖体を生成するための発酵方法
CN105348337B (zh) * 2015-11-19 2018-07-27 南京诺云生物科技有限公司 一种甜菊苷生物转化制备的甜菊糖衍生物、制备方法及其应用
CN105380224A (zh) * 2015-11-19 2016-03-09 南京诺云生物科技有限公司 一种新型甜菊糖衍生物、制备方法及其应用
US20200308616A1 (en) * 2015-11-30 2020-10-01 Purecircle Sdn Bhd Process for producing high purity steviol glycosides
WO2017198682A1 (fr) 2016-05-16 2017-11-23 Evolva Sa Production de glycosides de stéviol dans des hôtes de recombinaison
MX2018015534A (es) 2016-06-15 2019-03-14 Codexis Inc Beta-glucosidasas dise?adas y metodos de glucosilacion.
CN110191643B (zh) 2016-10-14 2023-06-06 科纳根公司 甜菊醇糖苷的生物合成生产及其工艺
JP6972123B2 (ja) 2016-10-21 2021-11-24 ペプシコ・インク 酵素法を使用するレバウディオサイドnを調製するための方法
AU2018215335B2 (en) 2017-02-03 2020-10-01 Tate & Lyle Solutions Usa Llc Engineered glycosyltransferases and steviol glycoside glucosylation methods
EP3592157A4 (fr) * 2017-03-06 2021-01-13 Conagen Inc. Production biosynthétique du glycoside de stéviol rébaudiosine d4 à partir de rébaudiosine e
CN111093390A (zh) * 2017-05-15 2020-05-01 谱赛科美国股份有限公司 高纯度甜菊醇糖苷
KR102792406B1 (ko) 2017-06-30 2025-04-08 코나겐 인크. 베타-글루코시다제에 의한 스테비올 글리코시드의 가수분해
CN109423486B (zh) * 2017-08-29 2022-02-25 中国科学院分子植物科学卓越创新中心 新型udp-糖基转移酶及其应用
US11365417B2 (en) 2017-09-12 2022-06-21 Bio Capital Holdings, LLC Biological devices and methods of use thereof to produce steviol glycosides
CN109868265B (zh) * 2017-12-01 2023-04-25 中国科学院分子植物科学卓越创新中心 新型糖基转移酶及其应用
BR112021001661A8 (pt) 2018-07-30 2022-08-09 Codexis Inc Glicosiltransferase modificada, polinucleotídeo modificado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase modificada, para produzir pelo menos uma variante de sacarose sintase, para glicosilação de um substrato, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d, composição, sacarose sintase modificada, pelo menos um rebaudiosídeo, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d.
WO2020097588A1 (fr) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthèse de mogrosides
JP7449586B2 (ja) * 2018-12-12 2024-03-14 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造
CN113388014B (zh) * 2020-03-12 2023-02-03 中科农福(北京)生物技术有限公司 Os02g0589400蛋白在调控植物化感作用中的应用
KR102628374B1 (ko) * 2021-10-19 2024-01-24 씨제이제일제당 주식회사 신규한 당전이효소 및 이의 용도
WO2023183832A1 (fr) 2022-03-23 2023-09-28 Sweegen, Inc. Édulcorant comprenant du rébaudioside m et de la brazzéine

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RU2427285C2 (ru) * 2007-03-14 2011-08-27 Дзе Консентрейт Мэньюфектуринг Компани Оф Аиэлэнд Продукты типа напитков, содержащие стевиолгликозиды и по меньшей мере одну кислоту
CN101701238B (zh) * 2009-10-14 2011-11-16 南京师范大学 一种由甜菊糖苷制备甜茶甙的方法
WO2013133689A1 (fr) * 2012-03-08 2013-09-12 Purecircle Sdn Bhd Rubusoside de haute pureté et procédé pour le produire
RU2599166C2 (ru) * 2011-12-19 2016-10-10 Дзе Кока-Кола Компани Способы очистки стевиоловых гликозидов и их применение

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US9631215B2 (en) * 2011-08-08 2017-04-25 Evolva Sa Recombinant production of steviol glycosides
RU2499427C1 (ru) * 2012-09-19 2013-11-27 Нина Васильевна Мелишева Композиция подсластителя
RU2741103C2 (ru) 2013-11-01 2021-01-22 Конаджен Инк. Рекомбинантное получение стевиол-гликозидов

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RU2427285C2 (ru) * 2007-03-14 2011-08-27 Дзе Консентрейт Мэньюфектуринг Компани Оф Аиэлэнд Продукты типа напитков, содержащие стевиолгликозиды и по меньшей мере одну кислоту
CN101701238B (zh) * 2009-10-14 2011-11-16 南京师范大学 一种由甜菊糖苷制备甜茶甙的方法
RU2599166C2 (ru) * 2011-12-19 2016-10-10 Дзе Кока-Кола Компани Способы очистки стевиоловых гликозидов и их применение
WO2013133689A1 (fr) * 2012-03-08 2013-09-12 Purecircle Sdn Bhd Rubusoside de haute pureté et procédé pour le produire

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CN106103729B (zh) 2020-07-07
MX2016005677A (es) 2016-10-28
EP3063286B1 (fr) 2018-12-05
JP6530760B2 (ja) 2019-06-12
RU2016120635A (ru) 2017-12-06
US10081826B2 (en) 2018-09-25
RU2016120635A3 (fr) 2018-05-31
US20170298404A1 (en) 2017-10-19
JP2017500056A (ja) 2017-01-05
RU2706789C2 (ru) 2019-11-21
DK3063286T3 (en) 2019-03-04
MX367771B (es) 2019-09-05
AU2017254856A1 (en) 2017-11-23
US20170081691A1 (en) 2017-03-23
EP3063286A4 (fr) 2017-06-21
RU2019135831A (ru) 2020-01-20
WO2015065650A2 (fr) 2015-05-07
US20160251635A1 (en) 2016-09-01
AU2014342939B2 (en) 2018-03-22
CN106103729A (zh) 2016-11-09
RU2019135831A3 (fr) 2020-06-19
EP3063286A2 (fr) 2016-09-07
CA2928940A1 (fr) 2015-05-07
ES2709439T3 (es) 2019-04-16
WO2015065650A3 (fr) 2015-11-05
CA2928940C (fr) 2021-12-14
US10253344B2 (en) 2019-04-09
AU2014342939A1 (en) 2016-05-19

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