RU2015126650A - Партнеры липокалина по слиянию - Google Patents

Партнеры липокалина по слиянию Download PDF

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RU2015126650A
RU2015126650A RU2015126650A RU2015126650A RU2015126650A RU 2015126650 A RU2015126650 A RU 2015126650A RU 2015126650 A RU2015126650 A RU 2015126650A RU 2015126650 A RU2015126650 A RU 2015126650A RU 2015126650 A RU2015126650 A RU 2015126650A
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protein
peptide
cleavage site
nottin
fusion protein
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RU2015126650A
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Джеймс ОЛСОН
Кристофер Мехлин
Колин КОРРЕНТИ
Роналд СТРОНГ
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Фред Хатчинсон Кэнсер Рисёрч Сентер
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1767—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43522—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from scorpions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99003—Heme oxygenase (1.14.99.3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

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  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
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  • Urology & Nephrology (AREA)
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Claims (23)

1. Способ получения слитого белка, причем способ включает экспрессию слитого белка в клетке, причем слитый белок представляет собой ноттин-подобный пептид и белок липокалин, в результате чего получают слитый белок.
2. Способ по п. 1, дополнительно включающий отделение ноттин-подобного пептида от белка липокалина, в результате чего получают ноттин-подобный пептид.
3. Способ по п. 2 отличающийся тем, что слитый белок дополнительно содержит сайт расщепления.
4. Способ по п. 3, отличающийся тем, что сайт расщепления представляет собой сайт расщепления для фурина, сайт расщепления для трипсина или сайт расщепления для протеазы TEV.
5. Способ по п. 3, отличающийся тем, что отделение ноттин-подобного пептида от белка липокалина происходит в результате расщепления в сайте расщепления в слитом белке.
6. Способ по п. 2, отличающийся тем, что отделение ноттин-подобного пептида от белка липокалина происходит после секреции слитого белка из клетки.
7. Способ по п. 6, отличающийся тем, что клетка представляет собой клетку млекопитающего.
8. Способ по п. 1, отличающийся тем, что ноттин-подобный пептид представляет собой PMP-D2, карбоксипептидазу картофеля, хувентоксин, императоксин, эпирегулин, мидкин, баббл-белок или конотоксин CVIC.
9. Способ по п. 1, отличающийся тем, что белок липокалин включает сидерокалин, Ex-FABP курицы или Q86 перепелки, и где слитый белок содержит следующую конструкцию: IgK SP-sFLAG-HIS-сидерокалин-TEV-пептид.
10. Композиция спитого белка, причем указанная композиция содержит:
ноттин-подобный пептид и
белок липокалин.
11. Композиция по п. 10, отличающаяся тем, что ноттин-подобный пептид выбран из группы, состоящей из PMP-D2, карбоксипептидазы картофеля, хувентоксина, императоксина, эпирегулина, мидкина, баббл-белка и конотоксина CIVC.
12. Композиция по п. 10, отличающаяся тем, что белок липокалин включает сидерокалин, Ex-FABP курицы или Q86 перепелки.
13. Композиция по п. 10, отличающаяся тем, что слитый белок дополнительно включает по меньшей мере одну последовательность из: начальной последовательности IgK, sFLAG, HIS и или TEV.
14. Композиция по п. 10, отличающаяся тем, что слитый белок получен путем прямого слияния каждой субъединицы со смежными субъединицами.
15. Композиция по п. 10, дополнительно содержащая линкерную последовательность между ноттин-подобным пептидом и белком липокалином.
16. Способ по п. 1, отличающийся тем, что белок липокаин включает сидерокалин и и с дополнительной нагрузкой сидерокалина лигандом.
17. Способ по п. 16, отличающийся тем, что лиганд содержит химиотерапевтический или радиоактивный агент.
18. Композиция по п. 10, дополнительно содержащая сайт расщепления между ноттин-подобным пептидом и белком липокаином.
19. Композиция по п. 18, в которой сайт расщепления представляет собой сайт расщепления для фурина, сайт расщепления для трипсина, сайт расщепления для протеазы TEV.
20. Композиция по п. 10, в которой белок липокаин содержит сидерокалин нагруженный лигандом.
21. Композиция по п. 20, в которой лиганд содержит химиотерапевтический или радиоактивный агент.
RU2015126650A 2012-12-10 2013-12-10 Партнеры липокалина по слиянию RU2015126650A (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261735516P 2012-12-10 2012-12-10
US61/735,516 2012-12-10
US201361794685P 2013-03-15 2013-03-15
US61/794,685 2013-03-15
PCT/US2013/074215 WO2014093403A1 (en) 2012-12-10 2013-12-10 Lipocalin fusion partners

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US (4) US20150316536A1 (ru)
EP (2) EP2928913A1 (ru)
JP (2) JP2016505251A (ru)
CN (2) CN105189540A (ru)
AU (2) AU2013359429A1 (ru)
BR (2) BR112015013525A2 (ru)
CA (2) CA2913127A1 (ru)
IL (2) IL239262A0 (ru)
RU (2) RU2015126650A (ru)
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WO (2) WO2014093403A1 (ru)

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