PT80797B - 54), (55) e (56) está colocado do lado oposto do eixo (A-A) relativamente ao tubo (53). 0 tubo central (53) dos tubos (54), (55) e (56) está disposto à distância (E) do eixo (A-A). Os tubos (54), (55) e (56) comunicam com um furo de carga (27’) e com um furo de descarga (31’). Os tubos (54), (55) θ (56) podem ser carregados com o produto (6) através do furo de carga (27’). 0 produto (6) pode ser retirado do tubo ou caixa (53) através do furo (31) e dos tubos (54) (55) e (5 através do furo (31’) sem desprender a tampa, da mesma maneira já descrita em ligação com as formas de realização anteriores. Os furos mutuamente opostos (52a) e (52b) são previstos na face terminal inferior do recipiente (52). Um de cada vez, os furos (52a) e (52b) podem cooperar com meios retentores - Google Patents
54), (55) e (56) está colocado do lado oposto do eixo (A-A) relativamente ao tubo (53). 0 tubo central (53) dos tubos (54), (55) e (56) está disposto à distância (E) do eixo (A-A). Os tubos (54), (55) e (56) comunicam com um furo de carga (27’) e com um furo de descarga (31’). Os tubos (54), (55) θ (56) podem ser carregados com o produto (6) através do furo de carga (27’). 0 produto (6) pode ser retirado do tubo ou caixa (53) através do furo (31) e dos tubos (54) (55) e (5 através do furo (31’) sem desprender a tampa, da mesma maneira já descrita em ligação com as formas de realização anteriores. Os furos mutuamente opostos (52a) e (52b) são previstos na face terminal inferior do recipiente (52). Um de cada vez, os furos (52a) e (52b) podem cooperar com meios retentores Download PDFInfo
- Publication number
- PT80797B PT80797B PT80797A PT8079785A PT80797B PT 80797 B PT80797 B PT 80797B PT 80797 A PT80797 A PT 80797A PT 8079785 A PT8079785 A PT 8079785A PT 80797 B PT80797 B PT 80797B
- Authority
- PT
- Portugal
- Prior art keywords
- specimens
- vessel
- reservoir
- treatment
- specimen
- Prior art date
Links
- 239000002826 coolant Substances 0.000 claims abstract 9
- 238000010438 heat treatment Methods 0.000 claims description 15
- 238000005538 encapsulation Methods 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 8
- 238000009413 insulation Methods 0.000 claims description 7
- 239000011229 interlayer Substances 0.000 claims description 6
- 238000006073 displacement reaction Methods 0.000 claims description 5
- 239000010410 layer Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000033228 biological regulation Effects 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 230000001276 controlling effect Effects 0.000 claims description 2
- 239000000110 cooling liquid Substances 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 239000000543 intermediate Substances 0.000 claims 2
- 238000000605 extraction Methods 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 34
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 73
- 239000000047 product Substances 0.000 description 31
- 229910052757 nitrogen Inorganic materials 0.000 description 30
- 238000011068 loading method Methods 0.000 description 12
- 229910052751 metal Inorganic materials 0.000 description 11
- 239000002184 metal Substances 0.000 description 11
- 230000018044 dehydration Effects 0.000 description 7
- 238000006297 dehydration reaction Methods 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000006116 polymerization reaction Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 239000003570 air Substances 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 229910001873 dinitrogen Inorganic materials 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920005830 Polyurethane Foam Polymers 0.000 description 1
- COQLPRJCUIATTQ-UHFFFAOYSA-N Uranyl acetate Chemical compound O.O.O=[U]=O.CC(O)=O.CC(O)=O COQLPRJCUIATTQ-UHFFFAOYSA-N 0.000 description 1
- VJMAITQRABEEKP-UHFFFAOYSA-N [6-(phenylmethoxymethyl)-1,4-dioxan-2-yl]methyl acetate Chemical compound O1C(COC(=O)C)COCC1COCC1=CC=CC=C1 VJMAITQRABEEKP-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000181 anti-adherent effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000011496 polyurethane foam Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
- F17C—VESSELS FOR CONTAINING OR STORING COMPRESSED, LIQUEFIED OR SOLIDIFIED GASES; FIXED-CAPACITY GAS-HOLDERS; FILLING VESSELS WITH, OR DISCHARGING FROM VESSELS, COMPRESSED, LIQUEFIED, OR SOLIDIFIED GASES
- F17C2270/00—Applications
- F17C2270/05—Applications for industrial use
- F17C2270/0509—"Dewar" vessels
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Combustion & Propulsion (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Treatment Of Sludge (AREA)
- Control Of Combustion (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19843425744 DE3425744A1 (de) | 1984-07-12 | 1984-07-12 | Vorrichtung zur entwaesserung und/oder polymerisationseinbettung biologischer proben bei tiefen temperaturen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PT80797A PT80797A (en) | 1985-08-01 |
| PT80797B true PT80797B (pt) | 1986-11-20 |
Family
ID=6240502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT80797A PT80797B (pt) | 1984-07-12 | 1985-07-11 | 54), (55) e (56) está colocado do lado oposto do eixo (A-A) relativamente ao tubo (53). 0 tubo central (53) dos tubos (54), (55) e (56) está disposto à distância (E) do eixo (A-A). Os tubos (54), (55) e (56) comunicam com um furo de carga (27’) e com um furo de descarga (31’). Os tubos (54), (55) θ (56) podem ser carregados com o produto (6) através do furo de carga (27’). 0 produto (6) pode ser retirado do tubo ou caixa (53) através do furo (31) e dos tubos (54) (55) e (5 através do furo (31’) sem desprender a tampa, da mesma maneira já descrita em ligação com as formas de realização anteriores. Os furos mutuamente opostos (52a) e (52b) são previstos na face terminal inferior do recipiente (52). Um de cada vez, os furos (52a) e (52b) podem cooperar com meios retentores |
Country Status (7)
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62234910A (ja) * | 1986-04-07 | 1987-10-15 | Meiki Co Ltd | デイスクの射出成形装置 |
| US4888956A (en) * | 1987-01-16 | 1989-12-26 | Roux Murray Pieter W Le | Cryogenic apparatus and cryogenic methods |
| JPH0232151A (ja) * | 1988-07-21 | 1990-02-01 | Diafoil Co Ltd | ポリエステル組成物 |
| DE69026754T2 (de) * | 1989-01-10 | 1996-11-14 | Teijin Ltd | Aromatischer polyesterfilm und verfahren zur herstellung |
| DE9104344U1 (de) * | 1991-04-10 | 1991-08-08 | Institut für Genetik und Kulturpflanzenforschung, O-4325 Gatersleben | Kühleinrichtung zur Probenvorbereitung für ein Elektronenmikroskop |
| AT403096B (de) * | 1992-09-08 | 1997-11-25 | Sitte Hellmuth | Verfahren und vorrichtung zur vorbereitung mikroskopischer, insbesondere elektronenmikroskopischer präparate für die schnittpräparation |
| AT403097B (de) * | 1992-09-08 | 1997-11-25 | Sitte Hellmuth | Vorrichtung zur entwässerung und/oder einbettung von proben |
| JP2521397B2 (ja) * | 1992-09-30 | 1996-08-07 | 東洋紡績株式会社 | ポリエステル組成物 |
| US5309722A (en) * | 1992-11-06 | 1994-05-10 | Harsco Corporation | Temperature control system for liquid nitrogen refrigerator |
| US5893269A (en) * | 1997-06-12 | 1999-04-13 | Yale University | Crystal freezing apparatus |
| JP2005098702A (ja) * | 1999-10-07 | 2005-04-14 | Seitai Kagaku Kenkyusho:Kk | 生物組織試料作製方法および器具 |
| US6871700B2 (en) * | 2000-11-17 | 2005-03-29 | G & H Technologies Llc | Thermal flux regulator |
| DE10146371A1 (de) * | 2001-09-20 | 2003-04-17 | Georg S Wengler | Verfahren zur Gewinnung menschlicher oder tierischer Stammzellen sowie Anlage zur Durchführung des Verfahrens |
| US7015568B2 (en) * | 2003-08-21 | 2006-03-21 | Texas Instruments Incorporated | System for ultraviolet atmospheric seed layer remediation |
| DE102004041965B4 (de) * | 2004-08-31 | 2009-08-13 | Leica Mikrosysteme Gmbh | Vorrichtung und Verfahren zur Gefriersubstitution und Einbettung von biologischen Proben |
| DE102004055148B4 (de) * | 2004-11-16 | 2006-11-09 | Leica Mikrosysteme Gmbh | Vorrichtung für die Gefrier- oder Tieftemperatursubstitution |
| DE102005003286B4 (de) * | 2005-01-25 | 2009-07-02 | Leica Mikrosysteme Gmbh | Behälter für eine Vorrichtung zur automatisierten Gefrier- oder Tieftemperatursubstitution |
| DE102005003284B4 (de) | 2005-01-25 | 2022-05-05 | Leica Mikrosysteme Gmbh | Dewar-Gefäß zur automatisierten Gefrier- oder Tieftemperatursubstitution |
| EP2008037A2 (en) * | 2006-03-30 | 2008-12-31 | Cornell Research Foundation, Inc. | System and method for increased cooling rates in rapid cooling of small biological samples |
| MX2008014784A (es) | 2006-05-19 | 2009-01-30 | Mary Kay Inc | Compuestos de glicerilo y acido glicolico. |
| JP5584153B2 (ja) * | 2011-02-28 | 2014-09-03 | 大陽日酸株式会社 | 予備凍結器 |
| US9089131B2 (en) | 2013-03-12 | 2015-07-28 | Mary Kay Inc. | Preservative system |
| WO2015152385A1 (ja) * | 2014-04-03 | 2015-10-08 | 株式会社日立ハイテクノロジーズ | クライオステーションシステム |
| CN107003065B (zh) * | 2014-11-13 | 2021-03-30 | 徕卡显微系统有限公司 | 带有用于冷冻的样本的容器的冷冻机 |
| US20190104594A1 (en) * | 2017-10-02 | 2019-04-04 | Worthington Industries, Inc. | Light device for dewar |
| FR3068769B1 (fr) * | 2018-03-13 | 2021-01-01 | Air Liquide | " dispositif et procede de refroidissement a temperature cryogenique" |
| US12151245B2 (en) * | 2020-11-20 | 2024-11-26 | Fred Hutchinson Cancer Research Center | Bio-specimen refrigeration system |
| US11808679B2 (en) * | 2022-01-27 | 2023-11-07 | Expresslo Llc | Method and apparatus for cryogenic and environmental controlled specimen handling |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2214009A (en) * | 1937-08-26 | 1940-09-10 | Jr Carl F Boester | Cooling apparatus for cooling beverages and the like |
| US3027734A (en) * | 1960-03-18 | 1962-04-03 | Reflectotherm Inc | Chilling and freezing systems |
| US4232453A (en) * | 1978-09-25 | 1980-11-11 | C. Reichert Optische Werke, Ag | Device for freeze drying and synthetic resin impregnation when necessary of small biological objects for electron microscopic examination |
| DE2944464A1 (de) * | 1979-11-03 | 1981-05-14 | C. Reichert Optische Werke Ag, Wien | Einrichtung zur kryosubstitution kleiner biologischer objekte fuer mikroskopische, insbesondere elektronenmikroskopische untersuchungen |
| NL7908379A (nl) * | 1979-11-16 | 1981-06-16 | Heesen T J | Inrichting voor het met behulp van lucht of gas behandelen van korrelvormig materiaal. |
| DE3042578A1 (de) * | 1980-11-12 | 1982-06-24 | C. Reichert Optische Werke Ag, Wien | Inkubationseinrichtung zur fixation, entwaesserung und einbettung biologischer objekte fuer mikroskopische, insbesondere elektronenmikroskopische untersuchungen |
| US4429542A (en) * | 1981-08-10 | 1984-02-07 | Hoxan Corporation | Method of freezing fertilized ova, spermatozoa or the like and apparatus therefor |
| DE3332586C2 (de) * | 1983-09-09 | 1986-03-20 | C. Reichert Optische Werke Ag, Wien | Vorrichtung zum Vermeiden des Verspritzens flüssiger Kühlmedien beim schnellen Einbringen biologisch-medizinischer Objekte in ein Kühlbad im Rahmen einer Immersions-Kryofixation |
| DE3234457C2 (de) * | 1982-09-17 | 1984-09-20 | C. Reichert Optische Werke Ag, Wien | Kühlbad zum raschen Abkühlen von Proben, insbesondere zur Kryofixation biologischer Objekte für eine nachfolgende licht- oder elektronenoptische Untersuchung |
| US4471629A (en) * | 1983-05-31 | 1984-09-18 | Mount Carmel Research And Education Corporation | Method of freezing and transplant of kidneys and apparatus |
| US4530816A (en) * | 1983-06-15 | 1985-07-23 | Hamilton Farm | Method and device for cooling, preserving and safely transporting biological material |
-
1984
- 1984-07-12 DE DE19843425744 patent/DE3425744A1/de active Granted
-
1985
- 1985-07-08 US US06/752,988 patent/US4723420A/en not_active Expired - Fee Related
- 1985-07-10 AT AT85304928T patent/ATE39574T1/de not_active IP Right Cessation
- 1985-07-10 EP EP85304928A patent/EP0170450B1/en not_active Expired
- 1985-07-11 PT PT80797A patent/PT80797B/pt unknown
- 1985-07-11 ES ES545105A patent/ES8604690A1/es not_active Expired
- 1985-07-12 JP JP60152575A patent/JPH0726895B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6140538A (ja) | 1986-02-26 |
| JPH0726895B2 (ja) | 1995-03-29 |
| DE3425744A1 (de) | 1986-01-16 |
| ES8604690A1 (es) | 1986-02-01 |
| ATE39574T1 (de) | 1989-01-15 |
| PT80797A (en) | 1985-08-01 |
| US4723420A (en) | 1988-02-09 |
| EP0170450A1 (en) | 1986-02-05 |
| DE3425744C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-10-27 |
| ES545105A0 (es) | 1986-02-01 |
| EP0170450B1 (en) | 1988-12-28 |
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