NZ523884A - Process for isolating PUFAs from microbial cells - Google Patents
Process for isolating PUFAs from microbial cellsInfo
- Publication number
- NZ523884A NZ523884A NZ523884A NZ52388401A NZ523884A NZ 523884 A NZ523884 A NZ 523884A NZ 523884 A NZ523884 A NZ 523884A NZ 52388401 A NZ52388401 A NZ 52388401A NZ 523884 A NZ523884 A NZ 523884A
- Authority
- NZ
- New Zealand
- Prior art keywords
- oil
- process according
- pufa
- microbial
- cells
- Prior art date
Links
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 50
- 230000000813 microbial effect Effects 0.000 title claims abstract description 39
- 210000004027 cell Anatomy 0.000 claims abstract description 61
- 210000002421 cell wall Anatomy 0.000 claims abstract description 34
- 239000002904 solvent Substances 0.000 claims abstract description 21
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 238000005119 centrifugation Methods 0.000 claims abstract description 12
- 238000000265 homogenisation Methods 0.000 claims abstract description 9
- 230000000593 degrading effect Effects 0.000 claims abstract description 7
- 239000003921 oil Substances 0.000 claims description 63
- 238000000926 separation method Methods 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 12
- 239000000411 inducer Substances 0.000 claims description 8
- 239000004094 surface-active agent Substances 0.000 claims description 7
- 241000907999 Mortierella alpina Species 0.000 claims description 6
- 239000010779 crude oil Substances 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 241000235648 Pichia Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 241000228212 Aspergillus Species 0.000 claims description 2
- 241000235548 Blakeslea Species 0.000 claims description 2
- 241001480508 Entomophthora Species 0.000 claims description 2
- 241000235400 Phycomyces Species 0.000 claims description 2
- 241000206618 Porphyridium Species 0.000 claims description 2
- 241000186429 Propionibacterium Species 0.000 claims description 2
- 241000235070 Saccharomyces Species 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 18
- 230000004151 fermentation Effects 0.000 abstract description 18
- 239000012071 phase Substances 0.000 abstract description 10
- 238000000605 extraction Methods 0.000 abstract description 8
- 239000008346 aqueous phase Substances 0.000 abstract description 6
- 230000002255 enzymatic effect Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 238000009835 boiling Methods 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 28
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 15
- 229940114079 arachidonic acid Drugs 0.000 description 14
- 235000021342 arachidonic acid Nutrition 0.000 description 14
- 150000002632 lipids Chemical class 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- -1 fatty acid ester Chemical class 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 150000003904 phospholipids Chemical class 0.000 description 6
- 241000195493 Cryptophyta Species 0.000 description 5
- 241000233866 Fungi Species 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 150000003626 triacylglycerols Chemical class 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 241000199912 Crypthecodinium cohnii Species 0.000 description 4
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 4
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 4
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 241000199913 Crypthecodinium Species 0.000 description 3
- 229930182558 Sterol Natural products 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000000306 component Substances 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 235000021588 free fatty acids Nutrition 0.000 description 3
- VZCCETWTMQHEPK-QNEBEIHSSA-N gamma-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCC(O)=O VZCCETWTMQHEPK-QNEBEIHSSA-N 0.000 description 3
- 230000002934 lysing effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000010297 mechanical methods and process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 238000000053 physical method Methods 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 150000003432 sterols Chemical class 0.000 description 3
- 235000003702 sterols Nutrition 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000199914 Dinophyceae Species 0.000 description 2
- 102000004882 Lipase Human genes 0.000 description 2
- 108090001060 Lipase Proteins 0.000 description 2
- 239000004367 Lipase Substances 0.000 description 2
- 102000003820 Lipoxygenases Human genes 0.000 description 2
- 108090000128 Lipoxygenases Proteins 0.000 description 2
- 241000233675 Thraustochytrium Species 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 2
- 239000012298 atmosphere Substances 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- HOBAELRKJCKHQD-QNEBEIHSSA-N dihomo-γ-linolenic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/CCCCCCC(O)=O HOBAELRKJCKHQD-QNEBEIHSSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 235000019421 lipase Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000007670 refining Methods 0.000 description 2
- ADHNUPOJJCKWRT-JLXBFWJWSA-N (2e,4e)-octadeca-2,4-dienoic acid Chemical compound CCCCCCCCCCCCC\C=C\C=C\C(O)=O ADHNUPOJJCKWRT-JLXBFWJWSA-N 0.000 description 1
- HOBAELRKJCKHQD-UHFFFAOYSA-N (8Z,11Z,14Z)-8,11,14-eicosatrienoic acid Natural products CCCCCC=CCC=CCC=CCCCCCCC(O)=O HOBAELRKJCKHQD-UHFFFAOYSA-N 0.000 description 1
- JBYXPOFIGCOSSB-GOJKSUSPSA-N 9-cis,11-trans-octadecadienoic acid Chemical compound CCCCCC\C=C\C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-GOJKSUSPSA-N 0.000 description 1
- 241001465318 Aspergillus terreus Species 0.000 description 1
- 241000235553 Blakeslea trispora Species 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- BDCFUHIWJODVNG-UHFFFAOYSA-N Desmosterol Natural products C1C=C2CC(O)C=CC2(C)C2C1C1CCC(C(C)CCC(CC)C(C)C)C1(C)CC2 BDCFUHIWJODVNG-UHFFFAOYSA-N 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 description 1
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 description 1
- 241000235575 Mortierella Species 0.000 description 1
- 241000235388 Mucorales Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241000197220 Pythium insidiosum Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 150000001350 alkyl halides Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000010385 ascorbyl palmitate Nutrition 0.000 description 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940108924 conjugated linoleic acid Drugs 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- AVSXSVCZWQODGV-DPAQBDIFSA-N desmosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@@H](CCC=C(C)C)C)[C@@]1(C)CC2 AVSXSVCZWQODGV-DPAQBDIFSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 1
- 229940090949 docosahexaenoic acid Drugs 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 125000004494 ethyl ester group Chemical group 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 108010002430 hemicellulase Proteins 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 230000004130 lipolysis Effects 0.000 description 1
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- DTOSIQBPPRVQHS-UHFFFAOYSA-N α-Linolenic acid Chemical compound CCC=CCC=CCC=CCCCCCCCC(O)=O DTOSIQBPPRVQHS-UHFFFAOYSA-N 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Polymers & Plastics (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00306601A EP1178118A1 (en) | 2000-08-02 | 2000-08-02 | Isolation of microbial oils |
| PCT/EP2001/008903 WO2002010423A2 (en) | 2000-08-02 | 2001-08-01 | Isolation of microbial oils |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ523884A true NZ523884A (en) | 2004-09-24 |
Family
ID=8173160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ523884A NZ523884A (en) | 2000-08-02 | 2001-08-01 | Process for isolating PUFAs from microbial cells |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US7431952B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP1178118A1 (cg-RX-API-DMAC7.html) |
| JP (4) | JP2004504849A (cg-RX-API-DMAC7.html) |
| KR (6) | KR101515317B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN1282745C (cg-RX-API-DMAC7.html) |
| AT (1) | ATE470719T1 (cg-RX-API-DMAC7.html) |
| AU (2) | AU2001293711B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR0112942A (cg-RX-API-DMAC7.html) |
| CA (2) | CA2417571C (cg-RX-API-DMAC7.html) |
| DE (1) | DE60142339D1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK1305440T3 (cg-RX-API-DMAC7.html) |
| EA (1) | EA006979B1 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2357614T3 (cg-RX-API-DMAC7.html) |
| IL (2) | IL154146A0 (cg-RX-API-DMAC7.html) |
| MX (1) | MXPA03000878A (cg-RX-API-DMAC7.html) |
| NO (1) | NO20030510L (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ523884A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2002010423A2 (cg-RX-API-DMAC7.html) |
| ZA (1) | ZA200300787B (cg-RX-API-DMAC7.html) |
Families Citing this family (112)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080007279A (ko) * | 2000-01-19 | 2008-01-17 | 마텍 바이오싸이언스스 코포레이션 | 무용매 추출 방법 |
| ES2324640T3 (es) * | 2001-03-09 | 2009-08-12 | Societe Des Produits Nestle S.A. | Aceite que contiene un o unos acido(s) graso(s) poliin-saturado(s) de cadena larga resultante de una biomasa, procedimiento de preparacion, alimento, composicion nutricional, cosmetica o farmaceutica que lo contiene. |
| EP2921155B1 (en) | 2002-05-03 | 2020-01-08 | DSM IP Assets B.V. | Methods for producing high-quality lipids by enzymatic liberation from biomass |
| WO2004001021A1 (en) | 2002-06-19 | 2003-12-31 | Dsm Ip Assets B.V. | Pasteurisation process for microbial cells and microbial oil |
| DK1396533T3 (da) * | 2002-09-04 | 2009-01-12 | Nestec Sa | Fremgangsmåde til fremstilling af en olie indeholdende en eller flere polyumættede langkædede fedtsyrer ud fra biomasse, næringsmiddel samt nærende, kosmetisk eller farmaceutisk sammensætning indeholdende denne |
| US7601858B2 (en) * | 2004-08-17 | 2009-10-13 | Gs Cleantech Corporation | Method of processing ethanol byproducts and related subsystems |
| WO2006046943A2 (en) * | 2004-10-22 | 2006-05-04 | Martek Biosciences Corporation | Methods for producing lipids by liberation from biomass |
| DE102005003624A1 (de) * | 2005-01-26 | 2006-07-27 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Herstellung und Anwendung eines antioxidativ wirksamen Extraktes aus Crypthecodinium sp. |
| US9108140B2 (en) * | 2005-03-16 | 2015-08-18 | Gs Cleantech Corporation | Method and systems for washing ethanol production byproducts to improve oil recovery |
| EP2447356B1 (en) | 2005-06-07 | 2016-04-20 | DSM Nutritional Products AG | Eukaryotic microorganisms for producing lipids and antioxidants |
| EP1903883A4 (en) * | 2005-07-01 | 2010-06-23 | Martek Biosciences Corp | OIL PRODUCT CONTAINING MULTIPLE UNSATURATED FATTY ACIDS AND ITS USES AND MANUFACTURING |
| US8003369B2 (en) * | 2005-11-29 | 2011-08-23 | Sanyo Chemical Industries, Ltd. | Bacteriolytic agent |
| ES2572833T3 (es) | 2006-08-01 | 2016-06-02 | Dsm Nutritional Products Ag | Proceso de producción de aceite microbiano que contiene ácidos grasos poliinsaturados |
| US7905930B2 (en) | 2006-12-29 | 2011-03-15 | Genifuel Corporation | Two-stage process for producing oil from microalgae |
| US8476059B2 (en) | 2007-06-01 | 2013-07-02 | Solazyme, Inc. | Sucrose feedstock utilization for oil-based fuel manufacturing |
| US8343753B2 (en) * | 2007-11-01 | 2013-01-01 | Wake Forest University School Of Medicine | Compositions, methods, and kits for polyunsaturated fatty acids from microalgae |
| US8043496B1 (en) | 2008-03-18 | 2011-10-25 | Peter Allen Schuh | System for extracting oil from algae |
| EP2145942A1 (de) * | 2008-07-15 | 2010-01-20 | Lonza Ltd. | Verfahren zur Isolierung von Ölen aus Zellen und Biomasse |
| US8815570B2 (en) * | 2008-10-02 | 2014-08-26 | Feyecon B.V. | Microalgae extract containing omega 3-polyunsaturated fatty acids and method for extracting oil from micro-organisms |
| US20160324167A1 (en) | 2008-10-14 | 2016-11-10 | Terravia Holdings, Inc. | Novel microalgal food compositions |
| US20100303989A1 (en) | 2008-10-14 | 2010-12-02 | Solazyme, Inc. | Microalgal Flour |
| CA2745129C (en) | 2008-11-28 | 2019-05-07 | Solazyme, Inc. | Production of tailored oils in heterotrophic microorganisms |
| US8207363B2 (en) * | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| CN101817738B (zh) * | 2009-11-13 | 2013-08-14 | 厦门汇盛生物有限公司 | 一种从藻类和真菌细胞中破壁提取dha的方法 |
| JP5920890B2 (ja) | 2010-01-19 | 2016-05-18 | ディーエスエム アイピー アセッツ ビー．ブイ． | エイコサペンタエン酸生成微生物、脂肪酸組成物、ならびにそれらを作る方法およびそれらの使用 |
| US8187861B1 (en) | 2010-01-26 | 2012-05-29 | Allen John Schuh | Phosphate removal-recovery and biofuel feedstock system |
| CN101805670A (zh) * | 2010-04-09 | 2010-08-18 | 上海中器环保科技有限公司 | 一种微生物柴油的制备方法 |
| JP5868962B2 (ja) | 2010-05-28 | 2016-02-24 | ソラザイム, インコーポレイテッドSolazyme Inc | 組み換え従属栄養性微生物から産生された用途に応じた油 |
| EP3617318A1 (en) | 2010-06-01 | 2020-03-04 | DSM IP Assets B.V. | Extraction of lipid from cells and products therefrom |
| NL2004832C2 (en) * | 2010-06-07 | 2011-12-08 | Evodos B V | Separating biomass from an aqueous medium. |
| US8906236B2 (en) | 2010-07-26 | 2014-12-09 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds and nutrients from biomass |
| US9028696B2 (en) | 2010-07-26 | 2015-05-12 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds from biomass |
| BR112013002003A2 (pt) | 2010-07-26 | 2019-09-24 | Sapphire Energy Inc | processo para recuperação de compostos oleaginosos de biomassa |
| BR112013006493A2 (pt) | 2010-09-21 | 2016-07-26 | Shell Int Research | processo para separar uma mistura contendo um óleo microbiano e uma substância microbiana |
| MX2012012250A (es) * | 2010-10-18 | 2013-03-05 | Originoil Inc | Sistemas, aparatos, y metodos para extraer lipidos no polares de una lechada acuosa de algas y lipidos producidos de la misma. |
| WO2012061647A2 (en) | 2010-11-03 | 2012-05-10 | Solazyme, Inc. | Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods |
| US20120329138A1 (en) | 2010-12-23 | 2012-12-27 | Shell Oil Company | Process for separation of a mixture containing a microbial substance and a liquid |
| MX351063B (es) | 2011-02-02 | 2017-09-29 | Terravia Holdings Inc | Aceites adaptados producidos a partir de microorganismos oleaginosos recombinantes. |
| CA3026436A1 (en) * | 2011-02-11 | 2012-08-16 | E. I. Du Pont De Nemours And Company | Method for obtaining a lipid-containing composition from microbial biomass |
| WO2012109543A1 (en) * | 2011-02-11 | 2012-08-16 | E. I. Du Pont De Nemours And Company | Method for forming and extracting solid pellets comprising oil-containing microbes |
| KR20140033378A (ko) | 2011-05-06 | 2014-03-18 | 솔라짐, 인코포레이티드 | 자일로오스를 대사시키는 유전자 조작된 미생물 |
| FR2975705B1 (fr) | 2011-05-27 | 2014-12-26 | Roquette Freres | Procede d'extraction du squalene a partir de microalgues |
| EP2952183A1 (en) | 2011-07-21 | 2015-12-09 | DSM IP Assets B.V. | Fatty acid compositions |
| US8986977B2 (en) * | 2011-12-30 | 2015-03-24 | Alliance For Sustainable Energy, Llc | Disruption of cell walls for enhanced lipid recovery |
| US12268718B2 (en) | 2012-01-16 | 2025-04-08 | Labyrinth Holdings Llc | Control of cellular redox levels |
| US20140086849A1 (en) | 2012-09-21 | 2014-03-27 | Elizabeth McKenna | Naturally-Occurring CpG Oligonucleotide Compositions and Therapeutic Applications Thereof |
| EP2804613A4 (en) | 2012-01-16 | 2015-12-09 | Elizabeth Mckenna | COMPOSITIONS AND METHODS FOR THE TREATMENT OF HEPATORY ILLNESSES AND DISORDERS |
| JP6222963B2 (ja) | 2012-04-10 | 2017-11-01 | 花王株式会社 | 脂肪酸エステルの製造方法 |
| CA2870364A1 (en) | 2012-04-18 | 2013-10-24 | Solazyme, Inc. | Recombinant microbes with modified fatty acid synthetic pathway enzymes and uses thereof |
| KR101432277B1 (ko) * | 2012-05-22 | 2014-08-21 | 한국과학기술원 | 양이온성 고분자를 이용한 미세조류로부터 지질의 추출방법 및 추출된 지질을 이용한 바이오디젤 제조방법 |
| CN102702777B (zh) * | 2012-06-07 | 2014-03-19 | 北京林业大学 | 一种酶法水解从植物原料中提取水溶性类胡萝卜素的方法 |
| WO2014041985A1 (ja) * | 2012-09-11 | 2014-03-20 | Dic株式会社 | 液晶表示装置 |
| CA2895684C (en) * | 2012-12-19 | 2020-04-14 | Buckman Laboratories International, Inc. | Methods and systems for bio-oil recovery and separation aids therefor |
| US10098371B2 (en) | 2013-01-28 | 2018-10-16 | Solazyme Roquette Nutritionals, LLC | Microalgal flour |
| EP2762008A1 (de) * | 2013-02-05 | 2014-08-06 | Evonik Industries AG | Verbesserung der Bioverfügbarkeit von Wertstoffen aus Mikroorganismen durch Verwendung eines Rotor-Stator Systems für den Zellaufschluss |
| WO2014141098A1 (en) | 2013-03-13 | 2014-09-18 | Dsm Nutritional Products Ag | Engineering microorganisms |
| KR102023756B1 (ko) * | 2013-05-14 | 2019-09-23 | 에스케이이노베이션 주식회사 | 신규 트라우스토키트리드〔Ｔｈｒａｕｓｔｏｃｈｙｔｒｉｄ〕 계 미세조류 트라우스토키트리움〔Ｔｈｒａｕｓｔｏｃｈｙｔｒｉｕｍ〕ｓｐ．ＬＡ６〔ＫＣＴＣ１２３８９ＢＰ〕및 이를 이용한 바이오오일의 생산방법 |
| CN104293475B (zh) * | 2013-07-19 | 2017-07-25 | 中国石油化工股份有限公司 | 一种从产油微生物中提取油脂的方法 |
| FR3009619B1 (fr) | 2013-08-07 | 2017-12-29 | Roquette Freres | Compositions de biomasse de microalgues riches en proteines de qualite sensorielle optimisee |
| EP3052636A2 (en) | 2013-10-04 | 2016-08-10 | Solazyme, Inc. | Tailored oils |
| ES2875965T3 (es) | 2013-12-04 | 2021-11-11 | Nippon Suisan Kaisha Ltd | Aceite microbiano que contiene ácido dihomo-gamma-linolénico y biomasa microbiana que contiene ácido dihomo-gamma-linolénico |
| CA2934509C (en) | 2013-12-20 | 2022-05-03 | Neil Francis Leininger | Processes for obtaining microbial oil from microbial cells |
| KR102435268B1 (ko) * | 2013-12-20 | 2022-08-22 | 디에스엠 뉴트리셔널 프라덕츠 아게 | 미생물로부터 오일을 회수하는 방법 |
| PT3626806T (pt) | 2013-12-20 | 2024-07-31 | Mara Renewables Corp | Métodos para recuperar óleo de microrganismos |
| KR102435269B1 (ko) * | 2013-12-20 | 2022-08-22 | 디에스엠 아이피 어셋츠 비.브이. | 미생물 세포로부터의 미생물 오일의 수득 방법 |
| US11124736B2 (en) * | 2013-12-20 | 2021-09-21 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| SG11201605061TA (en) * | 2013-12-20 | 2016-08-30 | Dsm Ip Assets Bv | Process for extracting lipids for use in production of biofuels |
| AR098890A1 (es) | 2013-12-20 | 2016-06-22 | Dsm Ip Assets Bv | Proceso para obtener aceite microbiano a partir de células microbianas |
| AU2014369042B2 (en) * | 2013-12-20 | 2020-04-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| CN103789083B (zh) * | 2014-02-17 | 2016-01-20 | 南京工业大学大丰海洋产业研究院 | 一种真菌油脂提取方法 |
| US9969990B2 (en) | 2014-07-10 | 2018-05-15 | Corbion Biotech, Inc. | Ketoacyl ACP synthase genes and uses thereof |
| CN104356097B (zh) * | 2014-10-20 | 2016-06-29 | 中国科学院广州能源研究所 | 一种微生物油脂基环氧化物的制备方法 |
| CN104513704B (zh) * | 2014-12-11 | 2018-01-12 | 湖北福星生物科技有限公司 | 一种无腥味dha油脂的提取方法 |
| CN104479862A (zh) * | 2014-12-31 | 2015-04-01 | 嘉必优生物工程（武汉）有限公司 | 一种提取微生物油脂的方法 |
| AR104042A1 (es) | 2015-03-26 | 2017-06-21 | Mara Renewables Corp | Producción de alta densidad de biomasa y aceite utilizando glicerol en bruto |
| US10815513B2 (en) | 2015-05-29 | 2020-10-27 | Cargill, Incorporated | Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom |
| CN107614677A (zh) | 2015-05-29 | 2018-01-19 | 嘉吉公司 | 用于使用多阶段进料生产甜菊醇糖苷的发酵方法 |
| EP3302097B1 (en) | 2015-05-29 | 2022-03-23 | Cargill, Incorporated | Heat treatment to produce glycosides |
| JP6977231B2 (ja) | 2015-07-13 | 2021-12-08 | マラ リニューアブルズ コーポレーション | Ｃ５有機炭素の微生物による代謝の増強 |
| WO2017024313A1 (en) | 2015-08-06 | 2017-02-09 | Cargill, Incorporated | Fermentation methods for producing steviol glycosides |
| US10377792B2 (en) | 2016-03-16 | 2019-08-13 | The Texas A&M University System | Moisture displacement and simultaneous migration of surface-functionalized algae from water to an extraction solvent using ionic polyelectrolytes |
| US10851395B2 (en) | 2016-06-10 | 2020-12-01 | MARA Renewables Corporation | Method of making lipids with improved cold flow properties |
| US11419350B2 (en) | 2016-07-01 | 2022-08-23 | Corbion Biotech, Inc. | Feed ingredients comprising lysed microbial cells |
| KR102405390B1 (ko) * | 2016-07-13 | 2022-06-03 | 에보닉 오퍼레이션스 게엠베하 | 용해된 지질을 함유하는 바이오매스로부터 지질을 분리하는 방법 |
| RU2744913C2 (ru) * | 2016-12-27 | 2021-03-17 | Эвоник Оперейшенс ГмбХ | Способ выделения липидов из липидсодержащей биомассы |
| WO2018122057A1 (en) * | 2016-12-27 | 2018-07-05 | Evonik Degussa Gmbh | Method of isolating lipids from a lipids containing biomass |
| CN108570484A (zh) * | 2017-03-07 | 2018-09-25 | 武汉普赛特膜技术循环利用有限公司 | 一种使用三次发酵法由藻发酵液中纯化富集dha油脂的方法 |
| US10787625B2 (en) * | 2017-07-12 | 2020-09-29 | Bunge Global Innovation Llc | Process for extraction of oil from algal biomass |
| ES2916462T3 (es) * | 2017-08-10 | 2022-07-01 | Dsm Ip Assets Bv | Proceso de doble centrifugación para la purificación de aceite nutritivo |
| WO2019034354A1 (en) | 2017-08-17 | 2019-02-21 | Evonik Degussa Gmbh | ENHANCED PRODUCTION OF LIPIDS BY LIMITING AT LEAST TWO SOURCES OF NUTRIENT LIMITING |
| CN111356767A (zh) | 2017-08-17 | 2020-06-30 | 赢创运营有限公司 | 通过限制至少两种限制性营养源增强脂质的产生 |
| EP3470502A1 (en) | 2017-10-13 | 2019-04-17 | Evonik Degussa GmbH | Method of separating lipids from a lysed lipids containing biomass |
| CN107904016B (zh) * | 2017-11-10 | 2021-04-20 | 海南华研胶原科技股份有限公司 | 一种红藻精油及其制备方法 |
| EP3527664A1 (en) | 2018-02-15 | 2019-08-21 | Evonik Degussa GmbH | Method of isolating lipids from a lipids containing biomass |
| WO2019122030A1 (en) * | 2017-12-22 | 2019-06-27 | Dsm Ip Assets B.V. | Method of separating lipids from a lysed lipids containing biomass |
| DE202018000893U1 (de) * | 2018-02-19 | 2019-05-22 | Dorothea Jürgens | Vorrichtung zur umwelt-und energieschonenden Veraschung von Leichen und Leichenteilen unter vorheriger Separierung der flüssigen Anteile und Verwendung der brennbaren Fraktion zur energetischen Weiterverwendung in und auch ausserhalb der Vorrichtung |
| EP3536800B1 (en) | 2018-03-09 | 2021-08-04 | TU München | Extraction of renewable triglycerides from oleaginous microorganisms |
| EP3775118A4 (en) * | 2018-03-30 | 2022-03-09 | DSM IP Assets B.V. | Method of reducing emulsion by broth washing |
| DK3794097T3 (da) | 2018-05-15 | 2024-12-16 | Evonik Operations Gmbh | Fremgangsmåde til isolering af lipider fra en lipid-indeholdende biomasse ved hjælp af hydrofob silica |
| BR112020023222A2 (pt) | 2018-05-15 | 2021-03-23 | Evonik Operations Gmbh | método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão |
| CN108753457A (zh) * | 2018-08-03 | 2018-11-06 | 梁云 | 提高微生物油脂稳定性和安全性的方法 |
| CN108753458A (zh) * | 2018-08-03 | 2018-11-06 | 梁云 | 提高微生物油脂稳定性和安全性的精炼工艺 |
| FR3085825B1 (fr) | 2018-09-14 | 2021-07-16 | Fermentalg | Huile de microorganismes riche en acide docosahexaenoique |
| WO2020186128A1 (en) * | 2019-03-14 | 2020-09-17 | Dsm Ip Assets B.V. | Methods of obtaining lipids from a microbial cell composition |
| CN111363614A (zh) * | 2019-10-10 | 2020-07-03 | 润科生物工程（福建）有限公司 | 一种非溶剂式提取花生四烯酸油脂的方法 |
| CN110760369A (zh) * | 2019-11-08 | 2020-02-07 | 润科生物工程（福建）有限公司 | 一种纯物理提取花生四烯酸油脂的方法 |
| BR112022019834A2 (pt) | 2020-04-03 | 2022-12-06 | Mara Renewables Corp | Óleo microbiano, e, método de compor uma biomassa |
| CN111518611A (zh) * | 2020-04-28 | 2020-08-11 | 上海容邦企业集团有限公司 | 沙棘果油复合物及制备方法 |
| CN113684087B (zh) * | 2020-05-18 | 2024-04-19 | 嘉必优生物技术（武汉）股份有限公司 | 一种微生物油脂无溶剂提取方法及所得微生物油脂 |
| EP3933016A1 (en) * | 2020-06-30 | 2022-01-05 | Evonik Operations GmbH | Method of isolating lipids from a lipids containing biomass |
| FR3111912B1 (fr) | 2020-06-24 | 2025-10-03 | Fermentalg | Procédé de culture de microorganismes pour l’accumulation de lipides |
| KR102795205B1 (ko) * | 2022-01-27 | 2025-04-10 | 씨제이제일제당 (주) | 냉각 공정을 이용한 오일 회수율이 향상된 바이오오일 추출 방법 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB808128A (en) * | 1955-12-01 | 1959-01-28 | Nat Res Dev | A method of increasing the fatty contents of such micro-organisms as yeasts, bacteria and moulds |
| DE3248167A1 (de) * | 1982-12-27 | 1984-06-28 | Wintershall Ag, 3100 Celle | Trehaloselipidtetraester |
| JPS61170397A (ja) * | 1985-01-22 | 1986-08-01 | Agency Of Ind Science & Technol | モルテイエレラ属糸状菌体の多段抽出処理方法 |
| JPS62278987A (ja) * | 1986-05-26 | 1987-12-03 | Kanegafuchi Chem Ind Co Ltd | 酵素反応生成物の回収方法 |
| DK199887D0 (da) * | 1987-04-15 | 1987-04-15 | Danisco Bioteknologi As | Gaerstamme |
| JPS63304990A (ja) * | 1987-06-04 | 1988-12-13 | Meiji Seika Kaisha Ltd | 菌体内有効成分の抽出方法 |
| JPH0198494A (ja) * | 1987-10-09 | 1989-04-17 | Agency Of Ind Science & Technol | バイオリアクター |
| KR900004067A (ko) * | 1988-08-02 | 1990-03-27 | 김용원 | 100v/220v 겸용 코넥터, 콘센트 및 전압선택 플러그 |
| US5130242A (en) * | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| FR2656874B1 (fr) * | 1990-01-11 | 1992-04-03 | Commissariat Energie Atomique | Procede de production et d'extraction d'anti-oxydants a partir d'une culture de micro-organismes et photobioreacteur pour la mise en óoeuvre de ce procede. |
| US5658767A (en) * | 1991-01-24 | 1997-08-19 | Martek Corporation | Arachidonic acid and methods for the production and use thereof |
| FR2686619B1 (fr) | 1992-01-28 | 1995-07-13 | Commissariat Energie Atomique | Procede de production selective de lipides poly-insatures a partir d'une culture de micro-algues du type porphyridium et cuve utilisee dans ce procede. |
| DE4219360C2 (de) * | 1992-06-12 | 1994-07-28 | Milupa Ag | Verfahren zur Gewinnung von Lipiden mit einem hohen Anteil von langkettig-hochungesättigten Fettsäuren |
| EP0776356B1 (de) * | 1994-08-16 | 1999-06-30 | Dr. Frische GmbH | Verfahren zur gewinnung von nicht wasserlöslichen, nativen produkten aus nativen stoffgemengen mit hilfe der zentrifugalkraft |
| KR100212644B1 (ko) * | 1994-10-29 | 1999-08-02 | 토니헬샴 | 유압식 건설기계의 엔진회전수 제어장치 및 제어방법 |
| US5583019A (en) * | 1995-01-24 | 1996-12-10 | Omegatech Inc. | Method for production of arachidonic acid |
| ES2130515T3 (es) | 1995-05-04 | 1999-07-01 | Nestle Sa | Procedimiento para fraccionar acidos grasos. |
| JPH099981A (ja) * | 1995-06-28 | 1997-01-14 | Sekiyu Sangyo Kasseika Center | 油水二相系微生物反応における油水分離方法 |
| GB9514649D0 (en) * | 1995-07-18 | 1995-09-13 | Zeneca Ltd | Extraction of triglycerides from microorganisms |
| EP2280062A3 (en) * | 1996-03-28 | 2011-09-28 | DSM IP Assets B.V. | Preparation of microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
| CN104017734A (zh) * | 1996-03-28 | 2014-09-03 | Dsmip资产有限公司 | 粒状微生物生物质的制备及其有价值的化合物的分离方法 |
| JP3343358B2 (ja) | 1996-07-23 | 2002-11-11 | ナガセケムテックス株式会社 | ドコサヘキサエン酸およびドコサペンタエン酸の製造方法 |
| AU7530598A (en) | 1997-05-02 | 1998-11-27 | Dsm N.V. | Isolation of carotenoid crystals from microbial biomass |
| JP3836231B2 (ja) * | 1997-10-17 | 2006-10-25 | 日本化学飼料株式会社 | ホタテガイ中腸腺から得られる高度不飽和脂肪酸含有油及びその製造方法 |
| EP1042451A4 (en) | 1997-12-23 | 2001-07-04 | Dcv Inc | LINOLEAT ISOMERASE |
| JP2000041684A (ja) * | 1998-07-29 | 2000-02-15 | Daicel Chem Ind Ltd | 新規なｄ−アミノアシラーゼおよびその製造方法、並びに該ｄ−アミノアシラーゼを利用したｄ−アミノ酸の製造方法 |
| JP2000135096A (ja) * | 1998-08-28 | 2000-05-16 | Tadayuki Imanaka | 界面活性剤、その生産方法およびその利用方法 |
| KR20080007279A (ko) * | 2000-01-19 | 2008-01-17 | 마텍 바이오싸이언스스 코포레이션 | 무용매 추출 방법 |
-
2000
- 2000-08-02 EP EP00306601A patent/EP1178118A1/en not_active Withdrawn
-
2001
- 2001-08-01 KR KR1020137023848A patent/KR101515317B1/ko not_active Expired - Lifetime
- 2001-08-01 EA EA200300212A patent/EA006979B1/ru not_active IP Right Cessation
- 2001-08-01 CN CNB018143016A patent/CN1282745C/zh not_active Expired - Lifetime
- 2001-08-01 AU AU2001293711A patent/AU2001293711B2/en not_active Expired
- 2001-08-01 KR KR20037001502A patent/KR20030059086A/ko not_active Ceased
- 2001-08-01 EP EP01974095.0A patent/EP1305440B2/en not_active Expired - Lifetime
- 2001-08-01 KR KR1020127027187A patent/KR101447912B1/ko not_active Expired - Lifetime
- 2001-08-01 JP JP2002516339A patent/JP2004504849A/ja active Pending
- 2001-08-01 KR KR1020107002710A patent/KR20100019579A/ko not_active Ceased
- 2001-08-01 DK DK01974095.0T patent/DK1305440T3/da active
- 2001-08-01 AU AU9371101A patent/AU9371101A/xx active Pending
- 2001-08-01 ES ES01974095T patent/ES2357614T3/es not_active Expired - Lifetime
- 2001-08-01 BR BR0112942-2A patent/BR0112942A/pt not_active Application Discontinuation
- 2001-08-01 KR KR1020117002571A patent/KR20110030661A/ko not_active Ceased
- 2001-08-01 KR KR1020147015585A patent/KR101648277B1/ko not_active Expired - Lifetime
- 2001-08-01 NZ NZ523884A patent/NZ523884A/en not_active IP Right Cessation
- 2001-08-01 US US10/343,861 patent/US7431952B2/en not_active Expired - Lifetime
- 2001-08-01 CA CA2417571A patent/CA2417571C/en not_active Expired - Lifetime
- 2001-08-01 CA CA2742152A patent/CA2742152C/en not_active Expired - Lifetime
- 2001-08-01 AT AT01974095T patent/ATE470719T1/de not_active IP Right Cessation
- 2001-08-01 MX MXPA03000878A patent/MXPA03000878A/es active IP Right Grant
- 2001-08-01 DE DE60142339T patent/DE60142339D1/de not_active Expired - Lifetime
- 2001-08-01 IL IL15414601A patent/IL154146A0/xx unknown
- 2001-08-01 WO PCT/EP2001/008903 patent/WO2002010423A2/en not_active Ceased
-
2003
- 2003-01-27 IL IL154146A patent/IL154146A/en active IP Right Grant
- 2003-01-29 ZA ZA200300787A patent/ZA200300787B/xx unknown
- 2003-01-31 NO NO20030510A patent/NO20030510L/no not_active Application Discontinuation
-
2011
- 2011-09-08 JP JP2011196284A patent/JP2012019794A/ja active Pending
-
2014
- 2014-02-21 JP JP2014031850A patent/JP2014138598A/ja active Pending
-
2016
- 2016-05-06 JP JP2016093361A patent/JP2016208974A/ja active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2742152C (en) | Isolation of microbial oils | |
| AU2001293711A1 (en) | Isolation of microbial oils | |
| US9408404B2 (en) | Method for producing lipids by liberation from biomass | |
| AU2014203384B2 (en) | Solventless extraction process | |
| WO2006046943A2 (en) | Methods for producing lipids by liberation from biomass | |
| HK1152490A (en) | High quality lipids and methods for producing by enzymatic liberation from biomass | |
| HK1154906B (en) | Solventless extraction process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) | ||
| ASS | Change of ownership |
Owner name: DSM IP ASSETS B.V., NL Free format text: OLD OWNER(S): DSM IP ASSETS B.V.; DSM N.V. |
|
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 7 YEARS UNTIL 01 AUG 2021 BY CPA GLOBAL Effective date: 20140620 |
|
| EXPY | Patent expired |