NO329876B1 - Fremgangsmate for overvaking av effektiviteten til en renseprosess ved fjerning av endogen plasminogenaktivator (PA) fra kinesisk hamsterovarie-(CHO)-celler, fra en prove som inneholder human tPA eller et tPA-molekyl hvori Thr103 fra human villtype-tPA er endret til Asn, Asn117 fra human villtype-tPA er endret til Gln og Lys-His-Arg-Arg fra human villype-tPA er endret til Ala-Ala-Ala-Ala i posisjon 296-299 - Google Patents

Fremgangsmate for overvaking av effektiviteten til en renseprosess ved fjerning av endogen plasminogenaktivator (PA) fra kinesisk hamsterovarie-(CHO)-celler, fra en prove som inneholder human tPA eller et tPA-molekyl hvori Thr103 fra human villtype-tPA er endret til Asn, Asn117 fra human villtype-tPA er endret til Gln og Lys-His-Arg-Arg fra human villype-tPA er endret til Ala-Ala-Ala-Ala i posisjon 296-299

Info

Publication number
NO329876B1
NO329876B1 NO20022122A NO20022122A NO329876B1 NO 329876 B1 NO329876 B1 NO 329876B1 NO 20022122 A NO20022122 A NO 20022122A NO 20022122 A NO20022122 A NO 20022122A NO 329876 B1 NO329876 B1 NO 329876B1
Authority
NO
Norway
Prior art keywords
tpa
ala
changed
human wild
arg
Prior art date
Application number
NO20022122A
Other languages
English (en)
Norwegian (no)
Other versions
NO20022122D0 (no
NO20022122L (no
Inventor
Yuan Xu
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of NO20022122D0 publication Critical patent/NO20022122D0/no
Publication of NO20022122L publication Critical patent/NO20022122L/no
Publication of NO329876B1 publication Critical patent/NO329876B1/no

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators
    • G01N2333/9726—Tissue plasminogen activator
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Signal Processing For Digital Recording And Reproducing (AREA)
  • Glass Compositions (AREA)
NO20022122A 1999-11-04 2002-05-03 Fremgangsmate for overvaking av effektiviteten til en renseprosess ved fjerning av endogen plasminogenaktivator (PA) fra kinesisk hamsterovarie-(CHO)-celler, fra en prove som inneholder human tPA eller et tPA-molekyl hvori Thr103 fra human villtype-tPA er endret til Asn, Asn117 fra human villtype-tPA er endret til Gln og Lys-His-Arg-Arg fra human villype-tPA er endret til Ala-Ala-Ala-Ala i posisjon 296-299 NO329876B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16360799P 1999-11-04 1999-11-04
PCT/US2000/030252 WO2001032915A2 (fr) 1999-11-04 2000-11-01 Methode de chromatographie liquide a haute performance en phase inverse pour activateurs de plasminogene

Publications (3)

Publication Number Publication Date
NO20022122D0 NO20022122D0 (no) 2002-05-03
NO20022122L NO20022122L (no) 2002-07-03
NO329876B1 true NO329876B1 (no) 2011-01-17

Family

ID=22590762

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20022122A NO329876B1 (no) 1999-11-04 2002-05-03 Fremgangsmate for overvaking av effektiviteten til en renseprosess ved fjerning av endogen plasminogenaktivator (PA) fra kinesisk hamsterovarie-(CHO)-celler, fra en prove som inneholder human tPA eller et tPA-molekyl hvori Thr103 fra human villtype-tPA er endret til Asn, Asn117 fra human villtype-tPA er endret til Gln og Lys-His-Arg-Arg fra human villype-tPA er endret til Ala-Ala-Ala-Ala i posisjon 296-299

Country Status (24)

Country Link
US (2) US6593097B1 (fr)
EP (1) EP1226269B1 (fr)
JP (1) JP4659320B2 (fr)
KR (1) KR100649043B1 (fr)
CN (1) CN1167808C (fr)
AT (1) ATE243263T1 (fr)
AU (1) AU781023B2 (fr)
BR (1) BRPI0015283B8 (fr)
CA (1) CA2384756C (fr)
CZ (1) CZ302023B6 (fr)
DE (1) DE60003449T2 (fr)
DK (1) DK1226269T3 (fr)
ES (1) ES2200973T3 (fr)
HK (1) HK1046158B (fr)
HU (1) HU226202B1 (fr)
IL (1) IL149006A0 (fr)
MX (1) MXPA02004401A (fr)
NO (1) NO329876B1 (fr)
NZ (1) NZ518187A (fr)
PL (1) PL204011B1 (fr)
PT (1) PT1226269E (fr)
SI (1) SI1226269T1 (fr)
WO (1) WO2001032915A2 (fr)
ZA (1) ZA200202687B (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2210948A3 (fr) 1999-12-10 2010-10-06 Life Technologies Corporation Utilisation de sites à recombinaison multiples avec une spécificité unique de clonage recombinatoire
AU2002227153B2 (en) 2000-12-08 2008-01-24 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6972327B1 (en) * 2001-05-08 2005-12-06 Immunex Corporation Regeneration of chromatography material
EP1885488B1 (fr) * 2005-05-24 2015-07-08 GE Healthcare Bio-Sciences AB Regeneration d'une matrice de chromatographie
KR100701265B1 (ko) * 2006-01-16 2007-04-02 대한민국 재조합 사람 조직형 플라스미노겐 활성인자 발현벡터 및 플라스미노겐 활성인자를 소변으로 분비하는 형질전환돼지 생산방법
JP5386354B2 (ja) * 2006-09-08 2014-01-15 ワイス・エルエルシー アフィニティークロマトグラフィーを使用するタンパク質精製におけるアルギニン洗浄
EP2089710B1 (fr) * 2006-10-27 2014-10-01 Janssen Pharmaceutica NV Procédé d'établissement de profils pharmacologiques de composés
CN101539550B (zh) * 2009-04-28 2012-05-23 李绍平 用于多糖的定性与定量分析方法
EP3592858B1 (fr) 2017-03-09 2024-06-12 Universität des Saarlandes Moyens et procédés pour la pyrolyse de lignine
CN108333281A (zh) * 2018-02-08 2018-07-27 吉林大学 一种测定重组人粒细胞集落刺激因子二硫键组成的方法
CN108918749B (zh) * 2018-07-17 2020-05-05 北京赛升药业股份有限公司 一种蛇毒纤溶酶的反相hplc检测方法
CN114426962B (zh) * 2021-12-21 2024-06-18 佛山汉腾生物科技有限公司 t-PA纯化方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI831484L (fi) 1982-05-05 1983-11-06 Genentech Inc Plasminogen aktivator foer maenskovaevnad
US4766075A (en) 1982-07-14 1988-08-23 Genentech, Inc. Human tissue plasminogen activator
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
US5120535A (en) * 1986-11-26 1992-06-09 Oncogen Oncostatin M and novel compositions having anti-neoplastic activity
US5411864A (en) * 1987-11-05 1995-05-02 Genentech, Inc. Method of purifying recombinant proteins from corresponding host cell proteins
GB8919803D0 (en) * 1989-09-01 1989-10-18 Ciba Geigy Pharmaceutical compositions
JP3559559B2 (ja) 1992-06-03 2004-09-02 ジェネンテク，インコーポレイテッド 向上した治療特性を有する組織プラスミノーゲン活性化因子グリコシル化変異体
US5753702A (en) * 1996-05-22 1998-05-19 University Of Vermont Arachidonic acid metabolite, 16-hete

Also Published As

Publication number Publication date
BRPI0015283B8 (pt) 2021-07-06
PT1226269E (pt) 2003-11-28
PL355389A1 (en) 2004-04-19
ZA200202687B (en) 2003-06-25
CZ302023B6 (cs) 2010-09-08
AU1581601A (en) 2001-05-14
HK1046158A1 (en) 2002-12-27
HUP0202973A3 (en) 2005-11-28
KR100649043B1 (ko) 2006-11-27
EP1226269B1 (fr) 2003-06-18
NO20022122D0 (no) 2002-05-03
AU781023B2 (en) 2005-04-28
BRPI0015283B1 (pt) 2015-03-17
PL204011B1 (pl) 2009-12-31
DE60003449T2 (de) 2004-05-06
IL149006A0 (en) 2002-11-10
ES2200973T3 (es) 2004-03-16
DE60003449D1 (de) 2003-07-24
BRPI0015283A (pt) 2002-07-02
JP2003513638A (ja) 2003-04-15
CN1387579A (zh) 2002-12-25
WO2001032915A3 (fr) 2002-03-07
MXPA02004401A (es) 2002-09-02
JP4659320B2 (ja) 2011-03-30
NO20022122L (no) 2002-07-03
HK1046158B (zh) 2003-11-28
CA2384756A1 (fr) 2001-05-10
HUP0202973A2 (hu) 2002-12-28
HU226202B1 (en) 2008-06-30
US6593097B1 (en) 2003-07-15
CN1167808C (zh) 2004-09-22
SI1226269T1 (en) 2003-12-31
EP1226269A2 (fr) 2002-07-31
CA2384756C (fr) 2010-09-28
US20030199016A1 (en) 2003-10-23
NZ518187A (en) 2002-12-20
WO2001032915A2 (fr) 2001-05-10
KR20020070431A (ko) 2002-09-09
DK1226269T3 (da) 2003-10-13
ATE243263T1 (de) 2003-07-15
US6828118B2 (en) 2004-12-07
CZ20021196A3 (cs) 2002-08-14

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