KR20020070431A - 플라스미노겐 활성화제에 대한 역상 고성능 액체크로마토그래피 분석법 - Google Patents
플라스미노겐 활성화제에 대한 역상 고성능 액체크로마토그래피 분석법 Download PDFInfo
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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Abstract
Description
Claims (11)
- 인간 tPA 또는 그의 변이체를 함유하는 시료를 인간 야생형 tPA의 Arg275-Ile276결합을 특이적으로 절단할 수 있는 프로테아제, 및 이어서 인간 야생형 tPA의 디술피드 결합을 환원시키기에 효과적인 양의 변성제 및 환원제와 함께 인큐베이션하고, 시료에 역상 고성능 액체 크로마토그래피 단계를 수행하고, 크로마토그래피 단계로부터의 용출 프로파일을 그에 존재하는 차이니스 햄스터 난소 (CHO) 세포에 내생성인 플라스미노겐 활성화제 (PA)의 양에 대해 분석하는 것을 포함하는, 상기 시료로부터 CHO 세포에 내생성인 PA의 제거에 있어서 정제 방법의 효과를 모니터하는 방법.
- 제1항에 있어서, 프로테아제가 플라스미노겐인 방법.
- 제1항에 있어서, 인간 tPA가 천연-서열의 tPA인 방법.
- 제1항에 있어서, tPA 변이체가 TNK-tPA인 방법.
- 제1항에 있어서, 인큐베이션 전에 시료를 분해 완충액으로 희석하는 방법.
- 제5항에 있어서, 완충액이 약 7 내지 8의 pH를 갖는 방법.
- 제6항에 있어서, 완충액이 인산염 완충액인 방법.
- 제7항에 있어서, 완충액이 아르기닌을 더 포함하는 방법.
- 제1항에 있어서, 변성제가 우레아 또는 구아니딘을 포함하는 방법.
- 제1항에 있어서, 환원제가 디티오트레이톨 또는 2-메르캅토에탄올을 포함하는 방법.
- 제1항에 있어서, 크로마토그래피 단계가 아세토니트릴을 포함하는 용매를 사용하여 구배 포맷으로 용출함으로써 수행되는 방법.
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US16360799P | 1999-11-04 | 1999-11-04 | |
US60/163,607 | 1999-11-04 |
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KR20020070431A true KR20020070431A (ko) | 2002-09-09 |
KR100649043B1 KR100649043B1 (ko) | 2006-11-27 |
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KR1020027005727A KR100649043B1 (ko) | 1999-11-04 | 2000-11-01 | 플라스미노겐 활성화제에 대한 역상 고성능 액체크로마토그래피 분석법 |
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US (2) | US6593097B1 (ko) |
EP (1) | EP1226269B1 (ko) |
JP (1) | JP4659320B2 (ko) |
KR (1) | KR100649043B1 (ko) |
CN (1) | CN1167808C (ko) |
AT (1) | ATE243263T1 (ko) |
AU (1) | AU781023B2 (ko) |
BR (1) | BRPI0015283B8 (ko) |
CA (1) | CA2384756C (ko) |
CZ (1) | CZ302023B6 (ko) |
DE (1) | DE60003449T2 (ko) |
DK (1) | DK1226269T3 (ko) |
ES (1) | ES2200973T3 (ko) |
HK (1) | HK1046158B (ko) |
HU (1) | HU226202B1 (ko) |
IL (1) | IL149006A0 (ko) |
MX (1) | MXPA02004401A (ko) |
NO (1) | NO329876B1 (ko) |
NZ (1) | NZ518187A (ko) |
PL (1) | PL204011B1 (ko) |
PT (1) | PT1226269E (ko) |
SI (1) | SI1226269T1 (ko) |
WO (1) | WO2001032915A2 (ko) |
ZA (1) | ZA200202687B (ko) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
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AU785483B2 (en) | 1999-12-10 | 2007-09-20 | Invitrogen Corporation | Use of multiple recombination sites with unique specificity in recombinational cloning |
EP2105496B1 (en) | 2000-12-08 | 2013-02-20 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6972327B1 (en) * | 2001-05-08 | 2005-12-06 | Immunex Corporation | Regeneration of chromatography material |
JP5148484B2 (ja) * | 2005-05-24 | 2013-02-20 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | クロマトグラフィーマトリックスの再生 |
KR100701265B1 (ko) * | 2006-01-16 | 2007-04-02 | 대한민국 | 재조합 사람 조직형 플라스미노겐 활성인자 발현벡터 및 플라스미노겐 활성인자를 소변으로 분비하는 형질전환돼지 생산방법 |
ES2764222T3 (es) * | 2006-09-08 | 2020-06-02 | Wyeth Llc | Lavado con arginina en la purificación de proteínas mediante el uso de cromatografía de afinidad |
EP2402752A3 (en) * | 2006-10-27 | 2012-06-06 | Janssen Pharmaceutica, N.V. | A method for pharmacologically profiling compounds |
CN101539550B (zh) * | 2009-04-28 | 2012-05-23 | 李绍平 | 用于多糖的定性与定量分析方法 |
EP3592858B1 (en) | 2017-03-09 | 2024-06-12 | Universität des Saarlandes | Means and methods for lignin pyrolysis |
CN108333281A (zh) * | 2018-02-08 | 2018-07-27 | 吉林大学 | 一种测定重组人粒细胞集落刺激因子二硫键组成的方法 |
CN108918749B (zh) * | 2018-07-17 | 2020-05-05 | 北京赛升药业股份有限公司 | 一种蛇毒纤溶酶的反相hplc检测方法 |
CN114426962B (zh) * | 2021-12-21 | 2024-06-18 | 佛山汉腾生物科技有限公司 | t-PA纯化方法 |
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FI831484L (fi) | 1982-05-05 | 1983-11-06 | Genentech Inc | Plasminogen aktivator foer maenskovaevnad |
US4766075A (en) | 1982-07-14 | 1988-08-23 | Genentech, Inc. | Human tissue plasminogen activator |
US4777043A (en) * | 1985-12-17 | 1988-10-11 | Genentech, Inc. | Stabilized human tissue plasminogen activator compositions |
US5120535A (en) * | 1986-11-26 | 1992-06-09 | Oncogen | Oncostatin M and novel compositions having anti-neoplastic activity |
US5411864A (en) * | 1987-11-05 | 1995-05-02 | Genentech, Inc. | Method of purifying recombinant proteins from corresponding host cell proteins |
GB8919803D0 (en) * | 1989-09-01 | 1989-10-18 | Ciba Geigy | Pharmaceutical compositions |
AU664469B2 (en) | 1992-06-03 | 1995-11-16 | Genentech Inc. | Tissue plasminogen activator glycosylation variants with improved therapeutic properties |
US5753702A (en) * | 1996-05-22 | 1998-05-19 | University Of Vermont | Arachidonic acid metabolite, 16-hete |
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