NO315379B1 - Isolert DNA-molekyl, hybridvektor, Aspergillus niger-stamme, Aspergillus niger-serin protease, fremgangsmåte for fremstilling av et önsketpolypeptid samtvert transformert med en hybrid ekspresjonvektor - Google Patents

Isolert DNA-molekyl, hybridvektor, Aspergillus niger-stamme, Aspergillus niger-serin protease, fremgangsmåte for fremstilling av et önsketpolypeptid samtvert transformert med en hybrid ekspresjonvektor Download PDF

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Publication number
NO315379B1
NO315379B1 NO19931368A NO931368A NO315379B1 NO 315379 B1 NO315379 B1 NO 315379B1 NO 19931368 A NO19931368 A NO 19931368A NO 931368 A NO931368 A NO 931368A NO 315379 B1 NO315379 B1 NO 315379B1
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NO
Norway
Prior art keywords
dna
aspergillus
ala
gene
ser
Prior art date
Application number
NO19931368A
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English (en)
Norwegian (no)
Other versions
NO931368L (no
NO931368D0 (no
Inventor
Frank Buxton
Albert Hinnen
Jacob Visser
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939305097A external-priority patent/GB9305097D0/en
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of NO931368D0 publication Critical patent/NO931368D0/no
Publication of NO931368L publication Critical patent/NO931368L/no
Publication of NO315379B1 publication Critical patent/NO315379B1/no

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
NO19931368A 1992-04-15 1993-04-14 Isolert DNA-molekyl, hybridvektor, Aspergillus niger-stamme, Aspergillus niger-serin protease, fremgangsmåte for fremstilling av et önsketpolypeptid samtvert transformert med en hybrid ekspresjonvektor NO315379B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP92810281 1992-04-15
GB939305097A GB9305097D0 (en) 1993-03-12 1993-03-12 Noval fungal protease

Publications (3)

Publication Number Publication Date
NO931368D0 NO931368D0 (no) 1993-04-14
NO931368L NO931368L (no) 1993-10-18
NO315379B1 true NO315379B1 (no) 2003-08-25

Family

ID=26132501

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19931368A NO315379B1 (no) 1992-04-15 1993-04-14 Isolert DNA-molekyl, hybridvektor, Aspergillus niger-stamme, Aspergillus niger-serin protease, fremgangsmåte for fremstilling av et önsketpolypeptid samtvert transformert med en hybrid ekspresjonvektor

Country Status (17)

Country Link
EP (1) EP0574347B1 (zh)
JP (2) JP3424840B2 (zh)
KR (1) KR100270644B1 (zh)
AT (1) ATE252156T1 (zh)
AU (1) AU663173B2 (zh)
CA (1) CA2093950C (zh)
CY (1) CY2507B1 (zh)
DE (1) DE69333249T2 (zh)
DK (1) DK0574347T3 (zh)
ES (1) ES2208637T3 (zh)
FI (1) FI112667B (zh)
HU (1) HU219809B (zh)
IL (2) IL170763A (zh)
MX (1) MX9302202A (zh)
NO (1) NO315379B1 (zh)
NZ (1) NZ247397A (zh)
PT (1) PT574347E (zh)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821350A (en) * 1995-11-01 1998-10-13 Nexia Biotechnologies, Inc. Aspergillus niger beta-galactosidase gene
EP0866872A1 (en) * 1995-12-15 1998-09-30 Novo Nordisk A/S A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED
WO1997035956A1 (en) * 1996-03-27 1997-10-02 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
JP2000511429A (ja) * 1996-06-05 2000-09-05 ギスト ブロカデス ベスローテン フェンノートシャップ 真菌メタロプロテアーゼ遺伝子
JPH1016992A (ja) * 1996-07-04 1998-01-20 Toagosei Co Ltd 接着剤用容器
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
ATE469223T1 (de) 2000-03-14 2010-06-15 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
ATE514770T1 (de) * 2005-10-17 2011-07-15 Novozymes As Verwendung von pilzmutanten zur expression von antikörpern
US8633010B2 (en) 2007-12-06 2014-01-21 Novozymes A/S Fungal PepC inhibitor
EP3000871A1 (en) 2009-12-18 2016-03-30 Novozymes, Inc. Methods for producing polypeptides in protease-deficient mutants of trichoderma
MD4186C1 (ro) * 2012-02-20 2013-06-30 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Tulpină de fungi Fusarium gibbosum - producătoare de proteaze acide şi neutre, xilanaze şi b-glucozidaze
CA2916905A1 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
WO2015025055A1 (en) * 2013-08-23 2015-02-26 Novozymes A/S Regulated pepc expression
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi

Also Published As

Publication number Publication date
IL170763A (en) 2007-10-31
NO931368L (no) 1993-10-18
EP0574347A3 (zh) 1994-04-13
FI931634A0 (fi) 1993-04-08
CA2093950A1 (en) 1993-10-16
IL105383A0 (en) 1993-08-18
AU3695993A (en) 1993-10-21
CA2093950C (en) 2006-05-09
ES2208637T3 (es) 2004-06-16
DE69333249T2 (de) 2004-07-29
HU219809B (hu) 2001-08-28
JPH0646863A (ja) 1994-02-22
NZ247397A (en) 1995-08-28
HUT67800A (en) 1995-05-29
MX9302202A (es) 1994-04-29
FI931634A (fi) 1993-10-16
AU663173B2 (en) 1995-09-28
JP3636455B2 (ja) 2005-04-06
FI112667B (fi) 2003-12-31
KR930021783A (ko) 1993-11-23
NO931368D0 (no) 1993-04-14
CY2507B1 (en) 2005-12-23
EP0574347B1 (en) 2003-10-15
JP3424840B2 (ja) 2003-07-07
DE69333249D1 (de) 2003-11-20
ATE252156T1 (de) 2003-11-15
DK0574347T3 (da) 2004-01-26
JP2003235591A (ja) 2003-08-26
EP0574347A2 (en) 1993-12-15
PT574347E (pt) 2004-02-27
KR100270644B1 (ko) 2000-11-01
HU9301087D0 (en) 1993-06-28

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