ATE514770T1 - Verwendung von pilzmutanten zur expression von antikörpern - Google Patents
Verwendung von pilzmutanten zur expression von antikörpernInfo
- Publication number
- ATE514770T1 ATE514770T1 AT06791474T AT06791474T ATE514770T1 AT E514770 T1 ATE514770 T1 AT E514770T1 AT 06791474 T AT06791474 T AT 06791474T AT 06791474 T AT06791474 T AT 06791474T AT E514770 T1 ATE514770 T1 AT E514770T1
- Authority
- AT
- Austria
- Prior art keywords
- endogenous
- activity
- protease activity
- kexin
- express antibodies
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200501452 | 2005-10-17 | ||
PCT/DK2006/000586 WO2007045248A1 (en) | 2005-10-17 | 2006-10-17 | Use of fungal mutants for expression of antibodies |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE514770T1 true ATE514770T1 (de) | 2011-07-15 |
Family
ID=37496608
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06791474T ATE514770T1 (de) | 2005-10-17 | 2006-10-17 | Verwendung von pilzmutanten zur expression von antikörpern |
Country Status (6)
Country | Link |
---|---|
US (1) | US7858360B2 (de) |
EP (1) | EP1941025B1 (de) |
CN (1) | CN101292024B (de) |
AT (1) | ATE514770T1 (de) |
DK (1) | DK1941025T3 (de) |
WO (1) | WO2007045248A1 (de) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005070962A1 (en) * | 2004-01-21 | 2005-08-04 | Novozymes A/S | Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell |
EP2201110A1 (de) | 2007-10-01 | 2010-06-30 | Novozymes A/S | Polypeptide mit protease-aktivität und dafür codierende polynukleotide |
WO2010059413A2 (en) | 2008-11-20 | 2010-05-27 | Novozymes, Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
JP5305353B2 (ja) * | 2009-06-23 | 2013-10-02 | ヤマサ醤油株式会社 | alpBをコードする遺伝子の機能が欠損している麹菌及びその利用 |
CN102762714B (zh) * | 2009-12-18 | 2015-11-25 | 诺维信股份有限公司 | 用于在木霉属的蛋白酶缺陷突变体中产生多肽的方法 |
CN103068978B (zh) | 2010-06-25 | 2017-01-18 | 诺维信公司 | 具有前导序列功能的多核苷酸 |
WO2011161207A1 (en) | 2010-06-25 | 2011-12-29 | Novozymes A/S | Polynucleotides having promoter activity |
EP2585601A1 (de) | 2010-06-25 | 2013-05-01 | Novozymes A/S | Polynukleotide mit promoterwirkung |
CN105420267A (zh) | 2010-11-30 | 2016-03-23 | 诺维信股份有限公司 | 用于在真菌细胞中表达基因的启动子 |
CN102174453A (zh) * | 2010-12-01 | 2011-09-07 | 江南大学 | 一种产碱性蛋白酶基因工程菌及其应用 |
WO2012078712A2 (en) | 2010-12-07 | 2012-06-14 | Vnomics Corp. | System and method for measuring and reducing vehicle fuel waste |
FI123425B (fi) * | 2011-03-31 | 2013-04-30 | Ab Enzymes Oy | Proteaasientsyymi ja sen käytöt |
EP2527448A1 (de) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze |
EP2527432A1 (de) | 2011-05-23 | 2012-11-28 | Novozymes A/S | Bidirektionaler Cytosin-Deaminase-codierender Selektionsmarker |
EP2721910A4 (de) * | 2011-06-15 | 2014-11-12 | Halliburton Energy Serv Inc | Brenner zur internen eindüsung grobkörniger hartmetallteilchen sowie zugehörige zusammensetzungen, systeme und verfahren |
WO2013048661A1 (en) | 2011-09-30 | 2013-04-04 | Codexis, Inc. | Fungal proteases |
AU2013207165B2 (en) | 2012-01-05 | 2018-10-04 | Glykos Finland Oy | Protease deficient filamentous fungal cells and methods of use thereof |
WO2013174927A1 (en) | 2012-05-23 | 2013-11-28 | Novartis International Pharmaceutical Limited | Production of fucosylated glycoproteins |
KR102111078B1 (ko) * | 2013-07-04 | 2020-05-18 | 노파르티스 아게 | O-만노실트랜스퍼라제 결핍 사상균 세포 및 이의 이용 방법 |
CN105980552A (zh) | 2013-07-10 | 2016-09-28 | 诺华股份有限公司 | 多种蛋白酶缺陷的丝状真菌细胞及其使用方法 |
CN103451173B (zh) * | 2013-08-21 | 2015-01-28 | 山东省农业科学院生物技术研究中心 | 通过单点突变缺失结构中的盐键提高pae酶活的方法 |
WO2015025055A1 (en) * | 2013-08-23 | 2015-02-26 | Novozymes A/S | Regulated pepc expression |
SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
US10430838B1 (en) | 2016-06-28 | 2019-10-01 | Snap Inc. | Methods and systems for generation, curation, and presentation of media collections with automated advertising |
WO2018167153A1 (en) | 2017-03-17 | 2018-09-20 | Novozymes A/S | Improved filamentous fungal host cell |
CN110724644A (zh) * | 2019-12-05 | 2020-01-24 | 西北农林科技大学 | 一种产重组人胰岛素原的里氏木霉工程菌及其应用 |
IL300173A (en) | 2020-07-31 | 2023-03-01 | Biotalys NV | expression host |
US20240156922A1 (en) * | 2021-03-25 | 2024-05-16 | Bio-Cat, Inc. | Fungal protease mixtures and uses thereof |
CN113151329B (zh) * | 2021-03-30 | 2023-09-08 | 云南师范大学 | 中性蛋白酶突变体及其构造方法和应用 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5190931A (en) | 1983-10-20 | 1993-03-02 | The Research Foundation Of State University Of New York | Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA |
US4885249A (en) | 1984-12-05 | 1989-12-05 | Allelix, Inc. | Aspergillus niger transformation system |
DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
CA1333777C (en) | 1988-07-01 | 1995-01-03 | Randy M. Berka | Aspartic proteinase deficient filamentous fungi |
ATE252156T1 (de) | 1992-04-15 | 2003-11-15 | Novartis Pharma Gmbh | Neue schimmel-protease |
EP1559776A3 (de) | 1994-06-30 | 2006-01-11 | Novozymes Biotech, Inc. | Nicht-toxisches, nicht-toxigenes, nicht-pathogenes Fusarium Expressionssystem und darin zu verwendende Promotoren und Terminatoren |
NL1000803C2 (nl) | 1995-07-14 | 1997-01-15 | Ambrosiushoeve Proefbedrijf | Werkwijze voor het telen van hommelkoninginnen en werkwijze voor het telen van hommels. |
JP2000501620A (ja) * | 1995-12-15 | 2000-02-15 | ノボ ノルディスク アクティーゼルスカブ | areA,pepC及び/又はpepE遺伝子が不活性化されている菌類 |
DK0894126T3 (da) | 1996-03-27 | 2006-06-12 | Novozymes As | Alkalisk phosphatase-deficient trådformet svamp |
JP4091119B2 (ja) | 1996-09-19 | 2008-05-28 | ノボザイムス アクティーゼルスカブ | 新規な宿主細胞およびタンパク質生産方法 |
ATE414782T1 (de) | 1998-12-23 | 2008-12-15 | Novozymes As | Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen |
JP4620253B2 (ja) | 1999-03-22 | 2011-01-26 | ノボザイムス,インコーポレイティド | 菌類細胞中で遺伝子を発現させるためのプロモーター |
JP2003516112A (ja) | 1999-05-06 | 2003-05-13 | アベンテイス・アニマル・ニユートリシヨン・エス・エー | 相同又は非相同タンパク質生産のための組換えペニシリウム フニクロスム |
CN1425068B (zh) | 2000-03-14 | 2011-05-18 | 诺维信公司 | 在制备多肽的方法中有效的真菌转录激活因子 |
US7063962B2 (en) | 2001-07-20 | 2006-06-20 | Novozymes A/S | DNA sequences for regulating transcription |
US20040018573A1 (en) * | 2002-04-18 | 2004-01-29 | Power Scott D | Production of functional antibodies in filamentous fungi |
-
2006
- 2006-10-17 EP EP06791474A patent/EP1941025B1/de active Active
- 2006-10-17 CN CN2006800387770A patent/CN101292024B/zh not_active Expired - Fee Related
- 2006-10-17 AT AT06791474T patent/ATE514770T1/de not_active IP Right Cessation
- 2006-10-17 WO PCT/DK2006/000586 patent/WO2007045248A1/en active Application Filing
- 2006-10-17 US US12/065,924 patent/US7858360B2/en active Active
- 2006-10-17 DK DK06791474.7T patent/DK1941025T3/da active
Also Published As
Publication number | Publication date |
---|---|
US7858360B2 (en) | 2010-12-28 |
DK1941025T3 (da) | 2011-09-26 |
WO2007045248A1 (en) | 2007-04-26 |
EP1941025B1 (de) | 2011-06-29 |
CN101292024A (zh) | 2008-10-22 |
US20080248530A1 (en) | 2008-10-09 |
EP1941025A1 (de) | 2008-07-09 |
CN101292024B (zh) | 2012-04-18 |
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Legal Events
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |