ATE514770T1 - Verwendung von pilzmutanten zur expression von antikörpern - Google Patents

Verwendung von pilzmutanten zur expression von antikörpern

Info

Publication number
ATE514770T1
ATE514770T1 AT06791474T AT06791474T ATE514770T1 AT E514770 T1 ATE514770 T1 AT E514770T1 AT 06791474 T AT06791474 T AT 06791474T AT 06791474 T AT06791474 T AT 06791474T AT E514770 T1 ATE514770 T1 AT E514770T1
Authority
AT
Austria
Prior art keywords
endogenous
activity
protease activity
kexin
express antibodies
Prior art date
Application number
AT06791474T
Other languages
English (en)
Inventor
Mogens Trier Hansen
Christian Isak Jyrgensen
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE514770T1 publication Critical patent/ATE514770T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT06791474T 2005-10-17 2006-10-17 Verwendung von pilzmutanten zur expression von antikörpern ATE514770T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200501452 2005-10-17
PCT/DK2006/000586 WO2007045248A1 (en) 2005-10-17 2006-10-17 Use of fungal mutants for expression of antibodies

Publications (1)

Publication Number Publication Date
ATE514770T1 true ATE514770T1 (de) 2011-07-15

Family

ID=37496608

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06791474T ATE514770T1 (de) 2005-10-17 2006-10-17 Verwendung von pilzmutanten zur expression von antikörpern

Country Status (6)

Country Link
US (1) US7858360B2 (de)
EP (1) EP1941025B1 (de)
CN (1) CN101292024B (de)
AT (1) ATE514770T1 (de)
DK (1) DK1941025T3 (de)
WO (1) WO2007045248A1 (de)

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WO2005070962A1 (en) * 2004-01-21 2005-08-04 Novozymes A/S Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell
US8383391B2 (en) 2007-10-01 2013-02-26 Novozymes A/S Mutant cell with deleted or disrupted genes encoding protease
WO2010059413A2 (en) 2008-11-20 2010-05-27 Novozymes, Inc. Polypeptides having amylolytic enhancing activity and polynucleotides encoding same
JP5305353B2 (ja) * 2009-06-23 2013-10-02 ヤマサ醤油株式会社 ａｌｐＢをコードする遺伝子の機能が欠損している麹菌及びその利用
DK2513290T3 (en) 2009-12-18 2016-01-25 Novozymes Inc Methods for Preparation of Polypeptides in Trichoderma Mutants with Protease Deficiency
WO2011161206A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having leader sequence function
CN103068988A (zh) 2010-06-25 2013-04-24 诺维信公司 具有启动子活性的多核苷酸
US8802412B2 (en) 2010-06-25 2014-08-12 Novozymes A/S Polynucleotides having promoter activity
CN103339259B (zh) 2010-11-30 2016-01-13 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子
CN102174453A (zh) * 2010-12-01 2011-09-07 江南大学 一种产碱性蛋白酶基因工程菌及其应用
MX2013006455A (es) 2010-12-07 2013-10-25 Vnomics Corp Sistema y metodo para medir y reducir el desperdicio de combustible del vehiculo.
FI123425B (fi) * 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
EP2527432A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Bidirektionaler Cytosin-Deaminase-codierender Selektionsmarker
EP2527448A1 (de) 2011-05-23 2012-11-28 Novozymes A/S Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze
US9358631B2 (en) * 2011-06-15 2016-06-07 Halliburton Energy Services, Inc. Coarse hard-metal particle internal injection torch and associated compositions, systems, and methods
EP2760997A4 (de) 2011-09-30 2015-02-11 Codexis Inc Pilzproteasen
EP2800809B1 (de) 2012-01-05 2018-03-07 Glykos Finland Oy Filamentöse pilzzellen mit proteasemangel und verwendungsverfahren dafür
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
MX364532B (es) * 2013-07-04 2019-04-29 Glykos Finland Oy Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.
KR20160035587A (ko) 2013-07-10 2016-03-31 노파르티스 아게 복수개의 프로테아제 결핍 사상형 진균 세포들 및 그의 이용방법
CN103451173B (zh) * 2013-08-21 2015-01-28 山东省农业科学院生物技术研究中心 通过单点突变缺失结构中的盐键提高pae酶活的方法
WO2015025055A1 (en) * 2013-08-23 2015-02-26 Novozymes A/S Regulated pepc expression
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
US10430838B1 (en) 2016-06-28 2019-10-01 Snap Inc. Methods and systems for generation, curation, and presentation of media collections with automated advertising
WO2018167153A1 (en) 2017-03-17 2018-09-20 Novozymes A/S Improved filamentous fungal host cell
CN110724644A (zh) * 2019-12-05 2020-01-24 西北农林科技大学 一种产重组人胰岛素原的里氏木霉工程菌及其应用
US20230265478A1 (en) 2020-07-31 2023-08-24 Biotalys NV Methods of increasing recombinant protein yields
WO2022204576A1 (en) * 2021-03-25 2022-09-29 Bio-Cat, Inc. Fungal protease mixtures and uses thereof
CN113151329B (zh) * 2021-03-30 2023-09-08 云南师范大学 中性蛋白酶突变体及其构造方法和应用

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Publication number Priority date Publication date Assignee Title
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
ES2208637T3 (es) * 1992-04-15 2004-06-16 Novartis Ag Nueva proteasa fungica.
EP0777737B1 (de) 1994-06-30 2005-05-04 Novozymes Biotech, Inc. Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
NL1000803C2 (nl) 1995-07-14 1997-01-15 Ambrosiushoeve Proefbedrijf Werkwijze voor het telen van hommelkoninginnen en werkwijze voor het telen van hommels.
WO1997022705A1 (en) * 1995-12-15 1997-06-26 Novo Nordisk A/S A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED
AU2152897A (en) 1996-03-27 1997-10-17 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
ATE313620T1 (de) 1996-09-19 2006-01-15 Novozymes As Wirtszellen und methoden für die produktion von proteinen
WO2000039322A1 (en) 1998-12-23 2000-07-06 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells
AU4025700A (en) 1999-03-22 2000-10-09 Novo Nordisk Biotech, Inc. Promoters for expressing genes in a fungal cell
JP2003516112A (ja) 1999-05-06 2003-05-13 アベンテイス・アニマル・ニユートリシヨン・エス・エー 相同又は非相同タンパク質生産のための組換えペニシリウム フニクロスム
DE60142226D1 (de) 2000-03-14 2010-07-08 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
US7063962B2 (en) 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
WO2003089614A2 (en) * 2002-04-18 2003-10-30 Genencor International, Inc. Production of functional antibodies in filamentous fungi

Also Published As

Publication number Publication date
US20080248530A1 (en) 2008-10-09
WO2007045248A1 (en) 2007-04-26
EP1941025A1 (de) 2008-07-09
US7858360B2 (en) 2010-12-28
EP1941025B1 (de) 2011-06-29
CN101292024B (zh) 2012-04-18
DK1941025T3 (da) 2011-09-26
CN101292024A (zh) 2008-10-22

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