EA200870619A1 - Модифицированный фермент дикамба-монооксигеназа и способы его применения - Google Patents

Модифицированный фермент дикамба-монооксигеназа и способы его применения

Info

Publication number
EA200870619A1
EA200870619A1 EA200870619A EA200870619A EA200870619A1 EA 200870619 A1 EA200870619 A1 EA 200870619A1 EA 200870619 A EA200870619 A EA 200870619A EA 200870619 A EA200870619 A EA 200870619A EA 200870619 A1 EA200870619 A1 EA 200870619A1
Authority
EA
Eurasian Patent Office
Prior art keywords
dicamba
methods
monoxygenase
application
dmo
Prior art date
Application number
EA200870619A
Other languages
English (en)
Other versions
EA017638B1 (ru
Inventor
Томас Е. Клементе
Разван Думитру
Пол К.К. Фэн
Станислав Фласинский
Дональд П. Викс
Original Assignee
Монсанто Текнолоджи Ллс
Борд Оф Риджентс Оф Дзе Юниверсити Оф Небраска
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38832672&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA200870619(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Монсанто Текнолоджи Ллс, Борд Оф Риджентс Оф Дзе Юниверсити Оф Небраска filed Critical Монсанто Текнолоджи Ллс
Publication of EA200870619A1 publication Critical patent/EA200870619A1/ru
Publication of EA017638B1 publication Critical patent/EA017638B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

В изобретении предлагается модифицированный вариант дикамба-монооксигеназы (DMO). Изобретение относится к неожиданному открытию того, что клетки, экспрессирующие указанный вариант DMO, демонстрируют высокий уровень толерантности к гербициду дикамба. Предлагаются композиции, включающие нуклеиновые кислоты, кодирующие DMO, и способы применения.
EA200870619A 2006-06-06 2007-06-06 Модифицированный полипептид с дикамба-монооксигеназной активностью, кодирующая его молекула нуклеиновой кислоты и способы их применения EA017638B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81115206P 2006-06-06 2006-06-06
US11/758,657 US7884262B2 (en) 2006-06-06 2007-06-05 Modified DMO enzyme and methods of its use
PCT/US2007/070514 WO2007146706A2 (en) 2006-06-06 2007-06-06 Modified dicamba monooxygenase enzyme and methods of its use

Publications (2)

Publication Number Publication Date
EA200870619A1 true EA200870619A1 (ru) 2009-06-30
EA017638B1 EA017638B1 (ru) 2013-02-28

Family

ID=38832672

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200870619A EA017638B1 (ru) 2006-06-06 2007-06-06 Модифицированный полипептид с дикамба-монооксигеназной активностью, кодирующая его молекула нуклеиновой кислоты и способы их применения

Country Status (19)

Country Link
US (1) US7884262B2 (ru)
EP (1) EP2032702B1 (ru)
KR (1) KR101421308B1 (ru)
AP (1) AP2812A (ru)
AT (1) ATE502109T1 (ru)
AU (1) AU2007257953B2 (ru)
BR (1) BRPI0712249B1 (ru)
CA (1) CA2653987C (ru)
CO (1) CO6140070A2 (ru)
DE (1) DE602007013222D1 (ru)
DK (1) DK2032702T3 (ru)
EA (1) EA017638B1 (ru)
GE (1) GEP20125504B (ru)
GT (1) GT200800274A (ru)
IL (1) IL195694A (ru)
MX (1) MX2008015741A (ru)
NZ (1) NZ573151A (ru)
WO (1) WO2007146706A2 (ru)
ZA (1) ZA200810237B (ru)

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