DK1941025T3 - Brug af svampemutanter til ekspression af antistoffer - Google Patents

Brug af svampemutanter til ekspression af antistoffer

Info

Publication number
DK1941025T3
DK1941025T3 DK06791474.7T DK06791474T DK1941025T3 DK 1941025 T3 DK1941025 T3 DK 1941025T3 DK 06791474 T DK06791474 T DK 06791474T DK 1941025 T3 DK1941025 T3 DK 1941025T3
Authority
DK
Denmark
Prior art keywords
endogenous
activity
protease activity
kexin
express antibodies
Prior art date
Application number
DK06791474.7T
Other languages
English (en)
Inventor
Christian Isak Joergensen
Jan Lehmbeck
Mogens Trier Hansen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1941025T3 publication Critical patent/DK1941025T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
DK06791474.7T 2005-10-17 2006-10-17 Brug af svampemutanter til ekspression af antistoffer DK1941025T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200501452 2005-10-17
PCT/DK2006/000586 WO2007045248A1 (en) 2005-10-17 2006-10-17 Use of fungal mutants for expression of antibodies

Publications (1)

Publication Number Publication Date
DK1941025T3 true DK1941025T3 (da) 2011-09-26

Family

ID=37496608

Family Applications (1)

Application Number Title Priority Date Filing Date
DK06791474.7T DK1941025T3 (da) 2005-10-17 2006-10-17 Brug af svampemutanter til ekspression af antistoffer

Country Status (6)

Country Link
US (1) US7858360B2 (da)
EP (1) EP1941025B1 (da)
CN (1) CN101292024B (da)
AT (1) ATE514770T1 (da)
DK (1) DK1941025T3 (da)
WO (1) WO2007045248A1 (da)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1711529A1 (en) * 2004-01-21 2006-10-18 Novozymes A/S Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell
JP2010539964A (ja) 2007-10-01 2010-12-24 ノボザイムス アクティーゼルスカブ プロテアーゼ活性を有するポリペプチド及びそれをコードするポリヌクレオチド
CN102292444A (zh) 2008-11-20 2011-12-21 诺维信股份有限公司 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸
JP5305353B2 (ja) * 2009-06-23 2013-10-02 ヤマサ醤油株式会社 ａｌｐＢをコードする遺伝子の機能が欠損している麹菌及びその利用
WO2011075677A2 (en) * 2009-12-18 2011-06-23 Novozymes, Inc. Methods for producing polypeptides in protease-deficient mutants of trichoderma
EP2585602A1 (en) 2010-06-25 2013-05-01 Novozymes A/S Polynucleotides having promoter activity
EP2585601A1 (en) 2010-06-25 2013-05-01 Novozymes A/S Polynucleotides having promoter activity
EP2585591B1 (en) 2010-06-25 2014-12-24 Novozymes A/S Polynucleotides having leader sequence function
EP2646558B1 (en) 2010-11-30 2016-07-20 Novozymes, Inc. Promoters for expressing genes in a fungal cell
CN102174453A (zh) * 2010-12-01 2011-09-07 江南大学 一种产碱性蛋白酶基因工程菌及其应用
CA2820248C (en) 2010-12-07 2019-10-29 Vnomics Corp. System and method for measuring and reducing vehicle fuel waste
FI123425B (fi) * 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
EP2527432A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Bi-directional cytosine deaminase-encoding selection marker
EP2721910A4 (en) * 2011-06-15 2014-11-12 Halliburton Energy Serv Inc BURNER FOR THE INTERNAL INJECTION OF ROASTED HARD-MOLDED PARTICLES AND RELATED COMPOSITIONS, SYSTEMS AND METHODS
US20130084608A1 (en) * 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
MX355226B (es) 2012-01-05 2018-03-23 Glykos Finland Oy Celulas de hongos filamentosas deficientes de proteasa y metodos de uso de las mismas.
EP2852610B1 (en) 2012-05-23 2018-07-11 Glykos Finland Oy Production of fucosylated glycoproteins
BR112015032695A2 (pt) * 2013-07-04 2017-10-24 Glykos Finland Oy células de fungos filamentosos deficientes em o-manosiltransferase e métodos de uso das mesmas
KR20160035587A (ko) 2013-07-10 2016-03-31 노파르티스 아게 복수개의 프로테아제 결핍 사상형 진균 세포들 및 그의 이용방법
CN103451173B (zh) * 2013-08-21 2015-01-28 山东省农业科学院生物技术研究中心 通过单点突变缺失结构中的盐键提高pae酶活的方法
EP3036324B1 (en) * 2013-08-23 2018-12-19 Novozymes A/S Regulated pepc expression
US10513724B2 (en) 2014-07-21 2019-12-24 Glykos Finland Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
US10430838B1 (en) 2016-06-28 2019-10-01 Snap Inc. Methods and systems for generation, curation, and presentation of media collections with automated advertising
WO2018167153A1 (en) 2017-03-17 2018-09-20 Novozymes A/S Improved filamentous fungal host cell
CN110724644A (zh) * 2019-12-05 2020-01-24 西北农林科技大学 一种产重组人胰岛素原的里氏木霉工程菌及其应用
EP4189060A1 (en) 2020-07-31 2023-06-07 Biotalys NV Expression host
CA3214495A1 (en) * 2021-03-25 2022-09-29 Bio-Cat, Inc. Fungal protease mixtures and uses thereof
CN113151329B (zh) * 2021-03-30 2023-09-08 云南师范大学 中性蛋白酶突变体及其构造方法和应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4885249A (en) 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
PT574347E (pt) 1992-04-15 2004-02-27 Novartis Ag Nova protease fungica
DE69534185T2 (de) 1994-06-30 2006-02-23 Novozymes Biotech, Inc., Davis Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
NL1000803C2 (nl) 1995-07-14 1997-01-15 Ambrosiushoeve Proefbedrijf Werkwijze voor het telen van hommelkoninginnen en werkwijze voor het telen van hommels.
EP0866872A1 (en) * 1995-12-15 1998-09-30 Novo Nordisk A/S A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED
JP4263241B2 (ja) 1996-03-27 2009-05-13 ノボザイムス アクティーゼルスカブ アルカリプロテアーゼを欠失した糸状真菌
DE69734936T2 (de) * 1996-09-19 2006-08-24 Novozymes A/S Wirtszellen und methoden für die produktion von proteinen
ES2317706T3 (es) 1998-12-23 2009-04-16 Novozymes A/S Metodo para produicir polipeptidos en celulas mutantes de aspergillus.
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
CA2372983A1 (en) 1999-05-06 2000-11-16 Aventis Animal Nutrition S.A. Recombinant penicillium funiculosum for homologous and heterologous protein production
AU2001242299A1 (en) 2000-03-14 2001-09-24 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
US7063962B2 (en) 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
US20040018573A1 (en) * 2002-04-18 2004-01-29 Power Scott D Production of functional antibodies in filamentous fungi

Also Published As

Publication number Publication date
CN101292024A (zh) 2008-10-22
US20080248530A1 (en) 2008-10-09
US7858360B2 (en) 2010-12-28
EP1941025B1 (en) 2011-06-29
ATE514770T1 (de) 2011-07-15
EP1941025A1 (en) 2008-07-09
CN101292024B (zh) 2012-04-18
WO2007045248A1 (en) 2007-04-26

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