MX9204926A - Clonacion molecular directa de un genoma de virus adn citoplasmico eucariotico. - Google Patents

Clonacion molecular directa de un genoma de virus adn citoplasmico eucariotico.

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Publication number
MX9204926A
MX9204926A MX9204926A MX9204926A MX9204926A MX 9204926 A MX9204926 A MX 9204926A MX 9204926 A MX9204926 A MX 9204926A MX 9204926 A MX9204926 A MX 9204926A MX 9204926 A MX9204926 A MX 9204926A
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Mexico
Prior art keywords
modified
genome
dna
dna molecule
poxvirus
Prior art date
Application number
MX9204926A
Other languages
English (en)
Inventor
Michael Pfleiderer
Friedrich Dorner
Friedrich Scheiflinger
Falko-Guenter Falkner
Original Assignee
Immuno Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/750,080 external-priority patent/US5445953A/en
Application filed by Immuno Ag filed Critical Immuno Ag
Publication of MX9204926A publication Critical patent/MX9204926A/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
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    • C12N9/14—Hydrolases (3)
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    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
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    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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    • C12Y304/21—Serine endopeptidases (3.4.21)
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    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24041—Use of virus, viral particle or viral elements as a vector
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    • C12N2710/00011—Details
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    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
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Abstract

Se describe un método para producir un virus ADN citoplásmico eucariótico por clonación molecular directo de una molécula de ADN modificada que comprende un genoma de virus ADN citoplásmico modificado. El método inventivo comprende las etapas de (I) modificar bajo condiciones extracelulares una molécula de ADN que incluye un genoma de un primer virus ADN citoplásmico, para producir una molécula de ADn modificada que comprende el genoma del virus ADN citoplásmico; (II) introducir la molécula de ADN modificada dentro de una primer célula huésped la cual empaca la molécula de ADN modificada dentro del virión infeccioso: y (III) recuperar de la célula huésped, los viriones constituídos por el genoma viral modificado. La célula huésped es infectada con un virus de ayuda el cual es expresado para empacar el genoma viral modificado dentro del virión infeccioso. Se describen ejemplos de empacamiento de un genoma de poxvirus modificado por un poxvirus de ayuda del mismo o diferente género. También se discuten vectores de poxvirus novedosos para la clonación molecular directa de estructuras de lectura abiertas dentro de un sitio de anclaje para enzima de restricción que es único en el vector. En un modelo de vector de poxvirus, la estructura de lectura abierta es transcrita por un promotor localizado en un vector de ADN aguas arriba de un sitio de clonación múltiple que comprende algunos sitios de anclaje únicos.
MX9204926A 1991-08-26 1992-08-26 Clonacion molecular directa de un genoma de virus adn citoplasmico eucariotico. MX9204926A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/750,080 US5445953A (en) 1991-08-26 1991-08-26 Direct molecular cloning of a modified poxvirus genome
US91473892A 1992-07-20 1992-07-20

Publications (1)

Publication Number Publication Date
MX9204926A true MX9204926A (es) 1994-02-28

Family

ID=27115227

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MX9204926A MX9204926A (es) 1991-08-26 1992-08-26 Clonacion molecular directa de un genoma de virus adn citoplasmico eucariotico.

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US (2) US6265183B1 (es)
EP (1) EP0561034B1 (es)
JP (1) JPH06261763A (es)
AT (1) ATE181108T1 (es)
AU (1) AU652467B2 (es)
BR (1) BR9203322A (es)
CA (1) CA2076839C (es)
CZ (1) CZ264192A3 (es)
DE (1) DE69229390T2 (es)
FI (1) FI111384B (es)
HU (1) HU219369B (es)
MX (1) MX9204926A (es)
NO (1) NO310306B1 (es)
PL (1) PL295733A1 (es)
SI (1) SI9200190A (es)
SK (1) SK264192A3 (es)
YU (1) YU79092A (es)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8927722D0 (en) * 1989-12-07 1990-02-07 British Bio Technology Proteins and nucleic acids
EP0753581A1 (en) 1995-07-10 1997-01-15 Immuno Ag Improved recombinant eukaryotic cytoplasmic viruses, method for their production and their use as vaccines
GB9711957D0 (en) * 1997-06-09 1997-08-06 Isis Innovation Methods and reagents for vaccination
US20030138454A1 (en) * 1997-06-09 2003-07-24 Oxxon Pharmaccines, Ltd. Vaccination method
CA2375189C (en) * 1999-05-28 2010-02-09 The Government Of The United States Of America A combined growth factor-deleted and thymidine kinase-deleted vaccinia virus vector
US20040063653A1 (en) * 2000-12-21 2004-04-01 Shiver John W. Polynucleotide vaccines expressing codon optimized hiv-1 pol and modified hiv-1 pol
US8623379B2 (en) * 2000-03-02 2014-01-07 Emory University Compositions and methods for generating an immune response
EP2388015A1 (en) 2000-03-02 2011-11-23 Emory University DNA expression vectors and methods of use
US20040105871A1 (en) * 2000-03-02 2004-06-03 Robinson Harriet L. Compositions and methods for generating an immune response
AU2002252199B2 (en) 2001-03-08 2008-01-03 Emory University MVA expressing modified HIV envelope, GAG, and POL genes
AU2002309335A1 (en) * 2001-05-23 2002-12-03 Fornix Biosciences N.V. Vectors for enhanced expression of vegf for disease treatment
US7223740B2 (en) 2001-05-23 2007-05-29 Fornix Biosciences N.V. Vectors for enhanced expression of VEGF for atrial disease treatment
GB0118532D0 (en) * 2001-07-30 2001-09-19 Isis Innovation Materials and methods relating to improved vaccination strategies
GB2382578A (en) * 2001-11-30 2003-06-04 Isis Innovation Fowlpox-based vaccine
GB2396814B (en) * 2001-11-30 2006-07-19 Isis Innovation Fowlpox vector
CA2425021A1 (en) * 2002-04-12 2003-10-12 Sumitomo Electric Industries, Ltd. Control of the ratio of lap to lip
CN102703393A (zh) * 2002-05-16 2012-10-03 巴法里安诺迪克有限公司 表达插入痘病毒基因组中的同源基因的重组痘病毒
GB0215621D0 (en) * 2002-07-05 2002-08-14 Imp College Innovations Ltd Improved poxvirus vaccines
US20050175627A1 (en) * 2003-09-24 2005-08-11 Oxxon Therapeutics Ltd. HIV pharmaccines
WO2008100292A2 (en) * 2006-10-16 2008-08-21 Genelux Corporation Modified vaccinia virus strains for use in diagnostic and therapeutic methods
US8202967B2 (en) 2006-10-27 2012-06-19 Boehringer Ingelheim Vetmedica, Inc. H5 proteins, nucleic acid molecules and vectors encoding for those, and their medicinal use
GB201006405D0 (en) 2010-04-16 2010-06-02 Isis Innovation Poxvirus expression system
AR083533A1 (es) 2010-10-22 2013-03-06 Boehringer Ingelheim Vetmed Proteinas de hemaglutinina 5 (h5) para el tratamiento y prevencion de las infecciones de gripe
CA2836299A1 (en) 2011-04-15 2012-10-18 Genelux Corporation Clonal strains of attenuated vaccinia viruses and methods of use thereof
AR088028A1 (es) 2011-08-15 2014-05-07 Boehringer Ingelheim Vetmed Proteinas h5, de h5n1 para un uso medicinal
WO2014055960A1 (en) 2012-10-05 2014-04-10 Genelux Corporation Energy absorbing-based diagnostic and therapeutic methods employing nucleic acid molecules encoding chromophore-producing enzymes
ES2689878T3 (es) 2013-02-21 2018-11-16 Boehringer Ingelheim Vetmedica Gmbh Proteínas H5 del virus de la gripe H5N1 para su uso como un medicamento
WO2015103438A2 (en) 2014-01-02 2015-07-09 Genelux Corporation Oncolytic virus adjunct therapy with agents that increase virus infectivity
BR102015012334A2 (pt) * 2015-05-27 2016-11-29 Fundação Hemoct De Ribeirão Preto Fundherp processo de produção do fator vii de coagulação sanguínea e fator vii de coagulação sanguínea
PL3402802T3 (pl) 2016-01-08 2023-06-05 Geovax, Inc. Kompozycje i sposoby generowania odpowiedzi immunologicznej względem antygenu powiązanego z guzem nowotworowym
US11311612B2 (en) 2017-09-19 2022-04-26 Geovax, Inc. Compositions and methods for generating an immune response to treat or prevent malaria
US20240033347A1 (en) 2022-07-08 2024-02-01 Viromissile, Inc. Oncolytic vaccinia viruses and recombinant viruses and methods of use thereof

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4603112A (en) 1981-12-24 1986-07-29 Health Research, Incorporated Modified vaccinia virus
US4722848A (en) 1982-12-08 1988-02-02 Health Research, Incorporated Method for immunizing animals with synthetically modified vaccinia virus
US5505941A (en) 1981-12-24 1996-04-09 Health Research, Inc. Recombinant avipox virus and method to induce an immune response
US5110587A (en) 1981-12-24 1992-05-05 Health Research, Incorporated Immunogenic composition comprising synthetically modified vaccinia virus
US5364773A (en) 1991-03-07 1994-11-15 Virogenetics Corporation Genetically engineered vaccine strain
US4769330A (en) 1981-12-24 1988-09-06 Health Research, Incorporated Modified vaccinia virus and methods for making and using the same
US5174993A (en) 1981-12-24 1992-12-29 Health Research Inc. Recombinant avipox virus and immunological use thereof
US5338683A (en) 1981-12-24 1994-08-16 Health Research Incorporated Vaccinia virus containing DNA sequences encoding herpesvirus glycoproteins
US5155020A (en) 1989-03-08 1992-10-13 Health Research Inc. Recombinant poxvirus host range selection system
US5135855A (en) * 1986-09-03 1992-08-04 The United States Of America As Represented By The Department Of Health And Human Services Rapid, versatile and simple system for expressing genes in eukaryotic cells
US5266313A (en) 1987-02-03 1993-11-30 The United States Of America As Represented By The Department Of Health And Human Services Raccoon poxvirus as a gene expression and vaccine vector for genes of rabies virus and other organisms
FR2619314B1 (fr) * 1987-08-11 1990-06-15 Transgene Sa Analogue du facteur viii, procede de preparation et composition pharmaceutique le contenant
NL9020389A (nl) 1989-03-08 1991-12-02 Health Research Inc Systeem voor het in de gastheer selecteren van recombinant-pokkenvirussen.
US5225336A (en) 1989-03-08 1993-07-06 Health Research Incorporated Recombinant poxvirus host range selection system
WO1991011519A1 (en) * 1990-01-26 1991-08-08 Immuno Aktiengesellschaft Recombinantly produced blood factors and process for the expression of said blood factors, as well as vaccinia virus recombinants used in said process
US5204243A (en) 1990-02-14 1993-04-20 Health Research Incorporated Recombinant poxvirus internal cores
KR100372934B1 (ko) 1990-09-25 2003-12-24 캔탑 파마슈티칼스 리서취 리미티드 형질 상보성 세포주에 의해 생성된 바이러스 결손 백신
JP3602530B2 (ja) 1991-03-07 2004-12-15 ヴァイロジェネティクス コーポレイション 遺伝子操作したワクチン菌株
US5997878A (en) 1991-03-07 1999-12-07 Connaught Laboratories Recombinant poxvirus-cytomegalovirus, compositions and uses
US5843456A (en) 1991-03-07 1998-12-01 Virogenetics Corporation Alvac poxvirus-rabies compositions and combination compositions and uses
US5863542A (en) 1991-03-07 1999-01-26 Virogenetics Corporation Recombinant attenuated ALVAC canaryopox virus containing heterologous HIV or SIV inserts
US5989561A (en) 1991-03-07 1999-11-23 Virogenetics Corporation Recombinant poxvirus-calicivirus rabbit hemorrhagic disease virus (RHDV) compositions and uses
DE69333751T2 (de) 1992-07-30 2005-12-29 Akzo Nobel N.V. Nicht-verbreitendes lebendes herpesvirusvakzin
CA2210294C (en) 1995-01-13 2003-11-11 Virogenetics Corporation Immunogenic compositions containing recombinant attenuated poxviruses expressing htlv antigens

Also Published As

Publication number Publication date
US6103244A (en) 2000-08-15
DE69229390T2 (de) 1999-11-11
FI923828A (fi) 1993-02-27
EP0561034B1 (en) 1999-06-09
CZ264192A3 (en) 1993-08-11
FI111384B (fi) 2003-07-15
HUT69927A (en) 1995-09-28
EP0561034A2 (en) 1993-09-22
CA2076839C (en) 2007-06-12
JPH06261763A (ja) 1994-09-20
HU219369B (en) 2001-03-28
AU2126992A (en) 1993-03-04
CA2076839A1 (en) 1993-02-27
US6265183B1 (en) 2001-07-24
SI9200190A (en) 1993-03-31
NO923323L (no) 1993-03-01
BR9203322A (pt) 1993-03-30
AU652467B2 (en) 1994-08-25
NO923323D0 (no) 1992-08-25
SK264192A3 (en) 1995-08-09
FI923828A0 (fi) 1992-08-26
NO310306B1 (no) 2001-06-18
PL295733A1 (en) 1993-05-17
EP0561034A3 (es) 1995-04-26
YU79092A (sh) 1995-12-04
ATE181108T1 (de) 1999-06-15
DE69229390D1 (de) 1999-07-15

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