CA2515166A1 - Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome - Google Patents

Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome Download PDF

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Publication number
CA2515166A1
CA2515166A1 CA002515166A CA2515166A CA2515166A1 CA 2515166 A1 CA2515166 A1 CA 2515166A1 CA 002515166 A CA002515166 A CA 002515166A CA 2515166 A CA2515166 A CA 2515166A CA 2515166 A1 CA2515166 A1 CA 2515166A1
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CA
Canada
Prior art keywords
modified
genome
dna molecule
poxvirus
molecular cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002515166A
Other languages
French (fr)
Other versions
CA2515166C (en
Inventor
Friedrich Dorner
Friedrich Scheiflinger
Falko Gunter Falkner
Michael Pfleiderer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter Healthcare SA
Original Assignee
Immuno Aktiengesellschaft
Friedrich Dorner
Friedrich Scheiflinger
Falko Gunter Falkner
Michael Pfleiderer
Baxter Aktiengesellschaft
Baxter Healthcare S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/750,080 external-priority patent/US5445953A/en
Application filed by Immuno Aktiengesellschaft, Friedrich Dorner, Friedrich Scheiflinger, Falko Gunter Falkner, Michael Pfleiderer, Baxter Aktiengesellschaft, Baxter Healthcare S.A. filed Critical Immuno Aktiengesellschaft
Priority to CA002558864A priority Critical patent/CA2558864C/en
Priority claimed from CA002076839A external-priority patent/CA2076839C/en
Publication of CA2515166A1 publication Critical patent/CA2515166A1/en
Application granted granted Critical
Publication of CA2515166C publication Critical patent/CA2515166C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.
CA002515166A 1991-08-26 1992-08-25 Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome Expired - Fee Related CA2515166C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002558864A CA2558864C (en) 1991-08-26 1992-08-25 Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US07/750,080 1991-08-26
US07/750,080 US5445953A (en) 1991-08-26 1991-08-26 Direct molecular cloning of a modified poxvirus genome
US91473892A 1992-07-20 1992-07-20
US07/914,738 1992-07-20
CA002076839A CA2076839C (en) 1991-08-26 1992-08-25 Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002076839A Division CA2076839C (en) 1991-08-26 1992-08-25 Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002558864A Division CA2558864C (en) 1991-08-26 1992-08-25 Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome

Publications (2)

Publication Number Publication Date
CA2515166A1 true CA2515166A1 (en) 1993-02-27
CA2515166C CA2515166C (en) 2007-05-15

Family

ID=35511213

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002515166A Expired - Fee Related CA2515166C (en) 1991-08-26 1992-08-25 Direct molecular cloning of a modified eukaryotic cytoplasmic dna virus genome

Country Status (1)

Country Link
CA (1) CA2515166C (en)

Also Published As

Publication number Publication date
CA2515166C (en) 2007-05-15

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