YU79092A - Direktno molekularno klonirnje modofikovanog eukariotičnog citoplazmatičnog dnk virusnog genoma - Google Patents

Direktno molekularno klonirnje modofikovanog eukariotičnog citoplazmatičnog dnk virusnog genoma

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Publication number
YU79092A
YU79092A YU79092A YU79092A YU79092A YU 79092 A YU79092 A YU 79092A YU 79092 A YU79092 A YU 79092A YU 79092 A YU79092 A YU 79092A YU 79092 A YU79092 A YU 79092A
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Yugoslavia
Prior art keywords
modified
dna
dna molecule
molecular cloning
viral genome
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Application number
YU79092A
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English (en)
Inventor
F. Dorner
F. Scheiflinger
F.G. Falkner
M. Pfleiderer
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Immuno Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from US07/750,080 external-priority patent/US5445953A/en
Application filed by Immuno Aktiengesellschaft filed Critical Immuno Aktiengesellschaft
Publication of YU79092A publication Critical patent/YU79092A/sh

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Abstract

otkriven je postupak za proizvodnju jednog modifikovanog eukariotičnog citoplazmatičnog DNK virusa direktnim molekularnim kloniranjem nekog modifikovanog DNK molekula koji uključuje jedan modifikovani citoplazmatični DNK virusni genom. Pronadjeni postupak uključuje stupnjeve: (I) modifikovanje pod vanćelijskim uslovima jednog DNK molekula koji uključuje jedan prvi citoplazmatični DNK virusni genom, da bi proizveo jedan modifiko van DNK molekul koji obuh-vata modifikovani citoplazmatični DNK virusni genom; (II) uvod-jenje modifikovanog DNK molekula u jednu prvu ćeliju domaćina, gde se pakuje modifikovani DNK molekul u infektivne virione; i (III) ponovno dobijanje iz ćelije domaćina, viriona koji su sastavljeni od modifikovanog virusnog genoma. Ćelija domaćina inficirana je sa jednim pomoćnim virusom koji je odredjen da pakuje modifikovane virusne genome u infektivne virone. Opisani su primeri pakovanja jednog modifikovanog genoma virusa izazivača boginja, pomoću nekog pomoćnog virusa izazivača boginja, istog ili različitog roda. Otkriveni su, takodje, novi vektori virusa izazivača boginja za direktno molekularno kloniranje otvorenih ok vira za čitanje, u jedno mesto raskidanja za neki restrikcioni enzim, koje je jedinstveno u vektoru. U jednom model - vektoru virusa izazivača boginja otvoreni okvir za čitanje transkribovan je pomoću jednog promotora lociranog u vektorskoj DNK uzvodno od jednog višestrukog mesta za kloniranje, koje se sastoji od nekoliko jedinstvenih mesta ras-kidanja.
YU79092A 1991-08-26 1992-08-25 Direktno molekularno klonirnje modofikovanog eukariotičnog citoplazmatičnog dnk virusnog genoma YU79092A (sh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/750,080 US5445953A (en) 1991-08-26 1991-08-26 Direct molecular cloning of a modified poxvirus genome
US91473892A 1992-07-20 1992-07-20

Publications (1)

Publication Number Publication Date
YU79092A true YU79092A (sh) 1995-12-04

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YU79092A YU79092A (sh) 1991-08-26 1992-08-25 Direktno molekularno klonirnje modofikovanog eukariotičnog citoplazmatičnog dnk virusnog genoma

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US (2) US6265183B1 (sh)
EP (1) EP0561034B1 (sh)
JP (1) JPH06261763A (sh)
AT (1) ATE181108T1 (sh)
AU (1) AU652467B2 (sh)
BR (1) BR9203322A (sh)
CA (1) CA2076839C (sh)
CZ (1) CZ264192A3 (sh)
DE (1) DE69229390T2 (sh)
FI (1) FI111384B (sh)
HU (1) HU219369B (sh)
MX (1) MX9204926A (sh)
NO (1) NO310306B1 (sh)
PL (1) PL295733A1 (sh)
SI (1) SI9200190A (sh)
SK (1) SK264192A3 (sh)
YU (1) YU79092A (sh)

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US5843456A (en) 1991-03-07 1998-12-01 Virogenetics Corporation Alvac poxvirus-rabies compositions and combination compositions and uses
AU4560693A (en) 1992-07-30 1994-03-03 Akzo Nobel N.V. Non-shedding live herpesvirus vaccine
EP0805854A4 (en) 1995-01-13 2000-06-07 Virogenetics Corp IMMUNOGENIC COMPOSITIONS WHICH RECOMBINANT CONTAINED WEAKENED POX VIRUSES THAT EXPECT AN HTLV ANTIQUE

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ATE181108T1 (de) 1999-06-15
NO923323D0 (no) 1992-08-25
EP0561034A2 (en) 1993-09-22
US6265183B1 (en) 2001-07-24
CZ264192A3 (en) 1993-08-11
FI923828A (fi) 1993-02-27
SI9200190A (en) 1993-03-31
SK264192A3 (en) 1995-08-09
DE69229390T2 (de) 1999-11-11
MX9204926A (es) 1994-02-28
NO310306B1 (no) 2001-06-18
JPH06261763A (ja) 1994-09-20
EP0561034A3 (sh) 1995-04-26
PL295733A1 (en) 1993-05-17
DE69229390D1 (de) 1999-07-15
NO923323L (no) 1993-03-01
AU2126992A (en) 1993-03-04
CA2076839A1 (en) 1993-02-27
EP0561034B1 (en) 1999-06-09
CA2076839C (en) 2007-06-12
AU652467B2 (en) 1994-08-25
US6103244A (en) 2000-08-15
BR9203322A (pt) 1993-03-30
FI923828A0 (fi) 1992-08-26
HUT69927A (en) 1995-09-28
HU219369B (en) 2001-03-28
FI111384B (fi) 2003-07-15

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