AR020076A1 - LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA. - Google Patents

LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA.

Info

Publication number
AR020076A1
AR020076A1 ARP990102364A ARP990102364A AR020076A1 AR 020076 A1 AR020076 A1 AR 020076A1 AR P990102364 A ARP990102364 A AR P990102364A AR P990102364 A ARP990102364 A AR P990102364A AR 020076 A1 AR020076 A1 AR 020076A1
Authority
AR
Argentina
Prior art keywords
production
cell line
genes
retroviral vector
packaging cell
Prior art date
Application number
ARP990102364A
Other languages
English (en)
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of AR020076A1 publication Critical patent/AR020076A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Abstract

Línea celular de empaquetamiento retroviral anfotropico que contiene genes funcionales gag, pol y env integrados en el genoma, donde la expresion de losgenes gag y pol está regulada independientemente de la expresion de los genes env, donde elgenoma de dicha línea celular contiene uno o dos genes envfuncionalmente activos para la obtencion de una titulacion de vectores retrovirales de al menos 107 de unidades que forman colonias /ml de sobrenadantes decultivo celular; proceso para suproduccion , virus vectorial retroviral incompetente a la replicacion y proceso para su produccion.
ARP990102364A 1998-05-20 1999-05-18 LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA. AR020076A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP98109135 1998-05-20

Publications (1)

Publication Number Publication Date
AR020076A1 true AR020076A1 (es) 2002-04-10

Family

ID=8231962

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990102364A AR020076A1 (es) 1998-05-20 1999-05-18 LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA.

Country Status (13)

Country Link
US (1) US6475786B1 (es)
EP (1) EP1080219B1 (es)
JP (1) JP4571307B2 (es)
KR (1) KR100653154B1 (es)
CN (1) CN1154743C (es)
AR (1) AR020076A1 (es)
AT (1) ATE378416T1 (es)
AU (1) AU4261599A (es)
CA (1) CA2331440C (es)
DE (1) DE69937556T2 (es)
DK (1) DK1080219T3 (es)
ES (1) ES2296410T3 (es)
WO (1) WO1999060143A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2425001A4 (en) 2009-04-30 2012-11-14 Univ California ANTI-HIV COMBINATION VECTORS, TARGETING VECTORS AND METHOD OF USE
WO2014026053A1 (en) 2012-08-09 2014-02-13 The Regents Of The University Of California Cd25 pre-selective combination anti-hiv vectors, targeting vectors, and methods of use
US20140065110A1 (en) 2012-08-31 2014-03-06 The Regents Of The University Of California Genetically modified msc and therapeutic methods
US11912986B2 (en) 2019-09-23 2024-02-27 The Regents Of The University Of California Methods for screening genetic perturbations

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989007150A1 (en) 1988-02-05 1989-08-10 The Trustees Of Columbia University In The City Of Retroviral packaging cell lines and processes of using same
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines

Also Published As

Publication number Publication date
AU4261599A (en) 1999-12-06
DE69937556D1 (de) 2007-12-27
DE69937556T2 (de) 2008-10-23
CN1154743C (zh) 2004-06-23
JP2002515255A (ja) 2002-05-28
EP1080219B1 (en) 2007-11-14
CA2331440A1 (en) 1999-11-25
KR20010071300A (ko) 2001-07-28
CA2331440C (en) 2010-07-13
ATE378416T1 (de) 2007-11-15
KR100653154B1 (ko) 2006-12-01
DK1080219T3 (da) 2008-03-03
WO1999060143A1 (en) 1999-11-25
JP4571307B2 (ja) 2010-10-27
ES2296410T3 (es) 2008-04-16
EP1080219A1 (en) 2001-03-07
US6475786B1 (en) 2002-11-05
CN1300325A (zh) 2001-06-20

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