KR830005354A - 인간의 섬유 아세포 인터페론과 같은 폴리펩타이드의 제조방법, 재결합 dna 분자 및 dna 서열 - Google Patents
인간의 섬유 아세포 인터페론과 같은 폴리펩타이드의 제조방법, 재결합 dna 분자 및 dna 서열 Download PDFInfo
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Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 재결합 DNA 분자를 제조하는 대략의 공정도임.
제2도는 초기 클로운 분리 공정도임.
제3도는 DNA 서열을 사용한 클로운 분리 공정도임.
제4도는 HuIFN-β DNA의 암호섬유의 조성 뉴클 레오티드 서열임.
제5도는 몇 가지 플라스미드의 방향 및 억졔지도임.
제8도는 본 발명의 재결합 DNA분자 pPLa-HFIF-67-1의 구축도임.
Claims (31)
- G-pBR322(Pst)/HFIF 1, G-pBR322(Pst)/HFIF 3, G-pBR322(Pst)/HFIF 6, G-pBR322(Pst)/HFIF 6, G-pBR322(Pst)/HFIF 7의 DNA삽입물, 위 DNA 삽입물 중 어느것에 혼성화하는 DNA 서열 및 그 재료가 천연, 합성 혹은 반합성중 어느것일지라도 전술한 DNA서열 혹은 삽입물 중 어느 것을 단일 도는 다중 염기치환, 삭제, 삽입 및 전화의돌연변이로 관계된 DNA 서열로 구성되는 그룹으로부터 선택되는데에 특징이 있는 DNA 서열.
- G-pPLa-HFIF-67-12, G-pPLa-HFIF-67-12-19, G-pPLc-HFIF-67-8의 DNA 삽입물, 전술한 DNA 삽입물 중 어느것에 혼성하는 DNA 서열, 그 재료가 천연, 합성, 혹은 반합성중 어느것일지라도 전술한 DNA서열 혹은 삽임물 중 어느 것을 단일 또는 다중 염기 치환, 삭제, 삽입 및 전화의 돌연변이로 관계된 DNA서열, 및 전술한 DNA서열 및 삽입물 중 어느것의 코돈의 표현에 대한 암호가 된 폴리펩타이드와 동일한 면역학 혹은 생물학적 활성을 보이는 폴리펩타이드에 대한 암호를 표현시 지시하는 코돈의 서열로 구성되는 DNA서열로 구성되는 그룹으로부터 상기의 에혼성하는 상기의 DNA 서열이 선택되는데에 특징이 있는 청구범위 1에 따른 DNA서열.
- G-pPLa-HFIF-67-12 △279T, G-pPLa-HFIF-67-12 △218M1, G-pPLa-HFIF-67-12 M1, G-pPLa-HFIF-67-12 △19 BX-2, 전술한 DNA 서열에 혼성하는 DNA 서열, 그 재료가 천연, 합성, 혹은 반합성 재료중 어느것에서 나왔을지라도 전술한 DNA 서열중 어느것에 단일 또는 다중의 염기치환, 삭제, 삽입 및 전화의 돌연변이로 관계된 DNA 서열 및 전술한 DNA 서열중 어느것의 표현에 대한 암호가 된 폴리펩타이드와 면역학 또는 생물학적 활성이 동일한 폴리펩타이드에 대한 표현시 암호가 되는 코돈의 서열로 구성되는 DNA 서열로 구성되는 그룹으로부터 상기의 DNA 서열에 혼성하는 상기 DNA 서열이 선택되는데에 특징이 있는 청구범위 1 또는 2에 따르는 DNA 서열.
- 상기 DNA 서열이 혼성하는 상기 DNA 서열이 p[325]-gHFIF-4의 DNA 삽입물, 상기 DNA 서열에 혼성하는 DNA 서열, 그 재료가 천연, 합성 또는 반합성중 어느것일지라도 상기 DNA 서열에 단일 또는 다중의 염기치환, 삭제 삽입 및 전화의 돌연변이로 관계된 DNA 서열 및 상기 DNA 서열의 표현에 대하여 암호를 지시하는 폴리펩타이드와 면역학 혹은 생물학적 활성이 동일한 폴리펩타이드에 대한 암호를 표현시 지시하는 코돈의 서열로 구성되는 DNA 서열인데에 특징이 있는 청구범위 1에서 3중 어느것에 따르는 DNA 서열.
- 다음 구조의 DNA서열 :및 이것의 유도체, β인터페론의 면역학 또는 생물학적 활성을 보이는 폴리펩타이드에 대한 암호가 되는 유도체 및 그 부분으로 구성되는 그룹으로부터 선택되는데에 있는 청구범위 2에서 4중 어느 것에 따르는 DNA 서열.
- 전술한 청구범위들 중 어느 것에 따르는 DNA 서열로 특징지어진 재결합 DNA분자.
- 상기 DNA 서열이 욋과적으로 표현조정서열에 결합하는 데에 특징이 있는 청구범위 6에 따르는 재결합 DNA 분자.
- 상기 표현 조정서열이 lac 시스템, β-lac 시스템, trp 시스템, 주작동자 및 파지 λ의 촉진자 지역, fd막 단백질의 콘트롤 지역 및 원핵 혹은 진핵세포 및 그 비루스 유전자 표현을 통제하는 서열로 구성되는 그룹으로부터 선택되는데에 특징이 있는 청구범위 7에 따르는 재결합 DNA 분자.
- G-pPLa-HFIF-67-12, G-pPLa-HFIF-67-12 Δ19 혹은 G-pPLa-HFIF-67-8로 구성되는데에 특징이 있는 청구범위 7 또는 8에 따르는 재결합 DNA 분자.
- G-pPLa-HFIF-67-12 Δ279T, 6-pPLa-HFIF-67-12 Δ218M, 1,6-pPLa-HFIF-67-12M1, 혹은 G-pPLa-HFIF-67-12 Δ19 BX-2로 구성되는데에 특징이 있는 청구범위 7 또는 8에 따르는 재결합 DNA 분자.
- 청구범위 6에서 10에 따르는 재결합 DNA 분자로 변리된 숙주.
- 대장균, 슈도모나스, 고초균, 바실러스 스테아로터 모피러스, 다른 간균, 효모 진균류, 생쥐 혹은 다른 동식물 숙주 및 인간 조직 세포로 구성되는 그룹으로부터 선택되는데에 특징이 있는 청구범위 11의 변이된 숙주.
- 대장균 HB101(G-pBR 322(Pst)/HFIFI), 대장균 HB101(G-pBR 322(Pst)/HFIF3) 대장균 HB101(G-pBR 322(Pst)/HFIF6) 혹은 대장균 HB101(G-pBR 322(Pst)/HFIF7)으로 구성되는데에 특징이 있는 청구범위 11 또는 12에 따르는 변이된 숙주.
- 대장균 M5219(G-pPLa-HFIF-67-12), 대장균 K12HI(G-pPLa-HFIF-67-12), 대장균 M5219(G-pPLa-HFIF-67-1219), 대장균 M5219(G-pPLa-HFIF-67-8) 혹은 대장균 K12ΔHI(G-pPLa-HFIF-67-8)로 구성되는 청구범위 11 혹은 12에 따르는 변리된 숙주.
- 대장균 M5219(G-pPLa-HFIF-67-12Δ279T), 대장균 M5219(pPLa-HFIF-67-12Δ218M1) 대장균 M5219(pPLa-HFIF-67-12ΔM1)혹은 대장균 K12HI(pPLa-HFIF-67-1219 BX-2)로 구성되는 청구범위 11과 12에 따르는 변이된 숙주.
- 청구범위 11에서 15중 어느것에 따르는 변이된 숙주로 생상된 인간섬유 아세포 인터페론의 면역학 혹은 생물학적 활성을 보이는데에 특징이 있는 폴리펩타이드 혹은 부분 및 그것의 유도체.
- 청구범위 1에서 5중 어느 것에 따르는 DAN 서열로 암호가 주어진 데에 특징이 있는 폴리펩타이드.
- 인터페론으로 구성되는 데에 특징이 있는 청구범위 16 혹은 17에 따르는 폴리펩타이드 혹은 부분 및 그것의 유도체.
- 다음 구조식의 폴리펩타이드 :및 그 재료가 이것에 대한 암호를 지시하는 DNA 서열 중어느것을 단일 혹은 다중의 염기치환, 삭제, 삽입 및 전화를 포함한 돌연변이로 전술한 폴리펩타이드에연관된 폴리펩타이드로 구성되는 그룹으로부터 선택되는데에 특징이 있는 청구범위 16에서 18중 어느것에따르는 폴리펩타이드 혹은 부분 및 이것의 유도체.
- 클로닝 매개물에 청구범위 1-5중 어느 것에 따르는 서열을 삽입하는데에 특징이 있는 재결합 DNA 분자를 생산하는 방법.
- 상기 DNA 서열의 표현을 통제 및 억제하도록 상기 표현 조정시열이 상기 클로닝 매개물에 삽입되도록 하는 상기의 클로닝 매개물에 청구범위 8에 따르는 표현 콘트롤 서열을 삽입하는 것에 특징이 있는 청구범위 20에 따르는 방법.
- 청구범위 6에서 10중 어느것에 따르는 숙주에 재결합 DNA 분자를 삽입하는데에 특징이 있는 숙주를 변이시키는 방법.
- 알맞는 숙주를 청구범위 8에서 10중 어느것에 따라서 재결합 DNA 분자로 변이시키고; 상기 숙주를 배양하고; 상기 폴리펩타이드를 수집하는 데에 특징이 있는 인간 섬유 아세포 인터페론의 면역학 또는 생물학적 활성을 보이는 폴리펩타이드 제조방법.
- 대장균, 슈도모나스, 고초균, 바실러스 스테아로터모필러스, 다른 간균, 효모, 진균, 동식물 숙주 혹은 인간조직세포의 종으로 숙주가 구성되는데에 특징이 있는 청구범위 23에 따르는 방법.
- 청구범위 8에서 10중 어느 것에 따르는 재결합 DNA 분자로 변이된 숙주를 배양하고 상기 폴리펩타이드를 수집하여 변이된 숙주를 배양하는데에 특징이 있는 인간섬유 아세포 인터페론 면역학 또는 생물학적 활성을 보이는 폴리펩타이드에 대한 암호가 되는 DNA 서열을 제조하는 방법.
- 상기 DNA 서열중 어느것이 청구범위 1-5중 어느것에 따르는 DNA 서열에 혼성하는가를 결정하는데에 특징이 있는 ㅍ 서열의 그룹으로부터 인간 인터페론의 면역학 또는 생물학적 환성을 보이는 폴리펩타이드에 대한 암호를 지시하는 DNA 서열ㅇ르 선택하는 공정.
- 천연재료의 DNA서열, 합성 DNA 서열, 재결합 DNA 분자로부터의 DNA 서열 혹은 전술한 DNA 서열중 어느 것의 혼합인 서열로 상기 DNA 서열이 구성되는데에 특징이 있는 청구범위 26의 공정.
- 청구범위 16에서 18중 어느것에 따라서, 폴리펩타이드로 구성되는 그룹으로부터 선택된 최소한 한가지 폴립펩타이드로 특징이 주어진 인간의 암 혹은 육종 또는 비루스 질병을 치료하는 조성물.
- 청구범위 16에서 18중 어느것에 따라서, 폴리펩타이드로 구성되는 그룹으로부터 선택된 최소한 한가지 폴리펩타이드로 주어진 소비루스 질병을 치료하는 조성물.
- 환자에게제 약학적으로 알맞는 방법에 의하여 청구범위 28에 따르는 효과적인 양의 조성물을 가하는 인간의 암, 육종 비루스 질병을 치료하는 방법.
- 환자에게 제약학적으로 알맞는 방법에 의하여 청구범위 29에 따르는 효과적인 양의 조성물을 가하는 소비루스 질병을 치료하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB8011306 | 1980-04-03 | ||
| GB11306 | 1980-04-03 | ||
| GB8018701 | 1980-06-06 | ||
| GB80.18701 | 1980-06-06 |
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| Publication Number | Publication Date |
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| KR830005354A true KR830005354A (ko) | 1983-08-13 |
| KR860001471B1 KR860001471B1 (ko) | 1986-09-26 |
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| KR1019810001103A KR860001471B1 (ko) | 1980-04-03 | 1981-04-01 | 인체의 섬유아세포 인터페론(IFN-β)폴리펩티드의 제조방법 |
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| JP (1) | JP2687995B2 (ko) |
| KR (1) | KR860001471B1 (ko) |
| AT (1) | ATE56471T1 (ko) |
| AU (1) | AU564690B2 (ko) |
| BG (1) | BG60441B2 (ko) |
| CA (1) | CA1341604C (ko) |
| DD (1) | DD160280A5 (ko) |
| DE (1) | DE3177213D1 (ko) |
| DK (1) | DK170476B1 (ko) |
| ES (1) | ES500966A0 (ko) |
| FI (1) | FI88175C (ko) |
| GR (1) | GR74498B (ko) |
| HK (1) | HK160095A (ko) |
| HU (1) | HU193507B (ko) |
| IE (1) | IE57069B1 (ko) |
| IL (1) | IL62552A0 (ko) |
| IN (1) | IN156128B (ko) |
| LU (1) | LU90131I2 (ko) |
| MX (1) | MX9202946A (ko) |
| NL (2) | NL970018I1 (ko) |
| NO (3) | NO811118L (ko) |
| NZ (1) | NZ196706A (ko) |
| PL (1) | PL149079B1 (ko) |
| PT (1) | PT72786B (ko) |
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| NZ210501A (en) | 1983-12-13 | 1991-08-27 | Kirin Amgen Inc | Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence |
| AU594014B2 (en) | 1984-03-21 | 1990-03-01 | Research Corporation Technologies, Inc. | Recombinant DNA molecules |
| NZ212207A (en) | 1984-05-31 | 1991-07-26 | Genentech Inc | Recombinant lymphotoxin |
| EP0200425B1 (en) | 1985-04-22 | 1991-12-27 | Eli Lilly And Company | Recombinant dna expression vectors and dna compounds |
| US4892819A (en) | 1985-11-25 | 1990-01-09 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum |
| EP0257994B1 (en) | 1986-08-29 | 1992-09-16 | Tonen Corporation | Dna coding for rinderpest virus antigen, its preparation and use |
| DE3642096A1 (de) | 1986-12-10 | 1988-06-16 | Boehringer Ingelheim Int | Pferde-(gamma)-interferon |
| ES2052630T3 (es) | 1987-04-09 | 1994-07-16 | Shionogi & Co | Gen reg y proteina codificada por el gen. |
| ZA887773B (en) | 1987-10-26 | 1989-07-26 | Immunex Corp | Interleukin-7 |
| US5258287A (en) | 1988-03-22 | 1993-11-02 | Genentech, Inc. | DNA encoding and methods of production of insulin-like growth factor binding protein BP53 |
-
1981
- 1981-04-01 EP EP81301414A patent/EP0041313B1/en not_active Revoked
- 1981-04-01 YU YU859/81A patent/YU45104B/xx unknown
- 1981-04-01 CA CA374378A patent/CA1341604C/en active Active
- 1981-04-01 PT PT72786A patent/PT72786B/pt unknown
- 1981-04-01 IL IL62552A patent/IL62552A0/xx unknown
- 1981-04-01 AT AT81301414T patent/ATE56471T1/de active
- 1981-04-01 IE IE756/81A patent/IE57069B1/en not_active IP Right Cessation
- 1981-04-01 ES ES500966A patent/ES500966A0/es active Granted
- 1981-04-01 AU AU68979/81A patent/AU564690B2/en not_active Expired
- 1981-04-01 DK DK148581A patent/DK170476B1/da not_active IP Right Cessation
- 1981-04-01 NO NO811118A patent/NO811118L/no unknown
- 1981-04-01 NZ NZ196706A patent/NZ196706A/en unknown
- 1981-04-01 FI FI811008A patent/FI88175C/fi not_active IP Right Cessation
- 1981-04-01 HU HU81841A patent/HU193507B/hu unknown
- 1981-04-01 IN IN188/DEL/81A patent/IN156128B/en unknown
- 1981-04-01 DE DE8181301414T patent/DE3177213D1/de not_active Revoked
- 1981-04-01 JP JP56047361A patent/JP2687995B2/ja not_active Expired - Lifetime
- 1981-04-01 GR GR64561A patent/GR74498B/el unknown
- 1981-04-01 KR KR1019810001103A patent/KR860001471B1/ko active
- 1981-04-02 DD DD81228897A patent/DD160280A5/de active IP Right Maintenance
- 1981-04-02 PL PL1981230484A patent/PL149079B1/pl unknown
-
1985
- 1985-02-22 NO NO850735A patent/NO850735L/no unknown
- 1985-02-22 NO NO850734A patent/NO850734L/no unknown
-
1992
- 1992-06-17 MX MX9202946A patent/MX9202946A/es unknown
-
1994
- 1994-02-11 BG BG98456A patent/BG60441B2/bg unknown
-
1995
- 1995-05-25 US US08/452,658 patent/US7635466B1/en active Active
- 1995-05-25 US US08/449,930 patent/US7588755B1/en active Active
- 1995-10-12 HK HK160095A patent/HK160095A/xx not_active IP Right Cessation
-
1997
- 1997-04-07 NL NL970018C patent/NL970018I1/nl unknown
- 1997-08-13 NL NL970032C patent/NL970032I2/nl unknown
- 1997-09-03 LU LU90131C patent/LU90131I2/fr unknown
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