KR20160034901A - 서열 조작에 최적화된 crispr-cas 이중 닉카아제 시스템, 방법 및 조성물 - Google Patents
서열 조작에 최적화된 crispr-cas 이중 닉카아제 시스템, 방법 및 조성물 Download PDFInfo
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- KR20160034901A KR20160034901A KR1020167001293A KR20167001293A KR20160034901A KR 20160034901 A KR20160034901 A KR 20160034901A KR 1020167001293 A KR1020167001293 A KR 1020167001293A KR 20167001293 A KR20167001293 A KR 20167001293A KR 20160034901 A KR20160034901 A KR 20160034901A
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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Applications Claiming Priority (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361836123P | 2013-06-17 | 2013-06-17 | |
| US61/836,123 | 2013-06-17 | ||
| US201361847537P | 2013-07-17 | 2013-07-17 | |
| US61/847,537 | 2013-07-17 | ||
| US201361862355P | 2013-08-05 | 2013-08-05 | |
| US61/862,355 | 2013-08-05 | ||
| US201361871301P | 2013-08-28 | 2013-08-28 | |
| US61/871,301 | 2013-08-28 | ||
| US201361915383P | 2013-12-12 | 2013-12-12 | |
| US61/915,383 | 2013-12-12 | ||
| USPCT/US2013/074667 | 2013-12-12 | ||
| PCT/US2013/074667 WO2014093622A2 (en) | 2012-12-12 | 2013-12-12 | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| PCT/US2014/041803 WO2014204725A1 (en) | 2013-06-17 | 2014-06-10 | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20160034901A true KR20160034901A (ko) | 2016-03-30 |
Family
ID=52105120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020167001293A Ceased KR20160034901A (ko) | 2013-06-17 | 2014-06-10 | 서열 조작에 최적화된 crispr-cas 이중 닉카아제 시스템, 방법 및 조성물 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10711285B2 (enExample) |
| EP (2) | EP3011030B1 (enExample) |
| JP (4) | JP6665088B2 (enExample) |
| KR (1) | KR20160034901A (enExample) |
| CN (1) | CN105492611A (enExample) |
| AU (1) | AU2014281027A1 (enExample) |
| CA (1) | CA2915837A1 (enExample) |
| WO (1) | WO2014204725A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019088496A3 (ko) * | 2017-10-31 | 2019-06-27 | 주식회사 에이치유비바이오텍 | 내인성 폴리펩타이드 생산을 위한 재조합 세포 및 방법 |
| WO2022169235A1 (ko) * | 2021-02-04 | 2022-08-11 | 연세대학교 산학협력단 | 교정 효율이 개선된 프라임에디팅용 조성물 |
Families Citing this family (363)
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| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP2766498B1 (en) | 2011-10-14 | 2019-06-19 | President and Fellows of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| ES2728782T3 (es) | 2012-05-25 | 2019-10-28 | Univ California | Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN |
| EP4397759A3 (en) * | 2012-10-23 | 2024-10-09 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| DK3363902T3 (da) | 2012-12-06 | 2020-01-02 | Sigma Aldrich Co Llc | Crispr-baseret genommodifikation og -regulering |
| KR102955556B1 (ko) | 2012-12-12 | 2026-04-22 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물 |
| SG10201707569YA (en) | 2012-12-12 | 2017-10-30 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications |
| WO2014093709A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| IL308158A (en) * | 2012-12-17 | 2023-12-01 | Harvard College | Rna-guided human genome engineering |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
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| KR20250012194A (ko) | 2013-06-17 | 2025-01-23 | 더 브로드 인스티튜트, 인코퍼레이티드 | 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용 |
| US11306328B2 (en) * | 2013-07-26 | 2022-04-19 | President And Fellows Of Harvard College | Genome engineering |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019088496A3 (ko) * | 2017-10-31 | 2019-06-27 | 주식회사 에이치유비바이오텍 | 내인성 폴리펩타이드 생산을 위한 재조합 세포 및 방법 |
| WO2022169235A1 (ko) * | 2021-02-04 | 2022-08-11 | 연세대학교 산학협력단 | 교정 효율이 개선된 프라임에디팅용 조성물 |
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| US12553065B2 (en) | 2026-02-17 |
| JP2016521994A (ja) | 2016-07-28 |
| US20200377912A1 (en) | 2020-12-03 |
| JP6665088B2 (ja) | 2020-03-13 |
| JP7383062B2 (ja) | 2023-11-17 |
| US20160153006A1 (en) | 2016-06-02 |
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