WO2019088496A3 - 내인성 폴리펩타이드 생산을 위한 재조합 세포 및 방법 - Google Patents

내인성 폴리펩타이드 생산을 위한 재조합 세포 및 방법 Download PDF

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WO2019088496A3
WO2019088496A3 PCT/KR2018/012051 KR2018012051W WO2019088496A3 WO 2019088496 A3 WO2019088496 A3 WO 2019088496A3 KR 2018012051 W KR2018012051 W KR 2018012051W WO 2019088496 A3 WO2019088496 A3 WO 2019088496A3
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animal cell
recombinant cell
endogenous polypeptide
producing
producing endogenous
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PCT/KR2018/012051
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French (fr)
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WO2019088496A2 (ko
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배상수
우재성
유지현
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주식회사 에이치유비바이오텍
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Publication of WO2019088496A2 publication Critical patent/WO2019088496A2/ko
Publication of WO2019088496A3 publication Critical patent/WO2019088496A3/ko

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Biochemistry (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

표적특이적 엔도뉴클레아제 시스템 또는 이의 암호화 유전자 및 도너 DNA 구조체를 포함하는 동물 세포에서의 목적 폴리펩타이드 발현 및 /또는 생산용 조성물, 상기 조성물이 도입된 재조합 동물 세포 및 이의 제조 방법, 및 상기 조성물을 동물 세포에 도입하는 단계 및 /또는 상기 동물 세포를 배양하는 단계를 포함하는 동물세포에서의 목적 폴리펩타이드의 발현 및 /또는 생산 방법이 제공된다.
PCT/KR2018/012051 2017-10-31 2018-10-12 내인성 폴리펩타이드 생산을 위한 재조합 세포 및 방법 WO2019088496A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20170143973 2017-10-31
KR10-2017-0143973 2017-10-31

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WO2019088496A2 WO2019088496A2 (ko) 2019-05-09
WO2019088496A3 true WO2019088496A3 (ko) 2019-06-27

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PCT/KR2018/012051 WO2019088496A2 (ko) 2017-10-31 2018-10-12 내인성 폴리펩타이드 생산을 위한 재조합 세포 및 방법

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KR (1) KR102061251B1 (ko)
WO (1) WO2019088496A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102152189B1 (ko) * 2020-02-11 2020-09-04 재단법인 아산사회복지재단 T 세포 내 표적 위치로의 t 세포 수용체 삽입을 위한 재조합벡터 및 이를 이용한 t 세포 내 표적 위치로의 t 세포 수용체 삽입용 조성물

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160034901A (ko) * 2013-06-17 2016-03-30 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작에 최적화된 ｃｒｉｓｐｒ-ｃａｓ 이중 닉카아제 시스템, 방법 및 조성물
WO2016075662A2 (en) * 2014-11-15 2016-05-19 Zumutor Biologics, Inc. Dna-binding domain, non-fucosylated and partially fucosylated proteins, and methods thereof
WO2017024319A1 (en) * 2015-08-06 2017-02-09 Dana-Farber Cancer Institute, Inc. Tunable endogenous protein degradation
KR20170030729A (ko) * 2015-09-09 2017-03-20 연세대학교 산학협력단 Fmr1 유전자를 타겟으로 하는 엔도뉴클레아제를 이용한 cgg 반복의 교정

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140273235A1 (en) 2013-03-15 2014-09-18 Regents Of The University Of Minnesota ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS
JP7019233B2 (ja) 2013-07-11 2022-02-15 モデルナティエックス インコーポレイテッド ＣＲＩＳＰＲ関連タンパク質をコードする合成ポリヌクレオチドおよび合成ｓｇＲＮＡを含む組成物ならびに使用方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160034901A (ko) * 2013-06-17 2016-03-30 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작에 최적화된 ｃｒｉｓｐｒ-ｃａｓ 이중 닉카아제 시스템, 방법 및 조성물
WO2016075662A2 (en) * 2014-11-15 2016-05-19 Zumutor Biologics, Inc. Dna-binding domain, non-fucosylated and partially fucosylated proteins, and methods thereof
WO2017024319A1 (en) * 2015-08-06 2017-02-09 Dana-Farber Cancer Institute, Inc. Tunable endogenous protein degradation
KR20170030729A (ko) * 2015-09-09 2017-03-20 연세대학교 산학협력단 Fmr1 유전자를 타겟으로 하는 엔도뉴클레아제를 이용한 cgg 반복의 교정

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MATSUNAGA, T.: "Single-step generation of gene knockout-rescue system in pluripotent stem cells by promoter insertion with CRISPR/Cas9", BIOCHEM. BIOPHYS. RES. COMMUN., 2014, pages 158 - 163, XP028614859, DOI: doi:10.1016/j.bbrc.2014.01.037 *

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WO2019088496A2 (ko) 2019-05-09
KR20190049456A (ko) 2019-05-09

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