KR102159818B1 - 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법 - Google Patents

시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법 Download PDF

Info

Publication number
KR102159818B1
KR102159818B1 KR1020197034264A KR20197034264A KR102159818B1 KR 102159818 B1 KR102159818 B1 KR 102159818B1 KR 1020197034264 A KR1020197034264 A KR 1020197034264A KR 20197034264 A KR20197034264 A KR 20197034264A KR 102159818 B1 KR102159818 B1 KR 102159818B1
Authority
KR
South Korea
Prior art keywords
dna
nucleic acid
modified
target
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020197034264A
Other languages
English (en)
Korean (ko)
Other versions
KR20190132570A (ko
Inventor
대니얼 쉐퍼
스티븐 에이. 쥬디스
Original Assignee
엔바이로로직스 인크.
대니얼 쉐퍼
스티븐 에이. 쥬디스
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49328090&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR102159818(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 엔바이로로직스 인크., 대니얼 쉐퍼, 스티븐 에이. 쥬디스 filed Critical 엔바이로로직스 인크.
Priority to KR1020207027096A priority Critical patent/KR102362649B1/ko
Publication of KR20190132570A publication Critical patent/KR20190132570A/ko
Application granted granted Critical
Publication of KR102159818B1 publication Critical patent/KR102159818B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020197034264A 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법 Active KR102159818B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020207027096A KR102362649B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261621975P 2012-04-09 2012-04-09
US61/621,975 2012-04-09
PCT/US2013/035750 WO2013155056A1 (en) 2012-04-09 2013-04-09 Compositions and methods for quantifying a nucleic acid sequence in a sample

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020157016789A Division KR102048946B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020207027096A Division KR102362649B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법

Publications (2)

Publication Number Publication Date
KR20190132570A KR20190132570A (ko) 2019-11-27
KR102159818B1 true KR102159818B1 (ko) 2020-09-25

Family

ID=49328090

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020197034264A Active KR102159818B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
KR1020207027096A Active KR102362649B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
KR1020147031343A Active KR101570951B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
KR1020157016789A Active KR102048946B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법

Family Applications After (3)

Application Number Title Priority Date Filing Date
KR1020207027096A Active KR102362649B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
KR1020147031343A Active KR101570951B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
KR1020157016789A Active KR102048946B1 (ko) 2012-04-09 2013-04-09 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법

Country Status (18)

Country Link
US (8) US9096897B2 (enExample)
EP (3) EP4524265A3 (enExample)
JP (4) JP5801513B2 (enExample)
KR (4) KR102159818B1 (enExample)
CN (2) CN104685066B (enExample)
AU (3) AU2013246080C1 (enExample)
BR (1) BR112014025262A8 (enExample)
CA (1) CA2869971C (enExample)
DK (2) DK3587587T3 (enExample)
ES (2) ES2743050T5 (enExample)
FI (1) FI3587587T3 (enExample)
IN (1) IN2014DN08831A (enExample)
MX (2) MX367666B (enExample)
NZ (2) NZ723570A (enExample)
RU (2) RU2601129C2 (enExample)
UA (2) UA125112C2 (enExample)
WO (1) WO2013155056A1 (enExample)
ZA (3) ZA201408116B (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2810856C (en) 2010-08-13 2022-05-17 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
IN2014DN08831A (enExample) 2012-04-09 2015-05-22 Envirologix Inc
UA123387C2 (uk) 2014-04-22 2021-03-31 Інвайролоджикс, Інк. Виділений праймерний олігонуклеотид та спосіб його застосування
CN106574299A (zh) * 2014-04-30 2017-04-19 龙公司 用来检测黄龙病的组合物和方法
ES2759337T3 (es) * 2014-10-14 2020-05-08 Abbott Lab ADN de conversión de secuencia y ADN amplificador de señal que tiene secuencias espaciadoras de poli-ADN y métodos de detección que los utilizan
EP4141128A1 (en) * 2014-10-20 2023-03-01 Envirologix Inc. Compositions and methods for detecting an rna virus
WO2016122698A1 (en) * 2015-01-30 2016-08-04 Envirologix, Inc. Compositions and methods for rapid detection of salmonella
WO2016123029A1 (en) 2015-01-30 2016-08-04 Envirologix Inc. Substrate molecule
KR102782598B1 (ko) * 2015-06-25 2025-03-14 로스웰 엠이 아이엔씨. 바이오분자 센서들 및 방법들
EP3325495A1 (en) * 2015-07-20 2018-05-30 Envirologix Inc. Compositions and methods for rapid detection of salmonella serovar d1
EP3368654B1 (en) * 2015-10-30 2025-11-19 Abbott Diagnostics Scarborough, Inc. Determination of polymorphisms using isothermal nucleic acid amplification
US10329601B2 (en) 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
US10712334B2 (en) 2016-01-28 2020-07-14 Roswell Biotechnologies, Inc. Massively parallel DNA sequencing apparatus
WO2017132586A1 (en) 2016-01-28 2017-08-03 Roswell Biotechnologies, Inc. Methods and apparatus for measuring analytes using large scale molecular electronics sensor arrays
EP3882616A1 (en) 2016-02-09 2021-09-22 Roswell Biotechnologies, Inc Electronic label-free dna and genome sequencing
US10597767B2 (en) 2016-02-22 2020-03-24 Roswell Biotechnologies, Inc. Nanoparticle fabrication
GB201611469D0 (en) * 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
US9829456B1 (en) 2016-07-26 2017-11-28 Roswell Biotechnologies, Inc. Method of making a multi-electrode structure usable in molecular sensing devices
CN110431148A (zh) 2017-01-10 2019-11-08 罗斯威尔生命技术公司 用于dna数据存储的方法和系统
CN110520517A (zh) 2017-01-19 2019-11-29 罗斯威尔生命技术公司 包括二维层材料的固态测序装置
EP3615685B1 (en) 2017-04-25 2025-02-19 Roswell Biotechnologies, Inc Enzymatic circuits for molecular sensors
US10508296B2 (en) 2017-04-25 2019-12-17 Roswell Biotechnologies, Inc. Enzymatic circuits for molecular sensors
WO2018208505A1 (en) 2017-05-09 2018-11-15 Roswell Biotechnologies, Inc. Binding probe circuits for molecular sensors
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
TWI754652B (zh) * 2017-07-10 2022-02-11 英商盧米瑞德克斯英國有限公司 核酸擴增方法中或相關之改良
EP3676389A4 (en) 2017-08-30 2021-06-02 Roswell Biotechnologies, Inc PROCESSIVE ENZYMATIC MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA
WO2019046610A1 (en) * 2017-08-31 2019-03-07 Ionian Technologies Inc. REACTION OF SYNCHRONIZATION AMPLIFICATION AND (NEAR) EXTENSION OF SYNCYTIAL RESPIRATORY VIRUS SPECIES
KR102717872B1 (ko) 2017-10-10 2024-10-14 로스웰 엠이 아이엔씨. 무증폭 dna 데이터 저장을 위한 방법, 장치 및 시스템
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CN108588252A (zh) * 2018-03-29 2018-09-28 杭州泰熙生物技术有限公司 一种寄生虫检测试剂盒及其检测方法
US12319712B2 (en) 2018-05-09 2025-06-03 Vibrant Holdings, Llc Methods of synthesizing a polynucleotide array using photoactivated agents
BR112020026246A2 (pt) * 2018-06-26 2021-04-20 The Broad Institute Inc. composições, sistemas e métodos de amplificação com base em nickase dupla crispr
GB201812149D0 (en) * 2018-07-25 2018-09-05 Sense Biodetection Ltd Nucleic acid detection method
KR102154628B1 (ko) 2019-01-03 2020-09-10 베트올 (주) 반정량 수평류 면역진단 기술을 이용한 랄스토니아 솔라나세아룸과 푸사리움 옥시스포룸의 동시 진단 방법
KR102199394B1 (ko) * 2019-07-01 2021-01-06 한국화학연구원 이중분자비콘과 산화그래핀을 포함하는 핵산 검출용 조성물 및 이를 이용한 핵산 검출의 비색신호 증폭 방법
KR102531590B1 (ko) * 2019-07-16 2023-05-11 고려대학교 산학협력단 절단효소 인식부위의 미러링 결합에 의한 표적핵산의 검출 방법 및 핵산 검출용 조성물
CN110408678A (zh) * 2019-07-26 2019-11-05 宁儿医院股份有限公司 单管型多重微生物检测体系及其即时检测方法
EP4146815A1 (en) * 2020-05-07 2023-03-15 Bioskryb Genomics, Inc. Detection of low abundance nucleic acids
KR20230141826A (ko) * 2021-02-02 2023-10-10 로디엑스, 인코포레이티드 합성 폴리뉴클레오티드 및 대립유전자를 선택적으로 증폭하기 위한 방법
CN118541495A (zh) * 2021-12-08 2024-08-23 麻省理工学院 多核苷酸的低温等温扩增
GB2635849A (en) * 2022-05-14 2025-05-28 Jiangsu Code Biomedical Tech Co Ltd Novel compositions and methods for cell-free DNA detection
TW202421795A (zh) * 2022-07-01 2024-06-01 美商夏洛克生物科學公司 環境溫度核酸擴增及偵測
WO2024030985A1 (en) 2022-08-04 2024-02-08 Abbott Laboratories Assays for detecting monkeypox virus
WO2025049450A1 (en) 2023-08-28 2025-03-06 Abbott Molecular, Inc. Assays for detecting bk virus (bkv)
WO2025049451A1 (en) 2023-08-28 2025-03-06 Abbott Molecular, Inc. Assays for detecting cytomegalovirus (cmv) or epstein-bar virus (ebv)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003008622A2 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Exponential nucleic acid amplification using nicking endonucleases
US20030040620A1 (en) 2000-05-20 2003-02-27 Langmore John P. Method of producing a DNA library using positional amplification
WO2006074162A2 (en) 2005-01-04 2006-07-13 Hitachi Chemical Company, Ltd. Primer generation rolling circle amplification
WO2008002920A2 (en) 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
US20090017453A1 (en) 2007-07-14 2009-01-15 Maples Brian K Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2012021493A2 (en) 2010-08-13 2012-02-16 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2012022755A1 (en) 2010-08-17 2012-02-23 Qiagen Gmbh Helicase dependent isothermal amplification using nicking enzymes

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
WO1994003635A1 (en) 1992-08-04 1994-02-17 Institute Of Molecular Biology & Biotechnology Rapid amplification and detection of nucleic acids
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5629179A (en) 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
JP2000505312A (ja) * 1996-03-18 2000-05-09 モレキュラー バイオロジー リソーシーズ，インコーポレイテッド 標的核酸配列増幅
US6130038A (en) * 1996-07-16 2000-10-10 Gen-Probe Incorporated Method for amplifying target nucleic acids using modified primers
GB9716231D0 (en) 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
EP1025120B1 (en) 1997-10-27 2010-08-18 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US5952202A (en) 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
AU1908100A (en) 1998-11-06 2000-05-29 Molecular Biology Resources, Inc. Isothermal nucleic acid amplification methods
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6191267B1 (en) 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
DE60115811T2 (de) * 2000-10-25 2006-08-03 Roche Diagnostics Gmbh Amplifizierung unter Verwendung von modifizierten Primern
JP2004532615A (ja) * 2000-12-27 2004-10-28 インヴィトロジェン コーポレーション 核酸の検出および識別のためのプライマーおよび方法
US7112423B2 (en) 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
US7056671B2 (en) 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20030211483A1 (en) 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
WO2004067726A2 (en) 2003-01-29 2004-08-12 Keck Graduate Institute Isothermal reactions for the amplification of oligonucleotides
JP4380305B2 (ja) 2003-11-21 2009-12-09 味の素株式会社 発酵法によるｌ−アミノ酸の製造法
US20090017452A1 (en) 2004-03-05 2009-01-15 University Of Chicago Methods and compositions relating to the pharmacogenetics of different gene variants
US20060115838A1 (en) 2004-10-19 2006-06-01 Trevigen Inc. Real-time detection of amplicons using nucleic acid repair enzymes
JP4555136B2 (ja) * 2005-03-31 2010-09-29 本田技研工業株式会社 燃料電池の電気システム、燃料電池車両及び電力供給方法
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
US20070031857A1 (en) 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7947286B2 (en) 2005-10-07 2011-05-24 Panthera Biopharma Llc Drosophila cell lines producing recombinant secretable filovirus surface glycoproteins lacking the membrane spanning domain
US20080096257A1 (en) 2006-08-15 2008-04-24 Zuxu Yao Methods for Rapid, Single-Step Strand Displacement Amplification of Nucleic Acids
DE102006039396A1 (de) 2006-08-22 2008-03-13 Robert Bosch Gmbh Laserlichtquelle
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
EP2066686A4 (en) 2006-10-06 2010-07-14 Dna Genotek Inc STABILIZING COMPOSITIONS AND METHODS FOR EXTRACTING A RIBONUCLEIC ACID
JP2008136451A (ja) 2006-12-05 2008-06-19 Hitachi Ltd 核酸増幅法
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
CL2008000083A1 (es) † 2007-01-12 2008-07-18 Monsanto Technology Llc Soc Or Polipeptido cristalizado de dicamba monooxigenasa (dmo); molecula que comprende una superficie de union para dicamba; celula vegetal que comprende dmo.
US20080254458A1 (en) 2007-04-10 2008-10-16 Asiagen Corporation Method of pre-treating in pleural effusion for detection of Mycobacterium tuberculosis
US20080274458A1 (en) 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
WO2009020609A2 (en) 2007-08-06 2009-02-12 Nanogen, Inc. Isolation of nucleic acids molecules using modified solid supports
US20090197254A1 (en) 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
JPWO2010026933A1 (ja) 2008-09-03 2012-02-02 タカラバイオ株式会社 Ｒｎａ検出用組成物
CA2749939A1 (en) 2009-01-21 2010-07-29 Human Genetic Signatures Pty Ltd Improved isothermal strand displacement amplification
EP2408936A4 (en) 2009-03-18 2013-01-30 Sequenom Inc USE OF HEAT-STAINED ENDONUCLEASES FOR THE PREPARATION OF REPORTER MOLECULES
WO2010126913A1 (en) 2009-04-27 2010-11-04 Gen-Probe Incorporated Methods and kits for use in the selective amplification of target sequences
CA2849023C (en) 2011-09-15 2022-07-19 David A. Shafer Probe:antiprobe compositions for high specificity dna or rna detection
US20130217071A1 (en) 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
IN2014DN08831A (enExample) 2012-04-09 2015-05-22 Envirologix Inc
CN104583413B (zh) 2012-06-29 2018-10-19 通用电气公司 用于自rna模板开始的等温dna扩增试剂盒
JP2014082936A (ja) 2012-10-19 2014-05-12 Kyoto Univ 変異型逆転写酵素
UA123387C2 (uk) 2014-04-22 2021-03-31 Інвайролоджикс, Інк. Виділений праймерний олігонуклеотид та спосіб його застосування
CN106574299A (zh) 2014-04-30 2017-04-19 龙公司 用来检测黄龙病的组合物和方法
EP4141128A1 (en) 2014-10-20 2023-03-01 Envirologix Inc. Compositions and methods for detecting an rna virus
BR112017008718A2 (pt) 2014-10-28 2017-12-19 Envirologix Inc ?recipientes de preparação de amostra, circuitos de microfluídos, e sistemas e métodos para preparação, extração e análise da amostra?
WO2016122698A1 (en) 2015-01-30 2016-08-04 Envirologix, Inc. Compositions and methods for rapid detection of salmonella

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030040620A1 (en) 2000-05-20 2003-02-27 Langmore John P. Method of producing a DNA library using positional amplification
WO2003008622A2 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Exponential nucleic acid amplification using nicking endonucleases
WO2006074162A2 (en) 2005-01-04 2006-07-13 Hitachi Chemical Company, Ltd. Primer generation rolling circle amplification
WO2008002920A2 (en) 2006-06-26 2008-01-03 Epoch Biosciences, Inc. Methods for generating target nucleic acid sequences
US20090017453A1 (en) 2007-07-14 2009-01-15 Maples Brian K Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2012021493A2 (en) 2010-08-13 2012-02-16 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
WO2012022755A1 (en) 2010-08-17 2012-02-23 Qiagen Gmbh Helicase dependent isothermal amplification using nicking enzymes

Also Published As

Publication number Publication date
EP2836609B1 (en) 2019-05-22
US20200239947A1 (en) 2020-07-30
IN2014DN08831A (enExample) 2015-05-22
JP2016073273A (ja) 2016-05-12
UA116205C2 (uk) 2018-02-26
JP6169660B2 (ja) 2017-07-26
ES2743050T5 (es) 2022-10-20
KR20150031229A (ko) 2015-03-23
US20160122811A1 (en) 2016-05-05
FI3587587T3 (fi) 2025-01-30
US11208687B2 (en) 2021-12-28
ZA201508197B (en) 2017-05-31
CA2869971C (en) 2022-10-25
RU2601129C2 (ru) 2016-10-27
RU2016138585A (ru) 2018-12-13
JP5801513B2 (ja) 2015-10-28
CA2869971A1 (en) 2013-10-17
US20150315635A1 (en) 2015-11-05
JP2018007658A (ja) 2018-01-18
US10077467B2 (en) 2018-09-18
WO2013155056A1 (en) 2013-10-17
JP6321738B2 (ja) 2018-05-09
US20170166960A1 (en) 2017-06-15
NZ723570A (en) 2018-06-29
AU2013246080C1 (en) 2018-03-29
KR101570951B1 (ko) 2015-11-23
KR20190132570A (ko) 2019-11-27
US9096897B2 (en) 2015-08-04
AU2013246080B2 (en) 2015-07-23
RU2014144722A (ru) 2016-06-10
NZ701145A (en) 2016-09-30
BR112014025262A8 (pt) 2021-06-29
US20220243262A1 (en) 2022-08-04
KR102362649B1 (ko) 2022-02-14
RU2016138585A3 (enExample) 2020-03-19
ES3011408T3 (en) 2025-04-07
US20180363046A1 (en) 2018-12-20
ZA201408116B (en) 2016-02-24
CN104685066A (zh) 2015-06-03
EP2836609A4 (en) 2015-11-11
JP6480511B2 (ja) 2019-03-13
CN114134207A (zh) 2022-03-04
US10584376B2 (en) 2020-03-10
WO2013155056A9 (en) 2014-01-09
MX2014012214A (es) 2015-05-08
AU2013246080A1 (en) 2014-11-13
US20150104788A1 (en) 2015-04-16
HK1210228A1 (en) 2016-04-15
KR20200111287A (ko) 2020-09-28
EP2836609B2 (en) 2022-06-15
US20180094310A1 (en) 2018-04-05
RU2732589C2 (ru) 2020-09-21
ZA201607642B (en) 2018-05-30
EP4524265A2 (en) 2025-03-19
JP2015512654A (ja) 2015-04-30
DK3587587T3 (da) 2025-02-03
MX338296B (es) 2016-04-11
US11866773B2 (en) 2024-01-09
AU2018201671A1 (en) 2018-04-05
EP3587587B1 (en) 2024-11-06
JP2017029154A (ja) 2017-02-09
DK2836609T4 (da) 2022-09-05
EP4524265A3 (en) 2025-06-25
KR102048946B1 (ko) 2019-11-27
EP3587587A1 (en) 2020-01-01
MX367666B (es) 2019-08-30
US9631231B2 (en) 2017-04-25
KR20150080023A (ko) 2015-07-08
US9322053B2 (en) 2016-04-26
AU2015246059A1 (en) 2015-11-05
ES2743050T3 (es) 2020-02-18
AU2018201671B2 (en) 2019-11-14
DK2836609T3 (da) 2019-08-26
UA125112C2 (uk) 2022-01-12
BR112014025262A2 (pt) 2017-06-20
CN104685066B (zh) 2021-09-21
EP2836609A1 (en) 2015-02-18
AU2015246059B2 (en) 2017-12-07

Similar Documents

Publication Publication Date Title
KR102159818B1 (ko) 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
HK1210228B (zh) 用於量化样品中核酸序列的组合物和方法

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20191120

Application number text: 1020157016789

Filing date: 20150623

PG1501 Laying open of application
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20191219

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20200113

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20200618

PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20200918

Application number text: 1020157016789

Filing date: 20150623

PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20200918

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20200921

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20240823

Start annual number: 5

End annual number: 5