CA2869971C - Methods for quantifying a nucleic acid in a sample using a nicking amplification and detection reaction - Google Patents

Methods for quantifying a nucleic acid in a sample using a nicking amplification and detection reaction Download PDF

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Publication number
CA2869971C
CA2869971C CA2869971A CA2869971A CA2869971C CA 2869971 C CA2869971 C CA 2869971C CA 2869971 A CA2869971 A CA 2869971A CA 2869971 A CA2869971 A CA 2869971A CA 2869971 C CA2869971 C CA 2869971C
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nucleic acid
target nucleic
acid molecule
sequence
modified nucleotides
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CA2869971A1 (en
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Daniel Shaffer
Stephen A. Judice
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Envirologix Inc
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Envirologix Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2869971A 2012-04-09 2013-04-09 Methods for quantifying a nucleic acid in a sample using a nicking amplification and detection reaction Active CA2869971C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261621975P 2012-04-09 2012-04-09
US61/621,975 2012-04-09
PCT/US2013/035750 WO2013155056A1 (en) 2012-04-09 2013-04-09 Compositions and methods for quantifying a nucleic acid sequence in a sample

Publications (2)

Publication Number Publication Date
CA2869971A1 CA2869971A1 (en) 2013-10-17
CA2869971C true CA2869971C (en) 2022-10-25

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CA2869971A Active CA2869971C (en) 2012-04-09 2013-04-09 Methods for quantifying a nucleic acid in a sample using a nicking amplification and detection reaction

Country Status (18)

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US (8) US9096897B2 (enExample)
EP (3) EP2836609B2 (enExample)
JP (4) JP5801513B2 (enExample)
KR (4) KR101570951B1 (enExample)
CN (2) CN114134207B (enExample)
AU (3) AU2013246080C1 (enExample)
BR (1) BR112014025262A8 (enExample)
CA (1) CA2869971C (enExample)
DK (2) DK3587587T3 (enExample)
ES (2) ES3011408T3 (enExample)
FI (1) FI3587587T3 (enExample)
IN (1) IN2014DN08831A (enExample)
MX (2) MX338296B (enExample)
NZ (2) NZ723570A (enExample)
RU (2) RU2732589C2 (enExample)
UA (2) UA125112C2 (enExample)
WO (1) WO2013155056A1 (enExample)
ZA (3) ZA201408116B (enExample)

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