KR101961958B1 - 세포로부터의 지질의 추출 및 이로부터의 생성물 - Google Patents

세포로부터의 지질의 추출 및 이로부터의 생성물 Download PDF

Info

Publication number
KR101961958B1
KR101961958B1 KR1020127034055A KR20127034055A KR101961958B1 KR 101961958 B1 KR101961958 B1 KR 101961958B1 KR 1020127034055 A KR1020127034055 A KR 1020127034055A KR 20127034055 A KR20127034055 A KR 20127034055A KR 101961958 B1 KR101961958 B1 KR 101961958B1
Authority
KR
South Korea
Prior art keywords
less
cell composition
lipid
hours
dissolved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020127034055A
Other languages
English (en)
Korean (ko)
Other versions
KR20130108103A (ko
Inventor
스티븐 로버트 체린코
로버트 코디 커티스
커크 이 압트
폴 워렌 버렌스
존 밀톤 한센
조셉 더블유 3세 파이퍼
트레이시 린 스탈
로스 저클
나스린 타바예 버크
크리쉬나 라만
네일 프란시스 라이닌저
Original Assignee
디에스엠 아이피 어셋츠 비.브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=45022637&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR101961958(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 디에스엠 아이피 어셋츠 비.브이. filed Critical 디에스엠 아이피 어셋츠 비.브이.
Publication of KR20130108103A publication Critical patent/KR20130108103A/ko
Application granted granted Critical
Publication of KR101961958B1 publication Critical patent/KR101961958B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/16—Refining fats or fatty oils by mechanical means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Mechanical Engineering (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Edible Oils And Fats (AREA)
KR1020127034055A 2010-06-01 2011-06-01 세포로부터의 지질의 추출 및 이로부터의 생성물 Active KR101961958B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US35036310P 2010-06-01 2010-06-01
US61/350,363 2010-06-01
US37892310P 2010-08-31 2010-08-31
US61/378,923 2010-08-31
US201161452721P 2011-03-15 2011-03-15
US61/452,721 2011-03-15
PCT/US2011/038768 WO2011153246A2 (en) 2010-06-01 2011-06-01 Extraction of lipid from cells and products therefrom

Publications (2)

Publication Number Publication Date
KR20130108103A KR20130108103A (ko) 2013-10-02
KR101961958B1 true KR101961958B1 (ko) 2019-03-25

Family

ID=45022637

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020127034055A Active KR101961958B1 (ko) 2010-06-01 2011-06-01 세포로부터의 지질의 추출 및 이로부터의 생성물

Country Status (10)

Country Link
US (3) US10392578B2 (enExample)
EP (2) EP2576801B1 (enExample)
JP (2) JP5911479B2 (enExample)
KR (1) KR101961958B1 (enExample)
CN (1) CN103124791B (enExample)
AR (1) AR081558A1 (enExample)
AU (4) AU2011261455B2 (enExample)
CA (1) CA2801011C (enExample)
ES (1) ES2758782T3 (enExample)
WO (1) WO2011153246A2 (enExample)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2295595B1 (en) 2000-01-19 2019-05-01 DSM IP Assets B.V. Solventless extraction process
NZ601757A (en) 2010-01-19 2015-01-30 Dsm Ip Assets Bv Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
EP2576801B1 (en) 2010-06-01 2019-10-02 DSM IP Assets B.V. Extraction of lipid from cells and products therefrom
US9028696B2 (en) * 2010-07-26 2015-05-12 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds from biomass
FR2975705B1 (fr) * 2011-05-27 2014-12-26 Roquette Freres Procede d'extraction du squalene a partir de microalgues
EP2952183A1 (en) 2011-07-21 2015-12-09 DSM IP Assets B.V. Fatty acid compositions
JP2014530614A (ja) * 2011-10-20 2014-11-20 フリンダーズユニバーシティ オブ サウス オーストラリアFlinders University of South Australia 微細藻類の抽出
KR20200003227A (ko) 2011-11-01 2020-01-08 디에스엠 아이피 어셋츠 비.브이. 산화적으로 안정한 다중불포화된 지방산 함유 오일
JP6220388B2 (ja) * 2012-05-25 2017-10-25 ヘルス・ダイアグノスティック・ラボラトリー，インコーポレーテッド ステロール／スタノール又はそれらの誘導体の迅速でハイスループットの分析
CN104508113A (zh) 2012-06-29 2015-04-08 Bp生物燃料英国有限公司 从微生物中分离可再生物质的方法
SG11201605052XA (en) * 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
ES2984970T3 (es) 2013-12-20 2024-10-31 Mara Renewables Corp Métodos para recuperar aceite de microorganismos
TWI646189B (zh) * 2013-12-20 2019-01-01 荷蘭商Ｄｓｍ智慧財產有限公司 用於從微生物細胞獲得微生物油之方法（五）
EP3083909A1 (en) * 2013-12-20 2016-10-26 DSM IP Assets B.V. Process for extracting lipids for use in production of biofuels
CN105829539B (zh) * 2013-12-20 2023-11-10 帝斯曼营养品股份公司 从微生物中回收油的方法
AU2014369046B2 (en) 2013-12-20 2019-02-28 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
CA2934490C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
MX370606B (es) * 2013-12-20 2019-12-18 Dsm Ip Assets Bv Procedimientos para obtener aceite microbiano a partir de células microbianas.
EP3107985A4 (en) * 2014-02-17 2017-08-09 University of Maryland, Baltimore Method of extracting lipids from microbes
JP6611136B2 (ja) * 2014-02-28 2019-11-27 デルフト アドヴァンスト バイオフューエルズ ベー．フェー． 脂質または炭化水素の回収のための方法
CN105567715B (zh) * 2014-10-17 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌α-微管蛋白相关序列及其应用
JP6866301B2 (ja) * 2015-01-12 2021-04-28 ステパン カンパニー ラムノリピド組成物の製造
CN106554273B (zh) * 2015-09-30 2020-02-14 中国石油化工股份有限公司 一种发酵液中长链二羧酸的提纯方法
IL245059A0 (en) * 2016-04-12 2016-06-30 Technion Res & Dev Foundation Electrospun scaffolds from extracellular matrix
ES2930078T3 (es) 2016-05-12 2022-12-07 Dsm Ip Assets Bv Método para incrementar la producción de ácidos grasos poliinsaturados omega-3 en microalgas
JP6738214B2 (ja) * 2016-06-17 2020-08-12 アースリサイクル株式会社 バイオ燃料の製造方法
CN107523417A (zh) * 2016-06-21 2017-12-29 嘉必优生物技术（武汉）股份有限公司 提取微生物油脂的方法
KR102405390B1 (ko) * 2016-07-13 2022-06-03 에보닉 오퍼레이션스 게엠베하 용해된 지질을 함유하는 바이오매스로부터 지질을 분리하는 방법
WO2018013670A1 (en) * 2016-07-13 2018-01-18 Dsm Ip Assets B.V. Method for extracting a microbial oil comprising polyunsaturated fatty acids from a fermentation broth containing oleaginous microorganisms
JP6998934B2 (ja) * 2016-07-13 2022-01-18 エボニック オペレーションズ ゲーエムベーハー 脂質含有細胞から脂質を単離する方法
CA3030471C (en) * 2016-07-13 2023-06-20 Evonik Degussa Gmbh Method of separating lipids from a lysed lipids containing biomass
CA3030467C (en) * 2016-07-13 2023-07-11 Evonik Degussa Gmbh Method for isolating lipids from lipid-containing cells
WO2018050255A1 (en) 2016-09-19 2018-03-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Puck and method for manufacturing a puck
WO2018112165A1 (en) * 2016-12-14 2018-06-21 University Of Maryland, Baltimore Methods for lipid extraction and identification of microbes using same via mass spectrometry
DK3562925T3 (da) 2016-12-27 2021-05-25 Evonik Operations Gmbh Fremgangsmåde til isolering af lipider fra en lipidholdig biomasse
EP3562576B1 (en) * 2016-12-29 2023-09-06 Intus Biosciences, LLC Combined lysis protocol for comprehensive cell lysis
US11149246B2 (en) 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
WO2019030073A1 (en) 2017-08-07 2019-02-14 Total Raffinage Chimie PROCESS FOR RECOVERING ISOPRENOIDS PRODUCED BY MICROORGANISMS
CA3072846A1 (en) 2017-08-17 2019-02-21 Evonik Operations Gmbh Enhanced production of lipids by limitation of at least two limiting nutrient sources
WO2019034354A1 (en) 2017-08-17 2019-02-21 Evonik Degussa Gmbh ENHANCED PRODUCTION OF LIPIDS BY LIMITING AT LEAST TWO SOURCES OF NUTRIENT LIMITING
EP3470502A1 (en) 2017-10-13 2019-04-17 Evonik Degussa GmbH Method of separating lipids from a lysed lipids containing biomass
WO2019121752A1 (en) * 2017-12-20 2019-06-27 Evonik Degussa Gmbh Method of isolating lipids from a lipids containing biomass
EP3527664A1 (en) * 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
CA3084705A1 (en) * 2017-12-22 2019-06-27 Dsm Ip Assets B.V. Oil comprising at least one polyunsaturated fatty acid having at least 20 carbon atoms (lc-pufa)
WO2019191545A1 (en) * 2018-03-30 2019-10-03 Dsm Ip Assets B.V. Method of reducing emulsion by broth washing
BR112020019931A2 (pt) * 2018-03-30 2021-03-30 Dsm Ip Assets B.V. Método de redução de emulsão através da manutenção de uma baixa concentração de carboidrato
BR112020023222A2 (pt) 2018-05-15 2021-03-23 Evonik Operations Gmbh método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão
CN112166176B (zh) * 2018-05-15 2024-06-25 赢创运营有限公司 借助疏水性二氧化硅从含有脂质的生物质中分离脂质的方法
WO2020016363A1 (en) 2018-07-20 2020-01-23 Total Raffinage Chimie Wet process for recovering oil produced by microorganism
FR3085962B1 (fr) * 2018-09-14 2021-06-18 Fermentalg Procede d'extracton d'une huile riche en pufa
FR3085825B1 (fr) 2018-09-14 2021-07-16 Fermentalg Huile de microorganismes riche en acide docosahexaenoique
BR112021008853A2 (pt) 2018-11-09 2021-08-17 Evonik Operations Gmbh método para produzir uma biomassa que pode ser facilmente rompida e que tem um teor aumentado de ácidos graxos poliinsaturados
CA3118527A1 (en) 2018-11-09 2020-05-14 Evonik Operations Gmbh Method for producing a biomass with an increased content of polyunsaturated fatty acids
EP3938480A4 (en) * 2019-03-14 2022-12-28 DSM IP Assets B.V. Methods of obtaining lipids from a microbial cell composition by enzyme and ph shock
CN113574045A (zh) * 2019-03-14 2021-10-29 帝斯曼知识产权资产管理有限公司 从微生物细胞组合物中获得脂质的方法
CN110106019A (zh) * 2019-05-20 2019-08-09 无限极（中国）有限公司 一种利用裂壶藻生产复合型多不饱和脂肪酸油脂的方法
CN110172373B (zh) * 2019-06-14 2022-08-05 中国农业科学院农产品加工研究所 白藜芦醇在花生油中的应用
CN110846346B (zh) * 2019-11-26 2021-06-22 瞿瀚鹏 富含Sn-2位DHA的微生物油脂及其制备方法和应用
IL297000A (en) 2020-04-03 2022-12-01 Mara Renewables Corp Microbial oils with high levels of omega-3 fatty acids
FR3111912B1 (fr) 2020-06-24 2025-10-03 Fermentalg Procédé de culture de microorganismes pour l’accumulation de lipides
CN112607826B (zh) * 2021-03-02 2021-05-07 烟台垚磊信息技术有限公司 一种含油乳化废水处理装置
FR3130842B1 (fr) 2021-12-22 2025-06-27 CarbonWorks Procede de captation des phytotoxines dans un reacteur biologique
WO2023214072A2 (en) 2022-05-05 2023-11-09 Dsm Ip Assets B.V. Novel process

Family Cites Families (161)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2753362A (en) 1951-05-18 1956-07-03 Standard Brands Inc Process of extracting lipids from plant and animal tissue
GB808128A (en) 1955-12-01 1959-01-28 Nat Res Dev A method of increasing the fatty contents of such micro-organisms as yeasts, bacteria and moulds
US3089821A (en) 1959-10-28 1963-05-14 Merck & Co Inc Process for the preparation of lipids
DE2056896B2 (de) 1970-02-18 1979-12-06 Veb Schwermaschinenbau Kombinat Ernst Thaelmann Magdeburg, Ddr 3011 Magdeburg Verfahren zur gleichzeitigen Gewinnung von Fett und Protein aus pflanzlichen, ölhaltigen Rohstoffen und Zwischenprodukten
US3878232A (en) 1970-09-28 1975-04-15 Staley Mfg Co A E Extraction process to improve the quality and yield of crude vegetable oils
GB1466853A (en) 1973-05-22 1977-03-09 Simon Rosedowns Ltd Extraction
US4504473A (en) 1982-06-30 1985-03-12 Ribi Immunochem Research, Inc. Pyridine soluble extract of a microorganism
DE3248167A1 (de) 1982-12-27 1984-06-28 Wintershall Ag, 3100 Celle Trehaloselipidtetraester
JPS61170397A (ja) 1985-01-22 1986-08-01 Agency Of Ind Science & Technol モルテイエレラ属糸状菌体の多段抽出処理方法
WO1986004354A1 (en) 1985-01-22 1986-07-31 Japan As Represented By Director General Of Agency Method for obtaining lipids from fungus bodies
US4792418A (en) 1985-08-14 1988-12-20 Century Laboratories, Inc. Method of extraction and purification of polyunsaturated fatty acids from natural sources
JPS6244170A (ja) 1985-08-19 1987-02-26 Agency Of Ind Science & Technol モルテイエレラ属糸状菌体の超臨界流体による抽出方法
US4680314A (en) 1985-08-30 1987-07-14 Microbio Resources, Inc. Process for producing a naturally-derived carotene/oil composition by direct extraction from algae
JPS62278987A (ja) 1986-05-26 1987-12-03 Kanegafuchi Chem Ind Co Ltd 酵素反応生成物の回収方法
DK199887D0 (da) 1987-04-15 1987-04-15 Danisco Bioteknologi As Gaerstamme
JPS63304990A (ja) 1987-06-04 1988-12-13 Meiji Seika Kaisha Ltd 菌体内有効成分の抽出方法
FR2621327B1 (fr) 1987-10-06 1990-01-05 Commissariat Energie Atomique Procede de production et d'extraction de polysaccharides a partir d'une culture de porphyridium cruentum et dispositif pour la mise en oeuvre du procede
JPH0198494A (ja) 1987-10-09 1989-04-17 Agency Of Ind Science & Technol バイオリアクター
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340594A (en) 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
US6451567B1 (en) 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
US5173409A (en) 1989-12-08 1992-12-22 Ecogen Inc. Recovery of bt endotoxin protein from lysed cell mixtures
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
FR2656874B1 (fr) 1990-01-11 1992-04-03 Commissariat Energie Atomique Procede de production et d'extraction d'anti-oxydants a partir d'une culture de micro-organismes et photobioreacteur pour la mise en óoeuvre de ce procede.
US5407957A (en) 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
US5133963A (en) 1990-12-21 1992-07-28 Shuntaro Ise Method of producing commercially useful poultry products with increased concentrations of Omega-3 polyunsaturated fatty acids
US5658767A (en) 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
US6270828B1 (en) 1993-11-12 2001-08-07 Cargrill Incorporated Canola variety producing a seed with reduced glucosinolates and linolenic acid yielding an oil with low sulfur, improved sensory characteristics and increased oxidative stability
FR2686619B1 (fr) 1992-01-28 1995-07-13 Commissariat Energie Atomique Procede de production selective de lipides poly-insatures a partir d'une culture de micro-algues du type porphyridium et cuve utilisee dans ce procede.
US5476787A (en) * 1992-04-24 1995-12-19 Director-General Of Agency Of Industrial Science And Technology Method of removing nitrogen impurities from water using hydrocarbon-producing microalga
DE4219360C2 (de) 1992-06-12 1994-07-28 Milupa Ag Verfahren zur Gewinnung von Lipiden mit einem hohen Anteil von langkettig-hochungesättigten Fettsäuren
CA2161512C (en) 1993-04-27 2002-02-26 Zhegong Fan Non-hydrogenated canola oil for food applications
CA2596241C (en) * 1993-06-09 2011-04-19 Martek Biosciences Corporation Methods and pharmaceutical compositions useful for treating neurological disorders
FR2719222B1 (fr) 1994-05-02 1996-06-21 Rocher Yves Biolog Vegetale Vésicules lipidiques, leur procédé de fabrication et leurs applications.
ATE181745T1 (de) 1994-08-16 1999-07-15 Frische Gmbh Verfahren zur gewinnung von nicht wasserlöslichen, nativen produkten aus nativen stoffgemengen mit hilfe der zentrifugalkraft
KR100212644B1 (ko) 1994-10-29 1999-08-02 토니헬샴 유압식 건설기계의 엔진회전수 제어장치 및 제어방법
US5583019A (en) 1995-01-24 1996-12-10 Omegatech Inc. Method for production of arachidonic acid
WO1996033263A1 (en) 1995-04-17 1996-10-24 JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
JP2764572B2 (ja) 1995-04-17 1998-06-11 工業技術院長 ドコサヘキサエン酸生産能を有する新規微生物及びそれを用いたドコサヘキサエン酸の製造方法
DK0741183T3 (da) 1995-05-04 1999-10-11 Nestle Sa Fremgangsmåde til fraktionering af fede syrer
JPH099981A (ja) 1995-06-28 1997-01-14 Sekiyu Sangyo Kasseika Center 油水二相系微生物反応における油水分離方法
GB9514649D0 (en) 1995-07-18 1995-09-13 Zeneca Ltd Extraction of triglycerides from microorganisms
JP3985035B2 (ja) 1995-09-14 2007-10-03 独立行政法人産業技術総合研究所 （ｎ−６）系ドコサペンタエン酸含有油脂ならびに該油脂の製造方法および用途
WO1997037032A2 (en) 1996-03-28 1997-10-09 Gist-Brocades B.V. Preparation of microbial polyunsaturated fatty acid containing oil from pasteurised biomass
US6255505B1 (en) 1996-03-28 2001-07-03 Gist-Brocades, B.V. Microbial polyunsaturated fatty acid containing oil from pasteurised biomass
ATE249514T1 (de) 1996-03-28 2003-09-15 Dsm Ip Assets Bv Verfahren zur herstellung von granulärer mikrobieller biomasse und gewinnung wertvoller komponenten aus mikrobieller biomasse
JP2000510513A (ja) 1996-05-15 2000-08-15 ヒスト―ブロカデス・ベスローテン・フェンノートシャップ 低ステロール且つ高トリグリセライドの微生物性油を得るための極性溶媒によるステロール抽出
EP0969086A1 (en) 1996-07-03 2000-01-05 Sagami Chemical Research Center Microorganisms producing docosahexaenoic acid and process for the production of docosahexaenoic acid
EP2213750A1 (en) 1996-07-23 2010-08-04 Nagase ChemteX Corporation Process for preparing docosahexaenoic acid and docosapentaenoic acid
US5951875A (en) 1996-12-20 1999-09-14 Eastman Chemical Company Adsorptive bubble separation methods and systems for dewatering suspensions of microalgae and extracting components therefrom
JPH10179182A (ja) * 1996-12-26 1998-07-07 Kirin Brewery Co Ltd 分散安定剤および分散安定方法
GB9701705D0 (en) 1997-01-28 1997-03-19 Norsk Hydro As Purifying polyunsatured fatty acid glycerides
JP4127860B2 (ja) 1997-03-04 2008-07-30 サントリー株式会社 高度不飽和脂肪酸及びこれを含有する脂質の製造方法
ES2337530T3 (es) 1997-05-02 2010-04-26 Dsm Ip Assets B.V. Aislamiento de cristales de carotenoide a partir de biomasa microbiana.
EP1905309A1 (en) 1997-05-27 2008-04-02 SemBioSys Genetics Inc. Uses of oil bodies
US7585645B2 (en) 1997-05-27 2009-09-08 Sembiosys Genetics Inc. Thioredoxin and thioredoxin reductase containing oil body based products
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
JP3836231B2 (ja) 1997-10-17 2006-10-25 日本化学飼料株式会社 ホタテガイ中腸腺から得られる高度不飽和脂肪酸含有油及びその製造方法
DE69823500T2 (de) 1997-11-12 2005-01-20 Mitsubishi Denki K.K. Elektrolumineszentes licht
WO1999032604A1 (en) 1997-12-23 1999-07-01 Dcv, Inc. Doing Business As Bio-Technical Resources Linoleate isomerase
EP0953379B1 (en) 1998-05-01 2004-07-21 F. Hoffmann-La Roche Ag Apparatus for simultaneously monitoring reactions taking place in a plurality of reaction vessels
US6127185A (en) 1998-05-05 2000-10-03 University Of Tennessee Research Corporation Instrument and method for measurement of stability of oils
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
JP2000041684A (ja) 1998-07-29 2000-02-15 Daicel Chem Ind Ltd 新規なｄ−アミノアシラーゼおよびその製造方法、並びに該ｄ−アミノアシラーゼを利用したｄ−アミノ酸の製造方法
JP2000135096A (ja) 1998-08-28 2000-05-16 Tadayuki Imanaka 界面活性剤、その生産方法およびその利用方法
FR2782921B1 (fr) 1998-09-09 2002-09-20 Dior Christian Parfums Extrait lipidique de l'algue skeletonema, procede de preparation et utilisation dans des domaines cosmetique et pharmaceutique, notamment dermatologique
US6166231A (en) 1998-12-15 2000-12-26 Martek Biosciences Corporation Two phase extraction of oil from biomass
CA2260397A1 (en) 1999-01-29 2000-07-29 Atlantis Marine Inc. Method of converting rendered triglyceride oil from marine sources into bland, stable food oil
JP2000245492A (ja) 1999-03-02 2000-09-12 Kyowa Hakko Kogyo Co Ltd 微生物抽出脂質
KR19990046733A (ko) 1999-04-20 1999-07-05 류성구 미생물슈도모나스에의한도코사핵사노인산(ｄｈａ)의제조방법
US6344349B1 (en) 1999-12-06 2002-02-05 Decant Technologies Llc Process and system for electrical extraction of intracellular matter from biological matter
EP2295595B1 (en) 2000-01-19 2019-05-01 DSM IP Assets B.V. Solventless extraction process
CN101519678B (zh) 2000-01-28 2013-11-13 Dsmip资产公司 通过在发酵罐中高密度培养真核微生物来增加含有多烯脂肪酸的脂质的产生
PT1272049E (pt) 2000-04-12 2010-02-15 Gea Westfalia Separator Gmbh Método para fraccionamento de matérias-primas nativas contendo óleo e lípidos polares utilizando álcool e centrifugação
DE10018213A1 (de) 2000-04-12 2001-10-25 Westfalia Separator Ind Gmbh Verfahren zur Fraktionierung von öl-und lecithinhaltigen nativen Rohstoffen
EP1178118A1 (en) 2000-08-02 2002-02-06 Dsm N.V. Isolation of microbial oils
EP1178103A1 (en) 2000-08-02 2002-02-06 Dsm N.V. Purifying crude pufa oils
US20060060520A1 (en) 2001-06-25 2006-03-23 Bomberger David C Systems and methods using a solvent for the removal of lipids from fluids
AU2002323409A1 (en) * 2001-08-24 2003-03-10 Martek Biosciences Boulder Corporation Products containing highly unsaturated fatty acids for use by women and their children during stages of preconception, pregnancy and lactation/post-partum
EP2261312A1 (en) * 2001-12-12 2010-12-15 Martek Biosciences Corporation Extraction and Winterization of Lipids from Oilseed and Microbial Sources
CN1177024C (zh) 2002-03-27 2004-11-24 西南农业大学 水剂法生产食用菜籽蛋白和油脂的方法
ES2774656T3 (es) 2002-05-03 2020-07-22 Dsm Ip Assets Bv Métodos para producir lípidos de alta calidad mediante liberación enzimática desde biomasa
KR20110017017A (ko) 2002-06-19 2011-02-18 디에스엠 아이피 어셋츠 비.브이. 미생물 세포와 미생물 오일용 파스퇴르살균 방법
JP4092193B2 (ja) 2002-12-27 2008-05-28 中国電力株式会社 木質材料からの有害酸の溶出を抑える植生基盤造成方法
CA2801065C (en) 2003-10-02 2020-04-28 Dsm Ip Assets B.V. Production of high levels of dha in microalgae using modified amounts of chloride and potassium
EP2251429B1 (en) 2003-12-30 2017-03-01 DSM IP Assets B.V. Deaeration process
US7038559B2 (en) 2004-02-23 2006-05-02 Ruby Richard C Vertically separated acoustic filters and resonators
WO2005083101A1 (ja) 2004-03-01 2005-09-09 Suntory Limited 長鎖高度不飽和脂肪酸を構成要素として含むリン脂質の製造方法、およびその利用
JP2007534335A (ja) 2004-04-27 2007-11-29 バクスター・インターナショナル・インコーポレイテッド 攪拌タンク反応器システム
EP1597976B1 (en) 2004-05-21 2013-01-30 Nestec S.A. Use of polyol esters of fatty acids in aerated frozen confection with improved nutritional attributes
WO2006046943A2 (en) 2004-10-22 2006-05-04 Martek Biosciences Corporation Methods for producing lipids by liberation from biomass
DE102004062141A1 (de) 2004-12-23 2006-07-06 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls
DE102005003624A1 (de) 2005-01-26 2006-07-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Herstellung und Anwendung eines antioxidativ wirksamen Extraktes aus Crypthecodinium sp.
WO2006128244A1 (en) 2005-06-03 2006-12-07 Mc Farlane Marketing (Aust.) Pty. Ltd. Lipid extract of mussels and method for preparation thereof
CN104745486B (zh) 2005-06-07 2018-01-16 帝斯曼营养品股份公司 生产脂质和抗氧化剂的真核微生物
JP4819888B2 (ja) * 2005-07-01 2011-11-24 マーテック バイオサイエンシーズ コーポレーション 多価不飽和脂肪酸含有油産物ならびにその使用および産生
US7527734B1 (en) 2005-11-15 2009-05-05 Shepherd Samuel L Rapid non-equilibrium decompression of microorganism-containing waste streams
WO2007091070A1 (en) 2006-02-07 2007-08-16 Universitetet I Oslo Omega 3
AU2007270135B9 (en) 2006-07-05 2013-06-27 Fermentalg Production of ultrapure EPA and polar lipids from largely heterotrophic culture
EP1887011A1 (en) 2006-08-09 2008-02-13 Thermphos Trading GmbH Alpha amino acid phosphonic acid compounds, method of preparation and use thereof
EP2074214A2 (en) 2006-09-28 2009-07-01 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
KR100810314B1 (ko) 2006-10-11 2008-03-04 삼성전자주식회사 휴대 단말기의 키 입력 장치
BRPI0720852A2 (pt) 2006-12-22 2014-03-11 Danisco Us Inc Genecor Division Desemulsificação auxiliada por enzima de extratos de lipídio aquosos
CN101224022B (zh) * 2007-01-15 2012-10-31 天津科技大学 水酶法同时制备芝麻油和蛋白质的工艺方法
JP2010530741A (ja) 2007-06-14 2010-09-16 ミトロプーロス，ニコラオス バイオ燃料用の藻育成
WO2008155410A1 (en) 2007-06-21 2008-12-24 Novozymes A/S Production of lipids containing poly-unsaturated fatty acids
US20100226977A1 (en) 2007-08-29 2010-09-09 Aker Biomarine Asa Low viscosity phospholipid compositions
EP2190298B1 (en) 2007-08-29 2015-07-01 Aker Biomarine Antarctic AS A new method for making krill meal
US7981647B2 (en) 2008-03-03 2011-07-19 Joule Unlimited, Inc. Engineered CO2 fixing microorganisms producing carbon-based products of interest
EP2243181B1 (en) 2008-03-26 2015-05-06 Byd Company Limited Cathode materials for lithium batteries
ITMI20081203A1 (it) 2008-06-30 2010-01-01 Eni Spa Procedimento per l'estrazione di acidi grassi da biomassa algale
EP2145942A1 (de) 2008-07-15 2010-01-20 Lonza Ltd. Verfahren zur Isolierung von Ölen aus Zellen und Biomasse
US20120036767A1 (en) 2008-08-04 2012-02-16 Larach Mario C Continuous cultivation, harvesting, and extraction of photosynthetic cultures
PL3196313T3 (pl) 2008-10-02 2021-07-19 Nieves Gonzalez Ramon Ekstrakt z mikroalg zawierający wielonienasycone kwasy tłuszczowe omega-3 i sposób ekstrakcji oleju z mikroorganizmów
US9896642B2 (en) 2008-10-14 2018-02-20 Corbion Biotech, Inc. Methods of microbial oil extraction and separation
MX352984B (es) 2008-10-14 2017-12-15 Terravia Holdings Inc Composiciones alimenticias a partir de biomasa de microalgas.
CN101455240B (zh) * 2008-12-29 2011-04-06 东北农业大学 水酶法提取南瓜籽油的方法
SE534278C2 (sv) 2009-02-17 2011-06-28 Alfa Laval Corp Ab Ett kontinuerligt förfarande för isolering av oljor från alger eller mikroorganismer
US9296985B2 (en) 2009-03-10 2016-03-29 Valicor, Inc. Algae biomass fractionation
KR20120022718A (ko) 2009-03-10 2012-03-12 에스알에스 에너지 조류 바이오매스 분별처리
US8207363B2 (en) * 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
MX2011009750A (es) 2009-03-19 2011-09-29 Martek Biosciences Corp Traustoquitridos, composiciones de acidos grasos y metodos de produccion y usos de los mismos.
US8476060B2 (en) 2009-04-13 2013-07-02 Board Of Regents, The University Of Texas System Process for separating lipids from a biomass
EP2419520B1 (en) 2009-04-14 2019-06-26 Corbion Biotech, Inc. Methods of microbial oil extraction and separation
CN101531690B (zh) 2009-04-28 2011-09-14 浙江神茗山茶业科技有限公司 一种用水作溶剂直接从茶叶籽仁中提取茶皂素和茶叶籽油的工艺
BRPI1012026A2 (pt) 2009-05-26 2018-07-17 Solazyme Inc fracionamento de biomassa microbiana contendo óleo
WO2011059745A1 (en) 2009-10-28 2011-05-19 The Arizona Board Of Regents For And On Behalf Of Arizona State University Bacterium for production of fatty acids
NZ601757A (en) 2010-01-19 2015-01-30 Dsm Ip Assets Bv Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
EP2558565A4 (en) 2010-04-14 2014-01-29 Solazyme Inc FUEL AND CHEMICAL PRODUCTION OF OIL-HOLDING YEAST
US20110256268A1 (en) 2010-04-14 2011-10-20 Solazyme, Inc. Oleaginous Yeast Food Compositions
EP2561049A4 (en) 2010-04-20 2015-03-11 Origin Oil Inc SYSTEMS, APPARATUSES AND METHOD FOR EXTRACTION OF NONPOLAR LIPIDS FROM AN AQUEOUS ALGAE SLEEP AND LIPIDS PRODUCED THEREOF
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
EP2576801B1 (en) 2010-06-01 2019-10-02 DSM IP Assets B.V. Extraction of lipid from cells and products therefrom
US9023625B2 (en) 2010-06-14 2015-05-05 Io-Mega Holding Corporation Methods for production of algae derived oils
WO2012015831A1 (en) 2010-07-26 2012-02-02 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds from biomass
US9028696B2 (en) 2010-07-26 2015-05-12 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds from biomass
BR112013002972A2 (pt) 2010-08-06 2016-06-07 Icm Inc método, bio-óleo, e, sistema para recuperar bio-óleo
US20120040428A1 (en) 2010-08-13 2012-02-16 Paul Reep Procedure for extracting of lipids from algae without cell sacrifice
US20120129244A1 (en) 2010-10-17 2012-05-24 Michael Phillip Green Systems, methods and apparatuses for dewatering, flocculating and harvesting algae cells
CN101985637B (zh) 2010-11-02 2014-05-07 嘉必优生物工程（武汉）有限公司 一种微生物油脂的提取方法
MX354145B (es) 2010-11-03 2018-02-14 Terravia Holdings Inc Aceites microbianos con puntos de fluidez reducidos, fluidos dielectricos producidos a partir de estos y metodos relacionados.
ES2490619T3 (es) 2010-11-08 2014-09-04 Neste Oil Oyj Método de extracción de lípidos a partir de biomasa
WO2012109642A1 (en) 2011-02-12 2012-08-16 Phycal, Inc. Aqueous extraction methods for high lipid microorganisms
CN107630017A (zh) 2011-03-07 2018-01-26 Dsm营养产品股份公司 工程化破囊壶菌属微生物
US20120238732A1 (en) 2011-03-15 2012-09-20 Iowa State University Research Foundation, Inc. Oil extraction from microalgae
FR2975705B1 (fr) 2011-05-27 2014-12-26 Roquette Freres Procede d'extraction du squalene a partir de microalgues
CN102433215A (zh) 2011-09-22 2012-05-02 厦门汇盛生物有限公司 一种从真菌或藻类中物理破壁提取油脂的方法
CN102388988B (zh) 2011-11-08 2013-01-23 中国农业科学院油料作物研究所 一种微生物油的分提方法
US9200236B2 (en) 2011-11-17 2015-12-01 Heliae Development, Llc Omega 7 rich compositions and methods of isolating omega 7 fatty acids
CA2869358C (fr) 2012-04-16 2020-12-08 Roquette Freres Procede de raffinage du squalene produit par microalgues
CN104508113A (zh) 2012-06-29 2015-04-08 Bp生物燃料英国有限公司 从微生物中分离可再生物质的方法
EP3083909A1 (en) 2013-12-20 2016-10-26 DSM IP Assets B.V. Process for extracting lipids for use in production of biofuels
AU2014369046B2 (en) 2013-12-20 2019-02-28 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
SG11201605052XA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
CA2934490C (en) 2013-12-20 2022-05-03 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
CN105829539B (zh) 2013-12-20 2023-11-10 帝斯曼营养品股份公司 从微生物中回收油的方法
ES2984970T3 (es) 2013-12-20 2024-10-31 Mara Renewables Corp Métodos para recuperar aceite de microorganismos
MX370606B (es) 2013-12-20 2019-12-18 Dsm Ip Assets Bv Procedimientos para obtener aceite microbiano a partir de células microbianas.
TWI646189B (zh) 2013-12-20 2019-01-01 荷蘭商Ｄｓｍ智慧財產有限公司 用於從微生物細胞獲得微生物油之方法（五）
CA2954203C (en) 2014-07-07 2023-12-05 Commonwealth Scientific And Industrial Research Organisation Processes for producing industrial products from plant lipids

Also Published As

Publication number Publication date
ES2758782T3 (es) 2020-05-06
CA2801011C (en) 2019-02-26
AU2011261455B2 (en) 2016-03-24
CA2801011A1 (en) 2011-12-08
JP2013532964A (ja) 2013-08-22
US20180142183A1 (en) 2018-05-24
AU2016201321A1 (en) 2016-03-17
US10385289B2 (en) 2019-08-20
AU2017216531B2 (en) 2019-04-11
JP5911479B2 (ja) 2016-04-27
AU2017216531A1 (en) 2017-09-07
EP2576801A2 (en) 2013-04-10
CN103124791A (zh) 2013-05-29
JP6266681B2 (ja) 2018-01-24
WO2011153246A2 (en) 2011-12-08
US10392578B2 (en) 2019-08-27
EP3617318A1 (en) 2020-03-04
US20110295028A1 (en) 2011-12-01
CN103124791B (zh) 2016-08-24
EP2576801A4 (en) 2013-12-18
US20190338214A1 (en) 2019-11-07
EP2576801B1 (en) 2019-10-02
WO2011153246A3 (en) 2012-04-19
AU2011261455A1 (en) 2012-12-20
AR081558A1 (es) 2012-10-03
JP2016163579A (ja) 2016-09-08
AU2019204858A1 (en) 2019-07-25
KR20130108103A (ko) 2013-10-02

Similar Documents

Publication Publication Date Title
KR101961958B1 (ko) 세포로부터의 지질의 추출 및 이로부터의 생성물
KR102065526B1 (ko) 유동점이 낮은 미생물 오일, 이 오일로 제조된 유전성 유체 및 관련 방법
US6166231A (en) Two phase extraction of oil from biomass
CN100460513C (zh) 无溶剂的提取方法
Hoang et al. Extraction of squalene as value-added product from the residual biomass of Schizochytrium mangrovei PQ6 during biodiesel producing process
WO2014052357A1 (en) Methods for extraction of lipids from wet algal biomass
CN113544244B (zh) 通过酶和ph冲击从微生物细胞组合物中获得脂质的方法
RU2841716C2 (ru) Способ извлечения биомасла с улучшенной степенью извлечения масла с использованием процесса охлаждения
TW201200591A (en) Extraction of lipid from cells and products therefrom
CN113574045A (zh) 从微生物细胞组合物中获得脂质的方法
BR112021016754B1 (pt) Métodos de obtenção de lipídios a partir de uma composição de células microbianas
VARDAR OPTIMIZATION OF CULTIVATION CONDITIONS AND ENGINEERING BY RANDOM MUTAGENESIS FOR HIGH LIPID PRODUCTION IN SCHIZOCHYTRIUM SP. S31

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20121227

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20160531

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20170316

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20170927

Patent event code: PE09021S01D

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

Comment text: Final Notice of Reason for Refusal

Patent event date: 20180723

Patent event code: PE09021S02D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20190107

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20190319

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20190319

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20220127

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20230130

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 20240110

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 20250106

Start annual number: 7

End annual number: 7