ES2758782T3 - Extracción de lípido de células y productos del mismo - Google Patents
Extracción de lípido de células y productos del mismo Download PDFInfo
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- ES2758782T3 ES2758782T3 ES11790339T ES11790339T ES2758782T3 ES 2758782 T3 ES2758782 T3 ES 2758782T3 ES 11790339 T ES11790339 T ES 11790339T ES 11790339 T ES11790339 T ES 11790339T ES 2758782 T3 ES2758782 T3 ES 2758782T3
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Mechanical Engineering (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Edible Oils And Fats (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35036310P | 2010-06-01 | 2010-06-01 | |
| US37892310P | 2010-08-31 | 2010-08-31 | |
| US201161452721P | 2011-03-15 | 2011-03-15 | |
| PCT/US2011/038768 WO2011153246A2 (en) | 2010-06-01 | 2011-06-01 | Extraction of lipid from cells and products therefrom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2758782T3 true ES2758782T3 (es) | 2020-05-06 |
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|---|---|---|---|
| ES11790339T Active ES2758782T3 (es) | 2010-06-01 | 2011-06-01 | Extracción de lípido de células y productos del mismo |
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| Country | Link |
|---|---|
| US (3) | US10392578B2 (enExample) |
| EP (2) | EP2576801B1 (enExample) |
| JP (2) | JP5911479B2 (enExample) |
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| CN (1) | CN103124791B (enExample) |
| AR (1) | AR081558A1 (enExample) |
| AU (4) | AU2011261455B2 (enExample) |
| CA (1) | CA2801011C (enExample) |
| ES (1) | ES2758782T3 (enExample) |
| WO (1) | WO2011153246A2 (enExample) |
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| KR20100051131A (ko) | 2000-01-19 | 2010-05-14 | 마텍 바이오싸이언스스 코포레이션 | 무용매 추출 방법 |
| BR122015020119B8 (pt) | 2010-01-19 | 2021-05-04 | Dsm Ip Assets Bv | óleo microbiano, produtos alimentícios para um animal não humano e biomassa isolada |
| AU2011261455B2 (en) | 2010-06-01 | 2016-03-24 | Dsm Ip Assets B.V. | Extraction of lipid from cells and products therefrom |
| US9028696B2 (en) * | 2010-07-26 | 2015-05-12 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds from biomass |
| FR2975705B1 (fr) * | 2011-05-27 | 2014-12-26 | Roquette Freres | Procede d'extraction du squalene a partir de microalgues |
| EP2952183A1 (en) | 2011-07-21 | 2015-12-09 | DSM IP Assets B.V. | Fatty acid compositions |
| JP2014530614A (ja) * | 2011-10-20 | 2014-11-20 | フリンダーズユニバーシティ オブ サウス オーストラリアFlinders University of South Australia | 微細藻類の抽出 |
| MY196019A (en) | 2011-11-01 | 2023-03-07 | Dsm Ip Assets Bv | Oxidatively Stable Polyunsaturated Fatty Acid Containing Oil |
| JP6220388B2 (ja) * | 2012-05-25 | 2017-10-25 | ヘルス・ダイアグノスティック・ラボラトリー，インコーポレーテッド | ステロール／スタノール又はそれらの誘導体の迅速でハイスループットの分析 |
| US8980590B2 (en) | 2012-06-29 | 2015-03-17 | BP Biofuels UK Limited | Process for separation of renewable materials from microorganisms |
| CN106029624B (zh) * | 2013-12-20 | 2021-12-07 | 帝斯曼知识产权资产管理有限公司 | 用于从微生物细胞获得微生物油的方法 |
| EP3082794B1 (en) * | 2013-12-20 | 2024-05-08 | DSM IP Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| CA2934490C (en) * | 2013-12-20 | 2022-05-03 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| EP4223861A3 (en) * | 2013-12-20 | 2023-11-01 | Mara Renewables Corporation | Methods of recovering oil from microorganisms |
| AU2014369042B2 (en) * | 2013-12-20 | 2020-04-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| CN105829539B (zh) * | 2013-12-20 | 2023-11-10 | 帝斯曼营养品股份公司 | 从微生物中回收油的方法 |
| US20160355749A1 (en) * | 2013-12-20 | 2016-12-08 | Dsm Ip Assets B.V. | Process for extracting lipids for use in production of biofuels |
| MX388703B (es) * | 2013-12-20 | 2025-03-20 | Dsm Ip Assets Bv | Procedimientos para obtener aceite microbiano a partir de células microbianas. |
| WO2015123664A1 (en) * | 2014-02-17 | 2015-08-20 | University Of Maryland, Baltimore | Method of extracting lipids from microbes |
| US10494653B2 (en) * | 2014-02-28 | 2019-12-03 | Delft Advanced Biofuels B.V. | Process for the recovery of lipids or hydrocarbons |
| CN105567715B (zh) * | 2014-10-17 | 2021-06-29 | 丰益(上海)生物技术研发中心有限公司 | 裂殖壶菌α-微管蛋白相关序列及其应用 |
| CN107406867B (zh) * | 2015-01-12 | 2021-06-01 | 斯泰潘公司 | 鼠李糖脂组合物的制备 |
| CN106554273B (zh) * | 2015-09-30 | 2020-02-14 | 中国石油化工股份有限公司 | 一种发酵液中长链二羧酸的提纯方法 |
| IL245059A0 (en) * | 2016-04-12 | 2016-06-30 | Technion Res & Dev Foundation | Electrospun scaffolds from extracellular matrix |
| CN109477079A (zh) * | 2016-05-12 | 2019-03-15 | 帝斯曼知识产权资产管理有限公司 | 增加微藻中ω-3多不饱和脂肪酸产量的方法 |
| JP6738214B2 (ja) * | 2016-06-17 | 2020-08-12 | アースリサイクル株式会社 | バイオ燃料の製造方法 |
| CN107523417A (zh) * | 2016-06-21 | 2017-12-29 | 嘉必优生物技术（武汉）股份有限公司 | 提取微生物油脂的方法 |
| JP6998935B2 (ja) * | 2016-07-13 | 2022-01-18 | エボニック オペレーションズ ゲーエムベーハー | 溶解された脂質含有バイオマスから脂質を分離する方法 |
| AU2017297752B2 (en) * | 2016-07-13 | 2021-09-23 | Dsm Ip Assets B.V. | Method for isolating lipids from lipid-containing cells |
| JP6998934B2 (ja) * | 2016-07-13 | 2022-01-18 | エボニック オペレーションズ ゲーエムベーハー | 脂質含有細胞から脂質を単離する方法 |
| US20190249108A1 (en) * | 2016-07-13 | 2019-08-15 | Stephen Robert Cherinko | Method for extracting a microbial oil comprising polyunsaturated fatty acids from a fermentation broth containing oleaginous microorganisms |
| CA3030471C (en) * | 2016-07-13 | 2023-06-20 | Evonik Degussa Gmbh | Method of separating lipids from a lysed lipids containing biomass |
| WO2018050255A1 (en) | 2016-09-19 | 2018-03-22 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Puck and method for manufacturing a puck |
| WO2018112165A1 (en) * | 2016-12-14 | 2018-06-21 | University Of Maryland, Baltimore | Methods for lipid extraction and identification of microbes using same via mass spectrometry |
| EP3562925B1 (en) | 2016-12-27 | 2021-03-10 | Evonik Operations GmbH | Method of isolating lipids from a lipids containing biomass |
| JP6935945B2 (ja) * | 2016-12-29 | 2021-09-15 | ショアライン バイオミー エルエルシー | 細胞を完全に溶解するための併用式溶解プロトコル |
| US11149246B2 (en) | 2016-12-29 | 2021-10-19 | Shoreline Biome, Llc | Methods for cell lysis and preparation of high molecular weight DNA from modified cells |
| US11103808B2 (en) | 2017-08-07 | 2021-08-31 | Amyris, Inc. | Process for recovering isoprenoids produced by microorganisms |
| WO2019034354A1 (en) | 2017-08-17 | 2019-02-21 | Evonik Degussa Gmbh | ENHANCED PRODUCTION OF LIPIDS BY LIMITING AT LEAST TWO SOURCES OF NUTRIENT LIMITING |
| US11814665B2 (en) | 2017-08-17 | 2023-11-14 | Evonik Operations Gmbh | Enhanced production of lipids by limitation of at least two limiting nutrient sources |
| EP3470502A1 (en) | 2017-10-13 | 2019-04-17 | Evonik Degussa GmbH | Method of separating lipids from a lysed lipids containing biomass |
| WO2019121752A1 (en) * | 2017-12-20 | 2019-06-27 | Evonik Degussa Gmbh | Method of isolating lipids from a lipids containing biomass |
| EP3527664A1 (en) * | 2018-02-15 | 2019-08-21 | Evonik Degussa GmbH | Method of isolating lipids from a lipids containing biomass |
| BR112020012339A2 (pt) * | 2017-12-22 | 2020-11-24 | Dsm Ip Assets B.V. | óleo que compreende pelo menmos um ácido graxo poli-insaturado que tem pelo menos 20 átomos de carbono (lc-pufa) |
| WO2019191544A1 (en) * | 2018-03-30 | 2019-10-03 | Dsm Ip Assets B.V. | Method of obtaining a microbial oil and a method of reducing emulsion by maintaining a low concentration of carbohydrate |
| WO2019191545A1 (en) * | 2018-03-30 | 2019-10-03 | Dsm Ip Assets B.V. | Method of reducing emulsion by broth washing |
| CA3101855C (en) * | 2018-05-15 | 2023-06-20 | Evonik Operations Gmbh | Method of isolating lipids from a lipids containing biomass with aid of hydrophobic silica |
| BR112020023222A2 (pt) | 2018-05-15 | 2021-03-23 | Evonik Operations Gmbh | método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão |
| WO2020016363A1 (en) | 2018-07-20 | 2020-01-23 | Total Raffinage Chimie | Wet process for recovering oil produced by microorganism |
| FR3085962B1 (fr) * | 2018-09-14 | 2021-06-18 | Fermentalg | Procede d'extracton d'une huile riche en pufa |
| FR3085825B1 (fr) | 2018-09-14 | 2021-07-16 | Fermentalg | Huile de microorganismes riche en acide docosahexaenoique |
| WO2020094751A1 (de) | 2018-11-09 | 2020-05-14 | Evonik Operations Gmbh | Verfahren zur herstellung einer leicht aufschliessbaren biomasse mit erhöhtem gehalt an polyungesättigten fettsäuren |
| BR112021008842A2 (pt) | 2018-11-09 | 2021-08-17 | Evonik Operations Gmbh | método para produzir uma biomassa com um teor aumentado de ácidos graxos poli-insaturados |
| US20220154098A1 (en) * | 2019-03-14 | 2022-05-19 | Dsm Ip Assets B.V. | Methods of obtaining lipids from a microbial cell composition by enzyme and ph shock |
| EP3938339A4 (en) * | 2019-03-14 | 2022-11-16 | DSM IP Assets B.V. | PROCEDURE FOR RECOVERING LIPIDS FROM A MICROBIAL CELL COMPOSITION |
| CN110106019A (zh) * | 2019-05-20 | 2019-08-09 | 无限极（中国）有限公司 | 一种利用裂壶藻生产复合型多不饱和脂肪酸油脂的方法 |
| CN110172373B (zh) * | 2019-06-14 | 2022-08-05 | 中国农业科学院农产品加工研究所 | 白藜芦醇在花生油中的应用 |
| CN110846346B (zh) * | 2019-11-26 | 2021-06-22 | 瞿瀚鹏 | 富含Sn-2位DHA的微生物油脂及其制备方法和应用 |
| BR112022019834A2 (pt) | 2020-04-03 | 2022-12-06 | Mara Renewables Corp | Óleo microbiano, e, método de compor uma biomassa |
| FR3111912B1 (fr) | 2020-06-24 | 2025-10-03 | Fermentalg | Procédé de culture de microorganismes pour l’accumulation de lipides |
| CN112607826B (zh) * | 2021-03-02 | 2021-05-07 | 烟台垚磊信息技术有限公司 | 一种含油乳化废水处理装置 |
| FR3130842B1 (fr) | 2021-12-22 | 2025-06-27 | CarbonWorks | Procede de captation des phytotoxines dans un reacteur biologique |
| KR102795205B1 (ko) * | 2022-01-27 | 2025-04-10 | 씨제이제일제당 (주) | 냉각 공정을 이용한 오일 회수율이 향상된 바이오오일 추출 방법 |
| WO2023214072A2 (en) | 2022-05-05 | 2023-11-09 | Dsm Ip Assets B.V. | Novel process |
Family Cites Families (161)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2753362A (en) | 1951-05-18 | 1956-07-03 | Standard Brands Inc | Process of extracting lipids from plant and animal tissue |
| GB808128A (en) | 1955-12-01 | 1959-01-28 | Nat Res Dev | A method of increasing the fatty contents of such micro-organisms as yeasts, bacteria and moulds |
| US3089821A (en) | 1959-10-28 | 1963-05-14 | Merck & Co Inc | Process for the preparation of lipids |
| DE2056896B2 (de) | 1970-02-18 | 1979-12-06 | Veb Schwermaschinenbau Kombinat Ernst Thaelmann Magdeburg, Ddr 3011 Magdeburg | Verfahren zur gleichzeitigen Gewinnung von Fett und Protein aus pflanzlichen, ölhaltigen Rohstoffen und Zwischenprodukten |
| US3878232A (en) | 1970-09-28 | 1975-04-15 | Staley Mfg Co A E | Extraction process to improve the quality and yield of crude vegetable oils |
| GB1466853A (en) | 1973-05-22 | 1977-03-09 | Simon Rosedowns Ltd | Extraction |
| US4504473A (en) | 1982-06-30 | 1985-03-12 | Ribi Immunochem Research, Inc. | Pyridine soluble extract of a microorganism |
| DE3248167A1 (de) | 1982-12-27 | 1984-06-28 | Wintershall Ag, 3100 Celle | Trehaloselipidtetraester |
| JPS61170397A (ja) | 1985-01-22 | 1986-08-01 | Agency Of Ind Science & Technol | モルテイエレラ属糸状菌体の多段抽出処理方法 |
| US4870011A (en) | 1985-01-22 | 1989-09-26 | Director General Of Agency Of Industrial Science And Technology | Method for obtaining lipids from fungus bodies |
| US4792418A (en) | 1985-08-14 | 1988-12-20 | Century Laboratories, Inc. | Method of extraction and purification of polyunsaturated fatty acids from natural sources |
| JPS6244170A (ja) | 1985-08-19 | 1987-02-26 | Agency Of Ind Science & Technol | モルテイエレラ属糸状菌体の超臨界流体による抽出方法 |
| US4680314A (en) | 1985-08-30 | 1987-07-14 | Microbio Resources, Inc. | Process for producing a naturally-derived carotene/oil composition by direct extraction from algae |
| JPS62278987A (ja) | 1986-05-26 | 1987-12-03 | Kanegafuchi Chem Ind Co Ltd | 酵素反応生成物の回収方法 |
| DK199887D0 (da) | 1987-04-15 | 1987-04-15 | Danisco Bioteknologi As | Gaerstamme |
| JPS63304990A (ja) | 1987-06-04 | 1988-12-13 | Meiji Seika Kaisha Ltd | 菌体内有効成分の抽出方法 |
| FR2621327B1 (fr) | 1987-10-06 | 1990-01-05 | Commissariat Energie Atomique | Procede de production et d'extraction de polysaccharides a partir d'une culture de porphyridium cruentum et dispositif pour la mise en oeuvre du procede |
| JPH0198494A (ja) | 1987-10-09 | 1989-04-17 | Agency Of Ind Science & Technol | バイオリアクター |
| US5340594A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Food product having high concentrations of omega-3 highly unsaturated fatty acids |
| US5130242A (en) | 1988-09-07 | 1992-07-14 | Phycotech, Inc. | Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids |
| US5340742A (en) | 1988-09-07 | 1994-08-23 | Omegatech Inc. | Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids |
| US6451567B1 (en) | 1988-09-07 | 2002-09-17 | Omegatech, Inc. | Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms |
| US5173409A (en) | 1989-12-08 | 1992-12-22 | Ecogen Inc. | Recovery of bt endotoxin protein from lysed cell mixtures |
| US5288619A (en) | 1989-12-18 | 1994-02-22 | Kraft General Foods, Inc. | Enzymatic method for preparing transesterified oils |
| FR2656874B1 (fr) | 1990-01-11 | 1992-04-03 | Commissariat Energie Atomique | Procede de production et d'extraction d'anti-oxydants a partir d'une culture de micro-organismes et photobioreacteur pour la mise en óoeuvre de ce procede. |
| US5407957A (en) | 1990-02-13 | 1995-04-18 | Martek Corporation | Production of docosahexaenoic acid by dinoflagellates |
| US5133963A (en) | 1990-12-21 | 1992-07-28 | Shuntaro Ise | Method of producing commercially useful poultry products with increased concentrations of Omega-3 polyunsaturated fatty acids |
| US5658767A (en) | 1991-01-24 | 1997-08-19 | Martek Corporation | Arachidonic acid and methods for the production and use thereof |
| US6270828B1 (en) | 1993-11-12 | 2001-08-07 | Cargrill Incorporated | Canola variety producing a seed with reduced glucosinolates and linolenic acid yielding an oil with low sulfur, improved sensory characteristics and increased oxidative stability |
| FR2686619B1 (fr) | 1992-01-28 | 1995-07-13 | Commissariat Energie Atomique | Procede de production selective de lipides poly-insatures a partir d'une culture de micro-algues du type porphyridium et cuve utilisee dans ce procede. |
| US5476787A (en) * | 1992-04-24 | 1995-12-19 | Director-General Of Agency Of Industrial Science And Technology | Method of removing nitrogen impurities from water using hydrocarbon-producing microalga |
| DE4219360C2 (de) | 1992-06-12 | 1994-07-28 | Milupa Ag | Verfahren zur Gewinnung von Lipiden mit einem hohen Anteil von langkettig-hochungesättigten Fettsäuren |
| EP0928556A3 (en) | 1993-04-27 | 1999-09-15 | Cargill, Incorporated | Non-hydrogenated canola oil for food applications |
| EP0707487B1 (en) * | 1993-06-09 | 2004-04-14 | Martek Biosciences Corporation | Methods and pharmaceutical compositions useful for treating neurological disorders |
| FR2719222B1 (fr) | 1994-05-02 | 1996-06-21 | Rocher Yves Biolog Vegetale | Vésicules lipidiques, leur procédé de fabrication et leurs applications. |
| WO1996005278A1 (de) | 1994-08-16 | 1996-02-22 | Dr. Frische Gmbh | Verfahren zur gewinnung von nicht wasserlöslichen, nativen produkten aus nativen stoffgemengen mit hilfe der zentrifugalkraft |
| KR100212644B1 (ko) | 1994-10-29 | 1999-08-02 | 토니헬샴 | 유압식 건설기계의 엔진회전수 제어장치 및 제어방법 |
| US5583019A (en) | 1995-01-24 | 1996-12-10 | Omegatech Inc. | Method for production of arachidonic acid |
| JP2764572B2 (ja) | 1995-04-17 | 1998-06-11 | 工業技術院長 | ドコサヘキサエン酸生産能を有する新規微生物及びそれを用いたドコサヘキサエン酸の製造方法 |
| AU5346296A (en) | 1995-04-17 | 1996-11-07 | Japan As Represented By Director-General Of Agency Of Industrial Science And Technology | Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fa tty acids by using the microorganisms |
| ES2130515T3 (es) | 1995-05-04 | 1999-07-01 | Nestle Sa | Procedimiento para fraccionar acidos grasos. |
| JPH099981A (ja) | 1995-06-28 | 1997-01-14 | Sekiyu Sangyo Kasseika Center | 油水二相系微生物反応における油水分離方法 |
| GB9514649D0 (en) | 1995-07-18 | 1995-09-13 | Zeneca Ltd | Extraction of triglycerides from microorganisms |
| JP3985035B2 (ja) | 1995-09-14 | 2007-10-03 | 独立行政法人産業技術総合研究所 | （ｎ−６）系ドコサペンタエン酸含有油脂ならびに該油脂の製造方法および用途 |
| US6255505B1 (en) | 1996-03-28 | 2001-07-03 | Gist-Brocades, B.V. | Microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
| EP1369474A1 (en) | 1996-03-28 | 2003-12-10 | DSM IP Assets B.V. | Process for the preparation of a granular microbial biomass and isolation of valuable compounds therefrom |
| EP1506996A3 (en) | 1996-03-28 | 2006-06-14 | DSM IP Assets B.V. | Preparation of microbial polyunsaturated fatty acid containing oil from pasteurised biomass |
| DK0904339T4 (da) | 1996-05-15 | 2007-01-29 | Dsm Ip Assets Bv | Sterolekstraktion med et polært oplösningsmiddel til opnåelse af lavsterolholdig mikrobiel olie |
| WO1998001536A1 (en) | 1996-07-03 | 1998-01-15 | Sagami Chemical Research Center | Microorganisms producing docosahexaenoic acid and process for the production of docosahexaenoic acid |
| EP1785492B1 (en) | 1996-07-23 | 2010-05-26 | Nagase Chemtex Corporation | Process for preparing docosahexaenoic acid and docosapentaenoic acid |
| US5951875A (en) | 1996-12-20 | 1999-09-14 | Eastman Chemical Company | Adsorptive bubble separation methods and systems for dewatering suspensions of microalgae and extracting components therefrom |
| JPH10179182A (ja) * | 1996-12-26 | 1998-07-07 | Kirin Brewery Co Ltd | 分散安定剤および分散安定方法 |
| GB9701705D0 (en) | 1997-01-28 | 1997-03-19 | Norsk Hydro As | Purifying polyunsatured fatty acid glycerides |
| EP2175031A3 (en) | 1997-03-04 | 2010-07-07 | Suntory Holdings Limited | Process for preparing highly unsaturated fatty acid and lipid containing highly unsaturated fatty acid |
| EP0979302B1 (en) | 1997-05-02 | 2009-12-16 | DSM IP Assets B.V. | Isolation of carotenoid crystals from microbial biomass |
| EP1905309A1 (en) | 1997-05-27 | 2008-04-02 | SemBioSys Genetics Inc. | Uses of oil bodies |
| US7585645B2 (en) | 1997-05-27 | 2009-09-08 | Sembiosys Genetics Inc. | Thioredoxin and thioredoxin reductase containing oil body based products |
| US6566583B1 (en) * | 1997-06-04 | 2003-05-20 | Daniel Facciotti | Schizochytrium PKS genes |
| JP3836231B2 (ja) | 1997-10-17 | 2006-10-25 | 日本化学飼料株式会社 | ホタテガイ中腸腺から得られる高度不飽和脂肪酸含有油及びその製造方法 |
| US6528941B1 (en) | 1997-11-12 | 2003-03-04 | Mitsubishi Denki Kabushiki Kaisha | Electroluminescent device and shield therefor |
| EP1042451A4 (en) | 1997-12-23 | 2001-07-04 | Dcv Inc | LINOLEAT ISOMERASE |
| ES2224347T3 (es) | 1998-05-01 | 2005-03-01 | F. Hoffmann-La Roche Ag | Aparato para controlar simultaneamente las reacciones que tienen lugaren una pluralidad de recipientes de reaccion. |
| AU3786799A (en) | 1998-05-05 | 1999-11-23 | University Of Tennessee Research Corporation, The | An instrument and method for measurement of stability of oils |
| US6291245B1 (en) | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
| JP2000041684A (ja) | 1998-07-29 | 2000-02-15 | Daicel Chem Ind Ltd | 新規なｄ−アミノアシラーゼおよびその製造方法、並びに該ｄ−アミノアシラーゼを利用したｄ−アミノ酸の製造方法 |
| JP2000135096A (ja) | 1998-08-28 | 2000-05-16 | Tadayuki Imanaka | 界面活性剤、その生産方法およびその利用方法 |
| FR2782921B1 (fr) | 1998-09-09 | 2002-09-20 | Dior Christian Parfums | Extrait lipidique de l'algue skeletonema, procede de preparation et utilisation dans des domaines cosmetique et pharmaceutique, notamment dermatologique |
| US6166231A (en) | 1998-12-15 | 2000-12-26 | Martek Biosciences Corporation | Two phase extraction of oil from biomass |
| CA2260397A1 (en) | 1999-01-29 | 2000-07-29 | Atlantis Marine Inc. | Method of converting rendered triglyceride oil from marine sources into bland, stable food oil |
| JP2000245492A (ja) | 1999-03-02 | 2000-09-12 | Kyowa Hakko Kogyo Co Ltd | 微生物抽出脂質 |
| KR19990046733A (ko) | 1999-04-20 | 1999-07-05 | 류성구 | 미생물슈도모나스에의한도코사핵사노인산(ｄｈａ)의제조방법 |
| US6344349B1 (en) | 1999-12-06 | 2002-02-05 | Decant Technologies Llc | Process and system for electrical extraction of intracellular matter from biological matter |
| KR20100051131A (ko) | 2000-01-19 | 2010-05-14 | 마텍 바이오싸이언스스 코포레이션 | 무용매 추출 방법 |
| EP1707055A3 (en) | 2000-01-28 | 2009-01-14 | Martek Biosciences Corporation | Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors |
| WO2001076715A2 (en) | 2000-04-12 | 2001-10-18 | Westfalia Separator Industry Gmbh | Method for the fractionation of oil and polar lipid-containing native raw materials |
| DE10018213A1 (de) | 2000-04-12 | 2001-10-25 | Westfalia Separator Ind Gmbh | Verfahren zur Fraktionierung von öl-und lecithinhaltigen nativen Rohstoffen |
| EP1178118A1 (en) | 2000-08-02 | 2002-02-06 | Dsm N.V. | Isolation of microbial oils |
| EP1178103A1 (en) * | 2000-08-02 | 2002-02-06 | Dsm N.V. | Purifying crude pufa oils |
| US20060060520A1 (en) * | 2001-06-25 | 2006-03-23 | Bomberger David C | Systems and methods using a solvent for the removal of lipids from fluids |
| AU2002323409A1 (en) * | 2001-08-24 | 2003-03-10 | Martek Biosciences Boulder Corporation | Products containing highly unsaturated fatty acids for use by women and their children during stages of preconception, pregnancy and lactation/post-partum |
| WO2003049832A1 (en) * | 2001-12-12 | 2003-06-19 | Martek Biosciences Boulder Corp. | Extraction and winterization of lipids from oilseed and microbial sources |
| CN1177024C (zh) * | 2002-03-27 | 2004-11-24 | 西南农业大学 | 水剂法生产食用菜籽蛋白和油脂的方法 |
| EP2266525B1 (en) * | 2002-05-03 | 2012-07-11 | Martek Biosciences Corporation | High quality lipids and methods for producing by enzymatic liberation from biomass |
| EP2270191A1 (en) | 2002-06-19 | 2011-01-05 | DSM IP Assets B.V. | Preparation of microbial oil containing polyunsaturated fatty acids |
| JP4092193B2 (ja) | 2002-12-27 | 2008-05-28 | 中国電力株式会社 | 木質材料からの有害酸の溶出を抑える植生基盤造成方法 |
| ES2454196T3 (es) | 2003-10-02 | 2014-04-09 | Dsm Ip Assets B.V. | Producción de niveles altos de DHA en microalgas utilizando cantidades modificadas de cloruro y potasio |
| EA009568B1 (ru) | 2003-12-30 | 2008-02-28 | ДСМ АйПи ЭССЕТС Б.В. | Способ деаэрации |
| US7038559B2 (en) | 2004-02-23 | 2006-05-02 | Ruby Richard C | Vertically separated acoustic filters and resonators |
| WO2005083101A1 (ja) | 2004-03-01 | 2005-09-09 | Suntory Limited | 長鎖高度不飽和脂肪酸を構成要素として含むリン脂質の製造方法、およびその利用 |
| AU2005240969A1 (en) | 2004-04-27 | 2005-11-17 | Baxter Healthcare S.A. | Stirred-tank reactor system |
| EP1597976B1 (en) | 2004-05-21 | 2013-01-30 | Nestec S.A. | Use of polyol esters of fatty acids in aerated frozen confection with improved nutritional attributes |
| WO2006046943A2 (en) | 2004-10-22 | 2006-05-04 | Martek Biosciences Corporation | Methods for producing lipids by liberation from biomass |
| DE102004062141A1 (de) | 2004-12-23 | 2006-07-06 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls |
| DE102005003624A1 (de) | 2005-01-26 | 2006-07-27 | Nutrinova Nutrition Specialties & Food Ingredients Gmbh | Herstellung und Anwendung eines antioxidativ wirksamen Extraktes aus Crypthecodinium sp. |
| NZ563125A (en) | 2005-06-03 | 2009-11-27 | Mcfarlane Marketing Aust Pty L | Lipid extract of mussels and method for preparation thereof |
| EP2447356B1 (en) | 2005-06-07 | 2016-04-20 | DSM Nutritional Products AG | Eukaryotic microorganisms for producing lipids and antioxidants |
| EP1903883A4 (en) * | 2005-07-01 | 2010-06-23 | Martek Biosciences Corp | OIL PRODUCT CONTAINING MULTIPLE UNSATURATED FATTY ACIDS AND ITS USES AND MANUFACTURING |
| US7527734B1 (en) | 2005-11-15 | 2009-05-05 | Shepherd Samuel L | Rapid non-equilibrium decompression of microorganism-containing waste streams |
| US20090221705A1 (en) | 2006-02-07 | 2009-09-03 | Pal Rongved | Omega 3 |
| CA2656311C (en) | 2006-07-05 | 2016-06-21 | Photonz Corporation Limited | Ultra pure epa and polar lipids produced in largely heterotrophic culture |
| EP1887011A1 (en) | 2006-08-09 | 2008-02-13 | Thermphos Trading GmbH | Alpha amino acid phosphonic acid compounds, method of preparation and use thereof |
| EP2074214A2 (en) | 2006-09-28 | 2009-07-01 | Microbia, Inc. | Production of sterols in oleaginous yeast and fungi |
| KR100810314B1 (ko) | 2006-10-11 | 2008-03-04 | 삼성전자주식회사 | 휴대 단말기의 키 입력 장치 |
| BRPI0720852A2 (pt) * | 2006-12-22 | 2014-03-11 | Danisco Us Inc Genecor Division | Desemulsificação auxiliada por enzima de extratos de lipídio aquosos |
| CN101224022B (zh) | 2007-01-15 | 2012-10-31 | 天津科技大学 | 水酶法同时制备芝麻油和蛋白质的工艺方法 |
| CN101820743A (zh) | 2007-06-14 | 2010-09-01 | 尼古拉斯·米特罗普洛斯 | 用于生物燃料的藻类生长 |
| WO2008155410A1 (en) | 2007-06-21 | 2008-12-24 | Novozymes A/S | Production of lipids containing poly-unsaturated fatty acids |
| US20090061067A1 (en) | 2007-08-29 | 2009-03-05 | Aker Biomarine Asa | Method for making krill meal |
| US20100226977A1 (en) | 2007-08-29 | 2010-09-09 | Aker Biomarine Asa | Low viscosity phospholipid compositions |
| EP2706111A1 (en) | 2008-03-03 | 2014-03-12 | Joule Unlimited Technologies, Inc. | Engineered CO2 fixing microorganisms producing carbon-based products of interest |
| JP5244966B2 (ja) | 2008-03-26 | 2013-07-24 | ビーワイディー カンパニー リミテッド | リチウム電池用正極材料 |
| ITMI20081203A1 (it) | 2008-06-30 | 2010-01-01 | Eni Spa | Procedimento per l'estrazione di acidi grassi da biomassa algale |
| EP2145942A1 (de) | 2008-07-15 | 2010-01-20 | Lonza Ltd. | Verfahren zur Isolierung von Ölen aus Zellen und Biomasse |
| WO2010017243A1 (en) | 2008-08-04 | 2010-02-11 | Kai Bioenergy | Continuous cultivation, harvesting, and oil extraction of photosynthetic cultures |
| CA2739440C (en) | 2008-10-02 | 2018-11-06 | Feyecon B.V. | Microalgae extract containing .omega.3-polyunsaturated fatty acids and method for extracting oil from micro-organisms |
| US9896642B2 (en) | 2008-10-14 | 2018-02-20 | Corbion Biotech, Inc. | Methods of microbial oil extraction and separation |
| AU2009303354C9 (en) | 2008-10-14 | 2017-09-07 | Corbion Biotech, Inc. | Food compositions of microalgal biomass |
| CN101455240B (zh) * | 2008-12-29 | 2011-04-06 | 东北农业大学 | 水酶法提取南瓜籽油的方法 |
| SE534278C2 (sv) | 2009-02-17 | 2011-06-28 | Alfa Laval Corp Ab | Ett kontinuerligt förfarande för isolering av oljor från alger eller mikroorganismer |
| US9296985B2 (en) | 2009-03-10 | 2016-03-29 | Valicor, Inc. | Algae biomass fractionation |
| KR20120022718A (ko) | 2009-03-10 | 2012-03-12 | 에스알에스 에너지 | 조류 바이오매스 분별처리 |
| US8207363B2 (en) * | 2009-03-19 | 2012-06-26 | Martek Biosciences Corporation | Thraustochytrids, fatty acid compositions, and methods of making and uses thereof |
| BRPI0923968B1 (pt) | 2009-03-19 | 2020-04-28 | Dsm Ip Assets Bv | composição, produto alimentício e alimento animal compreendendo óleo microbiano |
| US8476060B2 (en) | 2009-04-13 | 2013-07-02 | Board Of Regents, The University Of Texas System | Process for separating lipids from a biomass |
| BRPI1014451B1 (pt) | 2009-04-14 | 2018-12-18 | Solazyme Inc | métodos para extrair óleos triglicerídeos a partir de biomassa de microalga |
| CN101531690B (zh) * | 2009-04-28 | 2011-09-14 | 浙江神茗山茶业科技有限公司 | 一种用水作溶剂直接从茶叶籽仁中提取茶皂素和茶叶籽油的工艺 |
| WO2010138620A1 (en) | 2009-05-26 | 2010-12-02 | Solazyme, Inc. | Fractionation of oil-bearing microbial biomass |
| WO2011059745A1 (en) | 2009-10-28 | 2011-05-19 | The Arizona Board Of Regents For And On Behalf Of Arizona State University | Bacterium for production of fatty acids |
| BR122015020119B8 (pt) | 2010-01-19 | 2021-05-04 | Dsm Ip Assets Bv | óleo microbiano, produtos alimentícios para um animal não humano e biomassa isolada |
| US20110252696A1 (en) | 2010-04-14 | 2011-10-20 | Solazyme, Inc. | Fuel And Chemical Production From Oleaginous Yeast |
| US20110256268A1 (en) | 2010-04-14 | 2011-10-20 | Solazyme, Inc. | Oleaginous Yeast Food Compositions |
| WO2011133181A1 (en) | 2010-04-20 | 2011-10-27 | Origin Oil, Inc. | Systems, apparatuses, and methods for extracting non-polar lipids from an a aqueous algae slurry and lipids produced therefrom |
| WO2011133610A1 (en) | 2010-04-22 | 2011-10-27 | E. I. Du Pont De Nemours And Company | Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass |
| AU2011261455B2 (en) | 2010-06-01 | 2016-03-24 | Dsm Ip Assets B.V. | Extraction of lipid from cells and products therefrom |
| US9023625B2 (en) | 2010-06-14 | 2015-05-05 | Io-Mega Holding Corporation | Methods for production of algae derived oils |
| BR112013002003A2 (pt) | 2010-07-26 | 2019-09-24 | Sapphire Energy Inc | processo para recuperação de compostos oleaginosos de biomassa |
| US9028696B2 (en) | 2010-07-26 | 2015-05-12 | Sapphire Energy, Inc. | Process for the recovery of oleaginous compounds from biomass |
| BR112013002972A2 (pt) | 2010-08-06 | 2016-06-07 | Icm Inc | método, bio-óleo, e, sistema para recuperar bio-óleo |
| US20120040428A1 (en) | 2010-08-13 | 2012-02-16 | Paul Reep | Procedure for extracting of lipids from algae without cell sacrifice |
| US20120129244A1 (en) | 2010-10-17 | 2012-05-24 | Michael Phillip Green | Systems, methods and apparatuses for dewatering, flocculating and harvesting algae cells |
| CN101985637B (zh) | 2010-11-02 | 2014-05-07 | 嘉必优生物工程（武汉）有限公司 | 一种微生物油脂的提取方法 |
| BR112013011039A8 (pt) | 2010-11-03 | 2017-10-03 | Solazyme Inc | Óleos microbianos com pontos de escorrimento reduzidos, fluidos dielétricos produzidos dos mesmos, e métodos relacionados |
| PT2450425E (pt) | 2010-11-08 | 2014-07-24 | Neste Oil Oyj | Um método para a extração de lípidos a partir de biomassa |
| WO2012109642A1 (en) | 2011-02-12 | 2012-08-16 | Phycal, Inc. | Aqueous extraction methods for high lipid microorganisms |
| MX338479B (es) | 2011-03-07 | 2016-04-19 | Dsm Nutritional Products Ag | Microorganismos thraustochytrid modificados. |
| WO2012125611A2 (en) | 2011-03-15 | 2012-09-20 | Iowa State University Research Foundation | Oil extraction from microalgae |
| FR2975705B1 (fr) | 2011-05-27 | 2014-12-26 | Roquette Freres | Procede d'extraction du squalene a partir de microalgues |
| CN102433215A (zh) | 2011-09-22 | 2012-05-02 | 厦门汇盛生物有限公司 | 一种从真菌或藻类中物理破壁提取油脂的方法 |
| CN102388988B (zh) | 2011-11-08 | 2013-01-23 | 中国农业科学院油料作物研究所 | 一种微生物油的分提方法 |
| US9200236B2 (en) | 2011-11-17 | 2015-12-01 | Heliae Development, Llc | Omega 7 rich compositions and methods of isolating omega 7 fatty acids |
| KR102192410B1 (ko) | 2012-04-16 | 2020-12-17 | 로께뜨프레르 | 미세조류에 의해 생성된 스쿠알렌의 정련방법 |
| US8980590B2 (en) | 2012-06-29 | 2015-03-17 | BP Biofuels UK Limited | Process for separation of renewable materials from microorganisms |
| CN106029624B (zh) | 2013-12-20 | 2021-12-07 | 帝斯曼知识产权资产管理有限公司 | 用于从微生物细胞获得微生物油的方法 |
| US20160355749A1 (en) | 2013-12-20 | 2016-12-08 | Dsm Ip Assets B.V. | Process for extracting lipids for use in production of biofuels |
| CN105829539B (zh) | 2013-12-20 | 2023-11-10 | 帝斯曼营养品股份公司 | 从微生物中回收油的方法 |
| AU2014369042B2 (en) | 2013-12-20 | 2020-04-30 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| CA2934490C (en) | 2013-12-20 | 2022-05-03 | Dsm Ip Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| MX388703B (es) | 2013-12-20 | 2025-03-20 | Dsm Ip Assets Bv | Procedimientos para obtener aceite microbiano a partir de células microbianas. |
| EP3082794B1 (en) | 2013-12-20 | 2024-05-08 | DSM IP Assets B.V. | Processes for obtaining microbial oil from microbial cells |
| EP4223861A3 (en) | 2013-12-20 | 2023-11-01 | Mara Renewables Corporation | Methods of recovering oil from microorganisms |
| BR122020005879B1 (pt) | 2014-07-07 | 2022-09-27 | Commonwealth Scientific And Industrial Research Organisation | Processo para produzir um produto industrial, processo para a produção de lipídeos extraídos, processo para a obtenção de uma célula eucariótica recombinante, método de produção de uma planta, processo para produzir semente, lipídeos recuperados ou extraídos, produto industrial, processo para produzir combustível, processo para produzir combustível diesel sintético, processo para produção de um biocombustível, processo para produzir um ingrediente alimentar, alimentos e processo para a alimentação de um animal |
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- 2011-06-01 CA CA2801011A patent/CA2801011C/en active Active
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| EP2576801B1 (en) | 2019-10-02 |
| JP6266681B2 (ja) | 2018-01-24 |
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| AU2011261455B2 (en) | 2016-03-24 |
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| US10392578B2 (en) | 2019-08-27 |
| AR081558A1 (es) | 2012-10-03 |
| AU2017216531B2 (en) | 2019-04-11 |
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| EP2576801A2 (en) | 2013-04-10 |
| JP5911479B2 (ja) | 2016-04-27 |
| AU2017216531A1 (en) | 2017-09-07 |
| JP2013532964A (ja) | 2013-08-22 |
| EP2576801A4 (en) | 2013-12-18 |
| CA2801011A1 (en) | 2011-12-08 |
| US20190338214A1 (en) | 2019-11-07 |
| JP2016163579A (ja) | 2016-09-08 |
| CN103124791B (zh) | 2016-08-24 |
| WO2011153246A2 (en) | 2011-12-08 |
| CN103124791A (zh) | 2013-05-29 |
| CA2801011C (en) | 2019-02-26 |
| AU2011261455A1 (en) | 2012-12-20 |
| US20110295028A1 (en) | 2011-12-01 |
| AU2016201321A1 (en) | 2016-03-17 |
| WO2011153246A3 (en) | 2012-04-19 |
| US10385289B2 (en) | 2019-08-20 |
| KR20130108103A (ko) | 2013-10-02 |
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