JPH0134599B2 - - Google Patents

Info

Publication number
JPH0134599B2
JPH0134599B2 JP56213193A JP21319381A JPH0134599B2 JP H0134599 B2 JPH0134599 B2 JP H0134599B2 JP 56213193 A JP56213193 A JP 56213193A JP 21319381 A JP21319381 A JP 21319381A JP H0134599 B2 JPH0134599 B2 JP H0134599B2
Authority
JP
Japan
Prior art keywords
plasmid
trp
promoter
escherichia coli
atcc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP56213193A
Other languages
English (en)
Japanese (ja)
Other versions
JPS58110600A (ja
Inventor
Seiga Ito
Tatsuya Nishi
Tamio Mizukami
Tadashi Matsumoto
Tetsuo Oka
Koreaki Taniguchi
Haruo Sugano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Priority to JP56213193A priority Critical patent/JPS58110600A/ja
Priority to DE8282111895T priority patent/DE3277307D1/de
Priority to US06/452,290 priority patent/US4686191A/en
Priority to EP82111895A priority patent/EP0083069B1/en
Priority to CA000418366A priority patent/CA1211059A/en
Publication of JPS58110600A publication Critical patent/JPS58110600A/ja
Publication of JPH0134599B2 publication Critical patent/JPH0134599B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/14—Lymphokine; related peptides
    • Y10S930/142—Interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
JP56213193A 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド Granted JPS58110600A (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP56213193A JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
DE8282111895T DE3277307D1 (en) 1981-12-25 1982-12-22 Recombinant plasmid containing human interferon-beta gene
US06/452,290 US4686191A (en) 1981-12-25 1982-12-22 Recombinant plasmid containing human interferon-beta gene
EP82111895A EP0083069B1 (en) 1981-12-25 1982-12-22 Recombinant plasmid containing human interferon-beta gene
CA000418366A CA1211059A (en) 1981-12-25 1982-12-22 RECOMBINANT PLASMID CONTAINING HUMAN INTERFERON-.beta. GENE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56213193A JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド

Publications (2)

Publication Number Publication Date
JPS58110600A JPS58110600A (ja) 1983-07-01
JPH0134599B2 true JPH0134599B2 (enExample) 1989-07-20

Family

ID=16635072

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56213193A Granted JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド

Country Status (5)

Country Link
US (1) US4686191A (enExample)
EP (1) EP0083069B1 (enExample)
JP (1) JPS58110600A (enExample)
CA (1) CA1211059A (enExample)
DE (1) DE3277307D1 (enExample)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58141796A (ja) * 1982-02-18 1983-08-23 Kyowa Hakko Kogyo Co Ltd ペプチドの製造法
AU575301B2 (en) * 1982-10-08 1988-07-28 Juridical Foundation, Japanese Foundation For Cancer Research Novel vector
JPH0740925B2 (ja) * 1983-03-14 1995-05-10 協和醗酵工業株式会社 新規ヒトインタ−フエロン−γポリペプチド
JPS59203495A (ja) * 1983-04-28 1984-11-17 Kyowa Hakko Kogyo Co Ltd 外来遺伝子の新規な発現方法
EP0152483B1 (en) * 1983-08-22 1989-03-29 Kyowa Hakko Kogyo Co., Ltd. Process for producing peptide
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
EP0165613B1 (en) * 1984-06-22 1992-01-15 Hitachi, Ltd. Process for controlling culture of recombinants
FR2579224B1 (fr) * 1985-03-25 1987-05-22 Genetica Procede de preparation microbiologique de la serum-albumine humaine
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
DK174044B1 (da) 1986-12-23 2002-05-06 Kyowa Hakko Kogyo Kk Polypeptid afledt fra human granulocytkolonistimulerende faktor, og fremgangsmåde til fremstilling deraf, DNA kodende for nævnte polypeptid, rekombinant plasmid indeholdende nævnte DNA, og mikroorganismer indeholdende nævnte rekombinante plasmid.......
DE4128319A1 (de) * 1991-08-27 1993-03-04 Bioferon Biochem Substanz Neues rekombinantes human-ifn-beta, verfahren zu dessen herstellung und dieses enthaltende pharmazeutische zubereitungen
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
CN1295336C (zh) 1998-04-14 2007-01-17 协和发酵工业株式会社 利用微生物生产类异戊二烯化合物的方法和检测具有抗菌或除草活性的化合物的方法
AU3956399A (en) 1998-06-02 1999-12-20 Kyowa Hakko Kogyo Co. Ltd. Iga nephropathy-associated dna
US7431921B2 (en) 1999-08-27 2008-10-07 Maxygen Aps Interferon beta-like molecules
US6531122B1 (en) 1999-08-27 2003-03-11 Maxygen Aps Interferon-β variants and conjugates
US7144574B2 (en) * 1999-08-27 2006-12-05 Maxygen Aps Interferon β variants and conjugates
US7071160B2 (en) 2000-06-15 2006-07-04 Kyowa Hakko Kogyo Co., Ltd. Insulin-like growth factor-binding protein
FR2810674A1 (fr) * 2000-06-22 2001-12-28 Pf Medicament Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
US20020169290A1 (en) * 2000-11-02 2002-11-14 Claus Bornaes New multimeric interferon beta polypeptides
EP1371727A4 (en) * 2001-02-22 2004-07-28 Gencom Corp RECOMBINANT GENE CONTAINING A REVERSE REPETITION SEQUENCE AND USE THEREOF
CZ20032526A3 (cs) 2001-02-27 2003-12-17 Maxygen Aps Nové molekuly podobné interferonu beta
US20040117868A1 (en) * 2002-01-29 2004-06-17 Jun Imamura Protein participating in restoration from cytoplasmic male sterility to fertility and gene encoding the same
PT1382612E (pt) 2001-04-25 2011-09-19 Agronomique Inst Nat Rech Proteína que participa na reversão da esterilidade masculina citoplasmática para fertilidade e gene que codifica a referida proteína
US6995236B2 (en) 2001-05-08 2006-02-07 Riken Sphingomyelin detecting probe
WO2003027296A1 (en) * 2001-09-20 2003-04-03 Plantech Research Institute GENES PARTICIPATING IN THE SYNTHESIS OF FATTY ACID HAVING trans-11-, cis-13- CONDJUGATED DOUBLE BOND AND UTILIZATION THEREOF
JP2003144141A (ja) * 2001-11-14 2003-05-20 Gencom Co ＲＮＡｉ効果が増強したＥＳ細胞
DE60328595D1 (de) 2002-05-08 2009-09-10 Kyowa Hakko Bio Co Ltd Verfahren zur herstellung von cytidin 5'-diphosphatcholin
JP4171256B2 (ja) * 2002-07-18 2008-10-22 三菱化学株式会社 パピローマウイルスベクターを用いたＲＮＡｉ表現型を有する非ヒト哺乳動物の作製方法
CA2497338A1 (en) 2002-08-30 2004-03-18 Japan Science And Technology Corporation Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same
JP2004180641A (ja) * 2002-12-06 2004-07-02 Gencom Co ＲＮＡｉ表現型を有する非ヒト哺乳動物の作製方法
CN100400660C (zh) 2002-12-26 2008-07-09 协和发酵工业株式会社 生产二肽的方法
WO2004078965A1 (ja) * 2003-03-05 2004-09-16 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute 大腸菌における異種蛋白質の製造方法
MXPA06008496A (es) 2004-02-02 2007-01-30 Ambrx Inc Polipeptidos de interferon humano modificados y sus usos.
GT200600210A (es) * 2005-05-19 2007-01-03 Schering Ag Terapia genica de interferon-beta usdando un sistema mejorado de expresion regulada
CN101238214A (zh) * 2005-05-19 2008-08-06 拜尔先灵制药股份公司 使用改进的调节表达系统治疗疾病
US20070179113A1 (en) * 2005-05-19 2007-08-02 Schering Aktiengesellachaft GM-CSF gene therapy for Crohn's disease using an improved regulated expression system
US20080076729A1 (en) * 2005-05-19 2008-03-27 Schering Aktiengesellachaft Interferon-beta gene therapy using an improved, regulated expression system
EP1930344A4 (en) 2005-09-29 2008-12-31 Shionogi & Co POLYPEPTIDE HAVING ANTI-ANGIOGENIC ACTIVITY
JP5631534B2 (ja) 2005-11-29 2014-11-26 協和発酵バイオ株式会社 新規蛋白質および該蛋白質をコードするｄｎａ
US8093362B2 (en) 2005-12-06 2012-01-10 Kyowa Hakko Kirin Co., Ltd. Anti-PERP recombinant antibody
EP1978094B1 (en) 2005-12-27 2012-03-14 Kyowa Hakko Bio Co., Ltd. Method for production of l-glutamine
JP5586146B2 (ja) 2006-06-06 2014-09-10 協和発酵キリン株式会社 ヘパリン結合上皮細胞増殖因子様増殖因子に結合するモノクローナル抗体
US7939294B2 (en) 2006-09-25 2011-05-10 Kyowa Hakko Bio Co., Ltd. DNA encoding proteins having dipeptide-synthesizing activity and methods of using the same
WO2008090678A1 (ja) 2007-01-25 2008-07-31 Kyowa Hakko Kirin Co., Ltd. 新規ペプチド
WO2008114733A1 (ja) 2007-03-16 2008-09-25 Kyowa Hakko Kirin Co., Ltd. 抗Claudin-4抗体
JP5256192B2 (ja) 2007-03-29 2013-08-07 国立大学法人 岡山大学 バソヒビン含有治療剤
CN101715484B (zh) 2007-04-06 2014-02-19 协和发酵生化株式会社 二肽的制造方法
JP6018357B2 (ja) 2007-04-06 2016-11-02 協和発酵バイオ株式会社 グルタチオンおよびγ−グルタミルシステインの製造法
CN103965347B (zh) * 2007-05-02 2017-07-18 Ambrx公司 经修饰干扰素β多肽和其用途
US8383381B2 (en) 2007-09-27 2013-02-26 Shjonogi & Co., Ltd. Method for producing hydroxylated adaivjantane using cytochrome P450
JP5532401B2 (ja) 2007-12-05 2014-06-25 協和発酵キリン株式会社 ヘパリン結合上皮細胞増殖因子様増殖因子に結合するモノクローナル抗体
KR20110039220A (ko) 2008-06-30 2011-04-15 교와 핫꼬 기린 가부시키가이샤 항ｃｄ27 항체
EP2311946B1 (en) 2008-07-17 2016-04-06 Kyowa Hakko Kirin Co., Ltd. Anti-system asc amino acid transporter 2 (asct2) antibody
CN102232111B (zh) 2008-10-09 2014-12-03 协和梅迪克斯株式会社 果糖基肽氧化酶
PT2374883T (pt) 2008-12-26 2016-10-20 Kyowa Hakko Kirin Co Ltd Anticorpo anti-cd4
WO2010090330A1 (ja) 2009-02-09 2010-08-12 協和発酵バイオ株式会社 Ｌ－アミノ酸の製造法
EP2993188B1 (en) 2009-04-20 2020-08-19 Kyowa Kirin Co., Ltd. Anti cd40 antibody containing igg2 having three amino acid mutations introduced therein
KR101846590B1 (ko) 2010-06-11 2018-04-09 교와 핫꼬 기린 가부시키가이샤 항 ｔｉｍ-3 항체
JP5058332B2 (ja) 2010-07-14 2012-10-24 住友ゴム工業株式会社 イソプレンオリゴマー、ポリイソプレン、及びこれらの製造方法、ゴム組成物、並びに空気入りタイヤ
EP2722343A4 (en) 2011-06-20 2014-12-17 Kyowa Hakko Kirin Co Ltd ANTI-ERBB3 ANTIBODIES
CA2874117C (en) 2012-07-02 2021-10-12 Kyowa Hakko Kirin Co., Ltd. Pharmaceutical agent comprising anti-bmp9 antibody as active ingredient for treatment of anemia such as renal anemia and cancer anemia
KR20150094617A (ko) 2012-12-07 2015-08-19 교와 핫꼬 기린 가부시키가이샤 항 ｆｏｌｒ１ 항체
WO2015005258A1 (ja) 2013-07-09 2015-01-15 協和メデックス株式会社 糖化ヘキサペプチドオキシダーゼとその利用
WO2017150695A1 (ja) 2016-03-04 2017-09-08 国立大学法人新潟大学 変異型テトラプレニル－β－クルクメン環化酵素及びアンブレインの製造方法
EP3492591A4 (en) 2016-07-26 2020-04-01 Shizuoka Prefecture ANTI-B7-H4 ANTIBODIES
SG11201901093PA (en) 2016-08-10 2019-03-28 Spiber Inc Production method for insoluble recombinant protein aggregate
WO2018066558A1 (ja) 2016-10-03 2018-04-12 Ｓｐｉｂｅｒ株式会社 組換えタンパク質の精製方法
CN110494553B (zh) 2017-04-13 2023-07-04 协和发酵生化株式会社 茶氨酸的制造方法
US11091545B2 (en) 2017-07-13 2021-08-17 Kyowa Kirin Co., Ltd. Methods of using an antibody to stabilize and analyze a BRIL fusion protein
WO2019017401A1 (ja) 2017-07-18 2019-01-24 協和発酵キリン株式会社 抗ヒトｃｃｒ１モノクローナル抗体
BR112020001627A2 (pt) 2017-07-26 2020-07-21 Spiber Inc. fibroína modificada, ácido nucleico, vetor de expressão, hospedeiro, produto, fibra de fibroína artificialmente modificada, e, método para a produção de uma fibra de fibroína artificialmente modificada.
CN111094571B (zh) 2017-09-01 2023-10-27 国立大学法人新潟大学 龙涎香醇的有效制备方法
JP7311425B2 (ja) 2017-11-08 2023-07-19 協和キリン株式会社 ＣＤ４０とＥｐＣＡＭに結合するバイスペシフィック抗体
AU2018384239B2 (en) 2017-12-12 2025-04-24 Kyowa Kirin Co., Ltd. Anti-BMP10 antibody, and therapeutic agent for hypertension and hypertensive diseases comprising said antibody as active ingredient
EP3816291B1 (en) 2018-06-26 2025-12-31 Kyowa Kirin Co., Ltd. ANTIBODIES BINDING TO PROTEOGLYCAN-5 WITH CHONDROITINE SULFATE
CA3105000A1 (en) 2018-06-26 2020-01-02 Kyowa Kirin Co., Ltd. Antibody binding to cell adhesion molecule 3
JP7540943B2 (ja) 2018-08-10 2024-08-27 協和発酵バイオ株式会社 多価不飽和脂肪酸を生産する微生物及び多価不飽和脂肪酸の製造法
US11613728B2 (en) 2018-08-10 2023-03-28 Kyowa Hakko Bio Co., Ltd. Microorganism producing eicosapentaenoic acid and method for producing eicosapentaenoic acid
EP3903816A4 (en) 2018-12-28 2022-09-07 Kyowa Kirin Co., Ltd. BISPECIFIC ANTIBODY BINDING TO TFR
JPWO2020145363A1 (ja) 2019-01-09 2021-11-25 Ｓｐｉｂｅｒ株式会社 改変フィブロイン
JP6968468B2 (ja) 2019-01-31 2021-11-17 Ｓｐｉｂｅｒ株式会社 組換えタンパク質の製造方法
KR20220008820A (ko) 2019-05-15 2022-01-21 쿄와 기린 가부시키가이샤 Cd40과 fap에 결합하는 이중 특이적 항체
EP3971293A4 (en) 2019-05-15 2023-02-08 Kyowa Kirin Co., Ltd. BISPECIFIC ANTIBODIES CAPABLE OF BINDING TO CD40 AND GPC3
US20240279696A1 (en) 2019-12-16 2024-08-22 Kyowa Hakko Bio Co., Ltd. Microorganism having modified lactose permease, and method for producing lactose-containing oligosaccharide
TWI888487B (zh) 2020-02-14 2025-07-01 日商協和麒麟股份有限公司 與ｃｄ３結合之雙特異性抗體
KR20220155328A (ko) 2020-03-16 2022-11-22 스파이버 가부시키가이샤 합성 고분자 및 그의 제조 방법, 성형 재료, 그리고, 성형체
CN115667517A (zh) 2020-06-25 2023-01-31 协和发酵生化株式会社 二肽的制造方法
JPWO2022168991A1 (enExample) 2021-02-08 2022-08-11
JPWO2023027177A1 (enExample) 2021-08-26 2023-03-02
CA3231307A1 (en) 2021-09-10 2023-03-16 Kyowa Hakko Bio Co., Ltd. Recombinant microorganism used for producing cdp-choline, and method for producing cdp-choline using said recombinant microorganism
MX2024009817A (es) 2022-02-09 2024-08-19 Nat Inst Biomedical Innovation Health & Nutrition Anticuerpo o fragmento del mismo que se une a fcrl1.
CN118984876A (zh) 2022-04-28 2024-11-19 协和发酵生化株式会社 具有nampt活性的蛋白质和nmn的制造方法
JPWO2023238843A1 (enExample) 2022-06-06 2023-12-14
EP4541902A1 (en) 2022-06-30 2025-04-23 Kirin Holdings Kabushiki Kaisha Method for producing 4-(aminomethyl)cyclohexane-1-carboxylic acid
EP4570910A1 (en) 2022-08-10 2025-06-18 Kyowa Kirin Co., Ltd. Anti-fgf23 antibody, and fragment of said antibody
JPWO2024122636A1 (enExample) 2022-12-09 2024-06-13
WO2025005153A1 (ja) 2023-06-29 2025-01-02 キリンホールディングス株式会社 ４－（アミノメチル）シクロヘキサン－１－カルボン酸の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE52036B1 (en) * 1979-05-24 1987-05-27 Univ California Non-passageable viruses
GB2052516B (en) * 1979-06-01 1983-06-29 Searle & Co Plasmid vectors production and use thereof
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
CA1341604C (en) * 1980-04-03 2010-05-04 Biogen N.V. Dna sequences, recombinant dna molecules and processes for producing human fibroblast interferon-like polypeptides
IT1139487B (it) * 1980-09-25 1986-09-24 Genentech Inc Produzione microbica di interferone di fibroblasti umani

Also Published As

Publication number Publication date
DE3277307D1 (en) 1987-10-22
EP0083069A3 (en) 1984-03-28
JPS58110600A (ja) 1983-07-01
EP0083069B1 (en) 1987-09-16
US4686191A (en) 1987-08-11
EP0083069A2 (en) 1983-07-06
CA1211059A (en) 1986-09-09

Similar Documents

Publication Publication Date Title
JPH0134599B2 (enExample)
JP2686090B2 (ja) 新規融合蛋白質およびその精製方法
US5122457A (en) Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
EP0198015B2 (en) Production of streptavidin-like polypeptides
JP2887248B2 (ja) ポリペプチドの製造方法
EP0160571A2 (en) Cloning vehicle
CA1198068A (en) Bacterial polypeptide expression employing tryptophan promoter-operator
IL173399A0 (en) Circular dna molecule with conditional origin of replication which contains nucleic acid of interest and selection marker but does not encode its replication initiation protein, method for preparing the same and use thereof in gene therapy
JPH0669375B2 (ja) 特異的切断リンカ−
JPH0248235B2 (enExample)
JPH0665305B2 (ja) 組換えｄｎａ含有宿主細胞の安定化法
KR890014738A (ko) 단백질 합성의 cks 방법
JP3837154B2 (ja) 遺伝子発現用プロモーター
US4716112A (en) Vectors for increased expression of cloned genes
US4795706A (en) Novel expression control sequences
Rosenberg et al. T7 RNA polymerase can direct expression of influenza virus cap-binding protein (PB2) in Escherichia coli
GB2024229A (en) Process for producing proteins by the expression of the corresponding ADN in bacteria, modified DNA and vectors applicable in such processes
Trojanowska et al. The bacteriophage T4 regA gene: primary sequence of a translational repressor
JPH0829099B2 (ja) 組換ｄｎａ、発現ベクター及びプラスミド、これらの製法及び誘発可能で抑制可能な外来遺伝子の発現法
Nishi et al. Construction and application of a novel plasmid" ATG vector" for direct expression of foreign genes in Escherichia coli
EP0314184B1 (en) Expression plasmids
KR830007827A (ko) 신규 재조합 플라스미드의 제조방법
JPH02100685A (ja) ポリペプチド分泌発現ベクター，該ベクターで形質転換した微生物及び該微生物によるポリペプチドの製造
DE3882465D1 (de) Expression von mutarotase.
Fraser The future of recombinant DNA technology in medicine