CA1211059A - RECOMBINANT PLASMID CONTAINING HUMAN INTERFERON-.beta. GENE - Google Patents

RECOMBINANT PLASMID CONTAINING HUMAN INTERFERON-.beta. GENE

Info

Publication number
CA1211059A
CA1211059A CA000418366A CA418366A CA1211059A CA 1211059 A CA1211059 A CA 1211059A CA 000418366 A CA000418366 A CA 000418366A CA 418366 A CA418366 A CA 418366A CA 1211059 A CA1211059 A CA 1211059A
Authority
CA
Canada
Prior art keywords
dna
beta
plasm
human interferon
promoter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
CA000418366A
Other languages
English (en)
French (fr)
Inventor
Seiga Itoh
Tatsunari Nishi
Tamio Mizukami
Tadashi Matsumoto
Tetsuo Oka
Tadatsugu Taniguchi
Haruo Sugano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japanese Foundation for Cancer Research
KH Neochem Co Ltd
Original Assignee
Japanese Foundation for Cancer Research
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japanese Foundation for Cancer Research, Kyowa Hakko Kogyo Co Ltd filed Critical Japanese Foundation for Cancer Research
Application granted granted Critical
Publication of CA1211059A publication Critical patent/CA1211059A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/14—Lymphokine; related peptides
    • Y10S930/142—Interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
CA000418366A 1981-12-25 1982-12-22 RECOMBINANT PLASMID CONTAINING HUMAN INTERFERON-.beta. GENE Expired CA1211059A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP56213193A JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
JP213193/81 1981-12-25

Publications (1)

Publication Number Publication Date
CA1211059A true CA1211059A (en) 1986-09-09

Family

ID=16635072

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000418366A Expired CA1211059A (en) 1981-12-25 1982-12-22 RECOMBINANT PLASMID CONTAINING HUMAN INTERFERON-.beta. GENE

Country Status (5)

Country Link
US (1) US4686191A (enExample)
EP (1) EP0083069B1 (enExample)
JP (1) JPS58110600A (enExample)
CA (1) CA1211059A (enExample)
DE (1) DE3277307D1 (enExample)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58141796A (ja) * 1982-02-18 1983-08-23 Kyowa Hakko Kogyo Co Ltd ペプチドの製造法
EP0121569B1 (en) * 1982-10-08 1990-08-22 Kyowa Hakko Kogyo Co., Ltd. Novel vector
JPH0740925B2 (ja) * 1983-03-14 1995-05-10 協和醗酵工業株式会社 新規ヒトインタ−フエロン−γポリペプチド
JPS59203495A (ja) * 1983-04-28 1984-11-17 Kyowa Hakko Kogyo Co Ltd 外来遺伝子の新規な発現方法
WO1985001066A1 (fr) * 1983-08-22 1985-03-14 Kyowa Hakko Kogyo Co., Ltd. Procede de production de peptides
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
DE3585176D1 (de) * 1984-06-22 1992-02-27 Hitachi Ltd Verfahren zum kontrollieren der zuechtung von rekombinanten.
FR2579224B1 (fr) * 1985-03-25 1987-05-22 Genetica Procede de preparation microbiologique de la serum-albumine humaine
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
NO176799C (no) 1986-12-23 1995-05-31 Kyowa Hakko Kogyo Kk DNA som koder for et polypeptid, rekombinant plasmid som koder for og kan uttrykke et polypeptid og fremgangsmåte for fremstilling av dette polypeptid
DE4128319A1 (de) * 1991-08-27 1993-03-04 Bioferon Biochem Substanz Neues rekombinantes human-ifn-beta, verfahren zu dessen herstellung und dieses enthaltende pharmazeutische zubereitungen
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
CA2736981A1 (en) 1998-04-14 1999-10-21 Kyowa Hakko Bio Co., Ltd. Process for producing isoprenoid compounds by microorganisms
AU3956399A (en) 1998-06-02 1999-12-20 Kyowa Hakko Kogyo Co. Ltd. Iga nephropathy-associated dna
US7144574B2 (en) * 1999-08-27 2006-12-05 Maxygen Aps Interferon β variants and conjugates
US7431921B2 (en) 1999-08-27 2008-10-07 Maxygen Aps Interferon beta-like molecules
US6531122B1 (en) 1999-08-27 2003-03-11 Maxygen Aps Interferon-β variants and conjugates
EP1295939A4 (en) 2000-06-15 2005-02-02 Kyowa Hakko Kogyo Kk INSULIN SIMILAR GROWTH FACTOR BINDING PROTEIN
FR2810674A1 (fr) * 2000-06-22 2001-12-28 Pf Medicament Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
US20020169290A1 (en) * 2000-11-02 2002-11-14 Claus Bornaes New multimeric interferon beta polypeptides
EP1371727A4 (en) * 2001-02-22 2004-07-28 Gencom Corp RECOMBINANT GENE CONTAINING A REVERSE REPETITION SEQUENCE AND USE THEREOF
PL367154A1 (en) 2001-02-27 2005-02-21 Maxygen Aps New interferon beta-like molecules
AU2002251533B2 (en) 2001-04-25 2006-09-28 Institut National De La Recherche Agronomique Protein involved in restoration of cytoplasmic male sterility to fertility and gene encoding the protein and gene
US20040117868A1 (en) * 2002-01-29 2004-06-17 Jun Imamura Protein participating in restoration from cytoplasmic male sterility to fertility and gene encoding the same
US6995236B2 (en) 2001-05-08 2006-02-07 Riken Sphingomyelin detecting probe
WO2003027296A1 (en) * 2001-09-20 2003-04-03 Plantech Research Institute GENES PARTICIPATING IN THE SYNTHESIS OF FATTY ACID HAVING trans-11-, cis-13- CONDJUGATED DOUBLE BOND AND UTILIZATION THEREOF
JP2003144141A (ja) * 2001-11-14 2003-05-20 Gencom Co ＲＮＡｉ効果が増強したＥＳ細胞
ATE437956T1 (de) 2002-05-08 2009-08-15 Kyowa Hakko Bio Co Ltd Verfahren zur herstellung von cytidin 5'- diphosphatcholin
JP4171256B2 (ja) * 2002-07-18 2008-10-22 三菱化学株式会社 パピローマウイルスベクターを用いたＲＮＡｉ表現型を有する非ヒト哺乳動物の作製方法
US20060248617A1 (en) 2002-08-30 2006-11-02 Japan Science And Technology Corporation Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same
JP2004180641A (ja) * 2002-12-06 2004-07-02 Gencom Co ＲＮＡｉ表現型を有する非ヒト哺乳動物の作製方法
JP4447467B2 (ja) 2002-12-26 2010-04-07 協和発酵バイオ株式会社 ジペプチドの製造法
JP4463198B2 (ja) * 2003-03-05 2010-05-12 財団法人化学及血清療法研究所 大腸菌における異種蛋白質の製造方法
CN103755800B (zh) 2004-02-02 2016-02-24 Ambrx公司 经修饰的人类生长激素多肽与其用途
CN101238214A (zh) * 2005-05-19 2008-08-06 拜尔先灵制药股份公司 使用改进的调节表达系统治疗疾病
GT200600210A (es) * 2005-05-19 2007-01-03 Schering Ag Terapia genica de interferon-beta usdando un sistema mejorado de expresion regulada
US20070179113A1 (en) * 2005-05-19 2007-08-02 Schering Aktiengesellachaft GM-CSF gene therapy for Crohn's disease using an improved regulated expression system
US20080076729A1 (en) * 2005-05-19 2008-03-27 Schering Aktiengesellachaft Interferon-beta gene therapy using an improved, regulated expression system
JPWO2007037245A1 (ja) 2005-09-29 2009-04-09 国立大学法人東北大学 血管新生抑制作用を有するポリペプチド
CN101351551A (zh) 2005-11-29 2009-01-21 协和发酵工业株式会社 新型蛋白质和编码该蛋白质的dna
US8093362B2 (en) 2005-12-06 2012-01-10 Kyowa Hakko Kirin Co., Ltd. Anti-PERP recombinant antibody
ES2382207T3 (es) 2005-12-27 2012-06-06 Kyowa Hakko Bio Co., Ltd. Procedimiento para la producción de L-glutamina
KR20090029227A (ko) 2006-06-06 2009-03-20 교와 핫꼬 기린 가부시키가이샤 헤파린 결합 상피세포 증식 인자 유사 증식 인자에 결합하는 모노클로날 항체
EP2067857B1 (en) 2006-09-25 2012-04-11 Kyowa Hakko Bio Co., Ltd Method for production of dipeptide
WO2008090678A1 (ja) 2007-01-25 2008-07-31 Kyowa Hakko Kirin Co., Ltd. 新規ペプチド
JPWO2008114733A1 (ja) 2007-03-16 2010-07-01 協和発酵キリン株式会社 抗Ｃｌａｕｄｉｎ−４抗体
US20100113354A1 (en) 2007-03-29 2010-05-06 Yasufumi Sato Therapeutic agent comprising vasohibin
CN101715490B (zh) 2007-04-06 2014-02-05 协和发酵生化株式会社 谷胱甘肽及γ-谷氨酰半胱氨酸的制造方法
WO2008126783A1 (ja) 2007-04-06 2008-10-23 Kyowa Hakko Bio Co., Ltd. ジペプチドの製造法
KR20100019467A (ko) * 2007-05-02 2010-02-18 암브룩스, 인코포레이티드 변형된 인터페론 베타 폴리펩티드 및 그의 용도
US8383381B2 (en) 2007-09-27 2013-02-26 Shjonogi & Co., Ltd. Method for producing hydroxylated adaivjantane using cytochrome P450
AU2008332296A1 (en) 2007-12-05 2009-06-11 Kyowa Hakko Kirin Co., Ltd. Monoclonal antibody capable of binding to heparin-binding epidermal growth factor-like growth factor
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US8268592B2 (en) 2008-07-17 2012-09-18 Kyowa Hakko Kirin Co., Ltd Anti-system ASC amino acid transporter 2 (ASCT2) antibody
WO2010041715A1 (ja) 2008-10-09 2010-04-15 協和メデックス株式会社 新規フルクトシルペプチドオキシダーゼ
HUE029946T2 (en) 2008-12-26 2017-04-28 Kyowa Hakko Kirin Co Ltd Anti-CD4 antibody
EP2395096B1 (en) 2009-02-09 2014-04-09 Kyowa Hakko Bio Co., Ltd. Process for producing L-amino acids
EP2993188B1 (en) 2009-04-20 2020-08-19 Kyowa Kirin Co., Ltd. Anti cd40 antibody containing igg2 having three amino acid mutations introduced therein
WO2011155607A1 (ja) 2010-06-11 2011-12-15 協和発酵キリン株式会社 抗ｔｉｍ－３抗体
JP5058332B2 (ja) 2010-07-14 2012-10-24 住友ゴム工業株式会社 イソプレンオリゴマー、ポリイソプレン、及びこれらの製造方法、ゴム組成物、並びに空気入りタイヤ
KR20140033152A (ko) 2011-06-20 2014-03-17 교와 핫꼬 기린 가부시키가이샤 항erbB3 항체
CN104411720B (zh) 2012-07-02 2018-05-11 协和发酵麒麟株式会社 以抗bmp9抗体作为有效成分的、对肾性贫血、癌性贫血等贫血的治疗剂
CA2893378A1 (en) 2012-12-07 2014-06-12 Kyowa Hakko Kirin Co., Ltd. Anti-folr1 antibody
JP6328115B2 (ja) 2013-07-09 2018-05-23 協和メデックス株式会社 糖化ヘキサペプチドオキシダーゼとその利用
MX395506B (es) 2016-03-04 2025-03-25 Univ Niigata CICLASA DE TETRAPRENIL-ß-CURCUMENO MUTADA Y MÉTODO PARA PRODUCIR AMBREÍNA.
WO2018021301A1 (ja) 2016-07-26 2018-02-01 静岡県 抗ｂ７－ｈ４抗体
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JP7035024B2 (ja) 2017-04-13 2022-03-14 協和発酵バイオ株式会社 テアニンの製造方法
CN110914430B (zh) 2017-07-13 2023-11-21 协和麒麟株式会社 抗bril抗体以及使用了该抗体的bril融合蛋白质的稳定化方法
CA3070342A1 (en) 2017-07-18 2019-01-24 Kyowa Kirin Co., Ltd. Anti-human ccr1 monoclonal antibody
BR112020001627A2 (pt) 2017-07-26 2020-07-21 Spiber Inc. fibroína modificada, ácido nucleico, vetor de expressão, hospedeiro, produto, fibra de fibroína artificialmente modificada, e, método para a produção de uma fibra de fibroína artificialmente modificada.
WO2019045058A1 (ja) 2017-09-01 2019-03-07 国立大学法人新潟大学 アンブレインの効率的製造方法
KR102782136B1 (ko) 2017-11-08 2025-03-18 쿄와 기린 가부시키가이샤 CD40과 EpCAM에 결합하는 이중특이적 항체
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CN112424358B (zh) 2018-06-26 2025-07-01 协和麒麟株式会社 与细胞粘附分子3结合的抗体
JP7397444B2 (ja) 2018-06-26 2023-12-13 協和キリン株式会社 コンドロイチン硫酸プロテオグリカン－５に結合する抗体
US12241101B2 (en) 2018-08-10 2025-03-04 Kyowa Hakko Bio Co., Ltd. Microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid
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US12319718B2 (en) 2019-01-09 2025-06-03 Spiber Inc. Modified fibroin
US20220033870A1 (en) 2019-01-31 2022-02-03 Spiber Inc. Method for producing recombinant protein
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US12466891B2 (en) 2019-05-15 2025-11-11 Kyowa Kirin Co., Ltd. Bispecific antibody binding to CD40 and GPC3
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EP4122948A4 (en) 2020-03-16 2024-05-01 Spiber Inc. Synthetic polymer and method for producing same, molding material, and molded body
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Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE52036B1 (en) * 1979-05-24 1987-05-27 Univ California Non-passageable viruses
GB2052516B (en) * 1979-06-01 1983-06-29 Searle & Co Plasmid vectors production and use thereof
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
DE3177213D1 (de) * 1980-04-03 1990-10-18 Biogen Inc Dns-sequenzen, rekombinante dns-molekuele und verfahren zur herstellung von dem menschlichen fibroblast-interferon.
FR2490675B1 (fr) * 1980-09-25 1985-11-15 Genentech Inc Production microbienne d'interferon de fibroplaste humain

Also Published As

Publication number Publication date
US4686191A (en) 1987-08-11
DE3277307D1 (en) 1987-10-22
EP0083069B1 (en) 1987-09-16
JPS58110600A (ja) 1983-07-01
JPH0134599B2 (enExample) 1989-07-20
EP0083069A2 (en) 1983-07-06
EP0083069A3 (en) 1984-03-28

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