ATE486936T1 - Verfahren zur herstellung eines heterologen proteins in e. coli - Google Patents

Verfahren zur herstellung eines heterologen proteins in e. coli

Info

Publication number
ATE486936T1
ATE486936T1 AT04713206T AT04713206T ATE486936T1 AT E486936 T1 ATE486936 T1 AT E486936T1 AT 04713206 T AT04713206 T AT 04713206T AT 04713206 T AT04713206 T AT 04713206T AT E486936 T1 ATE486936 T1 AT E486936T1
Authority
AT
Austria
Prior art keywords
heterologous protein
producing
protein
inducer
recombinant
Prior art date
Application number
AT04713206T
Other languages
English (en)
Inventor
Satoshi Koyanagi
Keishin Sugawara
Yoshinobu Miyatsu
Toshio Murakami
Toshihiro Maeda
Hiroshi Mizokami
Original Assignee
Chemo Sero Therapeut Res Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeut Res Inst filed Critical Chemo Sero Therapeut Res Inst
Application granted granted Critical
Publication of ATE486936T1 publication Critical patent/ATE486936T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43531—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
AT04713206T 2003-03-05 2004-02-20 Verfahren zur herstellung eines heterologen proteins in e. coli ATE486936T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003058992 2003-03-05
PCT/JP2004/002017 WO2004078965A1 (ja) 2003-03-05 2004-02-20 大腸菌における異種蛋白質の製造方法

Publications (1)

Publication Number Publication Date
ATE486936T1 true ATE486936T1 (de) 2010-11-15

Family

ID=32958810

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04713206T ATE486936T1 (de) 2003-03-05 2004-02-20 Verfahren zur herstellung eines heterologen proteins in e. coli

Country Status (6)

Country Link
US (1) US20070065906A1 (de)
EP (1) EP1602720B1 (de)
JP (1) JP4463198B2 (de)
AT (1) ATE486936T1 (de)
DE (1) DE602004029868D1 (de)
WO (1) WO2004078965A1 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2054726B1 (de) * 2006-08-14 2010-10-27 BioControl Systems, Inc. Verfahren zur erkennung von listerien

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58110600A (ja) * 1981-12-25 1983-07-01 Kyowa Hakko Kogyo Co Ltd ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
US4806471A (en) * 1982-09-16 1989-02-21 A/S Alfred Benzon Plasmids with conditional uncontrolled replication behavior
GB8308236D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Dna vectors
US4767708A (en) * 1984-08-07 1988-08-30 Carnegie Mellon University Enzyme amplification and purification
US5244806A (en) * 1985-08-26 1993-09-14 Eli Lilly And Company DNA encoding novel tissue plasminogen activator derivatives having kringles 1 and 2 deleted, vectors and host cells
US5210029A (en) * 1985-10-03 1993-05-11 Takeda Chemical Industries, Ltd. Method of producing interleukin-2
GB8611832D0 (en) * 1986-05-15 1986-06-25 Holland I B Polypeptide
ES2033747T3 (es) * 1986-07-22 1993-04-01 Ciba-Geigy Ag Preparacion de polipeptidos relacionados con factores de ligacion.
GB8701848D0 (en) * 1987-01-28 1987-03-04 Celltech Ltd Polypeptides
JP2676511B2 (ja) * 1987-03-23 1997-11-17 株式会社日立製作所 酢酸を指標とした培養方法及びその装置
DE3723992A1 (de) * 1987-07-20 1989-02-02 Boehringer Mannheim Gmbh Verfahren zur herstellung von proteinen in loeslicher form
FI88309C (fi) * 1989-04-20 1993-04-26 Bio Orbit Oy Ny biotest
US5714465A (en) * 1989-05-19 1998-02-03 Amgen Inc. Method of inhibiting tumor cell dissemination with a metalloproteinase inhibitor
US5641653A (en) * 1989-10-31 1997-06-24 The Texas A&M University System DNA encoding Actinobacillus pleuropneumoniae hemolysin
WO1992004445A1 (en) * 1990-09-11 1992-03-19 The Western Australian Research Institute For Child Health Ltd. Cloning and sequencing of allergens of dermatophagoides (house dust mite)
GB9004390D0 (en) * 1990-02-27 1990-04-25 Ici Plc Process
JP3139777B2 (ja) * 1990-08-27 2001-03-05 フマキラー株式会社 組換えダニアレルゲン
JPH0771482B2 (ja) * 1991-09-03 1995-08-02 工業技術院長 組換え菌体の培養方法
JP2657861B2 (ja) * 1991-09-17 1997-09-30 アサヒビール株式会社 ダニ主要アレルゲンの製造方法
CA2078721A1 (en) * 1991-09-24 1993-03-25 Hiroshi Yonemura Process for preparing human coagulation factor viii protein complex
US7288256B1 (en) * 1991-10-16 2007-10-30 Merck Patent Gmbh T cell epitopes of the major allergens from dermatophagoides (house dust mite)
GB9215541D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
GB9215540D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
JPH06141869A (ja) * 1992-10-30 1994-05-24 Daikin Ind Ltd 耐熱性供与遺伝子
SE9203753D0 (sv) * 1992-12-11 1992-12-11 Kabi Pharmacia Ab Expression system for producing apolipoprotein ai-m
US6180771B1 (en) * 1993-12-08 2001-01-30 Immulogic Pharmaceutical Corp. Nucleic acids encoding a house dust mite allergen, Der p III, and uses therefor
US5968526A (en) * 1994-04-14 1999-10-19 Immulogic Pharamaceutical Corporation T cell epitopes of the major allergens from Dermatophagoides (house dust mite)
JP3723240B2 (ja) * 1994-06-01 2005-12-07 第一サントリーファーマ株式会社 遺伝子発現の制御方法
US6287574B1 (en) * 1995-03-17 2001-09-11 Biochem Pharma Inc. Proteinase K resistant surface protein of neisseria meningitidis
WO1996030539A1 (fr) * 1995-03-28 1996-10-03 Asahi Breweries, Ltd. Allergene d'acarien obtenu grace au genie genetique et son procede de fabrication
JPH0940583A (ja) * 1995-07-31 1997-02-10 Asahi Breweries Ltd 組換えＤｅｒ ｆ ＩＩＩアレルゲン及びその製造法
GB2304718B (en) * 1995-09-05 2000-01-19 Degussa The production of tryptophan by the bacterium escherichia coli
KR19990076627A (ko) * 1995-12-22 1999-10-15 미리암 디. 메코너헤이 재조합 배큘로바이러스의 제조
JP3805466B2 (ja) * 1997-02-07 2006-08-02 アサヒビール株式会社 組換えダニアレルゲンＤｅｒ ｆ １およびその製造方法
US5994511A (en) * 1997-07-02 1999-11-30 Genentech, Inc. Anti-IgE antibodies and methods of improving polypeptides
US6172213B1 (en) * 1997-07-02 2001-01-09 Genentech, Inc. Anti-IgE antibodies and method of improving polypeptides
CA2251263A1 (en) * 1997-11-14 1999-05-14 Hajime Karasuyama Transgenic animal allergy models and methods for their use
SE515819C2 (sv) * 1999-02-25 2001-10-15 Statens Veterinaermedicinska A Protein från kvalstret Sarcoptes scabiei
EP1171577A2 (de) * 1999-04-09 2002-01-16 PHARMACIA & UPJOHN COMPANY Antibakterielle vakzinzusammensetzungen
US20020090615A1 (en) * 2000-01-31 2002-07-11 Rosen Craig A. Nucleic acids, proteins, and antibodies
JP3532510B2 (ja) * 2000-07-21 2004-05-31 独立行政法人農業・生物系特定産業技術研究機構 組換え豚丹毒菌防御抗原の回収精製法
US6673580B2 (en) * 2000-10-27 2004-01-06 Genentech, Inc. Identification and modification of immunodominant epitopes in polypeptides
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
ATE448793T1 (de) * 2001-03-02 2009-12-15 Univ Rockefeller Rekombinante hybrid-allergenkonstrukte mit verringerter allergenität unter beibehaltung der immunogenität des natürlichen allergens
WO2002088303A2 (en) * 2001-04-03 2002-11-07 Bristol-Myers Squibb Company Polynucleotide encoding a novel cysteine protease of the calpain superfamily, can-12, and variants thereof
AU2002258780A1 (en) * 2001-04-11 2002-10-28 Bristol-Myers Squibb Company Polynucleotides encoding two novel human g-protein coupled receptors, hgprbmy28 and hgprbmy29, and splice variants thereof
JP2003284570A (ja) * 2001-04-25 2003-10-07 Chemo Sero Therapeut Res Inst フォンビルブラント因子（ｖＷＦ）切断酵素
US20070196353A1 (en) * 2001-05-25 2007-08-23 Hugh Payton Method for reducing allergenicity in indoor spaces
BR0311995A (pt) * 2002-06-20 2005-04-05 Cytos Biotechnology Ag Partìculas semelhantes a vìrus empacotadas para o uso como adjuvantes: método de preparação e uso
EP1382613A1 (de) * 2002-07-16 2004-01-21 Sumitomo Chemical Company, Limited Neues G Protein, kodierende Nukleotidsequenzen und Verwendung des Proteins
CA2559379A1 (en) * 2004-01-20 2005-07-28 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Process for producing human serum albumin by heating in presence of divalent cation
US7604798B2 (en) * 2004-07-15 2009-10-20 Northwestern University Methods and compositions for importing nucleic acids into cell nuclei
US8329162B2 (en) * 2006-07-21 2012-12-11 California Institute Of Technology Targeted gene delivery for dendritic cell vaccination

Also Published As

Publication number Publication date
WO2004078965A1 (ja) 2004-09-16
JP4463198B2 (ja) 2010-05-12
EP1602720A4 (de) 2008-06-18
EP1602720B1 (de) 2010-11-03
DE602004029868D1 (de) 2010-12-16
JPWO2004078965A1 (ja) 2006-06-08
US20070065906A1 (en) 2007-03-22
EP1602720A1 (de) 2005-12-07

Similar Documents

Publication Publication Date Title
ATE368730T1 (de) Verfahren zur herstelllung rekombinanter proteine in eukaryontischen zellen
ATE110773T1 (de) Verfahren zur züchtung rekombinanter zellen.
ATE521701T1 (de) Verbesserte konstrukte zur expression lysosomaler polypeptide
DE59608743D1 (de) Verfahren zur herstellung von rekombinanten proteinen in e. coli mittels hochzelldichte-fermentation
DE602006018587D1 (de) Verfahren zur verbesserten isolierung rekombinant produzierter proteine
ATE119198T1 (de) Verfahren zur herstellung von proteinen mittels rekombinanter dna.
ATE299942T1 (de) Verfahren zur herstellung von natürlich gefalteten proteinen durch co-sekretion von molekularen chaperonen
DE69839553D1 (de) Verfahren zur herstellung von proteinen
DK1616878T3 (da) Udskilte og membranspændende polypeptider samt nukleinsyrer kodende for disse
DK1246917T3 (da) Humane Stra6-polypeptider
ATE486944T1 (de) Translationselongationsfaktor-promotor aus pichia pastoris und verfahren zur herstellung von rekombinantem protein unter verwendung davon
PT1025245E (pt) Criacao rapida de linhas celulares estaveis de mamifero produzindo elevados iiveis de proteinas recombinantes
ATE454444T1 (de) Verfahren zur herstellung rekombinanter proteine
ATE493497T1 (de) Verfahren zur herstellung von oligopeptiden
DE60026733D1 (de) Histone-deacetylase-8 proteine, nukleinsäuren und methoden zur verwendung
ATE486936T1 (de) Verfahren zur herstellung eines heterologen proteins in e. coli
WO2004024898A3 (en) Nematode fatty acid desaturase-like sequences
DE69838644D1 (de) Verfahren zur herstellung eines rekombinanten rinderadenovirus-vektors
ATE142263T1 (de) Verfahren zur herstellung von fremdproteinen in streptomyceten
ATE321854T1 (de) Hypophysentumor verursachende gene und verwandte produkte
ATE312939T1 (de) Verfahren zur rekombinanten herstellung von polypeptiden
EE03074B1 (et) Rekombinantsed eukarüootsed rakud, nende saamiseks vajalikud DNA järjestused, meetod eukarüootsete rakkude konstrueerimiseks ja sekreteeritavate valkude ning biomassi tootmismeetod
ATE444370T1 (de) Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments
BRPI0516805A (pt) processo para a produção de uma proteìna heteróloga utilizando células hospedeiras de uma espécie de levedura
ATE445017T1 (de) Methode zur herstellung eines polypeptides, einer rna oder einer anderen verbindung in tumorgewebe

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties