JP6693743B2 - 細胞培養上清の濾過 - Google Patents
細胞培養上清の濾過 Download PDFInfo
- Publication number
- JP6693743B2 JP6693743B2 JP2015522110A JP2015522110A JP6693743B2 JP 6693743 B2 JP6693743 B2 JP 6693743B2 JP 2015522110 A JP2015522110 A JP 2015522110A JP 2015522110 A JP2015522110 A JP 2015522110A JP 6693743 B2 JP6693743 B2 JP 6693743B2
- Authority
- JP
- Japan
- Prior art keywords
- filter
- cells
- bar
- pore size
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12177277.6A EP2687592A1 (de) | 2012-07-20 | 2012-07-20 | Filtration von Zellkulturüberständen |
| EP12177277.6 | 2012-07-20 | ||
| PCT/EP2013/065264 WO2014013045A1 (de) | 2012-07-20 | 2013-07-19 | Filtration von zellkulturüberständen |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018112570A Division JP2018157827A (ja) | 2012-07-20 | 2018-06-13 | 細胞培養上清の濾過 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015522287A JP2015522287A (ja) | 2015-08-06 |
| JP6693743B2 true JP6693743B2 (ja) | 2020-05-13 |
Family
ID=48803559
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015522110A Active JP6693743B2 (ja) | 2012-07-20 | 2013-07-19 | 細胞培養上清の濾過 |
| JP2018112570A Pending JP2018157827A (ja) | 2012-07-20 | 2018-06-13 | 細胞培養上清の濾過 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018112570A Pending JP2018157827A (ja) | 2012-07-20 | 2018-06-13 | 細胞培養上清の濾過 |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US10081789B2 (enExample) |
| EP (2) | EP2687592A1 (enExample) |
| JP (2) | JP6693743B2 (enExample) |
| KR (1) | KR102087258B1 (enExample) |
| CN (1) | CN104769102B (enExample) |
| AU (1) | AU2013291990B2 (enExample) |
| BR (1) | BR112015001120B1 (enExample) |
| CA (1) | CA2882100C (enExample) |
| DK (1) | DK2875123T3 (enExample) |
| ES (1) | ES2717278T3 (enExample) |
| HR (1) | HRP20190464T1 (enExample) |
| IL (1) | IL236447B (enExample) |
| IN (1) | IN2014DN11263A (enExample) |
| LT (1) | LT2875123T (enExample) |
| MX (1) | MX366586B (enExample) |
| PL (1) | PL2875123T3 (enExample) |
| PT (1) | PT2875123T (enExample) |
| SI (1) | SI2875123T1 (enExample) |
| TR (1) | TR201903915T4 (enExample) |
| WO (1) | WO2014013045A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3271453B1 (en) | 2015-03-17 | 2021-09-08 | eleva GmbH | Glycosylated lysosomal proteins, method of production and uses |
| US12285513B2 (en) | 2015-04-14 | 2025-04-29 | Vytrus Biotech, S.L. | Cell-free plant cell culture suspension supernatant with re-youth activity and/or wound healing activity over skin cells |
| BE1025089B1 (fr) * | 2017-03-30 | 2018-10-29 | Univercells Sa | Clarification de culture cellulaire |
| WO2025147484A1 (en) * | 2024-01-04 | 2025-07-10 | Wainamics, Inc. | Microfluidic biospecimen cartridge |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1571504A (en) * | 1976-01-29 | 1980-07-16 | English Clays Lovering Pochin | Separation of solids and liquids |
| US5254096A (en) * | 1992-09-23 | 1993-10-19 | Becton, Dickinson And Company | Syringe pump with graphical display or error conditions |
| JP2000279772A (ja) * | 1999-03-30 | 2000-10-10 | Ngk Insulators Ltd | セラミック膜フィルターによるろ過方法 |
| DE19937187A1 (de) * | 1999-08-06 | 2001-03-01 | Qiagen Gmbh | Automatisierte Proteinreinigung im Multiwellformat durch Vakuumfiltration |
| US6692702B1 (en) * | 2000-07-07 | 2004-02-17 | Coulter International Corp. | Apparatus for biological sample preparation and analysis |
| EP1747278A1 (en) * | 2004-05-07 | 2007-01-31 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Method for producing cyclic peptides from in vitro plant cell cultures |
| EP2054498A1 (de) * | 2006-08-15 | 2009-05-06 | Basf Se | Verfahren zur isolierung von proteinen aus produktionszellen |
| US9932620B2 (en) * | 2012-05-21 | 2018-04-03 | Celsis Ltd. | Methods, devices, and systems of detecting microorganisms |
| JP6453592B2 (ja) * | 2013-09-25 | 2019-01-16 | アークレイ株式会社 | 血液検体の処理方法 |
-
2012
- 2012-07-20 EP EP12177277.6A patent/EP2687592A1/de not_active Withdrawn
-
2013
- 2013-07-19 BR BR112015001120-9A patent/BR112015001120B1/pt active IP Right Grant
- 2013-07-19 KR KR1020157001096A patent/KR102087258B1/ko active Active
- 2013-07-19 DK DK13739224.7T patent/DK2875123T3/en active
- 2013-07-19 HR HRP20190464TT patent/HRP20190464T1/hr unknown
- 2013-07-19 AU AU2013291990A patent/AU2013291990B2/en active Active
- 2013-07-19 LT LTEP13739224.7T patent/LT2875123T/lt unknown
- 2013-07-19 IN IN11263DEN2014 patent/IN2014DN11263A/en unknown
- 2013-07-19 EP EP13739224.7A patent/EP2875123B1/de active Active
- 2013-07-19 TR TR2019/03915T patent/TR201903915T4/tr unknown
- 2013-07-19 PL PL13739224T patent/PL2875123T3/pl unknown
- 2013-07-19 ES ES13739224T patent/ES2717278T3/es active Active
- 2013-07-19 SI SI201331390T patent/SI2875123T1/sl unknown
- 2013-07-19 CA CA2882100A patent/CA2882100C/en active Active
- 2013-07-19 MX MX2015000680A patent/MX366586B/es active IP Right Grant
- 2013-07-19 US US14/414,261 patent/US10081789B2/en active Active
- 2013-07-19 CN CN201380038651.3A patent/CN104769102B/zh active Active
- 2013-07-19 WO PCT/EP2013/065264 patent/WO2014013045A1/de not_active Ceased
- 2013-07-19 JP JP2015522110A patent/JP6693743B2/ja active Active
- 2013-07-19 PT PT13739224T patent/PT2875123T/pt unknown
-
2014
- 2014-12-25 IL IL236447A patent/IL236447B/en active IP Right Grant
-
2018
- 2018-06-13 JP JP2018112570A patent/JP2018157827A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL236447B (en) | 2019-05-30 |
| IN2014DN11263A (enExample) | 2015-10-09 |
| JP2018157827A (ja) | 2018-10-11 |
| MX366586B (es) | 2019-07-15 |
| DK2875123T3 (en) | 2019-04-08 |
| EP2875123B1 (de) | 2018-12-26 |
| SI2875123T1 (sl) | 2019-05-31 |
| CA2882100A1 (en) | 2014-01-23 |
| EP2875123A1 (de) | 2015-05-27 |
| AU2013291990B2 (en) | 2019-01-03 |
| KR20150031292A (ko) | 2015-03-23 |
| BR112015001120A2 (pt) | 2017-06-27 |
| CN104769102B (zh) | 2020-11-03 |
| BR112015001120B1 (pt) | 2022-03-22 |
| AU2013291990A1 (en) | 2015-01-29 |
| ES2717278T3 (es) | 2019-06-20 |
| KR102087258B1 (ko) | 2020-03-11 |
| MX2015000680A (es) | 2015-04-10 |
| PT2875123T (pt) | 2019-04-22 |
| PL2875123T3 (pl) | 2019-06-28 |
| IL236447A0 (en) | 2015-02-26 |
| TR201903915T4 (tr) | 2019-04-22 |
| HRP20190464T1 (hr) | 2019-05-03 |
| CN104769102A (zh) | 2015-07-08 |
| US10081789B2 (en) | 2018-09-25 |
| WO2014013045A1 (de) | 2014-01-23 |
| JP2015522287A (ja) | 2015-08-06 |
| EP2687592A1 (de) | 2014-01-22 |
| LT2875123T (lt) | 2019-04-10 |
| CA2882100C (en) | 2021-07-06 |
| US20150147811A1 (en) | 2015-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109689191B (zh) | 交替切向流快速收获 | |
| JP2018157827A (ja) | 細胞培養上清の濾過 | |
| KR20090021329A (ko) | 간엽 줄기세포의 배양 방법 | |
| CN106520666A (zh) | 马铃薯原生质体的高效分离、转化及再生的方法及其专用培养基 | |
| CN111690053B (zh) | 一种螺旋藻生物活性物质分级分离方法 | |
| JP2022182360A (ja) | 細胞外小胞の分離精製方法 | |
| CN107267549B (zh) | 一种中山杉品种406叶肉原生质体分离、纯化及高效转化的方法 | |
| NO310472B1 (no) | Biomasse og fremgangsmåte for fremstilling av virus/virusantigen | |
| KR102079633B1 (ko) | 식물 세포 팩의 생성 및 배양 방법 | |
| CN113817608A (zh) | 一种醇化烟叶表面微生物的分离和富集方法 | |
| CN112852728A (zh) | 基于外周血的lcl-nk细胞联合培养方法、细胞及产品 | |
| WO2005108596A1 (en) | Method for producing cyclic peptides from in vitro plant cell cultures | |
| JPWO2004058936A1 (ja) | 細胞の分離回収装置および分離回収方法 | |
| CN115475181A (zh) | 羊膜间充质干细胞源外泌体及其用途 | |
| WO2004076671A1 (fr) | Procede permettant de construire des algues economiques transgeniques | |
| CN102191226A (zh) | 细胞培养混合物的分离纯化方法 | |
| CN113913397A (zh) | 一种溶瘤病毒的纯化方法 | |
| CN114807011B (zh) | 一种暗纹东方鲀精巢细胞系及其构建方法和应用 | |
| CN118853555A (zh) | 一种脐带源间充质干细胞外泌体制备方法及应用 | |
| CN104592372A (zh) | 一种悬浮培养发菜糖蛋白的制备方法 | |
| JP2025009712A (ja) | 植物由来抽出液から植物由来可溶性タンパク質を除去する方法 | |
| CN118909935A (zh) | 一种大规模分离外泌体的方法及制备的外泌体和应用 | |
| CN119799632A (zh) | 一种功能增强的间充质干细胞外泌体的制备方法及其应用 | |
| Chieh Chan et al. | Improvement of biomass and juvenile hormone III (JH III) production fromCyperus aromaticuscell suspension culture via in situ membrane filtration technology | |
| TW201512398A (zh) | 自組織獲得高純度幹細胞之方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160624 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170523 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170822 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20180213 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20180615 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20190826 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20191108 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200131 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20200416 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6693743 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |