ES2717278T3 - Filtración de sobrenadantes de cultivos celulares - Google Patents
Filtración de sobrenadantes de cultivos celulares Download PDFInfo
- Publication number
- ES2717278T3 ES2717278T3 ES13739224T ES13739224T ES2717278T3 ES 2717278 T3 ES2717278 T3 ES 2717278T3 ES 13739224 T ES13739224 T ES 13739224T ES 13739224 T ES13739224 T ES 13739224T ES 2717278 T3 ES2717278 T3 ES 2717278T3
- Authority
- ES
- Spain
- Prior art keywords
- filter
- cells
- pore size
- bar
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001914 filtration Methods 0.000 title claims description 45
- 238000004113 cell culture Methods 0.000 title description 9
- 239000012228 culture supernatant Substances 0.000 title description 8
- 210000004027 cell Anatomy 0.000 claims description 62
- 238000000034 method Methods 0.000 claims description 39
- 241000196324 Embryophyta Species 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 29
- 239000011148 porous material Substances 0.000 claims description 29
- 239000012065 filter cake Substances 0.000 claims description 21
- 239000006228 supernatant Substances 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 12
- 230000008569 process Effects 0.000 claims description 9
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 5
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 5
- 210000002421 cell wall Anatomy 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 241000195940 Bryophyta Species 0.000 claims description 3
- 241000195887 Physcomitrella patens Species 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 description 19
- 239000000706 filtrate Substances 0.000 description 12
- 238000000746 purification Methods 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 10
- 102000004169 proteins and genes Human genes 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000002028 Biomass Substances 0.000 description 5
- 238000003828 vacuum filtration Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 4
- 238000011146 sterile filtration Methods 0.000 description 4
- 244000138620 Talinum patens Species 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000009261 transgenic effect Effects 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 241000339550 Landoltia Species 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000011118 depth filtration Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 210000002977 intracellular fluid Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920005597 polymer membrane Polymers 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010022366 Carcinoembryonic Antigen Proteins 0.000 description 1
- 241000195898 Ceratodon Species 0.000 description 1
- 241000398616 Chloronema Species 0.000 description 1
- 102000001189 Cyclic Peptides Human genes 0.000 description 1
- 108010069514 Cyclic Peptides Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241001148462 Funaria <moss> Species 0.000 description 1
- 101000737574 Homo sapiens Complement factor H Proteins 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 241000209499 Lemna Species 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 241000196322 Marchantia Species 0.000 description 1
- 241000196323 Marchantiophyta Species 0.000 description 1
- 244000107288 Meyna grisea Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000195888 Physcomitrella Species 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000736285 Sphagnum Species 0.000 description 1
- 241000339989 Wolffia Species 0.000 description 1
- 241000340053 Wolffiella Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000011138 biotechnological process Methods 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000009295 crossflow filtration Methods 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 102000045512 human CFH Human genes 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 210000004738 parenchymal cell Anatomy 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000011085 pressure filtration Methods 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 210000004233 talus Anatomy 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12177277.6A EP2687592A1 (de) | 2012-07-20 | 2012-07-20 | Filtration von Zellkulturüberständen |
| PCT/EP2013/065264 WO2014013045A1 (de) | 2012-07-20 | 2013-07-19 | Filtration von zellkulturüberständen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2717278T3 true ES2717278T3 (es) | 2019-06-20 |
Family
ID=48803559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13739224T Active ES2717278T3 (es) | 2012-07-20 | 2013-07-19 | Filtración de sobrenadantes de cultivos celulares |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US10081789B2 (enExample) |
| EP (2) | EP2687592A1 (enExample) |
| JP (2) | JP6693743B2 (enExample) |
| KR (1) | KR102087258B1 (enExample) |
| CN (1) | CN104769102B (enExample) |
| AU (1) | AU2013291990B2 (enExample) |
| BR (1) | BR112015001120B1 (enExample) |
| CA (1) | CA2882100C (enExample) |
| DK (1) | DK2875123T3 (enExample) |
| ES (1) | ES2717278T3 (enExample) |
| HR (1) | HRP20190464T1 (enExample) |
| IL (1) | IL236447B (enExample) |
| IN (1) | IN2014DN11263A (enExample) |
| LT (1) | LT2875123T (enExample) |
| MX (1) | MX366586B (enExample) |
| PL (1) | PL2875123T3 (enExample) |
| PT (1) | PT2875123T (enExample) |
| SI (1) | SI2875123T1 (enExample) |
| TR (1) | TR201903915T4 (enExample) |
| WO (1) | WO2014013045A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3271453B1 (en) | 2015-03-17 | 2021-09-08 | eleva GmbH | Glycosylated lysosomal proteins, method of production and uses |
| US12285513B2 (en) | 2015-04-14 | 2025-04-29 | Vytrus Biotech, S.L. | Cell-free plant cell culture suspension supernatant with re-youth activity and/or wound healing activity over skin cells |
| BE1025089B1 (fr) * | 2017-03-30 | 2018-10-29 | Univercells Sa | Clarification de culture cellulaire |
| WO2025147484A1 (en) * | 2024-01-04 | 2025-07-10 | Wainamics, Inc. | Microfluidic biospecimen cartridge |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1571504A (en) * | 1976-01-29 | 1980-07-16 | English Clays Lovering Pochin | Separation of solids and liquids |
| US5254096A (en) * | 1992-09-23 | 1993-10-19 | Becton, Dickinson And Company | Syringe pump with graphical display or error conditions |
| JP2000279772A (ja) * | 1999-03-30 | 2000-10-10 | Ngk Insulators Ltd | セラミック膜フィルターによるろ過方法 |
| DE19937187A1 (de) * | 1999-08-06 | 2001-03-01 | Qiagen Gmbh | Automatisierte Proteinreinigung im Multiwellformat durch Vakuumfiltration |
| US6692702B1 (en) * | 2000-07-07 | 2004-02-17 | Coulter International Corp. | Apparatus for biological sample preparation and analysis |
| EP1747278A1 (en) * | 2004-05-07 | 2007-01-31 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Method for producing cyclic peptides from in vitro plant cell cultures |
| EP2054498A1 (de) * | 2006-08-15 | 2009-05-06 | Basf Se | Verfahren zur isolierung von proteinen aus produktionszellen |
| US9932620B2 (en) * | 2012-05-21 | 2018-04-03 | Celsis Ltd. | Methods, devices, and systems of detecting microorganisms |
| JP6453592B2 (ja) * | 2013-09-25 | 2019-01-16 | アークレイ株式会社 | 血液検体の処理方法 |
-
2012
- 2012-07-20 EP EP12177277.6A patent/EP2687592A1/de not_active Withdrawn
-
2013
- 2013-07-19 BR BR112015001120-9A patent/BR112015001120B1/pt active IP Right Grant
- 2013-07-19 KR KR1020157001096A patent/KR102087258B1/ko active Active
- 2013-07-19 DK DK13739224.7T patent/DK2875123T3/en active
- 2013-07-19 HR HRP20190464TT patent/HRP20190464T1/hr unknown
- 2013-07-19 AU AU2013291990A patent/AU2013291990B2/en active Active
- 2013-07-19 LT LTEP13739224.7T patent/LT2875123T/lt unknown
- 2013-07-19 IN IN11263DEN2014 patent/IN2014DN11263A/en unknown
- 2013-07-19 EP EP13739224.7A patent/EP2875123B1/de active Active
- 2013-07-19 TR TR2019/03915T patent/TR201903915T4/tr unknown
- 2013-07-19 PL PL13739224T patent/PL2875123T3/pl unknown
- 2013-07-19 ES ES13739224T patent/ES2717278T3/es active Active
- 2013-07-19 SI SI201331390T patent/SI2875123T1/sl unknown
- 2013-07-19 CA CA2882100A patent/CA2882100C/en active Active
- 2013-07-19 MX MX2015000680A patent/MX366586B/es active IP Right Grant
- 2013-07-19 US US14/414,261 patent/US10081789B2/en active Active
- 2013-07-19 CN CN201380038651.3A patent/CN104769102B/zh active Active
- 2013-07-19 WO PCT/EP2013/065264 patent/WO2014013045A1/de not_active Ceased
- 2013-07-19 JP JP2015522110A patent/JP6693743B2/ja active Active
- 2013-07-19 PT PT13739224T patent/PT2875123T/pt unknown
-
2014
- 2014-12-25 IL IL236447A patent/IL236447B/en active IP Right Grant
-
2018
- 2018-06-13 JP JP2018112570A patent/JP2018157827A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL236447B (en) | 2019-05-30 |
| IN2014DN11263A (enExample) | 2015-10-09 |
| JP2018157827A (ja) | 2018-10-11 |
| MX366586B (es) | 2019-07-15 |
| DK2875123T3 (en) | 2019-04-08 |
| EP2875123B1 (de) | 2018-12-26 |
| SI2875123T1 (sl) | 2019-05-31 |
| CA2882100A1 (en) | 2014-01-23 |
| EP2875123A1 (de) | 2015-05-27 |
| AU2013291990B2 (en) | 2019-01-03 |
| KR20150031292A (ko) | 2015-03-23 |
| BR112015001120A2 (pt) | 2017-06-27 |
| CN104769102B (zh) | 2020-11-03 |
| BR112015001120B1 (pt) | 2022-03-22 |
| AU2013291990A1 (en) | 2015-01-29 |
| KR102087258B1 (ko) | 2020-03-11 |
| MX2015000680A (es) | 2015-04-10 |
| PT2875123T (pt) | 2019-04-22 |
| PL2875123T3 (pl) | 2019-06-28 |
| IL236447A0 (en) | 2015-02-26 |
| TR201903915T4 (tr) | 2019-04-22 |
| HRP20190464T1 (hr) | 2019-05-03 |
| JP6693743B2 (ja) | 2020-05-13 |
| CN104769102A (zh) | 2015-07-08 |
| US10081789B2 (en) | 2018-09-25 |
| WO2014013045A1 (de) | 2014-01-23 |
| JP2015522287A (ja) | 2015-08-06 |
| EP2687592A1 (de) | 2014-01-22 |
| LT2875123T (lt) | 2019-04-10 |
| CA2882100C (en) | 2021-07-06 |
| US20150147811A1 (en) | 2015-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2717278T3 (es) | Filtración de sobrenadantes de cultivos celulares | |
| Larkin | Purification and viability determinations of plant protoplasts | |
| Edwards et al. | Isolation of mesophyll cells and bundle sheath cells from Digitaria sanguinalis (L.) Scop. leaves and a scanning microscopy study of the internal leaf cell morphology | |
| KR101170707B1 (ko) | 재생된 카사바 식물로부터 전분을 분리하는 방법 및 그로부터 분리된 전분 | |
| CN110499287A (zh) | 简易制备胎盘间充质干细胞外泌体的方法 | |
| CN103003413A (zh) | 富含蜡酯的裸藻属藻体的生产方法及蜡酯制造方法 | |
| CN106318875B (zh) | 一种蝉花的双向人工培养方法 | |
| WO2020081658A1 (en) | Integrated unicellular/filamentous algal production, harvesting and remediation system | |
| CN106520666A (zh) | 马铃薯原生质体的高效分离、转化及再生的方法及其专用培养基 | |
| JPH04503900A (ja) | フレーバー組成物およびその生産方法 | |
| CN107267549A (zh) | 一种中山杉品种406叶肉原生质体分离、纯化及高效转化的方法 | |
| CN105969652A (zh) | 模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 | |
| ES2644262T3 (es) | Método para la generación y cultivo de un bloque de células vegetales | |
| CN101358181A (zh) | 一种水稻根系木质部薄壁细胞原生质体的分离方法 | |
| JP2009118785A (ja) | 底生性微細藻類の培養方法およびこれに利用する培養装置 | |
| CN103239477A (zh) | 间充质干细胞、神经干细胞和诱导干细胞活性因子的分离与制备方法 | |
| CN113817608A (zh) | 一种醇化烟叶表面微生物的分离和富集方法 | |
| WO2005108596A1 (en) | Method for producing cyclic peptides from in vitro plant cell cultures | |
| US6420173B1 (en) | Method for mass production and storage of seedlings of Eleutherococcus senticosus (Siberian ginseng) through cell culturing | |
| CN106922525B (zh) | 一种通过弱光培养体系快速获得转基因植物的方法 | |
| RU2639566C1 (ru) | Каллусный штамм культивируемых клеток растения живучка туркестанская Ajuga turkestanica (Regel) Briq. в условиях in vitro - продуцент туркестерона | |
| JP2025009712A (ja) | 植物由来抽出液から植物由来可溶性タンパク質を除去する方法 | |
| WO2025005226A1 (ja) | 植物由来抽出液から植物由来可溶性タンパク質を除去する方法 | |
| CN120944964A (zh) | 中国菰ZlRc和ZlMYB1基因胚乳特异性表达在提高水稻种子类黄酮含量中的应用 | |
| RU2371477C2 (ru) | Способ выделения клеточных ядер из пыльников пшеницы |