CN105969652A - 模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 - Google Patents
模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 Download PDFInfo
- Publication number
- CN105969652A CN105969652A CN201610304161.9A CN201610304161A CN105969652A CN 105969652 A CN105969652 A CN 105969652A CN 201610304161 A CN201610304161 A CN 201610304161A CN 105969652 A CN105969652 A CN 105969652A
- Authority
- CN
- China
- Prior art keywords
- culture
- bag filter
- plant
- suspension cell
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 66
- 239000000725 suspension Substances 0.000 title claims abstract description 62
- 238000004088 simulation Methods 0.000 title abstract description 4
- 230000002503 metabolic effect Effects 0.000 title abstract description 3
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 238000004458 analytical method Methods 0.000 claims abstract description 8
- 238000003501 co-culture Methods 0.000 claims description 37
- 241000894006 Bacteria Species 0.000 claims description 12
- 230000033228 biological regulation Effects 0.000 claims description 9
- 239000002207 metabolite Substances 0.000 claims description 7
- 238000003780 insertion Methods 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims description 5
- 238000002955 isolation Methods 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 4
- 239000004677 Nylon Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 229920001778 nylon Polymers 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 14
- 230000008569 process Effects 0.000 abstract description 5
- 238000000338 in vitro Methods 0.000 abstract description 3
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract description 2
- 238000000502 dialysis Methods 0.000 abstract 5
- 238000007789 sealing Methods 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 66
- 241000196324 Embryophyta Species 0.000 description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- ZOOGRGPOEVQQDX-KHLHZJAASA-N cyclic guanosine monophosphate Chemical compound C([C@H]1O2)O[P@](O)(=O)O[C@@H]1[C@H](O)[C@H]2N1C(N=C(NC2=O)N)=C2N=C1 ZOOGRGPOEVQQDX-KHLHZJAASA-N 0.000 description 18
- 102000000584 Calmodulin Human genes 0.000 description 15
- 108010041952 Calmodulin Proteins 0.000 description 15
- 238000001514 detection method Methods 0.000 description 15
- 230000001954 sterilising effect Effects 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 238000002156 mixing Methods 0.000 description 12
- 239000010413 mother solution Substances 0.000 description 12
- 230000004060 metabolic process Effects 0.000 description 11
- 206010020649 Hyperkeratosis Diseases 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 238000010790 dilution Methods 0.000 description 9
- 239000012895 dilution Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 238000003753 real-time PCR Methods 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 230000014509 gene expression Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 101100381451 Panax ginseng OSCPNY1 gene Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 5
- 102000004286 Hydroxymethylglutaryl CoA Reductases Human genes 0.000 description 5
- 108090000895 Hydroxymethylglutaryl CoA Reductases Proteins 0.000 description 5
- 108010017796 epoxidase Proteins 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 108700005078 Synthetic Genes Proteins 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 230000000857 drug effect Effects 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 239000004570 mortar (masonry) Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 108091092584 GDNA Proteins 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 229960004756 ethanol Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 230000007412 host metabolism Effects 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 101150044508 key gene Proteins 0.000 description 3
- 229960002523 mercuric chloride Drugs 0.000 description 3
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 102000007469 Actins Human genes 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000223602 Alternaria alternata Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000213015 Bupleurum scorzonerifolium Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 150000002505 iron Chemical class 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229930000044 secondary metabolite Natural products 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 238000003828 vacuum filtration Methods 0.000 description 2
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 1
- VOZKAJLKRJDJLL-UHFFFAOYSA-N 2,4-diaminotoluene Chemical compound CC1=CC=C(N)C=C1N VOZKAJLKRJDJLL-UHFFFAOYSA-N 0.000 description 1
- VWFJDQUYCIWHTN-YFVJMOTDSA-N 2-trans,6-trans-farnesyl diphosphate Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CO[P@](O)(=O)OP(O)(O)=O VWFJDQUYCIWHTN-YFVJMOTDSA-N 0.000 description 1
- LMYZQUNLYGJIHI-SPONXPENSA-N 4alpha-methyl-5alpha-cholest-7-en-3beta-ol Chemical compound C[C@@H]1[C@@H](O)CC[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@H](C)CCCC(C)C)CC[C@H]33)C)C3=CC[C@H]21 LMYZQUNLYGJIHI-SPONXPENSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 208000035126 Facies Diseases 0.000 description 1
- VWFJDQUYCIWHTN-UHFFFAOYSA-N Farnesyl pyrophosphate Natural products CC(C)=CCCC(C)=CCCC(C)=CCOP(O)(=O)OP(O)(O)=O VWFJDQUYCIWHTN-UHFFFAOYSA-N 0.000 description 1
- 108010022535 Farnesyl-Diphosphate Farnesyltransferase Proteins 0.000 description 1
- 101150089429 HMGR gene Proteins 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- NUHSROFQTUXZQQ-UHFFFAOYSA-N Isopentenyl diphosphate Natural products CC(=C)CCO[P@](O)(=O)OP(O)(O)=O NUHSROFQTUXZQQ-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010806 PrimeScriptTM RT Reagent kit Methods 0.000 description 1
- 102000005782 Squalene Monooxygenase Human genes 0.000 description 1
- 108020003891 Squalene monooxygenase Proteins 0.000 description 1
- 102100037997 Squalene synthase Human genes 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- FSLPMRQHCOLESF-UHFFFAOYSA-N alpha-amyrenol Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C)CCC(C)C(C)C5C4=CCC3C21C FSLPMRQHCOLESF-UHFFFAOYSA-N 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- JFSHUTJDVKUMTJ-QHPUVITPSA-N beta-amyrin Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C JFSHUTJDVKUMTJ-QHPUVITPSA-N 0.000 description 1
- QQFMRPIKDLHLKB-UHFFFAOYSA-N beta-amyrin Natural products CC1C2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C)CCC1(C)C QQFMRPIKDLHLKB-UHFFFAOYSA-N 0.000 description 1
- PDNLMONKODEGSE-UHFFFAOYSA-N beta-amyrin acetate Natural products CC(=O)OC1CCC2(C)C(CCC3(C)C4(C)CCC5(C)CCC(C)(C)CC5C4=CCC23C)C1(C)C PDNLMONKODEGSE-UHFFFAOYSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 239000011538 cleaning material Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229930182493 triterpene saponin Natural products 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Physiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610304161.9A CN105969652B (zh) | 2016-05-10 | 2016-05-10 | 模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610304161.9A CN105969652B (zh) | 2016-05-10 | 2016-05-10 | 模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105969652A true CN105969652A (zh) | 2016-09-28 |
CN105969652B CN105969652B (zh) | 2019-11-19 |
Family
ID=56991479
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610304161.9A Active CN105969652B (zh) | 2016-05-10 | 2016-05-10 | 模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105969652B (zh) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109504605A (zh) * | 2018-11-01 | 2019-03-22 | 深圳市华唐兴东生物科技有限公司 | 细胞共培养方法、装置及其用途 |
CN110186735A (zh) * | 2019-07-02 | 2019-08-30 | 齐齐哈尔大学 | 一种含类细胞质人造细胞的制备方法 |
CN110484439A (zh) * | 2019-08-19 | 2019-11-22 | 河北经贸大学 | 一种筛选生防菌的装置及筛选生防菌的方法 |
CN113980885A (zh) * | 2021-12-07 | 2022-01-28 | 西南科技大学 | 一种柴胡原生质体的制备及瞬时转化方法 |
CN115820430A (zh) * | 2022-09-26 | 2023-03-21 | 浙江中医药大学 | 一种白术内生真菌及其在白术悬浮细胞培养中的应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102851213A (zh) * | 2012-08-23 | 2013-01-02 | 王培磊 | 绿色巴夫藻培养基配方及三级培养方法 |
WO2013148511A1 (en) * | 2012-03-24 | 2013-10-03 | Therapeutic Proteins International, LLC | Pivoting pressurized single-use bioreactor |
CN204237802U (zh) * | 2014-11-11 | 2015-04-01 | 人和未来生物科技(长沙)有限公司 | 非接触式细胞共培养装置 |
CN105296416A (zh) * | 2015-12-02 | 2016-02-03 | 王振月 | 毛脉酸模悬浮细胞与其内生真菌共培养体系建立的方法 |
-
2016
- 2016-05-10 CN CN201610304161.9A patent/CN105969652B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013148511A1 (en) * | 2012-03-24 | 2013-10-03 | Therapeutic Proteins International, LLC | Pivoting pressurized single-use bioreactor |
CN102851213A (zh) * | 2012-08-23 | 2013-01-02 | 王培磊 | 绿色巴夫藻培养基配方及三级培养方法 |
CN204237802U (zh) * | 2014-11-11 | 2015-04-01 | 人和未来生物科技(长沙)有限公司 | 非接触式细胞共培养装置 |
CN105296416A (zh) * | 2015-12-02 | 2016-02-03 | 王振月 | 毛脉酸模悬浮细胞与其内生真菌共培养体系建立的方法 |
Non-Patent Citations (1)
Title |
---|
唐克轩主编: "《中草药生物技术》", 30 June 2005, 复旦大学出版社 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109504605A (zh) * | 2018-11-01 | 2019-03-22 | 深圳市华唐兴东生物科技有限公司 | 细胞共培养方法、装置及其用途 |
CN110186735A (zh) * | 2019-07-02 | 2019-08-30 | 齐齐哈尔大学 | 一种含类细胞质人造细胞的制备方法 |
CN110186735B (zh) * | 2019-07-02 | 2021-07-27 | 齐齐哈尔大学 | 一种含类细胞质人造细胞的制备方法 |
CN110484439A (zh) * | 2019-08-19 | 2019-11-22 | 河北经贸大学 | 一种筛选生防菌的装置及筛选生防菌的方法 |
CN110484439B (zh) * | 2019-08-19 | 2023-04-07 | 河北经贸大学 | 一种筛选生防菌的装置及筛选生防菌的方法 |
CN113980885A (zh) * | 2021-12-07 | 2022-01-28 | 西南科技大学 | 一种柴胡原生质体的制备及瞬时转化方法 |
CN115820430A (zh) * | 2022-09-26 | 2023-03-21 | 浙江中医药大学 | 一种白术内生真菌及其在白术悬浮细胞培养中的应用 |
CN115820430B (zh) * | 2022-09-26 | 2024-04-26 | 浙江中医药大学 | 一种白术内生真菌及其在白术悬浮细胞培养中的应用 |
Also Published As
Publication number | Publication date |
---|---|
CN105969652B (zh) | 2019-11-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Senanayake et al. | Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation | |
Howieson et al. | Working with rhizobia | |
CN105969652A (zh) | 模拟植物悬浮细胞与其内生真菌代谢交流的共培养装置 | |
CN105296381B (zh) | 一株枯草芽孢杆菌cyy-25及其应用 | |
CN104651239B (zh) | 一种促进齿瓣石斛种子萌发形成幼苗的菌株及其应用 | |
CN105886405B (zh) | 铁皮石斛内生真菌及其应用 | |
CN101654665B (zh) | 一种制备枯草芽孢杆菌的方法 | |
CN106011005A (zh) | 一种解淀粉芽孢杆菌t600及其菌剂制备方法和应用 | |
CN105586274A (zh) | 拟康宁木霉t-51菌株及其在番茄促生和灰霉病生物防治的应用 | |
Pető et al. | Macro-and micro-archaeobotanical study of a vessel content from a Late Neolithic structured deposition from southeastern Hungary | |
CN104745672A (zh) | 一种快速鉴定烟草黑胫病抗性的方法 | |
CN109452088A (zh) | 金针菇x18及其栽培方法 | |
CN109136137A (zh) | 一种抗重金属的植物促生菌株及其应用 | |
CN109880757A (zh) | 一株具有自身固氮能力的氢氧化细菌及其分离方法和应用 | |
CN102925387B (zh) | 一株具诱导大豆抗大豆胞囊线虫的简单芽孢杆菌及应用 | |
CN106434490B (zh) | 人参防病促生细菌ty15-2及其应用 | |
CN109006000A (zh) | 一种鉴定筛选香蕉枯萎病抗耐病种苗的方法 | |
CN103333858A (zh) | Gleevec耐药的胃肠道间质瘤细胞系及其方法及裸鼠移植瘤模型 | |
CN107058120A (zh) | 对黄瓜根结线虫具强致病力的淡紫紫孢菌及其应用 | |
CN106434362A (zh) | 一株抗紫外线的高毒力金龟子绿僵菌诱变菌株MaUV‑1及其应用 | |
CN106613276A (zh) | 一种青蒿幼苗培育方法及其专用深绿木霉菌肥 | |
CN110004065A (zh) | 一种红褐灵芝新菌种及其人工栽培方法和应用 | |
CN106134728A (zh) | 一种利用根瘤菌促进非豆科作物生长的方法 | |
CN104198678A (zh) | 一套适合评价土壤中农药残留毒性变化的方法 | |
CN101781630B (zh) | 一种根瘤固氮菌株系ry1菌株及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20201111 Address after: 150040 Heping Road 24, Xiangfang District, Heilongjiang, Harbin Patentee after: HEILONGJIANG University OF CHINESE MEDICINE Address before: 150040 comprehensive building, School of pharmacy, Heilongjiang University Of Chinese Medicine, Heping Road, 24, Heilongjiang, Harbin 1616, Xiangfang Patentee before: Cheng Yupeng |
|
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20160928 Assignee: Heilongjiang Guanzhong traditional Chinese Medicine Technology Co.,Ltd. Assignor: HEILONGJIANG University OF CHINESE MEDICINE Contract record no.: X2024980000920 Denomination of invention: A Co culture Device for Simulating Metabolic Exchange between Plant Suspension Cells and Endophytic Fungi Granted publication date: 20191119 License type: Common License Record date: 20240117 |