MX2015000680A - Filtracion de sobrenadantes de cultivo celular. - Google Patents

Filtracion de sobrenadantes de cultivo celular.

Info

Publication number
MX2015000680A
MX2015000680A MX2015000680A MX2015000680A MX2015000680A MX 2015000680 A MX2015000680 A MX 2015000680A MX 2015000680 A MX2015000680 A MX 2015000680A MX 2015000680 A MX2015000680 A MX 2015000680A MX 2015000680 A MX2015000680 A MX 2015000680A
Authority
MX
Mexico
Prior art keywords
mixture
filter
filter housing
liquid
filtration
Prior art date
Application number
MX2015000680A
Other languages
English (en)
Other versions
MX366586B (es
Inventor
Andreas Schaaf
Thomas Grosse
Holger Niederkrüger
Original Assignee
Greenovation Biotech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenovation Biotech Gmbh filed Critical Greenovation Biotech Gmbh
Publication of MX2015000680A publication Critical patent/MX2015000680A/es
Publication of MX366586B publication Critical patent/MX366586B/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02Separating microorganisms from the culture medium; Concentration of biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Water Supply & Treatment (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención se refiere a un método para la separación de un líquido sobrenadante de las células, que comprende los pasos de: proporcionar una mezcla de las células con un líquido, cargar una primera carcasa o alojamiento del filtro con la mezcla, en el que en la carcasa del filtro se proporciona un filtro que tiene un tamaño de poro de entre 4 µm y 50 µm sobre una superficie de base plana perforada en una manera tamiz y las paredes de la carcasa del filtro están conectadas con el fin de sellar con la superficie de base plana, que es perforado a manera tamiz, aplicando una presión diferencial de al menos 0.5 bar en la mezcla, como resultado de lo cual la porción líquida de la mezcla es forzada a través del filtro y una torta de filtro que contiene las células permanece en la carcasa del filtro, y la eliminación del líquido filtrado.
MX2015000680A 2012-07-20 2013-07-19 Filtracion de sobrenadantes de cultivo celular. MX366586B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12177277.6A EP2687592A1 (de) 2012-07-20 2012-07-20 Filtration von Zellkulturüberständen
PCT/EP2013/065264 WO2014013045A1 (de) 2012-07-20 2013-07-19 Filtration von zellkulturüberständen

Publications (2)

Publication Number Publication Date
MX2015000680A true MX2015000680A (es) 2015-04-10
MX366586B MX366586B (es) 2019-07-15

Family

ID=48803559

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015000680A MX366586B (es) 2012-07-20 2013-07-19 Filtracion de sobrenadantes de cultivo celular.

Country Status (20)

Country Link
US (1) US10081789B2 (es)
EP (2) EP2687592A1 (es)
JP (2) JP6693743B2 (es)
KR (1) KR102087258B1 (es)
CN (1) CN104769102B (es)
AU (1) AU2013291990B2 (es)
BR (1) BR112015001120B1 (es)
CA (1) CA2882100C (es)
DK (1) DK2875123T3 (es)
ES (1) ES2717278T3 (es)
HR (1) HRP20190464T1 (es)
IL (1) IL236447B (es)
IN (1) IN2014DN11263A (es)
LT (1) LT2875123T (es)
MX (1) MX366586B (es)
PL (1) PL2875123T3 (es)
PT (1) PT2875123T (es)
SI (1) SI2875123T1 (es)
TR (1) TR201903915T4 (es)
WO (1) WO2014013045A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2899783T3 (es) 2015-03-17 2022-03-14 eleva GmbH Proteínas lisosómicas glucosiladas, método de producción y utilizaciones
EP3283092A1 (en) * 2015-04-14 2018-02-21 Vytrus Biotech, S.L. Cell-free plant cell culture suspension supernatant with re-youth activity and/or wound healing activity over skin cells

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1571504A (en) * 1976-01-29 1980-07-16 English Clays Lovering Pochin Separation of solids and liquids
US5254096A (en) * 1992-09-23 1993-10-19 Becton, Dickinson And Company Syringe pump with graphical display or error conditions
JP2000279772A (ja) 1999-03-30 2000-10-10 Ngk Insulators Ltd セラミック膜フィルターによるろ過方法
DE19937187A1 (de) * 1999-08-06 2001-03-01 Qiagen Gmbh Automatisierte Proteinreinigung im Multiwellformat durch Vakuumfiltration
US6692702B1 (en) * 2000-07-07 2004-02-17 Coulter International Corp. Apparatus for biological sample preparation and analysis
EP1747278A1 (en) * 2004-05-07 2007-01-31 Friedrich-Alexander-Universität Erlangen-Nürnberg Method for producing cyclic peptides from in vitro plant cell cultures
EP2054498A1 (de) * 2006-08-15 2009-05-06 Basf Se Verfahren zur isolierung von proteinen aus produktionszellen
US9932620B2 (en) * 2012-05-21 2018-04-03 Celsis Ltd. Methods, devices, and systems of detecting microorganisms
JP6453592B2 (ja) * 2013-09-25 2019-01-16 アークレイ株式会社 血液検体の処理方法

Also Published As

Publication number Publication date
EP2687592A1 (de) 2014-01-22
AU2013291990A1 (en) 2015-01-29
TR201903915T4 (tr) 2019-04-22
AU2013291990B2 (en) 2019-01-03
WO2014013045A1 (de) 2014-01-23
LT2875123T (lt) 2019-04-10
CA2882100C (en) 2021-07-06
BR112015001120B1 (pt) 2022-03-22
JP2015522287A (ja) 2015-08-06
EP2875123A1 (de) 2015-05-27
ES2717278T3 (es) 2019-06-20
US20150147811A1 (en) 2015-05-28
US10081789B2 (en) 2018-09-25
MX366586B (es) 2019-07-15
CN104769102B (zh) 2020-11-03
SI2875123T1 (sl) 2019-05-31
IL236447A0 (en) 2015-02-26
KR20150031292A (ko) 2015-03-23
JP6693743B2 (ja) 2020-05-13
DK2875123T3 (en) 2019-04-08
KR102087258B1 (ko) 2020-03-11
HRP20190464T1 (hr) 2019-05-03
CA2882100A1 (en) 2014-01-23
IN2014DN11263A (es) 2015-10-09
BR112015001120A2 (pt) 2017-06-27
PL2875123T3 (pl) 2019-06-28
CN104769102A (zh) 2015-07-08
EP2875123B1 (de) 2018-12-26
IL236447B (en) 2019-05-30
PT2875123T (pt) 2019-04-22
JP2018157827A (ja) 2018-10-11

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