BR112015001120A2 - filtração de sobrenadantes de cultura de células - Google Patents

filtração de sobrenadantes de cultura de células

Info

Publication number
BR112015001120A2
BR112015001120A2 BR112015001120A BR112015001120A BR112015001120A2 BR 112015001120 A2 BR112015001120 A2 BR 112015001120A2 BR 112015001120 A BR112015001120 A BR 112015001120A BR 112015001120 A BR112015001120 A BR 112015001120A BR 112015001120 A2 BR112015001120 A2 BR 112015001120A2
Authority
BR
Brazil
Prior art keywords
filter
mixture
filtration
cell culture
culture supernatants
Prior art date
Application number
BR112015001120A
Other languages
English (en)
Other versions
BR112015001120B1 (pt
Inventor
Schaaf Andreas
Niederkrüger Holger
grosse Thomas
Original Assignee
Greenovation Biotech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenovation Biotech Gmbh filed Critical Greenovation Biotech Gmbh
Publication of BR112015001120A2 publication Critical patent/BR112015001120A2/pt
Publication of BR112015001120B1 publication Critical patent/BR112015001120B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Water Supply & Treatment (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

resumo da patente de invenção para "filtração de sobrenadantes de cultura de células". a invenção se refere a um processo para a separação de um sobrenadante líquido de células compreendendo as etapas de: fornecimento de uma mistura das células com um líquido, carregamento de um primeiro compartimento de filtro com a mistura, em que no compartimento de filtro é fornecido um filtro com um tamanho de poro entre 4 µm e 50 µm sobre uma superfície de base plana perfurada em forma de peneira e as paredes do compartimento de filtro são conectadas de forma a vedar com a superfície de base plana, que é perfurada em forma de peneira, aplicação de uma pressão diferencial de pelo menos 0,5 bar (50000 pa) sobre a mistura, como um resultado do qual a porção líquida da mistura é forçada através do filtro e um bolo de filtração contendo células permanece no compartimento de filtro, e remoção do fluido filtrado.
BR112015001120-9A 2012-07-20 2013-07-19 Filtração de sobrenadantes de cultura de células BR112015001120B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12177277.6A EP2687592A1 (de) 2012-07-20 2012-07-20 Filtration von Zellkulturüberständen
EP12177277.6 2012-07-20
PCT/EP2013/065264 WO2014013045A1 (de) 2012-07-20 2013-07-19 Filtration von zellkulturüberständen

Publications (2)

Publication Number Publication Date
BR112015001120A2 true BR112015001120A2 (pt) 2017-06-27
BR112015001120B1 BR112015001120B1 (pt) 2022-03-22

Family

ID=48803559

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015001120-9A BR112015001120B1 (pt) 2012-07-20 2013-07-19 Filtração de sobrenadantes de cultura de células

Country Status (20)

Country Link
US (1) US10081789B2 (pt)
EP (2) EP2687592A1 (pt)
JP (2) JP6693743B2 (pt)
KR (1) KR102087258B1 (pt)
CN (1) CN104769102B (pt)
AU (1) AU2013291990B2 (pt)
BR (1) BR112015001120B1 (pt)
CA (1) CA2882100C (pt)
DK (1) DK2875123T3 (pt)
ES (1) ES2717278T3 (pt)
HR (1) HRP20190464T1 (pt)
IL (1) IL236447B (pt)
IN (1) IN2014DN11263A (pt)
LT (1) LT2875123T (pt)
MX (1) MX366586B (pt)
PL (1) PL2875123T3 (pt)
PT (1) PT2875123T (pt)
SI (1) SI2875123T1 (pt)
TR (1) TR201903915T4 (pt)
WO (1) WO2014013045A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE056850T2 (hu) 2015-03-17 2022-03-28 eleva GmbH Glikozilezett lizoszómális fehérjék, elõállítási eljárás és alkalmazások
US20180133138A1 (en) * 2015-04-14 2018-05-17 Vytrus Biotech, S.L. Cell-free plant cell culture suspension supernatant with re-youth activity and/or wound healing activity over skin cells
BE1025089B1 (fr) * 2017-03-30 2018-10-29 Univercells Sa Clarification de culture cellulaire

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1571504A (en) * 1976-01-29 1980-07-16 English Clays Lovering Pochin Separation of solids and liquids
US5254096A (en) * 1992-09-23 1993-10-19 Becton, Dickinson And Company Syringe pump with graphical display or error conditions
JP2000279772A (ja) * 1999-03-30 2000-10-10 Ngk Insulators Ltd セラミック膜フィルターによるろ過方法
DE19937187A1 (de) * 1999-08-06 2001-03-01 Qiagen Gmbh Automatisierte Proteinreinigung im Multiwellformat durch Vakuumfiltration
US6692702B1 (en) * 2000-07-07 2004-02-17 Coulter International Corp. Apparatus for biological sample preparation and analysis
EP1747278A1 (en) * 2004-05-07 2007-01-31 Friedrich-Alexander-Universität Erlangen-Nürnberg Method for producing cyclic peptides from in vitro plant cell cultures
JP2010500042A (ja) * 2006-08-15 2010-01-07 ビーエーエスエフ ソシエタス・ヨーロピア 産生細胞からのタンパク質単離方法
US9932620B2 (en) * 2012-05-21 2018-04-03 Celsis Ltd. Methods, devices, and systems of detecting microorganisms
JP6453592B2 (ja) * 2013-09-25 2019-01-16 アークレイ株式会社 血液検体の処理方法

Also Published As

Publication number Publication date
EP2875123A1 (de) 2015-05-27
US10081789B2 (en) 2018-09-25
MX2015000680A (es) 2015-04-10
ES2717278T3 (es) 2019-06-20
AU2013291990B2 (en) 2019-01-03
IL236447A0 (en) 2015-02-26
KR102087258B1 (ko) 2020-03-11
AU2013291990A1 (en) 2015-01-29
LT2875123T (lt) 2019-04-10
PL2875123T3 (pl) 2019-06-28
US20150147811A1 (en) 2015-05-28
PT2875123T (pt) 2019-04-22
WO2014013045A1 (de) 2014-01-23
IL236447B (en) 2019-05-30
SI2875123T1 (sl) 2019-05-31
JP2018157827A (ja) 2018-10-11
DK2875123T3 (en) 2019-04-08
JP6693743B2 (ja) 2020-05-13
EP2875123B1 (de) 2018-12-26
MX366586B (es) 2019-07-15
TR201903915T4 (tr) 2019-04-22
CN104769102B (zh) 2020-11-03
BR112015001120B1 (pt) 2022-03-22
KR20150031292A (ko) 2015-03-23
IN2014DN11263A (pt) 2015-10-09
CA2882100A1 (en) 2014-01-23
CA2882100C (en) 2021-07-06
CN104769102A (zh) 2015-07-08
JP2015522287A (ja) 2015-08-06
HRP20190464T1 (hr) 2019-05-03
EP2687592A1 (de) 2014-01-22

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B25D Requested change of name of applicant approved

Owner name: ELEVA GMBH (DE)

B25G Requested change of headquarter approved

Owner name: ELEVA GMBH (DE)

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B25K Entry of change of name and/or headquarter and transfer of application, patent and certificate of addition of invention: republication

Owner name: ELEVA GMBH (DE)

Free format text: RETIFICACAO DO DESPACHO (25.7) ? ALTERACAO DE SEDE PUBLICADO NA RPI NO 2637, DE 20/07/2021, QUANTO AO ITEM (71) ? DEPOSITANTE NO PARECER.ONDE SE LE: GREENOVATION BIOTECH GMBHLEIA-SE: ELEVA GMBH

B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 19/07/2013, OBSERVADAS AS CONDICOES LEGAIS.