MX366586B - Filtracion de sobrenadantes de cultivo celular. - Google Patents
Filtracion de sobrenadantes de cultivo celular.Info
- Publication number
- MX366586B MX366586B MX2015000680A MX2015000680A MX366586B MX 366586 B MX366586 B MX 366586B MX 2015000680 A MX2015000680 A MX 2015000680A MX 2015000680 A MX2015000680 A MX 2015000680A MX 366586 B MX366586 B MX 366586B
- Authority
- MX
- Mexico
- Prior art keywords
- mixture
- filter
- filter housing
- liquid
- filtration
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un método para separar un sobrenadante líquido a partir de células que tienen una pared celular, caracterizado porque comprende las etapas de: a) proporcionar una mezcla de las células con un líquido, b) llenar una carcasa de filtro con la mezcla, en donde se proporciona un primer filtro que tiene un tamaño de poro de entre 4 µm a 50 µm sobre una superficie de base plana en la carcasa del filtro y las paredes de la carcasa del filtro se conectan de manera sellada con la superficie de base plana, que está perforada en una forma tipo tamiz; c) aplicar una presión diferencial de al menos 50 kPa (0.5 bares) a la mezcla por medio de aire comprimido, como un resultado de lo cual se presiona la porción líquida de la mezcla a través del filtro y una torta de filtro que contiene células permanece en la carcasa del filtro, en donde la presión absoluta en el punto de extracción es al menos 70 kPa (0.7 bares); d) extraer el líquido filtrado.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12177277.6A EP2687592A1 (de) | 2012-07-20 | 2012-07-20 | Filtration von Zellkulturüberständen |
| PCT/EP2013/065264 WO2014013045A1 (de) | 2012-07-20 | 2013-07-19 | Filtration von zellkulturüberständen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2015000680A MX2015000680A (es) | 2015-04-10 |
| MX366586B true MX366586B (es) | 2019-07-15 |
Family
ID=48803559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015000680A MX366586B (es) | 2012-07-20 | 2013-07-19 | Filtracion de sobrenadantes de cultivo celular. |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US10081789B2 (es) |
| EP (2) | EP2687592A1 (es) |
| JP (2) | JP6693743B2 (es) |
| KR (1) | KR102087258B1 (es) |
| CN (1) | CN104769102B (es) |
| AU (1) | AU2013291990B2 (es) |
| BR (1) | BR112015001120B1 (es) |
| CA (1) | CA2882100C (es) |
| DK (1) | DK2875123T3 (es) |
| ES (1) | ES2717278T3 (es) |
| HR (1) | HRP20190464T1 (es) |
| IL (1) | IL236447B (es) |
| IN (1) | IN2014DN11263A (es) |
| LT (1) | LT2875123T (es) |
| MX (1) | MX366586B (es) |
| PL (1) | PL2875123T3 (es) |
| PT (1) | PT2875123T (es) |
| SI (1) | SI2875123T1 (es) |
| TR (1) | TR201903915T4 (es) |
| WO (1) | WO2014013045A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016146760A1 (en) | 2015-03-17 | 2016-09-22 | Greenovation Biotech Gmbh | Glycosylated lysosomal proteins, method of production and uses |
| EP3283092A1 (en) * | 2015-04-14 | 2018-02-21 | Vytrus Biotech, S.L. | Cell-free plant cell culture suspension supernatant with re-youth activity and/or wound healing activity over skin cells |
| BE1025089B1 (fr) * | 2017-03-30 | 2018-10-29 | Univercells Sa | Clarification de culture cellulaire |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1571504A (en) * | 1976-01-29 | 1980-07-16 | English Clays Lovering Pochin | Separation of solids and liquids |
| US5254096A (en) * | 1992-09-23 | 1993-10-19 | Becton, Dickinson And Company | Syringe pump with graphical display or error conditions |
| JP2000279772A (ja) | 1999-03-30 | 2000-10-10 | Ngk Insulators Ltd | セラミック膜フィルターによるろ過方法 |
| DE19937187A1 (de) * | 1999-08-06 | 2001-03-01 | Qiagen Gmbh | Automatisierte Proteinreinigung im Multiwellformat durch Vakuumfiltration |
| US6692702B1 (en) * | 2000-07-07 | 2004-02-17 | Coulter International Corp. | Apparatus for biological sample preparation and analysis |
| WO2005108596A1 (en) * | 2004-05-07 | 2005-11-17 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Method for producing cyclic peptides from in vitro plant cell cultures |
| US20090233348A1 (en) * | 2006-08-15 | 2009-09-17 | Basf Se | Method for isolating proteins from production cells |
| US9932620B2 (en) * | 2012-05-21 | 2018-04-03 | Celsis Ltd. | Methods, devices, and systems of detecting microorganisms |
| JP6453592B2 (ja) * | 2013-09-25 | 2019-01-16 | アークレイ株式会社 | 血液検体の処理方法 |
-
2012
- 2012-07-20 EP EP12177277.6A patent/EP2687592A1/de not_active Withdrawn
-
2013
- 2013-07-19 PT PT13739224T patent/PT2875123T/pt unknown
- 2013-07-19 JP JP2015522110A patent/JP6693743B2/ja active Active
- 2013-07-19 CA CA2882100A patent/CA2882100C/en active Active
- 2013-07-19 KR KR1020157001096A patent/KR102087258B1/ko active IP Right Grant
- 2013-07-19 IN IN11263DEN2014 patent/IN2014DN11263A/en unknown
- 2013-07-19 EP EP13739224.7A patent/EP2875123B1/de active Active
- 2013-07-19 BR BR112015001120-9A patent/BR112015001120B1/pt active IP Right Grant
- 2013-07-19 MX MX2015000680A patent/MX366586B/es active IP Right Grant
- 2013-07-19 CN CN201380038651.3A patent/CN104769102B/zh active Active
- 2013-07-19 AU AU2013291990A patent/AU2013291990B2/en active Active
- 2013-07-19 WO PCT/EP2013/065264 patent/WO2014013045A1/de active Application Filing
- 2013-07-19 PL PL13739224T patent/PL2875123T3/pl unknown
- 2013-07-19 LT LTEP13739224.7T patent/LT2875123T/lt unknown
- 2013-07-19 SI SI201331390T patent/SI2875123T1/sl unknown
- 2013-07-19 US US14/414,261 patent/US10081789B2/en active Active
- 2013-07-19 ES ES13739224T patent/ES2717278T3/es active Active
- 2013-07-19 TR TR2019/03915T patent/TR201903915T4/tr unknown
- 2013-07-19 DK DK13739224.7T patent/DK2875123T3/en active
-
2014
- 2014-12-25 IL IL236447A patent/IL236447B/en active IP Right Grant
-
2018
- 2018-06-13 JP JP2018112570A patent/JP2018157827A/ja active Pending
-
2019
- 2019-03-08 HR HRP20190464TT patent/HRP20190464T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL236447B (en) | 2019-05-30 |
| EP2687592A1 (de) | 2014-01-22 |
| EP2875123A1 (de) | 2015-05-27 |
| PL2875123T3 (pl) | 2019-06-28 |
| EP2875123B1 (de) | 2018-12-26 |
| KR20150031292A (ko) | 2015-03-23 |
| JP2018157827A (ja) | 2018-10-11 |
| CA2882100C (en) | 2021-07-06 |
| TR201903915T4 (tr) | 2019-04-22 |
| BR112015001120B1 (pt) | 2022-03-22 |
| IL236447A0 (en) | 2015-02-26 |
| US20150147811A1 (en) | 2015-05-28 |
| KR102087258B1 (ko) | 2020-03-11 |
| CN104769102A (zh) | 2015-07-08 |
| MX2015000680A (es) | 2015-04-10 |
| WO2014013045A1 (de) | 2014-01-23 |
| US10081789B2 (en) | 2018-09-25 |
| ES2717278T3 (es) | 2019-06-20 |
| LT2875123T (lt) | 2019-04-10 |
| PT2875123T (pt) | 2019-04-22 |
| SI2875123T1 (sl) | 2019-05-31 |
| BR112015001120A2 (pt) | 2017-06-27 |
| CA2882100A1 (en) | 2014-01-23 |
| IN2014DN11263A (es) | 2015-10-09 |
| DK2875123T3 (en) | 2019-04-08 |
| HRP20190464T1 (hr) | 2019-05-03 |
| CN104769102B (zh) | 2020-11-03 |
| JP6693743B2 (ja) | 2020-05-13 |
| AU2013291990B2 (en) | 2019-01-03 |
| AU2013291990A1 (en) | 2015-01-29 |
| JP2015522287A (ja) | 2015-08-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |