JP6151879B2 - 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激 - Google Patents

体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激 Download PDF

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JP6151879B2
JP6151879B2 JP2010522965A JP2010522965A JP6151879B2 JP 6151879 B2 JP6151879 B2 JP 6151879B2 JP 2010522965 A JP2010522965 A JP 2010522965A JP 2010522965 A JP2010522965 A JP 2010522965A JP 6151879 B2 JP6151879 B2 JP 6151879B2
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cells
cell
wnt
reprogramming
wnt pathway
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JP2010537634A (ja
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ブレット チェバリアー，
ブレット チェバリアー，
アレキサンダー マーソン，
アレキサンダー マーソン，
リチャード エー． ヤング，
リチャード エー． ヤング，
ラス フォアマン，
ラス フォアマン，
ラドルフ ジェーニッシュ，
ラドルフ ジェーニッシュ，
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Whitehead Institute for Biomedical Research
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JP2010522965A 2007-08-31 2008-08-29 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激 Active JP6151879B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US96702807P 2007-08-31 2007-08-31
US60/967,028 2007-08-31
US18819008P 2008-08-06 2008-08-06
US61/188,190 2008-08-06
PCT/US2008/010249 WO2009032194A1 (en) 2007-08-31 2008-08-29 Wnt pathway stimulation in reprogramming somatic cells

Related Child Applications (2)

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JP2014020995A Division JP2014087373A (ja) 2007-08-31 2014-02-06 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2016129722A Division JP6470233B2 (ja) 2007-08-31 2016-06-30 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激

Publications (3)

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JP2010537634A JP2010537634A (ja) 2010-12-09
JP2010537634A5 JP2010537634A5 (enExample) 2011-10-13
JP6151879B2 true JP6151879B2 (ja) 2017-06-21

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JP2010522965A Active JP6151879B2 (ja) 2007-08-31 2008-08-29 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2014020995A Pending JP2014087373A (ja) 2007-08-31 2014-02-06 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2016129722A Active JP6470233B2 (ja) 2007-08-31 2016-06-30 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2017104383A Withdrawn JP2017140054A (ja) 2007-08-31 2017-05-26 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2019078537A Withdrawn JP2019115358A (ja) 2007-08-31 2019-04-17 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2022067614A Pending JP2022087314A (ja) 2007-08-31 2022-04-15 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2022067613A Active JP7377309B2 (ja) 2007-08-31 2022-04-15 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2024080055A Pending JP2024100852A (ja) 2007-08-31 2024-05-16 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激

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JP2014020995A Pending JP2014087373A (ja) 2007-08-31 2014-02-06 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2016129722A Active JP6470233B2 (ja) 2007-08-31 2016-06-30 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2017104383A Withdrawn JP2017140054A (ja) 2007-08-31 2017-05-26 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2019078537A Withdrawn JP2019115358A (ja) 2007-08-31 2019-04-17 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2022067614A Pending JP2022087314A (ja) 2007-08-31 2022-04-15 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2022067613A Active JP7377309B2 (ja) 2007-08-31 2022-04-15 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激
JP2024080055A Pending JP2024100852A (ja) 2007-08-31 2024-05-16 体細胞を再プログラミングすることにおけるｗｎｔ経路の刺激

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US (6) US9102919B2 (enExample)
EP (2) EP3078738B1 (enExample)
JP (8) JP6151879B2 (enExample)
CN (1) CN101855338B (enExample)
AU (5) AU2008297011B2 (enExample)
CA (2) CA2698091C (enExample)
ES (2) ES2589122T3 (enExample)
MX (1) MX2010002242A (enExample)
RU (1) RU2492232C2 (enExample)
WO (1) WO2009032194A1 (enExample)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
KR101420740B1 (ko) 2005-12-13 2014-07-17 교또 다이가꾸 핵초기화 인자
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
CN105861443A (zh) * 2007-04-07 2016-08-17 怀特黑德生物医学研究所 体细胞重编程
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
EP3078738B1 (en) 2007-08-31 2020-05-20 Whitehead Institute for Biomedical Research Wnt pathway stimulation in reprogramming somatic cells
EP2090649A1 (en) * 2008-02-13 2009-08-19 Fondazione Telethon Method for reprogramming differentiated cells
WO2009102983A2 (en) * 2008-02-15 2009-08-20 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
ES2690554T3 (es) * 2008-03-17 2018-11-21 The Scripps Research Institute Enfoques químicos y genéticos combinados para la generación de células madre pluripotentes inducidas
ES2722198T3 (es) 2008-05-02 2019-08-08 Univ Kyoto Método de reprogramación nuclear
EP3447128A1 (en) 2008-06-04 2019-02-27 FUJIFILM Cellular Dynamics, Inc. Methods for the production of ips cells using non-viral approach
CN105671065A (zh) * 2008-06-13 2016-06-15 怀特黑德生物医学研究所 细胞编程和重编程
CN102171348A (zh) 2008-08-12 2011-08-31 细胞动力国际有限公司 产生ips细胞的方法
EP2376626A4 (en) * 2008-12-13 2012-10-17 Dna Microarray MICRO-ENVIRONMENTAL NICHE ASSAY FOR SCREENING OF INDUCED PLURIPOTENT STEM CELLS (CIPS)
ES2645869T3 (es) 2008-12-17 2017-12-11 The Scripps Research Institute Generación y mantenimiento de células madre
ES2664590T3 (es) * 2009-05-18 2018-04-20 Curna, Inc. Tratamiento de enfermedades relacionadas con factores de reprogramación por inhibición del tránscrito antisentido natural a un factor de reprogramación
CN103087991B (zh) * 2009-06-05 2018-06-12 富士胶片细胞动力公司 重编程t细胞和造血细胞的方法
JP2011004674A (ja) * 2009-06-26 2011-01-13 Fujitsu Ltd 誘導多能性幹細胞（ｉＰＳ細胞）の製造方法
US9175265B2 (en) 2009-07-08 2015-11-03 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing extended self-renewal of functionally differentiated somatic cells
EP2451982B1 (en) 2009-07-09 2014-11-12 Whitehead Institute For Biomedical Research Compositions and methods for mammalian genetics and uses thereof
JP5751548B2 (ja) * 2009-08-07 2015-07-22 国立大学法人京都大学 イヌｉＰＳ細胞及びその製造方法
ES2638464T3 (es) 2009-10-16 2017-10-20 The Scripps Research Institute Inducción de células pluripotentes
US9453205B2 (en) 2009-10-31 2016-09-27 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
AU2010312240B2 (en) * 2009-10-31 2016-12-15 Genesis Technologies Limited Methods for reprogramming cells and uses thereof
US20140038291A1 (en) 2009-10-31 2014-02-06 New World Laboratories Inc. Methods for reprogramming cells and uses thereof
ES2539487T3 (es) * 2009-11-04 2015-07-01 Cellular Dynamics International, Inc. Reprogramación episómica con compuestos químicos
EP2499237A1 (en) * 2009-11-13 2012-09-19 INSERM (Institut National de la Santé et de la Recherche Médicale) Reprogrammation of eukaryotic cells with engineered microvesicles
AU2011235212B2 (en) 2010-03-31 2014-07-31 The Scripps Research Institute Reprogramming cells
EP3399026B1 (en) 2010-06-14 2024-06-26 The Scripps Research Institute Reprogramming of cells to a new fate
JPWO2011158852A1 (ja) * 2010-06-15 2013-08-19 国立大学法人 東京大学 誘導型多能性幹細胞の製造方法
CN106893692B (zh) 2010-12-22 2021-11-26 菲特治疗公司 用于单细胞分选与增强ipsc重新编程的细胞培养平台
ES2575605T3 (es) * 2010-12-31 2016-06-29 Universität Für Bodenkultur Wien Método de generación de células madre pluripotentes inducidas y células diferenciadas
CN107988381B (zh) 2011-07-25 2021-05-28 国立大学法人京都大学 筛选诱导的多能干细胞的方法
JP6208667B2 (ja) * 2011-10-03 2017-10-04 ユニベルシテ リブレ デ ブリュッセル 持続的ｈｉｖ感染症を処置するためのｈｉｖ−１遺伝子発現の再活性化
JP6162604B2 (ja) 2011-10-21 2017-07-12 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
RU2477314C1 (ru) * 2011-12-07 2013-03-10 Федеральное государственное бюджетное учреждение "Новосибирский научно-исследовательский институт патологии кровообращения имени академика Е.Н. Мешалкина" Минздравсоцразвития РФ, (ФГБУ "ННИИПК им. акад. Е.Н. Мешалкина" Минздравсоцразвития России) РЕКОМБИНАНТНАЯ ПЛАЗМИДА pSM-ZsGreen, КОДИРУЮЩАЯ БЕЛКИ SOX2 И С-MYC ЧЕЛОВЕКА И ФЛУОРЕСЦЕНТНЫЙ БЕЛОК ZsGreen, ПРЕДНАЗНАЧЕННАЯ ДЛЯ ПОЛУЧЕНИЯ ИНДУЦИРОВАННЫХ ПЛЮРИПОТЕНТНЫХ СТВОЛОВЫХ КЛЕТОК ЧЕЛОВЕКА
WO2013159103A1 (en) * 2012-04-20 2013-10-24 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
SG11201503167XA (en) * 2012-10-23 2015-05-28 Univ Kyoto Method of efficiently establishing induced pluripotent stem cells
JP6495658B2 (ja) 2013-02-08 2019-04-03 国立大学法人京都大学 巨核球及び血小板の製造方法
WO2014136581A1 (ja) 2013-03-06 2014-09-12 国立大学法人京都大学 多能性幹細胞の培養システム及び多能性幹細胞の継代方法
HK1220232A1 (zh) 2013-03-15 2017-04-28 怀特黑德生物医学研究院 用於神经变性疾病的细胞性发现平台
EP2977449B1 (en) 2013-03-21 2020-02-26 Kyoto University Pluripotent stem cell for neuronal differentiation induction
WO2014157257A1 (ja) 2013-03-25 2014-10-02 公益財団法人先端医療振興財団 細胞の選別方法
JP6461787B2 (ja) 2013-04-12 2019-01-30 国立大学法人京都大学 肺胞上皮前駆細胞の誘導方法
JP6429280B2 (ja) 2013-05-14 2018-11-28 国立大学法人京都大学 効率的な心筋細胞の誘導方法
CN105247041B (zh) 2013-05-31 2018-04-20 爱心细胞有限公司 组入有水凝胶的层叠细胞膜片
EP3020803B1 (en) 2013-06-11 2020-03-11 Kyoto University Method for producing renal precursor cells
US9796962B2 (en) 2013-08-07 2017-10-24 Kyoto University Method for generating pancreatic hormone-producing cells
SG11201601720RA (en) 2013-09-05 2016-04-28 Univ Kyoto New method for inducing dopamine-producing neural precursor cells
JP6635505B2 (ja) 2013-11-01 2020-01-29 国立大学法人京都大学 新規軟骨細胞誘導方法
SG10201807292YA (en) 2014-03-04 2018-09-27 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
US11066649B2 (en) 2014-03-19 2021-07-20 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing human cholangiocyte differentiation
EP3929302A1 (en) 2014-07-14 2021-12-29 Chugai Seiyaku Kabushiki Kaisha Method for identifying epitope on protein
US12241089B2 (en) 2014-07-18 2025-03-04 Kyoto University Method for inducing t cells for cell-based immunotherapy from pluripotent stem cells
US11261453B2 (en) 2014-09-12 2022-03-01 Whitehead Institute For Biomedical Research Cells expressing apolipoprotein E and uses thereof
WO2016104717A1 (ja) 2014-12-26 2016-06-30 国立大学法人京都大学 肝細胞誘導方法
JP2018504122A (ja) 2015-01-26 2018-02-15 フェイト セラピューティクス，インコーポレイテッド 免疫調節特性が増加した細胞ならびにその使用および製造のための方法
HK1248270A1 (zh) 2015-02-17 2018-10-12 大学健康网路 制备和使用窦房结样起搏器心肌细胞和心室样心肌细胞的方法
EP3081638A1 (en) 2015-04-16 2016-10-19 Kyoto University Method for producing pseudo-islets
RU2621547C2 (ru) * 2015-06-26 2017-06-06 Андрей Степанович БРЮХОВЕЦКИЙ Способ дистанционной мультиволновой электромагнитной радионейроинженерии головного мозга человека
BR112018001232A2 (pt) 2015-07-21 2018-09-25 The Children's Medical Center Corporation pd-l1 que expressa células-tronco hematopoéticas e usos
AU2016338680B2 (en) 2015-10-16 2022-11-17 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
PL3444334T3 (pl) 2016-04-15 2022-01-10 Kyoto University Sposób indukowania limfocytów t cd8-dodatnich swoistych wobec antygenu
US11898163B2 (en) 2016-04-22 2024-02-13 Kyoto University Method for producing dopaminergic neuron progenitor cell
WO2017196175A1 (en) 2016-05-12 2017-11-16 Erasmus University Medical Center Rotterdam A method for culturing myogenic cells, cultures obtained therefrom, screening methods, and cell culture medium.
WO2018064460A1 (en) * 2016-09-30 2018-04-05 Mayo Foundation For Medical Education And Research Viral vectors for nuclear reprogramming
WO2018124118A1 (ja) 2016-12-27 2018-07-05 住友化学株式会社 人工多能性幹細胞の評価方法及び選抜方法、並びに人工多能性幹細胞の製造方法
EP4053268B1 (en) 2017-01-20 2025-10-08 Kyoto University Method for producing cd8alpha+beta+cytotoxic t cells
US20210130785A1 (en) 2017-01-26 2021-05-06 Osaka University Medium for inducing differentiation of stem cells into mesodermal cells and method for producing mesodermal cells
JP7171055B2 (ja) 2017-03-14 2022-11-15 国立大学法人京都大学 多能性幹細胞からヘルパーｔ細胞を製造する方法
KR102761727B1 (ko) 2017-05-25 2025-02-03 고쿠리츠 다이가쿠 호진 교토 다이가쿠 중간 중배엽 세포로부터 신장 전구 세포로의 분화 유도 방법 및 다능성 줄기세포로부터 신장 전구 세포로의 분화 유도 방법
EP3643780A4 (en) 2017-06-19 2021-04-07 Foundation for Biomedical Research and Innovation at Kobe METHOD FOR PREDICTING THE DIFFERENTIATION CAPACITY OF PLURIPOTENT STEM CELLS, AND ASSOCIATED REAGENT
JP6758631B2 (ja) 2017-06-19 2020-09-23 国立大学法人大阪大学 角膜内皮細胞マーカー及びその利用
NL2019517B1 (en) 2017-09-08 2019-03-19 Univ Erasmus Med Ct Rotterdam New therapy for Pompe disease
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation TREATMENT OF TYPE 1 DIABETES AND AUTOIMMUNE DISEASES OR DISORDERS
EP3699267A4 (en) 2017-10-17 2021-10-27 Kyoto University PROCESS FOR OBTAINING AN ARTIFICIAL NEUROMUSCULAR JUNCTION FROM PLURIPOTENT STEM CELLS
BR112021000437A2 (pt) 2018-07-13 2021-04-06 Kyoto University Métodos para produzir uma célula t gama delta e para prevenir ou tratar tumor, célula t gama delta, população de célula, medicamento, agente de extermínio para uma célula, e, uso da célula
EP3824912A4 (en) 2018-07-19 2022-04-20 Kyoto University PLATEFORM CARTILAGE DERIVED FROM PLURIPOTENTS STEM CELLS AND METHODS FOR PRODUCTION OF PLATEFORM CARTILAGE
US20210332329A1 (en) 2018-07-23 2021-10-28 Kyoto University Novel renal progenitor cell marker and method for concentrating renal progenitor cells using same
US12168781B2 (en) 2018-12-06 2024-12-17 Kirin Holdings Kabushiki Kaisha Production method for T cells or NK cells, medium for culturing T cells or NK cells, method for culturing T cells or NK cells, method for maintaining undifferentiated state of undifferentiated T cells, and growth-accelerating agent for T cells or NK cells
EP3900787A4 (en) 2018-12-21 2022-02-23 Kyoto University LUBRICIN-LOCALIZED CARTILE-LIKE TISSUE, METHOD OF MANUFACTURE THEREOF AND COMPOSITION THEREOF FOR TREATMENT OF ARTICULATE-CARTILAGE DEFECTS
CN113226475A (zh) 2018-12-26 2021-08-06 麒麟控股株式会社 改造tcr及其制造方法
EP3969571A4 (en) 2019-05-14 2023-05-24 Aleph Farms Ltd. AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE
EP3974519A4 (en) 2019-05-20 2023-07-12 Ajinomoto Co., Inc. EXPANSION CULTURE METHOD FOR CARTILAGE OR BONE PRECURSOR CELLS
US20220403337A1 (en) * 2019-11-14 2022-12-22 Takeo SHIMASAKI Cell reprogramming method
EP4074321A4 (en) 2019-12-12 2024-01-03 National University Corporation Chiba University Freeze-dried preparation containing megakaryocytes and platelets
AU2021225952A1 (en) 2020-02-28 2022-10-20 Takeda Pharmaceutical Company Limited Method for producing natural killer cells from pluripotent stem cells
EP3922431A1 (en) 2020-06-08 2021-12-15 Erasmus University Medical Center Rotterdam Method of manufacturing microdevices for lab-on-chip applications
JPWO2021256522A1 (enExample) 2020-06-17 2021-12-23
EP4180516A4 (en) 2020-07-13 2024-01-17 Kyoto University SKELETON MUSCLE PRECURSOR CELLS AND METHOD FOR PURIFICATION THEREOF, COMPOSITION FOR THE TREATMENT OF MYOGENIC DISEASES AND METHOD FOR PRODUCING SKELETON MUSCLE PRECURSOR CELLS CONTAINING CELL GROUPS
JPWO2022019152A1 (enExample) 2020-07-20 2022-01-27
US20240271088A1 (en) 2020-08-18 2024-08-15 Kyoto University Method for maintaining and amplifying human primordial germ cells / human primordial germ cell-like cells
WO2022115492A1 (en) 2020-11-24 2022-06-02 Lyell Immunopharma, Inc. Methods for making, compositions comprising, and methods of using rejuvenated t cells
US20240182858A1 (en) 2021-03-17 2024-06-06 Astellas Pharma Inc. Pericyte having basic fibroblast growth factor (bfgf) gene introduced therein
WO2022230977A1 (ja) 2021-04-30 2022-11-03 国立研究開発法人理化学研究所 網膜色素上皮細胞のひも状凝集体、それを製造するためのデバイスおよび製造方法、ならびに該ひも状凝集体を含有する治療薬
US20240254441A1 (en) 2021-06-04 2024-08-01 Kirin Holdings Kabushiki Kaisha Cell composition, method for producing cell composition, and pharmaceutical composition containing cell composition
WO2022259721A1 (ja) 2021-06-10 2022-12-15 味の素株式会社 間葉系幹細胞の製造方法
CN117813374A (zh) 2021-06-15 2024-04-02 武田药品工业株式会社 从多能干细胞产生自然杀伤细胞的方法
WO2023286834A1 (ja) 2021-07-15 2023-01-19 アステラス製薬株式会社 血管内皮増殖因子（ｖｅｇｆ）高発現ペリサイト様細胞
JPWO2023286832A1 (enExample) 2021-07-15 2023-01-19
JPWO2023017848A1 (enExample) 2021-08-11 2023-02-16
US20250041418A1 (en) 2021-11-11 2025-02-06 Healios K.K. Gene-modified pluripotent stem cell, immunocompetent cell derived therefrom, method for producing said cells, and use thereof
JPWO2023153464A1 (enExample) 2022-02-09 2023-08-17
US20250290076A1 (en) 2022-04-27 2025-09-18 Kyoto University Epicardial cell regeneration promoter and method for promoting epicardial cell regeneration
US20250376727A1 (en) 2022-06-10 2025-12-11 Kyoto University Detection method and detection reagent for undifferentiated pluripotent stem cells

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010218A1 (en) 1991-11-14 1993-05-27 The United States Government As Represented By The Secretary Of The Department Of Health And Human Services Vectors including foreign genes and negative selective markers
US5698446A (en) 1994-09-07 1997-12-16 Chiron Corporation Methods and compositions for inhibiting production of replication competent virus
US5712171A (en) 1995-01-20 1998-01-27 Arqule, Inc. Method of generating a plurality of chemical compounds in a spatially arranged array
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6057117A (en) 1996-04-04 2000-05-02 Chiron Corporation Identification and use of selective inhibitors of glycogen synthase kinase 3
US20010012513A1 (en) 1996-08-19 2001-08-09 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
DE69916302T2 (de) 1998-11-11 2005-05-04 Emory University Verwendung von dendritischen zellen-depletierten zusammensetzungen zur verbesserung der transplantation von allogenen hämatopoietischen zellen
IL129966A (en) 1999-05-14 2009-12-24 Technion Res & Dev Foundation ISOLATED HUMAN EMBRYOID BODIES (hEB) DERIVED FROM HUMAN EMBRYONIC STEM CELLS
ATE253918T1 (de) 1999-12-08 2003-11-15 Centre Nat Rech Scient Verwendung von hymenialdisin und dessen derivaten zur herstellung therapeutischer mittel
ATE384704T1 (de) 1999-12-17 2008-02-15 Novartis Vaccines & Diagnostic Pyrazin-basierte hemmer der glycogen-synthase- kinase 3
FR2804959B1 (fr) 2000-02-15 2006-04-28 Centre Nat Rech Scient Utilisation de derives de paullones pour la fabrication de medicaments
US20040087016A1 (en) * 2000-05-12 2004-05-06 University Of Utah Research Foundation Compositions and methods for cell dedifferentiation and tissue regeneration
US20030096813A1 (en) 2001-04-20 2003-05-22 Jingrong Cao Compositions useful as inhibitors of GSK-3
EP1406561A4 (en) * 2001-07-16 2008-03-12 Edwards Lifesciences Corp FABRIC MADE OF FABRIC
US6916798B2 (en) 2001-08-03 2005-07-12 Vertex Pharmaceuticals Incorporated Inhibitors of GSK-3 and uses thereof
MXPA04005510A (es) 2001-12-07 2006-02-24 Vertex Pharma Compuestos basados en pirimidina utiles como inhibidores de gsk-3.
CA2476214A1 (en) * 2002-02-08 2003-08-14 University Of South Florida Proliferated cell lines and uses thereof
US6835810B2 (en) 2002-05-13 2004-12-28 Geneshuttle Biopharma, Inc. Fusion protein for use as vector
BR0313743A (pt) 2002-08-23 2005-07-05 Chiron Corp Benzimidazol quinolinonas e usos destas
US7119200B2 (en) 2002-09-04 2006-10-10 Schering Corporation Pyrazolopyrimidines as cyclin dependent kinase inhibitors
US7153832B2 (en) 2003-04-07 2006-12-26 The Board Of Trustees Of The Leland Stanford Junior University Compositions of active Wnt protein
KR20060056377A (ko) 2003-08-13 2006-05-24 카이론 코포레이션 Ｇｓｋ-3 억제제 및 그것의 사용
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US20060030042A1 (en) * 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US7964401B2 (en) * 2004-02-19 2011-06-21 Kyoto University Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3
EP1574499A1 (en) 2004-03-08 2005-09-14 DKFZ Deutsches Krebsforschungszentrum Inhibitors of DNA methylation in tumor cells
US7850960B2 (en) 2004-12-30 2010-12-14 University Of Washington Methods for regulation of stem cells
WO2006091737A1 (en) 2005-02-24 2006-08-31 Kemia, Inc. Modulators of gsk-3 activity
WO2007007054A1 (en) 2005-07-08 2007-01-18 Cancer Research Technology Limited Phthalamides, succinimides and related compounds and their use as pharmaceuticals
US20070128669A1 (en) 2005-11-28 2007-06-07 Institute For Chemical Genomics Serum-free expansion of cells in culture
KR101420740B1 (ko) 2005-12-13 2014-07-17 교또 다이가꾸 핵초기화 인자
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US9074180B2 (en) 2006-03-30 2015-07-07 The University Court Of The University Of Edinburgh Culture medium containing kinase inhibitors, and uses thereof
GB0615327D0 (en) 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
CN105861443A (zh) 2007-04-07 2016-08-17 怀特黑德生物医学研究所 体细胞重编程
EP3078738B1 (en) * 2007-08-31 2020-05-20 Whitehead Institute for Biomedical Research Wnt pathway stimulation in reprogramming somatic cells
EP2250252A2 (en) 2008-02-11 2010-11-17 Cambridge Enterprise Limited Improved reprogramming of mammalian cells, and the cells obtained
CN105671065A (zh) 2008-06-13 2016-06-15 怀特黑德生物医学研究所 细胞编程和重编程
WO2010033920A2 (en) 2008-09-19 2010-03-25 Whitehead Institute For Biomedical Research Compositions and methods for enhancing cell reprogramming
US20110088107A1 (en) 2009-04-24 2011-04-14 Yaqub Hanna Compositions and methods for deriving or culturing pluripotent cells

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