ES2722198T3 - Método de reprogramación nuclear - Google Patents
Método de reprogramación nuclear Download PDFInfo
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- ES2722198T3 ES2722198T3 ES09738908T ES09738908T ES2722198T3 ES 2722198 T3 ES2722198 T3 ES 2722198T3 ES 09738908 T ES09738908 T ES 09738908T ES 09738908 T ES09738908 T ES 09738908T ES 2722198 T3 ES2722198 T3 ES 2722198T3
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/02—Details
- H01J37/24—Circuit arrangements not adapted to a particular application of the tube and not otherwise provided for
- H01J37/241—High voltage power supply or regulation circuits
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- H01J37/00—Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
- H01J37/32—Gas-filled discharge tubes
- H01J37/32009—Arrangements for generation of plasma specially adapted for examination or treatment of objects, e.g. plasma sources
- H01J37/32073—Corona discharge
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1361—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from dental pulp or dental follicle stem cells
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- C12N2510/00—Genetically modified cells
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- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
- H01J2237/03—Mounting, supporting, spacing or insulating electrodes
- H01J2237/038—Insulating
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J2237/00—Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
- H01J2237/06—Sources
- H01J2237/063—Electron sources
- H01J2237/06375—Arrangement of electrodes
Abstract
Un método para producir una célula madre pluripotencial inducida, que comprende la etapa de introducir dos o más tipos de vectores de expresión no víricos diferentes para introducir cuatro o más tipos de genes que codifican un factor de reprogramación en una célula somática, en el que los vectores de expresión no víricos son vectores plasmídicos de replicación autónoma fuera de un cromosoma, en el que los genes que codifican un factor de reprogramación se seleccionan del grupo que consiste en un gen de la familia Oct, un gen de la familia Klf, un gen de la familia Sox, un gen de la familia Myc, un gen de la familia Lin y el gen Nanog, y en el que todos los tipos de vectores de expresión no víricos se introducen al mismo tiempo en la célula somática.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7150808P | 2008-05-02 | 2008-05-02 | |
US13624608P | 2008-08-21 | 2008-08-21 | |
US13661508P | 2008-09-19 | 2008-09-19 | |
US19336308P | 2008-11-21 | 2008-11-21 | |
PCT/JP2009/058873 WO2009133971A1 (en) | 2008-05-02 | 2009-05-01 | Method of nuclear reprogramming |
Publications (1)
Publication Number | Publication Date |
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ES2722198T3 true ES2722198T3 (es) | 2019-08-08 |
Family
ID=41255176
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09738908T Active ES2722198T3 (es) | 2008-05-02 | 2009-05-01 | Método de reprogramación nuclear |
Country Status (9)
Country | Link |
---|---|
US (5) | US20100279404A1 (es) |
EP (1) | EP2268809B1 (es) |
JP (1) | JP5346925B2 (es) |
KR (1) | KR101661940B1 (es) |
CN (1) | CN101855350B (es) |
CA (1) | CA2695522C (es) |
ES (1) | ES2722198T3 (es) |
SG (1) | SG10201400329YA (es) |
WO (1) | WO2009133971A1 (es) |
Families Citing this family (46)
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US7682828B2 (en) | 2003-11-26 | 2010-03-23 | Whitehead Institute For Biomedical Research | Methods for reprogramming somatic cells |
ES2836764T3 (es) | 2005-08-03 | 2021-06-28 | Astellas Inst For Regenerative Medicine | Métodos mejorados de reprogramación de células somáticas animales |
US8129187B2 (en) | 2005-12-13 | 2012-03-06 | Kyoto University | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
EP4223769A3 (en) | 2005-12-13 | 2023-11-01 | Kyoto University | Nuclear reprogramming factor |
US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
CN101743306A (zh) | 2007-03-23 | 2010-06-16 | 威斯康星校友研究基金会 | 体细胞重编程 |
MX348010B (es) | 2007-04-07 | 2017-05-23 | Whitehead Inst Biomedical Res | Reprogramacion de celulas somaticas. |
US9213999B2 (en) | 2007-06-15 | 2015-12-15 | Kyoto University | Providing iPSCs to a customer |
JP2008307007A (ja) | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
EP2268809B1 (en) | 2008-05-02 | 2019-02-06 | Kyoto University | Method of nuclear reprogramming |
ES2587395T3 (es) | 2008-06-04 | 2016-10-24 | Cellular Dynamics International, Inc. | Procedimientos para la producción de células IPS usando un enfoque no vírico |
AU2015202237B2 (en) * | 2008-06-13 | 2017-09-28 | Whitehead Institute For Biomedical Research | Programming and reprogramming of cells |
ES2644588T3 (es) * | 2008-06-13 | 2017-11-29 | Whitehead Institute For Biomedical Research | Programación y reprogramación de células |
US9127256B2 (en) * | 2008-07-16 | 2015-09-08 | Dnavec Corporation | Method for production of reprogrammed cell using chromosomally unintegrated virus vector |
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WO2010042490A1 (en) * | 2008-10-06 | 2010-04-15 | Boston Medical Center Corporation | A single lentiviral vector system for induced pluripotent (ips) stem cells derivation |
CN102239249A (zh) * | 2008-10-24 | 2011-11-09 | 威斯康星校友研究基金会 | 通过非病毒重编程获得的多潜能干细胞 |
CA2755870C (en) * | 2009-03-20 | 2019-04-09 | Angioblast Systems, Inc. | Production of reprogrammed pluripotent cells |
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WO2011111614A1 (en) * | 2010-03-10 | 2011-09-15 | Kyoto University | Method of selecting induced pluripotent stem cell |
EP3936608A1 (en) * | 2010-03-31 | 2022-01-12 | The Scripps Research Institute | Reprogramming cells |
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JP5908838B2 (ja) * | 2010-08-30 | 2016-04-26 | 株式会社Idファーマ | 多能性幹細胞を誘導するための組成物およびその使用 |
EP2627342B1 (en) * | 2010-10-14 | 2016-08-24 | University Of Central Florida Research Foundation, Inc. | Cardiac induced pluripotent stem cells and methods of use in repair and regeneration of myocardium |
EP2647699B1 (en) * | 2010-12-03 | 2020-04-01 | Kyoto University | Efficient method for establishing induced pluripotent stem cells |
WO2012098260A1 (en) | 2011-01-21 | 2012-07-26 | Axiogenesis Ag | A non-viral system for the generation of induced pluripotent stem (ips) cells |
US9228204B2 (en) | 2011-02-14 | 2016-01-05 | University Of Utah Research Foundation | Constructs for making induced pluripotent stem cells |
US9932560B2 (en) | 2011-05-13 | 2018-04-03 | Elixirgen, Llc | Use of Zscan4 and Zscan4-dependent genes for direct reprogramming of somatic cells |
US10865383B2 (en) | 2011-07-12 | 2020-12-15 | Lineage Cell Therapeutics, Inc. | Methods and formulations for orthopedic cell therapy |
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JP6617231B2 (ja) * | 2013-08-28 | 2019-12-11 | 国立大学法人岐阜大学 | 人工多能性幹細胞の作製方法 |
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EP3359169A4 (en) | 2015-10-05 | 2019-03-13 | Orig3N, Inc. | DIAGNOSIS AND TREATMENT OF MORBUS PARKINSON BASED ON THE IDENTIFICATION AND REDUCTION OF LIVER FUNCTIONAL DISORDERS |
CA3025057A1 (en) | 2016-05-25 | 2017-11-30 | Inserm (Institut National De La Sante Et De La Recherche Medicale) | Methods and compositions for treating cancers |
US11572545B2 (en) | 2016-06-16 | 2023-02-07 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
US10221395B2 (en) | 2016-06-16 | 2019-03-05 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
PL3644728T3 (pl) * | 2017-06-28 | 2022-12-05 | Sci-Group As | Zamrażanie materiału biologicznego |
US11679148B2 (en) | 2017-11-24 | 2023-06-20 | Institut National De La Santé Et De La Recherche Médicale (Inserm) | Methods and compositions for treating cancers |
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- 2009-05-01 JP JP2010506477A patent/JP5346925B2/ja active Active
- 2009-05-01 WO PCT/JP2009/058873 patent/WO2009133971A1/en active Application Filing
- 2009-05-01 KR KR1020107002311A patent/KR101661940B1/ko active IP Right Grant
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US20200172875A1 (en) | 2020-06-04 |
US20130065311A1 (en) | 2013-03-14 |
US9499797B2 (en) | 2016-11-22 |
WO2009133971A1 (en) | 2009-11-05 |
CN101855350B (zh) | 2014-12-31 |
CA2695522A1 (en) | 2009-11-05 |
EP2268809B1 (en) | 2019-02-06 |
US20100279404A1 (en) | 2010-11-04 |
CA2695522C (en) | 2019-01-15 |
US20230282445A1 (en) | 2023-09-07 |
EP2268809A4 (en) | 2013-02-27 |
KR101661940B1 (ko) | 2016-10-04 |
JP2011519546A (ja) | 2011-07-14 |
SG10201400329YA (en) | 2014-05-29 |
KR20110015500A (ko) | 2011-02-16 |
CN101855350A (zh) | 2010-10-06 |
EP2268809A1 (en) | 2011-01-05 |
US20150072417A1 (en) | 2015-03-12 |
JP5346925B2 (ja) | 2013-11-20 |
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