SG10201400329YA - Method of nuclear reprogramming - Google Patents

Method of nuclear reprogramming

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Publication number
SG10201400329YA
SG10201400329YA SG10201400329YA SG10201400329YA SG10201400329YA SG 10201400329Y A SG10201400329Y A SG 10201400329YA SG 10201400329Y A SG10201400329Y A SG 10201400329YA SG 10201400329Y A SG10201400329Y A SG 10201400329YA SG 10201400329Y A SG10201400329Y A SG 10201400329YA
Authority
SG
Singapore
Prior art keywords
nuclear reprogramming
reprogramming
nuclear
Prior art date
Application number
SG10201400329YA
Inventor
Shinya Yamanaka
Keisuke Okita
Original Assignee
Univ Kyoto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kyoto filed Critical Univ Kyoto
Publication of SG10201400329YA publication Critical patent/SG10201400329YA/en

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SG10201400329YA 2008-05-02 2009-05-01 Method of nuclear reprogramming SG10201400329YA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US7150808P 2008-05-02 2008-05-02
US13624608P 2008-08-21 2008-08-21
US13661508P 2008-09-19 2008-09-19
US19336308P 2008-11-21 2008-11-21

Publications (1)

Publication Number Publication Date
SG10201400329YA true SG10201400329YA (en) 2014-05-29

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ID=41255176

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Application Number Title Priority Date Filing Date
SG10201400329YA SG10201400329YA (en) 2008-05-02 2009-05-01 Method of nuclear reprogramming

Country Status (9)

Country Link
US (5) US20100279404A1 (en)
EP (1) EP2268809B1 (en)
JP (1) JP5346925B2 (en)
KR (1) KR101661940B1 (en)
CN (1) CN101855350B (en)
CA (1) CA2695522C (en)
ES (1) ES2722198T3 (en)
SG (1) SG10201400329YA (en)
WO (1) WO2009133971A1 (en)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
CA2659945C (en) * 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
BRPI0619794B8 (en) 2005-12-13 2022-06-14 Univ Kyoto Use of a reprogramming factor, agent for preparing an induced pluripotent stem cell from a somatic cell and methods for preparing an induced pluripotent stem cell method and for preparing a somatic cell and use of induced pluripotent stem cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
SG193653A1 (en) 2007-03-23 2013-10-30 Wisconsin Alumni Res Found Somatic cell reprogramming
WO2008124133A1 (en) 2007-04-07 2008-10-16 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
ES2722198T3 (en) 2008-05-02 2019-08-08 Univ Kyoto Nuclear reprogramming method
KR101871192B1 (en) 2008-06-04 2018-06-27 후지필름 셀룰러 다이내믹스, 인코포레이티드 Methods for the production of iPS cells using non-viral approach
AU2015202237B2 (en) * 2008-06-13 2017-09-28 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
AU2009257219B2 (en) 2008-06-13 2015-01-29 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
KR20110046472A (en) 2008-07-16 2011-05-04 디나벡크 가부시키가이샤 How to prepare reprogrammed cells using chromosomal non-inserted viral vectors
WO2010013845A1 (en) * 2008-07-30 2010-02-04 Kyoto University Method of efficiently establishing induced pluripotent stem cells
CN107988261A (en) * 2008-08-12 2018-05-04 细胞动力国际有限公司 The method for producing IPS cells
JP5557288B2 (en) * 2008-09-12 2014-07-23 株式会社chromocenter Multiple gene expression control system used for cell reprogramming
WO2010042490A1 (en) * 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
SG10201600234PA (en) 2008-10-24 2016-02-26 Wisconsin Alumni Res Found Pluripotent Stem Cells Obtained By Non-Viral Reprogramming
KR101764437B1 (en) 2009-03-20 2017-08-02 메소블라스트, 아이엔씨. Production of reprogrammed pluripotent cells
KR101811235B1 (en) 2009-06-05 2017-12-21 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 Reprogramming T cells and hematopoietic cells
EP2545163A4 (en) * 2010-03-10 2013-11-06 Univ Kyoto Method of selecting induced pluripotent stem cell
WO2011123572A1 (en) * 2010-03-31 2011-10-06 The Scripps Research Institute Reprogramming cells
EP3072961A1 (en) 2010-04-16 2016-09-28 Children's Medical Center Corporation Sustained polypeptide expression from synthetic, modified rnas and uses thereof
US8048675B1 (en) 2010-05-12 2011-11-01 Ipierian, Inc. Integration-free human induced pluripotent stem cells from blood
KR20130093624A (en) * 2010-08-30 2013-08-22 디나벡크 가부시키가이샤 Composition for inducing pluripotent stem cell, and use thereof
WO2012051515A2 (en) * 2010-10-14 2012-04-19 University Of Central Florida Research Foundation, Inc. Cardiac induced pluripotent stem cells and methods of use in repair and regeneration of myocardium
WO2012074117A1 (en) 2010-12-03 2012-06-07 国立大学法人京都大学 Efficient method for establishing artificial pluripotent stem cells
WO2012098260A1 (en) 2011-01-21 2012-07-26 Axiogenesis Ag A non-viral system for the generation of induced pluripotent stem (ips) cells
US9228204B2 (en) 2011-02-14 2016-01-05 University Of Utah Research Foundation Constructs for making induced pluripotent stem cells
WO2012158561A1 (en) 2011-05-13 2012-11-22 The United States Of America As Represented By The Secretary, Dept. Of Health And Human Services Use of zscan4 and zscan4-dependent genes for direct reprogramming of somatic cells
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
US20160115455A1 (en) * 2013-05-17 2016-04-28 The Broad Institute, Inc. Reprogrammed cells and methods of production and use thereof
WO2015006725A2 (en) * 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
WO2015030111A1 (en) 2013-08-28 2015-03-05 国立大学法人岐阜大学 Method for producing induced pluripotent stem cells
US20160304840A1 (en) 2013-11-01 2016-10-20 New England Biolabs, Inc. Method for Producing Induced Pluripotent Stem Cells
KR20180055901A (en) 2015-10-05 2018-05-25 오리그3엔, 인코포레이티드 Diagnosis and treatment of Parkinson's disease based on identification and improvement of liver dysfunction
KR102677451B1 (en) 2016-05-25 2024-06-24 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) Methods and compositions for treating cancers
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
PL3644728T3 (en) * 2017-06-28 2022-12-05 Sci-Group As Freezing of biological material
KR20200111168A (en) 2017-11-24 2020-09-28 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) Methods and compositions for cancer treatment
EP3833383A1 (en) 2018-08-06 2021-06-16 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for treating cancers
EP4314246A1 (en) 2021-04-01 2024-02-07 INSERM (Institut National de la Santé et de la Recherche Médicale) Liver organoid manufacturing methods, liver organoids obtained with the same, and uses thereof
CN113462638B (en) * 2021-06-30 2022-10-25 呈诺再生医学科技(珠海横琴新区)有限公司 Efficient genetic-modification-free iPSC induction and industrialization monoclonal picking platform and application
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion

Family Cites Families (160)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4650764A (en) * 1983-04-12 1987-03-17 Wisconsin Alumni Research Foundation Helper cell
US4861719A (en) * 1986-04-25 1989-08-29 Fred Hutchinson Cancer Research Center DNA constructs for retrovirus packaging cell lines
US4937190A (en) * 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5192553A (en) * 1987-11-12 1993-03-09 Biocyte Corporation Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use
US6140111A (en) * 1987-12-11 2000-10-31 Whitehead Institute For Biomedical Research Retroviral gene therapy vectors and therapeutic methods based thereon
US7070994B2 (en) * 1988-03-21 2006-07-04 Oxford Biomedica (Uk) Ltd. Packaging cells
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
JP2886547B2 (en) * 1988-07-26 1999-04-26 協和醗酵工業株式会社 Method for producing neuraminidase
JP3082204B2 (en) * 1988-09-01 2000-08-28 ホワイトヘッド・インスティチュート・フォー・バイオメディカル・リサーチ Recombinant retrovirus with an amphotropic and ecotropic host range
JP2928287B2 (en) 1988-09-29 1999-08-03 協和醗酵工業株式会社 Novel polypeptide
JP3051411B2 (en) * 1989-03-14 2000-06-12 持田製薬株式会社 Novel DNA and expression plasmid containing it
US5266491A (en) * 1989-03-14 1993-11-30 Mochida Pharmaceutical Co., Ltd. DNA fragment and expression plasmid containing the DNA fragment
JP2897295B2 (en) * 1989-12-14 1999-05-31 味の素株式会社 DNA construct for high production of retrovirus and cell line for high production of retrovirus
US5747641A (en) * 1989-12-21 1998-05-05 Biogen Inc Tat-derived transport polypeptide conjugates
US5817491A (en) * 1990-09-21 1998-10-06 The Regents Of The University Of California VSV G pseusdotyped retroviral vectors
US5288514A (en) * 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5834256A (en) * 1993-06-11 1998-11-10 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
FR2707091B1 (en) * 1993-06-30 1997-04-04 Cohen Haguenauer Odile Retroviral vector for gene transfer and expression in eukaryotic cells.
US5534423A (en) 1993-10-08 1996-07-09 Regents Of The University Of Michigan Methods of increasing rates of infection by directing motion of vectors
US5519134A (en) * 1994-01-11 1996-05-21 Isis Pharmaceuticals, Inc. Pyrrolidine-containing monomers and oligomers
US6013517A (en) * 1994-05-09 2000-01-11 Chiron Corporation Crossless retroviral vectors
US5525735A (en) * 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) * 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
JP4216350B2 (en) * 1994-09-19 2009-01-28 大日本住友製薬株式会社 Recombinant DNA viral vector for animal cell infection
DE69534166T2 (en) * 1994-10-28 2006-03-09 Trustees Of The University Of Pennsylvania RECOMBINANT ADENOVIRUS AND METHODS OF USE THEREOF
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5637456A (en) * 1995-02-17 1997-06-10 The University Of Texas, Board Of Regents Rapid test for determining the amount of functionally inactive gene in a gene therapy vector preparation
US5707618A (en) * 1995-03-24 1998-01-13 Genzyme Corporation Adenovirus vectors for gene therapy
US5830725A (en) * 1995-04-28 1998-11-03 The Board Of Trustees For The Leland Stanford Junior University Rapid, stable high-titre production of recombing retrovirus
US5744320A (en) * 1995-06-07 1998-04-28 Promega Corporation Quenching reagents and assays for enzyme-mediated luminescence
ATE365808T1 (en) 1995-07-28 2007-07-15 Marie Curie Cancer Care TRANSPORT PROTEINS AND THEIR USES
DE69611102T2 (en) * 1995-09-22 2001-06-21 Amersham Pharmacia Biotech Uk Ltd., Little Chalfont STRENGTHENING THE MUTAGENESIS OF NUCLEIC ACIDS
US5910434A (en) * 1995-12-15 1999-06-08 Systemix, Inc. Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant
FR2751345B1 (en) * 1996-07-16 1998-09-18 Univ Paris Curie HIGHLY PRODUCING PACKAGING LINES
US6255071B1 (en) * 1996-09-20 2001-07-03 Cold Spring Harbor Laboratory Mammalian viral vectors and their uses
US6025192A (en) * 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US6017735A (en) * 1997-01-23 2000-01-25 Marie Curie Cancer Care Materials and methods for intracellular transport and their uses
US6416959B1 (en) * 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
CZ302262B6 (en) * 1997-04-16 2011-01-19 Amgen Inc. Isolated nucleic acid, polypeptide encoded by the nucleic acid, expression vector comprising the nucleic acid, host cell transformed or transfected with the expression vector, isolated osteoprotegerin binding protein, antibody, which specifically bin
AU9200398A (en) * 1997-08-22 1999-03-16 Yale University A process to study changes in gene expression in granulocytic cells
WO2000018885A1 (en) 1998-09-29 2000-04-06 Gamida Cell Ltd. Methods of controlling proliferation and differentiation of stem and progenitor cells
US6835567B1 (en) 1998-04-14 2004-12-28 Signal Pharmaceuticals, Inc. PNS cell lines and methods of use therefor
US20020174013A1 (en) * 1998-04-17 2002-11-21 Viztec Inc., A Florida Corporation Chip card advertising method and system
GB9809178D0 (en) 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
US6146874A (en) 1998-05-27 2000-11-14 University Of Florida Method of preparing recombinant adeno-associated virus compositions
FR2779445B1 (en) 1998-06-08 2000-08-11 Univ Nantes ENCAPSIDATION KIT
KR20000006334A (en) * 1998-06-26 2000-01-25 이선경 High efficiency retroviral vectors that contain none of viral coding sequences
US6485959B1 (en) * 1998-10-07 2002-11-26 Cedars Sinai Medical Center Cell preconditioning and cryopresevation medium
EP1129175A2 (en) 1998-10-16 2001-09-05 Novartis AG Promotion of self-renewal and improved gene transduction of hematopoietic stem cells by histone deacetylase inhibitors
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
IL142748A0 (en) * 1998-11-09 2002-03-10 Univ Monash Embryonic stem cells
US6376246B1 (en) * 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6153432A (en) * 1999-01-29 2000-11-28 Zen-Bio, Inc Methods for the differentiation of human preadipocytes into adipocytes
US6312949B1 (en) * 1999-03-26 2001-11-06 The Salk Institute For Biological Studies Regulation of tyrosine hydroxylase expression
US6773920B1 (en) * 1999-03-31 2004-08-10 Invitrogen Corporation Delivery of functional protein sequences by translocating polypeptides
JP3904451B2 (en) * 1999-06-01 2007-04-11 俊雄 北村 Packaging cells
US7015037B1 (en) * 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
WO2001015511A2 (en) * 1999-09-01 2001-03-08 University Of Pittsburgh Of The Commonwealth System Of Higher Education Identification of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, dna and viruses
EP1218489B1 (en) 1999-09-24 2009-03-18 Cybios LLC Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
US20030161817A1 (en) * 2001-03-28 2003-08-28 Young Henry E. Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
US7544509B2 (en) * 2000-01-24 2009-06-09 Mcgill University Method for preparing stem cell preparations
US6395546B1 (en) * 2000-02-01 2002-05-28 Neurogeneration, Inc. Generation of dopaminergic neurons from human nervous system stem cells
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
WO2001088104A2 (en) 2000-05-17 2001-11-22 Geron Corporation Neural progenitor cell populations
WO2001096532A2 (en) * 2000-06-15 2001-12-20 Tanja Dominko Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast
DE10031179A1 (en) 2000-06-27 2002-01-31 Amaxa Gmbh Introducing active molecules into the nucleus of eukaryotic cells, useful for transfection and gene therapy, by applying controlled electrical pulse
EP1305333A4 (en) * 2000-07-31 2006-04-12 Active Motif Peptide-mediated delivery of molecules into cells
JP2002065261A (en) 2000-08-30 2002-03-05 Mitsubishi Kasei Institute Of Life Sciences Method for obtaining reproductive cell
EP1313642B1 (en) * 2000-08-31 2006-04-26 Edwin Lundgren Control device for a steering kite on a boat
JP2004520046A (en) 2000-11-27 2004-07-08 イスム リサーチ ディベロップメント カンパニー オブ ザ ヘブライ ユニバーシティ オブ エルサレム Transfection of human embryonic stem cells
US20080268054A1 (en) 2000-12-04 2008-10-30 Eugene Bell Dermal derived human stem cells and compositions and methods thereof
CA2433419A1 (en) * 2001-01-02 2002-07-25 Stemron, Inc. A method for producing a population of homozygous stem cells having a pre-selected immunotype and/or genotype, cells suitable for transplant derived therefrom, and materials and methods using same
JP2003009854A (en) 2001-04-09 2003-01-14 Kyowa Hakko Kogyo Co Ltd Method for embryoid body formation and use thereof
EP1390518B1 (en) 2001-04-23 2004-10-06 Amaxa GmbH Buffer solution for electroporation and a method comprising the use of the same
DE10119901A1 (en) 2001-04-23 2002-10-24 Amaxa Gmbh Apparatus for electrophoretic transfer of biologically active molecules into cells comprises capacitors connected to high voltage sources, which discharge via power transistor into cuvette holding sample
ES2544854T3 (en) * 2001-05-31 2015-09-04 Shinya Yamanaka Genes with specific ES cell expression
WO2003018780A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
CA2461121A1 (en) * 2001-09-20 2003-04-03 Kyowa Hakko Kogyo Co., Ltd. Multipotent stem cell in the interstitial tissues of skeletal muscle
JP2004248505A (en) 2001-09-21 2004-09-09 Norio Nakatsuji Undifferentiated fusion cell of somatic cell derived from es cell deficient in part or all of transplantation antigen and method for producing the same
CA2461185A1 (en) 2001-09-21 2003-04-03 Japan Science And Technology Corporation Method of screening reprogramming factor, reprogramming factor screened by the method, method of using the reprogramming factor, method of differentiating undifferentiated fused cells and method of constructing cell, tissues and organs
US7588937B2 (en) * 2001-10-03 2009-09-15 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
DE10162080A1 (en) * 2001-12-10 2003-06-26 Albrecht Mueller Process for the production of stem cells with increased development potential
WO2003064634A1 (en) * 2002-01-31 2003-08-07 Asahi Techno Glass Corporation Liquid for frozen storage of primate embryo stem cells and frozen storage method
WO2003081536A1 (en) 2002-03-26 2003-10-02 So-Woon Kim System and method for 3-dimension simulation of glasses
US7422736B2 (en) 2002-07-26 2008-09-09 Food Industry Research And Development Institute Somatic pluripotent cells
US20040048297A1 (en) * 2002-07-30 2004-03-11 Gene Logic, Inc. Nucleic acid detection assay control genes
JP3736517B2 (en) 2002-11-13 2006-01-18 学校法人近畿大学 Somatic cell nuclear reprogramming factor
AU2003901099A0 (en) 2003-03-11 2003-03-27 Es Cell International Pte Ltd. Methods of inducing differentiation of stem cells
US7462602B2 (en) * 2003-05-01 2008-12-09 University Of Florida Research Foundation, Inc. Anti-scarring ribozymes and methods
US9567591B2 (en) 2003-05-15 2017-02-14 Mello Biotechnology, Inc. Generation of human embryonic stem-like cells using intronic RNA
WO2004101775A1 (en) * 2003-05-16 2004-11-25 Kyowa Hakko Kogyo Co., Ltd. Novel stem cell derived from adult tissue and use thereof
JP4644663B2 (en) * 2003-06-03 2011-03-02 セル ジェネシス インコーポレイテッド Construction and method for enhanced expression of recombinant polypeptides from a single vector using peptide cleavage sites
WO2005010524A1 (en) * 2003-06-04 2005-02-03 Curis, Inc. Stem cell-based methods for identifying and characterizing agents
WO2005033297A1 (en) 2003-09-19 2005-04-14 The Rockefeller University Compositions, methods and kits relating to reprogramming adult differentiated cells and production of embryonic stem cell-like cells
JP2005095027A (en) 2003-09-22 2005-04-14 Reprocell Inc Undifferentiated state marker promoter of cell and its utilization
AU2004280066A1 (en) 2003-10-09 2005-04-21 Kyowa Hakko Kirin Co., Ltd. Genomically modified cell
WO2005047524A2 (en) * 2003-11-10 2005-05-26 The Scripps Research Institute Compositions and methods for inducing cell dedifferentiation
US7682828B2 (en) * 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
AU2004294835B2 (en) * 2003-12-01 2010-04-29 Technion Research & Development Foundation Ltd. Methods of generating stem cells and embryonic bodies carrying disease-causing mutations and methods of using same for studying genetic disorders
JP4340736B2 (en) * 2004-01-15 2009-10-07 国立大学法人福井大学 Vector incorporating a reporter gene
WO2005080598A1 (en) 2004-02-19 2005-09-01 Dainippon Sumitomo Pharma Co., Ltd. Method of screening somatic cell nucleus initializer
CA2560581C (en) 2004-03-23 2013-06-11 Toshihiro Akaike Pluripotent stem cell growing method
CA2568772A1 (en) * 2004-06-01 2005-12-15 San Diego State University Foundation Expression system based on recombinant vesicular stomatitis virus synthesizing t7 rna polymerase
EP1788079A4 (en) * 2004-07-08 2008-08-06 Japan Science & Tech Agency Animal tissue-eccentrically located pluripotent cell proliferating selectively in low-serium medium
WO2006084229A2 (en) 2004-07-15 2006-08-10 Primegen Biotech, Llc Use of nuclear material to therapeutically reprogram differentiated cells
WO2006017476A2 (en) * 2004-08-02 2006-02-16 The Research Foundation Of State University Of New York Amino functionalized ormosil nanoparticles as delivery vehicles
JPWO2006035741A1 (en) 2004-09-29 2008-05-15 伸弥 山中 ES cell specific expression gene and use thereof
AU2005326784B2 (en) 2004-10-08 2012-03-15 Virxsys Corporation Use of RNA trans-splicing for antibody gene transfer and antibody polypeptide production
US20060095319A1 (en) * 2004-10-29 2006-05-04 Cardwell Carlzo B Marketing and compensation method
US20060182724A1 (en) 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
US20080085555A1 (en) * 2005-02-28 2008-04-10 Takayuki Asahara Method For In Vitro Amplification Of Adult Stem Cells
US20070033061A1 (en) * 2005-04-05 2007-02-08 Achaogen, Inc. Business methods for commercializing antimicrobial and cytotoxic compounds
WO2007026255A2 (en) 2005-06-22 2007-03-08 Universitetet I Oslo Dedifferentiated cells and methods of making and using dedifferentiated cells
FR2888636B1 (en) * 2005-07-13 2007-09-28 Airbus France Sas DEVICE FOR AIDING AN APPROACH WITH VERTICAL GUIDE FOR AN AIRCRAFT
JP2009508470A (en) * 2005-07-21 2009-03-05 アボット・ラボラトリーズ Multiple gene expression including SORF constructs and polyprotein, proprotein and proteolytic methods
CA2659945C (en) 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
JP2009515515A (en) 2005-11-11 2009-04-16 ザ・ユニバーシティ・コート・オブ・ザ・ユニバーシティ・オブ・エディンバラ Cell reprogramming and genetic modification
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
BRPI0619794B8 (en) 2005-12-13 2022-06-14 Univ Kyoto Use of a reprogramming factor, agent for preparing an induced pluripotent stem cell from a somatic cell and methods for preparing an induced pluripotent stem cell method and for preparing a somatic cell and use of induced pluripotent stem cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8129187B2 (en) * 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
EP1987148A4 (en) 2006-02-27 2009-08-05 Imgen Co Ltd De-differentiation of astrocytes into neural stem cell using bmi-1
US20090252711A1 (en) 2006-05-11 2009-10-08 Andrew Craig Boquest Stem Cells And Methods Of Making And Using Stem Cells
US20090028835A1 (en) 2006-09-08 2009-01-29 Michigan State University Human transcriptome corresponding to human oocytes and use of said genes or the corresponding polypeptides to trans-differentiate somatic cells
JP2008099662A (en) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research Method for culturing stem cell
US20080132803A1 (en) * 2006-11-30 2008-06-05 Hyman Friedlander Method and system for doing business by mining the placental-chord complex
GB2458863B (en) * 2007-01-17 2011-10-12 Wisconsin Alumni Res Found Improved culture of stem cells
US8158415B2 (en) 2007-02-27 2012-04-17 Procell Therapeutics Inc. Combined use of cell permeable Nanog and Oct4 for increasing self-renewal and suppressing differentiation of stem cells
WO2008105566A1 (en) 2007-02-27 2008-09-04 Korea Stem Cell Bank System for providing stem cell services using internet and method thereof
SG193653A1 (en) * 2007-03-23 2013-10-30 Wisconsin Alumni Res Found Somatic cell reprogramming
WO2008124133A1 (en) 2007-04-07 2008-10-16 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
HUE030872T2 (en) 2007-05-29 2017-06-28 Christopher B Reid Methods for production and uses of multipotent cell populations, pluripotent cell populations, differentiated cell populations, and hiv-resistant cell populations
WO2008151058A2 (en) 2007-05-30 2008-12-11 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (en) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
US20120282229A1 (en) 2007-08-01 2012-11-08 Christian Kannemeier Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
WO2009023161A1 (en) 2007-08-10 2009-02-19 University Of Dayton Methods of producing pluripotent stem-like cells
ES2799897T3 (en) 2007-08-31 2020-12-22 Whitehead Inst Biomedical Res Stimulation of the wnt pathway in somatic cell reprogramming
AU2008297024B2 (en) 2007-10-31 2014-08-28 Kyoto University Nuclear reprogramming method
US20110151447A1 (en) 2007-11-06 2011-06-23 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells from non-embryonic human cells
WO2009067563A1 (en) 2007-11-19 2009-05-28 The Regents Of The University Of California Generation of pluripotent cells from fibroblasts
KR101532442B1 (en) * 2007-12-10 2015-06-29 고쿠리츠 다이가쿠 호진 교토 다이가쿠 Efficient method for nuclear reprogramming
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
US20090191171A1 (en) * 2008-01-18 2009-07-30 Yupo Ma Reprogramming of Differentiated Progenitor or Somatic Cells Using Homologous Recombination
KR101481164B1 (en) 2008-01-30 2015-01-09 주식회사 미래셀바이오 Method of manufacturing induced pluripotent stem cell originated from somatic cell
WO2009102983A2 (en) 2008-02-15 2009-08-20 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
EP2955222B1 (en) * 2008-03-17 2018-09-12 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
JP2011516042A (en) 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト(ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング) Vector and method for producing vector-free induced pluripotent stem (iPS) cells using site-specific recombination
CN101250502A (en) 2008-04-01 2008-08-27 中国科学院上海生命科学研究院 Method for preparing evoked pluripotent stem cell
CN101550406B (en) * 2008-04-03 2016-02-10 北京大学 Prepare the method for pluripotent stem cell, test kit and purposes
US20100021437A1 (en) * 2008-04-07 2010-01-28 The McLean Hospital Corporation Whitehead Institute for Biomedical Research Neural stem cells derived from induced pluripotent stem cells
ES2722198T3 (en) 2008-05-02 2019-08-08 Univ Kyoto Nuclear reprogramming method
EP2128245A1 (en) 2008-05-27 2009-12-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Generation of induced pluripotent stem (iPS) cells
KR101871192B1 (en) 2008-06-04 2018-06-27 후지필름 셀룰러 다이내믹스, 인코포레이티드 Methods for the production of iPS cells using non-viral approach
KR101372752B1 (en) 2008-07-31 2014-03-14 고꾸리츠 다이가꾸호오징 기후다이가꾸 Efficient method for establishing induced pluripotent stem cells
US8298825B1 (en) * 2008-08-25 2012-10-30 The General Hospital Corporation TGF-beta receptor inhibitors to enhance direct reprogramming
US20100062534A1 (en) * 2008-09-09 2010-03-11 The General Hospital Corporation Inducible lentiviral vectors for reprogramming somatic cells
SG10201600234PA (en) * 2008-10-24 2016-02-26 Wisconsin Alumni Res Found Pluripotent Stem Cells Obtained By Non-Viral Reprogramming

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