JP5827227B2 - グルコアミラーゼの変異体 - Google Patents

グルコアミラーゼの変異体 Download PDF

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Publication number
JP5827227B2
JP5827227B2 JP2012525164A JP2012525164A JP5827227B2 JP 5827227 B2 JP5827227 B2 JP 5827227B2 JP 2012525164 A JP2012525164 A JP 2012525164A JP 2012525164 A JP2012525164 A JP 2012525164A JP 5827227 B2 JP5827227 B2 JP 5827227B2
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JP
Japan
Prior art keywords
glucoamylase
seq
amino acid
starch
parent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012525164A
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English (en)
Japanese (ja)
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JP2013502211A (ja
JP2013502211A5 (enExample
Inventor
ペーター・エドヴァルド・デウン
リチャード・ボット
カスペル・ウィレム・フルーメン
マルテイン・シルファン・スフェッフェルス
ヴォルフガング・エーレ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International N&H Denmark ApS
Danisco US Inc
Original Assignee
DuPont Nutrition Biosciences ApS
Danisco US Inc
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Application filed by DuPont Nutrition Biosciences ApS, Danisco US Inc filed Critical DuPont Nutrition Biosciences ApS
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Publication of JP2013502211A5 publication Critical patent/JP2013502211A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012525164A 2009-08-19 2010-08-18 グルコアミラーゼの変異体 Expired - Fee Related JP5827227B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US23514009P 2009-08-19 2009-08-19
US61/235,140 2009-08-19
US36089110P 2010-07-01 2010-07-01
US61/360,891 2010-07-01
DKPA201070337 2010-07-15
DKPA201070337 2010-07-15
PCT/EP2010/062035 WO2011020852A1 (en) 2009-08-19 2010-08-18 Variants of glucoamylase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015145091A Division JP2016019522A (ja) 2009-08-19 2015-07-22 グルコアミラーゼの変異体

Publications (3)

Publication Number Publication Date
JP2013502211A JP2013502211A (ja) 2013-01-24
JP2013502211A5 JP2013502211A5 (enExample) 2014-08-07
JP5827227B2 true JP5827227B2 (ja) 2015-12-02

Family

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Family Applications (1)

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JP2012525164A Expired - Fee Related JP5827227B2 (ja) 2009-08-19 2010-08-18 グルコアミラーゼの変異体

Country Status (12)

Country Link
US (2) US8809023B2 (enExample)
EP (1) EP2467474A1 (enExample)
JP (1) JP5827227B2 (enExample)
CN (4) CN107299123A (enExample)
AR (1) AR077942A1 (enExample)
AU (1) AU2010284963C1 (enExample)
BR (1) BR112012003787A2 (enExample)
CA (1) CA2770607C (enExample)
EA (1) EA023194B1 (enExample)
MX (1) MX2012002032A (enExample)
PE (1) PE20121504A1 (enExample)
WO (2) WO2011020852A1 (enExample)

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CN107299123A (zh) * 2009-08-19 2017-10-27 杜邦营养生物科学有限公司 葡糖淀粉酶的变体
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US11421212B2 (en) 2016-08-05 2022-08-23 Cargill, Incorporated Engineered yeast strains with signal sequence-modified glucoamylase polypeptides and enhanced ethanol production
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RU2733294C1 (ru) 2016-11-18 2020-10-01 Балтика Брюэрис - Парт Оф Зе Карлсберг Груп Способ получения зернового солода и солодовый продукт, полученный таким способом
CN111971379B (zh) * 2018-02-28 2025-03-25 嘉吉公司 葡糖淀粉酶工程化酵母和发酵方法
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CN114457058B (zh) * 2021-10-19 2024-05-14 安徽农业大学 一种饲用α淀粉酶的突变改良方法及应用
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Publication number Publication date
CA2770607C (en) 2019-02-26
WO2012001139A1 (en) 2012-01-05
WO2012001139A9 (en) 2012-02-23
JP2013502211A (ja) 2013-01-24
EA023194B1 (ru) 2016-05-31
CN102575239B (zh) 2017-06-16
US9469848B2 (en) 2016-10-18
US20130309726A9 (en) 2013-11-21
US20120149070A1 (en) 2012-06-14
AU2010284963A1 (en) 2012-03-08
EP2467474A1 (en) 2012-06-27
MX2012002032A (es) 2012-03-26
US8809023B2 (en) 2014-08-19
US20130102035A1 (en) 2013-04-25
CN103119157A (zh) 2013-05-22
CN109295032A (zh) 2019-02-01
CN107299123A (zh) 2017-10-27
CN102575239A (zh) 2012-07-11
AU2010284963C1 (en) 2014-11-27
EA201270286A1 (ru) 2013-02-28
BR112012003787A2 (pt) 2017-07-04
WO2011020852A1 (en) 2011-02-24
CN103119157B (zh) 2018-10-09
CA2770607A1 (en) 2011-02-24
AR077942A1 (es) 2011-10-05
AU2010284963B2 (en) 2014-03-20
PE20121504A1 (es) 2012-11-05

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