AU2010284963C1 - Variants of glucoamylase - Google Patents

Variants of glucoamylase Download PDF

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Publication number
AU2010284963C1
AU2010284963C1 AU2010284963A AU2010284963A AU2010284963C1 AU 2010284963 C1 AU2010284963 C1 AU 2010284963C1 AU 2010284963 A AU2010284963 A AU 2010284963A AU 2010284963 A AU2010284963 A AU 2010284963A AU 2010284963 C1 AU2010284963 C1 AU 2010284963C1
Authority
AU
Australia
Prior art keywords
glucoamylase
seq
amino acid
variant
starch
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2010284963A
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English (en)
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AU2010284963A1 (en
AU2010284963B2 (en
Inventor
Wolfgang Aehle
Richard Bott
Peter Edvard Degn
Martijn Silvan Scheffers
Casper Willem Vroemen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International N&H Denmark ApS
Danisco US Inc
Original Assignee
DuPont Nutrition Biosciences ApS
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DuPont Nutrition Biosciences ApS, Danisco US Inc filed Critical DuPont Nutrition Biosciences ApS
Publication of AU2010284963A1 publication Critical patent/AU2010284963A1/en
Assigned to DUPONT NUTRITION BIOSCIENCES APS, DANISCO US INC. reassignment DUPONT NUTRITION BIOSCIENCES APS Amend patent request/document other than specification (104) Assignors: DANISCO A/S, DANISCO US INC.
Assigned to DANISCO US INC., DUPONT NUTRITION BIOSCIENCES APS reassignment DANISCO US INC. Amend patent request/document other than specification (104) Assignors: DANISCO US INC., DUPONT NUTRITION BIOSCIENCES APS
Application granted granted Critical
Publication of AU2010284963B2 publication Critical patent/AU2010284963B2/en
Publication of AU2010284963C1 publication Critical patent/AU2010284963C1/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00—Other raw materials for the preparation of beer
    • C12C5/004—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2010284963A 2009-08-19 2010-08-18 Variants of glucoamylase Ceased AU2010284963C1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US23514009P 2009-08-19 2009-08-19
US61/235,140 2009-08-19
US36089110P 2010-07-01 2010-07-01
US61/360,891 2010-07-01
DKPA201070337 2010-07-15
DKPA201070337 2010-07-15
PCT/EP2010/062035 WO2011020852A1 (en) 2009-08-19 2010-08-18 Variants of glucoamylase

Publications (3)

Publication Number Publication Date
AU2010284963A1 AU2010284963A1 (en) 2012-03-08
AU2010284963B2 AU2010284963B2 (en) 2014-03-20
AU2010284963C1 true AU2010284963C1 (en) 2014-11-27

Family

ID=42797171

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010284963A Ceased AU2010284963C1 (en) 2009-08-19 2010-08-18 Variants of glucoamylase

Country Status (12)

Country Link
US (2) US8809023B2 (enExample)
EP (1) EP2467474A1 (enExample)
JP (1) JP5827227B2 (enExample)
CN (4) CN107299123A (enExample)
AR (1) AR077942A1 (enExample)
AU (1) AU2010284963C1 (enExample)
BR (1) BR112012003787A2 (enExample)
CA (1) CA2770607C (enExample)
EA (1) EA023194B1 (enExample)
MX (1) MX2012002032A (enExample)
PE (1) PE20121504A1 (enExample)
WO (2) WO2011020852A1 (enExample)

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CN107299123A (zh) * 2009-08-19 2017-10-27 杜邦营养生物科学有限公司 葡糖淀粉酶的变体
RU2600885C2 (ru) * 2011-04-15 2016-10-27 Новозимс А/С Способ получения пивного сусла
DK2794641T3 (en) * 2011-12-22 2017-03-13 Dupont Nutrition Biosci Aps POLYPEPTIDES WITH GLUCOAMYLASE ACTIVITY AND PROCEDURES FOR PREPARING IT
US20150152458A1 (en) 2012-03-28 2015-06-04 Danisco Us Inc. Low temperature method for making high glucose syrup
EP2884853B1 (en) * 2012-08-14 2017-08-02 Danisco US Inc. Trichoderma reesei glucoamylase variants resistant to oxidation-related activity loss and the use thereof
MX2015002099A (es) * 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
BR112015008408A2 (pt) * 2012-10-17 2017-08-08 Novozymes As método de produção de um mosto cervejeiro
EP3027741B1 (en) * 2013-07-29 2019-10-23 Danisco US Inc. Gh61 enzyme variants
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US9988657B2 (en) 2014-02-27 2018-06-05 E I Du Pont De Nemours And Company Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
US20170321237A1 (en) 2014-12-01 2017-11-09 Novozymes A/S Improved Production of Glucose Syrups
US10612058B2 (en) * 2014-12-19 2020-04-07 Danisco Us Inc Methods for saccharifying a starch substrate
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WO2016160584A1 (en) 2015-03-27 2016-10-06 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
CN108431229A (zh) 2015-12-17 2018-08-21 嘉吉公司 糖转运蛋白修饰的酵母菌株和用于生物制品生产的方法
US10053678B2 (en) 2016-08-05 2018-08-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US11421212B2 (en) 2016-08-05 2022-08-23 Cargill, Incorporated Engineered yeast strains with signal sequence-modified glucoamylase polypeptides and enhanced ethanol production
US9670472B1 (en) 2016-08-05 2017-06-06 Fornia Biosolutions, Inc 2G16 glucoamylase compositions and methods
US10227578B2 (en) 2016-08-05 2019-03-12 Fornia Biosolutions, Inc. 2G16 glucoamylase compositions and methods
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
RU2733294C1 (ru) 2016-11-18 2020-10-01 Балтика Брюэрис - Парт Оф Зе Карлсберг Груп Способ получения зернового солода и солодовый продукт, полученный таким способом
CN111971379B (zh) * 2018-02-28 2025-03-25 嘉吉公司 葡糖淀粉酶工程化酵母和发酵方法
BR112020018294A2 (pt) * 2018-03-09 2020-12-29 Danisco Us Inc. Glucoamilases e métodos de uso dos mesmos
WO2020086430A2 (en) * 2018-10-22 2020-04-30 Dupont Nutrition Biosciences Aps Enzymes for infusion mashing in adjunct brewing technical field
US11535836B2 (en) 2018-12-21 2022-12-27 Fornia Biosolutions, Inc. Variant G6P G7P glucoamylase compositions and methods
CN115803421B (zh) * 2020-05-21 2025-07-15 国际营养与健康丹麦有限公司 用于使用高单宁材料进行酿造的不受抑制的淀粉酶
CN114457058B (zh) * 2021-10-19 2024-05-14 安徽农业大学 一种饲用α淀粉酶的突变改良方法及应用
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN120390792A (zh) 2022-12-19 2025-07-29 丹尼斯科美国公司 重组真菌菌株及其用于产生一致性蛋白质的方法
PL447340A1 (pl) * 2023-12-28 2025-06-30 Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie Zastosowanie preparatu enzymatycznego z Trichoderma reesei oraz kombinacji preparatów enzymatycznych z Trichoderma reesei i Aspergillus niger do zmiany aromatu piwa chmielonego na zimno

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Also Published As

Publication number Publication date
CA2770607C (en) 2019-02-26
WO2012001139A1 (en) 2012-01-05
WO2012001139A9 (en) 2012-02-23
JP2013502211A (ja) 2013-01-24
EA023194B1 (ru) 2016-05-31
CN102575239B (zh) 2017-06-16
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