JP4497923B2 - ロボット顕微鏡検査システム - Google Patents
ロボット顕微鏡検査システム Download PDFInfo
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| US8131053B2 (en) | 1999-01-25 | 2012-03-06 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
| US8406498B2 (en) | 1999-01-25 | 2013-03-26 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
| US8885913B2 (en) | 1999-01-25 | 2014-11-11 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
| US20060257884A1 (en) * | 2004-05-20 | 2006-11-16 | Amnis Corporation | Methods for preparing and analyzing cells having chromosomal abnormalities |
| US7450229B2 (en) | 1999-01-25 | 2008-11-11 | Amnis Corporation | Methods for analyzing inter-cellular phenomena |
| US8005314B2 (en) | 2005-12-09 | 2011-08-23 | Amnis Corporation | Extended depth of field imaging for high speed object analysis |
| JP3076568B1 (ja) * | 1999-07-15 | 2000-08-14 | 株式会社鈴木 | 電子部品用フイルムの孔明け加工方法 |
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| US8597597B2 (en) | 2003-06-26 | 2013-12-03 | Seng Enterprises Ltd. | Picoliter well holding device and method of making the same |
| US7888110B2 (en) * | 2003-06-26 | 2011-02-15 | Seng Enterprises Ltd. | Pico liter well holding device and method of making the same |
| US6873153B2 (en) * | 2003-07-07 | 2005-03-29 | Yeda Research And Development Co., Ltd. | Method and apparatus for acquiring multidimensional spectra and improved unidimensional spectra within a single scan |
| US20050051723A1 (en) * | 2003-07-23 | 2005-03-10 | Neagle Bradley D. | Examination systems for biological samples |
| US7372985B2 (en) * | 2003-08-15 | 2008-05-13 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
| JP4546741B2 (ja) * | 2004-01-08 | 2010-09-15 | オリンパス株式会社 | 蛍光顕微鏡 |
| JP3803673B2 (ja) * | 2004-02-02 | 2006-08-02 | オリンパス株式会社 | 測定方法及び測定装置 |
| US8953866B2 (en) | 2004-03-16 | 2015-02-10 | Amnis Corporation | Method for imaging and differential analysis of cells |
| US8150136B2 (en) * | 2004-03-16 | 2012-04-03 | Amnis Corporation | Image based quantitation of molecular translocation |
| CA2596101A1 (en) | 2004-03-16 | 2005-09-29 | Amnis Corporation | Method for imaging and differential analysis of cells |
| JP4405837B2 (ja) * | 2004-03-19 | 2010-01-27 | ヤマト科学株式会社 | 組織試料分析装置 |
| US7394943B2 (en) * | 2004-06-30 | 2008-07-01 | Applera Corporation | Methods, software, and apparatus for focusing an optical system using computer image analysis |
| JP2008505321A (ja) * | 2004-07-02 | 2008-02-21 | ブルーシフト・バイオテクノロジーズ・インコーポレーテッド | 蛍光体微小環境の探索 |
| US7403647B2 (en) * | 2004-09-13 | 2008-07-22 | Seng Enterprises Ltd. | Method for identifying an image of a well in an image of a well-bearing component |
| FI20040958L (fi) * | 2004-07-09 | 2006-01-10 | Chip Man Technologies Oy | Menetelmä ja laitteisto biologista analysointia varten |
| JP4752208B2 (ja) * | 2004-07-23 | 2011-08-17 | 株式会社ニコン | 光学顕微鏡システムおよびこれを用いた試料動画像生成方法 |
| US7490009B2 (en) * | 2004-08-03 | 2009-02-10 | Fei Company | Method and system for spectroscopic data analysis |
| JP4921978B2 (ja) * | 2004-11-09 | 2012-04-25 | 株式会社カネカ | 細胞培養装置、画像処理装置及び細胞検出システム |
| ES2352344T3 (es) | 2005-01-25 | 2011-02-17 | Seng Enterprises Limited | Dispositivo de microfluido para estudio de células. |
| JP4590601B2 (ja) * | 2005-03-08 | 2010-12-01 | 独立行政法人物質・材料研究機構 | ライブラリ分析装置 |
| US20070057211A1 (en) | 2005-05-25 | 2007-03-15 | Karsten Bahlman | Multifocal imaging systems and method |
| US7718131B2 (en) * | 2005-07-06 | 2010-05-18 | Genetix Limited | Methods and apparatus for imaging and processing of samples in biological sample containers |
| JP2008052227A (ja) * | 2005-09-15 | 2008-03-06 | Olympus Corp | 観察装置 |
| JP2007085927A (ja) * | 2005-09-22 | 2007-04-05 | Olympus Corp | 生体関連情報の画像化方法および生体内の相互作用を撮像する撮像方法、装置、装置を実行するためのプログラム、ソフトウェア、解析方法、試薬キット |
| US8288120B2 (en) | 2005-11-03 | 2012-10-16 | Seng Enterprises Ltd. | Method for studying floating, living cells |
| CN101438147B (zh) * | 2006-05-31 | 2011-09-28 | 奥林巴斯株式会社 | 生物试样摄像方法及生物试样摄像装置 |
| JP4431549B2 (ja) | 2006-05-31 | 2010-03-17 | 株式会社日立ハイテクノロジーズ | 蛍光分析装置 |
| JP5168819B2 (ja) * | 2006-06-02 | 2013-03-27 | 日産自動車株式会社 | 半導体パッケージおよびその製造方法 |
| EP2034349B2 (en) * | 2006-06-08 | 2023-07-19 | Nikon Corporation | Observing apparatus and observing method |
| WO2008019299A2 (en) * | 2006-08-04 | 2008-02-14 | Ikonisys, Inc. | Image processing method for a microscope system |
| JP5021254B2 (ja) * | 2006-09-06 | 2012-09-05 | オリンパス株式会社 | 顕微鏡装置の制御方法、顕微鏡装置 |
| CA2664135C (en) | 2006-09-22 | 2014-09-02 | Rafael Backhaus | Method and device for automated removal of cells and/or cell colonies |
| US9557217B2 (en) | 2007-02-13 | 2017-01-31 | Bti Holdings, Inc. | Universal multidetection system for microplates |
| CN101541943B (zh) * | 2007-02-28 | 2014-01-29 | 株式会社尼康 | 培养箱、预订时间管理方法和程序 |
| JP4853358B2 (ja) * | 2007-03-30 | 2012-01-11 | 株式会社日立プラントテクノロジー | 発光測定装置 |
| US20100195868A1 (en) * | 2007-05-31 | 2010-08-05 | Lu Peter J | Target-locking acquisition with real-time confocal (tarc) microscopy |
| WO2009016548A2 (en) | 2007-07-27 | 2009-02-05 | Koninklijke Philips Electronics N.V. | Method and system for imaging samples |
| DE102007043741A1 (de) * | 2007-09-10 | 2009-03-12 | Eppendorf Ag | Optisches Sensorsystem an einer Vorrichtung zur Behandlung von Flüssigkeiten |
| US8326014B2 (en) * | 2007-09-28 | 2012-12-04 | Cytyc Corporation | Methods and systems for processing biological specimens utilizing multiple wavelengths |
| US20090325211A1 (en) * | 2007-10-06 | 2009-12-31 | Ye Fang | System and method for dual-detection of a cellular response |
| WO2009081409A2 (en) | 2007-12-26 | 2009-07-02 | Seng Enterprises Ltd. | Device for the study of living cells |
| US9145540B1 (en) | 2007-11-15 | 2015-09-29 | Seng Enterprises Ltd. | Device for the study of living cells |
| AU2009212187B2 (en) * | 2008-02-06 | 2014-05-01 | Fei Company | A method and system for spectrum data analysis |
| JP5531379B2 (ja) * | 2008-02-26 | 2014-06-25 | 株式会社ニコン | インキュベータおよびインキュベータのスケジュール管理方法 |
| EP2289173B1 (en) * | 2008-05-15 | 2017-10-11 | Koninklijke Philips N.V. | Method, apparatus, and computer program product for compression and decompression of a gene sequencing image |
| JP4288323B1 (ja) | 2008-09-13 | 2009-07-01 | 独立行政法人科学技術振興機構 | 顕微鏡装置及びそれを用いた蛍光観察方法 |
| GB2464747B (en) * | 2008-10-10 | 2013-05-15 | Hai Kang Life Corp Ltd | Method for detection of analyte in microarray of samples and apparatus for performing such method |
| WO2010045949A2 (en) | 2008-10-21 | 2010-04-29 | Chemometec A/S | A method and apparatus for analysis of a particle |
| US8642352B2 (en) * | 2009-02-06 | 2014-02-04 | California Institute Of Technology | Methods and systems for detection of stoichiometry by Förster resonance energy transfer |
| FR2942899B1 (fr) * | 2009-03-03 | 2011-09-23 | Jose Balbuena | Dispositif et procede d'analyse optique de documents |
| DE102009012707A1 (de) | 2009-03-11 | 2010-09-16 | Carl Zeiss Microlmaging Gmbh | Mikroskop mit mehreren optischen Systemen im Abbildungsstrahlengang |
| US8300965B2 (en) * | 2009-03-24 | 2012-10-30 | General Electric Company | Methods and apparatus to perform multi-focal plane image acquisition and compression |
| JP5364430B2 (ja) * | 2009-04-22 | 2013-12-11 | オリンパス株式会社 | 細胞画像取得装置及び細胞画像取得方法 |
| JP4982523B2 (ja) * | 2009-05-11 | 2012-07-25 | 株式会社日立ハイテクノロジーズ | 蛍光分析方法，蛍光分析装置及び画像検出方法 |
| US20100295782A1 (en) | 2009-05-21 | 2010-11-25 | Yehuda Binder | System and method for control based on face ore hand gesture detection |
| JP5409120B2 (ja) * | 2009-05-27 | 2014-02-05 | キヤノン株式会社 | 補正方法、装置、及び、デバイス製造方法 |
| ES2972747T3 (es) | 2009-08-22 | 2024-06-14 | Ares Trading Sa | Obtención de imágenes y evaluación de embriones, ovocitos y células madre |
| US8451524B2 (en) | 2009-09-29 | 2013-05-28 | Amnis Corporation | Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile |
| US8817115B1 (en) | 2010-05-05 | 2014-08-26 | Amnis Corporation | Spatial alignment of image data from a multichannel detector using a reference image |
| US8971632B2 (en) * | 2010-08-19 | 2015-03-03 | Sharp Laboratories Of America, Inc. | System for feature detection for low contrast images |
| WO2012047678A2 (en) | 2010-09-27 | 2012-04-12 | Auxogyn, Inc. | Apparatus, method, and system for the automated imaging and evaluation of embryos, oocytes, and stem cells |
| US20150293026A1 (en) * | 2010-10-15 | 2015-10-15 | Dong-Guk Shin | Automated system for tissue histomorphometry |
| JP2012118126A (ja) * | 2010-11-29 | 2012-06-21 | Olympus Corp | 観察装置および観察方法 |
| JP5821206B2 (ja) * | 2011-02-09 | 2015-11-24 | 株式会社ニコン | 観察装置、画像観察方法、及び被検体 |
| CA2827945C (en) | 2011-02-23 | 2021-10-12 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of detecting aneuploidy in human embryos |
| WO2012142496A1 (en) | 2011-04-15 | 2012-10-18 | Constitution Medical, Inc. | Measuring volume and constituents of cells |
| FI20115483A0 (fi) * | 2011-05-19 | 2011-05-19 | Wallac Oy | Mittauslaite |
| EP2739587B1 (en) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Cell capture system |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| JP5818080B2 (ja) * | 2011-08-31 | 2015-11-18 | ソニー株式会社 | 画像処理装置および方法、記録媒体、並びに、プログラム |
| JP2013114042A (ja) * | 2011-11-29 | 2013-06-10 | Sony Corp | 画像取得装置、画像取得方法及び画像取得プログラム |
| CH706326A2 (de) * | 2012-03-14 | 2013-09-30 | Tecan Trading Ag | Verfahren und Mikroplatten-Reader zum Untersuchung von biologischen Zellen oder Zellkulturen. |
| CN103376249A (zh) * | 2012-04-28 | 2013-10-30 | 易尚明天科技有限公司 | 紫外点荧光扫描仪 |
| US8664595B2 (en) | 2012-06-28 | 2014-03-04 | Fei Company | Cluster analysis of unknowns in SEM-EDS dataset |
| US9188555B2 (en) | 2012-07-30 | 2015-11-17 | Fei Company | Automated EDS standards calibration |
| TW201415153A (zh) | 2012-10-01 | 2014-04-16 | Ind Tech Res Inst | 自動對焦系統與自動對焦方法 |
| US9778215B2 (en) | 2012-10-26 | 2017-10-03 | Fei Company | Automated mineral classification |
| US8937282B2 (en) | 2012-10-26 | 2015-01-20 | Fei Company | Mineral identification using mineral definitions including variability |
| US9048067B2 (en) | 2012-10-26 | 2015-06-02 | Fei Company | Mineral identification using sequential decomposition into elements from mineral definitions |
| US9091635B2 (en) | 2012-10-26 | 2015-07-28 | Fei Company | Mineral identification using mineral definitions having compositional ranges |
| EP2932425B1 (en) | 2012-12-14 | 2025-01-29 | The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone | Automated robotic microscopy systems |
| WO2014097991A1 (ja) * | 2012-12-18 | 2014-06-26 | コニカミノルタ株式会社 | 希少細胞検出装置、希少細胞検出方法、希少細胞観察システム、および細胞展開用デバイス |
| US9194829B2 (en) | 2012-12-28 | 2015-11-24 | Fei Company | Process for performing automated mineralogy |
| WO2014121200A1 (en) | 2013-02-01 | 2014-08-07 | Auxogyn, Inc. | Abnormal syngamy phenotypes observed with time lapse imaging for early identification of embryos with lower developmental potential |
| WO2014179269A2 (en) * | 2013-04-30 | 2014-11-06 | Molecular Devices, Llc | Apparatus and method for generating in-focus images using parallel imaging in a microscopy system |
| WO2015022781A1 (ja) * | 2013-08-15 | 2015-02-19 | コニカミノルタ株式会社 | 細胞検出方法および細胞検出装置 |
| GB201318919D0 (en) * | 2013-10-25 | 2013-12-11 | Isis Innovation | Compact microscope |
| US9714908B2 (en) | 2013-11-06 | 2017-07-25 | Fei Company | Sub-pixel analysis and display of fine grained mineral samples |
| JP6578950B2 (ja) * | 2014-01-07 | 2019-09-25 | ソニー株式会社 | 分析システム、分析プログラム及び分析方法 |
| CA2935944A1 (en) | 2014-01-09 | 2015-07-16 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Substituted benzoxazine and related compounds |
| DE102014003145A1 (de) | 2014-03-04 | 2015-09-10 | Carl Zeiss Microscopy Gmbh | Verfahren zur Korrektur der sphärischen Aberration bei mikroskopischen Anwendungen |
| EP3268715B1 (en) | 2015-03-11 | 2024-09-25 | TissueVision, Inc. | System and methods for serial staining and imaging |
| US10755078B2 (en) | 2015-04-10 | 2020-08-25 | President And Fellows Of Harvard College | Methods and devices for live cell imaging analysis |
| GB201507021D0 (en) | 2015-04-24 | 2015-06-10 | Isis Innovation | Compact microscope |
| US9939623B2 (en) * | 2015-10-19 | 2018-04-10 | Molecular Devices, Llc | Microscope system with transillumination-based autofocusing for photoluminescence imaging |
| EP3428263B1 (en) | 2016-03-11 | 2023-04-19 | Nikon Corporation | Evaluation device, observation device, and program |
| WO2018044683A1 (en) * | 2016-08-31 | 2018-03-08 | Molecular Devices, Llc | System and method for template-based image analysis |
| DE102016120726A1 (de) * | 2016-10-28 | 2018-05-03 | Als Automated Lab Solutions Gmbh | Vorrichtung und Verfahren zur Behandlung biologische Zellen enthaltender Proben, insbesondere von Blut- oder Zellproben |
| WO2018094290A1 (en) | 2016-11-18 | 2018-05-24 | Tissuevision, Inc. | Automated tissue section capture, indexing and storage system and methods |
| JP6928653B2 (ja) | 2017-06-26 | 2021-09-01 | オリンパス株式会社 | 細胞観察システム |
| CN110869485A (zh) * | 2017-06-26 | 2020-03-06 | 奥林巴斯株式会社 | 细胞观察系统 |
| WO2019046307A1 (en) | 2017-08-29 | 2019-03-07 | Celsee Diagnostics, Inc. | SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS |
| JPWO2019044408A1 (ja) * | 2017-08-31 | 2020-02-27 | 富士フイルム株式会社 | 画像処理装置、方法およびプログラム |
| GB201808312D0 (en) | 2018-05-21 | 2018-07-11 | Governing Council Of The Univ Of Toronto | A method for automated non-invasive measurement of sperm motility and morphology and automated selection of a sperm with high dna integrity |
| US12344827B2 (en) | 2018-07-17 | 2025-07-01 | Emory University | Automatized, programmable, high-throughput tissue culture and analysis systems and methods |
| WO2020154203A1 (en) | 2019-01-22 | 2020-07-30 | Applied Materials, Inc. | Capture and storage of magnified images |
| CN109784324B (zh) * | 2019-02-25 | 2023-08-04 | 广州牛顿光学研究院有限公司 | 一种机械自动控制盒统计细胞的方法 |
| US11255785B2 (en) | 2019-03-14 | 2022-02-22 | Applied Materials, Inc. | Identifying fiducial markers in fluorescence microscope images |
| WO2020186029A1 (en) | 2019-03-14 | 2020-09-17 | Applied Materials, Inc. | Identifying fiducial markers in microscope images |
| JPWO2020202701A1 (ja) * | 2019-03-29 | 2021-12-23 | 富士フイルム株式会社 | 細胞評価装置、細胞評価装置の作動方法、細胞評価装置の作動プログラム、並びに細胞培養システム |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| US11578322B2 (en) | 2019-05-07 | 2023-02-14 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| US11327084B2 (en) * | 2019-09-19 | 2022-05-10 | Invidx Corp. | Joint hematology and biochemistry point-of-care testing system |
| US11161109B2 (en) | 2019-09-19 | 2021-11-02 | Invidx Corp. | Point-of-care testing cartridge with sliding cap |
| EP4035127A4 (en) | 2019-09-24 | 2023-10-18 | Applied Materials, Inc. | INTERACTIVE TRAINING OF A MACHINE LEARNING MODEL FOR TISSUE SEGMENTATION |
| WO2021127019A1 (en) | 2019-12-17 | 2021-06-24 | Applied Materials, Inc. | System and method for acquisition and processing of multiplexed fluorescence in-situ hybridization images |
| EP4078441A1 (en) * | 2019-12-20 | 2022-10-26 | Genentech, Inc. | Cell line development image characterization with convolutional neural networks |
| GB2596145A (en) * | 2020-06-19 | 2021-12-22 | Oxford Nanoimaging Ltd | A optical imaging method |
| US20240069315A1 (en) * | 2021-01-06 | 2024-02-29 | Scopgenx Private Limited | A compact portable multimodal microscopy |
| US20240411121A1 (en) * | 2021-01-25 | 2024-12-12 | Hewlett-Packard Development Company, L.P. | Single point sensing |
| CN113109352A (zh) * | 2021-04-07 | 2021-07-13 | 桂林电子科技大学 | 一种基于机器视觉的生丝质量检测方法及装置 |
| DE102022112378B4 (de) * | 2022-05-17 | 2024-02-15 | Abberior Instruments Gmbh | Verfahren, lichtmikroskop und computerprogramm zur bestimmung eines referenzzeitpunktes |
| WO2024138094A1 (en) * | 2022-12-22 | 2024-06-27 | Eikon Therapeutics, Inc. | Oblique line scanner systems and methods for high throughput single molecule tracking in living cells |
| CN117969467A (zh) * | 2023-11-23 | 2024-05-03 | 中国人民解放军空军军医大学 | 辅助实现材料表面活细胞多视野同时实时动态观察的装置 |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4000417A (en) * | 1975-08-25 | 1976-12-28 | Honeywell Inc. | Scanning microscope system with automatic cell find and autofocus |
| US4012112A (en) | 1975-08-25 | 1977-03-15 | Honeywell Inc. | Microscope stage positioning system |
| JPS53135697A (en) | 1977-04-30 | 1978-11-27 | Olympus Optical Co Ltd | Automatic cell diagnosis apparatus |
| US4513438A (en) | 1982-04-15 | 1985-04-23 | Coulter Electronics, Inc. | Automated microscopy system and method for locating and re-locating objects in an image |
| JPS60205411A (ja) | 1984-03-29 | 1985-10-17 | Olympus Optical Co Ltd | 倒立型顕微鏡 |
| US5109429A (en) | 1985-11-04 | 1992-04-28 | Cell Analysis Systems,Inc. | Apparatus and method for analyses of biological specimens |
| US4705949A (en) | 1985-11-25 | 1987-11-10 | The United States Of America As Represented By The Secretary Of Commerce | Method and apparatus relating to specimen cells for scanning electron microscopes |
| US4833382A (en) | 1986-06-06 | 1989-05-23 | Gibbs David L | Method and apparatus for use in microscope investigations |
| JPS63167313A (ja) | 1986-12-27 | 1988-07-11 | Hitachi Ltd | 自動焦点制御方法 |
| US4974952A (en) | 1988-03-31 | 1990-12-04 | Focht Daniel C | Live cell chamber for microscopes |
| DE3828381C2 (de) | 1988-08-20 | 1997-09-11 | Zeiss Carl Fa | Verfahren und Einrichtung zur automatischen Fokussierung eines optischen Systems |
| US5073857A (en) | 1989-06-01 | 1991-12-17 | Accuron Corporation | Method and apparatus for cell analysis |
| US5480804A (en) | 1989-06-28 | 1996-01-02 | Kirin Beverage Corporation | Method of and apparatus for detecting microorganisms |
| US5991028A (en) | 1991-02-22 | 1999-11-23 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for cell classification |
| CA2077781A1 (en) | 1991-09-23 | 1993-03-24 | James W. Bacus | Method and apparatus for automated assay of biological specimens |
| JP2726346B2 (ja) | 1991-12-25 | 1998-03-11 | 佐原 今朝徳 | 手術用顕微鏡の自動焦点機構 |
| US5459577A (en) * | 1992-06-01 | 1995-10-17 | Nikon Corporation | Method of and apparatus for measuring pattern positions |
| US5594235A (en) | 1993-06-17 | 1997-01-14 | Ultrapointe Corporation | Automated surface acquisition for a confocal microscope |
| US5574594A (en) | 1993-11-05 | 1996-11-12 | Nikon Inc. | Automated microscope slide marking device |
| JPH07318807A (ja) | 1994-05-19 | 1995-12-08 | Nikon Corp | 自動顕微鏡 |
| US6483948B1 (en) | 1994-12-23 | 2002-11-19 | Leica Ag | Microscope, in particular a stereomicroscope, and a method of superimposing two images |
| US6319668B1 (en) * | 1995-04-25 | 2001-11-20 | Discovery Partners International | Method for tagging and screening molecules |
| IT1274405B (it) | 1995-04-28 | 1997-07-17 | San Raffaele Centro Fond | Dispositivo di posizionamento e centramento automatico di testa ottica di microscopio |
| CA2227177A1 (en) | 1995-07-19 | 1997-02-06 | Morphometrix Technologies Inc. | Automated scanning of microscope slides |
| US6005964A (en) | 1996-01-24 | 1999-12-21 | The Board Of Trustees Of The University Of Illinois | Automatic machine vision microscope slide inspection system and method |
| DE19616997A1 (de) | 1996-04-27 | 1997-10-30 | Boehringer Mannheim Gmbh | Verfahren zur automatisierten mikroskopunterstützten Untersuchung von Gewebeproben oder Körperflüssigkeitsproben |
| DE19709348C2 (de) | 1996-05-29 | 1999-07-01 | Schubert Walter Dr Md | Automatisches Multi-Epitop-Ligand-Kartierungsverfahren |
| US6031930A (en) | 1996-08-23 | 2000-02-29 | Bacus Research Laboratories, Inc. | Method and apparatus for testing a progression of neoplasia including cancer chemoprevention testing |
| KR100556864B1 (ko) | 1997-05-13 | 2006-03-10 | 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 | 렌티바이러스-기원 유전자 전달 벡터 |
| US6517781B1 (en) * | 1997-06-02 | 2003-02-11 | Aurora Biosciences Corporation | Low fluorescence assay platforms and related methods for drug discovery |
| WO2000000600A2 (en) | 1997-09-22 | 2000-01-06 | Chang Lung Ji | Lentiviral vectors, comprising modified major donor splice sites and major packaging signals |
| WO1999021042A2 (en) | 1997-10-17 | 1999-04-29 | Accumed International, Inc. | High-precision computer-aided microscope system |
| US6285498B1 (en) | 1997-10-17 | 2001-09-04 | Accumed International, Inc. | High-precision computer-aided microscope system |
| JP4322971B2 (ja) | 1998-01-06 | 2009-09-02 | オリンパス株式会社 | 顕微鏡用のオートフォーカス装置 |
| JPH11344675A (ja) * | 1998-05-29 | 1999-12-14 | Nikon Corp | 倒立顕微鏡 |
| JP2000162506A (ja) | 1998-11-30 | 2000-06-16 | Tokyo Seimitsu Co Ltd | 共焦点顕微鏡 |
| US6130745A (en) * | 1999-01-07 | 2000-10-10 | Biometric Imaging, Inc. | Optical autofocus for use with microtiter plates |
| US6986993B1 (en) * | 1999-08-05 | 2006-01-17 | Cellomics, Inc. | System for cell-based screening |
| AU6522300A (en) * | 1999-08-05 | 2001-03-05 | Cellomics, Inc. | Optical system analysis of cells |
| JP3736278B2 (ja) * | 2000-04-12 | 2006-01-18 | 松下電器産業株式会社 | 生化学物質の観察方法 |
| JP2001305058A (ja) * | 2000-04-26 | 2001-10-31 | Hitachi Ltd | 化学分析装置 |
| US6818403B2 (en) * | 2000-11-08 | 2004-11-16 | Surface Logix, Inc. | Method of monitoring haptotaxis |
-
2002
- 2002-12-05 EP EP02792332.5A patent/EP1461592B1/en not_active Expired - Lifetime
- 2002-12-05 US US10/313,942 patent/US7139415B2/en not_active Expired - Lifetime
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- 2002-12-05 CA CA2468861A patent/CA2468861C/en not_active Expired - Lifetime
- 2002-12-05 AU AU2002357791A patent/AU2002357791B9/en not_active Expired
- 2002-12-05 WO PCT/US2002/039033 patent/WO2003048705A1/en not_active Ceased
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