JP2020501573A5 - - Google Patents
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- JP2020501573A5 JP2020501573A5 JP2019533058A JP2019533058A JP2020501573A5 JP 2020501573 A5 JP2020501573 A5 JP 2020501573A5 JP 2019533058 A JP2019533058 A JP 2019533058A JP 2019533058 A JP2019533058 A JP 2019533058A JP 2020501573 A5 JP2020501573 A5 JP 2020501573A5
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- enzyme
- recombinant
- polymerization
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 108090000790 Enzymes Proteins 0.000 claims 41
- 102000004190 Enzymes Human genes 0.000 claims 41
- 150000001413 amino acids Chemical class 0.000 claims 25
- 238000006116 polymerization reaction Methods 0.000 claims 23
- 230000000379 polymerizing effect Effects 0.000 claims 14
- 238000006467 substitution reaction Methods 0.000 claims 11
- 125000003275 alpha amino acid group Chemical group 0.000 claims 9
- 230000037430 deletion Effects 0.000 claims 7
- 238000012217 deletion Methods 0.000 claims 7
- 230000037431 insertion Effects 0.000 claims 6
- 238000003780 insertion Methods 0.000 claims 6
- 238000012986 modification Methods 0.000 claims 4
- 230000004048 modification Effects 0.000 claims 4
- 102100022840 DnaJ homolog subfamily C member 7 Human genes 0.000 claims 3
- 108060002716 Exonuclease Proteins 0.000 claims 3
- 101000903053 Homo sapiens DnaJ homolog subfamily C member 7 Proteins 0.000 claims 3
- 101000847024 Homo sapiens Tetratricopeptide repeat protein 1 Proteins 0.000 claims 3
- 102100032841 Tetratricopeptide repeat protein 1 Human genes 0.000 claims 3
- 239000001768 carboxy methyl cellulose Substances 0.000 claims 3
- 230000008878 coupling Effects 0.000 claims 3
- 238000010168 coupling process Methods 0.000 claims 3
- 238000005859 coupling reaction Methods 0.000 claims 3
- 102000013165 exonuclease Human genes 0.000 claims 3
- 210000003811 finger Anatomy 0.000 claims 3
- 239000004312 hexamethylene tetramine Substances 0.000 claims 3
- 239000001103 potassium chloride Substances 0.000 claims 3
- 239000001120 potassium sulphate Substances 0.000 claims 3
- 230000035772 mutation Effects 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 210000003813 thumb Anatomy 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023000897A JP2023026651A (ja) | 2016-12-19 | 2023-01-06 | シークエンシング反応用の重合酵素 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662436410P | 2016-12-19 | 2016-12-19 | |
| US62/436,410 | 2016-12-19 | ||
| PCT/US2017/067427 WO2018118997A2 (en) | 2016-12-19 | 2017-12-19 | Polymerizing enzymes for sequencing reactions |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023000897A Division JP2023026651A (ja) | 2016-12-19 | 2023-01-06 | シークエンシング反応用の重合酵素 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020501573A JP2020501573A (ja) | 2020-01-23 |
| JP2020501573A5 true JP2020501573A5 (enExample) | 2021-02-04 |
Family
ID=61148468
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019533058A Pending JP2020501573A (ja) | 2016-12-19 | 2017-12-19 | シークエンシング反応用の重合酵素 |
| JP2023000897A Pending JP2023026651A (ja) | 2016-12-19 | 2023-01-06 | シークエンシング反応用の重合酵素 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023000897A Pending JP2023026651A (ja) | 2016-12-19 | 2023-01-06 | シークエンシング反応用の重合酵素 |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11312944B2 (enExample) |
| EP (1) | EP3555272A2 (enExample) |
| JP (2) | JP2020501573A (enExample) |
| KR (2) | KR20190093659A (enExample) |
| CN (1) | CN110268055A (enExample) |
| AU (1) | AU2017378990B2 (enExample) |
| BR (1) | BR112019012181A2 (enExample) |
| CA (1) | CA3047321A1 (enExample) |
| MX (1) | MX2019007248A (enExample) |
| TW (1) | TWI788318B (enExample) |
| WO (1) | WO2018118997A2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3555314B1 (en) | 2016-12-19 | 2023-08-16 | Quantum-Si Incorporated | Loading molecules into sample wells for analysis |
| WO2018118997A2 (en) * | 2016-12-19 | 2018-06-28 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| CA3059211A1 (en) | 2017-05-05 | 2018-11-08 | Quantum-Si Incorporated | Substrates having modified surface reactivity and antifouling properties in biological reactions |
| IL276343B2 (en) | 2018-01-29 | 2024-10-01 | St Jude Childrens Res Hospital Inc | Method for nucleic acid amplification |
| US10934533B1 (en) | 2018-10-10 | 2021-03-02 | New England Biolabs, Inc. | Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA |
| CN113597468B (zh) * | 2019-03-13 | 2024-09-13 | 武汉爱博泰克生物科技有限公司 | 用于快速扩增的突变型Taq聚合酶 |
| JP2022533366A (ja) * | 2019-05-17 | 2022-07-22 | ４ベースバイオ ソシエダー リミターダ | プライマー認識が改良されたＰｈｉ２９ ＤＮＡポリメラーゼ変異体 |
| EP3976771A1 (en) * | 2019-06-28 | 2022-04-06 | Quantum-si Incorporated | Polymerizing enzymes for sequencing reactions |
| BR112022006682A2 (pt) | 2019-10-11 | 2022-07-12 | Quantum Si Inc | Modificação de superfície na fase de vapor |
| EP4103745A4 (en) * | 2020-02-10 | 2024-03-13 | Bioskryb Genomics, Inc. | Phi29 mutants and use thereof |
| AU2021401791A1 (en) | 2020-12-15 | 2023-07-06 | Quantum-Si Incorporated | Molecular barcode analysis by single-molecule kinetics |
| CN116083393B (zh) * | 2023-02-14 | 2025-09-23 | 南方科技大学 | 一种dna装配分子机器及其应用 |
| WO2025067248A1 (zh) * | 2023-09-25 | 2025-04-03 | 成都今是科技有限公司 | 用于单分子测序的核酸聚合酶及其突变株 |
| CN119082075B (zh) * | 2024-09-12 | 2025-06-27 | 湖南艾科瑞生物工程有限公司 | 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用 |
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| DE4435728A1 (de) | 1994-01-19 | 1995-07-20 | Boehringer Mannheim Gmbh | Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| GB2333597B (en) | 1996-10-29 | 2001-04-18 | Univ Nebraska At Lincoln | Method for detecting point mutations in dna utilizing fluorescence energy transfer |
| DE19810879A1 (de) | 1998-03-13 | 1999-09-16 | Roche Diagnostics Gmbh | Polymerasenchimären |
| US6153442A (en) | 1998-05-20 | 2000-11-28 | Dade Behring Inc. | Reagents and methods for specific binding assays |
| US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| JP4638043B2 (ja) | 1998-12-14 | 2011-02-23 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法 |
| US6914128B1 (en) | 1999-03-25 | 2005-07-05 | Abbott Gmbh & Co. Kg | Human antibodies that bind human IL-12 and methods for producing |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| US6869764B2 (en) | 2000-06-07 | 2005-03-22 | L--Cor, Inc. | Nucleic acid sequencing using charge-switch nucleotides |
| US6936702B2 (en) | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
| CA2314398A1 (en) | 2000-08-10 | 2002-02-10 | Edward Shipwash | Microarrays and microsystems for amino acid analysis and protein sequencing |
| IL158418A0 (en) | 2001-04-19 | 2004-05-12 | Scripps Research Inst | In vivo incorporation of unnatural amino acids |
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| WO2005044836A2 (de) | 2003-11-05 | 2005-05-19 | Genovoxx Gmbh | Makromolekulare nukleotidverbindungen und methoden zu deren anwendung |
| US7462452B2 (en) | 2004-04-30 | 2008-12-09 | Pacific Biosciences Of California, Inc. | Field-switch sequencing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| ATE481505T1 (de) | 2005-12-12 | 2010-10-15 | Us Gov Health & Human Serv | Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren |
| CA2633524A1 (en) * | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Polymerases for nucleotide analogue incorporation |
| US8084734B2 (en) | 2006-05-26 | 2011-12-27 | The George Washington University | Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays |
| US8617811B2 (en) | 2008-01-28 | 2013-12-31 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| WO2009114182A1 (en) | 2008-03-13 | 2009-09-17 | Pacific Biosciences Of California, Inc. | Labeled reactants and their uses |
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| US8999676B2 (en) | 2008-03-31 | 2015-04-07 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for improved single molecule sequencing |
| US8257954B2 (en) | 2008-03-31 | 2012-09-04 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| US9127259B2 (en) * | 2008-03-31 | 2015-09-08 | Pacific Biosciences Of California, Inc. | Enzymes resistant to photodamage |
| US8420366B2 (en) | 2008-03-31 | 2013-04-16 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
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| KR101063981B1 (ko) | 2009-01-12 | 2011-09-14 | 서강대학교산학협력단 | 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법 |
| DE102009013653B4 (de) | 2009-03-18 | 2014-09-18 | Bruker Daltonik Gmbh | Protein-Sequenzierung mit MALDI-Massenspektrometrie |
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| WO2011040971A2 (en) * | 2009-09-30 | 2011-04-07 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
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| EP2689028B1 (en) | 2011-03-23 | 2017-08-30 | Pacific Biosciences Of California, Inc. | Isolation of polymerase-nucleic acid complexes and loading onto substrates |
| WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
| US8906660B2 (en) * | 2012-02-01 | 2014-12-09 | Pacific Biosciences Of California, Inc. | Recombinant polymerases with increased phototolerance |
| EP3590948A1 (en) | 2012-02-15 | 2020-01-08 | Pacific Biosciences of California, Inc. | Polymerase enzyme substrates with protein shield |
| US9399766B2 (en) * | 2012-10-01 | 2016-07-26 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for incorporation of protein shield nucleotide analogs |
| US9435810B2 (en) | 2013-03-15 | 2016-09-06 | Washington University | Molecules and methods for iterative polypeptide analysis and processing |
| US9678080B2 (en) | 2013-06-14 | 2017-06-13 | Pacific Biosciences Of California, Inc. | Bis-biotinylation tags |
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| US11268963B2 (en) | 2015-10-16 | 2022-03-08 | The Governing Council Of The University Of Toronto | Protein sequencing methods and reagents |
| CN108350491A (zh) | 2015-11-18 | 2018-07-31 | 加利福尼亚太平洋生物科学股份有限公司 | 将核酸加载到基材上 |
| US10676788B2 (en) | 2015-11-20 | 2020-06-09 | Pacific Biosciences Of California, Inc. | Modified nucleotide reagents |
| WO2018118997A2 (en) * | 2016-12-19 | 2018-06-28 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| EP3655408B1 (en) | 2017-08-24 | 2021-07-14 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | Conformational restriction of cyanine fluorophores in far-red and near-ir range |
| US10836798B2 (en) | 2018-11-08 | 2020-11-17 | Government Of The United States Of America, As Represented By The Secretary Of Commerce | Amino acid-specific binder and selectively identifying an amino acid |
| EP3976771A1 (en) * | 2019-06-28 | 2022-04-06 | Quantum-si Incorporated | Polymerizing enzymes for sequencing reactions |
-
2017
- 2017-12-19 WO PCT/US2017/067427 patent/WO2018118997A2/en not_active Ceased
- 2017-12-19 CA CA3047321A patent/CA3047321A1/en active Pending
- 2017-12-19 MX MX2019007248A patent/MX2019007248A/es unknown
- 2017-12-19 KR KR1020197020661A patent/KR20190093659A/ko not_active Ceased
- 2017-12-19 CN CN201780077928.1A patent/CN110268055A/zh active Pending
- 2017-12-19 BR BR112019012181-1A patent/BR112019012181A2/pt not_active Application Discontinuation
- 2017-12-19 KR KR1020237012002A patent/KR20230052995A/ko not_active Ceased
- 2017-12-19 AU AU2017378990A patent/AU2017378990B2/en active Active
- 2017-12-19 JP JP2019533058A patent/JP2020501573A/ja active Pending
- 2017-12-19 EP EP17838018.4A patent/EP3555272A2/en not_active Withdrawn
- 2017-12-19 TW TW106144668A patent/TWI788318B/zh active
- 2017-12-19 US US15/846,967 patent/US11312944B2/en active Active
-
2022
- 2022-03-09 US US17/690,920 patent/US12545901B2/en active Active
-
2023
- 2023-01-06 JP JP2023000897A patent/JP2023026651A/ja active Pending
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