CN110268055A - 用于测序反应的聚合酶 - Google Patents
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
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- C12P19/30—Nucleotides
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12Y—ENZYMES
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2024168961A1 (zh) * | 2023-02-14 | 2024-08-22 | 南方科技大学 | 一种dna装配分子机器及其应用 |
| CN119082075A (zh) * | 2024-09-12 | 2024-12-06 | 湖南艾科瑞生物工程有限公司 | 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用 |
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| AU2017378985A1 (en) | 2016-12-19 | 2019-06-20 | Quantum-Si Incorporated | Loading molecules into sample wells for analysis |
| AU2017378990B2 (en) * | 2016-12-19 | 2024-05-16 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| BR112019021889A2 (pt) | 2017-05-05 | 2020-05-26 | Quantum-Si Incorporated | Substratos tendo reatividade de superfície modificada e propriedades anti-incrustantes em reações biológicas |
| US10934533B1 (en) | 2018-10-10 | 2021-03-02 | New England Biolabs, Inc. | Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA |
| WO2020185702A2 (en) * | 2019-03-13 | 2020-09-17 | Abclonal Science, Inc. | Mutant taq polymerase for faster amplification |
| CN114174502B (zh) * | 2019-05-17 | 2025-09-19 | 4贝思生物有限公司 | 具有改进的引物识别的Phi29 DNA聚合酶突变体 |
| AU2020302099A1 (en) * | 2019-06-28 | 2022-01-27 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| CA3157505A1 (en) | 2019-10-11 | 2021-04-15 | Quantum-Si Incorporated | Surface modification in the vapor phase |
| CA3170318A1 (en) * | 2020-02-10 | 2021-08-19 | BioSkryb Genomics, Inc. | Phi29 mutants and use thereof |
| CA3177753A1 (en) | 2020-12-15 | 2022-06-23 | Quantum-Si Incorporated | Molecular barcode analysis by single-molecule kinetics |
| WO2025067248A1 (zh) * | 2023-09-25 | 2025-04-03 | 成都今是科技有限公司 | 用于单分子测序的核酸聚合酶及其突变株 |
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| CN101365807A (zh) * | 2005-12-22 | 2009-02-11 | 加利福尼亚太平洋生物科学股份有限公司 | 用于掺入核苷酸类似物的聚合酶 |
| CN102257136A (zh) * | 2008-11-03 | 2011-11-23 | 卡帕生物系统 | 嵌合dna聚合酶 |
| CN102648275A (zh) * | 2009-07-02 | 2012-08-22 | 康斯乔最高科学研究公司 | 噬菌体φ29DNA聚合酶嵌合体 |
| WO2011040971A2 (en) * | 2009-09-30 | 2011-04-07 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| US20130217007A1 (en) * | 2012-02-01 | 2013-08-22 | Pacific Biosciences Of California, Inc. | Recombinant Polymerases With Increased Phototolerance |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024168961A1 (zh) * | 2023-02-14 | 2024-08-22 | 南方科技大学 | 一种dna装配分子机器及其应用 |
| CN119082075A (zh) * | 2024-09-12 | 2024-12-06 | 湖南艾科瑞生物工程有限公司 | 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用 |
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| AU2017378990A1 (en) | 2019-06-20 |
| EP3555272A2 (en) | 2019-10-23 |
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